LOCUS       DASTIB010000001         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_119182, whole genome shotgun sequence.
ACCESSION   DASTIB010000001 DASTIB010000000
VERSION     DASTIB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119182"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..516)
                     /locus_tag="VFP93_00005"
     CDS             complement(121..516)
                     /locus_tag="VFP93_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843020.1"
                     /translation="MKNYSLTLMLIVLNVPAYAEIPQLAPIKISRVKAPYFLEAISED
                     MKAFSIDNQKGKSKNQEQKVIDFETNDIDIGFIARNAALLRENMCSIIGDGEIELSLS
                     GKSETGLIIVNSEIEAGIKVTFICNNEQF"
     gene            complement(524..1054)
                     /locus_tag="VFP93_00010"
     CDS             complement(524..1054)
                     /locus_tag="VFP93_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843021.1"
                     /translation="MIVQVKLQGIAQFQKIMANIPTKVERKAQQALNRTLTRSRTQMT
                     NSIGEKLNLKKKTIRELIFLKNANKNTGLIGSLIVKPRLVSLIEYNAKQNRKGVSFKI
                     FKNRPRQTINQAFIFPDSRGRLTVFKRETKARLKIKPLYGTRAVDAAKDSLTKVKVFA
                     EQFFQAEIKRMVNLKI"
     gene            complement(1054..1449)
                     /locus_tag="VFP93_00015"
     CDS             complement(1054..1449)
                     /locus_tag="VFP93_00015"
                     /inference="COORDINATES: protein motif:HMM:NF022795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HET9843022.1"
                     /translation="MKLLKIFSSAIKANSIEAVGTIIDKVFPIEEEKQKAQTVIDKLK
                     QHPKELQFLLNLLHDKGRSIYLAGWRPFIGWTCGLGLANVFVINPWLQWLVGIDGPRM
                     PLDVIVELIVTMLGLGLMRTAEKLGGKTK"
     gene            complement(1449..1832)
                     /locus_tag="VFP93_00020"
     CDS             complement(1449..1832)
                     /locus_tag="VFP93_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HET9843023.1"
                     /translation="MSWRFFSEKEMACRCGCAKVEMNNNFMNKLIALREHLHFPLPIT
                     SGFRCAKHNSTVSKTSQSGPHTLGRAVDISICGENAFKLIQNAPSFGFTGFGISQKGP
                     QSVRFVHIDDLTKEDGFPRPWIWGY"
     gene            complement(1829..2125)
                     /locus_tag="VFP93_00025"
     CDS             complement(1829..2125)
                     /locus_tag="VFP93_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843024.1"
                     /translation="MAKISKGWVFDKNINLSNLLTALVIAVSVIWWAAQIEKRIAVLE
                     IELKAFYEAQIQIKAMQLHRDKKQDDEVQRLDTVMQASLNRLESKVDKLLTSKL"
BASE COUNT          762 a          509 c          400 g          875 t
ORIGIN      
        1 tcctttgagt cgctaagaaa accttattta aatcaaccat atattaattc caggatattt
       61 cttttcgttc ataataatat agagcaaaaa aacaatcatt gttaattcaa tttttttcga
      121 ttaaaattgt tcattattac aaataaaagt aactttaatc ccagcctcaa tctcagaatt
      181 tacaattatc aaccctgttt cagatttgcc gcttagcgat aactctattt ctccatcgcc
      241 aataatacta cacatattct ctctaagtaa tgcagcattt cttgcaataa acccgatgtc
      301 gatatcgttc gtttcaaaat cgattacttt ttgctcttga ttttttgact tccctttttg
      361 attatctatc gaaaaggctt tcatatcttc tgaaattgct tcaagaaaat atggcgcttt
      421 aactcttgaa attttaattg gcgccaattg aggtatttca gcataagcag gtacattcaa
      481 aacaattaac atcaatgtca aagaataatt tttcatgcat catctaaatt tttaagttga
      541 ccattcgttt aatttcagct tgaaaaaatt gctccgcaaa aaccttcacc ttcgttaaac
      601 tatctttcgc agcatcaaca gcccgcgtgc catatagtgg cttaatcttc agtctcgcct
      661 tcgtttctct tttaaaaacc gtaagtctgc ccctactatc aggaaatata aaagcttgat
      721 ttatggtttg ccttggtcga tttttaaata tcttaaagct cacacctttg cggttctgct
      781 tggcgttata ttcaatcaag gataccaacc gcggcttaac tattaaactc ccaataaggc
      841 ctgtattttt attcgcattt tttagaaaga tgagttcccg tattgttttc tttttaagat
      901 ttagcttttc accaatggaa ttggtcatct gggtgcgaga tcgcgttagg gtgcgattga
      961 gagcttgttg tgcttttctt tccacttttg tgggaatatt tgccattatc ttttgaaatt
     1021 gcgcaattcc ttgtaatttt acttgtacaa tcattatttt gttttaccac caagtttctc
     1081 agcggtacgc attaagccta gaccaagcat cgtaacaatt aattccacaa tcacgtccaa
     1141 tggcattctt ggtccatcaa tgccaacaag ccattgcaac caaggattaa taacaaatac
     1201 gttggctaac cctaatccac aagtccagcc tataaaaggt cgccaccctg ctaaataaat
     1261 ggagcgccct ttatcatgca agagattaag caaaaattgc aattctttag ggtgttgttt
     1321 taatttatct ataacagttt gtgctttttg tttttcttct tcaattggga aaactttatc
     1381 tatgatcgtg cctacagctt ctatggaatt tgcttttatc gcggatgaaa aaatcttgag
     1441 tagtttcatt aataccccca aatccaaggc cgtggaaatc catcttcttt ggtcaaatca
     1501 tcgatgtgaa caaaacgaac cgattgcggt cctttttgac ttataccaaa gcctgtaaaa
     1561 ccaaaacttg gagcattttg gataagttta aaagcgtttt ccccacagat agaaatatcc
     1621 actgctctcc ctagggtatg tgggccgctc tgacttgttt tgctaacggt gctattatgc
     1681 ttagcacacc gaaatccaga agttatggga agcggaaaat gtaaatgttc acgcagtgcg
     1741 ataagtttat tcatgaaatt gttattcatt tcaactttgg cacagccgca gcgacacgcc
     1801 atttctttct cagaaaagaa gcgccaactc ataacttact tgtaagtagc ttatcaactt
     1861 tgctttcaag tcgatttaag gaagcttgca taacagtatc taatctctgc acttcgtcgt
     1921 cttgcttctt atcacgatgt aactgcatgg ctttaatttg aatttgtgct tcataaaacg
     1981 ctttaagttc aatttctaaa accgcaatcc gtttttcaat ttgtgctgcc caccaaatga
     2041 ctgatacagc tataacaagt gcggttaata aattgcttaa atttatgttt ttatcaaaca
     2101 cccagccttt acttattttt gccataataa aaccatcttt ttttaagatt actgaggata
     2161 cctaaacggg ataaacctaa taaataacaa aaatcgcgaa attgattcga caagaaccac
     2221 tgtcttgcaa tttttctttc gcgcgaatca aatggtttgt tagcatcatc ccaacattgt
     2281 tgaataattg cgtaatgaag ctgaccttcc ggtaattcgc taatttcttg tcgaggtgac
     2341 caaggaaatg aaggatagaa aatttccaaa atcaagaagt ggtattgata gaattttgca
     2401 ttctgaaaat atcatagcta attttttata gttgtacaag gaaattaaag tttatttaca
     2461 atatgggcaa tagctattgc ttcccaatcg gtaacaatca tagaaacttc atggtatttg
     2521 tgtgcccaac attttttcca attgga
//
LOCUS       DASTIB010000002         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_298071, whole genome shotgun sequence.
ACCESSION   DASTIB010000002 DASTIB010000000
VERSION     DASTIB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298071"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFP93_00030"
     CDS             <1..434
                     /locus_tag="VFP93_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843025.1"
                     /translation="ITQPAEGGVGMAQAMINAIQDAKIDPQLIQYINAHGTSTRINDA
                     YETMAIKLALGEHAYKTAISSTKSMTGHLLGAAGAVEVIFSALSIRDKIVPPTINHHA
                     KDDDCDLDYVPNYAREMDIEASMSNSFGFGGTNGALILRRV"
     gene            440..1243
                     /gene="pabC"
                     /locus_tag="VFP93_00035"
     CDS             440..1243
                     /gene="pabC"
                     /locus_tag="VFP93_00035"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HET9843026.1"
                     /translation="MNGKPSTCLPVSDRGLHYGDGVFETIRIPNKNLPLEVHPLWKLH
                     IRRLKKGCERLDLCFPGEELLAQEVEQIRLPNICCVIKIILTRAGNQFGYSPNTNATN
                     RIVMITHRGSMHTPSEFHIARSKINLAKQPFLAGIKHLNRLENVLAAKEINASFQEVI
                     LCNEQDEAIEGSRTNIFALNEENVWLTPALENGGVEGVLRTWILQHAAMLNISCEIAS
                     IPYAKLCQARAIFLTNSVIGIVPVARIQDMAKSCHPVKPIQRLWSSAWQ"
     gene            1234..>2035
                     /gene="mltG"
                     /locus_tag="VFP93_00040"
     CDS             1234..>2035
                     /gene="mltG"
                     /locus_tag="VFP93_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9843027.1"
                     /translation="MAVIRRLFIFFCFLSIFVGLFISYQVYAYYCKPIVLVEDVRFRI
                     EPGSSLRKILKELSSHQDIKPFSKKWYEIFIRMHANTTKIKAGEYILKKNMTGSELIS
                     QLIKGKVVLHSLTLIEGWTFKDCIKAIHKHPAIKKTFDTDDYTQIREKLIITESSIEG
                     FFFPETYYFPYGTTDRDFLLRAHQLMQKKLQLAWHARSPNLAIDSPYEALILASILEK
                     ESALVAEWPIISQVFHNRLKIKMRLQADPTVIYGLGANFAGRLNKDALK"
BASE COUNT          658 a          372 c          396 g          609 t
ORIGIN      
        1 atatcactca acctgccgaa ggtggtgttg gtatggctca agctatgata aatgcgattc
       61 aagatgcaaa aattgatccg caactaattc aatatattaa tgcacatggc acttccactc
      121 gaattaatga cgcttatgaa acaatggcaa ttaagctggc tttgggtgag catgcctata
      181 aaacggctat tagttctacc aaatctatga ctggtcactt attaggagcc gcaggtgctg
      241 tagaagtaat tttttcagcg ctaagtattc gcgataaaat tgtgcctcca acaatcaatc
      301 atcatgctaa agatgacgat tgcgatttag actatgtacc caattatgct cgtgagatgg
      361 atattgaagc aagcatgtcc aattcgtttg gttttggtgg taccaatgga gcgcttatat
      421 tacgtcgggt ttagcaatca ttaatggtaa accctcgact tgtttgccag taagtgatcg
      481 tggattgcac tatggtgatg gtgtattcga gacaatccgt atcccaaata aaaatttgcc
      541 tcttgaagtc catcctcttt ggaagttaca tatacgaaga ttaaaaaaag gatgtgaaag
      601 attagatctt tgcttcccag gtgaagagct gttagcacaa gaagtagaac aaatacgttt
      661 gcccaatatt tgctgtgtta taaaaattat tttaacaaga gcagggaatc agtttggtta
      721 ttcaccaaat acaaatgcga caaatcgaat tgtgatgatt acgcataggg gatcaatgca
      781 tactccaagt gaattccaca tagctcgtag caaaattaac ttagccaaac aaccttttct
      841 ggcaggtata aaacacctaa atcgtttaga aaacgtttta gccgcgaaag agataaacgc
      901 ttccttccaa gaagtgattt tatgtaatga acaagatgaa gctattgaag gcagcagaac
      961 taacattttt gcacttaacg aagaaaatgt ttggttaaca cctgctttag agaatggggg
     1021 agtggaaggt gtattgagaa cctggatact gcagcacgca gcaatgctaa acatttcttg
     1081 tgaaattgct tctataccgt atgcaaaact ttgccaagcg cgcgccatct ttttaaccaa
     1141 cagtgtaata ggcatagtgc cagtcgctcg catccaagat atggcgaaaa gttgtcatcc
     1201 cgttaaaccc attcaacgtc tgtggtcaag tgcatggcag taattcgaag actttttatt
     1261 tttttctgtt ttttaagcat ttttgtagga ttatttattt cctatcaggt ctatgcttat
     1321 tactgtaaac ccattgtctt agtagaagat gttcggttta gaattgaacc tggaagttct
     1381 ttaagaaaaa tattaaaaga attatcctcc catcaagata taaaaccttt ttcaaaaaaa
     1441 tggtatgaaa tttttattcg catgcacgct aatacgacaa aaattaaagc gggtgaatac
     1501 atactaaaaa aaaatatgac aggctctgaa cttatttcac agcttataaa aggcaaagtt
     1561 gttttgcatt ctttaacgct gatagaagga tggactttta aggattgtat aaaagcgatt
     1621 cacaagcacc cggccattaa aaaaactttt gataccgatg attatacgca aattcgtgaa
     1681 aaattaatta ttacagaatc ttccatagaa ggctttttct ttccggaaac ttactatttt
     1741 ccctatggca caaccgatcg tgacttctta ctacgtgcgc atcaacttat gcaaaagaaa
     1801 ttacaattag cctggcatgc gagatcaccc aatcttgcga tagatagccc ctatgaggca
     1861 ttgattttag cttctatctt ggaaaaggaa tcggcgctag ttgcggaatg gcccattatt
     1921 tcacaagttt tccataatcg tcttaaaata aaaatgcggt tacaagcaga tcccacggta
     1981 atttacggtt tgggcgctaa ttttgcaggg cgcttaaaca aagatgcatt aaaaa
//
LOCUS       DASTIB010000003         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_580903, whole genome shotgun sequence.
ACCESSION   DASTIB010000003 DASTIB010000000
VERSION     DASTIB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580903"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2115)
                     /locus_tag="VFP93_00045"
     CDS             complement(127..2115)
                     /locus_tag="VFP93_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843028.1"
                     /translation="MLNSIFPALSPNHNQGPSLFAAIRDGNEDIVRQSLNPSSKPLLE
                     DICEEGAQTNLLILGMHSSVIVAALLEAYPKISAFDKRDALLHAVDKDHIQVMIELLT
                     RCDDEISTNDKGMALQDAAKNGHSQAIFEILTRCGDKIDAFDKGSALMNAVRNGHPQS
                     IVTLLNGCGGEISNEDKLGALMAAIFKGHIDTVHSLLANRSVEAVATYNNNAALTEAQ
                     KRANGDEEDRARYQPIVDRLMQIPAVATLAAQQVQHATNLAHITQFGENSMQALNAEE
                     SSIVSHLKNHYKSMYEDKGFEVIRAEILSYLEESYKKSPALDNGHPLPLEYTVNLSNS
                     ALKAYYEHLTHGAWRYLSDNNPWMAANAEYIHTFEDGRKVAIISDADKELMSYLWIAL
                     SDETIQLDNGFMVEGNKNVFAQNLGELNRAHNHDERAANPLEQQAYKNKVPGIQITTV
                     DDLEADKPTCVYGVTKRLLESPYGHPRIKPLDPRLMQAWMESLLFGRKQKEEDLDNIV
                     DKLDKLSLHELKNIQENLIDALSGEAFECHNTLQIAQDQLNAFIERVKNWYSDEEITK
                     ERNPKLVINPAGSGFIAKEHNSYVDLIQFCTNDPINCFAQVLNTYLTARIPALEELEK
                     HDAEMENVNTFTLSYYDSSKKRERDEELKVQEKCRRLR"
BASE COUNT          678 a          473 c          416 g          779 t
ORIGIN      
        1 tcccgttttt gcgatgcgct cgcgggaatg acggaattga tatacggatt ttcataatta
       61 gtcattcccg tgcaagacac ggatccatta tatgatccgg gatcactttc gcgaggctcg
      121 cgggatttat cgcagacgcc tgcacttttc ttggacttta agttcttcat cgcgctctct
      181 ttttttagag gaatcgtagt aactaagtgt gaaagtattt acattttcca tttctgcatc
      241 gtgcttttct aattcctcta atgctggaat acgcgctgtt agataagtat ttaatacttg
      301 cgcaaaacaa tttattggat catttgtaca aaactggatg agatcaacat agctattatg
      361 ttctttagcg atgaaaccag agcctgcagg atttatgact aacttgggat tacgttcctt
      421 agtaatttcc tcatcactat accaattttt aaccctttca ataaatgcat taagctgatc
      481 ttgggctatt tgcagagtat tatgacattc aaatgcttca ccacttagtg catcaataag
      541 gttttcctga atgtttttta attcatgcaa agataattta tcaagcttat ccacaatatt
      601 atctagatct tcttcctttt gttttcttcc aaaaagtaaa gactccatcc aagcttgcat
      661 taatcttgga tctaaaggtt tgatacgtgg atggccataa ggactttcta gcagtcgctt
      721 agtcacgcca tatacgcagg taggtttatc cgcttctaag tcatctactg ttgttatctg
      781 aataccaggc actttatttt tatacgcttg ttgctcgaga ggatttgctg ctctctcatc
      841 atgattatgc gcgcgattta attcacctaa gttttgcgca aacacatttt tgttaccttc
      901 aaccataaaa ccattatcta gttgtatagt ttcatcactg agcgcaatcc aaagataact
      961 catcaattct ttatcagcat ccgaaataat ggcaactttt ctaccatctt cgaaggtatg
     1021 aatatattca gcatttgctg ccatccatgg attattatca cttaaataac gccaagcacc
     1081 atgtgttaaa tgttcataat aagctttgag cgcactatta cttaaattga cagtgtattc
     1141 taatggaagt ggatgtccat tatctaaagc aggggatttc ttgtaagatt cttcaagata
     1201 gcttaaaatt tcagctcgaa taacttcgaa gcctttgtcc tcatacatgg atttgtaatg
     1261 gttttttaaa tgcgaaacta tactggactc ctccgcattt aaagcttgca tcgaattttc
     1321 accaaattgc gtaatatgcg ctaggtttgt tgcgtgctgt acctgttgag cagccaaggt
     1381 agctacggct ggaatttgca ttaatctatc tactatcggt tgataacggg ctctgtcttc
     1441 ttcatcacca ttcgctctct tttgagcctc cgtaagtgct gcattattgt tataagttgc
     1501 aacagcttcc acgctacgat ttgctaacaa actatgcaca gtatcgatat gtcccttgaa
     1561 aatagccgcc ataagtgctc ccaatttatc ttcattgctt atctcaccac cgcaaccatt
     1621 gagaagtgta acgatagact gaggatggcc atttctaacg gcattcataa gggccgagcc
     1681 tttatcaaaa gcgtctatct tatcaccaca acgagtgaga atttcaaaga tagcttgaga
     1741 atgaccattt ttagcggcgt cttgaagcgc cataccttta tcatttgtac ttatttcatc
     1801 atcacaacgc gtgagaagct caatcataac ttgaatatgg tccttatcaa cagcatgtaa
     1861 gagcgcgtca cgcttatcaa aagcactgat tttaggataa gcttcaagca gtgcagcgac
     1921 aataaccgac gagtgcatgc ctaatattaa taaattagtt tgagcacctt cctcacaaat
     1981 atcttctaaa agaggttttg aagaaggatt aaggctttga cgtacgatat cttcatttcc
     2041 gtctctaatg gccgcaaata gtgatggacc ctgattatgg ttagggctca gggctggaaa
     2101 aatactgttt aacataaatt acctaaatac ataaagttac tacacttaaa aagaagaaaa
     2161 aatattcctt gcattcgatc taaagatgag cgctaccatt tattcactgt agatattaca
     2221 ttttcgttgg atcgcaatgt gtaaattttg ctctttttat aattttgttg aaatgaaaag
     2281 agtttgaagt attttaaaag agaaaggatg gccagctttt actggcaaca tccaaatcaa
     2341 taattt
//
LOCUS       DASTIB010000004         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1042775, whole genome shotgun sequence.
ACCESSION   DASTIB010000004 DASTIB010000000
VERSION     DASTIB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042775"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFP93_00050"
     CDS             <1..860
                     /locus_tag="VFP93_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9843029.1"
                     /translation="FTINETAVQVSSSAQETQSTATHLAQASESQAREIVGATAAINA
                     MAQSIEHVSSNAAKSALVADESVNIAKSGVQVVQNTMIGMEKIREQMQETSKQIKRLG
                     ESTQEIGNIIALINDISDQTNILALNASIQAAMAGEAGRGFAVVAEEVQHLAERATHA
                     TKQIETLVHTIQNDTNETVKSMEQTTQEVVIGAKLAKDAGAALGKIESVSFYLAELIS
                     KISEAAQLQAQTSSHVSTTMEIIKDITTQTAGGTTATANSIGNLAELAIALRDSVAGF
                     KLPIENDVA"
     gene            864..3503
                     /locus_tag="VFP93_00055"
     CDS             864..3503
                     /locus_tag="VFP93_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9843030.1"
                     /translation="MQNPQKEDLPTSEDLVNFNHAAHVQTEYIITNLELWRTDPTQLH
                     LLQLASKSVQTLANFAVQLKLIVINNLLKSIEKLLTALLQNTVAHEPVYKYIRMALNE
                     LEHLLEGVKKQEPLQENPELLAFYHQILEKIPQEDAPKIQAIEIIEPAVPIEIFHEEF
                     MEILQELEQQIQHYAQDKTLDVVSDFKRLLHTLKGSSRLVGFSHLGKFVHAIEDLVEE
                     KDVEESRALIQDTIRLLKQQEQKILEKINFNIDVEDLHQMQPLATNLPSPGVSTSSGG
                     ELEEKIRIPLHTLERLSQFAASTNIARAHIEQQQQLIKFYLNRIEDTLRHLRETSRAL
                     QLQSDLISLPSSSTTTREDFDPLELDRYTSLQQEAREIQGDVGNLFALYDNLSETLHS
                     LNERVREQRHSVQALEENLTHMRMITFESIVPRLEKIVESISKELKKDVRLKIVQLEG
                     EMDRTILQKLISPFEHMLRNAIDHGIESLEIRKALNKPLQGTIELTLIRKGPHVILQF
                     KDDGRGIDLDAVKKRALAEGWWKNTVEPSERDLLKLIFLPGFSTSKTLTTISGRGIGM
                     DIVNHEIRKIGGSLRIDTKKNEGTLFTIQLPFTLSINQALIFKGGFQLYGLLLSNLAG
                     ITRSSIEELFNLYTNKLSFHYMGKIFNLKYIGDFTGQGRLDPVNFLQKSLPVLLVEAQ
                     NQRLAIIIDSVVGPREVLVKSLGPQMQTVDEYLGASVLGDGKIVLMLDPFALVGSQVE
                     SVMSTEKTLYKSKLPMILVVDDSVTVRKVTTHLLERNQYQALSAKDGLDALEVLQNHN
                     PDCILLDLEMPRMDGFAFLENIQKKGGKNWPIIVISSRSTEKYKKRVFALDADEFLSK
                     PFQNDHLLATIHRLVKKYKQKNG"
BASE COUNT         1202 a          648 c          706 g          960 t
ORIGIN      
        1 tgtttactat caatgaaacc gcagtacagg tttcatcctc tgcccaagaa acacaatcta
       61 ccgctacgca tttagcccaa gcttcagaat cgcaagcgcg ggaaatagtc ggtgctactg
      121 ctgcaatcaa tgcaatggct caatccatag aacacgtttc ttccaatgca gccaaatccg
      181 cattagtcgc cgatgaatcc gttaatattg cgaaaagcgg tgtacaagtt gtgcaaaata
      241 cgatgatagg tatggaaaaa attcgagagc aaatgcaaga aacctcgaaa cagattaaaa
      301 gacttggaga aagtacccaa gaaattggta atattatagc gctcattaat gatatatcgg
      361 atcaaaccaa cattttggcc ttaaacgctt ccattcaagc agccatggca ggggaggcag
      421 gcagaggttt tgctgtagtt gccgaggaag ttcaacatct ggcagagcgt gcaacccatg
      481 caaccaaaca aattgaaact ttagtacata ccatacaaaa tgatacgaat gaaaccgtaa
      541 aatctatgga gcaaactacc caagaagtag tcatcggtgc aaaacttgcc aaagatgctg
      601 gtgcagcatt aggaaaaatt gaaagtgttt cgttttatct ggctgaacta attagtaaaa
      661 tttcggaagc tgcacaatta caagcacaaa cttcttcaca tgtatcaacc acgatggaaa
      721 tcattaaaga tatcacaacc caaactgcag gtggtacaac cgcaaccgca aactcgatcg
      781 gtaatttagc tgaactagct atagcgttac gtgactctgt cgcaggcttt aaattaccca
      841 tagaaaacga cgtggcataa cctatgcaga atccgcaaaa agaagatttg ccgacttcag
      901 aagatttggt aaattttaac catgcggcac atgtccaaac ggaatatatt ataaccaact
      961 tagagctttg gcgcaccgat ccaacgcaat tgcatttgtt acagcttgcc agtaaaagtg
     1021 tgcaaacgct tgcaaacttt gccgttcaat taaaattaat agttataaat aatttattaa
     1081 aatctattga aaaattatta acggctcttt tgcaaaatac cgtagcgcat gagcccgttt
     1141 ataagtatat tcgcatggca ttaaacgaat tagaacattt gctagaaggt gtaaaaaaac
     1201 aagaaccatt gcaggaaaat ccagaattat tagcatttta tcatcagata ttagagaaga
     1261 tccctcaaga ggatgcgcca aaaatacagg cgatagaaat tatagagcca gcggttccca
     1321 ttgagatctt tcatgaagaa ttcatggaaa tattgcaaga gttggagcaa caaatacaac
     1381 actatgcaca ggataaaacg ttagatgtag tatccgattt taaaagattg ctacatacct
     1441 taaaaggaag ttcgcgctta gttggctttt cgcatttagg taagtttgtg catgcaatag
     1501 aagatctagt tgaggaaaag gacgttgaag aatcgcgtgc tctgatccaa gatactattc
     1561 ggctcttgaa acaacaagag caaaaaattt tagaaaaaat taattttaat attgatgttg
     1621 aagatttaca tcagatgcaa ccacttgcga caaatctacc atcaccaggg gtgagtacgt
     1681 cttctggtgg cgagctagaa gaaaaaatac gaattccgct tcatacttta gaacgcctga
     1741 gccaattcgc tgccagtacc aatatagcgc gcgcgcacat cgaacaacaa caacaattaa
     1801 ttaaatttta tttaaataga attgaagata ccctccgaca tttgcgagaa acgagcagag
     1861 cgttgcagct gcaatccgat ctaataagtc taccctcttc ttcaaccaca acacgcgaag
     1921 attttgatcc attggaatta gatcgctata caagcttaca acaagaagcc cgggaaattc
     1981 agggtgatgt aggtaaccta tttgctttat acgataatct ttcagaaaca ttacacagtc
     2041 taaatgaacg cgtacgtgaa caacggcatt cggtgcaagc gcttgaagaa aatttaacac
     2101 atatgcgtat gattactttt gaatcgattg tacctaggtt agaaaaaata gttgaaagta
     2161 ttagcaagga attaaaaaag gacgtacgtt taaagattgt acaactcgaa ggagaaatgg
     2221 atcgtactat cttacaaaaa ctgatatcac cctttgagca tatgttgcgt aatgccattg
     2281 atcatggcat agaatctttg gaaattcgca aagcattgaa taaaccgtta caaggtacta
     2341 ttgaattaac gctaattcga aaaggtccgc atgtaatatt acaatttaaa gatgatggga
     2401 ggggcataga cttagatgcg gtgaaaaaac gcgctcttgc agaaggatgg tggaaaaata
     2461 ccgtggagcc cagtgaaaga gatttgctta aattaatttt tcttcctgga ttctcaactt
     2521 cgaaaaccct taccaccatt tcagggcgtg gcataggcat ggatattgta aatcatgaaa
     2581 ttcgcaaaat cggtggaagt ttgcgtattg ataccaaaaa aaatgaaggc actttattta
     2641 ccattcaatt gccgtttact ttatctatta atcaggccct tatttttaaa ggcggatttc
     2701 aattgtatgg cttattattg tctaatctgg ccggtataac gcgatcttcc attgaagagc
     2761 tatttaacct ttataccaat aaactatcgt ttcattatat gggtaaaata tttaacttga
     2821 agtatattgg cgattttact ggccaaggca gactagatcc ggtcaatttt ttgcaaaaga
     2881 gtttgcctgt gttgttggtg gaagcgcaaa accagcgtct tgctattatt atcgatagtg
     2941 ttgtgggacc tcgtgaggta ctcgtaaaat ccttagggcc acagatgcaa accgttgatg
     3001 aatatcttgg cgcaagtgtt ttgggggatg gcaaaattgt acttatgttg gatccttttg
     3061 cactggtggg atcgcaagtc gagtctgtaa tgagtacaga aaaaaccctc tataaaagta
     3121 agctaccaat gattttggtg gtagatgatt ccgtcacggt gagaaaagtt accacccact
     3181 tattagaacg taatcagtat caagcgttaa gtgcaaaaga tgggctcgat gcgttggaag
     3241 tgctgcaaaa tcataatcca gattgtattt tgcttgattt agaaatgcct cgcatggatg
     3301 gttttgcttt tttagaaaac atacaaaaga agggaggaaa aaattggcca attatcgtca
     3361 tatcttcgcg atccacggaa aaatataaaa aacgtgtttt tgcacttgat gcagatgaat
     3421 ttctttcaaa accctttcaa aatgatcatt tattggcaac gatacatcgt ttagtaaaaa
     3481 agtataagca aaaaaatgga tgaaaaaaat atttac
//
LOCUS       DASTIB010000005         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1311438, whole genome shotgun sequence.
ACCESSION   DASTIB010000005 DASTIB010000000
VERSION     DASTIB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311438"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFP93_00060"
     CDS             complement(<1..216)
                     /locus_tag="VFP93_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9843031.1"
                     /translation="MRTHYCGTLTESHLDTKVHLYGWVHRRRDHGGVIFVDIRDKTGL
                     VQVVFHPENQEAFTKAERLRSEFVIAVE"
     gene            complement(257..502)
                     /locus_tag="VFP93_00065"
     CDS             complement(257..502)
                     /locus_tag="VFP93_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9843032.1"
                     /translation="MPIYEYSCTECHHQTEILQKAQEQISTTCPNCNQPSFTKAISAP
                     RFRLGGSGWYETDEKKKEKQRYIKNREETTSSPSESA"
     gene            1032..2696
                     /locus_tag="VFP93_00070"
     CDS             1032..2696
                     /locus_tag="VFP93_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9843033.1"
                     /translation="MRIKPMGLNHEAIVEESNSFIQSPIESQNWQALQIFSLFRFILA
                     STFFILSKFDLGLTFVNITNKTWFMTITEVYLALSFLSLIGCVFKKPDFDLQVNLPIF
                     FDILAITLLMHMSGGLLNGIGLLLIVIVAAHSLLAQSLLALCGASIASCAILTEQVYI
                     HFTHSKIPTAYSQAGMLGLSIFAIALVAIWLKRRLQNMQHLAYTRGLKLATSLQLNAQ
                     VIAFMQEGVVVFDNNFNIQLINGAACRLLGIPISQPMQHLMDLPVRFQAAFEAWKQQQ
                     SEEIFQIQKNTPEIRISGSVLSPSGTVMGTVIFIHDISKESQKAQHLKLALLGSLAAN
                     IAHEIRNPLSSVSHAAQLLNETYREDKENQSLVNIIKDNCDRMNIVIKNVLTISKKQN
                     ARPELINLNSFLKKFIEDLQPPGLPHVEINLSCFTKELFVFIDPTQLHQMLVNLCENG
                     LRYSMRYYNKPRLDIILEENLDLGEVEIHIIDFGHGIDGRDVKHVFEPFYTTEPHGSG
                     LGLYITKELCQMNGGEIHYQQAPTGGSDFSMTLPLYQETKQYDIVR"
     gene            2680..>3653
                     /locus_tag="VFP93_00075"
     CDS             2680..>3653
                     /locus_tag="VFP93_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9843034.1"
                     /translation="MTSFDNARSSIKKKPKALVIDDEPDICELIRISLDRMNIETIIA
                     HNYHQALRLLQDNHYQLCFTDMRLPDGDGLDLLNYIQKNIPHLPVAVITAHGTMDSAI
                     TALKRGAFDFVSKPIDLEMLRGLAQTALRLEEPSNTSEVNILGNSKVTQELREKISKV
                     SRSQAPVYISGPSGSGKELVARVIHQLSPRSDQPFVPVNCGAIPKELMESEFFGHLRG
                     SFTGAHQDKKGLFQAAHKGTLLLDEVGDLPLEMQVKLLRAIQERAIRPIGSSKEVMVD
                     VRILSSTHKNLYELVKCNQFREDLFYRLNVIELKVPGLRERAEDIPQLT"
BASE COUNT         1111 a          709 c          705 g         1128 t
ORIGIN      
        1 ttccacagca ataacaaatt ctgagcgcaa acgttctgct ttggtaaaag cttcttgatt
       61 ttcagggtga aagactactt gtaccaggcc tgttttgtct cgaatatcga caaaaattac
      121 gcctccatga tctcttctcc gatgcaccca accatacaaa tgtacttttg tatctaaatg
      181 agactcagtt aaagtaccac agtaatgcgt acgcatagtc tttcctattg ttaaattcta
      241 ccgaatgctt aacggtttaa gcagattcac ttggggagga cgtcgtttct tcccgatttt
      301 ttatatacct ttgcttctct ttcttctttt cgtcggtttc gtaccaaccg ctacccccta
      361 atcgaaaccg aggtgcgctt atagctttcg tgaagctcgg ctggttacaa ttaggacaag
      421 tagtgctaat ttgctcttgg gctttttgca aaatttccgt ttgatgatgg cattccgtgc
      481 agctatattc gtaaattggc ataatttaac tcactataga tatccaacta aattcacata
      541 gcaagcaaat gcttacatat aaaaaaccct gattataata ctgcgcggcg gtattaagaa
      601 gaggtaagat aggacagcca acatagaatg cggttagaag ttgctaaacg gttactcgca
      661 acatagggag atagaggcac agggttacca aagccatcca aaaagctaaa catttgaaag
      721 ttttctaata tatatttaat ctcccttaca gttgaagcgt gtaaataata aagtctttga
      781 ggaaatcacg tgatccgttt catactacta tgtatcttat ttgcattttt gttacgacgc
      841 attggatggt tgatcaggtt gttttggaag cagaacaact accacaacgc gcataacacg
      901 cgatctaagg ataatacctt agttaaatgc acgcattgta acgtctatgt cgaaaagtcg
      961 aaaatttttg tccaacaaca caaacctttt tgttcgaaag agcacgcaag ccagcatcaa
     1021 aggcaaaatg catgcgtata aaacctatgg gtttaaatca tgaagccatc gtagaagagt
     1081 ctaactcttt tattcagtct cctattgaat cgcaaaattg gcaagcttta caaattttta
     1141 gtttgtttcg ttttatctta gcttcaactt tttttatttt atcgaagttt gatctgggtt
     1201 tgacgttcgt gaatattacg aacaaaacgt ggtttatgac tatcactgaa gtctatttag
     1261 ctttaagctt tttaagttta attggatgcg ttttcaaaaa acccgatttt gatttacagg
     1321 taaatctgcc tatttttttt gatattctag cgattacctt acttatgcat atgagtggtg
     1381 gcttgttaaa tggtattggt cttttactca ttgtcattgt agccgcccat agcttgttag
     1441 cccaaagttt attggcgcta tgtggtgctt ctatcgcatc gtgtgcaatt ttaacagaac
     1501 aagtttatat ccatttcacc cattcaaaga ttccaacagc ttattcacaa gcaggaatgc
     1561 taggccttag catttttgcc atagcgctcg tggctatttg gttaaaacga cgtttacaaa
     1621 atatgcaaca tttagcttat acccgagggt tgaaactggc gacctcttta caactcaatg
     1681 cccaagttat cgcgtttatg caagagggcg tggtggtatt tgataataat tttaacatcc
     1741 aattaatcaa tggtgctgct tgtaggcttt taggtatacc tatttcacag cctatgcaac
     1801 atcttatgga tttacccgtg cgctttcaag cggcctttga agcgtggaaa caacaacaaa
     1861 gcgaagaaat ttttcaaatt caaaaaaaca cgcccgaaat tcggatcagt ggatcggtgt
     1921 tgtccccctc tggtacagtc atgggtaccg tgatatttat tcacgatata tcgaaagaat
     1981 cgcaaaaagc gcaacattta aaattagcgc tcttaggaag cttggcggca aatattgccc
     2041 atgaaattcg caatccttta agttcggtgt cgcatgcggc acagttatta aatgaaacgt
     2101 atcgcgaaga caaggaaaat caaagcttag tcaatattat taaagataat tgtgacagaa
     2161 tgaatatcgt tataaaaaat gtattaacta tttccaagaa gcaaaatgca cgtccggaat
     2221 tgatcaattt aaacagcttt ttaaaaaaat ttattgaaga tttacaaccg cccggtttac
     2281 cccatgttga aatcaattta agttgcttta ctaaagaatt gtttgttttt attgatccaa
     2341 cccaattaca tcaaatgtta gttaatcttt gtgaaaacgg cttacgctat agtatgcgct
     2401 attataacaa gccgagatta gacattatct tagaagaaaa tttggatcta ggcgaagttg
     2461 aaattcatat tattgatttt gggcacggaa ttgacggtag ggatgtaaag catgtttttg
     2521 aaccctttta taccacagaa ccccatggtt ctggtttagg tctgtatatt accaaagagc
     2581 tgtgtcaaat gaatggcggt gaaattcatt accagcaagc acccaccggc ggtagtgatt
     2641 ttagtatgac tttgccgttg tatcaggaga caaaacagta tgacatcgtt cgataacgcg
     2701 cgatcttcga tcaaaaaaaa acccaaagca ttggtcatag atgatgaacc tgatatatgc
     2761 gaactgatac gcatctccct cgatagaatg aatattgaga ctattattgc ccataattat
     2821 catcaagctt tacggttgtt gcaagacaat cattaccaac tttgctttac cgatatgcgt
     2881 ttaccggatg gggatggatt agatttactc aattatattc aaaaaaatat tccccatctt
     2941 cctgttgcgg taattacagc gcatggcaca atggattctg ccattaccgc gctaaaacgt
     3001 ggcgcttttg attttgtaag caaacctatt gatctggaaa tgcttcgagg attggcacaa
     3061 acagctttgc gtttagaaga accttccaat acatccgaag taaatatttt aggaaattcc
     3121 aaagtaacac aagaacttcg ggaaaaaatt agtaaagtat cgcgatcgca ggcgcccgtt
     3181 tacatcagtg gaccttcagg atcggggaaa gaattggtag cgcgtgttat tcaccaattg
     3241 agcccgcgat ccgatcaacc ctttgtgccg gttaattgtg gtgctattcc caaagagctc
     3301 atggaaagtg aattttttgg tcacctacgt ggcagtttta ccggtgcgca tcaagataaa
     3361 aaagggttgt ttcaagccgc ccataaaggc acattattgc tagatgaagt tggggatcta
     3421 ccgctagaaa tgcaagtaaa attattacgc gccatccaag aacgtgccat acgtcctatt
     3481 gggtcatcta aagaagtcat ggtagatgta agaattttat cttccaccca caaaaatctg
     3541 tatgaattag ttaagtgcaa ccaatttcgc gaagaccttt tttaccgttt aaatgttatc
     3601 gaattaaaag tgcccggttt acgcgaacgt gcagaagata ttccacagct aac
//
LOCUS       DASTIB010000006         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1311299, whole genome shotgun sequence.
ACCESSION   DASTIB010000006 DASTIB010000000
VERSION     DASTIB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311299"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..919)
                     /locus_tag="VFP93_00080"
     CDS             complement(221..919)
                     /locus_tag="VFP93_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843035.1"
                     /translation="MLEFHEKCVDFFKAFKKALALEDKMLKAARGKTKNLSVSLVEIE
                     QNNLQHILEKTENPDLTSPAYLEKILDYLKRCEEVLRRKQQTMQLNAAFVPIPIHQKL
                     NQEYSQLLQQLQEILLLSPVQKDAKKSWLDTRLRAGFYYRRLTIDHVGLKNAANKAHA
                     EEDLQALQLELQQKASYPVPTKTTQRLLEDEKHNRNFPNDFYPALFAFRKTSSEKITA
                     DALEKILQQPAPSV"
     gene            complement(922..1230)
                     /gene="hspQ"
                     /locus_tag="VFP93_00085"
     CDS             complement(922..1230)
                     /gene="hspQ"
                     /locus_tag="VFP93_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HET9843036.1"
                     /translation="MEAKFEIGQVIHHLTYDYRGVVVDVDPIFYGTNDLYDQLAQNQP
                     PKDKPWYHILVDNTTLMTYASEKQIEPDISGIPIEHPWVGHFFDGFNNGKYHKRQKDN
                     "
     gene            1411..1983
                     /locus_tag="VFP93_00090"
     CDS             1411..1983
                     /locus_tag="VFP93_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843037.1"
                     /translation="MILKKISLLFGCAILTASAYAQYDFGYEVGAGHYNLNGVTKTRP
                     SNNFKVGFTDDASKLGVDAIYNMVPKTSYGPSSAHVDQRVQGVKLLAKADLDIIERFT
                     ATVRGGLGYEHVRSHMSSRVNSEVTSSSSSRTDEYFPSVAAGLNFDATQQMQLGLKVN
                     HDARKDYKFKDNTYVTLGFTYLLPSGKTLA"
BASE COUNT          642 a          378 c          382 g          727 t
ORIGIN      
        1 catacgtttt gtgcctattg tacgtataac ccggggtaaa aaatcacaaa ggctggattt
       61 cttaaaattt gattaattag ttaccgtact atctaatagt aagaaatgct aatcataaca
      121 aaataatact agaattctca agcaaaatat taaataaatt aatttatccc cagatcctac
      181 agtaagtatg tgcgagcatg tcacacatac ttatgaagta ttatacggaa ggtgctggtt
      241 gctgcagaat cttttccaat gcgtcagcgg taattttttc tgaagaagtt tttcgaaatg
      301 caaataaggc aggataaaaa tcgttcggaa aattcctatt atgcttttca tcttcaagaa
      361 gtctttgcgt agtttttgtt ggaacaggat aggaggcctt ctgttgtaat tctagttgca
      421 atgcttgcaa atcttcttct gcgtgtgctt tattagctgc attttttaag ccaacatgat
      481 caatagttaa gcgtctataa taaaagccag ctcttaatct agtatcgagc caagatttct
      541 tcgcatcctt ttgtacagga gataaaagta aaatttcttg taattgttgt aaaagttgcg
      601 aatattcttg gtttagcttt tggtgaatag gtatgggtac gaaagcagca ttcaattgca
      661 tcgtttgttg tttgcgtcgt aatacttctt cacaacgttt taaataatct agaatttttt
      721 ccaagtaagc aggtgatgtg agatcgggat tctccgtttt ttctaaaata tgctgtaaat
      781 tattctgttc aatttccaca agggaaacac ttaaattttt cgtttttcct cgggctgctt
      841 taagcatttt atcttcgagg gctaacgcct tcttaaaagc tttaaaaaaa tcaacacatt
      901 tttcatggaa ttcaagcatt tttaattgtc cttttgtctt ttgtggtatt ttccattatt
      961 aaatccatca aaaaaatgac caacccaagg gtgctcgata gggatccctg aaatatcagg
     1021 ttctatctgt ttttcactgg cataggtcat gagcgtagta ttatctacta aaatatgata
     1081 ccagggttta tctttggggg gttgattttg cgcaagctga tcgtacaaat cgttagtccc
     1141 ataaaatata ggatccacgt ccaccaccac acctcgataa tcgtaagtca gatgatggat
     1201 aacttgacca atttcaaact tcgcttccat agagtactgc ttttctaccc aattttactt
     1261 tcataaatgg atgtcttcta tcaaagttta gcaaccaacc aaagatttgt tcttattttt
     1321 gtcctaggtt tactcctgat tcagttgcaa accatttgaa tttttattag gcttcgttcg
     1381 caaaatttat aataaaaatt tggagaatct atgattttaa aaaaaatatc tttattattc
     1441 ggatgcgcaa tattaaccgc aagtgcatat gctcaatacg attttggcta tgaagtgggt
     1501 gctgggcatt acaatttaaa tggcgttacc aaaacacgtc ctagtaataa ttttaaagtc
     1561 ggattcaccg atgatgcaag taaattaggc gtggatgcta tttataatat ggtccccaaa
     1621 acttcgtatg gaccttcctc tgcacatgtg gatcaacgtg ttcaaggtgt gaaacttttg
     1681 gccaaagcag acttagatat tattgaacgc ttcacagcga ccgtacgtgg cggcttgggt
     1741 tatgagcatg tacgcagcca catgtcttcg cgtgtaaatt cggaagttac atcttcctct
     1801 tcttcgagaa ccgatgaata ttttccaagt gttgcggctg gtttaaattt tgatgccact
     1861 caacaaatgc aacttggttt aaaagtaaac catgatgcgc gcaaagatta taaattcaaa
     1921 gacaatacgt atgtgacttt aggttttact tacttattac cttcaggcaa aaccttagcg
     1981 taatttttga gcatggatgc tccaaaaaaa attgcatacg actcgcaaat aatttagaat
     2041 tctttctaat ttcaataaaa gtataaaaga gcttatgcga aaccttacca ttgaagaata
     2101 ttcctttatt gctggcggat ttctctctg
//
LOCUS       DASTIB010000007         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_744674, whole genome shotgun sequence.
ACCESSION   DASTIB010000007 DASTIB010000000
VERSION     DASTIB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744674"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>2077)
                     /locus_tag="VFP93_00095"
     CDS             complement(139..>2077)
                     /locus_tag="VFP93_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843038.1"
                     /translation="PVETNKVEENKNPLINNNFSEQNEPNPATVVIPVPPPLPPKKVL
                     TPAVAKSNEKAQADVILNKGKAKLELAQIEAEETTLPEKSEVDKRVHFDLSNQDESED
                     DLRQGKQQQQANDTDDVERNGILRDLMDEIHHFNKDVLKKTPVDNEPKAQTPHDALMA
                     QIPAAAKQLKNVADPDSVRAPKIQAIEEGNPNRPLNPREELMKSIREQGKLNKAQDPE
                     DTKLKDTKVANDASSIEVSENAENKEKEKIALEHSATMDENPNNVNLEADRYHDAAKA
                     EPNQVVNTIPAPPPLPPKKGLTPAFSTPKNKLETEESARPNEVEVSIEGNKVKPNAAL
                     QKLKAMDESANARAATMEQRVNGVKLKKVDTPKPVALKTDATSAAEILKNKIEQQLPT
                     QHAETIEQKILTNKCSKVSQLVLEKLGQVDPDSKANYENKIKEQLPEEIKSVLTEDEF
                     FGVEEDNLVDNWNDAHTKAVVEKIATNLLQQEISELQHEVKTVPTAPATNVKYASIVD
                     KSISDALVKGVLGRRLQIEDESDSDDDMDMDDDFEDNSHRPVTQSPVNKLHDVQADLS
                     TKKSSVDSDAGTKDNAIKIHDVLHDGGVPALKASSDALVTSDVLSGQSDLVDSSMHAP
                     SPISHDIPGFELDYHQAQLHT"
BASE COUNT          473 a          427 c          385 g          792 t
ORIGIN      
        1 gcctatttat gaaattcaat taaatgacat tacgcaaccc tttagccctc ctataaccgt
       61 tgccgatata cgacttttat ttgaaggagg agaaggtggt ttggatgttt ttttcaaaaa
      121 catccaaatt ctcgactctt aagtatgcag ttgtgcttga tgataatcca gctcaaagcc
      181 cggaatatca tgggatatgg gacttggtgc atgcattgaa ctatccacta aatcactttg
      241 acccgacaat acatcgctag taactaaagc atcgctagag gcttttagtg ctggaacacc
      301 accatcgtgc aagacatcat gtattttaat tgcattgtct tttgtgccag catcagaatc
      361 tacagacgat ttcttggtcg acaagtcagc ttgaacatcg tgtaatttgt ttacaggact
      421 ttgtgtgacc gggcgatgag agttatcttc aaaatcatca tccatgtcca tgtcatcatc
      481 agaatcgctc tcatcttcta tttgcagtct tctgcctaaa acacctttta caagcgcatc
      541 actaatggac ttatccacta tacttgcata tttgacgttt gttgcaggtg cggtaggtac
      601 agttttaact tcatgctgta attcagatat ttcctgttgc aataaatttg ttgcaatttt
      661 ttctacaacg gcttttgtat gcgcatcatt ccaattgtct actaaattgt cttcttcaac
      721 accaaagaat tcgtcttcgg ttaataccga tttaatttct tctggtaatt gttcttttat
      781 tttattttca taatttgcct ttgagtctgg atcaacttgc cctaattttt ctaatactaa
      841 ttgtgaaact ttcgaacatt tattcgtcaa gattttttgt tctatggttt cggcatgttg
      901 ggttggcagc tgttgctcga tcttattctt tagtatctct gctgccgatg ttgcgtccgt
      961 tttcaaagct acaggttttg gcgtatccac tttttttagc ttaacaccat taactctctg
     1021 ttccatagtt gctgcgcgcg cattcgcact ttcgtccata gcttttaatt tttgcaatgc
     1081 ggcattaggc tttaccttat taccttctat gcttacttcc acttcattag gtcttgcact
     1141 ttcctctgtt tccagtttat tttttggggt agagaacgca ggcgttaacc cttttttagg
     1201 tggtaacggt ggtggtgctg gaattgtatt aaccacctgg ttaggttccg cctttgcagc
     1261 atcgtgatac ctatcagctt ctaaattaac gttatttgga ttttcgtcca tggtagcgct
     1321 atgttctaat gcaatttttt ccttttcttt attttcagca ttctcggaca cttcaatcga
     1381 tgacgcatca ttcgctactt tggtgtcttt taatttagta tcttcaggat cctgagcctt
     1441 gttaagcttg ccctgttcac gaattgattt catcaattct tcgcgcggat ttaatgggcg
     1501 attaggattg ccttcttcaa tggcttggat ttttggtgca cggacactgt ctggatctgc
     1561 aacattcttt aattgttttg ccgctgctgg aatttgtgcc atcaatgcat catgtggggt
     1621 ttgagctttc ggctcattgt ctacaggcgt cttttttaat acgtctttgt taaaatgatg
     1681 aatttcatcc attaaatctc ttaatattcc atttctttca acatcgtccg tatcgttcgc
     1741 ttgttgctgt tgctttcctt gacgcaaatc atcttcggat tcatcttgat tagataaatc
     1801 aaaatggaca cgcttatcaa cttctgattt ttcaggtaga gtagtttcct ctgcttcaat
     1861 ctgagccaat tctaattttg cttttccttt gtttaagatt acatcggctt gtgctttttc
     1921 attgcttttc gcaactgcag gcgttaacac tttcttaggt ggcaatggtg gtggaacagg
     1981 aatcacaact gttgcaggat tgggttcatt ctgctccgag aaattattgt taataagagg
     2041 atttttattc tcttcaactt tatttgtttc gacgggt
//
LOCUS       DASTIB010000008         6191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_387202, whole genome shotgun sequence.
ACCESSION   DASTIB010000008 DASTIB010000000
VERSION     DASTIB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387202"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VFP93_00100"
     CDS             <1..1233
                     /locus_tag="VFP93_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843039.1"
                     /translation="EIAKDFYRIVARDLVSKGFTHKVNCGSLSGISREVGVGEKLGIA
                     EEKFIDYAGYSDSSSQLVLFDKITPFYWYGQDHWYWYGKDTQYTKLKNALFKEELILS
                     EHLANSKLLGHVINWALLHNRKDILDGLQEIAQTVDRSDELHVLINSMKQFIDSPNNF
                     EVEKFVLNNQYYVDTVNSNGNTLLMQACFKGNDKLVYLLSEKGTDVNLINKSGETALH
                     IAASSKTIDKGVITKLIEKGAKINTEDNFGATPLMRAIINKNGTHKVQELLARGADVK
                     AKDHYGNAALMYAAKCGNSAIIPALVQRGAYIDDCDFSGDTPLITAIKKDDVACVVEL
                     INNGADVTMQDNVPLTWAECMDNAEIINIIKEKIYEVVKRKTLLFSNNMQPNRAYLSP
                     LDAHDEEDNTRVAEFQIE"
     gene            1663..2172
                     /locus_tag="VFP93_00105"
     CDS             1663..2172
                     /locus_tag="VFP93_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9843040.1"
                     /translation="MKTGILLTTALAICVSLNTAVAKSGEKEQNKAHVELTPEELNWI
                     KGPDSLPSGVQMVILEGHPSKAGSFTMRLKVPAHYKIPAHWHPADEHVTVLQGSMYMG
                     MGDKLDENQGKKLPIGSFAAIPKKMHHFAWTGGEETIIQLHGNGPWGIHYLNPNDDPR
                     KNKNNQESS"
     gene            complement(2358..3074)
                     /locus_tag="VFP93_00110"
     CDS             complement(2358..3074)
                     /locus_tag="VFP93_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843041.1"
                     /translation="MTQYSPAWAHGDLQEIFPNVFFVMGTNITSHEGIELQHSCNMVV
                     VKSGNALTLINTIRLNNDALSALDNLGQVTNVVRIGAFHGRHDAFYIDRYSAKLWALK
                     GVQHDNNKSTDVELIPHGEMPFPDCSVFVFETSIHPEAILQVNREGGILITCDSVKNW
                     TCIDPFFSEQTGKLYEKLGFLGEATISKIWQQACNIQAHDFARLKLLHFKHLLSAHGE
                     PLLHNAYETLSATIEQEFKI"
     gene            3264..3818
                     /locus_tag="VFP93_00115"
     CDS             3264..3818
                     /locus_tag="VFP93_00115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET9843042.1"
                     /translation="MAIKKIAVLIGSLRKGSYNRKVAQAVIKMAPSSLKLEIVEIGNL
                     PLYNEDDDNNPPESYVAFRELIKNFDGLLFVTPEYNRSIPGALKNALDVGSRPYGQSA
                     WQQKPAAIISVSIGAIGGFAANHHLRQALVFLDVPTMQQPEAYIGNAANLFEDNGEFK
                     NKKTNEFLEKFMINFATWVETNCK"
     gene            complement(3898..5730)
                     /locus_tag="VFP93_00120"
     CDS             complement(3898..5730)
                     /locus_tag="VFP93_00120"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HET9843043.1"
                     /translation="MLSYLKQLVDKAYKGILETIQSPFLKLQTLESRSAKDKSTLEAM
                     RSKAYKKIGEALKNPTFDYTEINREITQDTKAWINRVFTQELKALGKPPTQFSVLTLG
                     SLARQETGPITDLEIGFLIEKDSLEARKYFYTLAQNVSDRLYLLGEHPSIGGKGLRMD
                     EADNAPPHFKFFARNANFAQNKQLLTEAIKNRDWDKIPFEGSRPFLATPEQFADFTLE
                     KFPHNNLSKNEIKKRKKELEQLALNQALKKNPSIKNNSKAFKALRDEIQFYANEMFKP
                     YSPHEQNIARDAGRKLGRNTAFLFGDEGLYNKFKTRKESHLIKKNPKTGLSERQTIAY
                     EVMINDISQKYEKKSELFINGKLGKKIDLKRELYRFGEQFITNLGFYYNAPVQNTTEI
                     VKHLIKTKRVTEDFGAELIDYMNFATKMRLKKQMTLKHQSAIGVYIDKEAFEKDRKDL
                     EEKVEGLKLKLNYLQSIKANSQDIAKIERELNKEQINYEHLLEMAPGKIINPEDEKLL
                     REKYAPFLKKMFDEANIWTKNIMEKRDCPIGAGYTAPLAKMPKPVQTNNKPAVTFAKK
                     LQEKRDATIITPAAQQESNPSWVSKLYQHFIGQSAFNPVKAPRP"
     gene            complement(5848..>6191)
                     /locus_tag="VFP93_00125"
     CDS             complement(5848..>6191)
                     /locus_tag="VFP93_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843044.1"
                     /translation="DLEKQLMAPALLPLFALSLKRHLANTLIKRDIFNHYSPIIAKFC
                     AEKRLDYPTNAIAKFVEALAHYHAFEEHFEKNAFLESLDESFLIEQAPMIDVFVEHLE
                     NKKLPSYASTR"
BASE COUNT         1967 a         1060 c         1134 g         2030 t
ORIGIN      
        1 gaaatagcaa aagactttta ccgaattgtg gcaagagatc ttgtgtcaaa aggatttacc
       61 cataaagtca attgtggaag tctatctgga atttcccgtg aggtgggtgt aggtgaaaaa
      121 ttaggcatag cagaagaaaa atttattgat tatgcagggt acagtgattc ctcctcacaa
      181 cttgtgcttt tcgataaaat taccccattt tattggtatg ggcaggatca ttggtactgg
      241 tatggaaaag atactcaata taccaagttg aaaaatgcat tgtttaaaga agagttaata
      301 ttatctgaac atttggccaa ttctaagtta ttggggcacg tcataaattg ggctcttcta
      361 cataatcgaa aagatatttt agatggtttg caggaaattg cacaaaccgt ggatagatca
      421 gatgaattgc atgtattgat aaactccatg aaacagttta ttgattcgcc caataatttt
      481 gaagtagaaa agtttgtttt aaataatcaa tactatgtgg atactgtaaa tagtaatggc
      541 aacacactat tgatgcaagc atgttttaaa ggtaatgata agcttgtata tttactgagc
      601 gaaaaaggca cggacgttaa tttaataaat aaatcaggtg aaacagctct acacattgca
      661 gcatcttcga aaaccattga taagggtgtg ataacaaaac ttatcgaaaa gggcgcaaag
      721 atcaatacag aagataattt tggcgcaaca cctcttatgc gcgcaataat caataaaaat
      781 ggaacacata aagttcaaga actacttgca cgaggtgccg atgtgaaggc taaagaccat
      841 tatggcaacg cagctttgat gtatgccgca aaatgtggaa atagcgcaat tattccagct
      901 cttgtacaac gaggtgcata tatcgatgat tgtgatttta gtggcgacac gcctctgatc
      961 acagcgataa aaaaagacga tgttgcatgt gttgttgaac tcataaataa tggtgctgat
     1021 gtaactatgc aagataatgt accgcttacc tgggcagaat gtatggataa tgctgaaata
     1081 atcaatatca ttaaagaaaa gatttatgaa gtcgtaaaga gaaaaacgtt gttgttttca
     1141 aataatatgc aaccaaatag ggcttactta tctcctctag acgctcatga tgaagaagat
     1201 aacaccaggg ttgcggaatt ccaaattgaa tagaggtttg tagagaatta cagggatatt
     1261 ttttatacag atttgatttt atcataatca aaagaaagga aagctgtgct ttgcataaca
     1321 cttatgacaa cttttcttga ttgaatccaa tatagaagac aaagttgtca cctaactttg
     1381 ttttagtatc gagattaaaa aacgcaacaa actactgact cgcagtatcg ctaagttatt
     1441 ctttttaaaa acaaggtcta ggcacagtca aatctataat tcattaagtc tgagatgatt
     1501 gaccggcatt actaagtatt tcatacttga gagttctccg gatattttat gcagaaaaaa
     1561 gaaattttta gattcagatt gcgctctgca caattctaga aaacccaaaa atttaaaatt
     1621 gtgtaagttt tttaatttta tatttaatta aaggagatag gaatgaaaac gggcatttta
     1681 ctaaccactg cactagcaat atgtgtttca cttaataccg cagtcgcaaa atctggggag
     1741 aaggagcaga acaaagcgca tgtcgaatta acgcctgaag agctcaactg gattaaaggg
     1801 cctgattcat taccttcagg agtgcaaatg gtaattctag aaggacatcc atcaaaagca
     1861 ggctctttta ccatgcgtct taaagtgccg gctcattata aaattcccgc ccattggcat
     1921 cctgccgatg aacacgttac ggtgttacaa ggatctatgt atatgggaat gggcgataaa
     1981 ttggatgaaa atcaaggtaa gaaacttcca ataggcagct ttgctgctat tcccaagaaa
     2041 atgcatcact ttgcttggac aggtggcgaa gaaaccatta ttcaactaca tggcaatggt
     2101 ccatggggta ttcattatct taatcctaat gatgatccaa gaaaaaacaa aaataatcag
     2161 gaatcgtctt aaatgagcac atataggtga aaattttaaa aacgcgtatg ttatcgcggt
     2221 taatattagc tttctttttg acaatttcca cccaattttt ataggataac tgcgcctccc
     2281 ccttgaggcg cacccttggc ctgccatctt cttatggaaa ggaccatgca atggaaatca
     2341 taaaatggtt ataaaaatta aatcttaaat tcttgttcaa tagttgcaga taatgtttca
     2401 taagcgttat gaagcaaagg ttctccatgc gcactcagaa gatgtttaaa atgcaataat
     2461 tttaatcttg caaagtcgtg tgcttgaata ttacaagctt gttgccatat ttttgatata
     2521 gttgcttcgc ctaaaaatcc caatttttca tataattttc cagtctgttc actgaaaaat
     2581 gggtcgatac aagtccaatt tttaacacta tcgcacgtaa taagtatccc accttctcga
     2641 tttacctgta gaattgcttc aggatgtatt gaagtttcaa acacaaatac agaacaatct
     2701 ggaaatggca tttcaccatg agggataagt tcaacatcgg tgcttttgtt attatcgtgc
     2761 tgtactccct tcagagccca aagctttgct gaatatctat caatatagaa ggcatcgtgt
     2821 cttccatgaa atgctccgat tctaacaaca ttcgtaactt gtcctaaatt atctagagca
     2881 gacaaggcat cattgtttaa tcttatagtg tttataaggg ttagtgcatt gccacttttc
     2941 actacaacca tattacagct atgttgtaac tctatccctt catgggatgt aatatttgta
     3001 cccataacaa aaaacacatt gggaaaaatt tcttgaagat caccatgagc ccatgcagga
     3061 gaatattgag tcacttgtat accatccatc ttaaaattgg aagcaataat cccatatcta
     3121 aggaacgact tcaagattac ttaaatttca aacttaaccg tgttgcgatc ggaaaaaact
     3181 actttatgat aagttcttat tcgcgagtcg caaatgctat tatgatatag ggttgaaaaa
     3241 atgcaattta tatagggaaa tatatggcaa ttaaaaaaat agcagtatta atcggaagct
     3301 tgcgcaaagg ttcctataat cgcaaagtag cccaagcggt cattaaaatg gcaccttcct
     3361 cgttaaaatt agaaatcgtt gaaataggaa acttaccgct ttataacgaa gatgatgata
     3421 acaatcctcc tgaatcttat gttgccttcc gcgaattaat aaaaaatttt gatggtttac
     3481 tttttgttac ccccgagtac aatcggtcca tacctggagc tcttaaaaat gcattagatg
     3541 taggatccag accttatggt caaagtgcat ggcagcaaaa acccgcagcg attattagtg
     3601 tttctattgg agcaataggt ggatttgcag caaatcatca tttaagacaa gccttggttt
     3661 ttcttgatgt gcctacaatg caacaacctg aagcctatat tggaaacgct gctaatttat
     3721 ttgaagataa tggggagttt aagaataaaa aaactaacga atttcttgaa aaatttatga
     3781 taaattttgc aacttgggtc gaaacaaact gtaaatgact ttactcgtaa tattttgtca
     3841 ggaatgattt gtaataaaag atcaaggatt taatttttaa tccttgatct tgaagcttta
     3901 aggtctaggt gctttaacag gattaaatgc actttgtcca atgaagtgtt ggtacaattt
     3961 ggatacccaa gatgggtttg attcctgttg cgctgcaggc gttattatgg ttgcgtcacg
     4021 tttttcttgt aattttttag caaaagtcac tgcaggttta ttattagttt gtactggctt
     4081 aggcatttta gctagcggcg ctgtgtatcc ggctcctatc ggacaatcac gtttttccat
     4141 gatatttttg gtccaaatat tggcttcatc aaacatcttt tttagaaaag gtgcatactt
     4201 ttcacgtaac agcttttcat cttctggatt tatgattttt ccaggtgcca tctctaatag
     4261 atgttcgtaa ttaatttgct ctttattaag ttctctttct atttttgcaa tatcttgact
     4321 atttgctttt atagattgca gataatttaa tttaagtttt aaaccttcaa ctttttcttc
     4381 aagatctttt ctatcttttt caaaagcttc cttgtcaata tagacaccga tcgcggattg
     4441 gtgctttaaa gtcatttgtt tttttaatcg catctttgtg gcaaaattca tataatctat
     4501 taattcagct ccaaagtctt ctgtgacacg cttggttttg attaaatgtt taacaatttc
     4561 cgttgtattt tgcacgggag cattatagta aaaacctaag ttggttataa attgttcgcc
     4621 gaatcgatat agttcacgtt ttaagtcaat tttttttcca agcttgccat tgataaataa
     4681 ttcacttttc ttttcgtatt tctgtgaaat atcattaatc ataacttcat aagcaatggt
     4741 ttgtctttcg ctaagacccg tttttggatt ctttttgatc aagtgtgatt ctttgcgtgt
     4801 cttaaattta ttatataaac cttcatcgcc aaaaagaaat gcagtatttc ggccaagctt
     4861 gcgtcccgca tctcttgcaa tattttgttc gtgtggagaa taaggtttaa acatttcatt
     4921 agcatagaat tgaatttcgt cacgcaaggc cttaaatgcc ttgctgttgt ttttgataga
     4981 aggatttttt ttaagggctt ggtttagcgc taattgttcc aattcttttt ttcgtttttt
     5041 tatttcattt tttgataaat tattatgagg aaatttttct aaggtaaaat cagcgaactg
     5101 ttctggagtc gccaaaaaag ggcgtgagcc ttcaaaagga attttatccc aatcacgatt
     5161 tttaatcgct tccgttaaaa gttgtttgtt ttgagcaaaa ttggcattac gtgcaaaaaa
     5221 tttaaaatgc ggtggagcat tatctgcttc atccatacgc aaacctttac cgccgatcga
     5281 tgggtgttct cctaaaagat acaatctatc agacacgttt tgcgcgaggg tataaaaata
     5341 tttacgcgct tcaagagaat ctttttcgat taaaaatcca atctccaaat ccgtgatagg
     5401 acccgtttct tggcgagcaa gtgagcctag cgttaaaacc gaaaattgcg taggaggttt
     5461 tcctaatgct tttaactctt gcgtaaatac acggttaatc caagctttgg tatcttgggt
     5521 gatctctcga tttatttcgg tataatcaaa ggttggattc tttaaagctt ccccaatttt
     5581 tttatacgct ttacttcgca ttgcctctaa agtagatttg tccttggcgg atcgcgattc
     5641 aagtgtctgt aattttaaga atggcgattg aatagtttct aaaatgcctt tatacgcttt
     5701 atctacaagc tgttttaaat aactaagcat atcagtatta cctcaaaaaa gtcatttctg
     5761 cagtataaag tgactattgg cagaaaaaac cttaaatcag attaaagctt atttaaaacc
     5821 gctaaaatga gacacgataa cttaaagtta tcgtgtagat gcataggatg gcaatttttt
     5881 attttcaaga tgttctacaa atacatcaat catgggcgcc tgctcaatca aaaaactctc
     5941 gtcaagagac tcaagaaatg cattcttttc aaaatgctct tcaaaagcat ggtaatgggc
     6001 taacgcttct acaaattttg ctatggcatt tgttggataa tccaatcttt tctcagcgca
     6061 gaatttcgct ataattggcg aataatgatt gaatatatca cgctttatca gcgtgtttgc
     6121 taagtgcctt tttaaagaca acgcaaatag cggtaaaagg gcaggtgcca ttagttgttt
     6181 ctctaaatct t
//
LOCUS       DASTIB010000009         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_983373, whole genome shotgun sequence.
ACCESSION   DASTIB010000009 DASTIB010000000
VERSION     DASTIB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983373"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VFP93_00130"
     CDS             <1..1199
                     /locus_tag="VFP93_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9843045.1"
                     /translation="LGYGAFGKVKRIFDKKGNTYVAKVEGGRLEGNNELAILNFLGRG
                     YGSAVRILSAEKEFLEHTIFRKIYTVSELKEGTELFNTLSTADDHGMRIKNLTQIQYL
                     VIALKCCLCIAELHEQRIIHGDLKSENLILQTLPYDLTVHATDFAFSKILPFGQEIIK
                     STPQGTPDFTAPEIIDGGYFSKASDIYALGRMFEKDFNLPPQIWTPMVRSDATQRTTL
                     DESMRMLADYLKFNMPKEDMLAQNALISLREFYDKSTKLQPILQLRPQVVYAFYRNGS
                     NPSLLSWIYPNNRFKKRIFLNGKNQNKEYFLDLLESFCDSIQVDEELNRCDKACLFSG
                     MFQKILYDDNNKSKTIKKMCVDYIRKIDNIYPQASQRVQQDLFKDKLDEIIARISRAT
                     RHRQTF"
     gene            1248..1622
                     /locus_tag="VFP93_00135"
     CDS             1248..1622
                     /locus_tag="VFP93_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843046.1"
                     /translation="MTEKSNVSRDNLWKLHNLFIKKLSEVLRLIAITCIIMCWLFNPL
                     TASMPNAFFCVLSLVLVFFMVDIGHYLVGALTQFIVFKGESSSEKGSLVDIPVYIFVL
                     AKAVILLWAVGVLLNYFMQQYT"
     gene            complement(1619..>2389)
                     /locus_tag="VFP93_00140"
     CDS             complement(1619..>2389)
                     /locus_tag="VFP93_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9843047.1"
                     /translation="NFSTKNLQPAQHFVPNQKLLKNKIILLVGATGGIGQSVAKALAK
                     EQAELILIGRQKKTLESLYDEIVSLGLPEPALYPCDLMNLGIEEASVIREHIAKLYGR
                     LDGYIHCAGAWGTFTPIEYYPEKQWLQVMHLNLNVPFLLIKNMLPLLKLSPLSSIILT
                     ESPLSANTLPLHGAFNCSQYGLRALIAIVKQELQHVSVTITGINPECVSTPLRAKLYP
                     AANDKGILPEQIAPYYVKLLQNPQLYHGKSLTIAELND"
BASE COUNT          744 a          405 c          485 g          755 t
ORIGIN      
        1 atttaggtta tggagctttc ggcaaggtaa agcgtatttt cgataagaaa ggcaatactt
       61 atgtcgcaaa agttgaaggc gggcgcctag aagggaataa tgaactcgct attttaaatt
      121 ttttaggtcg aggctatggt agtgcggttc ggattttatc ggcagaaaaa gaatttctcg
      181 aacacaccat tttccgaaag atctacacgg taagtgaatt aaaggaaggt acggaattat
      241 tcaatacatt gtcaacggct gatgatcatg gaatgcgtat aaaaaatcta acgcagatcc
      301 aatacttagt aatagcatta aaatgctgtc tgtgtatagc agaattacat gaacaacgta
      361 tcatccatgg cgatttaaaa tcggaaaatc ttatattgca aacacttcct tatgatttaa
      421 cagtgcatgc aacggacttc gctttctcca aaattttacc cttcggtcaa gaaattataa
      481 aaagtacgcc acaaggtacc ccagacttta ccgctcccga gatcatagac ggtgggtatt
      541 tttcaaaagc atccgatatc tatgccttag gcagaatgtt tgaaaaagat ttcaatctgc
      601 ctccccaaat atggactcct atggtacgtt ctgatgcaac acaaagaaca acactggatg
      661 agagcatgcg gatgttggcg gattacttaa agttcaacat gccgaaggaa gatatgttag
      721 cgcaaaacgc tttaataagc ttacgcgaat tttacgataa aagcacaaaa ttacaaccca
      781 tattgcaatt gcgaccccaa gtagtttacg ctttttaccg aaacggatct aatccgagtt
      841 tattaagctg gatatatcca aacaataggt ttaaaaaacg cattttttta aacggtaaaa
      901 accaaaataa agaatatttt ttggatttgt tggaaagttt ttgtgattct attcaagtcg
      961 atgaagagct aaatagatgt gataaagctt gtcttttttc gggaatgttt caaaaaattt
     1021 tgtacgatga taacaataaa agtaaaacaa tcaaaaagat gtgtgtggac tatatacgta
     1081 aaatcgacaa catttatccg caggccagcc agcgcgtcca gcaggatttg tttaaagaca
     1141 aattagatga aattatcgcc cgaatcagta gagcaacaag gcatcgccag acgttttaag
     1201 tggtaattta attctttggg aagaggtact ttaaaaaggg aaattatatg actgaaaaat
     1261 ccaatgttag ccgcgacaat ctttggaagc tgcacaattt atttatcaaa aaactttccg
     1321 aagttttacg attgattgct attacgtgca ttattatgtg ttggctgttt aatcccttaa
     1381 cggcttcaat gccgaatgca tttttttgtg tgctatcgct ggtgttagtt ttttttatgg
     1441 tagatatcgg tcattatcta gtaggtgctt taacccagtt tattgttttt aaaggtgagt
     1501 catcatcaga aaagggtagc ttagtcgata ttcctgttta tatttttgtt ctcgccaaag
     1561 cggttatttt gttatgggcg gttggggtat tgctcaatta cttcatgcaa caatatactt
     1621 agtcgtttaa ttcggcaatg gttaacgatt ttccatgata gagttgcgga ttttgtaaaa
     1681 gttttacgta atagggcgca atttgttctg gcaaaatgcc tttatcgttc gctgcagggt
     1741 aaagttttgc acgtagtggg gttgatacac attcgggatt gatcccggta atagtcaccg
     1801 atacatgttg aagttcttgc tttacaattg ctattaaagc acgcagtcca tattgagaac
     1861 aattaaaagc accgtgcaaa ggcagagtgt tggcactcag cggcgattct gtcagaatga
     1921 tggaacttaa gggggataat tttaataaag gtagcatatt tttaattaat aaaaagggaa
     1981 cattcaggtt taagtgcatc acttgcaacc attgtttttc gggataatat tcaatggggg
     2041 taaaagttcc ccaggcgcct gcacaatgta tatagccatc taatcgacca taaagtttgg
     2101 caatatgttc tcgaatcaca gaggcttctt ctatcccaag attcattaaa tcacatgggt
     2161 ataaagcagg ctctggtaga cctaaactta ctatttcgtc atataagctt tctaatgttt
     2221 ttttttgtct tccgatcagg attaattcag cctgttcttt tgctaatgct tttgctacag
     2281 attggcctat accacctgtg gccccgacta acaaaataat tttattttta agaagtttct
     2341 gattaggcac aaagtgttgc gctggttgga gattttttgt agaaaaatt
//
LOCUS       DASTIB010000010         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_685181, whole genome shotgun sequence.
ACCESSION   DASTIB010000010 DASTIB010000000
VERSION     DASTIB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685181"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFP93_00145"
     CDS             complement(<1..277)
                     /locus_tag="VFP93_00145"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HET9843048.1"
                     /translation="MQSAPDTRNLAIQQVGIKSLKHPVVIEDINFQGAPYQFHSVATF
                     SMFVNLPAERKGTHMSRFLTLLNEEADVLLSLKRLPGIIDTLLVRLEA"
     gene            complement(324..2213)
                     /gene="dxs"
                     /locus_tag="VFP93_00150"
     CDS             complement(324..2213)
                     /gene="dxs"
                     /locus_tag="VFP93_00150"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET9843049.1"
                     /translation="MSLLENINSPADLKKISEKDLLLVAKELRTFLIETLDTCGGHFG
                     ANLGTIELTIALHYVFNAPDDHLVWDVGHQAYPHKILTQRKHLLHTIRQKGGLAPFPS
                     RDESIYDTFGVGHSSTSIAAALGISLAKRLANNNSHSIAIIGDGAMTAGMAFEALNHA
                     GSEKADLLVILNDNDMSISENVGALSNYFARILSSKLYTTVKEGSKKVLGRIPPVWEL
                     ARRTESHLKGMVAPGTLFEELGFNYLGPIDGHDLPTLIKMLRNVKEIKGPQLLHVVTK
                     KGKGYLPAEADPIEYHAVNKGFYSSPAQPAPKNNGAPSYSKVFGDWLIDMAQQDPKLV
                     AITPAMREGSGLVEYSSKFPDRYFDVGIAEQHSVTLAAGLACENYKPVVAIYSTFLQR
                     AYDQLIHDVALQNLPVLFAIDRAGIVGDGPTHGGFFDLSYLRCIPNMLIMTPSDANEC
                     RKMLSTGFSYSGPSAVRYPRGSGPNVTMETTLEKLPIGQAKVRRQGQTLALLNFGALL
                     PTALEVGEALDSTVVDMRFVKPLDEELLKDLVKNHRIFFTLEENVIAGGAGSAVSEFL
                     ARNRLPIEVHHLGLPDFFIEHGDRNAILKNIQLDSASILVKIKSILSNYSCQNHDSPV
                     ESQAN"
     gene            complement(2213..3121)
                     /locus_tag="VFP93_00155"
     CDS             complement(2213..3121)
                     /locus_tag="VFP93_00155"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET9843050.1"
                     /translation="MNYNKKPENLLDWMELANQRVLQHLSPYLPNTQEQPSAFNQSLH
                     YVMHNGGKRLRPTLVYMTAAVLGGQLEHCDPAACAVELIHTYSLVHDDLPAMDNDDFR
                     RGKPSCHCAFTEAVAILAGDALQTLAFEILSEPSCSLDDAQRLKMIHTLAKASGLQGM
                     CKGQALDMQYTKTSIPFEALKEMHACKTGALIKASILLGGLSLKNIDQNTYNILDQFA
                     DHLGFAFQVQDDILDIVGNTAILGKPQGSDEAQGKMTIPAILGLEESKKLAHELCESS
                     KKIIRTLNFNSQPLCNLADYIVNRVA"
     gene            complement(3111..3356)
                     /locus_tag="VFP93_00160"
     CDS             complement(3111..3356)
                     /locus_tag="VFP93_00160"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET9843051.1"
                     /translation="MATTKRTKLPNFEKALSSLEAIVESLEKGNLTLEEALKNYEEGV
                     KLTRECQAALKNAQQRVKVLLEENQVLAELKDTADEL"
     gene            3626..4363
                     /locus_tag="VFP93_00165"
     CDS             3626..4363
                     /locus_tag="VFP93_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HET9843052.1"
                     /translation="MAFISIETKLNKSFFTNATAFFLVLISFFVPQPMKGAIADIGFF
                     ALSGAITNWIAIHMLFERVPGFYGSGVIENRFQELKFAIKRMIQEQFFTKHHLQSILA
                     QESDVYLAQIKKLSKILDYEQLYDSLQDAILGSSLGSMLQMFGGQKTLEVIKPKFTEK
                     MEQAITTHLESDQFKDKLAQAIDQEQMTEILQTHLEKIIDSRLEQMTPKMIKILMKQM
                     MEQHLGWLVVWGGVFGGLLGLLKTITV"
BASE COUNT         1334 a          873 c          856 g         1380 t
ORIGIN      
        1 gggcttcaag ccttactagt aacgtatcaa ttatacctgg taatctcttt aaagagagta
       61 atacgtctgc ttcttcgttt aaaagagtta aaaaacgcga catatgtgtg cctttgcgtt
      121 cggcaggcaa atttacaaac atactaaatg ttgcaacaga atggaattgg taaggtgccc
      181 cctgaaaatt aatatcttct atgacaacgg gatgctttaa agattttatt ccaacctgtt
      241 gaatcgctaa atttcttgtg tcgggggctg attgcacatc tggtattaat ttgtttgtcg
      301 acattcaaaa aaaaatcatt tacttaattt gcctgagact ctacgggact atcatggttt
      361 tggcaagagt aattactcaa aatactcttg attttcacca aaatactagc gctatcgagt
      421 tgaatgttct ttaaaatagc atttcggtca ccgtgttcga taaaaaagtc gggtaatcca
      481 agatgatgga cttcaatcgg taatcggtta cgcgctaaaa attcacttac agcacttcca
      541 gcgcctcctg cgataacatt ttcttctaac gtaaaaaata tacgatgatt tttaacaaga
      601 tcttttagta attcctcatc taaaggcttt acaaaacgca tatccactac ggtagaatcg
      661 agcgcttctc ctacttctaa ggcggtaggc agtaatgcgc caaagtttaa tagggcaagt
      721 gtttgtcctt ggcgtcgaac cttggcttgg ccgattggca atttttctag agtggtttcc
      781 atagtaacat ttggcccgct gccacgaggg taacgtacag cacttggtcc tgagtaacta
      841 aaacccgttg ataacatttt acggcattca ttggcatcag aaggtgtcat gatgagcata
      901 ttgggtatac aacgcaagta actcaaatca aaaaaaccac cgtgcgtggg tccatcgcct
      961 acaattcctg cgcgatcgat ggcaaataaa acaggtagat tttgtaaagc tacatcgtga
     1021 atgagttgat cataagcacg ttgtaaaaaa gtagaataaa tcgcaaccac tggcttgtaa
     1081 ttttcgcacg ctaatcctgc agctaaggta acactatgtt gttctgcaat gcctacatca
     1141 aaatatctat ctggaaattt acttgaatat tctacaagtc cagaaccttc acgcatagca
     1201 ggtgtgatag caaccaactt tggatcttgt tgggccatat cgattagcca atctccaaac
     1261 acttttgaat acgaaggagc tccattattt tttggggcag gttgcgctgg tgatgaataa
     1321 aaacctttat taacagcatg atactcgata gggtccgctt ctgcgggtaa atatccttta
     1381 ccttttttgg tgacaacgtg taaaagttgt gggcctttta tttcttttac attgcgcaac
     1441 atttttatta aagtgggcaa atcgtggcca tcgatagggc ctaaataatt aaaaccgagc
     1501 tcttcaaata gggttccggg ggcgaccatc ccctttaggt gcgactccgt tctacgcgct
     1561 aattcccata cgggcggaat tctacctaaa acttttttac tgccttcttt cacagtagta
     1621 tagagtttac tagataaaat acgtgcaaaa tagtttgata gggcccctac attttcggaa
     1681 atagacatat cgttatcatt taagatcact aataaatcgg ctttttccga tccggcatgg
     1741 tttaaagctt caaaggccat acctgcagtc atagcaccat cgccgataat ggcaatggaa
     1801 tgggaattat tgtttgctaa acgcttcgct aaagaaatac ctaaagcagc ggcaatggat
     1861 gtactggagt ggccaacgcc aaatgtgtca taaatacttt catcgcgtga ggggaagggg
     1921 gcaagtcctc ctttctggcg aatagtgtgt aaaagatgtt tgcgttgcgt taggatctta
     1981 tggggatagg cctggtgtcc tacatcccac actaagtgat cgtctggtgc attaaaaaca
     2041 taatgtaaag caatggttaa ttcaatcgtt ccaaggtttg ccccaaagtg gccgccacat
     2101 gtgtccagcg tttcaattaa aaacgtgcgc aattcttttg ccactagaag taagtctttt
     2161 tcagaaattt tttttagatc cgccggagaa tttatgtttt ctaataagga cattatgcta
     2221 ctcgattcac gatgtaatca gcgagattac ataaaggttg actattaaag ttcaaggttc
     2281 gaattatttt tttactactt tcacataatt catgcgccaa tttttttgat tcctctaatc
     2341 ctaatattgc agggatagtc atttttcctt gtgcttcatc acttccttga ggttttccta
     2401 agatcgcggt gttacccacg atatctaaaa tgtcatcttg tacctgaaac gcaaatccta
     2461 ggtgatctgc gaattggtct aaaatattat aggtgttctg atcgatattt ttaagactta
     2521 aaccacccag taaaatactt gctttaatta aagcccctgt tttacaggca tgcatctctt
     2581 tcaatgcttc aaaaggaatg cttgttttgg tgtattgcat atctaatgct tgacctttac
     2641 acattccttg caaacccgat gctttcgcta acgtgtggat catttttaat ctttgcgcat
     2701 catcgagcga acagctaggc tctgataata tttcaaaagc caatgtttga agcgcatccc
     2761 ctgctaaaat tgcaaccgct tctgtaaagg cgcaatgaca agacggcttg ccacgtcgaa
     2821 aatcatcgtt atccatagcg ggtaaatcat cgtgcactaa agaataggta tgaattaact
     2881 cgacagcaca agctgcggga tcacagtgct ccagttgccc acctaaaaca gcagcagtca
     2941 tataaaccag tgtagggcga agacgcttgc ccccattgtg cataacataa tgcaaggatt
     3001 gattaaaagc gctgggttgc tcttgggtat tgggtaaata gggacttaaa tgttgtaaaa
     3061 cacgttgatt ggcaagttcc atccaatcaa ggagattttc aggtttttta ttatagttca
     3121 tcagccgtgt cttttaattc tgcaagaact tgattttctt ccaaaagaac tttgacacgt
     3181 tgctgcgcgt ttttaagagc agcttgacat tccctagtaa gttttacgcc ctcttcataa
     3241 ttttttagag cttcttctag ggttaaattt cctttttcca aagactcaac gatcgcttcc
     3301 aaagagctta gcgctttctc aaagttaggg agcttggtgc gttttgtagt agccataaat
     3361 cgctaagtat gaaaacatga aacttaaccc agaagatacc cagcgtcaac gtaggttttt
     3421 agaaattacc aatgttagtc agtggaattt aaaaagttaa ccttttaact gaaaattgtt
     3481 accttcggtc catttaccaa gattgctctt taggtttgtt tagcaattaa ccaatgacca
     3541 aaagccactt atcgagcaaa cctgtaaaac gagcgcggtt tttgttaaac tacacatctc
     3601 tgatcaaagc agcaatcaat gttatatggc attcatttcc atcgaaacaa aacttaataa
     3661 aagttttttt actaatgcca ccgctttctt tttagtactt ataagttttt ttgtaccaca
     3721 accgatgaaa ggggcgatag cagatattgg ctttttcgca ttatctggcg ctatcaccaa
     3781 ttggattgca atccatatgt tgtttgaacg cgttcctggt ttttatggtt caggcgtcat
     3841 agaaaatcgt tttcaagaat taaaattcgc cattaaacgc atgatccaag aacaattttt
     3901 taccaaacat catttacaat ctattttagc gcaagaatct gatgtttatt tagcacaaat
     3961 taaaaaattg agcaaaattt tagattatga acaactctac gattctttac aggatgctat
     4021 tttaggctct tccttaggca gtatgctcca aatgtttggt ggtcaaaaaa cattggaagt
     4081 cataaaacct aagtttacag aaaaaatgga gcaggcaatt accacccact tagaatctga
     4141 tcaattcaaa gataagttgg cacaagcgat cgatcaagaa caaatgacag aaatcctgca
     4201 aacccattta gaaaagatca ttgatagccg tttagaacaa atgacaccta aaatgataaa
     4261 aatattaatg aaacaaatga tggagcaaca tttaggttgg cttgtagttt ggggtggcgt
     4321 gtttggaggt ttgcttgggt tgttaaaaac cattaccgtt taaaagtgaa tactatgaaa
     4381 gcagcaaaat atgacgatta tttaagtttt ttacgcgaat ccttttcgga tctttgcaaa
     4441 ttt
//
LOCUS       DASTIB010000011         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_923472, whole genome shotgun sequence.
ACCESSION   DASTIB010000011 DASTIB010000000
VERSION     DASTIB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923472"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFP93_00170"
     CDS             complement(<1..519)
                     /locus_tag="VFP93_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843053.1"
                     /translation="MFKLKHLFCGLFLILGLSFTLEANLYEFFLNPYVGVEGAWRHWP
                     WDAEFGERHFKENYMNHNGVIGLQFHTFFAIEAGYQTTDRQQKQKFYNGDGFTAEPVL
                     GFLRPFGDRAPVLHIAEARMSGPHINLIGLLPILNATTLYASLGVAWMRFYVSTVGIN
                     DDVANIVSIPVTR"
     gene            1106..>2007
                     /locus_tag="VFP93_00175"
     CDS             1106..>2007
                     /locus_tag="VFP93_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843054.1"
                     /translation="MRYSNFQLNQIHAVIINEQKEQWQNALQEIITPTVVSRFDDNTN
                     RSTPPNNIPPPPPTIKNKNITVQCPEEIIQLQDILARIHNSNVTQNIVNSLEDELKTR
                     SVEFCLAHKNMQGIRDTATKQFWLNGAIALAFNTKIKNCVRGSEQDNETKNYRKEAET
                     VIENVFINLSNDKTIDQLTNFFLACFRQRNSENGFESIYELAQKTWKKISTNKVEAEI
                     TNDLEMQDQLVKINTQKEKFLEMHENLKEAILGKQLKAENYKEDPRYKALQIIIKERD
                     ALANSYKVLINLEKDLEKKIQRLE"
BASE COUNT          769 a          347 c          308 g          583 t
ORIGIN      
        1 acgagtaacg ggtatagata ctatattagc gacgtcatcg tttattccta cggtactaac
       61 gtaaaatcgc atccaagcaa ctcctaatga agcatataaa gttgtagcat ttaatatagg
      121 taaaagtcca attaaattta tatgaggccc actcatacgt gcttctgcaa tatgtaaaac
      181 aggggctctg tctccgaatg ggcgcaagaa acctaataca ggctctgcag taaaaccatc
      241 accattataa aatttctgct tctgttgtcg gtccgtggtt tgataacccg cttcgattgc
      301 aaaaaaagta tgaaattgaa gcccaattac tccattgtga ttcatataat tttctttaaa
      361 atgacgctcg ccaaattctg catcccatgg ccaatgacgc caagcacctt ctactccaac
      421 atagggattt aaaaagaatt catataaatt cgcttcaagc gtaaaagaga ggcctaaaat
      481 tagaaacaat ccacagaata aatgtttcaa tttaaacatg ttaacttcct tgtgattttc
      541 taggaagcat aacaatgtgc tctaagatat tcaatgtaaa aaagaagatt gcaaactgac
      601 aaatattttc gaattcatta aattaagtaa ttcattaagg aaataggata ttatcgggca
      661 aaaatttatt tttactattt tagcaagctc acaacaattt cttttataac actttaccaa
      721 aaaatccttg caagatttta acaactgaac caagttttta atccttatag ctttttactc
      781 gctttttaaa taatcatact gatttattat tgtagccact gtcgtcccgc acggaggctg
      841 tctgccttta atgataagaa tgtataaatt tcacagccat agatgcagca tacagattaa
      901 aatttttttt acagaataat ttttagggaa agacctgatt tctaacataa taagatcatt
      961 caattacatt cttttttcgc caggtttttt attactgtaa cagaccaaca gcacaattaa
     1021 accgatacag gttggtttta taacttcttt tgtgtttcaa aattgctaca atagctctcc
     1081 aatgtaagcc ttatagttgt aaaaaatgag atacagtaat tttcaattaa atcaaataca
     1141 tgcagtgata ataaacgaac aaaaggaaca atggcaaaat gcgttgcaag aaattattac
     1201 acctactgta gtctcacgct ttgatgataa tactaatcga tccactccac ccaataacat
     1261 tccgccaccg ccacccacaa taaaaaacaa aaacatcacg gttcagtgtc cggaagaaat
     1321 tatacaatta caagatattt tagcgagaat acataattct aatgttacac aaaatattgt
     1381 aaatagttta gaagacgaat taaaaacgcg ctccgtagaa ttctgcttgg ctcataaaaa
     1441 tatgcaaggt atacgggata cagctacaaa acagttttgg ctaaacggtg ccatagcttt
     1501 agctttcaat acaaaaatta aaaattgcgt tagaggttca gagcaggata acgaaaccaa
     1561 aaattatcgc aaagaagctg aaactgttat tgaaaatgta ttcattaatt tgtcgaatga
     1621 caaaaccata gatcaactaa ctaatttttt cctcgcatgt tttcggcaaa gaaatagcga
     1681 aaatggtttt gaatcgattt atgaattagc acaaaaaact tggaaaaaaa tcagcacaaa
     1741 caaggttgaa gcagaaataa cgaatgatct agagatgcaa gatcaacttg taaaaattaa
     1801 tacacaaaaa gaaaaatttt tggaaatgca cgaaaattta aaagaagcca tcttaggtaa
     1861 acaattaaaa gcagaaaatt acaaagagga tccgcgttat aaggcattgc aaattattat
     1921 taaagaacgg gatgctttag caaatagcta taaagtatta ataaacttag aaaaagactt
     1981 agagaagaaa attcaacgac ttgaaaa
//
LOCUS       DASTIB010000012         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_461029, whole genome shotgun sequence.
ACCESSION   DASTIB010000012 DASTIB010000000
VERSION     DASTIB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461029"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VFP93_00180"
     CDS             <1..174
                     /locus_tag="VFP93_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9843055.1"
                     /translation="TSFNVRGEPIVCTPKDAYRCFMRTEMDYLIMENIILKKNNQPQY
                     QDTTDWIKEFLLN"
     gene            192..572
                     /locus_tag="VFP93_00185"
     CDS             192..572
                     /locus_tag="VFP93_00185"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HET9843056.1"
                     /translation="MTNLRKFGLLLGSIFVALFGMLIPVIRSNPYPEWPWFLAMGFWV
                     PALIYPKALSLVYTYWMKMGEFLGYINTQLLLGLLFFTIITPIGIMKRFFGNDIIGKQ
                     FDKNLTSYAKRIESRPVKHMEDPF"
     gene            572..724
                     /locus_tag="VFP93_00190"
     CDS             572..724
                     /locus_tag="VFP93_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HET9843057.1"
                     /translation="MFELLNDLWRFMCIRKKFWLAPILVIMLMLGTLIVLTQGSAASP
                     FIYTLF"
     gene            complement(783..2192)
                     /locus_tag="VFP93_00195"
     CDS             complement(783..2192)
                     /locus_tag="VFP93_00195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET9843058.1"
                     /translation="MRVTVFGSGYVGLVVAACLAETGNQVICVDVNKVKIQKLQHGII
                     PIYEPGLQEVVQRALVAKKIEFTTDANKAISKAEFIFIAVGTPQDQDGSADLQYVLNV
                     AQKIGEYLQDYTVVVNKSTVPVGTADKVQAKIQSVLQARNINIPFDIVSNPEFLKEGE
                     AIEDFTKPDRIVLGLRSARAEKKMRTLYAPFAHNPERLIIMNNRSAELTKYAANVMLA
                     TKISLMNQLANLAEKVDADIDQVRLGIGSDPRIGPHFIYSGCGYGGSCFGKDVKALIK
                     TAAEVQSNASILQAVESVNLDQKRIVFDKIVAHFNFKLADKTLAIWGLAFKSGTDDMR
                     DAPSRVVLELLWQQGAKIQAFDPKAMEEAQRIYGKRADFTLAHTPEEALEGADALVFL
                     TDWMVFRSPSFETMRKLLKNPVIVDGRNLYDPIQMEQMGFIYYGIGRGKNTASKTYVD
                     LLGSIRTDFNIKKSVADEV"
     gene            2283..>2686
                     /locus_tag="VFP93_00200"
     CDS             2283..>2686
                     /locus_tag="VFP93_00200"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013460983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9843059.1"
                     /translation="MRKRILVTGGAGFLGSHLCKKLLESGNEVICLDNFYTGNKDNLR
                     TVMRHPYFELIRHDITEPIQLEVDAIYNLACPASPVHYQYDPIKTTKISVLGALNVLE
                     LAHRLKCPVLQASTSEVYGDPQVHPQEESYWG"
BASE COUNT          800 a          536 c          477 g          873 t
ORIGIN      
        1 acttctttca acgtgcgagg tgaacctatt gtgtgcacac caaaagacgc atatcgttgt
       61 tttatgcgaa cggaaatgga ttatcttata atggaaaata ttatcctaaa aaaaaacaac
      121 caacctcaat atcaagatac aaccgattgg attaaagagt ttcttttaaa ttgatgatcc
      181 aggcagaaaa tatgacaaat ctacgtaagt ttggcctttt gctgggcagt atctttgtgg
      241 cgctttttgg tatgctgatt ccagttattc gctccaatcc ttatccagag tggccatggt
      301 ttttagccat gggattttgg gtccctgctc tcatttatcc taaagcactt agcttagttt
      361 atacatattg gatgaagatg ggggaatttt taggctatat aaatactcaa ttactgttgg
      421 ggttgctgtt tttcacaatt attactccca ttgggataat gaagcgattc tttggcaacg
      481 atattatagg aaaacagttt gataaaaatt taacgagtta tgcaaaacgc atcgagtcaa
      541 ggcctgtaaa acatatggag gatcctttct aatgttcgaa ctcttaaacg atttatggcg
      601 atttatgtgt attcgaaaaa aattttggtt agcaccaatt ttggtgataa tgctaatgtt
      661 aggaacatta atcgtgttaa cacaaggatc ggcggcttct ccttttattt atacattgtt
      721 ttaataaaat taaataaata agctttaaac caagaaatca atatttaacc aggcttctct
      781 acttatactt catcagccac tgatttcttt atattaaaat cggttctaat cgaacctaat
      841 agatccacat atgtttttga agcggtattt tttcctcgac caattccata ataaataaac
      901 cccatttgct ccatttgaat aggatcatac aaattgcgac catctacaat tacaggattt
      961 tttagcaatt tacgcatggt ttcaaaacta ggactgcgga aaaccatcca atctgtaaga
     1021 aacactaaag catccgctcc ttcaagagct tcttcaggag tgtgcgctag ggtaaaatcg
     1081 gctctttttc cgtagattct ttgggcttct tccattgctt taggatcgaa tgcttgaatt
     1141 tttgcgccct gttgccataa taattctaaa actactctac ttggcgcatc gcgcatatca
     1201 tccgtaccgg atttaaaagc caacccccag attgcaaggg ttttatctgc taatttgaag
     1261 ttgaaatgtg ccactatttt gtcgaatact atccgttttt gatctaggtt taccgattct
     1321 acagcctgta aaatcgatgc attgctctga acctccgctg cggttttgat caaagctttt
     1381 acatctttcc caaagcacga cccaccatag ccacaacctg aataaataaa gtgcggtccg
     1441 attcggggat cgctgccaat accaagtcgc acttgatcga tatctgcatc tactttttca
     1501 gccaaattgg ctaattgatt cattaaacta attttagttg ctaacataac atttgcggca
     1561 tatttcgtaa gttcggcgga gcgattattc atgataatta aacgttctgg attatgtgca
     1621 aaaggcgcat acagagttcg cattttcttt tccgcacgcg cagatcttaa acccaatacg
     1681 attcgatcgg gtttggtaaa gtcttctatt gcttcacctt cttttaaaaa ctcaggattt
     1741 gaaacaatgt caaaaggaat atttatatta cgtgcttgca acaccgattg aattttagct
     1801 tgaactttat cggcagtacc cactggtacc gtcgacttgt taactacaac cgtataatct
     1861 tgaagatatt caccgatttt ttgagcaaca ttgagtacat attgtaaatc cgctgagcca
     1921 tcttgatctt gtggggtacc gactgcgata aaaataaatt cggctttgct aattgcttta
     1981 tttgcatcag ttgtgaattc aatttttttt gcaaccaaag cacgttgcac tacttcttgt
     2041 aatccgggct cataaatagg aatgatccca tgctgaagtt tttgtatttt tactttgtta
     2101 acatctacgc atatgacttg gttgcctgtc tcagctaaac aagccgcaac cactaaaccc
     2161 acatatcctg atccaaatac tgttacgcgc attcgttatc ctaaagttac aattgagcac
     2221 tatcatagat ctaacttttg tagtctgcta gactcagatc aaattataaa ggctggcgca
     2281 gcatgagaaa aagaatttta gtaacaggcg gtgcaggatt tttaggatcc catttatgca
     2341 aaaaactact tgaatctggc aatgaggtta tttgcttaga taatttttat acgggcaata
     2401 aagataatct ccgtactgtt atgcgtcatc cctattttga gcttattcga cacgatatta
     2461 ctgaacctat tcaacttgaa gtagatgcga tttataattt agcttgtcca gcaagcccgg
     2521 tacattacca atatgatcct ataaaaacta caaaaataag cgtgttaggt gccttaaacg
     2581 ttttagaatt agcacaccgc ttaaaatgcc cagtattgca agcatcaact agtgaagttt
     2641 atggagaccc tcaagtccac ccccaggaag aatcttattg gggcca
//
LOCUS       DASTIB010000013         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1221256, whole genome shotgun sequence.
ACCESSION   DASTIB010000013 DASTIB010000000
VERSION     DASTIB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221256"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /locus_tag="VFP93_00205"
     CDS             <1..2097
                     /locus_tag="VFP93_00205"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET9843060.1"
                     /translation="TSLYREITAIWLTDEIRRKRPTPREEAKWGFAVIENSLWHAIPQ
                     YLRELDHVCKIYTGQALPLNAIPIRFGSWMGGDRDGNPNVTHQVTQQVCFLGRWSAAN
                     LYEKAMRNLIAELSMKDCDAEVRKIVGDHPEPYRALLKPVREILKEDLQTLENCLADQ
                     GMEGVLKIIAIDQLLEPLLLCYNSLKKIGAAIIAEGSLLDLIRRINCFGLTLARLDIR
                     QEASRHQGLMQEILTQNRLHRRFNEEDSVEWLLNVCINPAQLTIDPKLFTTDSLEVWQ
                     TILLINQQPPDTFGAYVISMTKKPVDIIIVYALQKLAGVVEPIRVVPLFETLADLQSA
                     SHCLETLFRTDSYRNLIRSKQEVMIGYSDSAKDAGIIAANWAIYLAQEQCLKIAEKYD
                     IQLTLFHGRGGTVGRGGSPAYMAIVSQPPGAVHGSMRITEQGEVIRTKFGFTKNAIRT
                     LELYTSAIFEAYCNKPPVPQKNWRVLMDKIAQASCSFYRNTIYKDPKFVPYFFAVTPI
                     DEIGKLFISSRPVKRGSKAGIEVLRAIPWIFAWTQNRMMTPSWLGVGDALQSLNGQEK
                     IVLRDMVEKWPFFKSLISLIEMVIAKTDPSIALHYEQCLAPFQLKEIGEKIRNDFNHT
                     YQIVKELLKIHNLLESNNTLDRSLKVRQPYVYPLNILQAELLAKVRNGEASSSEYHEA
                     LLISISGIAAGMRNTG"
BASE COUNT          749 a          405 c          475 g          671 t
ORIGIN      
        1 acttcccttt atcgcgaaat tactgctatt tggttaaccg atgaaataag acgaaaacgt
       61 ccaacaccta gggaggaggc gaagtggggt tttgcagtta ttgaaaatag tctttggcat
      121 gctatcccac aatatcttcg agaacttgat catgtatgta aaatttatac gggacaagcc
      181 ttacctctta atgcaatccc cattcgtttt gggtcctgga tgggagggga tcgcgatgga
      241 aatcctaatg tcacccacca agtgacccaa caagtatgtt ttttgggaag atggtcagcg
      301 gctaatttgt atgaaaaggc tatgcgaaat ctcattgcgg aactttctat gaaagattgt
      361 gatgcagagg ttcgaaaaat agtgggtgat catcctgaac cgtatagggc cttgctaaaa
      421 cccgtgcgag aaattttaaa agaggattta caaacgttag aaaattgctt agcggatcag
      481 ggaatggaag gagttttaaa gataatagcc attgatcaat tactagaacc tttgctgctg
      541 tgctataact ccttaaaaaa aattggagcc gctattatag cagaaggtag cttattagat
      601 ttaataagaa gaattaactg ctttggttta acgctagcgc gtttggatat tcgacaagaa
      661 gcaagccgtc atcaaggttt aatgcaggag atattaacgc aaaatagatt acatcgtaga
      721 tttaatgaag aagattctgt cgaatggtta cttaacgttt gtataaatcc tgcgcaacta
      781 accatcgatc ctaaattatt taccaccgat tctttagaag tttggcaaac tattttgctt
      841 attaatcaac agccacccga tacttttggc gcctatgtaa tatcaatgac caaaaaaccg
      901 gttgatatta tcatagttta tgcactacaa aaattagcgg gagtcgttga acccattaga
      961 gttgtaccat tatttgaaac attagccgat ttacaatcgg cttcccattg tttagaaacg
     1021 ttatttcgta ctgatagtta tcgtaatttg ataagatcta aacaagaggt catgattggt
     1081 tactcggatt ccgcaaaaga tgcaggcatt atcgctgcca attgggcaat ttacttagcg
     1141 caagaacaat gcttaaagat tgccgaaaaa tacgatattc aattgaccct ttttcatggt
     1201 cgaggtggta cggtaggaag aggtggatca cctgcgtaca tggccatagt atctcaacca
     1261 ccaggagcag tgcatgggag tatgcggatc acggaacaag gtgaggtaat tcgtacaaaa
     1321 tttggattta ctaaaaatgc tattcgaacc ttagagctat ataccagcgc gatattcgaa
     1381 gcatactgta acaaaccacc ggtcccgcaa aaaaattggc gggttcttat ggataaaatt
     1441 gcgcaggcct catgctcttt ttatcgaaat acgatttata aagatccaaa atttgtacca
     1501 tacttttttg cggtaacccc tattgatgaa atcggcaaat tatttattag ctctcgacct
     1561 gtgaaaaggg gaagtaaagc cgggattgaa gttttgcgcg cgataccgtg gatttttgca
     1621 tggacgcaaa atcgcatgat gactccatcg tggttaggtg tgggggatgc attacaatcg
     1681 ttaaatggac aggaaaaaat tgtattacgc gatatggtag aaaaatggcc tttttttaaa
     1741 tcgcttattt ctcttataga aatggtaata gcaaaaaccg atccatctat tgcattgcat
     1801 tatgaacaat gtttagcgcc attccagttg aaagaaatag gtgagaaaat tcgcaacgat
     1861 tttaatcata cgtatcaaat agtaaaggaa ttattaaaga tacataattt attagaaagt
     1921 aataacaccc tcgatcgttc tttaaaggta cgccagccct atgtttatcc tttaaatatt
     1981 ttacaagcag aactattagc caaagtgcgt aacggtgaag cttccagctc agagtatcac
     2041 gaagctttac taattagtat tagcggcatt gcagcaggca tgcgaaatac tggctaactt
     2101 cgttgtcatc cggaaatagg gttgctatgc ttaaaaagca ccaaaatttt gtcggataac
     2161 acatgttaac tgtggcagcc agattaaaaa aacttttcga tttttatcga attcattatc
     2221 aatttataag tcatgaaagg acgattacgt taatagaagc acaagaatgc acacaaattg
     2281 ataaaaaata ttttgtcaga
//
LOCUS       DASTIB010000014         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_297203, whole genome shotgun sequence.
ACCESSION   DASTIB010000014 DASTIB010000000
VERSION     DASTIB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297203"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFP93_00210"
     CDS             <1..268
                     /locus_tag="VFP93_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843061.1"
                     /translation="AHTLPEPVKSRTVAIRVQHATFKFTATPKEQRILDEAGAEGVRS
                     LINQMLLNQRNSYQNVLQHEQHQHQLKQNQNKTPRKPVPLPKKR"
     gene            359..1075
                     /gene="ung"
                     /locus_tag="VFP93_00215"
     CDS             359..1075
                     /gene="ung"
                     /locus_tag="VFP93_00215"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9843062.1"
                     /translation="MAATKSKNPYKDPELESSWKAVLGEEFGKPYMVKLREFLLQEKA
                     AKKKVFPHSTDIFKAFELTPFDKVKVVILGQDPYHGINQAHGLCFSVKPGVAMPPSLI
                     NIYKELKEDLNITPPKHGCLACWAKQGVLLLNCVLTVEQGLAASHQGKGWEDFTNKVI
                     ELLNRSNRPLAFVLWGSYAQRKGEAIDENRHLVIRSAHPSPLSAHRGFFGSKPFSKIN
                     QFLEKTDQTPIDWQITEESE"
     gene            1130..2095
                     /gene="glsA"
                     /locus_tag="VFP93_00220"
     CDS             1130..2095
                     /gene="glsA"
                     /locus_tag="VFP93_00220"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HET9843063.1"
                     /translation="MDRDKYIEHIKNILDRAVLESRALTSGTVANYIPELANVDPELT
                     SSAIILSDGSAIAAGDVSEHLFTLQSVAKLIVLIGLMEEYGEEKIFSWIHAEPTGQHF
                     SSLAQVDRYGPIPANPMVNAGAIALCSRIPGDTLTRALWLDQWVERLFGKHLNVNGKV
                     FASERTSGDRNRSIAYLLKSNNDLALPVDEALETYFYLCSYEATILTAAYLPMILANG
                     GLAPDGNRIISERTSNAVVAIMATCGLYDESGMHLVKTGMPSKSGVSGLVVAVATGRG
                     GIAVSSPRLNVKGGSVRGHAILHQVSYELDWHFAAPWGYLRIQDE"
     gene            2185..2673
                     /locus_tag="VFP93_00225"
     CDS             2185..2673
                     /locus_tag="VFP93_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET9843064.1"
                     /translation="MSDKKIESKNEAMQLAIDSAFVGLKNKEGGPFGAAILQNGKVIA
                     VAHNTVLKDKDPTCHSEINAIRQACKNLNTHILSGCEIYSTAEPCPMCLAAIYWARLD
                     KIFIGVDKSIAAKYGFDDAKFYEQILLGLERREIPIQCGILAKECEEVFSHWKEIEGV
                     LY"
     gene            complement(2670..>2941)
                     /locus_tag="VFP93_00230"
     CDS             complement(2670..>2941)
                     /locus_tag="VFP93_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843065.1"
                     /translation="PCHIAKPNKLKKILQLNFDGIELYSANINKDKVSWLEHQIKNTK
                     FFVTGGSDFHKKQSPLHLGISYTSEEIFGMLQQKCAENNKALHGN"
BASE COUNT          916 a          548 c          617 g          860 t
ORIGIN      
        1 tgcgcatact ttgccagagc ctgtgaagtc tcgcactgtt gctatccggg tgcagcatgc
       61 aacatttaaa ttcaccgcta ccccgaaaga gcaaagaata ttagatgaag ctggagcaga
      121 gggcgtacgt tcgctcatta atcaaatgct tttgaatcaa agaaattctt accaaaatgt
      181 tttacagcat gaacaacatc aacatcagtt aaaacaaaat caaaataaaa cacctcgcaa
      241 acctgtaccg ttgccaaaaa aaagataatt ttctaactga attaaaccca ttttaaggtt
      301 gcgaaatgtt ccttcgcgcg ttattctata cagttaatgt tatctaagga acataagttt
      361 ggcagcaaca aaatctaaaa atccatacaa ggatcctgaa ttagaatcct cctggaaagc
      421 cgttttgggg gaagagtttg gaaaacctta tatggtaaaa ttgcgagaat ttttattgca
      481 agaaaaagca gccaaaaaaa aagtatttcc tcacagcacc gatattttta aagcttttga
      541 attaacacca ttcgataagg tcaaggtcgt tattttaggg caagatccct atcatggcat
      601 aaatcaggcc catggtttgt gtttttccgt aaagcctggg gtagcgatgc caccatcact
      661 tatcaacata tataaggaat taaaagagga tttaaatatt acgccaccta aacatggttg
      721 tttagcatgc tgggcgaaac aaggtgtatt gttattaaac tgcgttttaa cagtcgagca
      781 aggtttagcg gcttcgcacc aaggcaaggg atgggaagat ttcactaata aagtaataga
      841 attgttgaat cgatccaaca gacccttagc ctttgtatta tggggatcgt atgcccaacg
      901 caagggggag gcaatcgatg aaaatcgaca tttagttatt agatcagcac atccttcacc
      961 cttatcagcg catcgtggat tttttggcag taaacctttt tcaaaaatta accaattttt
     1021 agaaaaaact gaccaaaccc caatcgattg gcaaattaca gaagagtcgg aataagctca
     1081 tctataatta ggtaagtttc ctgtgtatta atcacattat gaagtagtta tggatcgcga
     1141 taaatatatt gaacatatta aaaacatttt ggatcgcgcg gttttggagt cgcgagcatt
     1201 aacttcgggt actgtggcaa actacatacc cgaacttgcc aatgtggatc ctgaattaac
     1261 ctcaagtgcc atcatactgt ccgatggtag tgctattgca gccggtgatg tatctgagca
     1321 cctatttact ttgcagtccg ttgcgaaact catcgtttta attggcttga tggaagaata
     1381 cggtgaagaa aaaatctttt cttggatcca tgcagagccc accggacaac atttttcatc
     1441 gcttgcgcaa gtggatcgct atggacccat tcctgctaat cctatggtaa atgcgggtgc
     1501 tatcgctttg tgtagccgta ttcctgggga tacattaacg cgcgcgttgt ggttagatca
     1561 gtgggttgag cgtctattcg gcaagcattt aaatgtgaat ggaaaagtgt ttgcatcgga
     1621 gcgaacctcg ggggatagaa atcgatcaat cgcctattta ttaaaaagca ataacgattt
     1681 agccttgccc gtagatgaag ccttagaaac gtatttttac ttatgttcct atgaagccac
     1741 tatattaact gctgcctatt taccgatgat tttagccaat ggtggtttgg caccagatgg
     1801 caatcgtatc atatcggagc gcaccagtaa tgcagtagtg gctattatgg caacctgcgg
     1861 tttatacgat gaatccggca tgcatttagt aaaaaccggc atgccctcca aatcaggggt
     1921 atcgggctta gtggtcgcag tagccacggg tcgtggtggt attgccgttt caagcccccg
     1981 cctcaatgta aaaggaggta gtgttagagg ccatgcaata ttacaccaag tttcttacga
     2041 actagattgg catttcgccg caccttgggg ctatttgcgc attcaagacg aatagtgaat
     2101 catgaaaacc aacacttgag acatccataa tatttatgga tgttctcttt tggtatacgc
     2161 aaaaataaca tcttaaggaa acgcatgtca gataagaaaa tcgaatctaa aaacgaagcg
     2221 atgcagcttg ctattgatag tgcatttgtg gggttaaaaa ataaagaggg cggtccgttt
     2281 ggcgcagcta ttttgcaaaa tggcaaagta attgcggtag ctcataatac ggtattaaaa
     2341 gataaagatc ctacttgtca ctcagaaatt aatgcaatcc gacaagcgtg taaaaattta
     2401 aacacacata ttctcagcgg ttgcgaaata tattcaacag cggaaccatg tcctatgtgt
     2461 ttagcggcaa tttattgggc tagacttgat aaaattttta ttggtgtcga caaaagtatt
     2521 gcagcaaaat atggattcga tgatgcaaaa ttttatgaac aaatactgct tggcttagaa
     2581 cggcgtgaaa tacctattca atgcggcatt cttgcaaaag aatgtgaaga agtattctcg
     2641 cattggaaag aaatagaagg cgtattgtac taatttccgt gtaatgcctt attattttct
     2701 gcgcattttt gttgtaacat tccaaaaatt tcctctgagg tataggaaat gcctaaatgc
     2761 aaaggagatt gctttttgtg aaaatcagaa cctcccgtca caaaaaactt tgtatttttg
     2821 atttgatgct ctagccagga aactttatct ttgtttatat ttgcggaata taattctatt
     2881 ccatcaaaat tgagttgcaa gatttttttt aatttattgg gcttggctat gtgacaaggg
     2941 t
//
LOCUS       DASTIB010000015         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1057291, whole genome shotgun sequence.
ACCESSION   DASTIB010000015 DASTIB010000000
VERSION     DASTIB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057291"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFP93_00235"
     CDS             complement(<1..1059)
                     /locus_tag="VFP93_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843066.1"
                     /translation="MHRTITFFWRKVLVYFFVVSLSFGMVVLSPITNATIRTDSSDIQ
                     KKMGQLDKSLLTLEDKIKRSQHDLSKEEKALAKIDTSIHDTLIHIKTIEKEMRESEKE
                     VERLNSDIFTFKNQQQKKIRALELYLLSQQKHQDNHKLKFILNQKSIHDMVVLNKHYN
                     SFSYALTQQIQQLIHNLKNLDHQQQSLERKRTELQELAKELSAKKEDFNKKKTLQKQS
                     ISQYQSSIQKNLDEKKNLFNEKQALNKLLKEIQIALEKQKKDEMERKKQRQQEEQKHK
                     ITTKIASHRIPEHVKDSANIGFGKMLGKLPSPLKNSKLKNKKNNSYYLAQEGSIVQAI
                     YPGKVVFSEWLKGIGLLLI"
     gene            complement(1044..>2463)
                     /gene="gpmI"
                     /locus_tag="VFP93_00240"
     CDS             complement(1044..>2463)
                     /gene="gpmI"
                     /locus_tag="VFP93_00240"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9843067.1"
                     /translation="MLLNASGAEVGLPEGQMGNSEVGHLCMGAGRVIYQDLNRIQHDI
                     KTGNFSLNPVLVELFNTVKTSQRALHILGLLSEGGVHSHSDHFMAVLEFARKHDIKNI
                     YVHAFLDGRDTAPQSAYAVLEKMSTWFENYGFGQIASICGRFYAMDRDKRFDRTQLAY
                     DLLTEGLGYAATNALEGLRMAYERGESDEFVKPTVITNTDQPITIQDGDCVLFMNFRA
                     DRARQLSYALSHKNFTDFERKKFPNLEEFVTLAEYDAKLPAKVLYPPEQFPKVLGEVL
                     QDNGLKQLRLAETEKYAHVTFFFNGGVEEPFNGEDRVLIPSPKVTTYDLKPEMSAYQV
                     TEELTKAILAQSYDVIICNFANADMVGHSGNLSAACEAVKVLDHCLGKIVEALQAVDG
                     SAFITSDHGNVEMMQNPITSLPHTAHTHEAVPLVYFGKQSIQFKRSMGKLADIAPTLL
                     YSMGLSIPSVMTGEVLIECTEQ"
BASE COUNT          693 a          473 c          442 g          855 t
ORIGIN      
        1 gattaaaaga agacctatgc cttttaacca ttctgaaaaa accacttttc caggataaat
       61 cgcctgaacg attgagcctt cttgcgctaa ataataagaa ttattttttt tattttttaa
      121 tttggagttt ttcaaagggg aaggtaattt gcccaacatt ttaccaaacc ctatattcgc
      181 agagtccttt acatgttctg gaatgcgatg ggaagctatt ttggtggtta ttttatgttt
      241 ttgttcttcc tgctgtcgtt gttttttgcg ctccatttca tctttttttt gtttttctag
      301 agcgatttgt atttctttta gtagtttatt aagagcctgc ttttcattaa atagattttt
      361 tttctcatct agattttttt gaatggaaga ttggtattgt gaaatagatt gtttttgcaa
      421 agttttcttt ttattaaaat cttccttctt cgcggataat tctttggcta gctcttgtaa
      481 ttcagtccgt ttgcgttcta aagattgttg ttgatggtct aaatttttta aattatgaat
      541 aagttgctga atctgctggg taagcgcata cgaaaatgaa ttgtaatgtt tattgagtac
      601 aaccatatca tgaatcgatt tttgattaag aatgaatttt aatttatggt tatcttggtg
      661 cttttgttgc gaaagtaaat acaactccaa tgcgcgtatt tttttttgtt gttggttttt
      721 aaaagtaaaa atatcagaat tcaagcgttc aacttctttc tcactttcgc gcatttcttt
      781 ttcgatggtt tttatgtgaa ttaaagtgtc atgaatggaa gtatcaattt ttgctaaagc
      841 tttttcttct ttggacagat cgtgttgcga acgtttaatt ttatcctcta aggtaagcaa
      901 cgatttatct agttgtccca tttttttttg aatatcggac gagtctgtcc taatggtagc
      961 gttggtgata gggctcaaca ctaccatacc aaaagataac gatactacga aaaaataaac
     1021 aagcactttt cgccaaaaaa aggttattgt tctgtgcatt cgattaacac ttctccagtc
     1081 ataacagaag gaatagataa tcccatacta tataacaatg taggtgcgat atccgctaat
     1141 tttcccatcg atcttttaaa ttgtatggat tgttttccaa aatataccaa gggcacggcc
     1201 tcgtgggtat gggcggtatg cggaagactt gtgattggat tttgcatcat ttctacattg
     1261 ccatgatcgg acgtaataaa agcgctgcca tccactgctt gcaaagcttc tacaatctta
     1321 cccaaacaat gatctaatac cttaacagcc tcgcaggcgg cagacaaatt cccactatgt
     1381 cctaccatat cggcattggc aaaattacag ataatgacat cataagattg cgccaagata
     1441 gctttagtga gttcctctgt aacttgatac gcgctcatct cgggttttaa atcatacgtg
     1501 gttacctttg gagaaggaat taacacacga tcctctccgt taaagggttc ttcaacacca
     1561 ccattaaaaa aaaaggtaac atgggcatac ttttctgttt cggctaaacg tagttgtttt
     1621 aagccgttat cttgtagcac ttcgccaagc acttttggaa attgctccgg tgggtataaa
     1681 actttagcgg gcaatttggc atcatattcg gctaaagtta caaattcttc taaatttgga
     1741 aatttttttc tctcgaaatc tgtgaagttt ttatgggaaa gagcataact taattgtcta
     1801 gcgcgatcgg ctctaaaatt cataaataaa acacaatcac catcctggat cgtaataggt
     1861 tggtctgtat tggttatcac agtcggtttt acaaattcat cactttcccc acgttcatat
     1921 gccatcctta aaccttctaa tgcattcgta gctgcgtacc ctaatccttc ggttaacaga
     1981 tcgtaggcaa gttgggttct atcaaaacgt ttatcccgat ccatggcata aaagcgacca
     2041 caaatacttg caatttggcc aaacccatag ttttcaaacc aagtactcat tttttccaac
     2101 acggcataag cactttgcgg tgctgtatcg cgcccatcca aaaaagcatg gacgtatata
     2161 ttttttatat catgtttgcg cgcaaattct aaaacagcca taaaatgatc gctatgactg
     2221 tgcacgccac cttccgataa aagtcctagg atatgcaagg cgcgttgact agtctttacc
     2281 gtgttaaaaa gttctacgag aactggattt aaggaaaaat ttccggtttt aatgtcatgc
     2341 tgaattctat tcaggtcttg ataaataacc ctcccagcac ccatgcataa gtgaccaacc
     2401 tcagaattcc ccatttgtcc ttctgggagc cctacttctg cacccgatgc attgaggagc
     2461 ata
//
LOCUS       DASTIB010000016         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_997907, whole genome shotgun sequence.
ACCESSION   DASTIB010000016 DASTIB010000000
VERSION     DASTIB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997907"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFP93_00245"
     CDS             <1..284
                     /locus_tag="VFP93_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9843068.1"
                     /translation="LEVLKRSEILATHKIQKELLSVREKMGIILDTLRNKGIITFCAI
                     FSPDEGKMGVIVTFLAMLELVKESLIDIVQAEPFGVIHLKALDYEPTKE"
     gene            265..936
                     /gene="scpB"
                     /locus_tag="VFP93_00250"
     CDS             265..936
                     /gene="scpB"
                     /locus_tag="VFP93_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET9843069.1"
                     /translation="MNPPKNDTLTIIEGLLFAEGKPLSIEKLRALLPEEHRLSVGEIR
                     QLIEELKKLYENRGIELVEVASGYRFQIAPNVAQWLITQREEKPLRLSKAFMETLALI
                     AYRQPITRGEIEEIRGVVVNTNIIKNLLELEWIREVGYKDVPGKPALLATTKKFLDFF
                     NMKSLQELPPLAELQDLTELRLQEDLLESADIAQENTIDNKRAELTEIVMTHPLPRKA
                     IVHES"
     gene            926..1651
                     /locus_tag="VFP93_00255"
     CDS             926..1651
                     /locus_tag="VFP93_00255"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9843070.1"
                     /translation="MKVERLQKVLAQQGLGSRRKIEQWIEEGHFHVNGKIAHLGLKVS
                     SECNILFKGKAIQHKSFRPTLLLYHKPIGEVCSRKPDPHQKSIFDNLPPLMQGKWLSI
                     GRLDINTSGLLLLTNCGELAQRLMHPRYNNEREYRVRAFGKINPQILQNLKGETQLDD
                     GIAQCKHLNVVAQKGNNTWFSVVLSEGRNRIVRRLFEAHNLLVNRLIRVRFADFSLDK
                     NLKSGEYIYLDIPANLLRVSKQI"
     gene            1737..1988
                     /locus_tag="VFP93_00260"
     CDS             1737..1988
                     /locus_tag="VFP93_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843071.1"
                     /translation="MKNKKDDPNRPTPRKQSSIAKNLDENKDINKSYGLEREAAEYEM
                     EKNRGGLKKHLGKDREGEDLEKNAREKQEISSNPRGRSS"
     gene            2013..2174
                     /locus_tag="VFP93_00265"
                     /pseudo
     CDS             2013..2174
                     /locus_tag="VFP93_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
BASE COUNT          743 a          377 c          464 g          610 t
ORIGIN      
        1 ttttagaagt gttaaaacgt tccgaaattt tagctaccca caaaattcaa aaagaattac
       61 tttcggtgcg agaaaaaatg ggaatcatct tagatacttt acgaaacaaa ggtataataa
      121 ccttttgtgc aattttttcc ccagatgaag gtaaaatggg agttattgtt acttttttgg
      181 ccatgttaga actggtcaag gaatcgttaa ttgatattgt gcaagcagaa ccttttggcg
      241 ttattcattt gaaggctttg gattatgaac ccaccaaaga atgatacttt aacgattatt
      301 gaaggtttgc tctttgcaga aggcaaacct ctaagcatcg aaaagttgcg cgcgttgcta
      361 ccggaagaac accgcctttc tgttggagaa atacgccaac ttattgaaga gcttaaaaag
      421 ctatatgaaa acagaggtat tgaattggtt gaggtggcaa gcggatatcg ctttcaaatt
      481 gcaccgaatg ttgcgcaatg gttaattacg caacgggaag agaaaccttt gcgattgtca
      541 aaagctttta tggaaacttt ggcacttatt gcctatcgcc aacctatcac ccgaggtgaa
      601 attgaagaaa ttcgtggggt agtggtaaat accaacatta ttaaaaatct tttagaatta
      661 gagtggataa gagaagtagg ttataaagat gttcctggga aaccagcact ccttgccacc
      721 accaaaaaat ttcttgattt ttttaatatg aaatctttac aagagctgcc accccttgca
      781 gagcttcaag accttaccga attgcgactt caggaagatt tattagaaag cgcggacata
      841 gcgcaagaaa atacgatcga caataaacgg gcagagttaa cagaaattgt catgacccat
      901 ccacttccgc gcaaggctat cgtccatgaa agttgaaaga ttacaaaaag tattggcgca
      961 acaaggcttg ggatcacgtc gtaagatcga gcaatggatt gaagaaggcc attttcacgt
     1021 aaatggcaaa attgcgcatt taggcttaaa agtaagttca gagtgtaata ttttatttaa
     1081 gggtaaagca atacaacaca aaagctttcg ccccactttg cttctttatc acaagccaat
     1141 aggtgaggtt tgtagcagaa aaccagatcc ccaccaaaaa agcatttttg ataatttgcc
     1201 tcctcttatg caggggaagt ggctcagtat aggcagactg gatattaata catctgggct
     1261 gctcttgcta accaattgtg gcgaactagc tcaacgccta atgcaccccc gttataataa
     1321 tgaacgagaa tatagggtgc gtgctttcgg aaaaattaac ccccaaattc tgcaaaacct
     1381 caaaggcgaa acgcaattag acgatggtat agcacaatgt aaacatctta atgttgtagc
     1441 gcaaaaaggt aataatactt ggtttagcgt tgtgttaagt gaaggacgta atcgcatagt
     1501 acgacgctta tttgaagcac ataatttatt agtaaatcgt ttaattcgcg tgcgatttgc
     1561 ggattttagc ttggacaaaa atctcaaatc tggagaatat atttatttgg acattcctgc
     1621 taacttacta cgagtgagca agcaaattta ataacttatt acgttatatt tagccccacc
     1681 aattttattt tcataagcta aaattagatg agactctagc atgcattggg gaacttatga
     1741 aaaataaaaa agatgatccg aatcgaccaa caccgcgcaa gcaatcaagc attgcaaaaa
     1801 atttagatga aaataaagat attaataaaa gttatggttt agaacgagaa gcggctgaat
     1861 atgagatgga aaaaaatcgg ggcgggctaa aaaaacatct cggaaaggat cgcgaaggag
     1921 aggatttaga aaaaaatgct cgggaaaaac aagaaattag ttcgaaccct cgaggaagat
     1981 cttcataaac ggccctatta aggagaaaat acatgctgaa cttaggaacc attttactgt
     2041 tagtgttatt agtgatttta ataggagcga ttccggcttg gccctagagc cgtggctggg
     2101 gttattatcc tagtggtggt atcggattag ttttgcttat tgttttgatt ttggtattaa
     2161 tgggaagaat ttaatacaca aaaatgcatg acga
//
LOCUS       DASTIB010000017         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_669547, whole genome shotgun sequence.
ACCESSION   DASTIB010000017 DASTIB010000000
VERSION     DASTIB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669547"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFP93_00270"
     CDS             complement(<1..774)
                     /locus_tag="VFP93_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843072.1"
                     /translation="MILHGFFFIALVGILLTLLGITRLCLLGYDNEIIHWLFGDTKLL
                     SGLAFLLTGITFIITASKQHKLKNSVQYFSGIAIVILSCTFYLIKERIFNYENIYLYI
                     YEYGNTVQLSPAAFFCLSLLGLSLLLLPLGNRKSVGILLQVLAIVILIFALIAFCGYA
                     LKLGLLYNEYQYLTIPIISIFGLMLASCGLWNIYWKNKWFHNLYKGREDQKVMMTIAM
                     ISVCVISLSGFATYKTLSYENEKVIQQEFKNYLLVYKKQF"
     gene            895..2499
                     /locus_tag="VFP93_00275"
     CDS             895..2499
                     /locus_tag="VFP93_00275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9843073.1"
                     /translation="MSNAIEELKIAYLKSLPKKVQAMRELMEANHREAWSLLHRKAHS
                     LRGSAGTYGFENVSTAAATLEALLDKALKNDEDPRQEKLIWETFAILESSLHNSSNYS
                     LLNLPYSKEEKPHDIFCLLTDDSEIIHVIRESLNSLGFTIYEFTDSVEFENFVRMQAQ
                     YISLIDLQKVDPLLENFLKKNAQQVSHQKIVFTMDTDSDLSTRLRTIRFGSRAFFLKP
                     FHPGELIIKIEQIREASSQYRILILDDDPDTNQWYSFLLSNSGFHCKTLYSYEHIESE
                     MDEFKPDLVLMDMYMPEVSGIELAAVLRQRSVYEGIPIVFLSSEENKHEQIRAMRFGA
                     DDFIVKSHDPRHLIDSVYNRILRYKAIATKMVYDGLTAVYNHAFVMSRLEVECKLALR
                     LSTTLSIAILDLDNFKGINDTYGHPAGDHVLKTLCFLLRNRLRKSDIVGRYGGEEFIV
                     ILPHTKKEQAEVLLNVIKSHFNKITFSHENFQFTVSFSAGIAEFPREPNVESLIQSAD
                     EALYSAKNYGKNQVCVYNPFPIRKKG"
     gene            complement(2501..>2687)
                     /locus_tag="VFP93_00280"
     CDS             complement(2501..>2687)
                     /locus_tag="VFP93_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9843074.1"
                     /translation="HLEVRLHELDKNQAFVVLCRAGKRSLVAIEILKEHGFKNLVNLE
                     GGLYAWKTSVDPDFQLA"
BASE COUNT          936 a          478 c          454 g          819 t
ORIGIN      
        1 aaattgtttt ttgtacacta acaaataatt tttaaattcc tgctgaatga ccttttcatt
       61 ttcataggaa agcgttttat aggttgcaaa gcctgataac gaaataacgc aaacggagat
      121 cattgcgata gtcatcatca ctttttgatc ctctcgaccc ttatacaaat tatgaaacca
      181 cttatttttc caataaatat tccaaagacc acagctagca agcattaaac caaaaatgga
      241 aatgatagga attgttaaat actgatattc gttatataac aatcctaact ttaaggcata
      301 accacaaaaa gcaattaatg caaaaataag aattacaatg gctaaaactt gtaaaagtat
      361 gcctacagat ttgcgattac ccaagggtaa tagcaataaa gatagtccta ataaggacaa
      421 acaaaaaaaa gcagctgggc ttaactgaac ggtgttccca tactcataga tatataaata
      481 aatattttca taattaaaaa ttctctcttt tataagataa aatgtgcatg agagtattac
      541 aatagcaatt cctgaaaagt actggacact attttttagt ttatgttgtt tgctggccgt
      601 aataataaat gtaataccag ttaataaaaa tgccagccct gagagtaatt ttgtatctcc
      661 aaataaccaa tgaataattt cgttgtcata acctagaagg cataaacgcg taatacccag
      721 taaggttaat agaataccca ccaaagcaat aaagaaaaaa ccatgcaaaa tcatgtacat
      781 caaatcctaa atgtttatct caactatagc ctttcccatt ttaaaatagg tttatttgca
      841 catcttttgc tgcatatttt ctatacttaa aggatgctgg gaacaaagat ccttatgtcc
      901 aatgcaattg aagaattgaa aattgcttac ttaaaaagcc ttcctaaaaa agtacaagcc
      961 atgcgagagt taatggaagc taaccataga gaagcatggt ccttattaca taggaaagca
     1021 cattctttgc ggggcagtgc tggcacttat ggatttgaaa acgtttctac ggcagcagcc
     1081 acgctagaag cactattaga taaagcactt aaaaatgatg aagatccacg acaagaaaaa
     1141 ttaatctggg aaactttcgc aatcttggaa tcctctctgc ataattctag caattattca
     1201 ttgttaaacc ttccttatag taaagaggag aaaccgcacg acattttttg tcttttaacc
     1261 gacgatagtg aaataatcca tgtcattcgc gaaagtctaa attctctcgg gtttactatt
     1321 tatgaattta cggatagtgt agagtttgaa aattttgtaa ggatgcaagc gcaatatatc
     1381 agtttaatcg atctgcaaaa agttgatccg ttacttgaga atttcctaaa aaaaaatgct
     1441 caacaagttt cccatcaaaa aattgttttc actatggata ccgattctga tttatcaaca
     1501 cgtttgcgca ccattcgatt tggatctaga gcgtttttct taaagccgtt tcatcctggt
     1561 gaacttataa ttaaaattga acaaatacgt gaagcaagct cacaatatcg aattttaatt
     1621 cttgacgatg atccagatac caaccaatgg tatagttttc tcctatccaa ttcaggattt
     1681 cattgcaaaa cgctatattc ttatgaacat attgaatcag agatggacga attcaaaccg
     1741 gatcttgtat taatggatat gtacatgcca gaagtgagcg gtattgaatt ggctgcagta
     1801 cttagacagc gctctgtgta tgaaggtatt cccatcgttt ttttatctag tgaagaaaat
     1861 aaacatgaac aaatcagggc catgcggttt ggtgctgacg attttattgt gaaatctcat
     1921 gatcccagac atttaattga ttctgtatat aatagaattt tgcgctataa ggcgatagct
     1981 acgaaaatgg tatacgacgg tcttaccgct gtttacaacc acgcctttgt aatgagtcgt
     2041 ttagaggtcg aatgcaaact agcgctgcga ttaagtacaa cgctcagtat cgcaatatta
     2101 gatttagata actttaaagg aattaatgat acttatggtc atccagctgg cgatcatgtt
     2161 ctgaaaacct tatgctttct tttacgcaat cgtttgcgaa aatccgatat agtaggacgt
     2221 tatggtggag aagaatttat tgtaatttta ccccacacaa aaaaagagca agcggaagtc
     2281 ttacttaatg taataaaatc acacttcaat aaaattactt tttcccatga aaattttcaa
     2341 tttacagtaa gttttagtgc cggtatagca gaatttccta gagaacccaa tgtggaatct
     2401 ctcattcaat ccgcggacga agcactttat tctgcaaaaa attatgggaa aaatcaggtt
     2461 tgcgtgtata atccatttcc gatacgaaaa aaaggataat ttaggcgagt tgaaaatcgg
     2521 gatcgacaga agttttccaa gcgtataagc caccttctaa gttcactaag tttttaaaac
     2581 cgtgttcttt taaaatctca atggcaacaa ggcttctttt accggcacga cataaaacga
     2641 caaaggcttg attcttgtcc aattcgtgta atcgcacctc taaatga
//
LOCUS       DASTIB010000018         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_923045, whole genome shotgun sequence.
ACCESSION   DASTIB010000018 DASTIB010000000
VERSION     DASTIB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923045"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..876)
                     /locus_tag="VFP93_00285"
     CDS             complement(40..876)
                     /locus_tag="VFP93_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HET9843075.1"
                     /translation="MQCRNFLVFGALIAGTAVAQPDASGSSDYQGDRQVFEQYSDMNP
                     FAFSLYKSNYVLPFSHSSNVTNPIIPFPERETKHTELVFQLSFKLPLFSSLFTEKTKL
                     YFAYTQLTFWQLYTEDPFIRETNYEPEIFITHQMNRCSKFTLGMGHESNGRGGLFERT
                     WNRLYLDYHVDLNPYFWFSAQPWLIVFKSNSSSEHNPDIGHFLGYDQFIFGLNVGRFI
                     ANLRLSNIESGFSRGQRELNVGYRMGKWHLFGQVFSGYGHSLLEYNHSTTAFGLGIAL
                     NV"
     gene            complement(979..1839)
                     /gene="fghA"
                     /locus_tag="VFP93_00290"
     CDS             complement(979..1839)
                     /gene="fghA"
                     /locus_tag="VFP93_00290"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HET9843076.1"
                     /translation="MTIQLIEQHKCFGGTQAIYSHLSTENTCEMRFGIFIPPQAESRK
                     VPVLYWLSGLTCTEENFITKAGAQRIAAELGLIIVVSDTSPRNLGIPGEEEFSDFGTG
                     AGFYVDATQKPWSKGYRMYSYICKELTSLVELNFNADPSRVGIFGHSMGGHGALMIAL
                     KNPHYFKSVSAFAPICSPIHSPWGIKAFTGYLGSSTDQWEQYDATRILEEIGWKWSTL
                     FIDQGTCDPFLKTQLKPEILIESCEKANVSLKLRMQEGYDHSYFFVTSFIEDHLRFHA
                     ENLLGLHKAA"
     gene            complement(1836..>2376)
                     /locus_tag="VFP93_00295"
     CDS             complement(1836..>2376)
                     /locus_tag="VFP93_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017651796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9843077.1"
                     /translation="VFGLGGIGLNVIQGAKMVGANRIIGVDINPSKKILAEKLGMTHF
                     VNPTEIQEDLVPFLINLTNGGADYSFECVGNVLLMRQALEVCHKGWGVCTIIGVAGAG
                     QEIATRPFQLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKIHIDDFITHQFPLEQIH
                     DAFDLMHAGKSIRSVVTFS"
BASE COUNT          776 a          507 c          419 g          674 t
ORIGIN      
        1 taatagaaga ttattttagg tactttgaaa aaatcagtat tacacattta aagcaattcc
       61 tagtccgaag gcagtcgtag aatgattata ttcaagcaaa ctatggccat accctgagaa
      121 aacttgtcca aagagatgcc attttcccat tcgataaccg acgtttagct ctctttgtcc
      181 tcggctgaac ccgctttcta tattacttaa gcgcaaatta gcaatgaatc ttcctacatt
      241 caaaccaaag atgaattgat catagccaag aaaatggcct atatcaggat tgtgctcact
      301 ggaagaattg ctcttgaaca caatgagcca tggttgggcg ctaaaccaaa aatatgggtt
      361 taaatctaca tggtagtcca aataaagtct attccacgtt ctttcaaaaa ggccgcctct
      421 accgttagat tcatgcccca ttcctaatgt aaattttgaa caccgattca tttgatgcgt
      481 gataaaaatt tcgggttcat aatttgtttc acgaataaaa ggatcttctg tataaagttg
      541 ccagaaggtc aactgggtat acgcaaaata aagtttggtt ttctcggtaa aaagactgct
      601 gaacaatggt aacttaaaac ttaactggaa aactaactct gtatgcttcg tttcacgttc
      661 cggaaagggt attataggat tggtaacatt cgatgaatgt gaaaagggaa ggacataatt
      721 cgacttataa agcgagaatg caaagggatt catgtcagag tattgctcaa aaacttgacg
      781 atctccttgg taatccgacg atccagatgc atccggttgg gctacggctg taccagcaat
      841 cagcgcacca aaaaccaaaa aatttctaca ttgcatcgtt aaatcctttt agcagtaaat
      901 taacagactg aaggctatca ataaaataac tataacatac tcaatattag tttatttgct
      961 aaaagcaaaa gtaaaaaact aagcggcctt atgaagaccc aacaaattct ctgcatgaaa
     1021 ccgtaaatga tcttcgataa aacttgtaac aaagaaataa ctatgatcgt agccttcttg
     1081 catgcgcagc tttaaagata cgttggcttt ttcacaagac tcaataagta tctcaggctt
     1141 aagctgtgtc tttaagaaag gatcacacgt tccttgatct ataaataaag tagaccattt
     1201 ccagccaatt tcttcaagaa ttcgtgttgc atcatattgt tcccattgat cggtactact
     1261 tcctaaataa ccagtaaaag cttttatgcc ccagggcgaa tgtataggtg aacaaatggg
     1321 agcaaaagca gatacagatt taaagtagtg tggattttta agtgcaatca taagtgctcc
     1381 atgacctccc atcgaatgac caaaaatacc tacgcgggag ggatcagcat taaaatttaa
     1441 ctctacgaga gaggttagct ctttgcaaat ataagaatac atacgatatc ctttcgacca
     1501 aggcttttgg gtagcatcaa cataaaatcc tgcacctgta ccgaaatctg aaaactcctc
     1561 ttcaccaggg atgcctaaat tgcgtgggct tgtatcagaa acaacaataa ttaagcccaa
     1621 ttcagctgca atacgctgag ctccggcttt ggtgataaaa ttttcttctg tgcaggttaa
     1681 gcccgagagc caataaagca caggcacctt cctggattct gcttgcggag gaataaaaat
     1741 accaaaacgc atttcgcatg tattttctgt agataagtgg ctataaatag cttgtgtacc
     1801 accaaaacat ttatgttgtt caataagttg gatcgtcatg aaaaagttac cacgcttcta
     1861 atcgatttac ctgcatgcat taaatcaaat gcgtcatgaa tttgctctaa aggaaattgg
     1921 tgtgtgataa aatcatcgat atgaatcttt ccttccatat accaatcaac aattttaggc
     1981 acatcggtgc gccctcttgc tcccccaaag gcagagccct tccacacacg tcccgtaacc
     2041 agttgaaaag gtctagtcgc tatttcttgt cctgctcctg caacgccaat aatggtgcac
     2101 acgccccacc ctttgtgaca tacctctaaa gcttgacgca ttaatagcac attaccaaca
     2161 cattcaaaac tataatcagc gccgccgttg gtcaaattaa ttaaaaatgg caccaaatct
     2221 tcttgaattt ctgtcggatt cacaaaatgc gtcattccga gcttttcagc gagtattttt
     2281 ttgctagggt taatatctac tccaattatc ctattagcac ctaccatttt ggcgccctga
     2341 ataacattta agccaatacc acccaaacca aacaca
//
LOCUS       DASTIB010000019         4163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1041911, whole genome shotgun sequence.
ACCESSION   DASTIB010000019 DASTIB010000000
VERSION     DASTIB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041911"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..967)
                     /locus_tag="VFP93_00300"
     CDS             complement(158..967)
                     /locus_tag="VFP93_00300"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HET9843078.1"
                     /translation="MATYAIGDVQGCFRALQSLLEKIGFNPQQDTLWFVGDLVNRGPQ
                     SSEVLRYIKNLEDRAITVLGNHDLSLLALSYGIIESKPKDTYQTVLEEPDAKELLHWL
                     KSQSLMHFDAQLNYACAHAGIFPLWDINSAQIFASEVETILRSTHSEVFLRNMFGKEP
                     VAWDPHLTGWSRLRFITNAFTRMRFCTLHGELNLTAKGGLSKCPSDCVPWFHYAKPAW
                     LGCNIVFGHWAALNGETHRDSIQALDTGCVWGNCLTAFCLETKQRFQIECQ"
     gene            complement(967..1347)
                     /gene="apaG"
                     /locus_tag="VFP93_00305"
     CDS             complement(967..1347)
                     /gene="apaG"
                     /locus_tag="VFP93_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET9843079.1"
                     /translation="MDLQAHKIDISVITKYLPEQSAPAEFRFAFSYTITIFNAGNIAA
                     LLMRRHWEITNANGEVREVDGKGVVGEQPHLQPGESFRYTSGAIIDTPVGSMQGTYTM
                     LADDGTEFLAHIPAFRLAMPEIVH"
     gene            1507..1767
                     /locus_tag="VFP93_00310"
     CDS             1507..1767
                     /locus_tag="VFP93_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843080.1"
                     /translation="MRDLTINDLQLVNGGATKAEIIGDAAVYGGALGLVGGGVAGAAI
                     GAAFFGPIGAVLLTVPSAMCGFATGVMVLPVLSFPVAAIFGK"
     gene            complement(1853..2629)
                     /gene="rsmA"
                     /locus_tag="VFP93_00315"
     CDS             complement(1853..2629)
                     /gene="rsmA"
                     /locus_tag="VFP93_00315"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9843081.1"
                     /translation="MSPKKHLGQHFLSDPYIVEQIIRSMHIREEDRIVEIGPGQGALT
                     RQLLKLCNELYAIEYDADLIPYLQNKHPNLNLFHQDALTFDFQQLATEKKLRIIGNLP
                     YNISTPLIFHFLKYSEHIVDMTLMLQKEVAERICANPGSKQYGRLTIMTQYHCSVEKI
                     LDVPDTAFFPPPKVQSAVIRLQPFTQENNPFPNISLEVLSKIVTEAFSHRRKTLRNAL
                     KLFLTDTTLEKLDIDPQARPETLTIAQFISIAKILQKTDF"
     gene            complement(2608..3624)
                     /gene="pdxA"
                     /locus_tag="VFP93_00320"
     CDS             complement(2608..3624)
                     /gene="pdxA"
                     /locus_tag="VFP93_00320"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET9843082.1"
                     /translation="MIPLKRIAVTVGEPGGVGPDLILNLAQRPFPGEVVVFGCPKNLH
                     YRAKVLNLPIKFVEADFLQPPQQNGNGLIRLYPIPLPKPQVLGYADNVNRLQVLESLK
                     TAFSFCMEGLTQAIVTGPVQKSALNTLTEPFYGHTEFFADLAQTKNILMAFYHPKCIV
                     GLLTTHIPLKLVPERITQERLTQAIHLLNTYLNRWFSRPSPRIIVLGLNPHAGEQGLF
                     GTEEIEIIAPVIYDLQQQGINVHGPVSADTAFTPNTLDQADAILALYHDQGLTPLKSF
                     GFGNIVNITMGLPILRASVDHGTAFALAGTKHVDTASFSSALQLAFALINQQEMHLCH
                     PKNI"
     gene            complement(3611..>4163)
                     /locus_tag="VFP93_00325"
     CDS             complement(3611..>4163)
                     /locus_tag="VFP93_00325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9843083.1"
                     /translation="GDIRGPIRSPSGFHVVKLVDKRASDAKNLSLQKTQVRHILIKTN
                     ALLSDDEARYKLRDFKRELESGTDFAIIAKTHSEDLASANQGGDLGWVTSSQLVPEFS
                     AVMDKTAIGGISDPFNSPFGWHILQVLDRKEEKNSEAILKQNAMNMIKNRKAEEKLEN
                     WLRQMRDEAYIQVHLPGYTDDPA"
BASE COUNT         1211 a          803 c          863 g         1286 t
ORIGIN      
        1 aaggacatgc cattgctctc catacggcca atttatttat ttgatttaat aagatagtgc
       61 gagctattcg ttagcgttgc tgggtcgtac tttaccgcga ttcctgaaag ataggaatag
      121 ccagttacca actaagcaca tcacgtttag ggttgattta ctgacattca atttggaatc
      181 tttgtttagt ttcaagacaa aatgcagtta aacaattgcc ccaaacacac cccgtatcta
      241 atgcctgaat agaatcccta tgggtttctc cgttcaaggc tgcccaatgc ccaaaaacaa
      301 tattacatcc aagccacgcg ggttttgcat aatgaaacca cggcacacaa tcacttgggc
      361 atttacttaa accaccttta gcagttaaat tgagttctcc gtgtagggtg caaaaacgca
      421 tgcgcgtaaa agcattggta ataaagcgta gtcttgacca cccggtcaga tgtggatccc
      481 acgcaacagg ttctttgcca aacatattgc gcaaaaaaac ttcactgtgc gtacttcgta
      541 aaatggtttc tacttcagag gcaaagattt gcgctgagtt aatatcccac aggggaaaaa
      601 tgccagcatg cgcacaagca taatttaatt gcgcatcgaa gtgcattaag gattgcgatt
      661 taagccaatg taatagttct tttgcatccg gctcttcgag aacggtttga taagtatctt
      721 ttggctttga ttcaatgatg ccataagata aagctaataa agacaaatcg tgattcccca
      781 ataccgtaat agctctatcc tcgagatttt taatgtagcg taaaacttcg ctggattgtg
      841 ggccacgatt aaccagatcg cccacgaacc acaaggtatc ttgttgtgga ttaaaaccaa
      901 ttttttctag caagctttgt aacgcgcgaa aacaaccttg tacatcgcca attgcatacg
      961 ttgccattag tgcacgattt caggcatagc taatcgaaag gcagggatat gggccaaaaa
     1021 ttctgtgcca tcgtctgcaa gcatggtata agttccttgc atgctcccca caggggtatc
     1081 aataattgca ccgcttgtat aacgaaaaga ttctcctggt tgtaaatggg gttgctctcc
     1141 taccacacct ttaccgtcta cttcgcgaac ttcaccgttg gcattggtaa tttcccaatg
     1201 tctgcgcatt aaaagagccg caatatttcc cgcattaaaa atagtaatag tgtaggaaaa
     1261 ggcaaaacgg aattctgctg gcgcagactg ctccggcagg tatttagtta tgacggatat
     1321 gtcaatttta tgcgcctgta aatccatgga ttctcctgta ctgatagcgt aagcataaaa
     1381 tgggttagca acacaggcaa gttaaaatca ttacttcctt atgtgttcag ctaactttaa
     1441 attataaaac ttgacggata actaaaattt tcataggatc cattttttag aattattgag
     1501 attattatgc gtgatttaac tataaatgac ttacaactag tcaatggtgg agcaactaaa
     1561 gctgaaatca ttggagacgc tgctgtgtat ggtggtgccc ttggtcttgt aggtggcggt
     1621 gtcgcaggtg ctgcaattgg agctgccttt tttggtccta taggtgctgt tctgttaacg
     1681 gttccaagtg ctatgtgtgg ctttgcaact ggggtgatgg tactacctgt attgagtttc
     1741 cctgttgcag caatctttgg taagtaacat catttaataa ttttgctgtc attataaggg
     1801 atatatgtca gattaaacct gaatagtttt tgtaatgaca gtttttttat tgttagaaat
     1861 ccgttttttg aagaatttta gctattgata taaattgagc aatagttaat gtttctggtc
     1921 gtgcttgcgg atctatatcc aacttttcca gagtagtatc tgtcaaaaaa agtttcaaag
     1981 cattgcgtaa ggttttacgc cgatgcgaaa aagcttcggt aacaattttg cttaacactt
     2041 ctaacgaaat attcggaaag ggattgtttt cttgcgtaaa aggctgcaag cgtataactg
     2101 cagactgaac tttgggtggc ggaaaaaaag cagtatccgg tacatcgagt attttttcga
     2161 cggaacaatg atattgcgtc ataatggtta ggcgcccata ttgtttagag cctggattcg
     2221 cacaaatgcg ttctgcaact tctttttgta acattaaagt catatctaca atatgttcac
     2281 tatattttaa aaagtgaaaa atcaaaggag ttgaaatgtt ataaggcaag ttgcctatga
     2341 tacgcaattt tttttccgtt gctaattgct gaaaatcaaa cgttaatgca tcttgatgaa
     2401 ataagtttaa gttaggatgc ttgttttgca aataggggat taaatctgca tcgtattcaa
     2461 tggcatacag ctcattgcac agttttaaca gttgacgcgt gagtgcgcct tggcctggtc
     2521 caatttcaac gatcctatcc tcttctctta tatgcataga acgaattatt tgttcgacaa
     2581 tgtaaggatc cgataaaaaa tgttgaccta aatgtttttt gggtgacata aatgcatttc
     2641 ttgttggttt attaaggcaa aagccaattg aagagcagag ctaaaacttg cagtatctac
     2701 gtgtttagtt cccgcaagtg caaaggcagt accatgatca acggacgcac gaagtatggg
     2761 taatcccatg gttatgttta caatattgcc aaaaccaaag cttttaagag gcgttaagcc
     2821 ttgatcatga tacagggcca aaatagcatc ggcttgatct aacgtattgg gtgtaaaagc
     2881 cgtatctgca gaaacagggc cgtgcacatt tatgccttgc tgttgcaaat cgtaaattac
     2941 aggcgcaata atttcaattt cttctgtacc gaataaacct tgctcacctg catgtggatt
     3001 caatcccaac acaataatac gggggctagg tcgcgaaaac catcggttta agtaagtatt
     3061 cagtaggtga attgcctgcg ttaaacgttc ttgcgtgata cgttccggaa ctagttttag
     3121 tggaatatgg gtggttaata agcccactat acatttggga tgataaaaag ccattaaaat
     3181 attttttgtt tgtgctaaat ctgcaaaaaa ttctgtgtgg ccataaaaag gctctgttag
     3241 ggtattaagt gcgctttttt gtactggacc tgtgacaatg gcttgcgtaa gaccttccat
     3301 gcagaaggaa aaggcggttt taagactttc taatacctga agtcggttta cattatcggc
     3361 atagcctaac acttggggtt ttggtaacgg gatcggatat agcctaatta aaccgttacc
     3421 attttgttgg ggaggttgaa gaaaatcggc ctcaacaaat tttatcggca gatttaacac
     3481 tttggcgcga taatgtaaat ttttggggca accaaaaact acgacttcgc cgggaaaagg
     3541 tcgctgtgct aaattcagaa ttaaatcagg gccgacacca cctggttctc caaccgttac
     3601 agcaatacgt ttaagcggga tcatcggtgt agccgggtag gtgaacttga atataagcct
     3661 catcgcgcat ttggcgtaac cagttttcaa gtttctcttc ggctttgcga ttttttatca
     3721 tattcatggc attttgtttt aaaatggctt cagaattttt ttcttctttc ctatccaata
     3781 cttgcaaaat atgccaaccg aaaggtgaat taaatgggtc actaattcca ccaatagcag
     3841 ttttatccat cactgccgaa aattctggca ctagttgcga agaggtgacc caacctaaat
     3901 caccgccttg gtttgcagaa gccaaatctt ctgaatgcgt ttttgctatt atggcaaagt
     3961 cggtgccgct ttctaattca cgtttaaaat cacgtaattt atatctggcc tcatcatcag
     4021 ataacaaagc atttgttttt atgagaatat gacgcacctg agttttttgt aaacttaagt
     4081 tttttgcatc ggatgcacgt ttatctacta acttgacaac atgaaatcca ctcggactac
     4141 gaataggacc acgtatatca cct
//
LOCUS       DASTIB010000020         5387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1473962, whole genome shotgun sequence.
ACCESSION   DASTIB010000020 DASTIB010000000
VERSION     DASTIB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473962"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VFP93_00330"
     CDS             complement(<1..1290)
                     /locus_tag="VFP93_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843084.1"
                     /translation="MKNKTGSNENKQTGANDEANELDTLLSKLEEDRRSSANKKKTVK
                     KNLTKAKSKVALDELNKLMEDFEVKQKSHTKEKSTDEKWNEIEASQKNLLEELEAINL
                     EIEDNSLNITQTPEEKKQEKAKNSEIDALDAKQYINSLLNNTRIFTQDERYDHRALMR
                     TKAFDEIFKKLKHDGANLALLINFLVQDKKQNSGLEGELANFIIELLRADMQKTLDSG
                     NLNQTSFEYFFSDKDIDEAQLFTNFPNYNKQFEDVLKQRSDNLQNKLATKQDQFQVEI
                     KERLNQEAIEKQAPTSEANPEENSINILDELLDELTENNPKINPDESVKDNKPQAATE
                     DKNSAPLTENSLVSEMAQEEYSFESLIKPAEDIVKEEAIENTKSKMPTFLKDNVKEEL
                     FKSLNAKDFKGLYNNLILHKENLESLTNSMVADRENNP"
     gene            1556..2317
                     /locus_tag="VFP93_00335"
     CDS             1556..2317
                     /locus_tag="VFP93_00335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9843085.1"
                     /translation="MSAQNLKISIITACKNAYPDLQKTIKSIQSQSYNFIEHVIVDGD
                     STDGTAKYLNHMNLANTKWLSEKDKGISDAFNKGIQLASGDYLYFLGAGDTLYQKDIL
                     TKIFTTLRTRPLLICGKVMRTNAQGKSLWIAPQKWPTQFNKRSLLYKLSLPHQGLFTH
                     KSYFERFGLFSLKCKFAMDYEIILRSYHNFPEIQLVSDIVATWQAGGIGHNRTLEIYD
                     EYLRIKLQHQIASRWRLIVIDRWNRLKFTLKNLVG"
     gene            2423..>5387
                     /locus_tag="VFP93_00340"
     CDS             2423..>5387
                     /locus_tag="VFP93_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843086.1"
                     /translation="MSLVSNLKKGLYFAALSSTLFFPPQSSALLNVNTQVGDSDTFPP
                     TLGYLFLLPNSTFTILDDDDLGNTLGVSAANFITATNNALNFAGTSIVSGNIGASLLD
                     TPFAAINLQGEGETVTFQGDLLLTDALNFSANTIANVADNIIITGVVDNISGLPNVGQ
                     LNFLGSGTVVGSIGITDSLNHIEINTLKTAGEELILEGTQVNVGSIHLNDDGLNPTIL
                     TLNNSNLAFNGNITVETSNLNILNVENASTLKGNVGQTGAIFNLVQVAENSDLTYTGK
                     MFANLLEFQNNNTLFLGKNSIINGDIDTKLLGSGTLTFLNSGEVHGDVGQINAIHVSG
                     NLGTTALLDGIINTDINFTLGAAGPSFLVLDNNTQVLGNIDSTVGPNIGAIGFVGNAL
                     VQGTIGANNAVNEILLTEAGSTIVFNNNVTVGAGNFNFFADNKAQFAPNVILDANVNN
                     FSGFDNQGNLYFTGSGTVTGDIGATSPLNTITVEENGIGEILTLTGTTINTKTIHLKN
                     TTDTPITILLDNPLMQINAHFTVGSDDVNVIDAQKVLSITGDIGALNAGFNLIKIAQN
                     NDLNYSGNIIATTTQFQGNNNFNLADDSFIQGNVVTTMLNTGTLNFLGNGGVEGEIGE
                     LAAVNIMGGANTQVRLQGTINAGTVQFAGGAVTSTELILEDGSRINGTLDNIVGPDVG
                     SLTLEQNAEIIGSIGATNPLYTITLEGDGAAIAKFYDSVQVGSGNINFSGNATLEMVS
                     DTLDASIDNISGIAGFGNISFLTSGTITGNIGATNPLNVITVNLLEIPFANLELTGDI
                     VRSDIILHDDGLTPTTLLLNNPNMDFMGNIDVNTHNLNSMNVKQAISLTGEIGASTGF
                     VKLLKIGENQDLIVNGDIYADNIEFQNDNKLFLQEGNLHGKVTTLVNNGGSVIFGSNS
                     TIAFPMGVGLPLNTVEINANQKTVVLNNTIFANNTNIAAGTLQVNSPQSITGDLNVQN
                     QGILSLGTGANPLLVN"
BASE COUNT         1634 a          901 c         1014 g         1838 t
ORIGIN      
        1 aggattattt tcgcgatctg caaccattga atttgttaag gattccaaat tttctttatg
       61 aagaattaaa ttgttgtata gacccttgaa atcttttgca tttagtgatt taaaaagctc
      121 ttcttttacg ttatctttca gaaacgtggg catttttgac tttgtatttt ctatagcttc
      181 ctcttttact atatcctcgg ctggttttat tagactctca aatgaatatt cttcctgcgc
      241 catttcagat actaagctat tttcggttaa tggtgccgaa tttttatctt cggttgcagc
      301 ttgaggctta ttatccttta cggattcatc tgggtttatt ttcggattat tctcagtaag
      361 ctcatctaat aattcatcta gaatatttat cgaattctct tccggattcg cttctgatgt
      421 aggagcttgc ttctctattg cttcttgatt taatctttct tttatctcta cttgaaattg
      481 atcttgtttc gtagccaatt tgttttgcaa gttatcagat ctttgtttca gaacatcttc
      541 gaactgcttg ttgtaattag gaaaattagt gaataactgt gcttcgtcaa tgtctttatc
      601 actaaagaaa tattcaaatg atgtttgatt caagttgcct gaatctaatg ttttctgcat
      661 gtcggctctg agtaattcaa taataaaatt tgctagttca ccttctaaac cggaattttg
      721 ttttttatcc tgaaccagga agtttattag tagcgctaga ttagcgccat catgtttaag
      781 ctttttaaag atttcatcaa aagctttagt tctcattagc gcacgatggt cataccgttc
      841 gtcttgtgta aatatgcgag tattattcag caaactgtta atgtattgtt tagcatcgag
      901 tgcatctatt tcggaatttt tagctttctc ttgttttttt tcttctggag tttgagtgat
      961 attaagagaa ttatcttcga tttcaaggtt aattgcttcg agttcttcaa gcaaattttt
     1021 ttgtgaagcc tctatctcat tccacttttc atctgtggac ttttcttttg tatgtgattt
     1081 ttgcttgact tcgaaatcct ccatcaattt gttcaattca tctagtgcaa cttttgattt
     1141 agctttagtt agattctttt taacagtttt cttcttattg gcactagatc gacggtcctc
     1201 ctcaagcttc gataataggg tatcaagttc attagcttca tcattagcac cagtctgctt
     1261 attttcgttt gagcccgttt tattcttcat gccaaacctc ttaagaggaa gaattacctc
     1321 tttatatatt atcaaactaa cttagctggg gcatctatta tttcaaaaaa agtaattaaa
     1381 atttcattta taaccttttg aatattttaa gaaaaatttt tcgtgcccta gaaaaaattt
     1441 ttatttagat tgattcgtaa taggtctctt cgttcaattt tattaaatga acggtaaact
     1501 aggtttttgc caaaaatttg tgtagttgcg ctccgattaa tgcttaaggt aatttatgtc
     1561 agcacaaaat ctaaaaatta gtattattac cgcgtgtaaa aatgcttacc ccgatctgca
     1621 aaaaacaata aaaagtattc aaagccaaag ctataatttt atcgagcatg ttattgtcga
     1681 tggggattct acggatggaa ctgcaaagta tttaaaccac atgaatcttg ccaacacaaa
     1741 atggttaagc gaaaaggata aaggtattag tgatgctttc aataagggga ttcagctggc
     1801 atcaggagat tatttgtatt tcttaggagc aggagatacg ttgtatcaaa aagatatttt
     1861 aacgaaaata tttacaactt tgcgtactcg acccctattg atttgcggca aagtaatgcg
     1921 aaccaacgca caagggaaat cgctatggat agctccccaa aaatggccaa cacagtttaa
     1981 taaacgttca ttactttata aactttcctt gccgcaccaa gggttattta cccataaaag
     2041 ctattttgaa cgtttcggat tatttagctt gaaatgtaaa tttgcgatgg attatgaaat
     2101 tattttgcgc tcttatcaca attttcccga aatacaactc gtttcagata ttgtcgcaac
     2161 ctggcaagct ggcggtatag gacacaatcg tactttagaa atttatgatg aatatttgcg
     2221 tataaaatta caacatcaaa tagcatcgcg ttggcgttta atcgtaattg atcgatggaa
     2281 taggctaaag tttacgctaa aaaatttggt cggataagga gaatatagat tattttgcaa
     2341 aaaatataat cacaaactaa gtagctttac ttaatcatta atctgttatt agatcttact
     2401 atgcttttct gtggatcatt ttatgagctt agtatctaat cttaaaaaag gactttattt
     2461 tgcggcgtta agctcgaccc ttttttttcc accacaatca agtgcgctac tcaatgtaaa
     2521 tactcaggtg ggagattccg atacttttcc tcctacgctt ggctatcttt ttttattgcc
     2581 caattctact tttactatat tagatgacga tgatttaggg aatacactag gcgtttctgc
     2641 ggcaaatttt ataactgcta ccaataatgc attgaatttt gcgggaacct ccattgtatc
     2701 tggaaatatc ggtgcaagtc ttttagatac cccatttgct gcgattaatt tacaaggaga
     2761 aggggagact gtcacctttc aaggagattt actgttaaca gacgctttaa atttttcagc
     2821 aaacacaatc gccaacgtgg ctgacaatat tataataacc ggtgtggtag ataatatctc
     2881 aggtttgcca aacgttggac aactgaattt tttaggatct ggcaccgtcg tgggcagtat
     2941 tgggataaca gattctctta atcacattga aattaatacc ttaaaaactg cgggtgaaga
     3001 gcttatttta gagggtacgc aagtcaatgt gggatcgata catctcaatg atgacggact
     3061 caatcctacg atattaactt taaataatag caatttagcg tttaatggta atattacagt
     3121 cgaaacttcg aatctcaata ttttaaatgt agaaaatgct agcaccctaa aaggcaatgt
     3181 agggcaaaca ggtgcgattt tcaatcttgt gcaagtggca gaaaatagcg atttaacgta
     3241 taccgggaag atgtttgcta atttgctgga gtttcaaaat aataatacgt tatttttggg
     3301 aaaaaacagt atcattaatg gcgatattga tacaaaatta cttggatcag gaacgcttac
     3361 ttttttaaat agtggggaag tgcatggtga tgttggccaa atcaatgcca ttcatgtttc
     3421 aggtaatctt ggtacgacag cacttttaga tggcatcatc aataccgata ttaattttac
     3481 gctaggtgct gcaggcccta gcttcttagt gttagacaac aatactcaag ttttaggcaa
     3541 tatcgatagc acggttggtc ctaatattgg agctattggt tttgtaggca acgctttggt
     3601 acaaggtact attggggcta ataatgctgt taatgaaatt ttattaaccg aagcaggcag
     3661 taccattgtc tttaataata atgtaacggt tggtgctggc aattttaact tttttgcgga
     3721 taataaagcc caatttgctc cgaatgttat tctcgatgcg aatgtaaata atttctcggg
     3781 ttttgataac caagggaatt tgtattttac aggttcaggt acagttaccg gggatattgg
     3841 tgctacgagc cctttaaata ctattaccgt agaagaaaat ggtattggag aaattttaac
     3901 gcttactgga actaccatca acaccaagac catacattta aaaaatacca ccgatacccc
     3961 gattactatt ttgctggata atccattgat gcaaatcaat gcacatttta ccgtaggtag
     4021 cgacgatgta aatgttatag atgcgcaaaa ggtgctgagc attacaggtg atataggagc
     4081 attaaatgct ggctttaact taataaaaat tgcgcaaaat aacgatttaa attattccgg
     4141 aaacattatt gcaactacta cccaatttca aggtaataat aattttaatt tagcagacga
     4201 ttcctttatc caaggcaatg ttgtcacaac gatgttaaat actggaacct taaacttttt
     4261 gggcaatggt ggtgttgaag gagaaattgg cgaacttgca gcggtgaata ttatgggagg
     4321 cgcgaatacg caagttcgtt tacaaggcac aatcaatgca ggaacagtgc aatttgctgg
     4381 tggtgcagta accagcaccg aattgatttt agaagatggc tcgcgtatta atggcacgct
     4441 ggataatatc gttggtccag atgttgggag cttaactctt gaacaaaatg cggaaataat
     4501 tgggtctatt ggcgcaacta accctctata caccattaca ttagaagggg atggagcagc
     4561 tatcgcgaaa ttttacgaca gtgttcaggt gggtagtggg aacattaatt tttccggaaa
     4621 tgcaacgctt gagatggtga gcgatacttt agacgcaagc atcgataata tttcaggcat
     4681 tgcaggcttc ggtaatattt cttttttaac ttccggcact attacgggta atattggcgc
     4741 tactaatcca cttaatgtaa ttacggtaaa cttactggaa attccttttg caaatttgga
     4801 actaacaggt gatatagtac gttcggatat aatattacat gacgatggtt taaccccaac
     4861 aaccctgtta ttgaacaatc caaatatgga ttttatgggc aatattgacg ttaatacgca
     4921 taatttaaat agtatgaatg taaagcaagc aatttccctt acaggagaga tcggtgcttc
     4981 aactggattt gtgaagttac taaagattgg tgaaaatcag gatttaatag taaatggtga
     5041 tatttatgct gataacatcg aatttcaaaa tgataacaaa ctctttttac aagaaggaaa
     5101 cttacacggc aaagtaacta cgttagtgaa taatggtggt tccgtgattt ttggatcgaa
     5161 ttctactatt gcattcccca tgggggtggg tttaccttta aatacagtag aaatcaatgc
     5221 aaatcaaaaa actgtggtgt taaataatac aatttttgca aataacacaa atattgctgc
     5281 aggtactttg caggtcaatt cgccacaaag tataacgggt gatttgaatg tacaaaatca
     5341 gggtatatta agcttaggta ctggggcaaa tccattgctc gttaatg
//
LOCUS       DASTIB010000021         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_982563, whole genome shotgun sequence.
ACCESSION   DASTIB010000021 DASTIB010000000
VERSION     DASTIB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982563"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1180)
                     /gene="ribB"
                     /locus_tag="VFP93_00345"
     CDS             complement(38..1180)
                     /gene="ribB"
                     /locus_tag="VFP93_00345"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET9843087.1"
                     /translation="MHTTKELIEDIKQGKLVILMDDEDRENEGDLVIAAEKVKEEHIN
                     FMAQFARGLICLPMSKQHCERLHLPLMVPLNNQSKFGTKFTVSIEAAAGVTTGISVPD
                     RTHTIRVAANLNSKPEDLAQPGHIFPIMAQNGGVLVRSGHTEASCDLARLAGFSEMAV
                     VCEILKEDGTMARRHDLELFAKKHNLKIGTIADLIRYRLEREQMVVERNRAPLPTEWG
                     TFEVIHFENTQDKSKHLVLLFQTDKHSLPWIRIQSHDLLFDLPGMVQGHWDPNHLRWP
                     LSKAMQAIVEHGNGAIVLLNSETSSYNIVEKVGMLNESNNAQMQPPSSIPDWRDIGIG
                     SQIVSQLGYSRIAVLGSPKKLCGLSGFGLEVVEYLPYVTLASELYA"
     gene            complement(1195..>2366)
                     /gene="ribD"
                     /locus_tag="VFP93_00350"
     CDS             complement(1195..>2366)
                     /gene="ribD"
                     /locus_tag="VFP93_00350"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9843088.1"
                     /translation="KIFVLSMKKNMKQKIERDNYFMEQALLEAQQGLFTAHPNPRVGC
                     IFVKNENIIARGAHIATGYRHAEVQAIHHATQSLMDSTCYVTLEPCTHTGHTPPCVDT
                     LIAQKIQRCVIATVDPNPKVSGRGIQKLKASGIVVDVGIKQEEAQALNPGFFKRMLSN
                     KPFVFAKIASSLDGKTGMLTGESQWITSNASRLDAHYYRARVGAIITSWKTVNMDDPS
                     LTVRAPEILNQIPHPEFYKQPVRIILDTHLKTKPTAKIFDQAGTTWVMIRKNTTYEKI
                     RTWQTQLKNPANTKIVAIPCLQNRLNLQALLYLLAENQINEVLLECGSNLLGAFLQEK
                     LIDTCIFYFAPKILGSKTQGMFNLPLENLTSHIPLKITTVEKLQEDVKIIASCTE"
BASE COUNT          653 a          501 c          477 g          735 t
ORIGIN      
        1 aataggcttt ttcggtacgg tatagtttcc tttaatctca cgcatacagt tcactcgcta
       61 aggtaacata gggcaggtat tctaccactt ctaaaccaaa acccgatagg ccacataatt
      121 ttttgggcga gcctaaaact gcaattcgag aatatccgag ttgcgataca atttgcgagc
      181 caatgcctat atcacgccaa tctgggatag aggatggagg ctgcatttgt gcattattag
      241 attcatttaa catacccact ttctcaacta tattgtagct actcgtttcg ctatttaaaa
      301 gtacgatagc tccattgcca tgctcaacaa tagcttgcat ggctttagaa agtggccaac
      361 gaagatgatt gggatcccaa tggccttgta ccatgccagg taaatcgaac aacaaatcat
      421 gactttgaat acgtatccaa ggtagagagt gtttatccgt ttgaaacagt agaaccaaat
      481 gtttactctt atcttgggta ttttcaaaat ggattacttc aaaagtaccc cactcagttg
      541 gtaagggagc acggttgcgt tccacaacca tttgctctct ttctagtcga taacgaatca
      601 aatcggcaat ggtgccaatt tttaaattat gtttttttgc aaacaattct agatcatggc
      661 gtcgcgccat ggtgccgtct tccttcaaaa tttcgcacac tacggccatt tcagaaaaac
      721 ctgccaaacg tgctaaatca caactcgctt cggtgtgacc cgagcgcact aatacaccac
      781 cattttgtgc catgatggga aaaatgtgac caggctgcgc tagatcctca ggttttgagt
      841 taagattagc cgcaactcga atcgtatgcg tgcgatccgg caccgaaatg cctgtggtca
      901 cgcctgcggc tgcctcaata gataccgtaa actttgtacc aaatttcgat tgattgttga
      961 gtggcaccat taaaggcaaa tgcaaacgct cgcaatgttg tttgctcatg ggcaggcaaa
     1021 taagaccacg cgcaaattgc gccataaaat taatatgttc ttctttcact ttttcagcag
     1081 ctattacgag atcgccttca ttttctctgt cttcatcatc cataagaata acgagcttgc
     1141 cttgtttgat atcttcaatg agttctttag tagtatgcat aggtttctca atagttattc
     1201 ggtacaagaa gcaataatct ttacatcctc ttgtagcttt tccacggtcg taatttttaa
     1261 gggtatatgg ctggttaaat tctccaaagg taaattgaac ataccttggg tttttgagcc
     1321 taatatttta ggagcaaaat aaaaaatgca ggtgtctatc aatttttctt gtagaaatgc
     1381 accgagtaaa ttggatccac actccaacaa cacttcatta atctgatttt ccgctaacaa
     1441 atacaagaga gcttgcagat tcaagcggtt ttgtaaacag ggtatagcga caatttttgt
     1501 atttgcggga tttttcagtt gggtttgcca agttctaatc ttttcatagg ttgtgttttt
     1561 tcgaatcata acccaagttg ttcccgcttg atcaaatatt ttggcggttg gcttcgtttt
     1621 tagatgggta tctaaaataa ttctaacagg ttgtttataa aactctggat gaggaatttg
     1681 gtttaaaatt tctggggcac gcaccgttaa ggaagggtca tccatattga ctgttttcca
     1741 gctcgtaata attgctccca ctcgagcacg ataataatgc gcatctaatc gcgacgcgtt
     1801 ggaggtaatc cattgacttt cccccgtaag catgcccgtt ttaccatcaa gagaagaagc
     1861 gattttggcg aagacaaagg gtttattact caacatgcgc ttaaaaaagc cgggatttaa
     1921 tgcttgcgct tcttcttgct taattccaac atctacaaca atgcctgaag ccttgagctt
     1981 ttggatgcca cgaccggaaa cttttgggtt gggatccaca gtggcaataa cacacctttg
     2041 gattttttgt gcaattaacg tatccacaca aggtggtgta tgccctgtat gggtacaagg
     2101 ttctagcgta acatagcaag tgctatccat taaactttgg gttgcatgat ggatcgcttg
     2161 cacttcggca tgtctatagc ctgtcgcaat atgcgcaccg cgtgcgatga tgttctcatt
     2221 tttgacaaaa atacatccta cgcgcggatt agggtgagcc gtaaacaaac cttgctgcgc
     2281 ctcaagcaga gcttgttcca taaaataatt atcacgctca attttctgtt tcatgttttt
     2341 tttcatgctt agaacgaaga ttttgt
//
LOCUS       DASTIB010000022         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1235309, whole genome shotgun sequence.
ACCESSION   DASTIB010000022 DASTIB010000000
VERSION     DASTIB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235309"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFP93_00355"
     CDS             <1..875
                     /locus_tag="VFP93_00355"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET9843089.1"
                     /translation="ATFNSTEKYYIYGNSQGFLAGYPTTRHALSCVMIAQDAQGMQRD
                     YQYTIHRNPSLLKHAKIIGSEAAQRALDRLSSQRVPTQTIPVIFRADVATSLFSHFCS
                     AIAGSKLYRKASFLLDSLNTQLFPKNLEILEEPHLQQALGSAPFDADGVQTRSRYLIK
                     EGILQGYILSAYSARKLNMANTGNAGGIHNLIVSHQDITLKDLIHSQKKALFVTELMG
                     QGINLVTGNYSRGAAGFMIEDGEIKYPVHEITIAGNLKDMFKQMSAISNDVDRRGNIQ
                     TGSVLIESMMLAGS"
     gene            complement(1172..2563)
                     /locus_tag="VFP93_00360"
     CDS             complement(1172..2563)
                     /locus_tag="VFP93_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9843090.1"
                     /translation="MRFWLSLILIAFSFSAQAQKIGAQHQKSNVCADNYKKGNYALAF
                     TQCEQSAQKGNIHAQSTLGLMYFYGQGTVNDNDKAAKWFEQAALKNDAISQFHLGRMF
                     SLGLGVEINDAKAMHWFLAAAKQGIWEAQLMVSLSYRLGIGSPKDTNAAYMWYQSAIK
                     NGMPYAQEIKRENFQTRPFALNANLPGHPVYLQGMNLLMGIEGDPDPKNAAIWFEQAA
                     DLGHPDAQFNLAILYLEGDGIEKNEEKAYDWFLQASEQDHKEANAYLAFMNLLGIATA
                     YDPKVAIELYISSLEDNTTPIAQEENIKDPVLDAAEKGNPQAQYEIGLRFLHGDGVKK
                     DSIEAFEWLQEAATLNHNPAQYELGMMFLEGKGVAQDSKTAIHWLTKAAENNNPQAQY
                     ELGLLYFTGKFLQKNVQQAYAWFNVAASQDIVEAHAARSLVIQEMTAQEIEQAENLSR
                     PMHLNFNKTPFTP"
BASE COUNT          820 a          524 c          534 g          904 t
ORIGIN      
        1 gagctacctt taatagcact gaaaaatatt atatttatgg aaacagtcag ggttttttag
       61 cagggtatcc tacgacacgt cacgcacttt cctgcgtaat gattgcacag gatgcgcaag
      121 gtatgcaaag agattatcaa tatacgattc atcggaatcc aagcttatta aaacatgcta
      181 aaattattgg ttcggaagca gcccaaagag ctttagatcg attaagtagc caacgtgttc
      241 ctacccaaac catccctgtt atttttcgtg cggatgtggc aacttctcta tttagccatt
      301 tttgtagtgc cattgctgga tctaagctct accgaaaagc aagttttctt ttagattcgc
      361 ttaatacgca actatttcca aaaaatttag aaattttaga agaaccccat ttacagcagg
      421 cgttgggttc agcacccttt gacgccgatg gagttcaaac ccgatctcgc tatttaatca
      481 aagaaggtat tctccaaggg tatattttat ccgcgtattc tgcacgaaaa ttaaatatgg
      541 caaatacagg aaatgccggg ggtatacata atttaattgt atctcatcaa gatataacgt
      601 taaaagatct catccatagt caaaagaaag cattgtttgt aaccgagctt atgggacaag
      661 gcattaatct cgtgacagga aactattccc gaggggctgc cggttttatg attgaggatg
      721 gagaaattaa atacccggta catgaaatta ccatagcggg caatcttaaa gatatgttta
      781 aacaaatgag tgccatttcg aatgacgttg atcgccgagg aaatattcaa accggatcgg
      841 ttttgattga aagtatgatg ttggcaggga gttaaatttt tcgagacttt aggaatttga
      901 ttatgtgtaa atgtgctagg tgaatttcct tgattttttg atcgagaatt atatcatgta
      961 caccgcacgc gttttggagt atccctaact ttttattgcg aatatggagc aattcagatg
     1021 gggtgctatt tagatttgcg cctaatgttt catacgcatg taaaatttgt aaatcgttat
     1081 gcatttttta aaaaaaatag atataaacat taggaatagt aaatcacaat ctaaaggtat
     1141 aacccattga tctttagtac ctgtatataa tttaaggagt aaaaggtgtt ttattgaaat
     1201 ttaaatgcat tggtctggat aaattttcag cttgttctat ttcttgtgca gtcatttctt
     1261 ggattactaa acttcgtgcc gcgtgtgctt caacgatatc ttgagaagct gcaacattaa
     1321 accaggcgta agcttgttga acattttttt gtaaaaattt gccagtaaaa tacaaaagcc
     1381 caagttcata ttgggcttgc ggattattgt tttctgcagc tttggtcagc caatgaatcg
     1441 cagttttaga atcttgtgct acaccttttc cttctaaaaa catcattccc aactcatatt
     1501 gagcgggatt atgatttaag gtcgccgctt cttgaagcca ttcaaaagcc tcgatagaat
     1561 ctttttttac tccatcgccg tgtaaaaaac gtagccctat ttcatattgg gcctgaggat
     1621 tacctttttc agctgcatct aaaacaggat cctttatatt ttcttcttgg gctatcggtg
     1681 tggtgttgtc ttcgagcgaa gagatgtata gttcgatagc aacttttgga tcatacgctg
     1741 tggctatgcc taataaattc atgaatgcca gatacgcatt agcttcttta tgatcttgtt
     1801 ccgatgcctg caaaaaccaa tcataggctt tttcttcatt tttctcgata ccatcgcctt
     1861 ctaagtataa gattgctaaa ttgaattgtg cgtcaggatg acctaaatct gcggcctgct
     1921 caaaccagat ggcagcattc ttcggatccg gatcgccttc aattcccatc aaaagattca
     1981 tcccttgtaa ataaacgggg tggccaggta aatttgcgtt aagcgcaaaa ggacgggttt
     2041 gaaaattttc acgttttatt tcctgagcat aaggcatgcc atttttgatt gcactttgat
     2101 accacatgta agcagcatta gtatctttag gagatccaat gcccaaacgg tagcttaaac
     2161 tgaccatcag ctgcgcttcc catattccct gtttagcagc tgcaagaaac caatgcatcg
     2221 ctttcgcgtc atttatttct acccctaaac ctaggctaaa catacgacca agatgaaact
     2281 gagaaatagc atcgtttttt aaagctgcct gttcaaacca cttcgcagct ttgtcgttat
     2341 cgttaacggt accttgaccg taaaaataca ttaaacctaa agtagattgg gcgtgaatat
     2401 ttcctttttg cgcgctctgc tcgcattgcg tgaatgctaa tgcataatta ccttttttat
     2461 aattatcagc gcaaacattt gacttttgat gctgagcacc aattttttgc gcttgcgctg
     2521 aaaaagaaaa ggcaatcaaa atcagagaga gccaaaatct catgcatata ccttttttaa
     2581 gtactgcgac ccttgttagt ttagctttat attcttgagt ttaccaaata ggttagaagc
     2641 aaagactata tttaaagtat taaaaggggg ttattttgct acttttttat acggtcttcg
     2701 gattacttgt ttttagtatt gctatacatg gttttatggc aatgcactat tatcgaataa
     2761 aaaaaacgaa tcgcaggttg cg
//
LOCUS       DASTIB010000023         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_892250, whole genome shotgun sequence.
ACCESSION   DASTIB010000023 DASTIB010000000
VERSION     DASTIB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892250"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="VFP93_00365"
     CDS             <1..1597
                     /locus_tag="VFP93_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843091.1"
                     /translation="KLITYGLSGLGIALGSLGNSWSIWGGILLGTGAYLGTKLSLLTK
                     LQKKTLFEQNVASLQPTRTNHPIFKTKRETFNQIFSFILDHENNIWYALRDPHVTPNW
                     KPIYIEDFPPPDVKEVELKSDGENLIAKATLYASKENKSTLFYKKVYQEHRDDDVYSI
                     YNVADSPYSINQWFTLPLVSWLTPQYWNKRLTIQSQSRWAVSHSGKLRLHIQDKKLQW
                     HAIWGVTTIIEQRHQDLILYDPYWPPGFEIKISLPPEFMLIDLATTSSKVFLLGRSYK
                     GEWMLCTSSLDYDILGLNPFMHYTLDASQKNARLLPLTNWETTKISLQNLTGKINAIQ
                     FGVDKNTQMQLEWCDTTTRGFYFKNLIEDAWYSHKLCDLEKEDWYTLDSLPQQPLILK
                     NTVFFGKKFSELAIAHFDEHCLNAKITLKNAQEIIIFNLQRTSNIVNDFLTGKPNYYW
                     ILSLAPDFQTEENRKWLKNSLNFPVQIEHTLNYTLINCPDKFSLRFEPVKGKMLCFSD
                     RSLKNSENQDEKLEAFFKQKVSL"
     gene            1600..>2097
                     /locus_tag="VFP93_00370"
     CDS             1600..>2097
                     /locus_tag="VFP93_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9843092.1"
                     /translation="MHVIHKVAMDQAIHNLLNFYKNKKFCKETIHTVLTNLAFSQSVT
                     YPKPPITGAAFKGGGVKVLAHIGAILAHDIYGHKGIDRLKYVAGSSGGGIVAFLIAMG
                     FSALQIFEFVNHIKLTDFLDTGQKNNTQAFPLSNVVDFIKSGSIHSGENFRNWALSII
                     EFVLGS"
BASE COUNT          713 a          342 c          363 g          679 t
ORIGIN      
        1 aaaacttata acttatggtt tatcagggct tggaattgca ttaggatctt tgggcaattc
       61 ctggagcata tggggtggta ttctcttggg gacaggagcc tatttgggta ctaaattatc
      121 tttattaaca aaactacaaa aaaaaacttt atttgaacag aacgtagctt cattgcaacc
      181 cacgcgcacg aatcatccta tttttaaaac aaaaagagaa acattcaatc agatatttag
      241 ttttatacta gatcatgaaa ataatatttg gtatgcccta cgcgatcctc atgtcactcc
      301 aaattggaaa ccgatttata tagaagattt tcccccgcct gatgttaaag aagttgaatt
      361 aaaatccgat ggtgaaaatt taatagcaaa agcaaccctt tatgcttcta aagaaaataa
      421 atcaacatta ttttataaaa aagtatatca agaacataga gatgacgatg tttatagcat
      481 ttataatgtg gctgacagcc cttattctat taaccaatgg tttactttac ctctagtttc
      541 atggttaaca ccacagtatt ggaataaaag attaacaatt caaagccaat cgcgttgggc
      601 ggtatcacat tcaggtaagc tacgattgca cattcaagat aaaaagttac aatggcatgc
      661 catttggggg gtgacaacta tcatagaaca aagacatcag gatttaattc tttacgatcc
      721 ttattggcct ccaggttttg aaatcaaaat atccctacca cctgagttta tgcttatcga
      781 cctggcgaca acaagctcta aagtattttt gttagggcga tcttataaag gggagtggat
      841 gttgtgtacc tcttctttag attatgatat tttgggactt aatcccttta tgcattatac
      901 cttggatgca tcacaaaaga atgcaaggtt attaccttta acaaattggg aaaccacaaa
      961 aatttctttg caaaatttga caggaaagat aaatgctatt caatttggtg tagacaaaaa
     1021 tacgcaaatg caactcgagt ggtgtgatac gactacgcgt ggattttatt ttaaaaattt
     1081 gatagaggat gcatggtata gtcataagtt atgtgattta gaaaaagaag attggtatac
     1141 tcttgattct ttgccccagc aacctttaat tttaaaaaat acagtatttt ttgggaaaaa
     1201 atttagtgag ctcgcaattg cgcattttga tgaacattgc ttaaacgcaa aaatcacact
     1261 aaaaaacgcg caagaaatta ttatttttaa tttgcaacgc acttccaata tcgttaatga
     1321 tttcttaaca ggcaagccaa attattattg gatattatct ctcgctccag attttcaaac
     1381 agaagaaaac agaaaatggt taaaaaactc cttaaatttt cctgttcaaa tagaacacac
     1441 cttaaattat acccttataa attgtcctga caaattttct ttacgctttg aacctgttaa
     1501 aggtaagatg ttgtgttttt ccgatcgttc tcttaaaaac agtgagaatc aggatgaaaa
     1561 attagaggct ttttttaaac aaaaagtaag tttataaata tgcatgttat tcacaaagtt
     1621 gcaatggatc aagctatcca taatcttctt aatttttaca aaaataaaaa attttgtaaa
     1681 gaaacaattc ataccgttct aacgaattta gctttttcac aaagtgttac atatcctaag
     1741 ccgcccatta ctggagctgc ctttaaagga ggaggggtaa aagttttagc acacattgga
     1801 gcaatactgg cacatgatat ttacggtcat aagggcattg atcgattaaa atacgttgca
     1861 ggttcttcag gcgggggaat tgtagctttt ttaattgcca tgggcttttc tgcgctccag
     1921 atctttgagt ttgttaatca tataaaacta acagatttcc tggatactgg acaaaaaaat
     1981 aacacgcaag cttttcctct gagtaatgta gtcgatttta taaaatctgg ttctattcat
     2041 tctggtgaaa atttcagaaa ttgggcttta tctattatag aatttgtttt ggggagt
//
LOCUS       DASTIB010000024         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_57688, whole genome shotgun sequence.
ACCESSION   DASTIB010000024 DASTIB010000000
VERSION     DASTIB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57688"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VFP93_00375"
     CDS             complement(<1..821)
                     /locus_tag="VFP93_00375"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9843093.1"
                     /translation="MQTQGLLKEGKKSESYPRNIAPDFSLQSLNPIIQSLNKEINITA
                     VLCYGSYAEGTQDEKSDIDLLVICDDLIPESSVRYKIYEKNNGQQIKLQKPHENWETT
                     WTPINDEFLYKGKKIEIGYNLSSWVKDVVNKIVNEGKTTLEDFQFRPYTFLGLLENSV
                     CLFEKENFISLIRKQIRPFPPTLKAEIVKENFSIFNESLEDLEDFNHRDIGLLAFQFM
                     LFRGLDAAIQIIFAINEVYYPASKREEKHLMRLPKLPQGMHELIYDLLPGFFNRK"
     gene            complement(804..1238)
                     /locus_tag="VFP93_00380"
                     /pseudo
     CDS             complement(804..1238)
                     /locus_tag="VFP93_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1279..2370)
                     /locus_tag="VFP93_00385"
     CDS             complement(1279..2370)
                     /locus_tag="VFP93_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HET9843094.1"
                     /translation="MEKLKKTKNKNHISIGKQSFPEFNKDSVYLEFLADIKKRYQIAQ
                     LKAAQVVNQELIQFYWHLGKQIVEKQAQAIWGSKFLEQLSRDLQIGFPGSSGMSVRNL
                     KFMRQFAQLYPDEIGKQPVSQLPWGHIIVLMQRIKDPEIRHWYASNTIQNGISRSVLL
                     MQIETGLYERQGRLDHKTSNYLKKLPPPQSDMAHEMLKDPYKLDFLSVRDEAHEKELE
                     HALIHHIRDFLIELGQGFAFVGSQVPVTINSKNYFIDMLFYHLKLRCYIVVELKVVDF
                     EPEFAGKLGFYLAAIDNILRHPADHKTIGLLLCKSKDKIVAEYALQNIDAPIGVSEFI
                     LSKALPKELKDNLPPIEELEAELSASENE"
     gene            complement(2439..>2651)
                     /locus_tag="VFP93_00390"
     CDS             complement(2439..>2651)
                     /locus_tag="VFP93_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9843095.1"
                     /translation="YGLSEENRVGLVDLMLERLQALFSFLMQSAREGNKKYAANVQNG
                     HHLTYLADIEYITLHKLRIENGLIRV"
BASE COUNT          803 a          527 c          397 g          924 t
ORIGIN      
        1 ttttttctgt taaaaaaccc tggcagaaga tcgtaaatca gctcatgcat gccttgcggt
       61 aattttggca aacgcattaa atgtttttct tctctctttg aggcaggata atacacttca
      121 ttaatagcaa atattatttg aatagctgcg tctaatcctc taaacagcat gaattgaaaa
      181 gcgagcaatc caatatcacg atggttaaaa tcttcgagat cttctaagct ttcattaaag
      241 atagagaaat tttcttttac aatttctgct ttcaatgttg gtggaaaagg acggatctgc
      301 ttccttatca gcgatataaa attttctttt tcaaacaagc acactgaatt ttccaataac
      361 cccaaaaatg tgtagggtct aaattgaaaa tcctctaacg ttgttttacc ttcattaaca
      421 attttgttga ctacatcttt tacccatgac gatagattat agcctatttc aattttctta
      481 cctttgtaaa gaaattcatc attaatgggt gtccaagttg tttcccaatt ttcatgcggt
      541 ttctgcagtt ttatctgctg accattattt ttttcatata ttttatagcg aacactagac
      601 tctggaataa gatcatcaca aatgactagt aaatcaatat ctgatttttc atcttgcgtc
      661 ccttcagcat aagagccata acaaagcacc gctgtgatgt taatctcttt atttaaagat
      721 tgaatgattg gattcagcga ctgcaatgaa aaatctgggg cgatatttct tggatagctc
      781 tcacttttct tgccttcttt taaaagacct tgcgtttgca cctcgtcatc gaaaaatttg
      841 acaaaaaatg tcttagtcaa ttttggattt ttagtattct ctctcacaaa ttctgcttta
      901 aatccacatt ttttataaaa ttctggagct tggaagctac ttgtcactag attgatatta
      961 ttgaatccct ttcctttaaa atgattttca agctctgaaa tcaactttct tccataaccc
     1021 ttaccacgat gggatttatc tacccatata tcatcaatat aaatttcagc aaatgcggta
     1081 aatgcattta aaacaccaat ggcttctttt ttttcatcct tgagaaccaa tgaaaacggt
     1141 ttatagttaa catcaatccc gtgactactt tcatactcca cgaagccttt tcgcattttt
     1201 tcttcaacat catcgggaat cttttcgaca aactcaactt tgcaattttt catatgttgc
     1261 tcgtttaaca tgaaaccatt attcattttc agaagcacta agttctgctt caagctcttc
     1321 aatcggtggc aaattgtctt tcaattcctt tggtaacgct ttagaaagaa tgaactcaga
     1381 tacgccaata ggtgcatcta tattttgaag cgcatattca gcgacaattt tatcttttga
     1441 tttgcatagc aaaagcccaa ttgttttgtg atctgctgga tggcgcagaa tgttatcaat
     1501 tgcagcaagg taaaacccaa gcttacctgc aaattcaggt tcaaaatcaa ccactttaag
     1561 ttcgactaca atatagcaac gaagcttgag atgataaaac aacatatcaa taaaataatt
     1621 tttactatta atggtaactg gtacttgaga acctacaaaa gcaaagcctt gccctaactc
     1681 aataagaaaa tcgcgtatgt gatgaattaa cgcatgttct agttcttttt catgggcttc
     1741 atctcttaca gataaaaaat caagtttata cgggtctttc aacatttcgt gagccatatc
     1801 agactgggga ggaggaagct tctttagata attgcttgtt ttatgatcta atcttccttg
     1861 tctctcataa agacccgttt caatttgcat caagagaaca ctgcgcgaaa taccattttg
     1921 gatggtattg ctggcgtacc aatgccttat ttctggatct ttaattcgtt gcatcaatac
     1981 gatgatatgc ccccatggca attgggaaac aggctgtttc ccaatttcat ctgggtataa
     2041 ttgggcaaat tgacgcataa attttagatt tctaactgac attcctgaag agccagggaa
     2101 cccaatttgc aaatccctag ataattgttc taaaaattta ctcccccaaa ttgcttgagc
     2161 ttgcttttcg actatctgct tgcccagatg ccaatagaac tgaataagtt cctgattaac
     2221 cacctgcgct gctttaagct gcgcaatttg ataacgtttc ttaatatcgg ctaaaaattc
     2281 tagataaact gaatccttgt taaattcagg gaacgattgt ttcccaatgc taatatggtt
     2341 tttatttttt gtttttttta atttttccat ttaaataacc tttaaatcaa ttttagcatc
     2401 ataataaatg gcactcactg ctaggcaagt agcatcattt aaacccttat taagccattc
     2461 tcaatccgta atttatgtaa cgttatatat tcaatatctg ctaaataagt taagtggtga
     2521 ccattctgca cattcgctgc atattttttg tttccctctc gagctgactg catcaaaaag
     2581 ctaaacaagg cttgtaacct ttcaagcatt aaatcaacta atccaactct attttcttca
     2641 ctcaatccat a
//
LOCUS       DASTIB010000025         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_72667, whole genome shotgun sequence.
ACCESSION   DASTIB010000025 DASTIB010000000
VERSION     DASTIB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72667"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /gene="topA"
                     /locus_tag="VFP93_00395"
     CDS             complement(<1..2057)
                     /gene="topA"
                     /locus_tag="VFP93_00395"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9843096.1"
                     /translation="MSNRLVIVESPAKAKTLKKFLGSGFQVVASYGHVRDLIPQEGAV
                     DPDNDFSMHYEVIAKNAKHVKTIADHLKNSTELLLATDPDREGEAIAFHVSELMKQKK
                     LLKEIPVRRVVFHEITKSAILDAIENPRDLSLDLVHAQQARRALDFLVGFNLSPLLWR
                     KVRPGLSAGRVQSPALRLIVEREEEIERFQAQEYWTIESLLEKEAQEFAARLVEFKKE
                     KLKQFSICDEATAKKMEKDLLKAAKQGLKVVKVEKKQRKRNPAAPFTTSTLQQEASRK
                     LGFGAARTMRIAQQLYEGIDIGQGSVGLITYMRTDSVVLAMTAIEEIRDVIQKRFGNE
                     NLPSEPRLYKTKAKNAQEAHEAIRPTSAQVSPTQIKEYLTPEQLKLYTLIWKRTLACQ
                     MIHATIDTVAADLECGPGNIFRANGSTIAFPGFLQVYEEGLDDVSKESEKILPPLEEG
                     EILPLKEMKTTQHFTEPPPRYSEATIVKTLEEYGIGRPSTYASIISTLQQRDYAQLIT
                     KRFHPTDVGRVVNRFLTTYFTQYVDYGFTAGLEDELDAVSRGEQPWKPLLEHFWSPFI
                     DKVKEIEKNVQRKDVTQEMIDEKCPECGSQLSIRLGKRGRFIGCTGYPDCQYTRSLDE
                     TREEANATEIIPDRKCPECNNDLIIKKGRYGKFIGCSNYPACKHIEPLEKPQDTGVQC
                     PKC"
     gene            complement(2090..2566)
                     /locus_tag="VFP93_00400"
     CDS             complement(2090..2566)
                     /locus_tag="VFP93_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HET9843097.1"
                     /translation="MKETELDVLMFLFDNYLGVEVEFSGGENAIADELKQAGFHEQDI
                     NKAFDWLSTLSDLYQDGYELPEHSRALRVLSTQECAKLDVKAQGYLWQLEGQGVLDPT
                     TREIILESAMAIDVQTLNLQQFKRIVALVLLNNPKAEAIVASGEDLVYQEIGGIFH"
     gene            complement(2580..3692)
                     /gene="dprA"
                     /locus_tag="VFP93_00405"
     CDS             complement(2580..3692)
                     /gene="dprA"
                     /locus_tag="VFP93_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9843098.1"
                     /translation="MASATLEQEKSYWLLLNRIPGFGPAKFHYLLKQETCLANWFIGY
                     SPNADLVNLFDTWNITPMLDWQGVEQDLNWELSHNCYIILRHDALYPPLLKEIANPPH
                     VLFLQGNIATLSFHQIAMVGSRNPSQQGCENAFLFAKRLAQEGLTITSGLAIGIDTWS
                     HKGALACNGFTIAVLAHGLDTIYPASNKKLVDQILQNKGALLSEFAIGTLPRHSHFPR
                     RNRIISGLSLGVLVVEATLKSGSLITAKFAVEQGREVFALPGSIHNPLAKGCHHLIRQ
                     GAKCIESQEHILEELRPQQTPCISEPARIKSTDTNKENLPSDLHKILNHIGFECTPLD
                     RIVEQSGLPSSKVSASLVELELHGAITLESGGYVRV"
     gene            complement(3728..>4568)
                     /locus_tag="VFP93_00410"
     CDS             complement(3728..>4568)
                     /locus_tag="VFP93_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843099.1"
                     /translation="LHVDRSQNLRAQPVTVIEETHETIVEIRPRVRYLPPTKAIPTIP
                     YNVIGPFLSRSQVMPEGILDSATAIVAVDEDHLLVGEGNRFFTPLITVPVGGQVSLFR
                     SGSLLTHPDTEEILGQVAFYLGSAEVESHSMTTSLIVQKSREEMRPGDRLCPIEEEII
                     EPYFFPKMPQGFAYGYILSVFGGVNQIGQNQVVVISGGYDSMREIGDVLAIYQTKEDL
                     PPRLRKKENFYAYMCGETTDLQFAPILVGRLIIFRVFDKTSLGLIVSATRPIYLQDVV
                     MSP"
BASE COUNT         1275 a          993 c          882 g         1418 t
ORIGIN      
        1 tgacatttgg gacactgcac tccagtatct tgcggctttt ctaatggttc aatatgttta
       61 cacgcaggat aattactaca gccaataaat tttccgtatc gacctttttt aatgatcaaa
      121 tcattattac attcaggaca ttttcgatcg ggaataattt ctgtggcatt tgcttcttca
      181 cgcgtttcat caagtgatcg ggtatattgg caatcgggat accctgtaca tcctataaag
      241 cggccacgtt ttcctaaccg aatcgaaagt tgactcccac actcaggaca tttttcatcg
      301 atcatttctt gtgtcacgtc tttgcgttgc acattttttt ctatttcttt taccttatcg
      361 atgaagggag accaaaaatg ttctaacaaa ggtttccaag gttgttcacc acgagaaact
      421 gcgtctaatt catcttccaa gcctgcagta aagccatagt ctacatattg cgtaaaatag
      481 gtggttaaaa acctattcac caccctacct acatccgtag gatggaagcg tttagtaatg
      541 agttgggcat aatcacgttg ttgcaaagtt gaaataatag atgcatacgt ggaaggtcta
      601 ccaattccat attcttctaa tgtctttacg atggttgcct ctgagtaacg tggcggaggt
      661 tctgtaaagt gctgggttgt tttcatttct tttaacggta aaatttctcc ctcttctaat
      721 ggaggtagaa ttttttcgga ttcttttgaa acatcatcca aaccctcttc ataaacttgt
      781 aaaaatccgg gaaaagcaat ggtcgaacca ttggcacgaa aaatattacc cggcccacat
      841 tccagatcgg cagctacggt atcaatggtt gcatggatca tttgacaagc caaagtacgc
      901 ttccatatca gcgtatacaa ctttagttgt tcgggtgtta aatattcttt aatttgcgtt
      961 gggcttactt gcgccgaggt ggggcgaatg gcttcatgtg cttcttgcgc attttttgct
     1021 tttgttttat ataatctcgg ctcagaaggt aaattttcat taccgaagcg tttttgaatg
     1081 acatcgcgaa tttcttctat cgcagtcata gcaagaacca ccgaatcggt acgcatataa
     1141 gtaatcaaac ctaccgatcc ttgaccaatg tcaatacctt catacaattg ttgcgcaatg
     1201 cgcatcgtgc gagctgcacc aaagccaagt ttacgagaag cttcttgttg taaagtagaa
     1261 gtagtaaaag gagcggcagg gtttcttttg cgctgttttt tttcaacttt aacaacctta
     1321 agcccttgct ttgctgcttt tagtaagtct ttttccattt tcttagcggt agcttcgtcg
     1381 catatcgaaa actgtttaag tttttctttt ttgaattcta caaggcgtgc cgcaaactct
     1441 tgcgcttctt tttcaagcag tgactcgatt gtccaatatt cttgcgcttg aaagcgctca
     1501 atttcttctt cacgttccac gattaatcga agcgctggac tttgcacacg acccgcggat
     1561 aatcctgggc gcactttacg ccacaataag ggagataaat taaagccaac taaaaaatcg
     1621 agcgctctgc gcgcttgttg ggcatgaacc aaatccaggg aaagatcacg agggttttca
     1681 atggcgtcta aaattgcact ttttgtaatc tcatggaata caacgcggcg aacgggtatt
     1741 tcttttaata atttcttttg cttcatgagc tcgctgacat gaaaagcaat tgcttcccct
     1801 tcgcgatccg ggtcggttgc tagcaataat tcggtactat tttttaaatg atcagcaatc
     1861 gtttttacat gcttggcatt ttttgctatt acctcatagt gcatggaaaa atcattgtcg
     1921 ggatccacag caccttcttg aggaattaaa tcacgaacat gaccatacga ggccacgact
     1981 tgaaacccgc tgcctaagaa tttttttaga gtctttgctt ttgccggcga ctcgactatc
     2041 acgaggcgat tgctcatgca cttccaacca tgtaaactaa ttaactaaac taatgaaaga
     2101 ttccgccaat ctcttgatag acgagatcct caccgcttgc cacgatagcc tcggccttag
     2161 gattgtttaa tagcactaaa gcaacgatgc gtttaaactg ctgcaaattg agggtttgta
     2221 catcaattgc catagcactt tcaagaatta tttcgcgcgt ggtgggatct aacactccct
     2281 gaccttcaag ttgccacaaa taaccctgcg ctttgacatc aagttttgca cattcctgtg
     2341 tgcttaagac tcgcaatgct ctcgaatgtt cgggtagctc ataaccatct tgatacaaat
     2401 cagacaaagt actcaaccag tcaaatgcct tgttaatatc ctgctcatga aagccagctt
     2461 gctttaactc gtctgcaatt gcattttcac caccagaaaa ttcaacttca acaccgaggt
     2521 agttgtcgaa taaaaacatt aaaacatcga gttctgtttc tttcataacg agacacctct
     2581 tatacacgta cataccctcc agactctaag gttatcgctc catgaagttc taattctact
     2641 aaggaagctg aaacttttga gcttggtagt ccgctttgtt caacaatcct atctaaagga
     2701 gtacattcaa agccaatatg atttagaatt ttatgtagat ccgaaggtaa attctccttg
     2761 tttgtgtcag ttgattttat gcgagcaggc tcacttatac atggagtctg ttgtggtcgt
     2821 aattcttcca aaatatgttc ttggctttca atacattttg cgccttgacg tatcaaatgg
     2881 tggcaacctt tcgctaatgg gttatgaata gatccaggta atgcaaaaac ttcacgtcct
     2941 tgttcaacag caaatttagc ggtaatcaat gaaccacttt tcaatgtagc ctcaacaacc
     3001 aacaccccta agcttaatcc gctaatgatt cgatttcggc gaggaaaatg tgaatggcgc
     3061 ggcaaggttc caatagcaaa ttcagaaagc agagctccct tgttttgcaa aatttgatca
     3121 acaagttttt tgttggatgc aggataaata gtgtctaagc catgggccaa caccgcaatt
     3181 gtaaaaccgt tgcaggctaa agctccttta tggctccacg tgtcaatacc tatagctaag
     3241 ccggaggtga tggttagtcc ttcttgtgcc aaacgtttgg caaataaaaa agcattctca
     3301 cacccttgtt gacttggatt tctgctgcca accatagcga tttggtgaaa acttaaagtt
     3361 gcaatatttc cttgtaggaa taacacgtgg ggtggattag caatttcttt caacagtggc
     3421 gggtatagtg catcatggcg aagaataatg tagcaattat gtgaaagttc ccagtttaaa
     3481 tcttgctcaa ctccctgcca atccaacatt ggggtaatgt tccatgtgtc gaacaaattt
     3541 accaaatctg cgttaggcga ataacctata aaccaattgg caagacaagt ttcctgttta
     3601 agtaaatagt gaaattttgc aggcccaaac ccaggaattc tattcaatag gagccagtag
     3661 gatttttctt gttctaaggt agcactggcc attcactttc tccttggccg gatttaaatg
     3721 cgcaagttta tgggctcatg accacatctt gcaaataaat tggtcgggta gcgctaacta
     3781 ttaatccaag gctcgttttg tcaaatactc taaaaataat tagtcttcct actaaaatag
     3841 gtgcaaactg caaatccgtg gtttctccgc acatatatgc ataaaaattt tcttttttcc
     3901 tcaatcgcgg tggcaaatct tctttggttt gataaatagc aagaacgtcg ccaatctcac
     3961 gcatggaatc gtacccgccc gatataacca caacttgatt ttgtccaatt tggttaacgc
     4021 ccccaaatac acttaaaata taaccataag caaaaccttg cggcatttta ggaaagaagt
     4081 aaggttcaat aatttcttct tcaatcggac aaagtctatc acctggacgc atttcttcgc
     4141 ggcttttttg cacaattaag gaagtggtca tactatggct ttctacttcc gctgagccta
     4201 agtaaaatgc tacttgtccc aaaatttctt cagtgtcagg atgtgttaaa agcgaaccac
     4261 ttcgaaataa acttacttga cctcctacgg gaacagtaat caagggggta aaaaatcgat
     4321 tcccctcgcc cactaataaa tgatcttcat ctaccgctac aatagccgtg gcagaatcta
     4381 aaattccttc tggcattact tgtgagcgcg ataggaaggg gcctatcaca ttgtatggaa
     4441 tagtgggaat agccttggtt ggaggtaaat atcgtacgcg tggcctgatt tctacaattg
     4501 tttcgtgtgt ttcttcaatt acggtaaccg gttgtgctcg taaattttgc gatctatcaa
     4561 catgtagt
//
LOCUS       DASTIB010000026         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_757882, whole genome shotgun sequence.
ACCESSION   DASTIB010000026 DASTIB010000000
VERSION     DASTIB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757882"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /gene="atpD"
                     /locus_tag="VFP93_00415"
     CDS             <1..815
                     /gene="atpD"
                     /locus_tag="VFP93_00415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9843100.1"
                     /translation="YHEMKESNVLDKVSLVYGQMNEPPGNRLRVALTGLTLAEQFRDE
                     GRDVLLFIDNIYRYTLAGVEVSALLGRMPSAVGYQPTLAEEMGRLQERITSTKKGSIT
                     SIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVDPLDSTSRQLDPLIV
                     GQEHYEVARAVQNTLQRYKELKDIIAILGMDELSEDDKLVVARARKIQRFLSQPFFVA
                     EVFTGSPGKYVSLKDTIQGFKGIIEGTYDDLPEQAFYMVGSIEEAVERAKTL"
     gene            836..1252
                     /locus_tag="VFP93_00420"
     CDS             836..1252
                     /locus_tag="VFP93_00420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET9843101.1"
                     /translation="MTLHLDIVSAEAEIYSGRAERVFASGTLGELEVAPGHAPLLTSL
                     LPGPLRIREQGGKEHVIYVTGGVLEVQPQIATILADTVVRAKDFNEAEALKAKQEAEE
                     ALKDKKASMDYAKARAELIRAAGMLKAIEKMKKRKQ"
     gene            1295..2686
                     /gene="glmU"
                     /locus_tag="VFP93_00425"
     CDS             1295..2686
                     /gene="glmU"
                     /locus_tag="VFP93_00425"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET9843102.1"
                     /translation="MSLSVVILAAGKGKRMMSTLPKVMHPVGGKAMLHHVVDLAMHLS
                     AEQVIVVHGEDKEPLRHALSNPSILWALQKNQLGTGDAVSQAMPFIPSKNYVLILYGD
                     VPLLTGASLTRLLQGTPINAMGLITAHVDNPFGLGRIIRNNENQVLGIREEKDANDQE
                     RQIHEINTGIFLLPAHYLEKWLPRLQNKNAQQEFYLTDIVEQAVAEGVKIFTTHPVHV
                     EEVLGINDKIQLAQAERCLQKRHADHFLKAGVRIFDPARFDVRGALHTGSDVSVDIGV
                     IFEGEVSLGNNTHIGPYCILKNCQLEENVTILAHCHLEGVKIKKGAVIGPFARIRPGT
                     EIGESAKVGNFVEIKNTIVGNESKISHLSYIGDATIGTSVNIGAGTITCNYDGTHKHQ
                     TYIEDYAFIGSGTQLVAPVKVESGAMVGAGSTLTKDAPRHQLTLTHKLEHRTRPWQKP
                     PNKLQALQDLEEN"
     gene            2695..4527
                     /gene="glmS"
                     /locus_tag="VFP93_00430"
     CDS             2695..4527
                     /gene="glmS"
                     /locus_tag="VFP93_00430"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9843103.1"
                     /translation="MCGIISGVAEREIAPLLLEGLKRLEYRGYDSAGMATITDAGALE
                     RLRICGKVQTLKEALDKSPLKGHLGIAHTRWATHGSPSETNAHPHMSSEKIAVVHNGI
                     IENYQELKENLIKEGFVFSSETDTEVIAHLLYRNLQHTNHNLVRAFYLTLPQLKGAYS
                     IAVLVKGEKNRILAARHGSPLVIGIGIGEHFVASDIIALLPVTQTFVVLEDGDVAEIF
                     KDHFQVYDTTAQPVHRPLKQVKISLQHAEKGEFRHFMQKEIFEQPRALSDTLSGRIQA
                     HQINASIFGTRASDVFPKVEHVHIVACGTSFHAGEVAKHWIQNLLGVPCSVEIASEMR
                     YQRQVVLPNTLFLCLSQSGETADTIAALRSTKENNNYLARLCICNVAESSLAREADIT
                     FLTHAGPEIGVASTKAFITQLIALLLFTFSLGKEKSYQPKAELLKTLANVPDLIEVTL
                     QLDEKIRELSKLFADKKNALFLGRGALYPIAKEGALKLKEISYIHAESYPAGELKHGA
                     LALVDKDMPVVALACNDHLLDKLRSNLEEVRARHGHLIVFATEDTKLTLDAPGIVVEL
                     PSVPTELAPVVMTIPMQLLAYHVAVLKGTDVDQPRNLAKSVTVE"
BASE COUNT         1355 a          879 c         1038 g         1271 t
ORIGIN      
        1 tttaccatga aatgaaagag tccaacgtat tggataaagt ttctctcgtg tacggacaaa
       61 tgaatgaacc acctggaaat cgtttacggg tagcgcttac cgggttaacg cttgcagaac
      121 aatttcgaga tgaaggccga gacgttttgc tgttcatcga taatatttat cgttatacct
      181 tagccggggt agaagtttct gctttgttag gtcgcatgcc atcagcagta ggttatcaac
      241 cgactttggc ggaagaaatg gggcgcttgc aagagcgtat tacttcaacg aaaaaaggat
      301 cgatcacttc catacaagct gtgtatgttc cggcagacga tttaaccgat ccatctcctg
      361 caactacttt tgcgcacttg gatgcaaccg tggtgttatc cagacaaatt gctgaacttg
      421 gtatttatcc tgcagtagat cctttggatt ccacaagccg ccaattagat cctttaattg
      481 tgggacaaga gcattatgaa gtagcgcgtg ccgtacaaaa cactttgcag cgctataaag
      541 aattaaagga cattattgcc attttaggta tggatgagct ctctgaagat gacaagcttg
      601 ttgtggcacg cgctagaaaa attcaacgtt ttctttcgca gcctttcttt gtggcggaag
      661 tttttaccgg ttcgcctgga aagtatgttt ctcttaagga cactattcag ggtttcaaag
      721 gcattattga aggaacgtat gatgacttac ctgaacaagc tttttacatg gttggctcca
      781 ttgaagaagc ggtagaaaga gcaaaaacac tttaactgga tctggaatta tggccatgac
      841 attgcatttg gatattgtca gcgcagaggc cgaaatttat tcgggtcgcg ccgagcgcgt
      901 ttttgcgtca ggaaccttag gtgagctgga agtggccccc ggccatgcgc cacttttaac
      961 gtcattgttg ccaggtccgt tgagaatacg agaacaaggt ggcaaagaac atgttattta
     1021 tgttaccggt ggggttttag aggtccaacc acaaattgcc accattttgg cagacacggt
     1081 agttcgcgca aaagacttca atgaagcaga agcgctaaaa gcaaaacaag aagcggaaga
     1141 agcgttgaaa gataaaaaag cttctatgga ttatgccaaa gcacgagcgg aattaattcg
     1201 tgcggctggc atgttaaaag ccattgaaaa aatgaaaaaa aggaaacaat aacacaagga
     1261 taagttcgaa tagcgcttga ataggaagca tagtatgagt ttgtctgttg taattttagc
     1321 tgcaggcaag ggtaagcgaa tgatgtctac tctgcccaaa gtaatgcatc ctgttggtgg
     1381 caaagccatg ttacatcatg tggtagattt ggcaatgcat ttatctgcag aacaagtgat
     1441 tgttgtgcac ggtgaagaca aagagcctct gcgacatgcc ctgtccaacc cttctattct
     1501 ttgggctctt caaaaaaatc aactaggtac tggtgatgcg gtttcacaag cgatgccttt
     1561 tatcccaagc aaaaattatg tactcatttt atatggcgat gtaccgttac tgactggagc
     1621 atctttaacc cggctccttc agggaacacc catcaatgca atgggattga taacagcgca
     1681 cgtagataat ccttttggcc ttggacgtat tatccgaaat aatgaaaatc aagtgcttgg
     1741 tattcgtgaa gaaaaagatg cgaatgatca agagcgccaa atccacgaaa tcaataccgg
     1801 aatattttta ctccctgccc attatttaga aaaatggttg ccgcgactgc aaaataaaaa
     1861 tgcgcaacaa gaattttatt taacagatat cgtcgaacaa gctgttgcag agggtgtaaa
     1921 aatttttacc acgcatcctg tgcatgttga agaggtcttg ggcattaacg ataaaatcca
     1981 acttgctcag gctgagcgat gcttacaaaa acgtcatgcc gatcatttct taaaagcggg
     2041 tgttagaatt ttcgatcctg ctagatttga tgtgcgtggt gctttgcata cgggctctga
     2101 tgttagtgtt gatatcggcg tgatttttga aggtgaggtt tccttaggaa ataacacaca
     2161 tattggtcct tattgcatcc ttaaaaattg tcaacttgaa gagaatgtaa ccatcttggc
     2221 gcattgccat ctagagggag taaaaataaa aaaaggcgct gttattggac cttttgctcg
     2281 cattagacca ggtacagaaa ttggcgaatc cgccaaagtg ggtaactttg tagaaatcaa
     2341 aaatacgatt gtgggcaatg aatctaaaat tagtcattta agttatattg gcgatgctac
     2401 tataggtact tcggtgaata taggcgcagg aaccattact tgcaattacg atggaaccca
     2461 caaacatcaa acttatatag aagattatgc ttttattggt tctggaacgc aattagtagc
     2521 gcctgtgaaa gttgaaagtg gtgctatggt aggggcaggc tcgactttga ctaaagatgc
     2581 accaagacat caactaacac ttacccataa gcttgaacat cgaacacgtc catggcaaaa
     2641 gccgccaaat aaattgcaag ctcttcaaga tcttgaagag aactaggagg attcatgtgt
     2701 ggaattatca gcggcgttgc agaacgtgaa atcgcacctt tgctcttgga aggacttaaa
     2761 cgtttagaat acagaggtta tgactctgca ggtatggcca ccattacgga tgcaggcgca
     2821 ctagagcgtc tgagaatttg tggcaaagtg caaaccttga aagaagcttt ggataaaagt
     2881 cctctaaaag gacacttggg aattgcccac acgcgatggg cgacccatgg aagtccgagc
     2941 gaaaccaatg cacaccctca tatgtcttcc gagaaaattg cggtagttca caatggtatt
     3001 attgaaaatt atcaagaact gaaagaaaat ctgataaaag aaggctttgt tttttcttcg
     3061 gaaacagata ccgaagttat tgcgcatctc ttgtatcgca atttacaaca taccaaccat
     3121 aatctcgtgc gcgcattcta tttaacgtta ccacaattaa aaggtgcgta ctccatagct
     3181 gttttggtta agggagaaaa aaatcgaatt ttagctgctc gacatggtag tccactggtc
     3241 attggtatcg gtattggaga gcattttgtt gcatccgata ttatagcgct gctacccgta
     3301 acacaaacat ttgtcgttct tgaagatggg gatgtcgctg aaatttttaa agatcacttt
     3361 caagtttatg ataccactgc gcagccagtg catcgcccgc ttaaacaggt aaaaatcagc
     3421 ttacaacatg cagaaaaagg cgaatttcgc cattttatgc aaaaagaaat ttttgagcag
     3481 cctcgggcat tatccgacac cttaagtggc agaattcaag cgcaccaaat taacgcttct
     3541 atttttggca cacgtgccag tgatgttttt cctaaggttg agcatgttca tattgtcgct
     3601 tgcggcacca gttttcatgc gggtgaagtt gcaaagcatt ggattcaaaa tttattgggt
     3661 gttccctgct cggtagaaat tgcaagcgaa atgcgttatc agcggcaggt ggtgctgccc
     3721 aacacgctgt ttttgtgttt atcgcaatct ggagaaacgg cagatactat cgccgccttg
     3781 cgttctacta aagaaaataa taattatttg gcaagattat gtatttgcaa tgtggcagaa
     3841 agctctttag ccagagaagc ggatattact ttcttaaccc atgcaggtcc agaaattggt
     3901 gtggcctcca ccaaggcatt tataacccaa ctcattgctt tgttattatt tacgtttagc
     3961 ttaggaaaag aaaagagcta tcaacccaag gcagaattat taaaaacttt ggcgaatgtt
     4021 cctgatttga ttgaagttac tttacaattg gatgagaaaa ttcgcgagtt gagtaaatta
     4081 tttgctgata aaaagaatgc gttattttta ggccgaggtg cactttatcc tattgcaaaa
     4141 gaaggtgctt taaaactaaa agaaatttct tatatacatg ctgagagtta ccccgccggg
     4201 gaattaaagc atggtgcatt ggcacttgtg gacaaagaca tgcctgtggt tgcattagca
     4261 tgcaatgatc atttattaga caagctgcgc tctaatttag aagaagtacg ggcaagacat
     4321 ggtcatttaa ttgtttttgc aaccgaagat acaaaactca ccttagacgc cccaggtatc
     4381 gttgttgaat tgcccagcgt acctacggaa ttggcccctg tggttatgac aatacctatg
     4441 caactcttgg cctatcatgt tgcggttttg aaaggcacgg atgtggatca gcctcgcaat
     4501 ttggccaaat cggttaccgt agagtaatgt gtggccgtta tgt
//
LOCUS       DASTIB010000027         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1191799, whole genome shotgun sequence.
ACCESSION   DASTIB010000027 DASTIB010000000
VERSION     DASTIB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191799"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFP93_00435"
     CDS             <1..239
                     /locus_tag="VFP93_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008988205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843104.1"
                     /translation="PTVHLFLQETNPEVLQIAISSKDITPLLPNPNAKEYLQVGAFYI
                     VDPQGNIMMYYPGDLTMKSVLTDLKRLLKISKIG"
     gene            243..1238
                     /locus_tag="VFP93_00440"
     CDS             243..1238
                     /locus_tag="VFP93_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9843105.1"
                     /translation="MDSKITLSAFFLAIVVIMLGAYTRLTDAGLGCPDWPLCYGSWFV
                     PYDVNSLLQAQAYFPNTTVDTIKALTEMIHRYCATLLGILVLIIGILTWKNQANTQQK
                     TLAALLTMMVILQGLLGMWTVTLKLFPLVVMAHLVGGLCTLSLLWTLWHVQNNFNLKK
                     PSESTHKFVIAGLILLFIQIILGGWTSANYAALSCPNFPLCTTKFLPKWDVTAFDIWS
                     AIRLENPLTFLSLEARQTIHMLHRLGAVCVLCIFCIISRKISKYASNLPHPTQPRLNL
                     LNKILIFLVFAQLGLGMLNVILLLPLHIALLHNMGAFFLLIITLRIFFILPKRSI"
     gene            1238..2125
                     /gene="cyoE"
                     /locus_tag="VFP93_00445"
     CDS             1238..2125
                     /gene="cyoE"
                     /locus_tag="VFP93_00445"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9843106.1"
                     /translation="MLRLFQDYLELTKPKVVLLMLLTTWVGMLLAAPPDNPPWKLMFL
                     STIGIAFCAGAAAIFNHLIDRKIDTLMKRTHNRPIAKGRISVTKALSFSLVLALLGLF
                     ILAFYVNPLTAVLTAGTVLGYAIVYTIFLKHTTPQNIVIGGLAGATPPLLGWTAITND
                     LHAHALLLVLIIFAWTPPHFWALAIYKHHEYAKAKVPMLPVTHGIPFTKLNITLYTIL
                     LMSCTVLPFLAGFMGWIYLISCSLLGCVFLGFVLVLYKSSDPKIALQTFLYSIFYLMA
                     LFISLLLDHYCVYFIKSTL"
     gene            2122..2745
                     /locus_tag="VFP93_00450"
     CDS             2122..2745
                     /locus_tag="VFP93_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9843107.1"
                     /translation="MKWHHKILFLTFSFIVIGLIAGVWKEKNKAASETPTLIAGTFLR
                     ESQNFPSFNLLDMYHQPFSELNFIGNWNIVFFGYTQCPDICPHTLHLLANLKKRPDLQ
                     WLNLIFISINPERDSPEQLKKFLEQPTFSHAKIHGLTGNKSLIHALASEIGLFIEQQT
                     NEQQPIGHSGTVLVINPKGEIQALLTHIQDELILAHDLKRIMRFKKT"
     gene            complement(2775..>3562)
                     /locus_tag="VFP93_00455"
     CDS             complement(2775..>3562)
                     /locus_tag="VFP93_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843108.1"
                     /translation="MHEWLGQIAQAGLKIKDDGLGEILLKVKNTKPKSQENAIHYLMS
                     WAAKVITTTTVIFAFTAMTNPIVGAFVGIATYFGTRDATAFGTFDLAEWFWKEGRWSI
                     TDKFLTLKNLSFKESLKATALLVCTGALAYMGAQAVFASVIGLTVLAGLNIVAVQAIA
                     WGASLWSFAASVIGLSGAIRVLTGFGFHNNQIDPIEMQSATLPTLEEGTLAKLNHLKQ
                     KYNRALRDQELLHNPGLRFTKPANESVTQEADTHASTLKKKAL"
BASE COUNT         1028 a          709 c          631 g         1194 t
ORIGIN      
        1 atcccactgt tcatcttttt ttacaagaaa ccaacccaga agtattgcaa attgccattt
       61 cgtcaaaaga tataacgccc ctattaccaa atcctaatgc taaagaatat ttacaagtgg
      121 gtgcttttta tattgtagat cctcaaggaa atattatgat gtattaccct ggggatttga
      181 ccatgaaaag tgtgctaaca gatttaaaac gtttgttaaa aatttctaaa attggttaag
      241 ttatggatag taaaattact ttgtctgcat tttttttagc catagttgtg ataatgctcg
      301 gcgcttacac acgccttacc gatgcaggac ttggatgccc tgattggccc ttatgttatg
      361 gatcatggtt tgtaccttat gatgttaatt cgctgttgca ggcacaagcc tattttccta
      421 atactaccgt agatacgatc aaagcgctca ccgaaatgat tcatcgttat tgcgccactt
      481 tattaggtat tttggtattg attataggta ttttaacctg gaaaaaccaa gccaataccc
      541 aacaaaaaac tttagcggct cttttaacta tgatggtcat tttgcaaggt ttattgggaa
      601 tgtggacagt cacacttaaa ttatttccct tggttgttat ggcgcatctc gtaggaggcc
      661 tatgtacatt aagtttattg tggactcttt ggcatgtaca aaataatttt aatctcaaaa
      721 aacctagtga atctacgcac aaatttgtaa tagcaggttt aattttgtta ttcatacaaa
      781 ttattttagg tggttggacc tctgctaatt atgcagcgct tagttgtcct aattttcctc
      841 tttgcacgac aaaattttta ccaaaatggg atgttactgc gtttgatatt tggagtgcca
      901 tccgtttaga aaacccttta acttttttaa gcttagaggc gagacaaact attcatatgt
      961 tacatcggct aggtgctgtg tgtgttttgt gtatattttg tattataagc agaaaaatat
     1021 ctaaatatgc ttcgaattta ccgcatccta cccaaccacg ccttaaccta ttaaataaaa
     1081 ttcttatatt tttagtgttt gcacaattag gactaggaat gctaaatgtg atcttattat
     1141 tgcctctcca tattgcatta ttgcacaata tgggcgcctt ttttctgtta attattacgt
     1201 tgagaatttt cttcattttg ccgaaaagaa gtatttaatg ttaaggcttt tccaagatta
     1261 cctagaatta acaaagccta aagtggtttt gttaatgtta cttacaacat gggtaggcat
     1321 gttgcttgcc gctccacccg acaacccacc ttggaaatta atgtttttaa gtacgatcgg
     1381 tattgcattt tgcgctggag cagctgcaat ttttaaccat ttgatcgata gaaaaatcga
     1441 tacgttaatg aaaagaacgc acaatagacc cattgctaaa ggcaggattt ctgttacaaa
     1501 agcgttgagt ttttcattgg tgttagcact cctgggtttg tttattttag cattttatgt
     1561 caatccatta accgctgtac taaccgctgg tactgtacta ggttatgcca ttgtttatac
     1621 cattttttta aaacacacta caccacaaaa tattgtcatt ggtggattag caggcgcaac
     1681 tccgccttta ctagggtgga ccgcaattac caatgacttg catgcgcatg ctctattatt
     1741 ggtactcatc atttttgcgt ggacccctcc ccatttttgg gcgttagcga tttataaaca
     1801 ccatgaatat gcgaaagcaa aagtacctat gttaccggta acacatggta tacctttcac
     1861 aaaactcaat ataacgcttt acaccattct attaatgagc tgtactgtac tgcccttttt
     1921 agccggcttt atgggttgga tttatcttat atcgtgtagt ttactaggat gcgtattctt
     1981 aggattcgtc cttgttttat ataaaagttc cgatcctaaa attgccttgc aaaccttttt
     2041 atattctatt ttttatctaa tggcattgtt tattagttta ttgctcgatc attattgcgt
     2101 ttattttata aagagtacct tatgaagtgg caccacaaaa ttttattttt aacttttagc
     2161 ttcattgtca ttggattaat agcaggtgtt tggaaagaaa aaaacaaagc tgcatctgaa
     2221 acgccaacgt tgattgctgg cactttttta agggaatcac aaaattttcc gagctttaac
     2281 ctgctagata tgtatcatca accttttagc gaacttaatt ttatcggcaa ctggaatatc
     2341 gttttttttg gttataccca gtgtcctgat atctgcccac atacgttgca ccttttggca
     2401 aaccttaaaa aaagacccga tttgcaatgg ctaaatctta tttttattag tattaacccc
     2461 gaacgcgatt ccccggagca acttaaaaaa tttttagagc aacccacgtt ttcccatgca
     2521 aaaattcatg gacttaccgg taacaagtca ctaatccatg ccttagcttc tgagataggg
     2581 ctttttattg aacaacaaac aaacgaacaa caacctattg ggcatagcgg cactgtactt
     2641 gttataaatc caaaaggaga aatacaggcg ttgttaacac atattcaaga cgaacttata
     2701 ttggcacacg atcttaaaag aattatgcgt tttaaaaaaa cgtaatatgc gaaggatagt
     2761 ccttcgcatt aaagctacaa agcttttttc ttcaatgtgg atgcatgcgt atcagcctct
     2821 tgcgtaacac tttcgttagc cggcttggtg aatcgcaatc ctggattgtg caataactct
     2881 tgatctctaa gtgcacgatt gtatttttgc ttgagatggt tgagttttgc caatgttccc
     2941 tcttccaacg taggtaacgt agcagattgc atttctatag gatcaatttg attattgtgg
     3001 aaaccaaatc cggttaaaac acgaatcgct cccgacaaac cgataacgga tgctgcaaat
     3061 gaccacaagg atgcgcccca tgctatggcc tgcacagcta cgatgttaag ccctgctaaa
     3121 acggttaagc caatgacact tgcgaacaca gcttgggcac ccatatatgc caacgccccc
     3181 gtgcacacca ataaagctgt tgcttttaaa ctttctttga aactcaaatt cttcaacgtt
     3241 aaaaatttat cggtaatact ccatctgcct tctttccaga accattcggc cagatcaaaa
     3301 gttccaaagg ccgttgcatc gcgtgtgcca aaatatgtag caattccaac gaatgctcct
     3361 actatcggat tggtcatcgc agtaaacgca aaaataaccg tcgtcgtcgt aataactttt
     3421 gcagcccaac tcattaagta atggatggca ttttcttgag acttcggttt agtgtttttt
     3481 acttttagca gtatttcacc taaaccatcg tctttaattt taagaccggc ttgtgcaatt
     3541 tggcccaacc attcgtgcat gc
//
LOCUS       DASTIB010000028         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1325018, whole genome shotgun sequence.
ACCESSION   DASTIB010000028 DASTIB010000000
VERSION     DASTIB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325018"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VFP93_00460"
     CDS             complement(<1..1364)
                     /locus_tag="VFP93_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET9843109.1"
                     /translation="MFMFFQKIVITNPFFKKLFGHITQCLIKASTLPYYFALALGSLT
                     ASLSFSLFLEVPTFNATVVFCFFTLFFAGFLLKKWQIWWVFICLVGFNGVLLTGTWLA
                     AHRLHVDETIFAPHNILLGEIDSIPSYSAHAIKFDFKVNKYCQNQHCTPIKTRLSLIL
                     SDLSYSLKKGDRVQLTAKLKKPYTYANPGSIDQERQNFLKNIQAYGRVLTIEVVKRAK
                     LPSLRSLLLDHVKAKYADSPFQGIILALLVGDQSLVTKKQWEIFRRTGTSHLMAISGL
                     HIGLMAGFAFMCGFYFWNCTSWAARFSAQSVACIFAMVSSIFYSYLAGWSIPTQRACI
                     MVFLCMWVLLTRHWLSRLQIFCIALWIVLIVDPFASLRAGFWLSFFAVGILLCSSWIQ
                     SHTTWKKWIAPQLWVFVGLLPLTIFWFTEISLWSPLVNLFAIPWMSFVIVPCLFFDTI
                     LTLI"
     gene            1302..1868
                     /locus_tag="VFP93_00465"
     CDS             1302..1868
                     /locus_tag="VFP93_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016962364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET9843110.1"
                     /translation="MAKKFFKKWISDHNFLKEHKHLKVFGQLIHDPNLWHLNRYSVAT
                     AFSVGLFVAFIPVPFQMVIAGALAIIVHANLPISIALVWLTNPLTMGPIFYFCYRVGA
                     WILVREPRRFKLEFGYDWLIDNMYAIGIPFLLGCFITGSFLAISSNFLIRMIWRYSVS
                     KNWRERRLKRKQKRELKQSKKRRKSKPS"
     gene            complement(1895..2578)
                     /gene="lolD"
                     /locus_tag="VFP93_00470"
     CDS             complement(1895..2578)
                     /gene="lolD"
                     /locus_tag="VFP93_00470"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HET9843111.1"
                     /translation="MITDPILRCQGLHKIYKDAEKIIPVLSGIDFQVAPNEKIAIVGK
                     SGSGKTTLIHLLGGLDRPSEGKIFFENTNIHHLSDRKLGFLRNHLFGYIFQFHHLLPE
                     FTALENVAMPLLIRGIPIAQARERSDKALMQVGLDDRKTHKPNKLSGGERQRVAIARA
                     LVTKPKCIFADEPTGNLDQATATKIYDLMIQLAEEVGTSFVVVTHDDDLASRMDRVVR
                     LEQGQLQQL"
     gene            complement(2568..>2752)
                     /locus_tag="VFP93_00475"
     CDS             complement(2568..>2752)
                     /locus_tag="VFP93_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HET9843112.1"
                     /translation="FGVHFLSEEVYFISRLPSQLLMKDVMLVASASLILSLLATIIPA
                     YRAANVQPAEGLRYDH"
BASE COUNT          899 a          538 c          514 g          801 t
ORIGIN      
        1 aatattagcg ttaaaattgt atcgaaaaaa agacaaggca ctataacaaa gctcatccaa
       61 ggaatggcaa ataaattcac aagcggtgac cacaaactaa tttctgtaaa ccaaaaaatc
      121 gtcaatggca aaagccctac aaacacccaa agttgtggag caatccattt tttccatgta
      181 gtatgcgatt gaatccagct ggaacacagt aaaattccaa ccgcaaaaaa ggataaccaa
      241 aaccctgcac gaagactggc aaatggatcc acaattaaaa ctatccaaag cgctatgcaa
      301 aatatttgta accgactaag ccaatgtcgt gttagtagga cccacatgca caaaaaaacc
      361 ataatacacg ctctttgcgt tggaatggac caacctgcca agtacgaata gaaaatactg
      421 cttaccattg caaaaataca ggcaaccgat tgcgcactaa aacgcgcagc ccaggaggta
      481 cagttccaaa aataaaaacc acacataaaa gcaaaacctg ccattaatcc aatatgcaaa
      541 cccgatatcg ccattaaatg actggtaccg gtgcggcgga aaatttccca ttgttttttt
      601 gttactaaag attgatcgcc aactaataaa gcaagaataa ttccttgaaa gggagaatct
      661 gcatatttgg cttttacatg atccaataaa agtgatcgta aactgggtaa ctttgcacgc
      721 tttaccactt caatagttaa aacacgtcca taggcttgga tattttttaa aaagttttgc
      781 cgttcttgat ctatactacc tgggttcgca taggtgtaag gttttttaag ctttgctgtg
      841 agttgtactc tatctccctt ttttagggaa taagacaaat ccgataaaat taagcttaag
      901 cgtgttttga taggcgtaca atgctgattc tggcaatatt tatttacctt aaaatcaaat
      961 ttaatagcgt gtgcactata agaaggaatc gaatcaatct cacctaatag gatattgtgc
     1021 ggcgcgaaaa ttgtctcatc tacgtgtaac ctatgagcag ccaaccatgt cccggttagt
     1081 aaaacaccat taaatcctac caaacatata aaaacccacc aaatttgcca tttttttaat
     1141 agaaatcctg caaagaacaa agtaaaaaaa caaaaaacaa cggttgcgtt gaatgtgggg
     1201 acttccaaaa atagactgaa gcttaaactc gctgttaaac tacctagagc aagagcaaaa
     1261 taatatggaa gagtgctagc ttttattaag cattgggtaa tatggccaaa aagtttttta
     1321 aaaaatggat tagtgatcac aattttttga aagaacataa acatttaaag gtttttggtc
     1381 aacttattca tgatcccaac ctctggcatt taaatcgcta ttccgtggca actgcgtttt
     1441 ccgttgggtt atttgtcgca tttattccag ttcctttcca aatggtcatt gctggcgcac
     1501 ttgcaattat tgtgcatgca aatttgccta tttctattgc tttagtgtgg ttaacaaacc
     1561 ctttaaccat gggccccatt ttttattttt gttatcgtgt tggggcttgg attttagtac
     1621 gtgagccaag acgattcaaa ttggagtttg gttacgattg gcttatcgat aatatgtatg
     1681 ctatcgggat cccttttcta ttgggatgtt ttattaccgg tagctttttg gccattagta
     1741 gtaatttttt aataagaatg atctggagat attcggtatc aaaaaattgg agagagcgac
     1801 gtttgaaacg taagcaaaaa agagaactaa aacaatccaa aaaacgtcgt aaatctaaac
     1861 cgtcataaag ttgttactta atacattcac ctggttaaag ttgttgtaat tgaccttgtt
     1921 cgagtcgcac cacacgatcc atgcggctcg ctaaatcatc atcgtgggtt accaccacaa
     1981 agcttgttcc cacttcttct gccaattgga tcataagatc ataaattttt gtcgcggtag
     2041 cttgatccaa atttccggta ggctcatccg caaaaataca cttgggtttt gtgactaaag
     2101 cacgcgctat ggcaacgcgc tgtcgttctc ctcccgaaag tttattcggt ttatgtgttt
     2161 ttctatcgtc aagtcctact tgcattagtg ctttatcact gcgttctctt gcctgtgcga
     2221 taggaatgcc acgaataaga agtggcatag caacattttc taatgctgtg aattccggaa
     2281 gtaaatgatg aaattgaaaa atgtagccaa acaaatgatt gcgcaaaaaa cccaacttgc
     2341 gatcggacag atgatgaata tttgtgtttt caaaaaaaat tttgccttca ctgggtctgt
     2401 ctaaaccccc caataaatga atcagcgtgg ttttacccga accggatttg cctacaatgg
     2461 caattttctc attgggtgcc acttggaagt cgatgccaga aaggactgga ataatttttt
     2521 cggcatcttt ataaatttta tgcaatccct gacaacgtag aataggatca gtgatcataa
     2581 cgtaatcctt cagcaggttg tacgttggcg gctcgataag ccgggatgat agtagcaaga
     2641 aggcttaaaa ttaatgaggc ggaagcaact aacattacat ctttcattaa taattgcgaa
     2701 ggtaagcgac ttataaaata aacttcttcg gatagaaaat gtaccccgaa ta
//
LOCUS       DASTIB010000029         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_176828, whole genome shotgun sequence.
ACCESSION   DASTIB010000029 DASTIB010000000
VERSION     DASTIB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176828"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFP93_00480"
     CDS             <1..1067
                     /locus_tag="VFP93_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843113.1"
                     /translation="IMRLSKNQPCTLMLDIGNIETGHAMGFARNKHGIWLHDSNHGLI
                     FFDAKNPEYKENFKQFFQEYMKSYKDCYASIKIRNTSIAYLIKDAMDISQGYLDSFKQ
                     ITNLICSSDDWNAAIKTAAECRDVLKRFISDDKSEKEIFSQLLSNPEVMNRILLYKNT
                     IKDDLKLKRDPDLQNQLDRLEKLTPILQNLDALKFYDLFKNAQDQLNTISTDVYKKGD
                     PKYIKEFQQTIINLETSAKKLKKLKMLSSNKEQFNASINKVFKALSSIEKNASALKSL
                     PPIKNPSPLTKSIPNILSILDSFKKIPHHLPAKQPKKNILNQLSHKQNGFFTHLKSTL
                     SNLKPNEKSNKNKSPSRFNQ"
     gene            1219..1866
                     /locus_tag="VFP93_00485"
     CDS             1219..1866
                     /locus_tag="VFP93_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843114.1"
                     /translation="MVEYDFYGMTLLTRLAYVGSDVTQAAEILKKNPEAVNARGKFLN
                     ETPLLAAVTGGTASTTFIKLLLEYGARLDVEYLAGRTVLHCVAYSTNIEHLDLLKGKA
                     GFPDINYITNELYTPLHAVCMLGHNAATMECRKKMAAWLLDNGAKIWRNTYGNTPIQE
                     ARNQGLNELADFIEAYTPIHQQANNYLPLSNSVESKKDDPTELKDNNARLRTINF"
     gene            1979..2638
                     /locus_tag="VFP93_00490"
     CDS             1979..2638
                     /locus_tag="VFP93_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843115.1"
                     /translation="MLEYDFYGRTPLARLAFLGNEIDTAKAILEHCPETVNARGKFFG
                     ETPLLTAVSGGRAKAEFIELCIQYGAELDATYSNGKNVLHCIANSTNMEHLNVLKDKP
                     GFPSVNSLTNDFQTPLHAVCMQGNSAATLESRKKMAVWLLDNGARPFYKNASQRTPIE
                     EAQAQGLAELADFIESYVPPHKQANTFLPMLNSLRSLHDNAVEPRISKNKNPSLRIVN
                     F"
BASE COUNT         1010 a          480 c          449 g          825 t
ORIGIN      
        1 agattatgcg tttgagcaag aatcaacctt gtactttaat gctagatatt ggcaatattg
       61 agacagggca tgctatgggt tttgcccgta ataagcatgg aatatggctg catgattcaa
      121 atcatggctt aatttttttt gatgccaaaa atcctgaata taaagaaaat tttaaacaat
      181 tttttcaaga atacatgaaa agctataaag attgttatgc ttcaataaag ataagaaata
      241 cttctattgc ttatctcatt aaagatgcaa tggatatttc tcaaggttac ttagatagct
      301 ttaaacaaat taccaattta atttgtagtt ccgatgattg gaatgcagct atcaaaacag
      361 ctgctgaatg ccgtgatgtg ctcaaaaggt ttatctccga tgataagtct gaaaaagaga
      421 tattttctca attactttca aatcctgaag tcatgaatcg tattttatta tacaaaaata
      481 ccataaaaga tgatttaaaa cttaagagag atccagattt acaaaatcag ctagatagac
      541 tcgaaaaatt aacacctata ttacaaaatt tggatgcatt gaagttttat gatcttttca
      601 aaaacgcaca agatcaactt aacactattt ccacggatgt atataaaaaa ggtgatccaa
      661 agtatattaa agaattccaa caaactataa ttaatttaga aacatctgct aaaaaattaa
      721 aaaaattaaa gatgctatct tccaataaag aacagtttaa tgcttcaata aataaagttt
      781 ttaaagcatt atcctctatc gaaaaaaatg cttccgcatt aaagtctttg ccgcccataa
      841 aaaatccatc accactgaca aaatctattc caaatattct ctcaattttg gatagtttta
      901 agaaaattcc tcatcattta cctgcaaagc aacccaaaaa aaatatacta aatcaattat
      961 cgcataaaca aaatggattt tttacacatt taaaaagcac attgtcaaat ttaaaaccaa
     1021 atgaaaaaag taataaaaac aaatctccta gtagattcaa tcagtaaagc tagcaagctt
     1081 aacagactac ctcccccatc aaacttagta cgtaaactaa aaaaaatata ttttttgcgt
     1141 gaaacccgtg aatcggaatt caaaaatatt gactcaaaag ctaggaacga acgatagttt
     1201 aatcttttgg agttttttat ggttgaatat gatttttatg gtatgactct ccttacccgc
     1261 ttagcctatg ttggctctga cgttacgcaa gcagcagaaa tactaaaaaa aaatcctgaa
     1321 gctgtaaacg caagaggcaa atttttaaat gaaactccat tacttgctgc agtgacgggt
     1381 ggtacagcaa gcactacttt tattaagctt ttgctcgaat atggtgcaag acttgatgtg
     1441 gaatatttgg ccggcagaac tgttttacat tgtgttgctt attctacgaa tatagaacat
     1501 ttagatctac taaaaggtaa ggctgggttt ccagatatca attacattac aaacgagctt
     1561 tacactccac ttcatgcagt atgtatgcta ggtcataatg ctgcaacaat ggaatgccga
     1621 aaaaaaatgg ctgcctggct attggataat ggagcaaaaa tttggagaaa tacttacggt
     1681 aacacaccaa tacaagaagc ccgtaaccaa ggtttgaatg aattagcgga ttttatcgaa
     1741 gcgtatactc ctattcacca acaagcaaat aactatttgc ctttgtcaaa ctctgtagaa
     1801 tccaagaaag acgatccaac agagcttaaa gataataacg cacgtttgag aacaataaat
     1861 ttttaataaa attccctata attattttat aacaagatca agtaccattt taagtatgat
     1921 ttaacttttg agtaaaaagc gataaaaaac gtgtattcaa attatttagg agatttgtat
     1981 gttggaatat gacttttatg ggaggactcc gctagcacgc ttagcctttc ttggtaacga
     2041 aatcgatacc gcaaaagcca tactggaaca ttgtcccgaa acggttaacg cgcgcggcaa
     2101 attttttggt gaaacacctt tgctaactgc agtctcaggc ggcagagcta aagctgaatt
     2161 tattgaacta tgcattcaat atggcgcaga actcgatgct acctatagca atggtaaaaa
     2221 tgttttgcat tgtattgcta attctaccaa tatggaacat ttaaacgttt taaaagataa
     2281 gcccggattc cccagcgtga actcgttaac gaacgatttt caaacaccac ttcatgccgt
     2341 atgtatgcaa ggaaatagtg ccgcaacttt ggaatcacga aaaaaaatgg cagtatggct
     2401 actggataat ggtgcgagac ctttttataa aaatgcttca cagagaacac caatagaaga
     2461 agcgcaagcc caggggttag ctgaattagc tgattttatc gaatcttatg ttccacccca
     2521 caaacaagca aacacttttt tacccatgtt aaattcttta cggtcattgc atgataatgc
     2581 ggtagagccg cgaattagta aaaataagaa cccgagctta agaatagtta atttttaagt
     2641 tgagtcgaat ttataactat ttcatgtgaa aagctcttgt tgcggaaact gaacagtttc
     2701 cgcattaaaa attttctata aaaatcaata atttaaatat aatccgccta ccactagcat
     2761 tatt
//
LOCUS       DASTIB010000030         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_787880, whole genome shotgun sequence.
ACCESSION   DASTIB010000030 DASTIB010000000
VERSION     DASTIB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787880"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1318
                     /locus_tag="VFP93_00495"
     CDS             128..1318
                     /locus_tag="VFP93_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843116.1"
                     /translation="MQNSEAKNFEGRTALHLAVEKNDVEQVIKLIRLGYDVNSTDNNN
                     FTPLHLAAIVGSTKIARLLLKEGANVNVKEKQFQHTPLHYAIINKNLKIAGYLIEANA
                     EVDAENHQGRTSLHFASHDNYVEGINLLLKNKADLLVKDDKGFIPLHYAQFCNHTNAI
                     DMLMQAAKQAFHMAVYLRQMTKAEQLLNSGIIDINALDDKGYTALHVTSICGFSAIAQ
                     MLIQKKADVNANDSQSRTPLHAAISSGSHDIVRLLVKHEADINAKDNKLQTPLHYAIL
                     KKRSNLQNFLLELAVTTNNDKNDINFLINNGANIKDRDDNGMSPRDYDSCCKKETRTF
                     LNTFIQNITNQQGEDLDVKPVIEELNSIQIQEGPQPLIFSQSSEIPETSSIKTDEKIQ
                     KIRP"
     gene            1395..2012
                     /locus_tag="VFP93_00500"
     CDS             1395..2012
                     /locus_tag="VFP93_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843117.1"
                     /translation="MKTTSGNKDFLGCSALHLAVEAGDIAKVRELLATKNIDVNSSAN
                     NKYTPLHVAAIFGKTEIAQVLIQEGKADIEAKDYVEHSPLHLAIINKELELAKHLLNA
                     GANINATNHKGITPMHYAAMNNHVESIQFLIENNANVNAADPNGETPLHFAKIWNNRE
                     AIQLLTKLVEQETKPTLLFSFSQLTESLSSMFYKKPTEDQQKPSP"
BASE COUNT          854 a          384 c          400 g          711 t
ORIGIN      
        1 cgcgattaat aattttgcaa attactttac aaataaggta aacattttta aactctgtaa
       61 gcgatcgtta aagcgatcgc ttattaattc cacctattcg taatatgaaa attataaaaa
      121 ggtatttatg caaaattcag aagcgaaaaa ttttgaaggt cgtactgcat tacatttggc
      181 agttgaaaag aatgatgtag agcaagtaat taaattgata cgtttaggct acgatgtaaa
      241 tagtactgat aataataatt ttacaccgct tcacttagca gcaatcgttg gctctacaaa
      301 aatagcgcgc ctacttttaa aagaaggagc taatgttaac gttaaagaaa aacaattcca
      361 acatactcct ttgcattacg cgattataaa taaaaattta aaaattgcag gttatcttat
      421 tgaagcaaac gcagaagttg atgctgaaaa tcatcaaggt cgaacttctc tccattttgc
      481 ctcacatgat aactatgtag aaggcattaa tttattgctt aaaaataagg cagatctttt
      541 agttaaagat gacaaaggtt ttattccttt gcattacgcg caattttgta atcataccaa
      601 tgctatcgat atgcttatgc aggctgctaa acaggcattc catatggcgg tttatttgcg
      661 gcaaatgact aaagctgaac aattgctaaa ctcaggcatt atagatatta atgctctgga
      721 cgacaaaggt tatacagcgt tacatgttac atcaatatgt gggttttctg ccatcgctca
      781 aatgttaata caaaaaaagg cggatgttaa tgccaatgat agtcaatcac gaacaccttt
      841 acacgctgca ataagcagtg gatcgcacga tatagtgcgt ctgctcgtta aacacgaagc
      901 ggatattaac gctaaagata ataaattaca gactccgttg cactatgcca ttctaaagaa
      961 aagatcgaac ttacaaaatt ttttattgga actagctgtg acgacgaata atgacaaaaa
     1021 tgacattaat tttttaataa acaatggtgc aaatataaaa gatagagatg acaatggaat
     1081 gtcacctagg gattatgata gttgttgcaa gaaagagact agaacgtttc taaatacttt
     1141 tattcaaaat attaccaacc aacagggtga agatctagat gtcaagcctg ttattgaaga
     1201 gttaaatagt atacaaatac aagaaggacc tcaaccttta atattttcac aatcatctga
     1261 aatacccgaa acttcatcca taaaaacgga tgagaaaata caaaaaattc gcccttaata
     1321 aagaatctaa gctgtaggat tgaacttaca tagtaattgt gcgttattat tgaaatttta
     1381 atataaggaa ttgtatgaaa acgacatcag gaaataaaga ttttttaggt tgtagtgcat
     1441 tgcatcttgc agttgaagca ggggacattg caaaagttcg cgagctcctt gcaacgaaaa
     1501 atattgatgt aaatagctct gccaacaata aatatacacc acttcatgtt gcagcaatat
     1561 tcggtaaaac agagatagcg caagtgttaa ttcaagaagg aaaagccgat atagaagcaa
     1621 aagattatgt tgagcattct ccattacatt tggctattat caataaagaa cttgaacttg
     1681 caaaacactt gctcaacgct ggcgcgaata ttaacgctac aaatcacaag ggtatcacgc
     1741 cgatgcatta tgctgctatg aataatcacg ttgaaagcat tcaattttta attgaaaata
     1801 atgccaatgt aaatgcagct gaccctaatg gagaaactcc actgcatttt gctaaaattt
     1861 ggaacaatag agaagcaatt cagttgttaa ccaaacttgt agaacaagag acaaaaccaa
     1921 cgttattatt ttcgttttca caattaacgg aatctctgtc cagtatgttt tataaaaaac
     1981 caacagaaga tcagcaaaag ccaagtcctt gaagctataa atcaaactct aaggtgtttg
     2041 ccttggagtt ttttgagatt gggtttttac aactactata gatttaaaga cataacgcac
     2101 aatattcctg agtacgccta tactcaagag tgtgttgggc ggtacctata gcattataaa
     2161 attaaatatt aatttcattt agataactta tctgttcagt aaaagtttta taatcaatct
     2221 tggctataaa cccatttttc ccatgcttgt atattttttg cagaaaagtt ttgattgctg
     2281 atatttttca aaataagata ggctagctca tgtacattcg gctgtttcat ttttagattt
     2341 tcaattaaa
//
LOCUS       DASTIB010000031         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_996155, whole genome shotgun sequence.
ACCESSION   DASTIB010000031 DASTIB010000000
VERSION     DASTIB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996155"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1934)
                     /locus_tag="VFP93_00505"
     CDS             complement(150..1934)
                     /locus_tag="VFP93_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012461184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9843118.1"
                     /translation="MNIPITYRHVYAYIASHIKAFPFAILTMLGVALIWAISISVKPY
                     LIKIILNRLSISAQADVFVYLATPILLYLGMNLLLTISYRFYGYFVEIKMIPKLRKQM
                     ANDALGKLIEKSHTFYQNNFSGSLTNKVNDLTRSVPEILQIIFDKFISHVLALSIAIL
                     TLWQVNYIFALIMLVWNVLFSLGAMLYSKKLTLLADRWSEYGSTITGRIVDVLSNILT
                     VRLFSAKKPEKKLLNETFQQAVNAEQQLQWAYFWMWMCYSLSALLLQVLNFYFLCKGR
                     QEGWITIGDFALVLVLNISIIDYFWQITHEFSHFSKLYGNITQALRAILEKPEIEDNV
                     NANNFGVIQGKITFDTVKFHYKGAIPLFQNMSIEIAAGQKVGLVGYSGSGKTTFVNLI
                     LRLYEITNGQILIDNHDIKSLSQDFLRSQIGMIPQDPSLFHRTLLENIRYGRLDAKDE
                     EVIEAAKRAHAHEFIIKLSQGYHSLVGERGIKLSGGQRQRIAIARAVLKNAPILILDE
                     ATSQLDSLTEALIQESLWELMQGKTTIVIAHRLSTLLPMDRILVFEKGKIVEDGTHSQ
                     LLNNHNLYKSLWDSQIGGFLPDKTSEDL"
     gene            complement(2137..2364)
                     /locus_tag="VFP93_00510"
     CDS             complement(2137..2364)
                     /locus_tag="VFP93_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843119.1"
                     /translation="MAGQNYKLLLEYNKLSVNFFHYLSQRMQYEKKRSLDKLFRIDFG
                     LNRFKNRQSKFLAVLTAPKSKPMDVTHDENE"
BASE COUNT          849 a          428 c          375 g          814 t
ORIGIN      
        1 cgctatgtca tttatttatt ctcctaatta gagcaacacg actatataag atataagaaa
       61 ttatattggc tgtccttata atgtccctta taatgtcctg cacttataat gtccttataa
      121 agattatagt ggatgtcctc atagaaatat tataggtctt cggaggtttt atcgggaaga
      181 aaaccgccaa tttgtgaatc ccagaggctc ttataaagat tgtgattatt taataattgc
      241 gaatgcgtgc catcttctac aattttacct ttttcaaata ctaaaattct atccataggt
      301 agtaaggtag aaagtctatg tgcaatgaca atcgttgttt tcccttgcat taattcccat
      361 aaactttctt gaattaatgc ttcagtcaga gaatcaagct gtgacgttgc ttcatctaaa
      421 attaatatgg gggcattttt taaaaccgca cgagcaattg cgatacgttg gcgctgacca
      481 cctgagagct ttatgccacg ctctccgact aaagagtgat acccctgtga aagcttaata
      541 ataaattcat gagcatgtgc tcgttttgca gcttctataa cttcttcatc tttggcatct
      601 aaacgtccat aacgaatatt ttccaataag gtacgatgaa ataaagatgg atcttgcggt
      661 atcatcccta tttgactacg caaaaaatct tgcgataatg attttatatc atgattgtca
      721 attaaaattt gaccatttgt tatttcgtac aaacgtaaaa ttaaatttac aaatgtggtt
      781 ttaccactgc ctgaataacc tactaatcct actttttgac cggctgcaat ttcaatagac
      841 atattttgaa ataaaggaat agcgccttta taatgaaatt tcacagtatc aaacgtaatt
      901 ttcccttgaa ttacgccaaa attattcgca ttaacattat cttcaatttc tggtttttct
      961 aatattgcgc gcagagcctg ggtaatattc ccatataatt tagaaaagtg cgaaaattca
     1021 tgcgttattt gccaaaaata atcaataatg gaaatattaa gtaccagaac aagtgcaaaa
     1081 tctccaatgg tgatccaacc ttcttgtctt cctttacata aaaaataaaa atttaatact
     1141 tgtaataaaa gagccgataa actgtagcac atccacatcc aaaaataagc ccactgcaac
     1201 tgctgttctg cattaacggc ttgttgaaat gtttcattca ataatttttt ctctggcttt
     1261 ttagcagaaa aaagtcgaac agttaaaata ttggacaaaa catcaacgat tcttcctgta
     1321 attgtggaac cgtattcgga ccagcgatcc gcgagtaaag ttaatttctt tgaatacaac
     1381 atagcaccca aagaaaatag cacgttccaa accagcataa ttaatgcaaa tatataattc
     1441 acttgccaca acgttaaaat agctatcgaa agggccaaaa catgactaat aaatttatcg
     1501 aaaattattt gcaaaatttc aggcacgctt cgtgttaaat cattaacctt attggttaag
     1561 cttccagaaa agttattctg ataaaaggtg tgactttttt caatcaattt tcctaaagca
     1621 tcatttgcca tttgttttcg taattttggg atcattttaa tttcaacaaa atacccataa
     1681 aatcgatagg aaatagttaa tagcaaattc atgcctaaat aaaggagtat cggcgttgcc
     1741 aaatagacaa aaacatctgc ttgtgcgcta atacttaatc tatttaaaat tatttttatg
     1801 agatacggtt taaccgatat gctgatcgcc catataagcg caacccccaa catggtgaga
     1861 atagcaaagg gaaaagcttt tatatgcgat gcaatgtatg catatacgtg tcgataggtt
     1921 attggaatgt tcatactgct tcctaaagtg taggtaaata atagcattcc aaataaaaat
     1981 gaacaggaat tttattaact ttcttacagt attgtcacaa atgtttagtg acttcctaca
     2041 tttttgcaat ttatccaaaa agtttacatc ctcagtaacc atgcatcgta ctaaccaaat
     2101 aaatattttt atgatttata aaatgactga taaaatttat tcattttcat catgagttac
     2161 atccatgggt ttagatttgg gtgccgtaag tactgctaaa aatttgcttt gacgattttt
     2221 gaagcgattc aaaccaaagt ctattctaaa cagtttatca agtgaccttt tcttttcata
     2281 ctgcatgcgt tgggataaat agtgaaaaaa attaacagaa agtttgttat attcaagcag
     2341 taacttataa ttttgacctg ccaaaatttg gcttaaataa taagcaaaaa aatactgtga
     2401 agaactaaag agaaaacctt actgaaaaaa aaagaatgac ttttttttaa tattacgtaa
     2461 tttgct
//
LOCUS       DASTIB010000032         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1294180, whole genome shotgun sequence.
ACCESSION   DASTIB010000032 DASTIB010000000
VERSION     DASTIB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294180"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFP93_00515"
     CDS             <1..414
                     /locus_tag="VFP93_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9843120.1"
                     /translation="GAKAYLQENINLELLKKIVCKVREGEIWVDRMFVSRLLKEIEDI
                     TKVQHQEAKEIEKGVASMTPREEQIAELIATGSSNRRIAEKLCISERTVKAHLGVIFR
                     KIGISDRLQLALYMNKYHQLSSVWHPIAENKVSRE"
     gene            644..>2117
                     /locus_tag="VFP93_00520"
     CDS             644..>2117
                     /locus_tag="VFP93_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HET9843121.1"
                     /translation="MGKNKLLKWLLPCCIGLSISSQALAHTVQEAVAHALKTGPDILI
                     SANSREAIEEELREAFAGYLPTIDLNAGWGREKSENSSTRIPGQTDGSRTLWRTEFGI
                     NARQMLFDGFAVKNDVEGNMARVRAASWRVNNDASEIALDVITAFLEINLEKELVHLA
                     RQNLNAHQGTFGQIERRSESGIGRRADLDQATGRLAFARTNLLAEQANLRDAETRFLR
                     LVGIPPIELVQPESPTVGFPETLRSAIALGVNYHPELRAAVTDVSVARAAHRAAKAPF
                     YPRLDFELGFNRNHNIDGVTGDNDDFFGMLRLRWNLFRGGADLARTKATAWQIEEAKE
                     VRNRAHRQVEESVRLAWSTYVTASSQIHYFRQHVESSLSTRDAYEKQFNIGQRTLLDL
                     LDSENELFTARTAYANGKHLEILGKFRVLQATGCLLDYLGVPLPRAARIEEPTLFGFN
                     YGKPAHYYDQYNRTNYYSDAYYGAPTNTTYTVDEHHVDFGE"
BASE COUNT          655 a          431 c          463 g          568 t
ORIGIN      
        1 ggtgcaaaag cctacctaca ggaaaacatc aatcttgagc tactcaaaaa aattgtttgc
       61 aaagttcgtg aaggtgaaat ttgggtagat agaatgtttg taagccgact tctaaaagaa
      121 attgaagata tcaccaaagt acaacaccaa gaagcaaagg aaattgaaaa aggggttgcg
      181 tctatgacac ctcgagaaga acaaatagcg gaattaattg ccacaggctc aagtaaccgt
      241 agaattgcgg aaaaattatg tatatcagaa cgcaccgtaa aagcacatct tggtgtaatt
      301 tttcgaaaaa ttgggatatc cgatcgattg caattagcgc tttatatgaa taagtatcac
      361 caactctcct ctgtgtggca tcccatagct gaaaataaag tttctcgcga gtgagttaaa
      421 atcctgacct ctttatctcc gtttcttttt aaaaaaagag cagctacaat catatttgcg
      481 cgatacaacc tgactatacg attagaggct gtattatgga caactgggtt caattgggct
      541 atatcttgtc attgtgacca ataaccaagc acttaactta gttgttgcaa ggcacaaatt
      601 atgatggctc agggaaaaat taactataaa acggattcaa gtcatgggaa aaaacaaact
      661 tcttaaatgg ttattacctt gctgcatagg attaagtatt agttcgcaag ccttagcaca
      721 tactgttcaa gaggctgtgg cacatgcatt gaaaactggt ccggacatct taatttctgc
      781 taactcacgt gaagcaatcg aagaggaatt acgcgaggcc tttgcaggtt atttgccaac
      841 tatcgattta aatgcgggtt ggggtcgcga aaaaagtgaa aattcttcta ctagaatccc
      901 aggccaaacc gatggctctc ggacattatg gcgtacagaa tttggaataa atgcccgtca
      961 aatgttattt gatggttttg cagtgaaaaa cgatgttgaa ggcaacatgg cgcgtgtaag
     1021 agctgcctcc tggcgcgtta ataacgacgc atcagaaatt gcacttgatg tgatcaccgc
     1081 gtttttagaa ataaatttag aaaaagaact agttcatttg gcccgtcaaa atcttaatgc
     1141 gcaccaaggt acgtttgggc aaattgaaag acgtagtgaa agtggaattg gtcgtcgagc
     1201 cgaccttgat caagctacag gtcgtttagc atttgcacgc actaatttat tagcagagca
     1261 agctaattta agagacgctg aaacacgctt tttgcgctta gtggggatcc cgcctattga
     1321 gctagtacaa ccggaaagtc ccacggtggg attccctgaa actttacgct ctgcgattgc
     1381 tttaggtgta aactatcatc ctgagctaag agcagcggtt accgatgtct cagttgcgcg
     1441 cgcggcacat cgtgcagcaa aagcaccctt ttatcctcga cttgattttg aattagggtt
     1501 caaccgcaac cacaatatcg acggcgtcac aggtgacaac gacgacttct ttggcatgct
     1561 aagattgcgt tggaaccttt tccgtggcgg tgctgattta gctagaacaa aagctaccgc
     1621 ttggcaaatc gaggaagcaa aagaagtacg caaccgtgca catcgacaag tagaagaaag
     1681 cgtgcgctta gcttggagta cttatgtaac ggcttcttcc caaatacatt actttagaca
     1741 gcacgtagag tcttctttat ccacccgcga tgcttacgaa aaacaattta atataggcca
     1801 aagaacctta ctagacttac ttgattccga aaatgaattg tttactgcac gtactgccta
     1861 tgcgaatggt aagcatctgg aaatacttgg taaattccgc gtactacaag ctacgggctg
     1921 tttgttagat tatcttggag tgcctttacc aagagccgcg cgcatcgaag aaccaacgtt
     1981 gtttggtttt aattacggta aacctgcgca ttattacgat cagtacaatc gcaccaacta
     2041 ttactcagat gcttattatg gagcgcctac caataccaca tatacggtcg atgaacacca
     2101 tgtggatttt ggtgaga
//
LOCUS       DASTIB010000033         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1278780, whole genome shotgun sequence.
ACCESSION   DASTIB010000033 DASTIB010000000
VERSION     DASTIB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278780"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFP93_00525"
     CDS             complement(<1..921)
                     /locus_tag="VFP93_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HET9843122.1"
                     /translation="MRHLVFRSLFLFGYAFFPLIVFSKWDITPSLTLQELYSDNINLN
                     YERNSEFVTEVAPLINFDNKNTLHPIFGFYRVQGLYFAKSNRENRIYQQALVNTQTSF
                     LHRSLKLNAIADHTQQILFPQTEIGVDNIHGSRRSDVSRVMVGPDFIHPLSKWLKSFW
                     SYRYGQVYFHEDNVENSHLQQATWNLGSRNTTHFDFSSNVDWEHTQQDTLRIAESFNA
                     MATLGYRLGPKFRAYAAGGYEEHEGQGLNNALDGTRWHIGAQFAPTRNTFLDGNWGRR
                     SFGETYNLHALWKRKKQSFLLTYQEDITNYF"
     gene            complement(945..1691)
                     /locus_tag="VFP93_00530"
     CDS             complement(945..1691)
                     /locus_tag="VFP93_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843123.1"
                     /translation="MINDLPQRKSKPNKDPHKNLIIPDLSLGKPIDLKQIQLEGYLEF
                     PYDQGSIAEKYRGIKWEILNHHRFPSRGKTLLIASASVGEGKSAAAFNLAVSMSLERD
                     WKSILVDTSGNANSITSLLNLNQCKGLTDYLVGDINLEMALYETSQAKCYVVPIGQQI
                     HLRSELIASTKMVDFFQKIQEFIPSSFVILDSRAIRSYADCKVLSTRVDRILMVVEAS
                     VTPKLQVEEALYFLPKEKLIGVIMNKDPWK"
     gene            complement(1688..>2182)
                     /locus_tag="VFP93_00535"
     CDS             complement(1688..>2182)
                     /locus_tag="VFP93_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HET9843124.1"
                     /translation="QSMEEKVDQIIDAETKLSNLNRDYSMNKAQYNALLEKRDAAKLV
                     EDIDGTSENLTFRIIEPPYASSLPVWPKRNLFNLVVLVASLLGGVASTFVLAQWKPAF
                     YDKISLEKALNLPVLAVIPQEKNHIKRKISLKLNLLYVICILGLWGGFMLLYYMQRQG
                     VMPT"
BASE COUNT          665 a          423 c          389 g          705 t
ORIGIN      
        1 aaaataattg gtaatgtctt cttgataggt tagcaaaaag ctttgttttt ttcgtttcca
       61 caaagcatgc aaattgtaag tttccccgaa cgagcgccgt ccccaatttc catccaaaaa
      121 agtgttccgg gttggggcaa attgcgcccc aatgtgccac ctcgttccat ctaaagcatt
      181 attaagacct tggccttcgt gctcttcgta tccacctgct gcataagctc taaattttgg
      241 ccccaatcga tagcctaagg tagccattgc attgaatgat tcagcaatac gaagcgtatc
      301 ttgttgggtg tgttcccaat ctacattact tgagaaatca aaatgggtag tatttcgact
      361 acccaagttc caggttgctt gctgcagatg ggaattctcc acattatctt catgaaaata
      421 aacttggcca taacgatacg accaaaaaga ttttagccat ttgcttaaag gatgtataaa
      481 atctggtcca accattacgc gcgaaacatc ggacctgcgt gagccatgta tattatccac
      541 gccaatttcg gtttgaggaa acaagatttg ttgggtgtga tctgctattg catttaattt
      601 taacgatcga tgcaaaaagg aagtttgtgt gttcacaagt gcttgttgat aaattctatt
      661 ctcacgattt gacttagcaa aatataagcc ttgcacacga taaaaaccaa aaattggatg
      721 caaagtattt ttattgtcaa aattaatgag aggtgctact tctgttacaa attcggagtt
      781 tctttcataa tttaaattaa tgttgtcgga gtataattct tgcaaagtaa gagatggcgt
      841 tatgtcccac tttgagaata cgatgagcgg aaaaaatgca taaccaaata gaaacaatga
      901 acgaaaaacc aaatgccgca tagcttccct gcatagtaat ttttttattt ccagggatct
      961 ttattcatga ttaccccgat taatttttct ttaggtaaaa aatatagcgc ttcttctacc
     1021 tgtaattttg gagtaacact ggcttcaacc accattaaaa tgcgatctac cctcgtcgat
     1081 aatactttgc aatctgcata gcttctaata gcgcgtgaat ctaaaattac aaaactactg
     1141 ggaataaatt cttgaatttt ttgaaaaaag tcaaccattt ttgtgctagc aatgagttca
     1201 gaccgcaaat gtatttgttg gcctatagga acgacataac atttagcctg cgaagtctca
     1261 taaagtgcca tttcaagatt tatatcacct accagataat cggtaagccc tttgcattga
     1321 tttaaattta acaaagatgt aatggaattt gcattgccag aagtgtccac taagatggat
     1381 ttccaatctc tttccaatga catgcttacg gctaaattaa acgctgcagc gctttttcct
     1441 tcacctaccg atgcactggc tatcaataag gttttgcctc gcgaaggaaa gcgatggtga
     1501 tttaatattt cccattttat gccacggtat ttttctgcaa tactaccttg atcataagga
     1561 aactctaagt agccttctaa ttgaatttgt tttagatcaa taggtttccc taaacttaag
     1621 tcaggaataa ttaaattttt atgcgggtcc ttattgggct tggattttct ttgaggcaaa
     1681 tcatttatca tgtaggcatc actccttgcc tttgcatata gtaaaggagc atgaatcctc
     1741 cccatagtcc taatatacag attacataca gaagatttaa cttaagcgat atttttcgtt
     1801 taatatgatt tttttcttga ggaataacgg ctaacacagg taaatttagt gctttttcta
     1861 aagaaatttt atcataaaat gcaggcttcc attgcgccaa cacgaaagta gaagccactc
     1921 ctcctaacaa agaagctact aaaacaacta agttaaacaa attgcgcttc ggccaaacag
     1981 gtaaagaaga ggcatagggt ggctcaataa ttctgaacgt taaattttcc gaggtgccat
     2041 caatgtcttc aactagtttg gcggcatcgc gtttttctaa taatgcattg tactgtgcct
     2101 tattcatgct gtaatcacga tttaaattag aaagttttgt ttcggcatct attatttgat
     2161 ctaccttttc ttccatggat tg
//
LOCUS       DASTIB010000034         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_401367, whole genome shotgun sequence.
ACCESSION   DASTIB010000034 DASTIB010000000
VERSION     DASTIB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401367"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFP93_00540"
     CDS             complement(<1..692)
                     /locus_tag="VFP93_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9843125.1"
                     /translation="MNSYEEAYIVGAIRTPVTKAITGSFSQTRPDDLLAAVFKELLKK
                     IPSLDPVQIEDIIIGCAMPEGAQGMNVARIAALMGGIPDSVPAMTINRFCASGLQAIA
                     LAREKIVLGEADVIIAGGVESMSLIPMGGYHYRANPKWFEHDGPTSIAFTMGITAEIV
                     GKKWNISREKQDEFSLRSHQLAIDAQDNNYFDQEIVPITIQRESVDLHTREVIRKSII
                     IRSDEGPRNDYN"
     gene            complement(702..>2719)
                     /locus_tag="VFP93_00545"
     CDS             complement(702..>2719)
                     /locus_tag="VFP93_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9843126.1"
                     /translation="VLATNTSGLSIESLAQSLPWELKARFLGIHFFNPPRYMRLVEFI
                     PNTHTHKLLLEQLEEFFVVRLGKGVVYAKDTPNFIGNRIGVFSVLSILRHADNFRLAP
                     DKVDALTGLVIGRPKSATCRTMDIVGLDVMRHVVQTMQKDLKEDPWHSYFQLPAWVEE
                     LINRGSLGQKSCSGIYEKVSGNIQVFDASSKQYRPIHPKLNATIAEIVKLPWALKLKR
                     LYESAEDEAQFLWCCFRDLFHYSAYHLREIADSTRDVDLTMRWGYGWNHGPFEIWQQA
                     GVSWVKDTLNQEIMQGKTVNAQLPAWLSTIHDNGFYTDKGAFGPSASTYIPRSKQSFY
                     QRQLSHERFPGEAPLTGRTIFENDGVVMWTIDDKFAIVSFKSKKNSISHAVLEGIHES
                     LERAEKEYQALIIWQRQEPNFSVGANLQEILQAAKTRKFETINALIQDFQHVASKLKF
                     SEIPIVAAIEGLCLGGGTEFALHAYRRVAHFESYIGLPEAGIGLIPAGGGCKEMLLRA
                     NQRTLASQNLIALEKIFFHIAKSEISSNAKEARMMWYLDPADSIVMNAHEVLYEALRI
                     AQSVSGPNYQPPIPAKITVAGKEALANLQAILINLREGNFISDHDLRVGNLLAKVCCG
                     GVVDTNSVVDEKWLLDLERNVFMELVESELSLLRIEHILSTGKPLRN"
BASE COUNT          770 a          601 c          495 g          853 t
ORIGIN      
        1 atattataat cattgcgtgg tccttcgtcc gaacgtatga ttatgctttt tcttattact
       61 tcccgcgtat gtaaatctac actttctctt tgtattgtta tgggtacaat ttcttgatca
      121 aaataattgt tatcttgtgc atcgatagct aattgatggc ttctaagaga aaattcgtct
      181 tgtttttctc gagaaatatt ccattttttg ccaacgatct cggcggtaat tcccatagta
      241 aacgctatgc tggttggccc atcgtgttca aaccattttg ggttagctcg atagtgatag
      301 cctcccatcg gtattaaact catgctttca acaccacccg ctataataac atcggcttcc
      361 cccaatacga ttttttctcg tgctaatgct attgcttgta acccggatgc gcaaaatcga
      421 ttgatggtca tagcaggtac tgaatctggt attccaccca ttaatgctgc aatacgggcg
      481 acattcattc cttgcgcacc ttccggcatt gcgcatccga tgatgatatc ttctatttgc
      541 acgggatcga gtgagggtat tttttttaat aactctttga aaacagccgc aagtaaatca
      601 tccggtctcg tttgtgaaaa agaaccggta atggcttttg ttacaggcgt acgtattgca
      661 ccgacgatat aagcttcttc gtaggagttc atttttgatc cttaatttcg caggggtttt
      721 cctgtggata aaatatgttc aatgcgcaac agacttagct ctgattcaac cagttccata
      781 aatacattcc gttcgagatc aagcaaccat ttttcatcta caacagaatt ggtatctact
      841 acacctccac aacaaacttt cgcaagcaaa tttcctaccc taagatcgtg gtcagaaata
      901 aaatttcctt cgcgcaaatt aattaatata gcttgtaaat tcgctaaagc ctctttgcct
      961 gcaacagtaa tctttgcagg aattgggggt tgataattag gaccagaaac gctctgggca
     1021 attcgtaatg cctcatagag aacctcatgc gcgttcataa caatggaatc cgctggatct
     1081 aaataccaca tcattcgagc ttcttttgca ttggagctta tttcagattt tgcaatatga
     1141 aaaaatattt tttccaaagc aattaaattt tgtgaagcta aagtgcgttg atttgcgcgc
     1201 aacaacattt ctttacaacc tcctcctgcg ggaattaaac caattcctgc ttccggaaga
     1261 ccaatgtaag attcaaaatg cgctacccta cggtatgcat gtaacgcaaa ttcagtaccg
     1321 ccgcccaaac acaaaccttc tatagcagca acaataggta tttcggaaaa tttcagcttt
     1381 gacgcaacgt gctgaaaatc ttggataaga gcatttatcg tttcaaattt acgtgttttg
     1441 gctgcttgaa gtatctcttg taaatttgct ccgacactaa aattgggttc ttgacgctgc
     1501 caaataatca gtgcttgata ttctttctct gccctctcta gcgattcatg tataccctct
     1561 aaaactgcat gacttatcga atttttcttc gatttaaaac ttacgatggc aaatttgtca
     1621 tcgatcgtcc acataacgac accatcgttt tcaaaaatag ttctcccggt taaaggagct
     1681 tcgcctggaa atctttcgtg cgatagttgt ctttggtaaa aagattgttt tgatcgcggt
     1741 atataagtac tcgctgatgg accaaatgcc cctttgtctg tgtaaaaacc attatcatgg
     1801 attgtgctta accatgctgg taactgtgca tttacggttt tgccttgcat gatttcttga
     1861 ttgagcgtgt cttttaccca agatacaccg gcttgctgcc aaatctcaaa tggtccatga
     1921 ttccaaccat acccccaacg catagttaaa tctacgtctc ttgtcgaatc agcaatttct
     1981 cttagatggt aagcgctata gtggaataaa tctcgaaaac aacaccataa aaattgtgct
     2041 tcatcctctg cactttcata aagtcgctta agttttaatg cccacggtaa cttgactatt
     2101 tccgcaatag tagcattcaa cttgggatgg atcggacgat attgtttaga agaagcatca
     2161 aaaacttgaa tatttcctga gaccttttca taaataccac tacaggattt ctgccccaac
     2221 gaacctctat taataagttc ttctacccac gctggcaatt gaaaatacga atgccacggg
     2281 tcttctttta gatctttttg catggtttgc acgacatgac gcatcacatc gagccctaca
     2341 atatccatcg tgcgacaggt agcagattta ggtctgccaa ttactaatcc cgttagcgca
     2401 tctactttat cgggtgccaa acgaaaatta tccgcatgac gtagtatcga aagtaccgaa
     2461 aatacaccaa tgcgattacc tatgaaattg ggagtatctt tcgcatagac aactcctttg
     2521 cctaaacgaa ccacaaaaaa ttcttctaat tgttctagca gtaatttatg ggtgtgcgta
     2581 ttcggtataa actcaacaag tcgcatataa cgcgggggat taaaaaaatg tattcccaaa
     2641 aaacgggctt ttaactccca gggtaaagat tgcgccaagc tttcaatgga gagtcctgaa
     2701 gtattggtcg caagtacgg
//
LOCUS       DASTIB010000035         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_355133, whole genome shotgun sequence.
ACCESSION   DASTIB010000035 DASTIB010000000
VERSION     DASTIB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355133"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VFP93_00550"
     CDS             complement(<1..1946)
                     /locus_tag="VFP93_00550"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HET9843127.1"
                     /translation="MLKRAKEKQREAIEKAIQHIYRKMPNEEAALIEKFVQQYYVSAA
                     PDDLTRLSVIDLYGSILSHWNFLNQRNPNETKVRVYNPQFEQHGWQSTHSVLEVITDD
                     MPFLMDSVRMELNRRGLNLHLILHIGNMQVERDKNGRLKNVQFKKKKPDQGIVEAPLF
                     IEFDRQSEPAVLDEIKSSIESILSDVRLCVEDWPNMLQKMEEVILEVEQSKTSDNQKE
                     IQEYIDFLKWLEQDHFTFLGYCQLDIKENKEWVLVENSPLGLIKKGIEGYFDRLEDMP
                     FLAKQRMLSKEHIILGKTNQPSKIHRPANPDMVAIKCFDRLGEAIGQHCFIGLYTSEA
                     YNRSSQNIPLLNRKVRKILQISNFPPSSHDGKVIVNILETIPRDDLFQGTIDDLYRIA
                     IGILYLQERRNIRLFIRKDIFGHYFSCLVFVPRDRFNSGLRMTFSKILEEELQGKLLD
                     FNTRFSESILCRIHFIIQIDPKSDVDYSEHAIEERLKEAARTWDDSLKDALIDHFGEE
                     EGNRLIHKYARVFPPGYRASFLARTAVYDISHIENLNETKRLGMSFYRPLEEAEGRVR
                     LKLFHLGSSIALSDVIPMLENMGLRIIDERPHQITNNEGHTIWINDFGMFHESGADLN
                     VEAVKDVFQEAFYNIWYGFAESDG"
BASE COUNT          629 a          431 c          365 g          702 t
ORIGIN      
        1 aacccatcac tttcagcaaa gccataccaa atgttataaa atgcttcttg aaaaacatct
       61 tttacggctt caacatttaa atccgcgccc gattcatgaa acatcccaaa atcattgatc
      121 caaatcgtgt ggccttcgtt attggtaatt tggtgtggcc tttcatcgat aattcgaagc
      181 cccatatttt ctagcatggg aataacgtcg gataatgcaa tagagctacc cagatgaaat
      241 aattttaaac gtacccttcc ttctgcttct tcgagaggac ggtaaaaact catgccgagt
      301 cttttggttt catttaaatt ttcaatatgt gaaatatcat agacagctgt gcgcgctaga
      361 aaactggcgc gatagcccgg tggaaaaaca cgcgcgtatt tatggataag acgattaccc
      421 tcttcttctc caaaatgatc aataagggca tcttttaaac tgtcatccca cgttctagcc
      481 gcttctttta atctttcttc aatggcatgt tcgctgtaat ccacatccga tttgggatcg
      541 atttgaatga taaaatgtat acgacacaag atggactctg aaaaacgcgt attaaaatcc
      601 agtaatttcc cttgaagctc ttcttctaat attttagaaa atgtcatccg aagtccggaa
      661 ttaaagcgat cccttggcac aaaaactaaa caagaaaaat agtgtccaaa aatatctttt
      721 cgaataaata gtcgtatatt acgacgttct tgtaaatata aaatacctat cgctatacga
      781 tacaaatcat cgatcgtgcc ttgaaataaa tcatcacgtg gaatagtctc taagatattt
      841 acgatcactt taccgtcatg gctgctggga ggaaaattag aaatttgtaa aatttttctt
      901 acttttcgat taagtaaagg aatattttgt gaactccggt tataggcttc agaggtataa
      961 agtccaataa aacagtgctg ccctatagcc tcacctagtc gatcaaaaca tttaattgcc
     1021 accatatctg ggtttgcagg tctatggatt ttggaaggtt gattggtttt ccctaatata
     1081 atgtgttctt tcgaaagcat gcgctgtttt gctaaaaatg gcatatcttc caatctatca
     1141 aaataaccct caattccctt ttttattaac ccaagtggac tgttttccac taacacccat
     1201 tctttatttt ctttaatatc gagttggcaa tatcctaaaa atgtgaaatg gtcttgttct
     1261 aaccatttta aaaaatcgat atattcttgg atctcttttt ggttatctga cgttttactt
     1321 tgttcgacct caagaatgac ttcttccatt ttttgaagca tattcggcca atcttctaca
     1381 cataagcgca catcgctcaa tatagattca atggaacttt tgatttcatc taatactgct
     1441 ggttcacttt ggcgatcaaa ttcaataaac aatggcgctt ctacaatgcc ttgatcgggc
     1501 tttttctttt taaattgtac attttttaaa cgtccgtttt tatcgcgttc aacttgcatg
     1561 ttgccaatgt gcaatattaa gtgcaaatta agtccgcgcc gattcagttc catgcgaacc
     1621 gaatccatca ggaacggcat atcatccgta atgacttcca gaaccgaatg ggttgattgc
     1681 caaccatgtt gttcaaattg cgggttataa actcgcactt tggtttcatt ggggttcctt
     1741 tgattgagaa aattccaatg cgagagaatg gaaccataca ggtcaataac gcttaagcgc
     1801 gttaaatcgt cgggcgctgc agacacataa tattgttgta caaatttctc aattaaagcc
     1861 gcctcttcat ttggcatttt tcgataaata tgttgaatag ctttttcaat ggcttctctt
     1921 tgtttttctt ttgcgcgttt aagcattaag caccttggag ccccagtctg aattaattaa
     1981 tattacctga gtttgccaat caagacacta tcttgaggcg gtgcaatatc aaacattact
     2041 tgggaaacat ctagatttcg ttgccgaatg aagttggcaa ggttagttac ccaaatatca
     2101 ttgtcattta aacagggtat aagctct
//
LOCUS       DASTIB010000036         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_548584, whole genome shotgun sequence.
ACCESSION   DASTIB010000036 DASTIB010000000
VERSION     DASTIB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548584"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFP93_00555"
     CDS             complement(<1..642)
                     /locus_tag="VFP93_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9843128.1"
                     /translation="MKKIIVFIFSLFLCLHKAFATEMCFIAKENGKILKNEGDCKTPF
                     APESTFKITLSLIGFDSGILKDENNPSWTLPEGADPYINVCRGEHNPRTWMRDSCIWY
                     SRILTTKLGMEKFQDYVSKFSYGNKDLTGGLTNAWLSSSLKISPEEQTIFVQNLIDRK
                     FALSETSYEKTKHIMFIQELAGGWKLYGKTGNGRLIDKDGNKTQLQHGWFVGYI"
     gene            complement(650..1336)
                     /locus_tag="VFP93_00560"
     CDS             complement(650..1336)
                     /locus_tag="VFP93_00560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9843129.1"
                     /translation="MTFLYKIIDLTHTLNNNVPTWNGGCGFHHDLHLDYTDCEGEDKF
                     RVMKLRMHAGIGTHMDAPSHCIPGGECIDAIKVEELCMPCFVIDVSNKATEQYIVTLE
                     DILAFESKLGRISQGSCVMVKTGWEKNWHSPEKYHNNHVFPSVSIEAANLFLERDVRA
                     LGIDTLSADRPDNGFPVHQAFLGNGKLLIENVANLNKMPSCGSYIMAFPIKIKDGTEA
                     PIRLVGLIQN"
     gene            complement(1717..>2149)
                     /locus_tag="VFP93_00565"
     CDS             complement(1717..>2149)
                     /locus_tag="VFP93_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9843130.1"
                     /translation="NRYLQEQKSGERVVWLACVDEEIAGYVTLKWNSLYQPFQAKGIP
                     EIMDLNVLPSFRQKGVGNILLELAEQEASSKSKSVGIGVGLYAGKDGGYGAAQRLYVK
                     RGFIPDGNGVTYNYQPTIPGSNYILDDDLILWFTKTLTRQP"
BASE COUNT          650 a          438 c          348 g          713 t
ORIGIN      
        1 aatatacccc acaaaccaac catgctgcag ctgcgtttta ttgccgtctt tgtcgatcag
       61 tctaccattc cctgtttttc cataaagttt ccatccacca gcaagctctt ggataaacat
      121 aatgtgtttt gttttttcat agcttgtttc gctcaatgca aatttacgat cgataagatt
      181 ttgcacaaat atagtttgtt cttcgggcga aatttttaaa gaactggaga gccaggcatt
      241 tgttagcccg cctgtaagat ctttatttcc ataagaaaac ttcgatacat aatcctgaaa
      301 tttttccatg ccaagtttgg ttgttaaaat acgcgaatac catatacaac tgtctcgcat
      361 ccaagttcga ggattatgct ctcctctgca tacatttata taaggatctg cgccctcagg
      421 taacgtccaa gatggattat tttcatcttt caatattcca gaatcaaatc ctattaaact
      481 taaagtgatt ttaaaggtag attctggtgc aaatggcgtt ttacaatcgc cttcattttt
      541 caaaatcttg ccgttttctt tcgcgatgaa gcacatttct gttgcaaacg ctttgtgtaa
      601 acataaaaaa agagaaaata taaaaacaat aatttttttc atgattaacc taattttgaa
      661 taagtcccac taatcgtatg ggtgcttccg taccatcttt tattttgatt ggaaaagcca
      721 taatataact accacaggac ggcattttat ttaaatttgc aacgttttca attaataatt
      781 ttccatttcc taaaaatgct tgatgtacgg gaaaaccatt gtctgggcga tccgctgata
      841 aagtatcaat accaagagcc ctgacatcac gctcgaggaa aagattggcc gcttcaatgg
      901 aaacagaagg gaaaacatga ttattatgat atttttcagg agaatgccaa tttttctccc
      961 aaccagtttt aaccataacg catgatcctt gagatattct accaagcttg ctctcaaaag
     1021 ccaaaatatc ctccagtgtt acaatgtatt gctcagttgc tttattcgac acatctataa
     1081 caaaacatgg catgcataat tcttcaacct taatcgcatc aatgcactcc ccacccggaa
     1141 tacaatggct tggtgcatcc atatgtgtgc caatacccgc gtgcatgcgt agtttcataa
     1201 cgcgaaattt atcttcaccc tcgcaatccg tataatccag gtggagatca tgatgaaaac
     1261 cacaccctcc attccaagtg ggtacattat tattaagagt gtgcgtaagg tcgataattt
     1321 tatatagaaa tgtcatacat taaaatccaa taaatcttca atatcgataa tttctataaa
     1381 agtattgagc tgtggcaacc gaatgtgaca caccgcttaa atggaataat acgggtacgg
     1441 tcgctaagcc ttttggaaat ttaagattta aaattttttg ctttcacctc tttgattatg
     1501 tcactgctaa gttaaatatt ataatttggc ataaattcta attaaccaaa tgtgctcaaa
     1561 ataacttctt attcaatttt agcattaaag taccgtaatt tataagttag ttagtttgtt
     1621 gcttcagata tttattaaat ttaattgctt cacataaccc tgattgcatg atcgtgttta
     1681 atgcgtaagt tgtgttgttc aagcacctcc tttaaattac ggttgtcgtg ttaaggtttt
     1741 cgtaaaccat aaaatcaagt catcatcgag aatataatta cttcctggta tggtgggttg
     1801 ataattatag gtgacaccat taccatctgg tataaaccca cgctttacgt aaagcctttg
     1861 cgcagctcca taaccgccat ctttacccgc ataaagtcct acaccgattc caacgctttt
     1921 gcttttagat gatgcttctt gttcggccag ttctaaaagt atgttaccta ctcccttttg
     1981 gcgaaaagaa ggtagcacgt tgagatccat aatttctgga atacctttag cttgaaaagg
     2041 ttgatacaaa gaattccatt taagtgttac atatcctgcg atttcttcgt ctacacatgc
     2101 taaccacact acgcgctcac cggatttttg ttcttgtaag taacggtta
//
LOCUS       DASTIB010000037         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_429370, whole genome shotgun sequence.
ACCESSION   DASTIB010000037 DASTIB010000000
VERSION     DASTIB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429370"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFP93_00570"
     CDS             complement(<1..299)
                     /locus_tag="VFP93_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674875.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HET9843131.1"
                     /translation="MDWAILLQQLINGVVLGSIYGLIAIGYTMVYGIIGMINFAHGEV
                     YMISAYLTAIFLALFLSIGIQSPLLALCFTFILTLTITGLYGWAIERIAYRPLKGS"
     gene            complement(310..1395)
                     /locus_tag="VFP93_00575"
     CDS             complement(310..1395)
                     /locus_tag="VFP93_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9843132.1"
                     /translation="MCSTHVLGDTIKIGIAGPHTGPYAAFGDQLWRGASQAAADINKK
                     GGIHGDKIELVKADDACEPKQAVAVANRIVDFEDVEAVIGHFCSSSTIPASEVYADAG
                     VLMITPASTNPKVTDRGIKTVLRTCGRDDQQGKVAAEFIHQNLKASRVAVVHDKDTYG
                     KGLADAMRSRLEELGGKVSLYEGLTRGEKDFNALITKLKHVNADALYFGGLHTEAGPL
                     VRQLRNQGLKIPFISGDGIVSEEFVTSAGGPHVVKGVYMTFGADPRNFEQGKEVIAQF
                     RKTGYEPEGYTLYSYATLEIIAQAMTAVGSQDGEKLSAWLKANPVSTVIGVKEWDAKG
                     DLKHTDYVIYMWDDKGKYQEYYNPNRT"
     gene            complement(1450..2133)
                     /locus_tag="VFP93_00580"
     CDS             complement(1450..2133)
                     /locus_tag="VFP93_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HET9843133.1"
                     /translation="MHKIISFIILSFSSIFFTNALHADCYRSVSKTPEMISGLEKYVE
                     SGQYIKEIETKVLQAKDYLDGRLKNKGNQQFAIVLDVDETALSNYRTLKQHHFSTNRE
                     AFVASYLFGTQEAIGPVLGLFHYAKQRGIQIFFITDRPNTPEVIAHTIRNLKQVGFDG
                     WKEIYLLPLSHESVKIAEFKKQSRQKITDLGYTILLNIGDQSDDVVGGLAEISIQLPN
                     PFYTHTISG"
     gene            complement(2142..>2379)
                     /locus_tag="VFP93_00585"
     CDS             complement(2142..>2379)
                     /locus_tag="VFP93_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843134.1"
                     /translation="HWWYSLVIISTPFLLNLFLFLAIFISVVGIMTNFTATWDHAYFF
                     AFLLNTLLQVLFIPFIIGTSLCLLYDLKTKKIGK"
BASE COUNT          732 a          497 c          436 g          714 t
ORIGIN      
        1 gaacctttta aaggtctata agctatgcgc tcaatagccc aaccatagag ccctgtgatc
       61 gtaagggtga gaataaaagt aaaacataat gcaaggagtg gggattgtat gcctatcgat
      121 aggaaaagag ctaaaaaaat ggcagttaaa taggctgata tcatataaac ttcaccgtgg
      181 gcgaaattta tcatgccaat aatgccataa accatagtgt agcctatggc aattaatcca
      241 taaatagaac ctaaaaccac gccattaatt aattgttgta ataatattgc ccaatccatg
      301 gcatcccttt caagtccgat tcgggttgta atactcttgg tattttcctt tatcgtccca
      361 catatagatt acgtagtccg tgtgttttaa atcacctttc gcatcccatt ctttaacacc
      421 aatcacggta ctaacaggat tggctttaag ccatgccgat aatttttccc catcttggct
      481 acctacagct gtcatagctt gcgcaataat ttctaaggtg gcataagagt ataaggtata
      541 accttctggt tcataaccgg ttttgcggaa ttgcgctata acttccttgc cttgttcaaa
      601 atttctcgga tccgcaccaa aagtcatata aacccctttc actacatgcg ggccacctgc
      661 cgaggttaca aattcttcag aaacaatacc atcaccagaa ataaaaggga tttttaagcc
      721 ttggttacgc aattgacgaa ccaaaggtcc agcctcggta tgcaaaccac caaaatagag
      781 cgcgtcagca ttaacgtgtt ttaatttggt aattaaagca ttaaaatctt tttcgccccg
      841 ggttaatccc tcatataagg aaacttttcc ccctaattcc tccaagcgtg atcgcatagc
      901 atctgctaaa cccttgccat aggtatcttt atcatgcacc acagctactc tacttgcctt
      961 caggttttgg tgaataaatt ccgcagccac tttaccttgt tgatcatcgc gcccacatgt
     1021 gcgaagaaca gtttttatcc cacgatctgt cactttggga tttgtagagg caggagttat
     1081 cattaatacc cctgcgtctg cataaacttc tgaggctggt atggttgagg aagagcaaaa
     1141 atgcccaatt actgcttcta catcttcaaa gtcaacgatt cgattggcaa cagcaaccgc
     1201 ttgtttcggt tcacaagcat catccgcttt gacaagttct attttgtctc catgtatccc
     1261 acctttttta ttaatatctg ctgctgcttg agatgcgcct cgccacaatt gatcaccaaa
     1321 cgcagcataa ggtcctgtgt ggggaccggc tatgccaatt ttaatggtat cccctaatac
     1381 atgcgtgctg cacataatgc cagagattaa aacccataac cgaatagctt gtaacaaggc
     1441 aggctccttt taaccagata tggtgtgtgt ataaaaagga tttggcagtt gaatggaaat
     1501 ttccgcaaga cctccaacca catcatcgct ctgatcgccg atattaagca aaatagtata
     1561 accaagatcc gtaatttttt gacgtgattg ttttttaaat tctgctattt tcaccgactc
     1621 atggcttaaa ggaagaaggt aaatttcttt ccaaccgtcg aaaccgactt gttttagatt
     1681 gcggatagta tgcgcaatta cttctggggt attgggcctg tcggttataa agaaaatttg
     1741 aattcctctt tgtttagcat aatgaaataa tcctaacacg ggtccgattg cttcttgcgt
     1801 gccaaataga taagacgcta caaacgcctc gcggttcgta gaaaaatggt gttgctttaa
     1861 agtacgataa ttggaaagcg cagtttcatc cacgtccaaa actatggcga actgctgatt
     1921 tcctttgttt tttaagcgac cgtcgaggta atctttggct tgtaaaactt tggtttcgat
     1981 ttcttttatg tattgacccg actccacata tttttctagg cctgagatca tttcgggagt
     2041 tttggaaacc gagcgataac aatcggcatg taatgcgttt gtaaaaaaaa tactactaaa
     2101 acttaatata atgaaagaaa taattttatg cattgcgttt attatttacc tatttttttt
     2161 gttttgagat catataataa acacaaagag gttccaatga taaaaggtat aaataatact
     2221 tgtaataatg tattaagtaa gaaagcaaag aaatatgcat gatcccaggt cgcagtgaaa
     2281 ttggtcatga tacctactac cgaaataaaa atagctaaaa aaagaaaaag atttaataaa
     2341 aaaggagtac taataataac tagactatac caccaatgc
//
LOCUS       DASTIB010000038         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_488738, whole genome shotgun sequence.
ACCESSION   DASTIB010000038 DASTIB010000000
VERSION     DASTIB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488738"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFP93_00590"
     CDS             complement(<1..325)
                     /locus_tag="VFP93_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9843135.1"
                     /translation="MNIEIIQAKIEQKPILANLLELYAYDFTEFWDFDIGKNGFYGYE
                     WLSLYWKEANRFPYLIYVEDKIAGFVLVQKGSPISDDKDVYDMAEFFIMRKYKRKKIG
                     TIKRGK"
     gene            complement(322..693)
                     /locus_tag="VFP93_00595"
     CDS             complement(322..693)
                     /locus_tag="VFP93_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843136.1"
                     /translation="METIYSNKLKMKLSLVDEAFYNKALVYACAFWVIRVINCHVDEV
                     MKHDIICPSGPVDHDSRWEPEKNAFRPKILFWIEAFIFIAKKVNYLSALSRKASELSK
                     VQWPGTTFMDYYPVFMNKGAK"
     gene            2073..2249
                     /locus_tag="VFP93_00600"
     CDS             2073..2249
                     /locus_tag="VFP93_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843137.1"
                     /translation="MSEKQENLEENEQPWDPALPTFIPPIRISPTVDYDEYEREVAKL
                     QVLKNHKRGKGSRA"
     gene            2250..>2933
                     /locus_tag="VFP93_00605"
     CDS             2250..>2933
                     /locus_tag="VFP93_00605"
                     /inference="COORDINATES: protein motif:HMM:NF023673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HET9843138.1"
                     /translation="MKISAATATDVNGSLFANYAEFQKAAVYAHRHFLSKKYNNGSVD
                     FRWANQRSIIQFENGQSMDVPKQFILGIEEAQKLDKGKKNGEMVTLEEIVALYNKKRA
                     ADIKAGRVKALTRERVGDIDLDALNAVYRMKVVKIIHRPNHDTMHSIRAAAFIKAIHS
                     LISIGKPTPDQHTELDKSRVEKLQLMMLFSVLGREDETGFGDKKNANLYNSYRVVDAL
                     EFLKYCLDNW"
BASE COUNT          997 a          589 c          543 g          804 t
ORIGIN      
        1 ttttgcctct tttgatagtt cctatctttt tccgtttata ttttcgcata ataaaaaatt
       61 ccgccatgtc atatacgtct ttgtcatctg aaattggaga tcccttttga accaaaacaa
      121 accctgctat cttatcctca acatagatga ggtagggaaa tcgatttgcc tctttccaat
      181 ataaggacaa ccattcataa ccataaaacc catttttgcc aatatcaaaa tcccaaaatt
      241 ctgtgaagtc ataagcatat aactcaagta aattagccaa aattggtttc tgctcaatct
      301 tagcctggat aatttcaata ttcactttgc gcctttgttc ataaatacag gataataatc
      361 cataaaagtt gtgccaggcc actgaacttt cgaaagttcc gacgcttttc gagaaagtgc
      421 tgacaaatag ttaacctttt ttgcaataaa aataaatgcc tctatccaaa acaaaatttt
      481 ggggcgaaaa gcatttttct caggttccca ccgtgaatca tgatcaactg gtccactagg
      541 gcaaataatg tcatgtttca ttacttcatc gacatgacaa ttgataacgc gaataaccca
      601 aaaggcgcaa gcatacacta atgctttgtt gtaaaaagct tcatcgacta aggaaagttt
      661 catcttaagt ttattactat aaatggtttc catttgatga cgatatcttc agggatcact
      721 ttagcgcacc aacagctggc acgattttct gtaaagtaag ttgcaatttt tgcgaatatt
      781 tgctgatcga gtgtccctga acggggctag agtgtaacca gtaaaattac ttcatgcttt
      841 actcttaatt tcgaaattga actaacacgg agtgttaaag cactgcagat tttaagtcaa
      901 tgtctaacac agataaactc gaataacata aacaaactat atataaatca agggtatact
      961 tcaagtatat tttgaaagtg tttgagttaa tttgagtgga ttattgcgcc gccatacaca
     1021 cttttttatc ttttaacttc cagttgttcg gaatttccga ataactgccg cagatggtaa
     1081 aagctatgat actaatcact ataacttgtt aatcatttca gtttgatgta ggcatgaaca
     1141 acctcctgtg gcatgcctta acaatccaaa aaacactgct ttcttcacca tcctatacaa
     1201 atgtctaatt ttttgggact tataaataaa accctcatca cctctctgga ataaatgaaa
     1261 caccctattt tcctatttaa aacgcacaaa tgaacaacca atatgcggcc tttcccttcg
     1321 ccacttaggt attgtaaata atcactccaa ccattccata gctaaaggtt ttgacaaaat
     1381 cagcgattta agtgccatcc atcccgtata acacgcaaag aaatcagtga agttgcgcat
     1441 atatcagatt aaaaaatgaa tttttattga taataattgt caaatttcca agaacagtca
     1501 aatctgcagg gaaacttaac atattggatt ataaaaattt aaaaataccc agcgaggtgc
     1561 gtgagtatga caagggcacc ttaaaaaaat tgttaaatga aaggtgtttc gacaaagata
     1621 gttttagagt tattaatgaa tatttatttt tgcgaaagca aatctcagga aaaaaacctc
     1681 ctgaaactga gtgtacacgt gtgggccgct taatgcatta aggaaacttt aaacccaact
     1741 gaaaaatatt cggatgtgta ttttaaattg tggaatgaaa attctgtttt gcgccagaaa
     1801 ttgggtccaa aaaccaagga aacatcagga gacgatcgtt ggagatgaat caaaaaaaaa
     1861 taactctgca gttaactaag gagaacaaag catggctaaa aaacaatatc ccagttccaa
     1921 tcacgcaatt ggggagggaa aagcatgcgg atatcttgaa gtacaaagat tgctgatagc
     1981 ttaccaaatt ataccacagc cccgagatct cttcgttaac aagaaattca gagagatata
     2041 gcaagaacgc attaaaattt aagaggtttt taatgagcga aaaacaggaa aacctagaag
     2101 aaaacgaaca gccttgggat cccgcattgc ccaccttcat cccacccatt cggatcagtc
     2161 cgaccgtaga ctatgacgaa tacgaaagag aagtcgcaaa acttcaggta ttaaaaaacc
     2221 ataaaagagg taaagggagt cgcgcataaa tgaaaatcag tgctgcaact gctaccgatg
     2281 ttaatggcag cctttttgcg aactatgccg agtttcagaa ggcggcagtg tacgcgcacc
     2341 ggcatttttt gtctaagaaa tacaataatg gatccgttga ttttcgctgg gcaaaccaac
     2401 gcagtattat tcaattcgag aatggccaaa gtatggacgt tcctaagcaa ttcatcctcg
     2461 gaatcgagga ggcccaaaag ctagacaaag ggaaaaaaaa tggggagatg gtcacccttg
     2521 aagaaatcgt ggcactgtac aataagaaga gagctgctga cattaaagca ggcagggtaa
     2581 aggctctgac ccgagagaga gtgggagaca tcgacctcga tgcattgaac gcagtctatc
     2641 gtatgaaggt cgtcaaaatc attcatcgtc ccaatcatga taccatgcat tcgatcaggg
     2701 cggccgcctt tattaaagcg atccattccc ttataagcat aggcaagcca acacctgatc
     2761 agcataccga gttggataaa tcccgcgtcg aaaagctcca gctgatgatg cttttcagtg
     2821 tccttggccg cgaggacgaa acaggattcg gcgacaaaaa aaatgccaac ctctataaca
     2881 gctatcgggt tgtcgacgcg ttagaattct taaagtactg cctcgacaat tgg
//
LOCUS       DASTIB010000039         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_905928, whole genome shotgun sequence.
ACCESSION   DASTIB010000039 DASTIB010000000
VERSION     DASTIB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905928"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFP93_00610"
     CDS             <1..928
                     /locus_tag="VFP93_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843139.1"
                     /translation="PAPTGVEDVREPGTAAEDAAPSVPEQRDAEPETTTTPEGSVAPG
                     VEPSAPPLSEEPGAPSPTEPTDPETPAVSEERDESATPAVPEERKTEPSAPSLPDIDA
                     PTTEGGPNVPEEPRVEPSAPSTTEEPETSLPSTDEESTSVPESLRDREPIDVPQVPTE
                     DAPSIQEQIDQIPSESDRNLIPTPREEEAQPPEESSAPIQDIPRDITAPDAPPGAPSQ
                     DMPDRSEEVAPRFDSNVHPEPKSPEMSPPVEQVIPPQTQEPTPPLDSSSIPTPEPIAP
                     PMLEAPAAIPTPSEPQVIPPVDPAINPEEKKE"
     gene            1067..2248
                     /locus_tag="VFP93_00615"
     CDS             1067..2248
                     /locus_tag="VFP93_00615"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9843140.1"
                     /translation="MVHYDVLIIGAGAAGLMCAIEVGKRKRKVLVLEKANKPGKKILM
                     SGGGRCNFTNYHVHPNKYISKNPHFCKSALNRYTQWDFIELVQKHQIAYHEKKHGELF
                     CDDKSKDIVNLLLKEIQQAHVELELQTNIEHIQKLDNDKFLVVTAKYKVTTDSLVIAT
                     GGLSIPSLGSSPFGYKIAMQFGIDIVPVRAGLVPLTWKQEDKTKFECLSGIAIDAQVR
                     CEKTQFHENILFTHRGLSGPAILQISNFWQEHDELVMNLLPGKDLNALLHQQKAKAPL
                     QTIKHNLSNLLPARLVQTFLKQEDLMLTLSNASKKKLESIAHTFQTFRIKPNGTEGYR
                     TAEVTLGGVDCDAISSKTMECTKVKGLYFIGEVLDVTGWLGGYNFQWAWSSGWAAGQV
                     A"
     gene            2285..3067
                     /locus_tag="VFP93_00620"
     CDS             2285..3067
                     /locus_tag="VFP93_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9843141.1"
                     /translation="MSIFVFDIETIPDTQLGRRLLNIEANDRETADAMLLLQRQTQGS
                     DFPKHHLHRIVAISCVFRHRDSLKIWSLGEETSDEKTLIERFFSGITKFIPTLVSWNG
                     TGFDLPVLHYRALMHAIPSSVYWETGDFDSNFRWNNYLNRYHTRHIDLMDTLSAFQPR
                     AFAPLHEMALALGLPGKMGMDGREVWNAYQAGEIQRIRNYCEIDVINTYLIFLRFEFI
                     RGRLNTEEYLLEQQKLRDHLENEKHEHFQAFLKVWNETYRNE"
     gene            3060..>3543
                     /locus_tag="VFP93_00625"
     CDS             3060..>3543
                     /locus_tag="VFP93_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET9843142.1"
                     /translation="MSKRKKIPVGEYKAIITKLSHDGQGIAEIEGKKIFIAHALPQEE
                     VTFAYTYVKRQFAQGKAISVQQPHANRVEPRCEAFARCGGCSLQHMSTEAQIHLKENA
                     VRELFHSVAKVNPKTWLAPLVDASWGYRKKARLGVRLVLQKGGVLIGFREKFSNKIAV
                     I"
BASE COUNT         1104 a          738 c          767 g          934 t
ORIGIN      
        1 tccagcacca acaggagttg aggatgtaag agaacccggt actgctgcgg aagatgccgc
       61 gccttcagtg ccagaacaac gggatgcaga acctgaaact actacgacac ccgaagggtc
      121 ggttgcaccc ggagttgagc ctagtgcacc acctttatca gaagaacccg gagcaccatc
      181 accaaccgag ccaacagatc cagagacacc agcggtatct gaagagcgag atgaatcagc
      241 cacacctgcg gtacctgaag agcgcaagac ggaaccgagt gctccatctt taccagatat
      301 tgacgcacct actaccgaag ggggtcctaa tgtaccggaa gaacctagag tagaaccaag
      361 tgcaccttct acaacagaag aaccagaaac atctttacct agcacagatg aagagtctac
      421 atccgtacca gaatcattac gagatagaga gccaatcgat gtgccgcaag tacccacgga
      481 agatgcacct tcaattcagg aacaaataga ccaaatacct agcgagagcg atcgaaattt
      541 aatacctaca ccgcgagaag aagaggctca acctccagag gagtctagcg cacctataca
      601 agatatacct cgcgatataa ctgcccctga cgcgcctcct ggtgctccat cacaagatat
      661 gcctgacaga agtgaagaag tggctccacg ttttgactct aatgttcacc cagagcctaa
      721 gagtccggaa atgagtccac ctgtagaaca ggtgataccc ccgcaaacac aagaaccaac
      781 gccaccgtta gattcgagtt caattcctac accagaacct atagctccgc ctatgctcga
      841 agcacccgca gcgattccta cacctagtga gccgcaagta attccacccg tcgatcccgc
      901 aataaatcct gaagaaaaga aagaataaaa gcgagggggt gtgctatgca cacctttctt
      961 tctttatttt aagataactt tgcaactgac acaaaattct cttaaaattt ctgtgctgtt
     1021 aagaaattag ttattattgt agctttatca attgcgaggc gcttctgtgg ttcactacga
     1081 tgttttaata ataggagcag gcgctgcggg tctaatgtgt gcaattgaag taggcaaacg
     1141 caaacgcaag gttcttgtgc ttgaaaaagc aaataaacct ggaaaaaaaa ttcttatgtc
     1201 gggtggtggc cgttgtaatt tcacaaatta tcacgtacat cctaataagt acatttcaaa
     1261 aaatccccat ttctgcaaat cagcgcttaa tcgctatacc caatgggatt ttatagaact
     1321 cgtgcaaaaa catcagatcg cttatcatga aaaaaagcat ggtgaacttt tttgtgatga
     1381 caagtccaag gacatagtga atttattact gaaagaaatt cagcaggccc atgtagaact
     1441 agaattgcaa acgaacattg agcatattca aaaattagat aacgataaat ttttggttgt
     1501 caccgcaaaa tataaggtca ctacagactc tctggttata gctaccggtg gactttccat
     1561 tccttcgtta ggttcaagcc cttttggcta taaaatagct atgcaattcg gaatagatat
     1621 tgtacctgtg cgcgcgggat tggtaccact cacttggaag caagaggaca aaacaaaatt
     1681 tgaatgttta agtggaattg ctattgatgc gcaagtgcgg tgtgaaaaaa cacaatttca
     1741 tgaaaatatt ttatttacgc ataggggctt aagtggtcct gctattttgc aaatttccaa
     1801 tttttggcaa gaacacgatg agctggtgat gaatttactg cccggtaaag acttaaatgc
     1861 cttattgcac cagcaaaaag caaaagctcc tttacaaaca attaagcata atctcagtaa
     1921 tcttttgcct gcgcgtttgg tgcaaacttt tttaaagcaa gaggatctca tgttgacgct
     1981 tagtaatgca tcgaaaaaaa aattagaatc tattgcccac acatttcaaa cctttcgcat
     2041 aaagcctaac ggcaccgaag gttatcgaac cgcagaggta acgctcggag gtgttgattg
     2101 tgatgcgatt tcttcaaaaa ctatggaatg tacgaaagtt aaaggactct attttatagg
     2161 ggaagtttta gacgttaccg gatggttagg tgggtacaat tttcaatggg catggtcatc
     2221 tggttgggct gccggtcaag tagcctaaag acttattctt tcctattata gtcctggggg
     2281 aaatatgagt atttttgtat ttgatataga aaccatacct gatacacagt tagggcgtag
     2341 acttctgaat attgaagcga acgatcgaga aaccgcagac gcgatgttgt tgcttcagcg
     2401 tcaaacgcag ggatccgact ttcccaaaca tcatttacac cgcattgtgg caatatcttg
     2461 tgtatttcgt catcgtgata gtttaaaaat ttggtcttta ggcgaggaaa ccagtgatga
     2521 aaaaacgctt atcgaacgat ttttttcggg tattacaaag tttatcccaa ccttagtgtc
     2581 ttggaatggt acaggttttg atctacccgt attgcattat cgcgctttaa tgcacgccat
     2641 cccttcttcg gtttattggg aaacaggtga ctttgatagc aattttcgtt ggaacaatta
     2701 tttaaatcgt taccatacgc gtcatattga tcttatggac actttatcag cgttccaacc
     2761 acgtgctttt gcacccttgc atgaaatggc tttggcactc ggattacccg gtaaaatggg
     2821 aatggatggt agagaagttt ggaatgccta tcaagcagga gaaatccaac gtattagaaa
     2881 ttactgtgaa attgatgtca taaacacgta tttaattttt cttcgctttg aatttattcg
     2941 cggaaggctg aataccgagg aatacctttt agaacagcaa aaattacgcg atcatcttga
     3001 aaatgaaaaa cacgagcatt ttcaggcatt tttaaaagtt tggaacgaga cgtacagaaa
     3061 tgagtaagcg caaaaaaata cctgtgggcg aatataaagc tattattact aagttgtcgc
     3121 acgatggcca aggtattgcc gaaatagaag ggaagaaaat ttttatagcc catgcgttgc
     3181 cgcaagaaga agtaaccttt gcctatactt atgtaaaaag acaatttgcc caaggcaagg
     3241 ctatttctgt gcaacaaccg catgcgaatc gggttgaacc ccgatgtgaa gcttttgcgc
     3301 gttgtggtgg ttgttctctt cagcacatga gcacagaagc gcaaattcac ttgaaagaaa
     3361 atgcggtgcg agaattattc cacagtgttg ccaaagtaaa tccaaaaacc tggcttgctc
     3421 ctttggtaga tgcttcttgg ggataccgta aaaaagcaag gctaggtgtg cgcttggttt
     3481 tgcaaaaagg gggagttctc attggtttcc gcgaaaagtt ttcaaacaaa attgctgtaa
     3541 ttg
//
LOCUS       DASTIB010000040         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_712322, whole genome shotgun sequence.
ACCESSION   DASTIB010000040 DASTIB010000000
VERSION     DASTIB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712322"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /gene="prpD"
                     /locus_tag="VFP93_00630"
                     /gene_synonym="mmgE"
     CDS             <1..612
                     /gene="prpD"
                     /locus_tag="VFP93_00630"
                     /gene_synonym="mmgE"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="functions in propionate metabolism; involved in
                     isomerization of (2S,3S)-methylcitrate to
                     (2R,3S)-methylisocitrate; also encodes minor aconitase or
                     dehydratase activity; aconitase C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HET9843143.1"
                     /translation="SYVMENILFKISYPAEFHGQTAVECALKLHPKVKNNLENIKSMK
                     LITQESGFRIINKIGPLHNPADRDHCLQYMVAVALIDGKLTANSYSHEAAKNPLIDKL
                     REKMQVEESSTFSQDYLDPAKRSIANRIEIQYGDQILVEEIHYPIGHRFRREEGIPLL
                     LQKFNENLHAYYSSNKAQFLYEQCTHKSLFSLTVDKFIELWML"
     gene            complement(609..1430)
                     /locus_tag="VFP93_00635"
     CDS             complement(609..1430)
                     /locus_tag="VFP93_00635"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HET9843144.1"
                     /translation="MNRRTVFFVSDRTGITAETMGLSLISQFENIPFNQVTLPFIDTP
                     EKAHEAKERIILAKIADGHKPIVFGTIINEDVKLILGQTGAYFLDFFGTFIPSLETEL
                     HQKSSHTVGKIHGVKDVQAYNMRMNCVNFALTYDDGAKTSGYDLADIILIGVSRCGKT
                     PTSLYMAMQFGVQAANFPLTEEELTLAELPKVLRPHKTKLFGLTIDPERLHMIRSERR
                     PNCEYSDPKQCKKEIKLVESLYQREQIPYLSSTNMSIEEIATKIMAAKNLERRFR"
     gene            1506..>2933
                     /gene="ppsA"
                     /locus_tag="VFP93_00640"
     CDS             1506..>2933
                     /gene="ppsA"
                     /locus_tag="VFP93_00640"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020583434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HET9843145.1"
                     /translation="MAVSSYVIPFNQLGIHDIAQVGGKNASLGEMIRHLTNMGVKVPG
                     GFATTSDAFQEFLRQENLNQRIQTLLKKLNIEDLLGLQEIGFQIRSWIKDSPLQPELI
                     EAIAEAYKVLSPAAVAVRSSATAEDLPEASFAGQQETFLNVCGLENILHSVKAVFASL
                     YTDRAIAYRVHKGFKHEDVALSVGIQKMVRSDLASSGVMFTLDTESGFNKVVFITSSY
                     GLGELIVQGAVNPDEFYVYKPNLRNNNDPILRKTLGSKAQKMVFGTQQSIASIQTVNT
                     DIVDQNKFSLTDEDIIHLAHLATKIEDHYGCAMDIEWAKDGNSQELYIVQARPETIHR
                     HNARTLERYELLQQGKVVTTGRSIGQKIGQGKAKIINAITQIDEITSGDILVTDMTDP
                     DWEPIMKKVAAIITNRGGRTCHAAIIARELGIPAIVGTHDATLTIQNQQEITVSCAQG
                     DTGFVYEDKLPFKIHKIDLKQMPALP"
BASE COUNT          921 a          553 c          583 g          876 t
ORIGIN      
        1 agctatgtga tggagaatat tctatttaaa atttcttatc ctgcagaatt tcatggtcaa
       61 acagcagtag aatgtgcttt aaaactccat cctaaagtta aaaacaatct tgaaaatata
      121 aaatctatga agcttattac ccaagaatcg ggttttagaa tcatcaataa aataggccct
      181 ttgcacaatc cagcagacag ggatcattgt ttacagtata tggttgcagt tgccttaatc
      241 gatggaaaat taacggcgaa ttcctatagc catgaagcag caaaaaaccc gcttattgac
      301 aaattacgcg aaaaaatgca agttgaagaa tctagcacat tcagtcaaga ttacttagat
      361 cctgctaaac gatcgattgc gaatcgcatt gagattcaat acggtgatca aatattggtg
      421 gaagaaattc attatcccat aggacatcgt tttcgccgtg aagaaggcat tccattatta
      481 ttgcaaaaat ttaatgaaaa tttacatgct tattattcct ctaacaaagc acaattttta
      541 tatgaacaat gtactcataa atcactattt tctttaacgg tcgataaatt tattgagctg
      601 tggatgcttt agcgaaatct tctctcaagg ttttttgcag ccataatttt ggtagcaatt
      661 tcttcaatgg acatattggt tgagctcaaa taaggaattt gttcgcgttg atataaactt
      721 tccactaatt ttatttcttt tttacattgt ttaggatccg aatactcgca gttggggcgt
      781 cgttcgctgc gaatcatatg taatcgttcc ggatcaatgg tgagtccaaa tagctttgtt
      841 ttgtggggac gtaatacttt aggtaattct gcgagtgtta attcttcttc tgtaagggga
      901 aagttggctg cttggacgcc gaattgcata gccatgtaaa gactggtagg tgttttacca
      961 cagcgagata caccgattaa aataatatcg gctagatcgt agccggaagt ttttgcgcca
     1021 tcatcatagg ttaacgcaaa atttacgcaa ttcatgcgca tattatatgc ttgtacatct
     1081 tttacgccgt ggattttacc gaccgtgtgt gaagactttt ggtgcaattc tgtttctaat
     1141 gacggaataa aagttccaaa aaaatctaaa aaataagccc ccgtttgtcc taaaattaat
     1201 ttgacatcct cgttgataat agtcccaaat acgataggtt tatgtccatc tgcaattttt
     1261 gccaaaataa tccgttcttt tgcttcgtgc gctttttcgg gggtatcaat aaaaggtaat
     1321 gttacttgat taaagggaat attttcaaac tgggatatca aacttaatcc catggtttct
     1381 gcggtaattc cggtacggtc cgaaacaaaa aacacagttc gtcggttcat gaaaaaactc
     1441 cagactgatc tttgtacatt gtaatgttaa gatggcggaa attcatagcg aaaagcgagg
     1501 gtaccatggc agttagctcc tatgtaatac catttaatca attaggaata catgacattg
     1561 cacaggtagg tggtaaaaat gcatccttag gagaaatgat acgtcaccta actaacatgg
     1621 gggtcaaggt tccaggaggt tttgcaacca catccgacgc ttttcaggaa tttttgcgcc
     1681 aagaaaattt aaatcagcgc attcaaactt tgttaaaaaa attaaatatc gaggatttgt
     1741 tgggtcttca agaaataggg tttcaaatcc gttcttggat taaggattct cctttacaac
     1801 cggaactcat cgaggccatt gcagaagctt ataaagtcct atcgccagca gcggtagcgg
     1861 tgcgctcctc cgctaccgcc gaagatcttc ccgaagcctc ttttgcagga caacaagaaa
     1921 cttttctcaa tgtctgtggc ttggaaaata ttttacattc ggttaaagca gtttttgctt
     1981 ctttgtatac ggatcgtgca atagcctatc gtgtacacaa aggatttaaa catgaagacg
     2041 tagcgttatc ggtaggaatc cagaaaatgg tcagaagtga tttagcttca agtggtgtta
     2101 tgtttaccct tgataccgag tcaggcttca acaaggttgt cttcattact tcctcttatg
     2161 gattagggga actaattgtt caaggtgctg taaatccaga tgagttttat gtttataaac
     2221 ctaaccttcg gaataacaat gatcccattc tgcgcaaaac attaggatcc aaagcgcaaa
     2281 aaatggtatt tggtactcag cagagtattg cttccattca aaccgtaaac accgacatag
     2341 tagatcaaaa caaattttct ttgacggatg aagatatcat tcatcttgca catttagcaa
     2401 ccaaaattga agatcattat ggttgtgcca tggatataga atgggcaaaa gatggtaatt
     2461 cccaagaact ttacattgtg caagctcgac cggaaaccat tcatcgacat aatgctcgaa
     2521 ctttagagcg ctatgaatta ttgcagcaag gtaaagttgt gactacaggt cggagtattg
     2581 gtcaaaaaat agggcaagga aaagcaaaga tcataaacgc cattacacaa atcgatgaaa
     2641 ttacgtccgg tgatatttta gttaccgaca tgacggatcc cgattgggaa ccgattatga
     2701 aaaaggttgc agcaattatc accaatcgtg gtggtagaac ttgtcatgcc gccattattg
     2761 cacgagaact aggcattcct gcaatcgtag gtacgcacga tgcaacgctc accatccaaa
     2821 accagcagga gattacagta agctgcgctc aaggggatac cggtttcgtc tacgaggaca
     2881 aacttccttt taaaattcac aaaatcgatt tgaaacaaat gcctgcactt cct
//
LOCUS       DASTIB010000041         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_697364, whole genome shotgun sequence.
ACCESSION   DASTIB010000041 DASTIB010000000
VERSION     DASTIB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697364"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VFP93_00645"
     CDS             complement(<1..1445)
                     /locus_tag="VFP93_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9843146.1"
                     /translation="MAKDQQYAQPKRRSLIPLNLWDLIIIILFFGLMACIVWGAQQMS
                     GPYQLGEQIPIRTNPIMLPSYALYTVIRMAIALCFSIFVTFTMGTLAAKSKRAERIIL
                     PFIDIMQSVPILGFLSISLLAFIYLFPGSRLGPECAAILAIFTSQVWNMVLSFYQSVK
                     TLPKDLKETAAVYHLSGWQKFWRMEVPFAMPSLLWNTMASLSAGWFFVVASEAISVAN
                     QHITLPGIGSYIMLAQEQANYSALFYVIITMLIVILLYDQLIFRPLLVWSEKFKAEPS
                     EEVESAGSWFLDLLQRAHISKLITDVLYKMRELLITPKRAPKVHTPWSPKVYTVVSWT
                     AVTFWNTLIGLGLIGSVYLLVRYIYQDVTWQEVVHVFYLGFITTVKVTILIILSSLIW
                     VPVGVWIGLKPKLASFIQPIVQFIAAFPANLIYPILFMLILTYNLNVDIWTSPLMILG
                     TQWYILFNVIAGTLNIPLELRWAAKNYQVRG"
     gene            1609..>2211
                     /locus_tag="VFP93_00650"
     CDS             1609..>2211
                     /locus_tag="VFP93_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9843147.1"
                     /translation="MIKIHPPFIAPLASIMTLVLGAGFYTTLTTLDLTERDVDKWIIG
                     CISSSYFSGLLISAFRSQHFILRVGHIRAFAAFATLMCVTSLFQGLFFSPTLWVVLRF
                     LSGYALAGLAVVVESWLMDASDKKDRGAILAVYMFGYYLAQSISQIFLTLEYKQEIEQ
                     YILIAIFAALSILPVCMTRFPAPMPAYPNILSFQYLFKRAP"
BASE COUNT          717 a          425 c          420 g          649 t
ORIGIN      
        1 caaccgcgca cttgataatt tttggcggcc caacgcaatt caagagggat atttaacgtg
       61 ccagcaatga cattaaataa aatataccat tgtgtaccca aaatcataag aggagaggtc
      121 caaatatcta catttaaatt gtaagttaaa attaacataa ataagatagg gtaaattaaa
      181 ttcgctggaa aagccgcaat aaattgcaca ataggttgaa taaaagacgc aagtttaggt
      241 tttaaaccta tccaaacccc cacgggaacc caaatcaaag aacttaaaat gataagtatt
      301 gttactttta ctgtggtaat aaaacccaaa taaaaaacat gtacgacttc ttgccatgtt
      361 acatcttgat aaatgtagcg caccaataaa tacacagagc ctattaatcc taaaccgata
      421 agggtgttcc aaaaagtcac ggctgtccaa gaaaccacag tgtagacttt aggcgaccaa
      481 ggcgtgtgca ctttgggggc tcttttaggc gttataagta gttcacgcat tttgtagagt
      541 acatcggtta ttagtttgct aatatgcgcg cgctgtaaca gatctaaaaa ccaagaacct
      601 gccgattcga cttcttcact gggttcggct ttaaattttt ctgaccaaac taaaagtggg
      661 cggaaaatca gctgatcata cagcaaaatt acaataagca tggtgatgat aacgtaaaat
      721 aaagcgctat aattggcttg ctcttgggcc aacataatgt aagaacctat gccaggcagg
      781 gtaatgtgtt ggtttgcgac cgaaattgcc tcggaagcta ccacaaaaaa ccaacccgcc
      841 gagagagaag ccatggtgtt ccataataaa ctgggcatag cgaagggtac ttccatgcgc
      901 caaaattttt gccaaccact aagatgatac accgccgctg tttcttttaa atccttaggc
      961 agcgttttta ccgattgata aaaacttaac accatgttcc atacttgcga agtaaatatt
     1021 gcaagaatcg ctgcacattc gggacctaat cgacttccgg gaaaaagata aataaaagct
     1081 aaaagactaa tagataaaaa acctaaaata ggaacggatt gcatgatatc gataaagggc
     1141 aaaataatcc gctcggcgcg cttacttttt gcagctaatg tacccatggt gaaagtcaca
     1201 aaaatagaga agcataaagc gatcgccata cgtattaccg tatacaatgc gtagcttggc
     1261 aacataatag gatttgtacg tataggaatt tgctcaccga gctgataagg gccactcatt
     1321 tgctgagccc cccacactat gcaagccatc aaaccaaaaa acaatataat gatgattaaa
     1381 tcccataaat ttaatgggat taaggatctt ctttttggtt gtgcatactg ttgatctttt
     1441 gccatagcat caaataataa aaagtaatta aattaacaaa caattaagtt tgaagccaat
     1501 cattctagct tattataact gtacttttat tacagtcgac acttttgcat aaatcttggt
     1561 acgctagcct gtgggtattg ttaaacgaag tcagaaattg gagttgtgat gataaaaatc
     1621 catcctccgt tcattgctcc attagcaagt ataatgactt tggttttagg ggcggggttt
     1681 tacactacgc taactacttt agacttaacc gaacgtgatg tagacaaatg gatcatcgga
     1741 tgtatttcta gtagttactt cagcggatta ctcataagtg cctttcgctc tcaacatttt
     1801 atcttaagag ttggtcatat tcgtgctttt gcagcgtttg ctactttaat gtgcgtcacc
     1861 agcttatttc aaggtttatt cttttcacca acgctttggg ttgtgttacg ttttttaagt
     1921 ggctatgctt tggcaggtct tgcagtcgta gtagaaagtt ggctaatgga tgcaagcgat
     1981 aaaaaagata gaggtgctat tcttgctgtt tatatgtttg gttattattt agcccaaagt
     2041 attagtcaaa tatttttaac actagaatac aagcaagaaa tcgaacaata tattttaatt
     2101 gcgatttttg cagccctttc gatcctaccg gtttgtatga cgcgttttcc ggcgcccatg
     2161 cctgcttatc ccaatatatt atcttttcaa tatttattta aacgcgctcc a
//
LOCUS       DASTIB010000042         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_56648, whole genome shotgun sequence.
ACCESSION   DASTIB010000042 DASTIB010000000
VERSION     DASTIB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56648"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFP93_00655"
     CDS             <1..942
                     /locus_tag="VFP93_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET9843148.1"
                     /translation="WDYCHQDVLVSQFIEGIPVSHIDSLKQANVNLKLLAERGVEIFF
                     TQVFRDCFFHADMHAGNIFVDAREPDNPKYIAVDFGIMGSLNPTDQKYLATNFLAFFK
                     RDYRKVAQSHIDSGWVPSHTRVDEFEASIRTVCEPMFEKPLKDISFGRSLLRLFQTAR
                     KFDMEIQPQLLLLQKTLLSVEGLGKQLYPELDLWHTAKPFLERWMKTHMGPKALIKHM
                     REKLPFWFYKMPEIPEVMHNLAQHWHHEGLRKEKRPVPPKSVRNIILTTCSSILLLGG
                     IFTLGYSHRDWILQYPTWVGGALCFLGLVLYVLPVRQ"
     gene            1015..1347
                     /locus_tag="VFP93_00660"
     CDS             1015..1347
                     /locus_tag="VFP93_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9843149.1"
                     /translation="MDCLFCKLDKGEIPTEKLYEDDKIFVIKDIHPKADVHLLIIPHL
                     HIESLLDVNEAHENLLGYMISSLKNFAKTQGISGFKTLINTGKTGGQEIFHLHIHLLG
                     GKKIPKIA"
     gene            1357..1524
                     /gene="tatA"
                     /locus_tag="VFP93_00665"
     CDS             1357..1524
                     /gene="tatA"
                     /locus_tag="VFP93_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9843150.1"
                     /translation="MGLSGISPGSLLLIFLIVLILFGTKKLSTIGEDLGRAIQGFRKG
                     LKDEKTNPNGT"
     gene            1525..1719
                     /locus_tag="VFP93_00670"
     CDS             1525..1719
                     /locus_tag="VFP93_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843151.1"
                     /translation="MFEIGWGELCVVGLLMLFLIKPEHIPVFAKIMGRKLLQLKSSLD
                     RFKAELKAEFQDNTNNVKDE"
     gene            1712..2464
                     /gene="tatC"
                     /locus_tag="VFP93_00675"
     CDS             1712..2464
                     /gene="tatC"
                     /locus_tag="VFP93_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9843152.1"
                     /translation="MNKSAQFNLISHFLEIRKRVLYCLITFSVVALPCIVFAKELFET
                     TTAWFFFPLPRDYTFIATEVTSTFVIPLKLALFATLFCVAPFILYQGWAFITPGLYAH
                     EKRHLRISFIASVGLFYVGAAFAYFLVCPLALQFFIGQAPSNVHVMTDLSHYLNFIIK
                     MILSFGIGFQVPIIIFLSLQAGICSIQSLKNKRRYIIVLAFVLGMLLTPPDVISQVLL
                     ALPMIALFELGIISYTLFGHSTCTKLRNTNAP"
     gene            complement(2442..>2667)
                     /locus_tag="VFP93_00680"
     CDS             complement(2442..>2667)
                     /locus_tag="VFP93_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9843153.1"
                     /translation="DQILKNAFKLESIPGIIVHGRYDMICPLDNAYQLQQLWNKAELH
                     VVRDAGHSSMEPGITDALINATQIMAHLYS"
BASE COUNT          775 a          492 c          527 g          873 t
ORIGIN      
        1 tgggactatt gtcatcaaga tgttttagtt tcccaattta tcgaaggcat tccagtatcg
       61 catattgatt cgttaaaaca agcaaacgtt aatttaaaac tgctagcaga gcgtggggta
      121 gaaatatttt ttacccaagt atttcgcgat tgcttctttc atgccgatat gcatgccggt
      181 aacatttttg tggatgcgcg tgaacccgat aatcccaaat atatagcggt ggattttggg
      241 atcatgggta gcttaaaccc caccgatcaa aaatatttag caaccaattt tttggcgttt
      301 tttaaaagag attatcgaaa agtcgcccaa tcgcatatcg attcgggttg ggtaccatcc
      361 catacacgcg tcgatgaatt cgaagccagt atccgcaccg tttgtgaacc catgttcgaa
      421 aagccgttaa aagacatttc ttttgggcga tcgctactgc gcttatttca aaccgcgcgt
      481 aaattcgata tggaaattca accgcagtta ttattattgc aaaaaacgtt gttgagtgtc
      541 gaaggattag gcaaacagtt atatccagaa ttggatttgt ggcacaccgc caaacccttt
      601 ttagaacgct ggatgaaaac gcatatgggt cctaaggctt taataaaaca catgcgtgag
      661 aaattgcctt tttggtttta taaaatgccc gagatacccg aggttatgca caatttagcg
      721 caacattggc atcacgaagg tttacgcaaa gaaaaacgtc ccgttccacc taaatcagtt
      781 agaaacatta ttcttactac ctgttcaagc attttattgc tcggaggcat ttttacctta
      841 ggttactctc atagggattg gattttacaa tatcctactt gggtaggtgg cgcattatgc
      901 ttcctgggat tagttttata tgtgctacct gtacgacagt aagttttgcc tttttttaaa
      961 cttttgcgat agcatacagt caagaaaaat actaagagcc gaggaataag ttagatggat
     1021 tgtttgtttt gtaaattgga taaaggggaa atacctacgg aaaagcttta tgaagatgat
     1081 aaaatttttg ttatcaaaga tattcatcct aaggccgatg tccatttact gattattcct
     1141 catctacaca tagaaagttt actcgatgtt aatgaagcgc atgaaaatct attgggctac
     1201 atgattagtt ccttaaagaa ttttgcaaaa acccaaggta tttccggttt taaaacatta
     1261 attaatacgg gcaaaaccgg aggacaagaa atatttcact tacatattca tttactaggc
     1321 ggaaagaaga ttcccaaaat agcttaagga gtttttatgg ggttaagtgg cattagtcct
     1381 ggttcattgt tgttgatttt tctcattgtg cttatacttt ttggtacgaa aaaattaagc
     1441 accatcggtg aagatttagg tcgtgccata caaggttttc gcaaagggct gaaagacgaa
     1501 aaaacaaacc cgaatgggac ataaatgttt gaaataggct ggggcgaact ttgtgtggta
     1561 ggtttattga tgcttttttt aatcaaaccc gaacacatac ctgtttttgc caagattatg
     1621 ggtcgtaaac ttttacagtt aaaatcgagc ttggatcgtt tcaaagccga gctgaaggca
     1681 gaattccaag ataatacgaa taatgtaaaa gatgaataaa agcgcgcaat tcaatttgat
     1741 ctcgcatttt cttgagatcc gcaaacgcgt tttatactgt ttaataacct tcagtgtagt
     1801 agcgcttccc tgcatcgttt ttgcaaaaga gctttttgaa acgactaccg cttggttttt
     1861 ttttccttta ccccgcgatt atacttttat cgcaacagaa gtaacttcaa catttgtcat
     1921 accgctcaaa ttagcgctgt ttgctactct attttgcgta gcacccttta ttttatacca
     1981 aggatgggct ttcataaccc cggggttata tgcacatgaa aaaagacatc ttcgcattag
     2041 ttttattgcg agtgttggat tattctatgt aggagcggct ttcgcttatt tccttgtttg
     2101 ccctttagca ctgcaatttt ttatcgggca agcgccgagc aatgtacacg ttatgacaga
     2161 tttaagtcat tatttaaatt ttattataaa gatgatttta agcttcggga ttggttttca
     2221 agtgcctatt atcattttct taagtttgca agccggtatt tgtagtattc aatctttgaa
     2281 aaataaacgt agatatatta ttgttctcgc ttttgttctc ggcatgctgc taactccacc
     2341 ggatgtaatt tcccaagtgt tgctagcact acctatgatt gcgttatttg aattaggaat
     2401 aatatcctat acgctttttg gtcactcgac gtgtacaaag cttaggaata caaatgcgcc
     2461 ataatttgag tcgcattgat tagtgcatcg gtaatgcctg gctccatgga agaatgccct
     2521 gcatcacgaa ccacatgtaa ttctgcttta ttccataatt gctgtaactg gtacgcatta
     2581 tccagcggac agatcatatc atatcgacca tgcacaatga tgccgggtat actttcgagt
     2641 ttgaatgcat tttttaagat ttgatca
//
LOCUS       DASTIB010000043         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_741806, whole genome shotgun sequence.
ACCESSION   DASTIB010000043 DASTIB010000000
VERSION     DASTIB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741806"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..454
                     /locus_tag="VFP93_00685"
     CDS             122..454
                     /locus_tag="VFP93_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843154.1"
                     /translation="MNCIHHSTSVAVCQCVICHKGLCVDCFHELESLCVCKVGTCDKE
                     ALVYKNLLNMHKKHTFIPYLYLILGLLFILLGSWNIVEHHYSVFDIAMVILGGLFALQ
                     GLPAHKTK"
     gene            complement(461..841)
                     /locus_tag="VFP93_00690"
     CDS             complement(461..841)
                     /locus_tag="VFP93_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HET9843155.1"
                     /translation="MWEYFSQEELQCRCGCRQMHMKNSFMVKILSLRKLLQFPFKVTS
                     AYRCPSYNQYVSTSGLQGPHTTGRAIDIAVYGEKAFQLVTSAGICGLSGIGIKQSGSF
                     SKRFIHLDDLQLEDNFPRPNVWNY"
     gene            complement(1026..1277)
                     /locus_tag="VFP93_00695"
     CDS             complement(1026..1277)
                     /locus_tag="VFP93_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843156.1"
                     /translation="MKKISIAIAMATSFLLAGCAYDGHYYNQHRDHKVKCLLKECPNK
                     VKCVFKQCPRAKYYKPYAHQCRQCPMLSNYHHGGYHHYK"
     gene            1451..1798
                     /locus_tag="VFP93_00700"
     CDS             1451..1798
                     /locus_tag="VFP93_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HET9843157.1"
                     /translation="MQPAIEKHSNALSKCIKQCWDCRNECQKTLYNYCLNVGHVNEEH
                     IKIMTDCIQICQTAADFMTRHSNLHHEICKACAIICDNCAQSCRKIEDEQMESCAHFC
                     EQCAQSCQAMAKG"
     gene            complement(1814..2374)
                     /locus_tag="VFP93_00705"
     CDS             complement(1814..2374)
                     /locus_tag="VFP93_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9843158.1"
                     /translation="MRIAVSGTHCMGKSTLIQDFIELYPHYRCEIEAYYKLQEVKTME
                     VAQTPTFESLVEQLDFSIHQIHQNAQESLVIFDRCPIDYIAYAMLEAAYDEIDIHQTE
                     IADRFPEVKEALNLLDLIIFIPMHRAYPIAYHEENPEYRKEADHCFKRIYRDDVCDIF
                     PKFNHPRIVEVFGDRKKRIQTLKLYL"
     gene            complement(2512..>2755)
                     /locus_tag="VFP93_00710"
     CDS             complement(2512..>2755)
                     /locus_tag="VFP93_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843159.1"
                     /translation="MAFTYSYDSSLGQKKGLPSQTDEPLLQELADPKKTYMNAIVTLR
                     KKLRLQREVHEKDLKIAQQAKEIEELKQKLLNSTPR"
BASE COUNT          871 a          487 c          472 g          925 t
ORIGIN      
        1 gtcctaaagc acttacattt tacaaaaaac caacaataga tgaagatgct atgctgtccg
       61 atataaaact ttgacttagt ggccaaacat ttttaagctt taatttttct gcgatccatt
      121 tatgaactgt attcatcatt caacttcagt tgctgtttgc cagtgcgtta tatgtcataa
      181 aggattgtgt gtagattgct ttcatgaact cgaatcttta tgtgtgtgca aagtaggtac
      241 ctgcgacaaa gaagcattgg tatacaaaaa tcttttaaat atgcataaaa agcatacatt
      301 tattccgtat ctttatctta tattgggctt actatttatc ttgttagggt cttggaacat
      361 cgtagaacat cattattctg tttttgatat tgcgatggtt atcctaggtg gactttttgc
      421 acttcaaggc cttcctgccc ataagacaaa ataacctaaa tcaataattc caaacgtttg
      481 gcctggggaa attatcttct aattgtaaat catcaagatg aataaatctt ttagagaagc
      541 ttccactttg tttaattcca attccactta aaccgcaaat accggcactg gtaactaatt
      601 gaaaagcttt ttctccataa accgcaatat cgatggctct gccggttgta tgaggtcctt
      661 gcaatcctga ggtactgacg tattgattgt aagacggaca tcggtaggcg gaggtaactt
      721 taaagggaaa ttgtaaaagc tttcgcaacg ataaaatttt taccataaag ctatttttca
      781 tgtgcatttg gcgacaacca cacctgcact gcaattcttc ttgcgaaaaa tattcccaca
      841 tttttttcta tctgaccttc gcgaatgata aagataccag aattccttta agtagcaaat
      901 agatatgcaa atttggtaaa taagataaat gaatccaaaa tgcgtattca aatgaatttg
      961 ccccgttctt caacagaacg aggcaaactc taaaaatatt attgcaatta tgttcctatt
     1021 gacaactatt tataatgatg ataaccacca tgatggtaat tgctcaacat tgggcattgt
     1081 ctgcattgat gtgcataagg tttatagtat tttgctctag gacattgttt aaaaacacat
     1141 tttactttgt taggacattc ttttaacaaa catttcactt tatgatcgcg atgttgatta
     1201 tagtagtgac catcataggc acaaccagcc aatagaaaag aagttgccat tgcaatcgca
     1261 attgatattt ttttcataaa atacacctca aaaatgcaat ttagtaacaa aatacatcct
     1321 agcatttctt tttatttttt gttaaaaggt tatatttttc ttcagcttta atgcaatttt
     1381 tctaataaat tcattttttg ttctatattg aatctcataa ttaccctagt taaccgcaga
     1441 aggattgtgt atgcagccag ccattgaaaa gcatagtaat gccctaagca agtgtataaa
     1501 gcaatgttgg gattgtagaa atgaatgcca aaaaacactt tacaattatt gtttaaacgt
     1561 aggtcatgtg aatgaagaac acataaaaat catgacagat tgtatacaaa tttgtcaaac
     1621 agctgcggat tttatgacgc gacactcaaa cctacatcac gaaatttgca aagcgtgcgc
     1681 tattatttgt gataattgcg cgcaatcatg tcgtaagata gaagatgagc aaatggaatc
     1741 ctgtgctcat ttttgtgaac aatgcgcgca atcgtgtcaa gcaatggcaa aaggataaac
     1801 atttataatc aaattataaa taaagcttta acgtctgaat gcgctttttt ctatcgccaa
     1861 acacttctac aatgcgggga tggttaaatt taggaaaaat atcacaaaca tcatctctgt
     1921 aaattctttt aaagcagtga tccgcttcct ttctatattc tggattttct tcatggtatg
     1981 caatgggata agcccggtgc attgggataa aaataatgag atctaacaaa ttcagcgctt
     2041 cctttacctc aggaaatcta tctgctattt cagtttggtg aatgtctatt tcatcatatg
     2101 ctgcttctaa catggcatag gcaatataat cgataggaca cctatcaaat atcacaagcg
     2161 attcttgcgc gttttgatgg atttggtgga tactaaagtc taattgttcg actaggctct
     2221 caaatgttgg agtttgcgct acttccatgg tttttacttc ttgtagctta taataagctt
     2281 cgatttcgca cctataatgg ggataaagtt caataaaatc ttgaattaac gtagacttac
     2341 ccatgcaatg agtaccagaa actgcaatac gcatacttat acactcaaat ctaataaaaa
     2401 atttttgtaa taatactctg tcacttacag ctggacaagt tctacctaga gacctagcaa
     2461 aaatagcttc gcagtgaaac aaagacctcc gtaagaatcg gaggtaggta gttatcttgg
     2521 tgttgaattt aacaattttt gttttaactc ttcaatttct ttagcttgtt gtgcaatttt
     2581 taaatccttt tcatgcactt ctctttggag ccttagtttt ttacgtaagg ttactatagc
     2641 attcatgtaa gtttttttgg ggtcggctaa ttcttgcagt aaaggttcat ctgtttgcga
     2701 gggtaacccc ttcttttgac caagtgagga gtcataagaa taagtaaagg ccatg
//
LOCUS       DASTIB010000044         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_607743, whole genome shotgun sequence.
ACCESSION   DASTIB010000044 DASTIB010000000
VERSION     DASTIB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607743"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFP93_00715"
     CDS             complement(<1..347)
                     /locus_tag="VFP93_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843160.1"
                     /translation="MLKPIDLLVGLKILTSKQDWTQMGIAVELCLSSSQVNSAIKQLL
                     EANLFTMRQGKAYPIYAALKEFILYGVSYCFPAKTGELTVGVPTAYAAEPLSHEISVG
                     KDPIPIWPYAQGK"
     gene            complement(483..782)
                     /locus_tag="VFP93_00720"
     CDS             complement(483..782)
                     /locus_tag="VFP93_00720"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET9843161.1"
                     /translation="MCKSITFDQGCEFADHKFLEQHMKCSVYYCQTHSFWQKASNENM
                     NGRLRWHLPRETNISQKELEELALKMNRCPRKCLGYKTPQELFIQQHKNDCRTWS"
     gene            complement(713..1396)
                     /locus_tag="VFP93_00725"
     CDS             complement(713..1396)
                     /locus_tag="VFP93_00725"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET9843162.1"
                     /translation="MGFSKSEIARGLSKHRSSIYRELKRSTEPNGYFPKVAQIKMLER
                     AKQKRLSKLQTNGVLRDHIVRSLKKGWGPEQISGRMKYQKLTFYACHESIYQFVYRAK
                     SKELYHCLAYKQPKRKKRYSRHKQQCRYGDFRLITERPKEIGKRERFGHWEGDTIQFK
                     GIKEKVVTTLVERKSRLVFLIKNERKYSRGVMKKIKKNLNRCLARCVKASPLIKAVNS
                     LITSSLSST"
     gene            complement(1935..2123)
                     /locus_tag="VFP93_00730"
     CDS             complement(1935..2123)
                     /locus_tag="VFP93_00730"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET9843163.1"
                     /translation="MLLQELRKARQLSQKNIAEVLSTEKANISRLEQRTDMYISTLRS
                     YIEAIGSQLDIIASFLDG"
     gene            complement(2218..2376)
                     /locus_tag="VFP93_00735"
     CDS             complement(2218..2376)
                     /locus_tag="VFP93_00735"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET9843164.1"
                     /translation="MDPRILYAFDSKRVGILLIGGDKTGNNRWYEEYVLIADDLYNEH
                     IEALKKGG"
     gene            complement(3079..>3300)
                     /locus_tag="VFP93_00740"
     CDS             complement(3079..>3300)
                     /locus_tag="VFP93_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843165.1"
                     /translation="IEEMLPNIKLLDMFCDFLVSSGFQNHNISEKKLQASNLDEISND
                     AKYIKCFKNNMKLQKDINKYFGSTSTKRA"
BASE COUNT          957 a          652 c          570 g         1121 t
ORIGIN      
        1 ctcttacctt gcgcataagg ccaaatcggg atgggatcct tcccaacaga aatttcatgg
       61 cttaaaggtt cagcggcata ggctgttggt actcctacag ttaactcccc cgttttcgct
      121 ggaaagcaat aagatacgcc ataaagaata aattctttca atgctgcata aatgggatag
      181 gctttaccct ggcgcatggt aaataaattc gcttctaaca gttgtttaat cgcagaattc
      241 acctgtgaac tagatagaca taattccact gcaattccca tttgggtcca gtcctgcttt
      301 gacgttagga ttttaagccc tactaaaagg tcaattggct ttaacatagt gtcctcctac
      361 ttagtaggat agcacattct gtattctaaa atctagaata tagaataatg attgggtgta
      421 tactcaccca gtagcccttt acattccgtt tgggggacta cgaatttttc gcgaggcgca
      481 ttctaagacc aagtgcgaca atcattttta tgctgctgta taaatagctc ttgaggcgtt
      541 ttataaccca agcatttcct cgggcatcgg ttcattttta atgctaactc ctcaagctct
      601 ttttggctaa tattggtttc cctaggcaag tgccatctta agcgaccatt catattttca
      661 ttgcttgctt tttgccaaaa agaatgtgtt tggcagtagt aaacactaca cttcatgtgc
      721 tgctcaagga acttgtgatc agcgaattca cagccttgat caaaggtgat gcttttacac
      781 atcttgctag gcaacgattc aaattctttt ttattttttt catcacaccg cgcgaatact
      841 tgcgctcatt cttaattaaa aacacaagcc gagattttct ttctactaaa gtagtaacca
      901 ctttttcttt aattccttta aattgaatgg tatcgccttc ccagtgaccg aaacgttctc
      961 ttttccctat ttctttaggt ctttctgtga ttaatcgaaa atctccatat ctacattgct
     1021 gcttgtgtct agaataacgc ttttttcttt taggttgttt ataagccagg caatgataaa
     1081 gttctttgct tttagcgcgg tatacaaact gataaatgct ttcatggcat gcataaaagg
     1141 ttagcttttg atatttcatt cgtcctgata tttgttctgg accccagcct ttctttaaac
     1201 ttctgacgat atgatcacgc aaaacaccat ttgtttgcaa tttgctaagc cgtttttgtt
     1261 tagcccgttc tagcattttt atttgtgcta cttttggaaa atagccattt ggttctgtac
     1321 tgcgtttgag ttctcggtaa atgcttgaac gatgcttgga tagccctcta gctatttctg
     1381 atttagaaaa tcccatatca agcaaagtat ataagcgtcg tcgatctgag atcgacaaat
     1441 gagtatactt aaccatgcgg cagtctccat tgctgtttca tgttccccaa gaacttgaat
     1501 atacagcatt tttttactat cgcacttcta attagaattt gcccgaagcg tcaataaatc
     1561 tggaagccca ctggaagccc aaccgtaata tctattttgt taaaattgag gatctttaat
     1621 tcgagtgtat tacacgaggt caccagaaat aagaattatt aggctctttc ccaatccggg
     1681 ggcacttcag tttccaaact atgagcaaag cactttaaga ctctatgcca aatccgggca
     1741 cttcaatttc caaactacgc cagtgcttga gactatacgt ttgcgtcatt cccgtgaaaa
     1801 cgggaatcca agtcttcatc ttaggaatac ttggctctat cccaatctgg gggcacttca
     1861 ggttccaaac tataacttgc ataaattagc tcgaactttg cgcagattta gagttttgca
     1921 tggattcaat ttctttaacc atctagaaaa ctagcaataa tatcaagctg actacctatg
     1981 gcttcaatgt agctgcgaag cgtagagata tacatgtcag ttctttgctc aagacgagaa
     2041 atattagctt tctcggtaga taaaacttca gctatgttct tttggctcaa ttgacgtgct
     2101 tttcgaagtt cttgcaaaag catttcgcta agcattgttt agcttttttt atcagaattc
     2161 ttttgagcct gtggcgacat tttatcgcgt aaatttttga aaggtttagc cattgtgtta
     2221 ccctcccttc tttaaagctt caatatgttc attatataaa tcgtcagcta tgagaacata
     2281 ttcctcatac caacgattat ttcctgtttt atcccctcca ataaggagga tcccaacacg
     2341 ttttgaatca aaagcataaa gaatgcgggg atcaatggat caaaacactc tcatatgcga
     2401 gaactaagag ttcaacataa aggaaggtcc tatcgcctga agagtggagg aattttaagg
     2461 ccccattgcc ctaacaacct aatggaagca tcaatatcgg tttgctcccc ttcggttaga
     2521 agatcctctt gtttatgggt tacatacttt tttgcaaggt atttctaagg tacaatttgc
     2581 tctaggccta aaacagaatt cctcttccta ttgcaaatca aatcgaacaa gttaaagaat
     2641 caattgggat gaattaggtt aatttgtttg agatctaatt ttatcccatt gtagctttct
     2701 cacggagatg aaaaaaattt aaactctgac cttattcaat tctgaataac attacgtgaa
     2761 gaccattagc gttatgcttt aacaaatcag cacattattg ttcaactgag tggatcttat
     2821 ttcatgtaag gccggcgcat tttggtccaa attataatca ctatttggtt aaaatgaaga
     2881 atttctttat ttattattaa ggtgtgtgtg cgttggaatc aaaactaatc ttgctttcta
     2941 tactgctcaa taatacggga actaaatatc aaatatggaa ctgataaaaa aatggaccga
     3001 tttttaacat atttcaagct taatgatgta aagaaatagg ctagattgta tgccagaaat
     3061 tactaaaatt gtaatggtct acgccctttt cgttgatgta gatccaaaat atttattaat
     3121 atctttttgc aatttcatat tatttttaaa acattttata tattttgcat cattagaaat
     3181 ttcatcaaga tttgacgctt gtaatttttt ttcagaaata ttatgatttt gaaagcccga
     3241 tgaaaccaaa aaatcgcaaa acatatctag aagttttatg ttcggcaaca tctcttcgat
//
LOCUS       DASTIB010000045         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_637644, whole genome shotgun sequence.
ACCESSION   DASTIB010000045 DASTIB010000000
VERSION     DASTIB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637644"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2341
                     /locus_tag="VFP93_00745"
     CDS             <1..>2341
                     /locus_tag="VFP93_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843166.1"
                     /translation="AFLIQDLLPIKEEDVDSLVKQLVGTSLNETHMDKEITKSEAVTV
                     SLEEILQRQDYKNMLKAFLTMQPNKNPGLVEKEVQERVAKNILTTRWDEFLQTTITDE
                     DARFNAVQENVAEGMEKFKKDLPASPNLTTQTVQAQEQHVTSSTSAAKNADDAKQLSE
                     EQQIEYSKRQAEIMNPKEYDRNKFKIKLRVDFDLALERLNRLIQQGSSEEIRVEAQQA
                     KDAIRNYYYNKAKALDKRIGKKSSSSSSQNEEHEEYLRRIQACGISADLALNPRRLHE
                     DVELARINPSWLIKILATFEHIEEDFKLTGAQANKELLEARGLMLAQLFSHYLEESYE
                     YNNLLAKSNTDLSKAIEDYFIIVKVQDTDDIQIASAMDKVRKIYKQMVDEQIKMQVSF
                     WRYLEERKIPFYENDKLYLQEILASDKLVNKFVSDAKRELLTQISSKEVQMYYMEKDY
                     AELMQSFEMAKRSSEDRIQDYLANLIKNFRVLQEKSRKDTSKHSLARRQMHLIQETTA
                     EVGNILQEMKRQESAGTVSLEQYKKWNEVINHISANLDEQLKKDTQTKGSKFKKLFEP
                     NPKKYAFTQEQLQRVKSDSTKTQFSYDRTREYQQESIKLSEFTPMQLKEKKESNQISR
                     VTSAEVRMATNFLSSHKNPDELGLKDYLQPGTIVKIDDYTINPIKVFFTNPFDDISKV
                     LYIPANKIKKFAKKQLNILEELTPPPNFSVLLNYFPSGSIDANGDPTPPSWQDPFFVS
                     LLEVFMRDPDIIRIVMRGHYSSYDIIQEELNDRIQKNRNL"
BASE COUNT          901 a          386 c          433 g          621 t
ORIGIN      
        1 gcatttttga tccaagactt attgcctatc aaagaagagg acgtagatag tttagtaaaa
       61 caattagtag gaacatcgtt aaatgaaaca cacatggata aagaaattac caaatcagaa
      121 gccgtaacgg tttctttgga agaaattctt caaagacaag attataaaaa catgttgaaa
      181 gcattcttaa ctatgcaacc taataaaaat cctggacttg tagaaaaaga agtacaagaa
      241 cgggtggcaa aaaatattct taccacacgc tgggatgaat ttctacaaac taccattaca
      301 gatgaagacg cacgttttaa tgcagttcaa gaaaatgtag ctgagggtat ggaaaaattt
      361 aaaaaagact taccagcatc acccaacctt acaacacaaa cggttcaagc tcaggaacag
      421 catgttacca gtagcacatc cgctgcaaaa aacgcagatg atgctaaaca attgtcggaa
      481 gaacagcaaa tcgaatattc taagcgtcaa gccgaaatca tgaatcctaa agagtatgac
      541 cgaaacaaat ttaagataaa gctgcgtgtg gattttgatt tggcgctaga gcgtttgaat
      601 cgactgattc aacaaggttc ctcagaagaa atacgcgttg aagcccaaca ggcaaaggat
      661 gctattcgta attattatta taataaagca aaagcacttg ataaaagaat tggcaaaaaa
      721 tcaagttctt caagcagtca aaacgaagag catgaagaat atttacgtag aattcaagct
      781 tgtggcatta gtgcggattt agcgcttaat ccacgaaggt tgcacgaaga tgtcgagctt
      841 gcccgtatta atccctcttg gcttataaaa attttagcta cttttgagca tatagaagaa
      901 gactttaaac tcacgggtgc gcaagcaaat aaagaattgt tagaagcacg tgggctcatg
      961 ctcgcccaat tgttctcgca ttatttagaa gaatcctatg aatataataa tttattagct
     1021 aaaagcaata cagatctttc aaaggcaatt gaagattatt ttattatcgt taaagtgcag
     1081 gatacggatg acattcagat tgccagtgct atggataagg tacgaaagat atacaaacaa
     1141 atggtagacg aacaaataaa aatgcaagta agtttttggc gttatctgga agaaagaaaa
     1201 ataccatttt atgaaaatga caaattgtac ttacaagaaa ttttagccag tgataaattg
     1261 gttaacaaat ttgtgagtga tgcaaaaaga gagttgctga cacaaatttc cagcaaagaa
     1321 gtacaaatgt attatatgga aaaggactat gccgagttaa tgcaatcatt cgaaatggct
     1381 aaacgatcaa gcgaagatag aattcaagat tatcttgcta atttgattaa aaatttcaga
     1441 gtactacaag aaaaaagtcg caaagatact tccaaacatt cattggcacg gcgacaaatg
     1501 catcttattc aagaaaccac cgcagaagtc ggtaatattt tacaagaaat gaagcgacaa
     1561 gaatctgcag gtacggtgtc tttagagcag tataaaaaat ggaatgaagt aataaatcat
     1621 atatccgcta acttggacga acaattaaag aaagataccc aaaccaaagg tagtaagttt
     1681 aagaagcttt tcgaaccaaa tccaaaaaaa tatgctttca cgcaagaaca attgcaacgt
     1741 gtaaagtcag atagcactaa aacgcagttt tcctatgaca gaactagaga atatcaacag
     1801 gaaagcatta aactgagtga gtttacgccg atgcaactca aagaaaaaaa ggaaagtaat
     1861 caaatttcta gagttacttc tgctgaagta agaatggcca caaatttttt gagttcccat
     1921 aaaaatccag atgagctggg tttaaaagat tatttacaac caggtactat tgtgaaaata
     1981 gatgattata ctataaatcc cataaaagta ttttttacca acccttttga cgatatatcg
     2041 aaggtattat acatacctgc aaataaaatt aaaaaatttg cgaaaaaaca attaaatata
     2101 ttggaggaat tgacacctcc tccaaatttt agtgtacttc tcaattattt tcctagcggt
     2161 tctattgatg ctaacggaga cccaactcct ccttcttggc aggatccgtt ttttgtatcg
     2221 cttctagaag tttttatgcg cgatccggat ataattcgaa tagttatgcg tgggcattac
     2281 tcctcgtatg acattattca agaagagtta aacgatagaa tacaaaaaaa tagaaatttg
     2341 g
//
LOCUS       DASTIB010000046         4873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1278239, whole genome shotgun sequence.
ACCESSION   DASTIB010000046 DASTIB010000000
VERSION     DASTIB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278239"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /gene="rpsK"
                     /locus_tag="VFP93_00750"
     CDS             complement(<1..383)
                     /gene="rpsK"
                     /locus_tag="VFP93_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9843167.1"
                     /translation="MSIESSQVEEAKTDETDEEGGRGKGGVESGKGKPRRQKKYKRLV
                     QDIIVHIYATFNNTVVTVTDVKGNVLSWATAGGSGFRGSRKSTPFAAQVAVDRALRKA
                     KELYGAESAEIRVCGPGPGRDAATR"
     gene            complement(410..778)
                     /gene="rpsM"
                     /locus_tag="VFP93_00755"
     CDS             complement(410..778)
                     /gene="rpsM"
                     /locus_tag="VFP93_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9843168.1"
                     /translation="MATVRIQGVPLSPNKHIRIALTDIYGIGGPRALKICESAGIEPQ
                     RKVSELSDQEVHRVAEECGRYRLEGELRREVQLAVKRLRDIGCYRGLRHRNGLPVRGQ
                     RTRTNARTRKGPRGSRVGKK"
     gene            complement(896..1012)
                     /gene="rpmJ"
                     /locus_tag="VFP93_00760"
     CDS             complement(896..1012)
                     /gene="rpmJ"
                     /locus_tag="VFP93_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9843169.1"
                     /translation="MKVRASIKKMCKGCKIVARKGKKFVICETSPKHKQRQG"
     gene            complement(1025..2350)
                     /gene="secY"
                     /locus_tag="VFP93_00765"
     CDS             complement(1025..2350)
                     /gene="secY"
                     /locus_tag="VFP93_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9843170.1"
                     /translation="MAAKNVRQMSGLEELKRRLWYLVFGLIIYRIGAHIPIPGLDADQ
                     LQKLFSGTSGLFGLFNLFSGGALQRLSIFALGIMPYISASIIMQLLTVVSPQLEQLKK
                     EGETGRRKLTQYTRVGTLALATIQGSAMASFIASQSPTIVPSFIFYFTTVITLVTGTM
                     FLMWLGERITERGIGNGISMIIFAGIVAGLPNAIASTFEQVRQGAMQPLALIVLLTVL
                     VVVTGFIVFVERGQRRITVNYAKRQQGNRLYAQQQSHLPFKVNMAGVIPPIFASSIIL
                     FPATIGKMFGGSTDSSILTDISLALSPGQPLYILLFGAAILFFAFFYTALVFNPRETA
                     ENLKRGGAFIPGIRPGEQTASYIDKVMTRLTFVGGMYIMAVSLLPEFLILAWQVPFYF
                     GGTSLLITVVVLMDFIAQVQAHLMSQQYESLIKKANLKSRDSGGGFGLF"
     gene            complement(2356..2796)
                     /gene="rplO"
                     /locus_tag="VFP93_00770"
     CDS             complement(2356..2796)
                     /gene="rplO"
                     /locus_tag="VFP93_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET9843171.1"
                     /translation="MKLNTIRPAEGAKKSATRVGRGPGSGIGKTCGRGIKGQKARAGG
                     YHKVGFEGGQMPLQRRLPKYGFTSQKALVSQDIRLSDLNYIEGNIVSLETLKDANIIP
                     KSIKFAKIYLSGEIKRALTIQNLKITKGAKEAILAQGGNIEENS"
     gene            complement(2793..2990)
                     /gene="rpmD"
                     /locus_tag="VFP93_00775"
     CDS             complement(2793..2990)
                     /gene="rpmD"
                     /locus_tag="VFP93_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003014363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET9843172.1"
                     /translation="MKGATENRIKVTLKKSLAGRKQSHIACARGLGLRRIHQTVEVID
                     TPENRGMIREIGYMVSVEGEV"
     gene            complement(2974..3501)
                     /gene="rpsE"
                     /locus_tag="VFP93_00780"
     CDS             complement(2974..3501)
                     /gene="rpsE"
                     /locus_tag="VFP93_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9843173.1"
                     /translation="MPKKQEEKTQGDYEERLIKVRRVAKVVKGGRVFGFSALVVVGNR
                     NGRVGMGHGKAREVPEAIRKAMDAAKRNLIEVPMNGSTLHHTIQSSHGASKIFMKPAS
                     EGTGIIAGGAMRAIFEVAGISNVLSKCYGSTNPVNVALATIKGLKGSHSPQTVAEKRG
                     LKVDEIMGGAHEGRN"
     gene            complement(3512..3883)
                     /gene="rplR"
                     /locus_tag="VFP93_00785"
     CDS             complement(3512..3883)
                     /gene="rplR"
                     /locus_tag="VFP93_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9843174.1"
                     /translation="MNSKTIKRFRRAKRSREHIKKLALEKGIARLSIYRSSSHIYAQI
                     LAPIGGKVLAHASSLEKDIKAEKNAEGGKIGVAQNVGKLIAKRAQEAGIERVACDRSG
                     YRYHGRVAALVEAARETGLVV"
     gene            complement(3895..4455)
                     /gene="rplF"
                     /locus_tag="VFP93_00790"
     CDS             complement(3895..4455)
                     /gene="rplF"
                     /locus_tag="VFP93_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9843175.1"
                     /translation="MASRVGRKPIDIPKGVEIKVEKSLVQVKGAKGTLTLDLPQGIMV
                     HQDGGELKVVAEESLENADAATGTVRAVLNNHVKGVCEGFTKKLQLVGVGYRAQAGKT
                     GNRHKLDLTLGLSHPVTYLAPEGIELATPSVTEILVTGIDKQLVGQVAADVRNIHRGI
                     RKPEPYKGKGIRYANEKIILKETKKK"
     gene            complement(4468..4863)
                     /gene="rpsH"
                     /locus_tag="VFP93_00795"
     CDS             complement(4468..4863)
                     /gene="rpsH"
                     /locus_tag="VFP93_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9843176.1"
                     /translation="MSLQDPIADMLTRIRNAQSSRKHEVFLPSSKLKKAILAIMKAEG
                     YIADFQVTTNNNKDELRVELKYHRGRPVIELLKRVSKPGLRHYLGKDELPKIMDGLGI
                     AIVSTSNGVMSERDARAAGHGGEILCYVA"
BASE COUNT         1393 a         1180 c          815 g         1485 t
ORIGIN      
        1 tcacgagtcg cagcatcacg acctggtcct ggtccacata ctctgatttc agcactttct
       61 gcaccataaa gctctttagc ttttcttaag gcgcgatcaa ccgctacttg tgctgcaaat
      121 ggagtgcttt tacgtgaacc acgaaaacca ctaccacccg ctgttgccca agataatacg
      181 ttccccttga catcagtgac agtgacaact gtattattaa atgtggcata gatatgaaca
      241 ataatatcct ggactaaacg cttatatttc ttttggcgtc taggcttgcc ttttcctgat
      301 tctacaccac ccttcccgcg tccaccttct tcatcggttt catcggtctt ggcttcttca
      361 acttgactac tttcaattga cataaatcac atccaacgtc gtaaatcggt tattttttcc
      421 caactcggct accccgagga cctttacgtg tgcgagcatt agttcttgtt ctttgaccac
      481 gcactggtaa cccattacga tggcgtaatc ctctataaca cccaatatca cgtaaacgct
      541 taactgctaa ttgtacttcg cgacgtaact cgccttctaa acgatagcgg ccacactctt
      601 cggcgacacg atgtacttct tgatctgaaa gttcagatac ttttctttgt ggctcaatgc
      661 cagcagactc gcaaattttt aaagcgcgcg gcccgccgat gccataaata tcagtcaaag
      721 caattctaat atgcttattt ggactaagtg gcacaccttg tatacgtact gttgccatct
      781 atgataaacc ccctaaaact ttggtgccca acaaaacaag aagcctactt gagactatgg
      841 ataaagtcaa aaattggcga ttaattaacg acataaatcg taccatgtaa aaatattagc
      901 cttggcgttg cttatgctta ggactagttt cacaaataac aaatttttta cccttccgag
      961 ctacaatttt acagccttta cacatttttt taatggatgc acgaaccttc atttatgtta
     1021 tctcctaaaa taatccaaaa ccaccaccgc tgtcgcgact ttttaagttg gcttttttaa
     1081 tgagagattc gtactgctgc gacattaaat gcgcttgaac ttgagcaata aaatccatca
     1141 acacaactac cgtaattaat agtgaagttc caccaaaata aaagggcact tgccaagcca
     1201 atattaaaaa ttcaggcaac aaagaaaccg ccatgatata cataccgcct acaaaagtaa
     1261 gccttgtcat cactttatct atataactag cagtttgttc acctggtctt atgcctggaa
     1321 taaacgctcc accacgtttt aaattctctg cggtttcacg aggattaaaa actaaagcag
     1381 tatagaaaaa tgcaaaaaat agaatggctg ctccgaatag taagatatat aaaggttggc
     1441 caggacttaa agccaaacta atatctgtca atatactgga gtcagtcgat ccaccaaaca
     1501 tttttccaat ggttgcagga aacaaaatta tacttgaagc aaagatgggt ggtataacgc
     1561 ctgccatatt tactttaaaa ggcaaatggc tttgctgttg agcgtataat cgatttcctt
     1621 gctgacgttt agcatagtta acagtgattc ttctttgacc tctttctaca aatacaataa
     1681 agcctgtcac gactactaaa actgttaata aaacaattaa agcaagtggt tgcattgctc
     1741 cttgtcgtac ttgttcgaag gtacttgcta ttgcattagg taagccagca acaataccag
     1801 cgaagataat catagaaata ccgttaccta taccccgttc cgtaatcctc tcccctaacc
     1861 acattaaaaa catggttccg gttaccaagg ttataacggt tgtaaagtaa aatataaaac
     1921 ttggaactat agttggactt tggcttgcaa taaaacttgc catcgcagat ccttggatgg
     1981 ttgccaaggc taatgtaccg acgcgggtat attgcgttaa tttacgacgg cccgtctctc
     2041 cctctttttt cagctgttct agctgaggtg ataccacagt tagaagttgc atgattatag
     2101 aagcagatat atacggcata atacccaatg caaagattga taatctttgc aaggcgccgc
     2161 ctgaaaataa attaaataag ccaaataaac cactagtgcc agaaaataat ttttgtaact
     2221 ggtctgcatc aagaccaggt atgggaatgt gtgcaccaat tctataaatg attagaccaa
     2281 agaccagata ccacaatctg cgctttaatt cttctaatcc actcatttga cgcacattct
     2341 tagcagccat ggttattaag aattctcctc aatattacca ccttgcgcaa gaattgcttc
     2401 cttcgcaccc ttcgtaatct taagattttg gatggttaga gctcttttta tttcacccga
     2461 caaataaatt tttgcgaact taatggattt tggaattata tttgcatctt tcaatgtttc
     2521 caaggaaacg atattacctt caatgtaatt taaatcgctt aatcgaatat cttggcttac
     2581 taatgccttt tgcgaggtaa atccatattt gggtaagcga cgttgaagtg gcatttgacc
     2641 accctcaaaa cctactttat ggtagccacc agcgcgtgct ttttgacctt taataccacg
     2701 accacaagtt ttacctatac cacttcctgg accacggcca acccgtgtcg cgcttttttt
     2761 ggcgccctct gctggtcgaa ttgtattcag tttcatactt caccttctac agataccatg
     2821 tagcctattt ctcgaatcat gccacgattt tcgggtgtat cgataacttc cacggtctga
     2881 tggatacgac gcaagcccaa accacgcgca caagcaatat gagactgctt tctaccggct
     2941 aaactctttt ttaaagttac tttaattcta ttttcagttg cgcccttcat gagccccacc
     3001 cattatttca tctactttca agccacgctt ctctgccact gtttgtggag aatgagagcc
     3061 ttttaaccct ttaatagttg ccaatgccac attcacaggg ttggttgaac cataacattt
     3121 ggataataca ttgctaattc cagccacttc aaatatggca cgcatcgccc ctccggcaat
     3181 gataccagta ccttccgatg caggtttcat gaaaattttt gaagcgccgt gagaagattg
     3241 aatggtgtga tgtaaagtcg aaccattcat aggaacttca attaagtttc ttttagctgc
     3301 atccatcgcc ttacgaattg cttcaggaac ttctctggcc ttgccatgtc ccattcctac
     3361 acgaccatta cggttaccaa caaccactaa ggcagaaaaa ccaaaaacgc gaccaccttt
     3421 cacaaccttg gcaactctac gtactttgat taatctttct tcataatcac cctgagtctt
     3481 ctcttcttgc ttcttaggca ttacattttc ctcaaacaac taaaccagtt tcacgtgcag
     3541 cttctactaa cgcagcaaca cgtccgtggt atcggtatcc agaacgatca caagccacac
     3601 gttcaatacc tgcttcttgt gctctctttg caattaattt gccaacgttt tgagcaacgc
     3661 cgatttttcc gccttccgca tttttctcag ctttaatatc tttttccaaa ctggatgcat
     3721 gcgctagcac ttttccacca ataggagcta aaatttgcgc ataaatatga gaagaagaac
     3781 ggtaaataga caagcgcgct attcccttct ctaaggcaag ctttttaata tgctcacgcg
     3841 accgctttgc acgtcgaaat cttttaatag ttttactatt catattctac ttacctactt
     3901 cttcttggtt tccttcagaa taattttctc atttgcgtac cgtattccct ttcctttgta
     3961 aggctcgggc ttacgaattc cacgatgaat attccgaacg tcagcggcta cttggcctac
     4021 caattgctta tcaatacccg ttaccaaaat ttccgtaacc gatggtgttg ccaactctat
     4081 accttcaggc gccaaataag taacaggatg cgacaatcct aaggttaaat ctaatttgtg
     4141 gcgatttcct gttttaccag cttgcgcacg ataacctacg ccaaccaatt gcaatttctt
     4201 cgtgaaacct tcgcaaacgc ctttaacgtg attatttaat actgctcgaa ctgttccagt
     4261 cgcagcatct gcattttcta aagattcttc agcaacaact tttaattcac caccatcttg
     4321 gtggaccata atcccttgtg gcaaatcaag tgttaaagtg ccttttgcac ctttaacttg
     4381 aaccaaagat ttctcaactt taatttcaac gccctttgga atatcaatgg gctttctacc
     4441 tactcttgat gccatgagaa cagtcctcta agcgacataa cataagatct caccaccatg
     4501 tcctgctgca cgtgcgtcgc gctcagacat cactccattc gaagtggaaa caatggcgat
     4561 acccaaacca tccataattt tcggtaattc atccttacct aaataatggc gcaaacctgg
     4621 cttagaaacc cgttttagta attctatcac tggacggcca cggtggtatt ttaattcaac
     4681 acgtaattca tctttattgt tatttgtcgt aacttgaaaa tccgcgatat acccttctgc
     4741 tttcataata gctaaaattg ctttctttag cttagatgaa ggcaaaaaaa cctcatgctt
     4801 tctagaggat tgcgcattgc ggatccgagt taacatatca gcgatcggat cttgtaaact
     4861 catttcaaac ttc
//
LOCUS       DASTIB010000047         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_637502, whole genome shotgun sequence.
ACCESSION   DASTIB010000047 DASTIB010000000
VERSION     DASTIB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637502"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFP93_00800"
     CDS             <1..375
                     /locus_tag="VFP93_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET9843177.1"
                     /translation="LDENVLEYANFMIDEHTKNQQQTEEVAKEAQITAIKDKDAKKLE
                     KKGKEGLEKMQGLDDKKFQKEYIKTMIHDHKDALKAIDKNYLPEIKNQELKAHFSETR
                     KHVEDHLNKAKELKENLDDKKS"
     gene            394..>2226
                     /gene="cphA"
                     /locus_tag="VFP93_00805"
     CDS             394..>2226
                     /gene="cphA"
                     /locus_tag="VFP93_00805"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9843178.1"
                     /translation="MKALEIRVLRGPNYWSDTHSKVILIKIDLEEFKNVTSDQIKDFN
                     SNLQILLPSLATHKCFAGEPGSFFDKLAEGLLLGHIIEHVALELQCLAGMRCGYGKTR
                     YSADSGIYTIVFSYEHERAGQFAAKTALNITNTLASNTPYVSLDQDIQFLKTIAEQEK
                     LGPSTDALVREAKRRSIPYTRLDSLSLVMFGQGCNRKMIRSTITTNTSLLGADFAADK
                     DVTKQFLASELIPVPVGEVVASLEELDKAIQRIGFPVVVKPQNANHGRGVTTNIKTRD
                     MAVYAFTLAKHISDAIIVEKFINGQDYRILVVNYKFVAAAKRESPLITGDGKRTIQQL
                     IDEINQDPLRGSGHDNILTKIEIDEATKAIMEEKGLDPNSILKENQPLKLKYAANIST
                     GGTAEDVTSLMHPSNVFLAERIARIMNLDICGIDLIAEDIAVPIKHTNGAIIEINAAP
                     GLRMHLQPTKGKKRNVARKILDMLYPAESTPFIPITAVTGTNGKTTVVRLIAHIAQSV
                     KRQVGFTTTEGIYINNFEVYRGDCSGPSSAGVVLRDPMVDFAVFECARGGILRSGLGF
                     NQCDVSVVTNVSSDHLGIQEIHTIEEMAKVKSVIPRQTKSEGYAI"
BASE COUNT          778 a          395 c          457 g          596 t
ORIGIN      
        1 ttggatgaaa atgttttgga atatgcaaac ttcatgattg atgagcatac aaagaaccaa
       61 caacaaacgg aagaagtcgc aaaagaggct caaattactg ccatcaaaga taaagatgct
      121 aaaaaattag aaaaaaaagg aaaagaaggt ctggaaaaaa tgcaaggtct tgatgacaaa
      181 aagtttcaaa aagaatacat caaaacgatg attcatgatc ataaagatgc gttaaaggct
      241 atagataaaa attatttacc tgaaattaag aaccaagaat tgaaggcaca tttttcagaa
      301 accagaaaac atgtcgaaga tcatttaaat aaagccaaag aattaaaaga aaatctcgac
      361 gacaaaaaga gttaaatatg ttagagggca agcatgaagg cattggagat aagggtttta
      421 cgaggtccaa attattggtc agatacgcat agtaaagtaa tcttgatcaa aatagatctg
      481 gaagaattta aaaatgttac ttccgatcaa attaaggatt ttaactccaa tctccaaatt
      541 ctgctgccct cgctcgcaac ccataaatgt tttgcgggag aaccgggtag tttttttgat
      601 aaacttgcag aagggctatt attaggacat atcatcgaac atgtcgcact tgagttacaa
      661 tgtttagcag gaatgcgatg tggttatggt aaaacacgct atagcgcaga ttctggcatt
      721 tatactattg tattctctta tgaacatgaa cgcgctggac aatttgcagc taaaactgcg
      781 ttaaatatta ccaatacact cgcctcgaat actccctatg tatcattaga tcaagatatc
      841 caatttttaa aaacgatcgc ggaacaagaa aaactaggcc caagtacgga tgctttagtc
      901 cgtgaagcaa agcgaagaag cataccttat acgcgattag attctctttc tttagttatg
      961 tttggacaag ggtgtaatcg caaaatgatc cgctctacga ttaccaccaa tacaagtctg
     1021 ttgggggcgg attttgcagc cgataaagac gttaccaaac aatttctggc gagtgagctt
     1081 attcctgtgc ctgtaggcga ggttgtggct tctttagaag aattagataa agctatccaa
     1141 cgtattggat ttcctgtggt agtaaaacca caaaatgcta atcatggtcg cggagtgacg
     1201 accaatatta aaacgcgcga tatggccgtt tatgctttta cccttgccaa acatatttca
     1261 gacgcaatca ttgttgaaaa atttatcaat ggtcaagatt atcgaatttt ggttgtaaat
     1321 tataaatttg tggctgcggc aaaacgtgaa tcacctttga taacagggga tggtaaaaga
     1381 actatacaac aattaattga cgaaataaat caagatccat tacgtggatc cggtcacgac
     1441 aacatcctta ctaaaattga aattgatgag gctaccaaag caattatgga agaaaaaggt
     1501 ttagatccga attccatctt aaaagaaaat caacccctta aattaaaata tgctgctaat
     1561 attagtaccg gtggaacggc cgaagatgtc acaagcttaa tgcacccgag caacgttttt
     1621 ttagccgaac gcattgcccg aattatgaat ttagatatat gcggcatcga tttaatcgcc
     1681 gaggatattg cggttcctat caaacatacg aatggagcta tcattgaaat taatgcagct
     1741 ccaggtttgc gcatgcattt acaacccact aagggaaaga aacgcaacgt agcgcgaaaa
     1801 attctcgata tgctgtatcc tgcagagagt accccattca taccaattac tgcggtgact
     1861 ggaacaaatg gaaaaactac agttgttcgt ttaattgcgc atatcgctca aagtgtcaaa
     1921 cggcaagtag gattcaccac caccgaaggc atttatatta acaattttga agtttaccgg
     1981 ggagattgta gtggaccttc aagtgctgga gtggtgttac gtgatcccat ggtggacttt
     2041 gcggtattcg aatgtgctcg aggtggtatt ttacgatctg gattagggtt taatcaatgt
     2101 gatgtcagcg tggtaacaaa cgtttcaagt gatcatctag gcatacaaga gatccatact
     2161 atcgaagaaa tggctaaagt aaaatccgtg attccaagac aaacaaaatc agaaggatac
     2221 gccatt
//
LOCUS       DASTIB010000048         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_100606, whole genome shotgun sequence.
ACCESSION   DASTIB010000048 DASTIB010000000
VERSION     DASTIB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100606"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1468)
                     /gene="pcnB"
                     /locus_tag="VFP93_00810"
     CDS             complement(131..1468)
                     /gene="pcnB"
                     /locus_tag="VFP93_00810"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HET9843179.1"
                     /translation="MGVFSKGVTGVSISASPKSLKPIMQIIPRSEHSISRSDISENAL
                     KVLYRLKKHNFEAYLVGGGVRDLLLGHHPKDFDVVTDAHPEQIRSIFNNCRLIGRRFR
                     LAHVFFGHDIVEVATFRGKSVEEKSFNTSTEGMLLRDNVYGTLQEDVWRRDFTVNAIY
                     YNIKDFSLVDYTGGLKDIEERVIRIIGEPSNRLREDPVRMLRAIRFSCKLNFTLAENL
                     SKSMHELGNLLKQVPSARLFEEVIKIFHSGHALAIFRQLRFYKLLSYLFPTINAVNDE
                     NKQLQFIENVLQNTDDRILNHLTANPAFLFAALLWHPLQHCIEQMQENHSLSRFSATL
                     QAMEQILHAQRASLSFPRRLSIMMKEIWILQIRLERCAPKQVKRITNDPRFKSAYDFL
                     LLRYSAQDVTQEIIQWWQNHVPEEDYTHKPYQKSKKRRGSKRKKKPLDQDFTG"
     gene            complement(1625..2050)
                     /locus_tag="VFP93_00815"
     CDS             complement(1625..2050)
                     /locus_tag="VFP93_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843180.1"
                     /translation="MGTVVEKTPAISQKEWRKLETISNNIMRTYHNLHQDVKQHSYEA
                     ILVQSLRFAKEVMHYINELRHFCNNVAHNSTYQVQEKAQFLNCIEEYIKNLKTLSLSY
                     KRNENFMHTRFRFIVEDEETRLIKLEQAIQSVKGLLQIH"
     gene            2204..2848
                     /locus_tag="VFP93_00820"
     CDS             2204..2848
                     /locus_tag="VFP93_00820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9843181.1"
                     /translation="MILEETLLSNLISQYAVKMTAKEPTLLENLRQETLTQTNAPQML
                     SGPVVGQLLHLFVQILDAKHIIEVGTFTGYSALYMASALRDGKLITCEIDERHALIAK
                     SYFDRSPHGHKIDLRIGPALKTLEHVSGPFDLAFIDADKKNYPNYYEKLLPKIRKGGM
                     IIIDNALWSGRVLEPLDLESKAIASLNEKASQDSQVETLMLAIRDGLLICRKND"
     gene            complement(2851..>3157)
                     /locus_tag="VFP93_00825"
     CDS             complement(2851..>3157)
                     /locus_tag="VFP93_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843182.1"
                     /translation="FLGKLYQATHDVNHLRTAQTLQEWYKTAAFSTEGEIIWGYSVLA
                     NITQEQRYLQQAQNFAQEIIQLQQLNGLWTKLDKKELLSSARHAIFLKESIPELSSL"
BASE COUNT          961 a          576 c          570 g         1050 t
ORIGIN      
        1 tataaaatct tgattttttt aacagcacaa tttggggtac ttcttttaag tacgcaattg
       61 cttgctctac ttgttttaaa ggaagttcta agttactccc caggcctatg taagccgtag
      121 ccatccattt ttaccccgta aaatcttgat ccagaggttt ttttttgcgt ttggatcctc
      181 tgcgtttttt gcttttttga tagggtttat gcgtgtaatc ttcttctggc acatgatttt
      241 gccaccattg aataatttct tgtgttacat cttgagcaga ataccttaat aataaaaagt
      301 cataagcact tttaaaacgt ggatcgtttg ttattctttt tacttgttta ggagcgcaac
      361 gttccaatcg tatttgtaaa atccatattt ctttcatcat aatacttaaa cgtcttggaa
      421 agctcaaact agcacgttgt gcgtgtaaaa tttgctccat agcttgtaaa gttgcagaga
      481 aacggcttaa agaatgattt tcttgcattt gttctatgca atgctgtaaa ggatgccaca
      541 acagtgctgc aaacagaaaa gcaggattag cggttaaatg gttaagaata cgatcgtctg
      601 tattttgtaa aacattttcg ataaattgca gctgtttatt ttcatcattt accgcattaa
      661 ttgtgggaaa taaataagac aatagtttat aaaaccgcag ttgccgaaag atggctaagg
      721 catgacctga atgaaatatt tttataactt cttcgaataa acgtgcactc ggcacctgtt
      781 tgaggagatt gcccaactcg tgcatggatt ttgataaatt ttctgccagc gtaaaattta
      841 atttacaaga aaagcgtatg gcccgaagca ttcttacagg atcttcgcgc agacgattag
      901 agggttctcc tataattctt atgacacgtt cttcaatgtc ttttaaacca cctgtatagt
      961 cgactaaact aaaatctttt atgttgtaat agattgcgtt tacggtaaaa tctcgacgcc
     1021 aaacatcttc ttgcaaggta ccatatacat tatcacgcaa tagcattccc tcggtggacg
     1081 tattaaacga tttttcttca acagacttgc cacgaaaagt cgctacctcc acgatatcat
     1141 gtccaaagaa tacgtgtgct aaacgaaatc gccgtccaat taaacggcaa ttgttaaaaa
     1201 tggatcggat ttgctccgga tgcgcatcgg ttacaacatc gaaatcttta ggatgatgtc
     1261 ctaaaagaag atctcgtact ccccctccaa caaggtatgc ttcaaaatta tgttttttta
     1321 agcgataaag cacttttaaa gcattttcgc ttatatcaga acgtgatatt gaatgttcac
     1381 tgcgtggaat gatttgcata atcggtttta gagatttagg agaggcactt atacttaccc
     1441 ccgtaacgcc tttggaaaat acgcccatta atttaagcag ccgttggaac atagcttttt
     1501 agatgaataa aactaatcat cgtaacttac aaaactcagg caggtatgat accgcaagga
     1561 tatcaacgtt tgctagtgac tttatttaag caagccaata aacttatacc gaaaaacaat
     1621 gatcttaatg tatttgcaat aaacctttaa cagactgtat agcttgttct aacttgatta
     1681 atcgggtctc ttcatcttca actataaaac gaaaccgtgt atgcataaag ttctcattgc
     1741 gcttataaga caatgagagc gttttaagat tcttaatgta ttcttcaata caatttaaaa
     1801 attgggcttt ttcctgaact tgatacgtcg aattatgggc tacattgtta caaaaatggc
     1861 gaagttcgtt gatataatgc atgacttctt tggcaaatcg taaactttgt accaaaattg
     1921 cctcgtaact atgttgtttt acatcctgat gaagattgtg atatgtgcgc attatattgt
     1981 ttgaaatagt ctcgagcttg cgccattctt tttgagaaat ggcaggtgtt ttttctacaa
     2041 ctgttcccat attttatctc ctatttttgt tggataactt tgacctatta ctaaaacagc
     2101 ttccttcgtt tttatttttg ttttgaccca aaaggccacc aaaggttcaa atgcgtttga
     2161 taacccgtac agagtccgat aagatattta ttggagaatt attatgattt tagaagaaac
     2221 attactgagc aatttaatct cgcaatatgc ggtaaaaatg acggctaaag aacccacttt
     2281 attggaaaac ttgcgtcagg aaaccttaac ccaaaccaat gccccacaaa tgttgagtgg
     2341 tcctgttgta ggacaacttc tccatttatt tgttcaaata ctagatgcaa agcatattat
     2401 agaagttgga acattcacgg ggtattcagc actttatatg gctagcgcgt tacgcgacgg
     2461 aaaattaatt acctgcgaaa tagatgaaag acatgcgctt attgcaaaat cgtattttga
     2521 tcgaagtcct catggtcata aaattgatct gagaattggt cctgctttaa aaacgcttga
     2581 acacgtttca ggtccctttg atttagcatt tattgatgca gataagaaaa attatcctaa
     2641 ctattatgaa aaactacttc cgaaaattcg aaaaggtggc atgattatca ttgataatgc
     2701 tctatggagc ggaagggtct tggaaccttt agatttggaa tctaaagcta ttgcctcttt
     2761 aaatgaaaaa gcaagccaag attcccaagt tgaaacttta atgctggcaa ttcgtgatgg
     2821 actcttgatt tgccgaaaga acgattaaca tcacagagaa cttaactcgg ggatactttc
     2881 tttcaaaaaa atggcatggc gtgcggaaga aagtaactct tttttatcta gttttgtcca
     2941 caaaccattt aactgttgta attggatgat ttcctgtgca aaattctgcg cttgttgtaa
     3001 ataccgttgc tcttgcgtta tgttggctaa tacggaatat ccccaaataa tttctccttc
     3061 ggtagaaaat gctgcggttt tataccattc ctgtaaggtt tgtgcagttc ttagatgatt
     3121 tacatcatgc gtagcttgat ataatttacc taaaaat
//
LOCUS       DASTIB010000049         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_428712, whole genome shotgun sequence.
ACCESSION   DASTIB010000049 DASTIB010000000
VERSION     DASTIB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428712"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..2231
                     /gene="prsT"
                     /locus_tag="VFP93_00830"
     CDS             54..2231
                     /gene="prsT"
                     /locus_tag="VFP93_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HET9843183.1"
                     /translation="MQAEIYKQKKDFDSARIAYQHAVDLDNHSIPARLGLTQIYLLQR
                     NVQDFIKQATLLYQFAPKYSGSLYWYSIALYQQKKMDQAREILERLVRFKPQHAPALL
                     LLAKIYYNEERYETAQDTLQKFLTLNPDHKVGKALKAAVHLKLRNPQKTIAILEPLVE
                     ETPHFQWLALLGHAYVLTGNLDKATTTLEKAASSMPEDPTIQMELVGSLLSQQDDRKA
                     ESILDEVVAHHEHYEEAEVLQIFIALKNNNPEKAEGIAKKLLKSNPHNPVYHNLLGLS
                     LIKQERFSSAEEHYKTAIQKAPNFIAARNNLSELYLKQNNYREAEKNLQITLSQDNNN
                     IRALLLHSVLSESKQDFDAALKWLRLAQERNPYDYTITNQIVSFFLRHKKLDRAYEEA
                     LLGLAKFPHLPQAQHLVGQLALNQKQYKKALEIYKKLAQKLPNNTQILQGLARAYVAV
                     GDMHEASEIIEKVLANENGNLSALLIKADMALKSKAYDSVEKIARQIIDNNPKHPLGH
                     RLYGDALFGKGLTKDSVKYYEMAYHLGKKPDYAKVYYHALNHTNESAKGLAVLEQYLQ
                     EYPEDKEMHQFLATTYLTKNQLDKAKHHYEIVLQTKSDDYVSLNNLAHVYLKKNDPFN
                     AYEFAKKAYDLDPENPQVADTFAWTLILRKEPNKAVPILTKVLSNNPHLPDLRYHRAV
                     GLYELGNKRLAISELENLLHNTDPFTDKEQAQKFLHKIKSQQG"
     gene            2239..>2688
                     /locus_tag="VFP93_00835"
     CDS             2239..>2688
                     /locus_tag="VFP93_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9843184.1"
                     /translation="MTHFRYKKICLLVSIALFLVGCSHNYPLLEKGAYTYNLHSSYHI
                     GPGDHLSILVWKNADLTLGVIVRPDGFITLPLVDDIQAAGKTPMVLAEDLEHALAEYL
                     KDPLVTVMVTGFIGTYTDTIRIVGEATQPQSLPYRDGMTVLDLMIVVG"
BASE COUNT          930 a          503 c          501 g          754 t
ORIGIN      
        1 aagcccacgg ctttaaataa caaaatttaa aaatcgactc caaattacaa cttctgcaag
       61 ccgaaattta taagcaaaaa aaagattttg atagcgcgag aatagcttac cagcatgcgg
      121 ttgatttgga caatcatagt attcctgccc gcttagggtt aacgcagatc tatcttctgc
      181 aacgcaatgt tcaagatttt atcaaacaag cgactttact ctatcaattt gcaccaaaat
      241 actcaggtag cttgtattgg tattccattg cgctttatca acagaaaaaa atggaccaag
      301 cgcgtgagat cttagaacga ttagtccgat ttaaaccgca acatgcgcct gcgctgttgc
      361 tacttgccaa aatctattac aacgaagaac gttatgagac tgcgcaagac actttacaaa
      421 aatttttaac attgaatccg gatcataaag taggtaaagc attaaaagct gccgtacatt
      481 taaaattacg taacccacaa aaaacgattg ctattttaga acccctagta gaagaaacac
      541 cgcatttcca atggctagca ttgctcggtc atgcttatgt tttaacggga aatctagata
      601 aagcaacgac aaccttagaa aaagccgcaa gtagcatgcc agaagatcct accattcaaa
      661 tggaattggt gggaagttta ttatctcagc aagacgacag aaaagcagag tccattttag
      721 atgaagtagt agcgcatcat gaacattatg aagaagcaga agtattgcaa atatttatcg
      781 cgcttaaaaa taataatccc gaaaaagccg aaggtattgc taaaaaactt ttaaaaagta
      841 acccgcataa tcctgtatac cataatttat taggcttaag cttaataaag caggagcgtt
      901 tttcctctgc ggaagaacac tacaaaaccg ctatacaaaa agcgcctaat tttatcgccg
      961 cccggaacaa tttgagcgaa ttgtatttaa agcaaaataa ttatcgtgag gccgaaaaaa
     1021 atctgcaaat tacattaagt caagataata acaatataag agccttatta ttgcatagtg
     1081 ttttgtcgga atccaaacaa gattttgatg ctgcactaaa atggttgcga ctcgcgcaag
     1141 aacggaatcc atacgattat actattacca accaaatagt aagttttttt ctgcggcata
     1201 aaaaattgga ccgagcttat gaagaagctt tattaggatt agcaaaattt ccccatttgc
     1261 cgcaagccca gcatttggta ggacaacttg cattaaacca aaagcaatac aaaaaagcct
     1321 tggaaattta taaaaagctt gcacaaaaac ttcctaataa tacccaaatc cttcaaggtc
     1381 tagcgcgagc ctatgtggcg gttggtgata tgcatgaagc ctctgagatt attgaaaagg
     1441 ttttggcgaa cgagaatggt aatttatctg ctttgcttat caaagcagat atggccttaa
     1501 aatcgaaagc ttatgattcc gttgaaaaaa tagcgcggca aattatcgac aataacccta
     1561 agcatccttt agggcatcga ttatacggtg atgcgctatt tggaaaagga ttgacaaaag
     1621 attctgttaa atattatgaa atggcctatc atttaggcaa aaaaccggat tatgctaaag
     1681 tctattacca tgctttaaat cacacgaatg aaagcgcgaa aggcttggct gttttagaac
     1741 aatatttaca ggaatatccg gaagataaag aaatgcatca gtttttagca actacatatc
     1801 ttactaaaaa tcaattggac aaagcaaagc accattatga aatagtattg caaactaaaa
     1861 gtgacgatta tgttagttta aataacttgg cgcatgttta cttaaaaaag aatgatccct
     1921 ttaatgccta tgaatttgcc aaaaaggctt atgacttaga tccggaaaat ccccaggttg
     1981 cagatacatt tgcttggact ttaattttac gtaaggaacc aaataaagca gtgcccattt
     2041 taacaaaagt tttgtcaaat aatccgcatt taccagatct tcgttatcat agagccgtgg
     2101 gcttatatga actagggaat aagcgacttg ctatatccga gctcgaaaat ttattacata
     2161 atacagatcc attcacggac aaagaacaag cacagaaatt tttgcataaa attaagagcc
     2221 aacaaggatg attgtgtgat gacacatttt cgctataaaa aaatttgttt gcttgtgtct
     2281 attgcactgt ttttagtagg gtgttcgcat aactatccct tattagaaaa gggagcctat
     2341 acttacaatc ttcattcgtc ttaccacata ggcccaggag atcatctttc cattctcgta
     2401 tggaaaaatg cagatcttac cctgggtgtg attgtccggc ccgatggttt tattacactt
     2461 cctttggtcg atgatattca ggcagcaggc aaaaccccca tggttttagc ggaagattta
     2521 gaacacgcgc tggcagagta tcttaaagat cctttagtaa cggtgatggt tacaggattt
     2581 ataggaacct atacggatac tattcgaatt gttggtgaag caactcagcc tcaatctctt
     2641 ccttatagag atggtatgac ggttctagat ctgatgattg tagttgga
//
LOCUS       DASTIB010000050         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_875904, whole genome shotgun sequence.
ACCESSION   DASTIB010000050 DASTIB010000000
VERSION     DASTIB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875904"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFP93_00840"
     CDS             <1..577
                     /locus_tag="VFP93_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET9843185.1"
                     /translation="NILVPHEYIGNVIQLCIERRGTQTKMHYVGKQVELSYEMPLSEV
                     VLDFFDRLKSVSRGYASLGYTFKRFQEADLVKMDILINGEPVDALALIVHRSNSFYRG
                     REITNKMKELIPRQMFEVAIQAAIGGQIIARTTVKALRKNVTAKCYGGDVSRKRKLLE
                     KQKAGKKRMKQVGKVEIPQEAFLAILQVDKD"
     gene            583..1494
                     /gene="lepB"
                     /locus_tag="VFP93_00845"
     CDS             583..1494
                     /gene="lepB"
                     /locus_tag="VFP93_00845"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9843186.1"
                     /translation="MKLYFDFSAILGIATLITGMLWAIDSLFLKSKRLRKNQAVLDKL
                     GLAATDVSTIPPEEIDEKIKQQGEIHETAAKMPKWADYSRSFFPVLLLVFVLRAFIIE
                     PFRIPSGSMRPTLQEGDFILVNKFAYGLRLPLTGTKLLDWGKPQRGDILVFRYPHDTS
                     VDYIKRIIGLPGDKVKVVNKQLYVNGEPVSQEYIETNFETDDGGLRKVKRYHEQLDST
                     VYSIYSSLIQRPDFAEIEVPANHFFVLGDNRERSRDSREWGFVPEHLILGKAFFIWLS
                     FDAQKKDIRWERFGSIQSSQREQQPHA"
     gene            1487..1861
                     /locus_tag="VFP93_00850"
     CDS             1487..1861
                     /locus_tag="VFP93_00850"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HET9843187.1"
                     /translation="MPKKIHRQKGMTFVSWLVIALVGITILILGIRLVPAYLESFAVK
                     KSLESLAEEKGILKKSPASVKDLLSRRFQVNEVRHVDPKALKIEKVGDQHQLRLDYEI
                     RVRALGNIDAVLRFDQKVTVEQ"
     gene            1858..>2280
                     /locus_tag="VFP93_00855"
     CDS             1858..>2280
                     /locus_tag="VFP93_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HET9843188.1"
                     /translation="MRNLRSLENLLGYTFKDVTLLQCALKHRSLGKPNNERLEFLGDS
                     LLNFIIANRLFEEFPTATEGELTRFRATLVNRQSLAEIASTLDLPNRIELGIGEKKSG
                     GFKRESILADALEAIFAAIYLDATELQPLIRVIDILYKN"
BASE COUNT          710 a          381 c          511 g          678 t
ORIGIN      
        1 caatatattg gtaccccatg aatatattgg caatgttatt caattgtgca ttgagcgcag
       61 gggtacacaa acaaaaatgc attatgtggg caagcaggtt gagctttcct atgaaatgcc
      121 gctcagtgag gtagttttag atttcttcga tagactgaaa tctgtgtccc gcggatacgc
      181 ttctttaggt tataccttta aaagatttca agaagcagat ctagtaaaaa tggacatttt
      241 aattaatggc gaaccagtgg atgctctggc acttattgta catcgaagca attctttcta
      301 tcgtggacgt gaaataacca ataaaatgaa agaactcatc ccaaggcaaa tgtttgaagt
      361 agcaattcaa gcagctattg gaggacaaat tattgcgcgc acgaccgtaa aagctttgag
      421 aaaaaatgtt acggctaaat gttatggagg agacgtctct agaaagcgta aacttctaga
      481 aaagcaaaaa gcaggaaaaa aacgtatgaa acaagtaggg aaagtagaaa tccctcaaga
      541 agcattttta gcaattttgc aggtggataa ggattgattt ccatgaaatt gtattttgat
      601 ttttcagcga ttttaggaat agcaacatta ataacgggca tgttatgggc tattgattct
      661 ctatttttaa aaagtaagcg tttgcgtaaa aatcaagcag tgcttgataa actgggttta
      721 gcagcgaccg atgtgagcac aatcccccca gaagagatag atgaaaaaat caaacaacag
      781 ggggaaatcc acgaaaccgc agcaaaaatg ccaaaatggg cggattattc acgttctttt
      841 tttcccgttt tgttattagt ttttgttttg cgggctttta ttattgaacc ttttcgaatt
      901 cctagtgggt ccatgagacc aacactgcag gaaggagatt ttattctagt caataagttt
      961 gcctacggtt tacgtttgcc attaacaggt acaaagctcc ttgattgggg taaacctcaa
     1021 agaggtgata ttttagtttt tcgctatccc catgatacca gcgtcgatta tatcaaacgt
     1081 ataattggtt tacctgggga taaggtgaag gtcgttaaca agcaattgta tgtcaacggt
     1141 gaacccgttt cgcaagagta tatcgaaacg aattttgaga cggatgatgg ggggttaagg
     1201 aaagttaaac ggtatcacga gcaattggat tctacggtat attccattta tagtagttta
     1261 attcaacgtc ctgactttgc cgaaatagaa gttccagcaa accatttttt tgtgttgggc
     1321 gataatcggg aacgtagtcg agatagtaga gaatggggct ttgtgccaga gcatctgatt
     1381 ttagggaagg cattttttat ttggttaagc tttgatgcac agaaaaagga tattcgttgg
     1441 gagcgtttcg gttccattca atccagccaa agggagcaac aaccgcatgc ctaaaaaaat
     1501 tcatcgccaa aagggaatga ctttcgtttc ttggttagtg atagccttgg tgggtattac
     1561 catactcatt ttaggtattc gcttagtacc ggcttattta gaaagttttg cggtgaaaaa
     1621 atctttagaa tctttagctg aggaaaaagg tattttaaaa aaatctccgg cttcggtaaa
     1681 agatcttttg agccgtcgct ttcaagtgaa tgaggtgcgc catgtggatc ccaaagcatt
     1741 gaaaatcgaa aaagtagggg atcagcacca attgcgtttg gattatgaaa tacgtgtacg
     1801 tgctttgggc aatatcgatg ctgtgttacg ttttgaccaa aaagtcacgg tagagcaatg
     1861 aggaatttgc gttctttaga aaatttacta ggatacacgt ttaaagatgt aacgttgttg
     1921 caatgcgcgt taaaacaccg ctctttaggc aaaccgaata acgaacggtt agaattttta
     1981 ggcgattcct tattaaattt tattattgcg aatagacttt ttgaagaatt tcccactgcc
     2041 accgagggag agctaacccg ttttcgtgca acgcttgtta accgccaatc cttagcagaa
     2101 atagcatcga ctttagactt accgaatcgt attgaactag ggattggcga aaaaaaaagt
     2161 ggaggtttta aacgagagtc tatcttagca gacgccttag aggctatatt tgctgctatt
     2221 tatctagatg cgactgaatt acaaccctta atacgggtta ttgatattct gtataagaat
//
LOCUS       DASTIB010000051         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1367773, whole genome shotgun sequence.
ACCESSION   DASTIB010000051 DASTIB010000000
VERSION     DASTIB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367773"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VFP93_00860"
     CDS             complement(<1..867)
                     /locus_tag="VFP93_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9843189.1"
                     /translation="METPTNDSQMIQKHLLNLVHTFLLSLEKKRAADILNLDSKISSE
                     LGIDSLGRVELFHQIEDKFGIYLPENLLAEAETIRDIFNRIEQEDYSKDLIIGTQELH
                     FTETTINPLHFKTLVEVLLKRGAKEPTRVHIYYQDEKGHSHPITYGELLKSSLRVASN
                     LQAWGFRKGETVAIMLPTCPEFFYTFMGIMLAGLIPVPIYPPFRPDKLEEYALRESII
                     LKNAEVRLMVTFYAAEKLASLLKVFIPNLKKVVTAHALLQGEEKFAHVALKGSSPAFI
                     QYTSGSTSDPKGV"
     gene            1031..1762
                     /locus_tag="VFP93_00865"
     CDS             1031..1762
                     /locus_tag="VFP93_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HET9843190.1"
                     /translation="MQDVHKTEIWLDTLNTEALDHAHELGILQGITTNPKIMAMECSH
                     PLDALNHLLETYACTVAVQVTEQEAAACIREAQALYRYNQRILIKIPCIQSGYEAMAE
                     LKQSNIPFLATAIYHPRQFYIANQLGARYAAFYYFRMIDFYENYYKLIKKDAKTKVIE
                     DLKKIMLIAKNSQTKLMAANLKSVEDIEFILDHRMDSITIPRDPYLHFFKDFEVAKEA
                     TESFLNLWQKTFSTKTWDQFMQVEV"
     gene            complement(1743..2510)
                     /locus_tag="VFP93_00870"
     CDS             complement(1743..2510)
                     /locus_tag="VFP93_00870"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET9843191.1"
                     /translation="MAIWHEIQGVDYLCRHETFVWRIVEDQSKSTTRKFVDSLEEHEV
                     LENLIEASKPKLTITPKESSDLHYLLSTPFRYPPLRWGSRFGTRFDRGILYGSLDLPT
                     AMCEKAYHRLAFLTASEGDLGGKTTYYTSFQFKAQSNTFVNLIQNPFNTYQDLITSKK
                     SYDFTQALGSQMRTAQVDFFLYPSARTKELGQNIGIFSPKAILHNRQISRTFQSLHCY
                     ATKEMVEFSLTHRKEKIHQFPIADFLVDGVIPIPPLA"
     gene            complement(2513..2878)
                     /locus_tag="VFP93_00875"
     CDS             complement(2513..2878)
                     /locus_tag="VFP93_00875"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HET9843192.1"
                     /translation="MKLQKSVDANFVLNKALVKASQYIGLTQREVSAIVGSSPAQISR
                     LFKYATPCIHKDTKEGECALLFLRLIRSLEALVGEDPKEAHTWLYSYNHHLRGIPIDL
                     IKTIVGLHEVVLYLDAMRG"
BASE COUNT          967 a          560 c          535 g          976 t
ORIGIN      
        1 aacgcctttt ggatcactgg tgctacccga agtatattga ataaacgccg gagaggaacc
       61 tttcagcgcg acatgggcga atttctcttc cccttgtagt aaggcgtgtg cggtgacaac
      121 tttttttaaa ttcggaataa acacttttaa taagcttgcc aatttttccg ctgcataaaa
      181 agtaaccatt aaacgcactt cagcattttt taaaattata gattctctta atgcgtattc
      241 ttctaattta tcaggacgaa aaggcggata tataggaaca ggaattaaac ctgccaacat
      301 aattcccata aaagtataga aaaattctgg gcaggtgggt agcataatag ccacagtctc
      361 accctttctg aatccccaag cttgtaaatt ggaggcgact ctaagactac tttttaataa
      421 ttctccatac gttataggat gcgagtgtcc tttctcatct tgatagtaaa tgtggacgcg
      481 tgtcggttct ttagcgcccc gtttgagtaa aacctctact aaggttttaa aatgtaaagg
      541 attaatcgta gtttcagtaa agtgcagctc ttgggtacct ataattaaat ctttgctata
      601 atcctcttgt tcaatgcgat taaaaatatc acgaatcgtt tcagcttctg ccaataaatt
      661 ttccggaaga taaatcccaa atttatcttc aatttgatga aacaattcta cacgtcccaa
      721 actatcaatg cctaattcgg aagaaatttt cgaatctaga tttaaaatat cggcagctct
      781 ttttttttcc agcgagagca aaaatgtatg tactaaattg agcaaatgtt tttgaatcat
      841 ttgcgaatcg ttggtaggtg tttccatgct tcaaagctta ggacaaataa atgtccacat
      901 tataggggga atctgtaagt aaaaaaacct aaaaaaattt aaaattggca agaaagctaa
      961 atataaaaat atttcctatt ttatctttcc ttaacacgga tataatagtt acccgaattc
     1021 cggaggttct atgcaagatg ttcataaaac agaaatatgg ttagacactt taaatacaga
     1081 agcactagat catgcacacg aattaggtat tttacagggt ataactacta atcccaaaat
     1141 tatggccatg gaatgctctc accctctgga cgctttgaat catttacttg aaacctatgc
     1201 ctgtactgtt gcagtgcaag ttacagagca agaagctgca gcatgcatta gagaagcgca
     1261 agcgttatac cgctataatc agcgcatatt aattaaaatt ccttgtattc aatcaggcta
     1321 tgaagctatg gctgaattaa aacaatccaa tattcctttt ttagcaaccg ctatttacca
     1381 tccacgccag ttctatattg ccaatcaatt gggtgctagg tatgctgctt tttattattt
     1441 tcgtatgata gatttctatg aaaattatta taaattaatt aaaaaagatg ccaaaacaaa
     1501 agttatagaa gatttaaaaa aaataatgct tattgctaaa aattcgcaaa caaaacttat
     1561 ggcagcgaat ttaaaaagcg tggaagacat tgaatttatt ctggatcatc gcatggattc
     1621 tatcaccatt cctcgtgatc cgtacctcca ttttttcaaa gattttgaag ttgcgaaaga
     1681 agctacggaa agttttttaa atctttggca aaaaactttc agcacaaaaa cttgggatca
     1741 gtttatgcaa gtggaggtat aggaattact ccatcgacta aaaaatctgc aattggaaat
     1801 tgatgaattt tttctttgcg atgggttaac gaaaattcta ccatttcttt ggttgcgtaa
     1861 cagtgtaaac tttgaaatgt tctggaaatt tgacggttat gtaaaatggc tttcggggaa
     1921 aaaatcccta tattttgacc taattctttt gttcgtgctg aaggataaag aaaaaagtcc
     1981 acttgcgcgg tacgcatttg gctcccaaga gcctgtgtga aatcataact tttttttgaa
     2041 gtaatcaagt cttgataggt attaaaagga ttttgtatta aatttacaaa tgtattactt
     2101 tgtgctttaa attgaaaact cgtgtaatac gtggtttttc ctcctaaatc gccttcactt
     2161 gcggttaaaa atgctaaacg atggtaagct ttttcgcaca tagccgtggg taaatctaat
     2221 gagccgtaca gtattccgcg atcgaatctc gtgccaaaac gtgagcccca gcgtaaaggt
     2281 ggatatctaa atggtgtact gagtaaataa tggagatccg aactttcctt aggggtaatt
     2341 gttaattttg gtttgcttgc ttcaattaaa ttttctaata cctcgtgctc ttctaacgaa
     2401 tcgacaaatt tacgtgtggt agatttagat tgatcttcaa cgatacgcca aacaaacgtc
     2461 tcatgcctac aaagataatc cactccttgt atttcatgcc aaattgccaa ttttaacctc
     2521 gcatagcatc taaatacaaa acaacctcat gcaaacccac gatggttttt attagatcga
     2581 tcgggatccc gcgtagatgg tgattatagc tatagagcca agtgtgagct tctttcgggt
     2641 cttcgcccac aagggcttcc aaacttcgaa ttaagcgtaa aaaaagtaaa gcacactccc
     2701 cctctttcgt atccttatga atacagggcg ttgcatattt aaaaagtctt gatatttgag
     2761 cagggctaga gcctacgatc gcagacactt ccctttgtgt gagtcctata tattggcttg
     2821 ctttaaccaa cgctttattc agcacaaaat ttgcatctac acttttttga agcttcatat
     2881 ttaccttcaa atattccata tgaaatatta tagcataata atttcacctg caatttaaat
     2941 cttacttaaa aggcgctctt ttttgtatgg gtgttctgtt gaaaatctga catacgtcaa
     3001 tatttcaaac tgtgataatg gtataaatta tggactcg
//
LOCUS       DASTIB010000052         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_875721, whole genome shotgun sequence.
ACCESSION   DASTIB010000052 DASTIB010000000
VERSION     DASTIB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875721"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..630
                     /locus_tag="VFP93_00880"
     CDS             106..630
                     /locus_tag="VFP93_00880"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HET9843193.1"
                     /translation="MHSVHDKQRKLGILLIFAAWGILLGLLTVFFHNWYSFKQNPNQH
                     ITSLEMPDGSRQIILQANSQGHYLAVGTLEDQEVTFLLDTGASTVVVPLKLANKIPLA
                     MGPKMLAHTAGGEVSIISTRIKKIQIGNIVLKNIPASISDRLPEGYVLLGMSALRRLK
                     LEHAHDTLIITLPP"
     gene            complement(614..1159)
                     /locus_tag="VFP93_00885"
     CDS             complement(614..1159)
                     /locus_tag="VFP93_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843194.1"
                     /translation="MKLLCSKFLILFFLLFNGSLFAQESSQEVPVKMRLGFIKIHKSS
                     EKRGDELYFSVTEYPSHERPKNYLIPTFPSHWLSQHLANVRDVELWSRTLKNNESVQV
                     VISLMERDAPPWNIDDLIGTIKFKAKNSNGQIKKAWSIPNQKDQETHTDNTNQFKFYG
                     ENSEYQLELILEDSVKIKAAT"
     gene            1320..>2133
                     /locus_tag="VFP93_00890"
     CDS             1320..>2133
                     /locus_tag="VFP93_00890"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET9843195.1"
                     /translation="MLKQLPSPIKGILNAFALVIHTFFWGTLLFCFFLVKIAMPLKAW
                     HHYWSAQCIKVGEWWTATCMFMQKLTTNFTPRVEGLEALNKHSWYFIAANHQSWTDIF
                     LLLGLFNKKIPFIKFFIKDQLKYFPILGFAWWAFEYPRMKRYSEKTLQSKPQLRGKDL
                     ETTLKQCNKFLQKPTTLLSFIEGTRFTPAKHKKQNSPFKHLLRPKAGGFALTIHALRD
                     RIDHVLHVTIAYPKDKKNFWHFLCGHINTPYIKIEKIKIPDYLFSGDYFNDPL"
BASE COUNT          681 a          407 c          362 g          683 t
ORIGIN      
        1 atggtaagat ctcgcataat catgctgttc atataatgca tcacctactt actatttaga
       61 aaacaaactg ctatttttaa taaagaatac atttcttgtt agactatgca ctcagtccac
      121 gataaacaac gtaagcttgg aatcctatta atttttgcgg cttggggcat tttattaggg
      181 cttttaactg ttttttttca caattggtat tcttttaagc aaaaccccaa ccaacatata
      241 accagcttag agatgcctga cggaagtagg caaattattt tacaagcaaa cagtcaaggc
      301 cactacttag cagtaggtac tttagaagat caagaagtaa cttttttgct cgatacgggg
      361 gcttctaccg tagtagtccc tttaaaactt gccaacaaaa ttcctttagc tatgggacct
      421 aaaatgctag cacacaccgc aggaggagaa gtgtcgatta tatcaacccg tatcaaaaaa
      481 attcaaatcg gtaatattgt tttaaaaaat attcccgcaa gcataagcga tagacttcca
      541 gaaggttatg tgctgttagg tatgagtgcg ttgcgaagat taaagctcga gcacgcgcac
      601 gatacgttaa ttattacgtt gccgccttaa tttttactga atcttccaaa attaattcta
      661 gttgatattc agaattttcc ccataaaatt taaattgatt ggtattgtct gtatgcgttt
      721 cctggtcttt ttggttggga atactccacg cttttttgat ttgaccgttg ctatttttag
      781 ctttgaattt tatggttcct attaaatcat caatattcca aggcggagca tcgcgttcca
      841 ttagggaaat aaccacttga accgattcat tattttttaa agttcgactc caaagctcta
      901 catcgcgtac attagcaaga tgttgcgaca accaatgaga ggggaatgta ggtattagat
      961 agtttttagg acgctcatgc gaaggatact ctgttacact aaaatagagc tcatcacctc
     1021 ttttctctga agatttgtga attttaataa atcctaatcg cattttaacg ggaacttcct
     1081 gactggattc ttgtgcaaaa agtgaaccgt tgaacaatag aaaaaatagg atcaagaatt
     1141 ttgagcaaag taacttcata actatccttg ttactgcaac accctcttac tttatgatga
     1201 agcttacata atcgtcaagc aaccatcttt ttaaaattta agaatgctta ctaaaacctg
     1261 atattgcatt aaaggatttt cataaatact atatttgcag agctccaaaa acaatgaata
     1321 tgctaaaaca attgccctcg cctatcaaag gtatattaaa tgcatttgcc ctagttatac
     1381 atacattttt ctggggcacc cttttgtttt gcttctttct tgtaaaaata gcaatgccgt
     1441 tgaaagcttg gcaccattat tggagtgctc aatgtattaa ggttggagag tggtggacag
     1501 caacttgcat gttcatgcag aaactcacaa ccaactttac gcctagggtt gagggtctag
     1561 aggctcttaa taaacatagt tggtacttca ttgccgctaa tcaccaaagc tggaccgaca
     1621 tttttcttct actgggtctt tttaataaaa aaattccttt tataaaattt tttataaaag
     1681 atcaattaaa atattttcct atccttggct ttgcctggtg ggcctttgag tatccacgca
     1741 tgaagcgtta tagtgaaaaa acattacaaa gcaaaccaca attacgagga aaagacttag
     1801 agaccacatt aaaacaatgc aataaatttt tacaaaagcc aaccactttg ttaagtttta
     1861 tagaaggtac gcgcttcact cctgctaaac ataaaaaaca aaattcccct ttcaaacatt
     1921 tattacgccc caaagctggg gggtttgctt taaccataca cgcgttacgt gatcgtattg
     1981 atcatgtatt gcacgtgacc atagcgtatc caaaggataa aaaaaatttt tggcactttc
     2041 tttgcgggca cataaatacc ccctatataa aaatagaaaa gataaaaatc cctgattatt
     2101 tattctctgg cgattatttc aacgatcctt tgt
//
LOCUS       DASTIB010000053         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_770892, whole genome shotgun sequence.
ACCESSION   DASTIB010000053 DASTIB010000000
VERSION     DASTIB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770892"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2227
                     /locus_tag="VFP93_00895"
     CDS             <1..2227
                     /locus_tag="VFP93_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843196.1"
                     /translation="ESVPLELEEPKDNSKFSTACSAWCLDDQMHGEIPVESRKWMVSQ
                     GNSILQKLYGLENLDFNNIEKLLKDLGMKDLVKNSGIMELQLYEKICHRSPITKWFSP
                     KILWKVANQNRKNESAKEQMRKEFLLGIVCVMWALYNQSAKQGDFFERGSFKIIDAQD
                     KLFHLLKKYVQYASGQDNPTYANGSDFAYSRDPKRKKSSHYKDPEFKSEQYGVDMRLW
                     HNFPNVKILPHQMAHLLCGQIEVNEKKFTFVKFESIGLGNMLEFMEHGMNFLHPRKKA
                     AIYRREKDIPQNLQDIYTQFQKKYYKLTGEKLHAKNVNAMYKQIFEARKLGNSFNNLF
                     LDFSKVNHVEIRTGNEVILDFATSFLSTQELDTIEKEYSNYVNQFHPNATIFEKNVQE
                     TKAIHPNKFDTTTSLADDLENISLADFIKKAHSASRDQLVIDVIHEYANENTNADPSI
                     KKFIDSFAALNVLRTLQVTYDTKLRELRNLKDPQAEQLTQPLLQSYSEYKYKLLQALM
                     ETQEPSLVNLVATVDRFAPKLEIRENADQNVGLSNNGEMRKSKSAFAPLHKTTQQEET
                     GQSRVKRQFRVPEVEITNLLNAFGSNQGKIEQNLKKLYQDTKKGVKGRLKSFYTRFGH
                     TSANSDYQIKGLAILIADLQKEPRFELREKGIIMQAYLLIWREESKKLGSPWQKILQN
                     LEQKIHAITNISLNRDEAINIINAHQSFKSSGEMQQFNQLLDQMIPNPPNFKPHNKKP
                     S"
     gene            complement(2259..2549)
                     /gene="minE"
                     /locus_tag="VFP93_00900"
     CDS             complement(2259..2549)
                     /gene="minE"
                     /locus_tag="VFP93_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HET9843197.1"
                     /translation="MKSFDFKFFDFLKIPKRTPAKVAKERLQIIVSHESSRRSKDFIK
                     DLQRELLEVISKYVNIDHEQIKVQLEQHGGQSVLELNVTLQEKNKTAEVEST"
     gene            complement(2549..>3221)
                     /gene="minD"
                     /locus_tag="VFP93_00905"
     CDS             complement(2549..>3221)
                     /gene="minD"
                     /locus_tag="VFP93_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HET9843198.1"
                     /translation="RVVFDFINVINGDANLKQALIKDRRQENLFILPASQTRDKDALT
                     HEGVEKILNELKEEFEFIICDSPAGIEKGAMMALYFADEAIVVTNPEVSSVRDSDRIL
                     GVLSSKSSRAEKQLDPIKEHLLITRYSKSRVDKGEMLSIQDVCEILSIPLLGVVPEST
                     DVLAASNRGVPVTTDDSNTSNGDARQAYEDAVSRLLGEKIPITREESWTRKLMKKLNL
                     FRSPA"
BASE COUNT         1112 a          587 c          585 g          937 t
ORIGIN      
        1 agagagtgtc cccttagaat tggaagaacc gaaagataac tcaaaatttt ctactgcgtg
       61 tagcgcatgg tgtcttgatg atcaaatgca cggagaaatt ccagttgaat cgcgcaagtg
      121 gatggtaagt caagggaata gcattttaca aaaattgtat ggactggaaa acctcgattt
      181 caacaatatt gagaagctat taaaagattt aggaatgaag gatttggtaa aaaatagtgg
      241 aattatggaa ttgcaattat atgagaaaat ctgtcatcga agtcctatta ctaagtggtt
      301 ttccccaaaa atattatgga aagtagcaaa tcaaaaccgt aaaaatgaat cggcgaaaga
      361 gcaaatgcga aaggaatttt tactgggaat tgtgtgtgtg atgtgggcgc tttataatca
      421 aagcgcaaaa caaggagatt tttttgaacg cggttctttt aagattattg atgcgcaaga
      481 caagttattt catctcttaa aaaaatatgt acaatatgca tccggacaag ataatccaac
      541 ttacgccaat ggcagtgatt ttgcttatag tcgtgatcct aaaagaaaaa aatctagtca
      601 ttacaaagat ccagaattta aatcagaaca atatggcgtc gatatgcggc tttggcacaa
      661 tttccctaac gtcaaaattt taccccatca aatggcgcat ttactttgtg gtcaaataga
      721 agttaatgaa aaaaaattta cttttgtaaa atttgaaagc attggtcttg gtaacatgct
      781 tgagttcatg gaacatggaa tgaactttct tcacccccgt aaaaaggctg caatatatcg
      841 acgtgaaaag gatattccac aaaatttgca agatatttat acacaatttc aaaaaaaata
      901 ttataaactt acgggcgaaa aactgcatgc aaaaaatgtt aatgcaatgt acaaacaaat
      961 tttcgaagcc cgtaagctgg gaaacagttt taataatcta ttcctcgatt ttagcaaggt
     1021 aaaccatgtt gaaattcgaa cgggcaatga agttattttg gactttgcta cttctttttt
     1081 aagtacgcaa gaattggata caatcgaaaa agaatattca aattatgtaa atcaatttca
     1141 tccaaatgcc acaatttttg aaaaaaatgt gcaagaaacg aaagcaattc atccaaacaa
     1201 atttgatact actacgtctt tagccgatga tttagaaaac atatctttag cagattttat
     1261 aaaaaaagca cattcagctt ctcgcgatca actagtgatt gatgtaatcc atgagtacgc
     1321 aaatgagaat acaaacgcag atccatctat aaagaaattt attgacagtt ttgcggcact
     1381 taacgtattg cgcactttgc aagttactta tgacacaaaa ctacgtgagt taagaaattt
     1441 aaaagatcca caagctgagc aacttacgca acccctttta caaagctata gcgaatataa
     1501 atacaaatta ttgcaagctt tgatggaaac ccaagaacct tcccttgtaa atttagtggc
     1561 cacggtggac cgtttcgcgc ctaagcttga aataagagag aatgcagatc aaaacgttgg
     1621 tttaagcaat aatggagaaa tgcgtaaatc caaatctgct tttgcaccac ttcacaaaac
     1681 aacacaacaa gaagaaactg gccaatctcg agttaagcgt caatttcgtg tacccgaagt
     1741 tgaaataacg aatcttttaa acgcatttgg atccaatcaa ggcaagatcg aacaaaactt
     1801 aaaaaaatta tatcaagata cgaaaaaggg cgtaaaaggc agattaaaaa gtttttatac
     1861 gcgttttggc catacatcgg caaatagtga ttatcaaata aaaggtttag caattcttat
     1921 tgcagactta caaaaggaac cgcgtttcga gcttagggaa aaggggataa taatgcaagc
     1981 ctatttgttg atatggcgtg aagaatctaa aaaattggga tcaccttggc agaaaatact
     2041 tcaaaacttg gaacaaaaaa ttcatgctat aacaaatata tcactgaatc gtgatgaagc
     2101 aatcaatatc attaacgcgc accagtcttt caagagcagc ggagaaatgc aacagtttaa
     2161 ccagctctta gatcaaatga ttccaaatcc acccaatttt aaaccgcata ataaaaaacc
     2221 cagctaaatg ctgggttaaa aatagcacgc gttagttact aagtgctttc aacttctgct
     2281 gttttgtttt tttcttgcaa agtcacattc aattccaaca ccgattgacc accatgttgt
     2341 tccaattgaa ccttgatttg ctcatgatca atatttacat atttactgat gacttccagc
     2401 aattcccttt gtagatcttt aataaaatct ttgcttcgac ggctgctctc atgcgaaaca
     2461 ataatttgta aacgctcttt cgctacttta gcgggagtgc gcttgggaat tttaaggaaa
     2521 tcaaagaatt tgaagtcaaa ggatttcatc atgctgggct cctgaataga ttaagtttct
     2581 tcatgagctt acgagtccaa ctctcttcgc gtgttatcgg aattttttct ccgagtagac
     2641 gactcaccgc atcttcataa gcttgacggg catcaccgtt gctagtattc gaatcatcgg
     2701 ttgttacagg aacaccacga ttggatgctg ctaaaacgtc cgttgattct ggcaccacac
     2761 ccaataaggg aatagataat atttcgcata cgtcttgtat actgagcatt tcacctttat
     2821 cgacacgact tttagagtaa cgcgtaatca ataaatgctc ttttatggga tcaagttgtt
     2881 tttcggcgcg agacgattta cttgagagta ccccaagaat tctgtcggaa tctcttacgg
     2941 atgaaacttc cggattggtg acgacaatcg cttcatctgc aaaatatagg gccatcattg
     3001 cccctttttc aattccagcg ggcgaatcac aaatgataaa ctcaaattct tcttttaatt
     3061 cattgagaat tttttctacc ccttcatggg tcaaagcatc tttatcacgt gtttgtgaag
     3121 ctggtaggat gaataaattt tcttgtctgc gatctttaat aagggcttgc tttaagttag
     3181 catcaccgtt aataacgttg ataaagtcaa acacaaccct a
//
LOCUS       DASTIB010000054         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_413376, whole genome shotgun sequence.
ACCESSION   DASTIB010000054 DASTIB010000000
VERSION     DASTIB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413376"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFP93_00910"
     CDS             complement(<1..338)
                     /locus_tag="VFP93_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET9843199.1"
                     /translation="MLYLKSFHIIFLVAWFGGLFYLPRLFVYHTEIEPTDPNYQRFCT
                     MERKLFWGIMTPCAILALIMGLGLLHYWWGAIQDKGWMHAKLLLVGFLICYHYQCYRY
                     LKAFKEKRNT"
     gene            454..810
                     /gene="erpA"
                     /locus_tag="VFP93_00915"
     CDS             454..810
                     /gene="erpA"
                     /locus_tag="VFP93_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET9843200.1"
                     /translation="MRVDTPISLTESAARRLLRLIQIENDPNLKFRVYITGGGCSGFQ
                     YGFAFEKENQPDDIIVDTYLGAEEIPLQIVIDPMSMTYLDGAVVEYQEGLYGSHFTIK
                     NPNAKNTCGCGNSFSL"
     gene            complement(807..1940)
                     /locus_tag="VFP93_00920"
     CDS             complement(807..1940)
                     /locus_tag="VFP93_00920"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET9843201.1"
                     /translation="MRRSEIYIGALSGTSVDSLDIAAWDFSTPEKIALLASSKVLIES
                     SLKNHILTLSTSGTCHFKEFGQLDKQLGLLFADGVVDLLEKAALSPKDIIAIGSHGQT
                     VWHEPNLLFPFSIQIGDPNVIAQKTGIVTVADFRRADLALGGQGAPLVPAFHHWLFHT
                     KLQNRIILNLGGISNISVLFSDHAKSLLGYDTGPGNALLDAWTQYHLGLPFDQDGEFA
                     KKGTTNHLLLKKLLADSYFKKPYPKSTGKDTFNLAWLSKFDVAHLNPEDVQATLVSLT
                     AISIREEIMKHVDSGAVYTCGGGSLNPVLMQAIRQALGNAFKVQTTSLLNLDPEFVES
                     ALFAWLARERLNQRPITLSTVTGCTHDTLLGAIYAPKNITSVC"
     gene            complement(1937..3316)
                     /locus_tag="VFP93_00925"
     CDS             complement(1937..3316)
                     /locus_tag="VFP93_00925"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9843202.1"
                     /translation="MKQIDRVKMDYKPKPPQDRALSFRPYSKVFFKCSLTFVIFIALL
                     NFVSLARGSYKNFTKNSFNNFLTEEIKLPAVNLFTVRKAKSLEPIVDASWSTLKVRSG
                     DNLGLIFKKLGLPSNELNNILSIGKDAKFLKELRPGQTIKVLLDKKGHLLAMQQKIDP
                     MKTLTIERTNGKFTGLLQEALIEQRIAFGKAHIQDSLFGAGKKSNLEVKLIMELADIF
                     AYDIDFALDIQPNDSFKVLYEEKFADGKKVGVGNILAAEFTNQGKTYRAIRYVSQGKA
                     EYFSPNGRGLKKAFIRTPVNYTHISSYFNLQRKHPVLHKIRAHRGVDYAAPTGTPVKA
                     AGNGQVIFLGRKGGYGNTIILQHGERYTTLYAHLSSFKKLLRVGSKVKQGDIIAYVGS
                     SGLATGPHLHYEFRVDGIHRDPLTVALPNSDGMQGKQRHAFLAHADRLIDLMNHHDKV
                     MLASSEFAQ"
BASE COUNT         1135 a          690 c          689 g         1099 t
ORIGIN      
        1 ttcgtatttc ttttttcttt gaaggctttt aagtatcgat agcattggta gtgataacaa
       61 atcagaaatc ctaccagtaa taatttagca tgcatccaac ctttgtcttg gatagcaccc
      121 caccaataat gtaataatcc caaacccata atcaaagcta aaattgcaca cggagtcata
      181 atgccccaaa ataatttgcg ctccatcgtg caaaaacgtt gataattggg atcggtaggc
      241 tcaatttcgg tgtgatagac aaaaagtcga ggcaagtaga ataatccgcc aaaccaggca
      301 actaaaaaaa taatatgaaa cgatttgaga taaagcatgt gctgacccta tcctagttgg
      361 gaaatattat agggttttaa tgcgtctatc gctatactaa tcccttataa taagataagg
      421 taacaaaaat tttgataact aaatggttca aatatgcgtg ttgacacacc tattagctta
      481 acagagagcg cagcccgccg acttttgcgc ctcatccaaa ttgagaatga tccgaattta
      541 aaatttcgtg tctatataac aggagggggc tgttctggat ttcaatatgg ctttgcattt
      601 gaaaaagaaa atcagccaga tgatattatt gttgatactt atctaggagc cgaagaaata
      661 cccttgcaaa ttgttattga tccgatgagt atgacgtatt tagatggggc ggtagttgaa
      721 tatcaagaag ggttatacgg ctcacatttc actataaaaa atcccaacgc aaaaaatacc
      781 tgtggttgtg gtaattcttt ttcgctttag cacacggatg ttatattttt cggtgcataa
      841 atagcgccta gcagtgtgtc gtgtgtgcaa cctgtcaccg tggataaagt aataggccgc
      901 tgattcagcc gttctcttgc cagccaagcg aataaagccg attccacaaa ttccggatct
      961 aggtttaata aactcgtggt ctgaacttta aaggcatttc caagtgcttg ccttatcgct
     1021 tgcattaata cgggatttaa acttccacct ccacaagtat atacagcccc actatccacg
     1081 tgtttcataa tttcttctcg aatcgaaatg gcagttaaag aaacgagggt tgcttgtacg
     1141 tcttcagggt ttaaatgtgc tacatcaaat tttgatagcc atgcaagatt aaaagtatcc
     1201 ttgcccgtac tttttggata gggcttttta aaataagaat ccgcaagcag cttttttagt
     1261 aaaagatgat tcgtagtccc ctttttggcg aattcaccat cttgatcaaa agggagtcca
     1321 agatggtatt gtgtccaagc atctaataga gcattcccgg gacctgtgtc ataacctaat
     1381 aaggattttg cgtgatcgct aaagaggacg gagatatttg aaattccacc caaatttaaa
     1441 atgatccgat tttgcaattt tgtgtgaaaa agccaatgat gaaaagcggg aaccaagggt
     1501 gcgccttgac ctcctaaagc caaatccgct cttcgaaagt ctgcaacggt gactatacct
     1561 gttttttggg caatcacatt gggatcacca atttgaattg aaaaagggaa tagaagattc
     1621 ggttcatgcc aaacagtttg accgtggcta ccaatagcaa tgatgtcttt tggggagagt
     1681 gcggcttttt ctaaaagatc aacgacacca tccgcaaata ataaacctaa ttgtttgtct
     1741 aattgaccaa attctttaaa atgacaagtg ccacttgtgc tcaatgttaa gatatgattt
     1801 ttgagcgatg attcgattaa aactttcgaa gacgcaagta aagcaatttt ttcaggtgtc
     1861 gaaaaatccc aagcagctat atctagacta tcaacgctag ttcccgagag cgcacctata
     1921 tatatttctg aacgtctcat tgtgcaaact cagaggatgc aagcatgact ttatcgtgat
     1981 gattcattaa atcaattaag cgatcggcat gcgctaagaa agcatgccgt tgtttacctt
     2041 gcatgccatc ggagtttgga agagctaccg tcagtggatc tcgatgtata ccgtcgacgc
     2101 ggaattcata atgcaaatgc ggacctgttg ctaaaccaga gctgcctaca taagcgataa
     2161 tatcaccttg ttttactttg gaacccacac gtaacaattt tttgaagctt gataaatgcg
     2221 cgtataaggt agtataccgt tcgccgtgtt gcagaataat ggtatttcca tatcctcctt
     2281 ttcgccctaa aaaaatcacc tgcccattcc cagcagcctt tactggtgtt ccagtaggcg
     2341 ctgcataatc tacacctcgg tgagcgcgaa tcttgtgcaa aactggatgc ttgcgttgta
     2401 ggttaaaata gctactaata tgggtataat tcacgggtgt tctaataaag gcctttttta
     2461 accctcttcc attaggggaa aaatattctg ctttaccttg tgatacatag cgaatagcac
     2521 gataggtctt tccttgattg gtaaattctg ctgccaatat attcccaacc cccacttttt
     2581 tgccgtcagc aaatttttct tcatataaaa ctttaaaaga atcgttaggt tgaatatcta
     2641 aggcaaaatc aatatcgtat gcaaatatgt ctgctaattc cataatgagt ttaacttcga
     2701 gatttgactt tttacctgcg ccaaataaag aatcttgaat gtgtgccttt ccaaaggcta
     2761 tgcgttgttc aatcagtgct tcttggagta atcctgtaaa cttgccattg gtacgctcaa
     2821 tggtaagagt cttcatagga tcaatctttt gctgcatagc taataaatga ccttttttat
     2881 ctaatagaac tttaatagtt tgacctgggc gaagttcttt caaaaatttg gcatctttgc
     2941 ctatagaaag aatgttattt aactcattcg atggtaagcc taatttctta aaaattaatc
     3001 ctaaattatc ccccgaacga acttttaaag tagaccaact tgcatcaact atgggctcaa
     3061 gagatttagc tttgcgaacc gtaaataaat ttaccgctgg taattttatt tcttctgtta
     3121 aaaaattatt aaaggagttc ttggtaaaat ttttgtaaga tccccgtgcc aaggacacaa
     3181 aatttaataa ggctataaaa attacgaaag ttaagctgca tttaaagaag accttactat
     3241 aagggcgaaa agaaagcgca cgatcctgag gtggcttggg cttataatcc atttttaccc
     3301 tatcgatttg tttcatgaat atttaggtat tctaactata aagctagcct tagtaagtga
     3361 taaaattaaa gtattcaata gctttccgta cctgaaaaaa agaaaaaaag attactgaat
     3421 aataagttaa caactttgaa agttagttaa cgccagtatt catgctcaaa taaaatatta
     3481 ttctatagga tgctgaacgt caaaatgaat gatttaaacg aagcgcttag cttaattact
     3541 cgcggggtgg atgagatcat accgcttgat gatcttaaaa ctagactgaa agaaaatcgc
     3601 cctttgcgag tga
//
LOCUS       DASTIB010000055         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1173424, whole genome shotgun sequence.
ACCESSION   DASTIB010000055 DASTIB010000000
VERSION     DASTIB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173424"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="VFP93_00930"
     CDS             <1..1803
                     /locus_tag="VFP93_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9843203.1"
                     /translation="ACHWCHVMAHESFEDQETAEYLNKHFICIKVDREERPDIDNLYM
                     RALMMFSGQGGWPLNLFLDLDGKPFFGGTYFPKQPVYGLPTFQNTLSEIVRIWESHQE
                     EVKQTAQHLQRRLNQKLLADPAKLLPDFHSIIERITLLEEYVDPQQGGLLGAPKFPQI
                     PLWSLLFMHSNLAKNDNLVKYCHLTAHKLCKGGIYDHVGGGFFRYSTDAFWFAPHFEK
                     MLYDNALILEWLSLLYAHKPDPIYHRAVAQTIQWLEKEMYIDGAFAASLDADSEGKEG
                     KYYTWTWEELSLCLGEDLALIRSFFGAREEGNWHGTNILHQNHEDEHSVTFNEEIEKI
                     LNKLLEVRKIRIRPERDEKILLSWNALLIVALCKASLFFNERSWLNLAISTYQTLLDK
                     LRHKSGWYHCYRANLIQKKAQIEDYAGIISAAIHLYQVTGNKNYLENAEQWYKETQTA
                     FLDVNNNLYNQNSESELIAQLQPLDDTAIPSGNGLMAINTIALGLLTGNPQYYENWTH
                     HMQTVLSHIEKQNPALYPCHFQSLLLYYRAIKLESSWTRKFPKFVETLGGNIPSHWLF
                     VNGIEYETIHCCDHKSCFAELTDVQDLRIKMHGR"
     gene            1960..>2795
                     /locus_tag="VFP93_00935"
     CDS             1960..>2795
                     /locus_tag="VFP93_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9843204.1"
                     /translation="MSSFSQFQLHENLMKALEDIGYEKPSPIQVETIPHILMGCDVIG
                     QAQTGTGKTAAFALPLLSKLDKNYSKTQILVLTPTRELTIQVSEALKKYAKYMKGVEV
                     LPIYGGQSYSTQIRALKYGATIVVGTPGRVMDHMRKGTLDLTNLRCMVLDEADEMLNM
                     GFLDDIQWIIEQCPKERQMVLFSATMPKPIQKIAQRYLKDPINVAIKSKTATASTINQ
                     RFIKLRSVHKLDALTRILESEVFDGLIIFTRTKQTTLEVAEKLKALNFKCAAINGDME
                     QK"
BASE COUNT          937 a          509 c          533 g          816 t
ORIGIN      
        1 gcatgccatt ggtgtcatgt tatggcacat gagagttttg aagatcaaga aactgcagag
       61 tatttaaata aacactttat ttgcataaaa gttgatcgag aagaacgccc ggatattgac
      121 aatctttata tgcgagcttt gatgatgttt tctggtcaag gtggttggcc acttaatctt
      181 tttttagatt tggacggaaa gccatttttt ggcggcacgt atttccccaa acaaccggtt
      241 tatggattac caacttttca aaatacattg tcagaaattg tacgtatttg ggaatcgcat
      301 caagaggaag ttaaacaaac ggcacaacat ttgcaacgta gactaaacca aaagctgttg
      361 gcagatcctg caaaattgct acccgacttc cattccataa tcgagagaat tacccttctt
      421 gaagagtatg tagatccaca acaaggtgga ctcttaggtg caccaaagtt tccacaaatt
      481 ccattatggt ctttactatt tatgcattct aaccttgcta aaaatgacaa cctcgtaaaa
      541 tactgccatt taactgcgca taagttatgt aaaggtggta tttatgatca tgtgggcggc
      601 ggtttttttc ggtacagcac cgatgcattt tggtttgctc cccactttga aaaaatgctc
      661 tatgataatg cgctcatcct tgaatggctt agccttttgt atgcgcacaa accagaccct
      721 atttatcacc gtgctgttgc acaaacaatt caatggcttg aaaaagaaat gtacattgac
      781 ggtgcttttg ccgcgtcttt agatgcggat agtgaaggta aagaaggaaa atactatacc
      841 tggacatggg aagaattaag cctttgttta ggagaagatt tagcattaat tcgctcattt
      901 tttggagccc gtgaagaagg taattggcat ggaaccaaca tattgcatca aaatcatgag
      961 gatgaacata gtgttacctt caatgaagaa atagaaaaaa tattaaataa gttgttagaa
     1021 gtgagaaaaa tacgtataag accagaacgt gatgagaaaa ttttactttc ttggaatgcg
     1081 ttgttaatcg tagcgctctg taaagctagt ttatttttta acgaacgtag ttggttaaat
     1141 ttggctatct cgacctatca aactttgtta gacaaactgc gtcacaaaag tggatggtac
     1201 cattgttata gggcaaatct tatccagaaa aaggcgcaaa ttgaagatta tgctggcata
     1261 atttcagcgg ctattcactt atatcaagta accggaaata aaaattattt agaaaatgct
     1321 gaacaatggt ataaagaaac acaaactgct tttttagatg tcaacaataa tttatataac
     1381 caaaatagcg agtcggagtt aatagcacaa ttacagccat tagatgacac tgctatccca
     1441 agcgggaatg gtttaatggc cataaatacg atagcacttg gactcttaac aggaaatccg
     1501 caatattatg aaaactggac gcatcatatg caaactgttc tgagtcacat tgaaaaacaa
     1561 aaccccgcgt tatatccatg ccactttcaa tctttattgt tgtattatcg cgctataaaa
     1621 ttggaaagca gttggactcg caagttccca aaattcgtag aaactttagg gggaaacatt
     1681 ccctcacatt ggctttttgt aaatggtata gagtatgaaa ccatccattg ttgcgatcac
     1741 aaatcgtgtt ttgctgagct gacagacgta caggatttac ggataaaaat gcatggaaga
     1801 tgaaaaagat catgaatttg cttagcttaa ctaactctct ttcaggtttt ttatcagaat
     1861 ctgtggcaat taatctaata aagtattaga atcccaactc actttatata aaaatgaaca
     1921 acatgaaagt tcttaaaatt atttatttca gaggtgccaa tgtcgtcttt tagtcagttc
     1981 caattacatg aaaatctaat gaaagcatta gaggacattg ggtacgaaaa gccttctcct
     2041 attcaagttg aaactatacc gcatattctc atgggttgcg atgtaatcgg acaggcacaa
     2101 acaggtaccg gcaaaacagc cgcctttgct ttacccttat tatcaaaact cgataaaaat
     2161 tattctaaaa cacaaatttt agtgttaacg cccacccgcg agcttaccat ccaagtttct
     2221 gaagcattaa aaaaatatgc caaatatatg aaaggggtgg aagtacttcc tatttatggt
     2281 gggcaaagtt attccaccca aattcgcgca ttaaaatatg gcgcaaccat tgtggttgga
     2341 acaccgggcc gcgtcatgga tcatatgcgc aaaggcactt tagatttaac gaacctaaga
     2401 tgcatggtgc tcgatgaagc ggacgaaatg cttaatatgg gatttttaga tgatattcaa
     2461 tggattattg agcaatgccc taaagaacgc caaatggttc tgttctcggc tactatgcca
     2521 aaacccattc aaaaaattgc tcagcgttat ttaaaagatc ctataaatgt tgcgatcaag
     2581 tcaaaaacgg ctacagcaag taccattaat caaagattta ttaaacttag atctgtgcac
     2641 aaattagatg cgctaacaag aattctagaa tccgaagtat ttgacggact tattattttt
     2701 acccgtacta aacaaacaac cttagaagtt gctgaaaaat taaaggcttt aaactttaaa
     2761 tgcgccgcta tcaacggcga tatggaacaa aagca
//
LOCUS       DASTIB010000056         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_726098, whole genome shotgun sequence.
ACCESSION   DASTIB010000056 DASTIB010000000
VERSION     DASTIB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726098"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFP93_00940"
     CDS             <1..500
                     /locus_tag="VFP93_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9843205.1"
                     /translation="LLGSREKIFLKYNIIPHAKPTFMAKSGFLFVGRLEQGKGIVTLL
                     ETWQHLKQNYILKIIGSGPLEDDLKRKFQKENIVFLGSLSSGSVQLHMANAKFLIQPS
                     LYYETFGLTILEAMAQGTPVIGFNIGTRPEFIEHNKNGFIVESEMLEQSLFEFENIPN
                     YHKLC"
     gene            587..1384
                     /locus_tag="VFP93_00945"
     CDS             587..1384
                     /locus_tag="VFP93_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9843206.1"
                     /translation="MLKHPKISVITVVKNSESTIEHTLKSVANQTYANKEHWILDGAS
                     TDNTLNIVKRYAQQYSHIQWFSAKDKGMYDALNKGMQLANGEILGFLHADDFYAKNSV
                     LDEVALSFNPFTQAVYSDLLYVDPHDLKKIKRYWQSKPYEKGAFARGWCPPHPTFFVK
                     KTTYERLGGFDTSLNLGNDVELMMRFMEKHNVPTKYCPGVWVVMRAGGISNKSIKNIL
                     QQNLSIMQSFKKHDLPFSWMYFILGKCIDRLKQIVHKHHVDYSLENA"
     gene            1381..2289
                     /locus_tag="VFP93_00950"
     CDS             1381..2289
                     /locus_tag="VFP93_00950"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9843207.1"
                     /translation="MNIFITGANGFIGQVLCKHLQKKGYTVFAGMRKAQPFAEIPIIE
                     YGDLANACNIPHDFCPDIVIHCAARAHKIKDSSKNPKQTFHAVNVLGLRHVIQAVMKK
                     WDTAAKFMEKAPPRFILLSSIKVLGEKTLNKPFTETDPYNPQDDYAWSKAEAEQVLLQ
                     AAQEYAFKATIIRPPLVYGENAQGNIKRLKTLIRKRIPLPFASIKNKRSMIQVENICA
                     FIEQCVKNHTINGIFHVADKQYSTAEFIRYLAREEQIQPILFPCSVKILKWLGKVLNK
                     ETEINRLCESLQVDCTKAQNIPRDFA"
BASE COUNT          867 a          432 c          468 g          736 t
ORIGIN      
        1 ccctcttggg aagtagagag aaaatttttc tcaaatataa tattattccc catgccaaac
       61 ctacttttat ggcgaaatca ggctttttat ttgtagggag attagagcaa ggcaaaggaa
      121 ttgtaacctt attggaaact tggcagcact taaaacaaaa ttatatactt aaaataattg
      181 gatccggccc actcgaagat gatttaaaac gtaagtttca aaaggaaaat attgtctttt
      241 taggttccct ttcaagtggt tcagttcaat tacacatggc taacgcaaaa tttttaattc
      301 agccaagttt gtattatgaa acgtttgggt taacgatcct ggaagccatg gcacagggta
      361 caccagtcat aggatttaat attggtacac ggcctgaatt tatagaacac aacaaaaatg
      421 gatttattgt tgaatccgaa atgctagaac aaagtctatt tgagtttgaa aatatcccaa
      481 attatcataa attatgttaa aatgcttgga aaacagctgg gcaatttgaa accaaaaaag
      541 ttatactgaa gcaaattgct ttataccaac aaattatggg tgatgcatgt taaaacatcc
      601 aaaaataagt gtgatcaccg tggtgaaaaa tagtgaatcg accatagaac ataccttaaa
      661 atcggtagcc aatcaaacct atgctaacaa agagcattgg attcttgatg gcgcgtccac
      721 ggataatacg ctgaatatcg tcaaacgtta tgcgcaacaa tattcgcata tccaatggtt
      781 ttcagccaaa gataaaggta tgtacgatgc tttaaataaa ggcatgcagt tagcgaatgg
      841 cgaaatccta ggatttcttc atgcggatga tttttacgct aaaaattcgg tgttggatga
      901 agttgctctg tccttcaatc ctttcacaca agcggtttat agcgatttgc tttatgtcga
      961 ccctcatgat ctaaaaaaaa taaaacgcta ctggcaatcc aaaccttatg agaagggagc
     1021 ttttgcgcgt ggttggtgcc cgccacatcc aacttttttt gtaaaaaaaa ctacgtatga
     1081 aagattggga ggcttcgata ctagcctgaa tttgggcaat gatgtggaac tcatgatgcg
     1141 ctttatggaa aaacataacg tacccaccaa atattgcccg ggggtttggg tggtgatgcg
     1201 cgcgggcggt atttccaata aaagtattaa aaatattttg caacaaaatt tatctattat
     1261 gcaatccttt aaaaaacatg atttaccctt ttcttggatg tattttatat taggaaaatg
     1321 catcgatcga ctaaagcaaa ttgtacataa acatcatgtc gattattcgt tggaaaatgc
     1381 atgaatatat ttataacagg ggcgaatggc tttataggcc aagtattgtg caaacactta
     1441 caaaaaaaag gatatactgt gtttgcaggg atgcgaaaag cgcaaccttt tgctgaaatt
     1501 cccattattg aatatggtga tcttgccaac gcttgtaaca taccgcatga tttttgtcct
     1561 gacattgtaa ttcattgtgc ggctcgagca cataaaatca aagattctag caaaaaccca
     1621 aaacaaacct ttcacgcagt gaatgtttta ggtttacgcc atgttataca agcggtgatg
     1681 aaaaagtggg atactgcagc aaaattcatg gaaaaggctc ctccccgttt tatattactg
     1741 agctctatta aagttttggg cgaaaaaacg ttgaataagc ccttcacaga aacggatccc
     1801 tataatcccc aagatgatta tgcatggtct aaggcagaag cagaacaagt gttgttacaa
     1861 gctgcccaag aatatgcttt taaagcaacg ataattcgac cacctttggt gtatggggag
     1921 aatgctcaag gaaatataaa gcgtttaaaa accttaattc gcaagagaat tcctttgcct
     1981 tttgctagta taaaaaacaa acgtagtatg attcaagtag aaaatatatg cgcttttatc
     2041 gagcaatgtg taaaaaacca tactataaat ggcatttttc atgtggcaga caaacaatac
     2101 tctacggcag aatttatacg atatttggcg cgtgaagaac aaatacaacc cattttattt
     2161 ccctgttctg taaaaatttt aaagtggtta ggtaaagttc taaataaaga aacagaaatc
     2221 aatcgtttat gtgaatcttt acaagtcgat tgcacaaaag cgcaaaatat tcctcgggat
     2281 tttgcttaat ttttaaaaat ttcataaaat actgccgact ttctttctaa gaaaaaaaag
     2341 atacaataca tagcctaatg ttaagaggaa aagcttttga ccagcgtttt tgattttagc
     2401 caacatacta acaatgcgga tcttaaagaa gagggaaaat ctcctctgat aaatcttcaa
     2461 caaggtgtaa ttgtcatcat ttcattaaaa aatgcagtaa ttc
//
LOCUS       DASTIB010000057         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1381538, whole genome shotgun sequence.
ACCESSION   DASTIB010000057 DASTIB010000000
VERSION     DASTIB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381538"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFP93_00955"
     CDS             complement(<1..200)
                     /locus_tag="VFP93_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9843208.1"
                     /translation="MNKKILIIVTSNDELGDSGEKTGFHLSELTIPFFTFLEAGYHID
                     IASPKGGEAPIDPNSYDLKIDE"
     gene            complement(227..913)
                     /locus_tag="VFP93_00960"
     CDS             complement(227..913)
                     /locus_tag="VFP93_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9843209.1"
                     /translation="MNKKTVLITGANRGIGFEFAKQYAEDGWDVFAGIRSDTKELQNL
                     IRHHNITIVKLDLNNLQKCKENLHFFLSKPLHLLINNAGVYGSNNQSILDDLTEVDFF
                     EVFQVNTIAPFLLTQFLLPTLRKGDKIIVNITSRMGSIEDNSSGGSYIYRASKAALNA
                     VMKSMQIDLAEEGFKVLLLHPGSVKTDMGGKHALIEPEESVRNMRRIIENAHQLENQF
                     YHYQGDELPW"
     gene            1101..1415
                     /locus_tag="VFP93_00965"
     CDS             1101..1415
                     /locus_tag="VFP93_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843210.1"
                     /translation="MKKVINLSLIAALTLSLGALFSASSFAAGNNDDNRDNNRNHCKS
                     NAMEMTAAEDECKDKLTCKTGEKINCKEKIGNNKDRWSCKCEKSDNNGDNMNNNTNNR
                     GM"
     gene            1465..2433
                     /gene="rtcA"
                     /locus_tag="VFP93_00970"
     CDS             1465..2433
                     /gene="rtcA"
                     /locus_tag="VFP93_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HET9843211.1"
                     /translation="MIIIDGAFEEGGGQIIRTSMALSAITQKPIEIIHVRANRERPGL
                     QPQHLTACLAVREITNGNLRFATLKSQHFTFEPGPIKGGMYEFKIGTAGSATLVAQTV
                     LPILLHAKEPSEIIIYGGTHLTHSPSFDYFQKVFEPALRLFGIHMESTLHNCGFYPKG
                     GGKIAIKIAPQPILGNSNWNAPNYLQGIIRLVGLPEHIAQRELKILQANGIQDIEIYH
                     QQYNLSPGNSILLWAPYVGGSGLGERGKPAEKVAEEALKAYKLENSEIDCHLADQLLL
                     YAALAKGQTQYTTSLHTSHLITNAHIINQFLRRNITLGPNGLVSIQ"
BASE COUNT          787 a          458 c          447 g          781 t
ORIGIN      
        1 ttctcatcga tctttaagtc atagctattg ggatcaatag gagcttcacc acctttggga
       61 cttgcaatat cgatgtgata ccctgcttct aaaaaagtaa aaaatggtat ggtgagttcc
      121 gataaatgaa aaccggtttt ctctccagaa tctccgagtt catcgttact ggttactatg
      181 attaatatct tcttgttcat aaactgttcc ttgttcgtta gattaactac caaggcaatt
      241 catcaccttg ataatggtaa aattggtttt ctaattgatg cgcattttca atgatacgtc
      301 gcatattacg tacagattct tcgggttcga tgagcgcatg tttcccaccc atatctgttt
      361 tgacagaacc cgggtgtagt aaaagcactt tgaacccctc ttctgctaaa tctatttgca
      421 tgcttttcat tacagcattt aatgctgctt tgcttgctcg atatatataa gatccgcccg
      481 aggaattatc ttcaatactt cccatgcgac ttgttatatt cactataatt ttgtctccct
      541 ttctcagggt gggtaataaa aattgtgtta ataaaaaagg cgctattgta ttaacttgaa
      601 atacttcaaa gaaatccacc tctgtaagat catccaaaat ggattgatta ttcgaaccat
      661 aaactccagc attgtttatt aacagatgta agggttttga tagaaaaaaa tgcaaatttt
      721 ctttacattt ttgtaaatta tttaaatcaa gcttcacaat agttatgtta tggtgtctga
      781 ttaaattttg cagctctttt gtgtcgctgc gaatacccgc aaaaacatcc caaccatctt
      841 ccgcgtattg ctttgcgaat tcaaaaccaa tacctctgtt ggcaccggta atcagtaccg
      901 tctttttatt cataacacat taggtaatta ataatataac tatcatagca ataaattatt
      961 tcatgcataa aaccattata tagttgcttg attaactcgc gcattttgtc ctatagattg
     1021 tcacgacctg gcgcttcttt ttgataaatc catcaattat tgcgccacaa cactatagtt
     1081 agaatgcatg gagagtactc atgaaaaagg tgatcaatct ttctttaatc gctgctttga
     1141 cgttaagctt gggtgcactt tttagtgcta gcagttttgc tgcaggcaac aatgatgaca
     1201 atagagataa caatagaaat cattgtaaat caaatgctat ggaaatgact gcagctgaag
     1261 atgaatgtaa agataaatta acatgtaaaa cgggtgaaaa aattaattgt aaagaaaaaa
     1321 ttggtaataa taaagataga tggagttgta agtgtgaaaa atccgataat aacggagata
     1381 acatgaataa caacaccaat aatcgcggta tgtaatacta ttgtctttaa aacccccttt
     1441 tgggggtttt tttatggata atgcatgata atcattgatg gtgcgtttga ggaaggcggt
     1501 ggtcaaatta tacgaacgtc aatggccttg tccgctatta cacaaaaacc gattgaaatt
     1561 attcatgtcc gtgcaaatcg tgaacgtcca ggattgcaac cgcaacattt gactgcttgt
     1621 ttagctgtgc gcgaaattac gaatggtaat ttgcgctttg ctaccttaaa aagccaacat
     1681 ttcactttcg agccaggccc tataaaaggg ggaatgtatg aatttaagat cggaaccgca
     1741 ggctctgcta ctttagttgc acaaacggta ttacctatat tattacacgc aaaagaacct
     1801 agcgaaatta ttatctacgg cggtacacat ctgacacact ctccaagttt tgattatttc
     1861 caaaaagttt ttgaacccgc attacgcttg tttggaatcc acatggaaag cacattacat
     1921 aattgtgggt tttaccccaa aggtggggga aaaattgcaa taaaaattgc acctcagccc
     1981 atactaggaa atagcaattg gaacgcgcca aattatctgc aaggtataat acgattagta
     2041 ggtttacctg agcatattgc gcagcgagaa ttaaaaatat tgcaagctaa tggaatacag
     2101 gatattgaga tttaccatca acaatataac ctttctcctg gtaacagcat cttattgtgg
     2161 gccccttatg ttggaggttc tggtttagga gaacgcggaa aaccagctga gaaagttgca
     2221 gaagaagctt taaaagccta taaattagaa aattcagaga ttgattgtca tcttgcagat
     2281 caattgcttc tttatgcggc tcttgcaaag ggtcaaacgc aatatacaac ctcccttcat
     2341 actagtcatc ttattaccaa cgcccatatc attaaccaat ttttaagaag aaatataaca
     2401 ttgggtccaa acggtttagt gtcaatccaa tgagctaact cagttaaagc tgttgtattt
     2461 ttcaagagtt tta
//
LOCUS       DASTIB010000058         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_219189, whole genome shotgun sequence.
ACCESSION   DASTIB010000058 DASTIB010000000
VERSION     DASTIB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219189"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFP93_00975"
     CDS             <1..522
                     /locus_tag="VFP93_00975"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9843212.1"
                     /translation="RKDSNIDVLSKLKPVFSTNGTITAGNSSQMSDGASALLLVSEKF
                     LTKYDLQPLARLKSYKVAGVAPEYMGIGPVKAIPKVLKQVGLKTHELDWIELNEAFAA
                     QSLAVIEELNLDIKKINPQGGAIALGHPLGATGAIRACTLIHGLRKRNLKYGMVTMCI
                     GTGMGAAGFFEAL"
     gene            690..2042
                     /locus_tag="VFP93_00980"
     CDS             690..2042
                     /locus_tag="VFP93_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HET9843213.1"
                     /translation="MTKNKTNQFKSSPLLPRIFGFTFLTVSSTVFAAGFQIQEQNVTY
                     LGTAYSGTAAWAADASTAFYNSAGLTHIPYKQVVASGVLILANSDFNVTTSNVPAIFG
                     IPVPGQQSDDPGGLIAIPSLYYSAQVGRDWFFGLSVASPFGLKTVYDENAIMRYVATR
                     SELRTIDIAPSLAWRAQPWLSLSAGFDALYAEAQLDVMTNFGAGLPDGDGFQKNRAND
                     WAFGWHAGFLIDFNENSRIGLQYRSKFRVKAEGNSENLSAGGFIPAGTFLTRRVNSSV
                     ELPETVVLSGYHAFNKCWAFLADLAWTRWSRFDALRLTYFPPSPPPLPSIDTVTHENF
                     KDVWRVAVGLSYAYNNHLEFRTGLAWDESPVRDQYRTARIPDEDRIWLGFGVGFNVNE
                     NLHVDLGYAHLFFKDASLLDRGPSSVVTGLPLLPANLVQGDYDSNANIFGIQIRYNFV
                     "
     gene            2218..2835
                     /locus_tag="VFP93_00985"
     CDS             2218..2835
                     /locus_tag="VFP93_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9843214.1"
                     /translation="MPRKTDKRQRLIEAAKVLIHQQGFNLTTLADIAQEADVPLGNVY
                     YYFKTKEAIGIAVIEKRSSEWSERLASWSEHPDPLTRLTMLVQNGLDDLEITARFGCP
                     IGGLCQELGKQGGALSDLAAKLLHDILEWSEEQFRTLGFGENSSLQALNLVSSIQGMY
                     LLTHTFKDPKIASRQSVQLQNWLEGLVQRKSEVTKTPHMQEEVAI"
     gene            complement(2986..>3453)
                     /locus_tag="VFP93_00990"
     CDS             complement(2986..>3453)
                     /locus_tag="VFP93_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843215.1"
                     /translation="KLSTMQIRYIRVLLTRSIVMQKFHRWGKLFFICSLFLSSTQVWS
                     APPTEIIFSNFTDLSLNTSIAGLPGNGIDPMISKPVSYGIVTIGCNFAQALTNCPIEF
                     RDRANGNHVATVRINAIEASLVEPPIFYGEYANRYKVTGWETVPIKEITIQKI"
BASE COUNT         1044 a          615 c          729 g         1065 t
ORIGIN      
        1 cgcaaagatt ctaatattga tgttttatca aaattaaaac cagtatttag cacaaatggt
       61 accatcaccg caggtaatag ttcacaaatg agtgacggag caagtgctct tttattggta
      121 agtgaaaaat tcttgacaaa atatgattta caacccttag ctcgcttaaa aagttacaag
      181 gtagccggtg ttgcacccga gtatatggga atcggtcccg ttaaagcaat tccaaaagtt
      241 ttaaagcaag ttggattaaa aacccatgaa ttagattgga ttgagttgaa tgaggctttt
      301 gcagcgcaat cacttgcggt aatagaagag ttaaacttag atatcaagaa aataaatccg
      361 caaggtggag caatagcatt aggccatcca ttaggagcca caggtgccat tcgtgcttgc
      421 acattaatac atggattacg taaacgcaat ttaaaatatg gcatggtaac gatgtgtatt
      481 ggtacgggta tgggagctgc aggttttttt gaggctttat aaaagacagt taacattggc
      541 ttgatttttt tagtcaaaaa gaccaaaatc gtaaaattaa ctttttagct agtttgcgat
      601 tccatatcaa gacagtgtca catactatat tttcagtata ttcacaaact aaaaacctcc
      661 gcataacaag aagagggaag gagtcctgag tgacaaaaaa caaaactaac caattcaagt
      721 catcacctct attgcccagg attttcggat ttacattttt aactgtctcg agtacagttt
      781 ttgctgcagg attccaaatc caagagcaaa atgttactta cctaggtaca gcgtatagcg
      841 gcacagcagc atgggctgct gatgcaagca ctgcatttta taattcggca ggcctcacgc
      901 atattcctta taaacaagtt gtcgcatctg gtgttttgat tttggcaaat tcagacttta
      961 acgttaccac gagtaacgta ccagcaattt ttgggattcc tgtgccggga cagcaatcag
     1021 acgatccggg tggtttaata gcaattccat ctttatatta ttcggcgcaa gtgggaagag
     1081 attggttttt tggtttaagt gttgcatctc ctttcggatt aaaaaccgtt tatgatgaaa
     1141 atgcgataat gcggtatgta gcaacgcgat cggaactgcg aacaattgat attgcaccaa
     1201 gccttgcttg gcgtgctcag ccttggttat ctttatctgc aggatttgat gcgctttatg
     1261 cagaagctca acttgatgta atgaccaact ttggtgcagg cttacctgat ggtgatggtt
     1321 ttcaaaaaaa tcgcgccaac gattgggctt tcgggtggca cgcaggattt ttaatagatt
     1381 tcaatgaaaa ctctcgcata ggtttacaat accgatctaa atttagagta aaagcagaag
     1441 gaaatagtga aaacttgtcg gctggtggtt ttattccggc aggtactttt ctaaccagaa
     1501 gagtaaattc ttcggtagaa ttaccggaaa ctgttgtcct gagtggttat catgctttca
     1561 ataaatgctg ggcattttta gcggatcttg cttggactcg ctggagtcgt tttgacgcct
     1621 tacgattaac ttattttcct ccgagtccgc caccactgcc ttctattgat acagtaacgc
     1681 atgaaaattt taaagatgtt tggcgtgtag cggttggtct tagttacgca tataataatc
     1741 atttagaatt tagaacaggc ttagcatggg atgagtcgcc tgtaagagat caataccgca
     1801 cagcacgtat acctgatgaa gatagaatat ggttaggctt tggtgtcggt tttaacgtta
     1861 acgaaaactt gcatgttgat ttgggttacg cgcacttatt ctttaaagat gcaagtttat
     1921 tagatcgtgg tcccagctct gttgttacag gattgccatt gcttcctgct aatttagtac
     1981 aaggggatta cgattcgaat gcaaatattt tcggtattca aatcagatat aatttcgtat
     2041 aattgcttat gttagaattg tactgtagta ggggttatgg ttaaaattta acatacattg
     2101 tattgcggtt tggtgtacgt aataaatttt tgtatgttgg aaatttaatc atatgttgtg
     2161 taaggtttgg ggtgaatgga tgcagtcatc ccatggttag aatggactta agacgttatg
     2221 ccacgtaaaa cagataaacg acaaagactt attgaagctg cgaaggttct aatacaccaa
     2281 caaggtttta atttaacaac cttagcagac attgctcaag aagcagatgt gcccttgggg
     2341 aatgtttact actatttcaa aacaaaagaa gcgatcggaa ttgcagttat agaaaaacgt
     2401 tcttctgagt ggtcagagcg tttagcctct tggtctgaac atcctgatcc tctgacacgt
     2461 cttacaatgt tagtgcaaaa tggtttagac gatttagaaa ttaccgcgcg ttttggatgt
     2521 cccatagggg gattatgtca agaattaggt aaacaaggcg gcgccttatc cgatttagca
     2581 gcaaaattgt tgcacgatat tttagaatgg tcggaagaac aatttagaac attaggcttt
     2641 ggtgaaaatt cctcactaca agcgctaaat ttagtttcga gtattcaagg tatgtatcta
     2701 cttacgcata ctttcaaaga tccaaaaatc gcctctcggc aaagtgtcca attacaaaat
     2761 tggttagaag gtcttgttca aagaaaatcg gaagttacaa aaacaccaca tatgcaagag
     2821 gaagttgcaa tttaattata cttttcgcat gagcttttgg gtttgttgtc ttcgcctact
     2881 tcgcaatcct catgtatgcc atatacattt cggagatgct caggggtttg gcgcttaccc
     2941 aaaaaatcct ctgctttgag tataggataa acccccgtat gggggttaaa ttttttgaat
     3001 agttatttct tttatgggca cagtttccca tcctgttact ttatatctat tcgcatattc
     3061 accgtaaaaa atgggcggct ctactaagct tgcttcaatc gcattaattc taacggtagc
     3121 aacatgattg ccatttgctc gatctctgaa ttcaattggg caattagtta gtgcttgagc
     3181 aaaattgcaa ccaatcgtta caattccata agagacgggt ttgctaatca tgggatcgat
     3241 tccattaccg ggtaatcctg caatagaggt gttaagggac aaatccgtaa aattagaaaa
     3301 tattatttct gtgggtgggg cagaccaaac ttgagtgcta cttaaaaaca aactacatat
     3361 aaaaaaaagt ttaccccaac gatgaaattt ttgcattaca atactccttg ttaacaacac
     3421 ccgaatatat cggatctgca tcgtagaaag ctt
//
LOCUS       DASTIB010000059         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1381358, whole genome shotgun sequence.
ACCESSION   DASTIB010000059 DASTIB010000000
VERSION     DASTIB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381358"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..215)
                     /locus_tag="VFP93_00995"
     CDS             complement(78..215)
                     /locus_tag="VFP93_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843216.1"
                     /translation="MTTPIGNLGQKALNYMPSLEHLQFMKKSLDEDNVKPDGWFFSDL
                     L"
     gene            214..>2017
                     /locus_tag="VFP93_01000"
     CDS             214..>2017
                     /locus_tag="VFP93_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843217.1"
                     /translation="MTFFEILEKINKIQKLQASENSNSRIEAEQDIKELENAVRGELF
                     SLIRPLIQNTNFCELQIPAFAQSYGKYFNPGVYHELLCYTHLAHCFEPNGIPQEQALK
                     LAILFADTDSALRYITNSGCTLHDACLFQLPPVDECHFPFWQKLAKKSMLDQRFRKLL
                     PYANEIENLMMSAAKPKLDYQLINAKKNAINHVHHEWKQFKRKHGILNDQEKLKFQKL
                     TQDLLLLKDELVELCKGISLHNTDLTILDAFYQYYQKTANPAYHYFLQNGLTKKDYDK
                     FIALDRKDAGKNIPDLMVEGNEIGHPGFYLRKLPVHDDNYAALGACLGKLTNCCQSLS
                     GEAGESCVIHGLTDPNGGFYILCKGDSKDQKSTDEILAQTWVWRSKLNVLVFDSVEIS
                     KKADIKMTVDFFRILAKDLVSKNFSPKVNCGNSSGISTRVGLNLNDVPEQFINYKGYT
                     DSEHQNVVYKNNNPFHWYGNDHWYWYGKDQNYTAFINHKIHESIDENVPFAKVAFLCQ
                     GINWAISKQQFSILEGFKEIAKTLDRFDEIKNLIMSMELFVNKPTKNFIKDYEEQLKT
                     YILNKQYCMDTFNNEGKTLLMVACLQGEENFAKFL"
BASE COUNT          706 a          321 c          357 g          633 t
ORIGIN      
        1 aggttaattt cgttaccaac tttatatagt cgatgccaca cgttttagac actttttatg
       61 aagtataaga cctgtagcta taacaagtca gagaaaaacc acccatccgg ttttacatta
      121 tcttcgtcga gagatttttt cataaattgc agatgttcaa gcgatggcat ataatttagt
      181 gctttttgac ccaagttgcc aatcggagtt gtcatgacat tttttgaaat tcttgaaaaa
      241 attaacaaaa tccaaaaatt acaagcaagc gaaaattcga attctcgcat agaagcggaa
      301 caagatataa aagaattaga aaatgcagtt cgtggtgaac ttttttcatt aattcgtcct
      361 ttaattcaga ataccaattt ttgcgaacta caaattccag cattcgcgca atcctatgga
      421 aagtatttta atcctggcgt ttaccacgaa ttattatgtt atacgcattt ggcacattgt
      481 tttgaaccca atggcatacc tcaggagcag gccttaaaat tggctatatt atttgctgac
      541 accgattcgg cgctaagata tattacaaat agtggttgca ccttacatga tgcctgctta
      601 tttcaattgc cacctgtaga tgagtgtcat tttccatttt ggcaaaaact agccaagaaa
      661 tctatgctgg atcaacgctt tcgtaaattg ctgccgtatg cgaatgaaat agaaaatctt
      721 atgatgtccg ctgctaaacc aaaacttgat tatcagttaa taaatgccaa aaaaaatgcg
      781 attaatcatg tacatcatga atggaaacaa tttaaacgta aacatggtat tcttaatgat
      841 caagaaaaat tgaaatttca aaaattaact caggatttat tactattaaa agatgaactg
      901 gtagagcttt gtaaaggtat ctctttacat aacacagacc taacaatttt agatgctttt
      961 tatcaatatt atcaaaaaac tgcaaatcct gcataccatt attttctgca aaatgggcta
     1021 actaaaaaag attatgataa atttattgca ttagatagaa aagatgcagg taaaaatata
     1081 cccgatttaa tggtagaagg aaacgaaata gggcatccgg gcttttattt aaggaaactg
     1141 ccggttcacg atgataatta tgcggccctt ggtgcttgct taggaaaatt aaccaactgt
     1201 tgtcaatcct tgtcggggga agctggtgaa tcttgcgtta ttcatggttt aaccgatcca
     1261 aatggtggat tttatatttt atgtaaaggt gatagtaaag atcaaaaaag cacagatgaa
     1321 atattagcac aaacatgggt ttggcgaagc aaactgaatg ttctcgtatt tgattcggtt
     1381 gaaatatcca agaaggcaga tataaagatg accgtagatt tttttagaat tcttgctaaa
     1441 gatctggtct ctaagaactt ttcccctaaa gtcaattgtg ggaattcgtc aggtatatcg
     1501 actcgcgttg gattaaattt aaatgatgtt cctgagcaat ttattaatta taaaggctac
     1561 acagattcag agcatcaaaa cgttgtgtac aaaaataata acccattcca ttggtacggc
     1621 aatgatcatt ggtattggta tgggaaagac caaaattata cagcatttat aaatcataaa
     1681 atccatgaat ctatagatga aaatgtaccg tttgctaaag ttgcttttct ctgtcagggg
     1741 attaattggg ccatatcaaa acagcaattt tctattttag aaggttttaa agaaattgct
     1801 aaaactctgg atcgatttga cgaaatcaaa aacttgataa tgagcatgga gctatttgtt
     1861 aataaaccga ccaaaaactt tattaaggat tatgaagaac aattaaaaac ctatattctt
     1921 aacaaacaat attgtatgga cacattcaat aatgagggaa agacattgct tatggtagct
     1981 tgtttacagg gcgaagaaaa ttttgcaaaa tttcttt
//
LOCUS       DASTIB010000060         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_561767, whole genome shotgun sequence.
ACCESSION   DASTIB010000060 DASTIB010000000
VERSION     DASTIB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561767"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFP93_01005"
     CDS             <1..922
                     /locus_tag="VFP93_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843218.1"
                     /translation="QSEELLQQIRDFSEEIASKNDISRKQHAQALINYEAQVRLKEYE
                     LEEQKMYMAAPGSPEQIDAGLAIAKDVSANQAKQIVDSAMQRGLPVGQVSGIGSQIEL
                     INYLKPYLNNGDVNSIRGLDISSILQEDTVDPATVNRLIDTLTNPFPQTLSVSRDENG
                     EIAPTPAEMKTISDRVLEQAMMSVAVNALGELGAKRFVNPDTNKSLMQIMHEESIRRF
                     QNVNWYKNIGISSQEALLRELAHMEAFRLRMQYEHYRLQEQTVSLLSALVASQAKMTS
                     MIQMLNETMMQASQNAQDAMPANVEDIDLE"
     gene            964..>2267
                     /locus_tag="VFP93_01010"
     CDS             964..>2267
                     /locus_tag="VFP93_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HET9843219.1"
                     /translation="MKRNKVRLYKWTGFIYVPFLFGLVYVALHYPQYLVYLGILGFVP
                     TVLLILLALQPKSQQRFDVPNTKFGLWFFKVLFFEIALLALVFAHAISFFGAGPQFAT
                     QNISLESLYAAIAQNHWLWWSFFPWGCLTIWVVCLHYYYYEKKTGPYPHQTVQYLLKG
                     FLEPQTKGFIETTIYSVNLICISTTLVAAILLSSYAIQVFLGLIPHTLITPITYALLG
                     VLVLILFWWLKPNRLRHLPQQNFTLSRLSIILALSFILTLSLASMLSQWLIQHPKFSI
                     DIAQLQCDCQKYFDSTSTENRIISALWGWCLLWIPLCGSYLVKISQGRSVREMILGIN
                     VLPAFLFLGCAFYPDLLFRNLNNIKDYVVIINASSKALLLLSLGLIAFGFCYLFTQKQ
                     TNNKMFYAGALEDYTAAGRTRLSQGCKTIGLSKFAFSFFSAVL"
BASE COUNT          679 a          406 c          447 g          735 t
ORIGIN      
        1 gcagtcagaa gagcttttac agcaaattcg cgattttagt gaagagattg ctagtaaaaa
       61 tgatatttcg cgtaagcaac atgcccaagc cttaatcaat tatgaagcgc aagtccgatt
      121 aaaagaatat gaacttgaag aacaaaaaat gtatatggca gcacctggaa gtcctgaaca
      181 aatcgatgca gggcttgcaa tcgcaaaaga tgtctctgct aaccaagcaa aacaaatcgt
      241 agattctgcc atgcaacgtg gcttgccagt tggacaagtc agtggaattg gttcgcaaat
      301 agagttgatt aattatttaa agccctattt aaataatgga gatgttaatt ctataagagg
      361 attagatata tccagcatct tgcaagaaga tactgtagat ccagcaaccg taaatcgact
      421 tatcgatacc ttaacgaatc cgtttccaca aaccttaagt gtttctcgag atgaaaatgg
      481 cgaaatagcg cccacaccag cggaaatgaa aactatctcc gatcgggtgt tagaacaagc
      541 gatgatgtcg gtggcagtca atgccttggg cgaactggga gcaaaacgtt ttgttaatcc
      601 agataccaat aaaagcctta tgcaaattat gcatgaagaa agtattcgaa gatttcaaaa
      661 tgtaaattgg tataaaaaca tagggatttc gtctcaagaa gcattattac gagaacttgc
      721 tcatatggag gcatttcgat tacgtatgca atatgagcat tatcgattgc aggagcaaac
      781 cgtgagtttg ttatccgctc ttgttgcctc ccaagctaaa atgacatcta tgatccaaat
      841 gttgaatgaa actatgatgc aagcatcaca gaacgcacaa gatgctatgc cggcgaatgt
      901 tgaagatata gacttagaat aggatctaaa aggcagctta acgctgcctt tcaagcaagc
      961 aatatgaagc gtaacaaagt tcgcctatat aaatggacag gttttattta tgttcctttt
     1021 ttatttggtt tagtttatgt agctctacat tatccccaat atcttgtata tttaggaatt
     1081 ttaggttttg tacccacagt cttgctcatt ttgttggctt tgcagccgaa aagccaacaa
     1141 cgttttgatg tacccaatac aaaatttggc ctatggtttt ttaaagtact cttctttgaa
     1201 attgccctac tggctttagt gtttgcacat gctataagtt tttttggggc aggtccacaa
     1261 tttgccaccc aaaatataag tctagaatcg ctgtatgcgg ctattgcgca aaaccattgg
     1321 ctttggtgga gcttctttcc ttggggctgt ttaactattt gggtcgtatg tttgcattat
     1381 tattattatg aaaaaaagac aggcccttat ccgcaccaaa cagtgcaata tttactaaaa
     1441 ggttttttag aaccccaaac caaaggattt atcgaaacta cgatttattc cgtcaattta
     1501 atttgtatta gcacaacgtt ggtggcagca attttgcttt catcatatgc aattcaagtt
     1561 tttcttggac tgattccgca tacgcttatt acacctatca cgtatgcgtt attgggtgtc
     1621 ttggtgttaa tacttttttg gtggttaaag ccgaatcgtt tacgacactt accacagcaa
     1681 aattttacgt tgtctcgtct aagtattatc ttagcgttaa gttttattct taccttgagt
     1741 ctagcaagta tgttgagcca atggctcatt caacatccaa agttttcgat agatattgcg
     1801 cagctacaat gtgattgtca gaaatatttt gatagtacga gcaccgaaaa tcgcataatc
     1861 agtgcattat ggggatggtg tttgttatgg attcctttgt gcggctctta tttggtaaag
     1921 atttcccaag gtcgcagtgt gcgtgagatg atcttaggta ttaatgtatt acccgctttc
     1981 ttgtttttgg gatgtgcttt ttatccagat ttactcttta gaaatttaaa taatataaaa
     2041 gattatgtag taattattaa cgcctcatcc aaagctttat tgctattatc cttaggttta
     2101 attgcttttg gattttgtta tttgtttacc caaaagcaaa ccaataataa aatgttttat
     2161 gcaggcgctt tagaagatta tactgctgcg ggacgcacac gcttgagtca aggttgtaaa
     2221 actataggct taagcaaatt tgctttttca tttttttccg ccgtgct
//
LOCUS       DASTIB010000061         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1456073, whole genome shotgun sequence.
ACCESSION   DASTIB010000061 DASTIB010000000
VERSION     DASTIB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456073"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VFP93_01015"
     CDS             <1..1312
                     /locus_tag="VFP93_01015"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9843220.1"
                     /translation="KAIAFAGIPGEVAAIAHISKNYARFSLVEDLKPAIRLARKGFAA
                     DQAFVLALEKFSSILQKSPQSGRIFFDKGQIPSPGYTIVQEDLADILEAIGYEGREGF
                     YEGKTAEKLVEGVNQLGGNWLLEDLKKYQVKIRKPLKANFRDWTITTVPPPSAGGLTI
                     LTSLRVLDRFEEDPLSTSDRIHLIVESFRRAHCDRVRFVSDPDFYKTPDALTTDTHIE
                     QLRDSIEFFKASNAKTFPCEPLSNHGNTTHFSIIDKEGNRVAATLSINDFFGTGLMVP
                     GTGILLNNELADFSFADKKSPNRLESSKRPVSSMSPLFLESSDTLAILGTPGGLRIPS
                     MLLLAILAAEEGQAPKQWLKNPRFHFNFTQNVIEYESGAFSQPVKNALQLRGHQLKEV
                     EPYGNMQLVYLDKKNEILSAFSDPRWFGKAMVQTASSSDTLRVN"
     gene            complement(1287..2603)
                     /locus_tag="VFP93_01020"
     CDS             complement(1287..2603)
                     /locus_tag="VFP93_01020"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET9843221.1"
                     /translation="MRKNYGYGIGICFLILLFGLGYSLKSTPFIDLTTHKNHTLTPVT
                     QTLLKSFTAPLQFTLTTSDPDLHQNFIFFLEKFAAINKNIKSEIRWVSNVDSTKPYAH
                     EVLEVTYNQKTEVISLLQKPLNEITMQNVLIRLQQGQEHWIVFLSGHGEPALLGDSPK
                     DFGNLAKALKSQGLKVANLNLATTGFIPDNTSLLIIVDPQTAWLPKEEEQIAHYIKQG
                     GALLWLKDFQGKTPQVLSKLLGVEVLPGIIVDQHGYALGTPHPAITLITEFSNPILPQ
                     PMQICAFPWAQALKLSESSWNAIPILKTHPQTWTARGKLEGAIEFNAQRGEIQGPLLL
                     GVTLEKTHSQRNSRIVVIGNHRFATNAVIHNYGNMTLSLNLFNWLIGENGLTKIEYSS
                     QKDALFQIPAWYAFVLKFGFGLFLPLLILGLSFIFHKRTNLRATYR"
     gene            complement(2603..>3350)
                     /locus_tag="VFP93_01025"
     CDS             complement(2603..>3350)
                     /locus_tag="VFP93_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843222.1"
                     /translation="KMFQLTHYEILKILRQRGNWFALAFWIFVSSLLFFYLLKDYTFQ
                     KQLAVAQMGVTQGVVKPFWGWMLFVLYIGAPMLTILNLGLEHSQKSYHFWILHRFPVS
                     QYIFPKLLGLLTVQTLGLLLILAIPLSLFSVTTIDFGLLCSGFVTVWCMMFFMVSLSL
                     ALYVATLNAFFSLMLTYSCVLIFSIMPGFSTFPKLTQWLNYVSPLQHAHGLMNGRLYV
                     TDVFYFVFIITILIAFCLSKMQKRFRKLDY"
BASE COUNT         1076 a          623 c          661 g          990 t
ORIGIN      
        1 taaagcaata gcatttgcag gcattccagg cgaagttgca gctattgcgc atatttcaaa
       61 aaattacgcc cgcttttctt tggtcgaaga cttaaaacct gctattcgtt tagcacgtaa
      121 agggtttgcc gccgatcaag catttgtgtt agccttagaa aaattttcct ctattctgca
      181 aaaatctccc cagagtggtc gcattttttt tgataaaggt caaattcctt ccccaggcta
      241 tacaatcgtc caagaagatt tggcagatat attagaagcg ataggctatg aaggaagaga
      301 aggcttttat gaaggaaaaa cagcggaaaa attggtcgaa ggcgtaaatc aattaggagg
      361 aaattggtta cttgaggatc tcaaaaagta ccaggtaaaa atacgcaaac ctctcaaggc
      421 aaattttcgg gattggacta tcactacggt gcctcctcct tcggcaggtg ggcttactat
      481 tctaacaagt ttacgcgttc ttgatcgttt tgaagaagat ccactgtcta cttccgatag
      541 aattcattta attgtagaaa gctttcgccg tgcgcactgt gacagagtgc gttttgtcag
      601 tgacccagat ttttacaaga cgccggatgc gttaactacc gatacgcata ttgagcaact
      661 tcgagactct atagagttct ttaaagcatc caacgcaaaa acctttccct gtgaaccact
      721 ttccaatcat ggcaacacta cgcatttttc tattatcgat aaagaaggta atcgcgtggc
      781 ggcaacctta agtatcaatg atttttttgg aacgggtttg atggttcctg gtacgggcat
      841 attattaaat aatgaattag ccgatttttc gtttgcagat aaaaaatcac cgaatcggct
      901 agaatctagc aaacgtccgg tttcgagcat gagtccgtta tttttagaat cttctgacac
      961 gcttgcaatt ttaggtactc caggtgggct tcgtattcct tctatgttat tacttgcgat
     1021 tttagcagca gaggaaggac aagccccgaa gcaatggttg aagaacccta ggtttcattt
     1081 taattttacc caaaatgtta ttgaatatga atcgggagct ttttcacaac ccgttaagaa
     1141 tgctttgcag ttacgaggac atcagttaaa agaagtagag ccttacggca atatgcaatt
     1201 agtctatttg gataaaaaaa atgaaatact aagtgccttt tctgatccac gttggtttgg
     1261 aaaagccatg gtacaaacgg cctcttctag cgatacgttg cgcgtaaatt agttcttttg
     1321 tgaaagataa aactcaagcc taggatgagc aaaggtaaaa aaagtccaaa accaaatttt
     1381 aatacaaagg cataccaagc aggaatttga aacagcgcgt ctttttgcga ggaatattct
     1441 atcttggtta agccattttc tccgattaac cagttaaaaa gatttaaact caacgtcatg
     1501 ttgccgtaat tatggatcac cgcattggtt gcgaagcgat gattaccgat taccacgatg
     1561 cgtgagtttc tttgcgaatg ggttttttcc aacgtgactc caagcaatag tggaccttga
     1621 atttcaccgc gttgtgcgtt aaattcaata gcgccttcca atttaccacg tgctgtccat
     1681 gtttgaggat gggtttttaa gatagggata gcattccaag aagattcgga taactttagg
     1741 gcctgggccc aaggaaaagc gcagatttgc atcggttgag gcaaaattgg gttagaaaat
     1801 tcggtgatta acgtaatggc agggtgtgga gtgcctaaag catagccgtg ttgatcgact
     1861 attattcccg gcaatacttc aacacctaat agtttcgaaa gaacttgagg cgttttacct
     1921 tggaaatctt taagccacag tagagcgcca ccttgtttta tataatgcgc aatttgctct
     1981 tcttccttag ggagccaagc tgtttgtgga tccacaataa ttaacaaact tgtattgtcg
     2041 ggtataaaac cggttgtagc taaatttaaa tttgctactt tcaatccttg acttttaagt
     2101 gcttttgcaa gattaccaaa gtcttttggt gaatctccta aaagggcggg ttccccatgt
     2161 ccacttaaaa acacaatcca atgttcttgt ccctgctgta agcgtattaa tacattttgc
     2221 atggttattt catttaaagg tttttgtaac aaggaaataa cttcggtttt ttgattgtat
     2281 gtgacctcca atacttcatg cgcataaggc ttggtagagt ccacattcga tacccagcga
     2341 atttccgatt taatattttt atttattgct gcaaattttt ccagaaaaaa gataaaattt
     2401 tgatgcagat ctggatcgga tgtcgttaac gtaaattgta ggggcgcggt aaaacttttc
     2461 agcaaagtct gagtgacagg cgttaaggta tgatttttat gtgtggttaa atctataaat
     2521 ggtgtagatt ttaagctata ccctaggcca aacaataaaa ttaaaaaaca aattccaatt
     2581 ccataaccgt aattttttcg catcagtaat ccaatttacg aaaccgcttt tgcattttgc
     2641 ttaagcaaaa tgcaattaaa attgttatta taaaaacaaa ataaaaaaca tccgttacat
     2701 acaatctgcc attcataagg ccatgtgcat gttgtaaagg acttacataa tttaaccatt
     2761 gggtgagttt tggaaaagtg ctaaatccag gcattatact aaagattaaa acacaactat
     2821 aggtgagcat taaagaaaaa aatgcattca aggtggcgac atataacgca agactcaaac
     2881 ttaccataaa aaacatcatg caccaaacgg ttacaaatcc cgagcataat aaaccaaaat
     2941 ctatggtggt aacggaaaat aaactaagcg gaatagcaag tataagtaaa agacctaaag
     3001 tttgcacggt taaaaggcct agtaattttg gaaaaatata ctgagaaacg ggaaaacgat
     3061 gcaaaatcca aaaatgataa gatttttgac tatgttctaa acccaaattt agaatggtta
     3121 acataggtgc tccaatatac aacacgaaca acatccaacc ccaaaagggt tttacgactc
     3181 cttgggttac acccatttgt gccacggcta attgtttctg aaacgtataa tctttgagta
     3241 aataaaaaaa taagagagaa ctcacaaaaa tccaaaaagc tagcgcaaac caattgccac
     3301 gttggcgcag gattttcaaa atttcatagt gcgtaagttg gaacatttta
//
LOCUS       DASTIB010000062         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1053656, whole genome shotgun sequence.
ACCESSION   DASTIB010000062 DASTIB010000000
VERSION     DASTIB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053656"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFP93_01030"
     CDS             <1..342
                     /locus_tag="VFP93_01030"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039393.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9843223.1"
                     /translation="RERLEEIVTRTRQGGTEIVSLLKTGSAYYAPAAAAIEMAESYLK
                     DQKKILPCAAKLTQGQYGITEPLFVGVPTKIGSHGVEEIIEVSLTEKERKNLQISIQA
                     VTELNQAVAHL"
     gene            complement(391..2757)
                     /locus_tag="VFP93_01035"
     CDS             complement(391..2757)
                     /locus_tag="VFP93_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007143967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET9843224.1"
                     /translation="MKIFLRLFLRVLFSTALTGCCILLIVILYTNAKLPSVSNLEDIQ
                     LQVPLRIYTRDGKLIGEFGETRRNPIRIHEVPEKLKLAILATEDRRFYEHHGVDLRGL
                     ARAGVHLITRGNKDQGASTITMQVARNFYLTRKKTFARKLNEILLAIKIEKELSKDQI
                     LELYLNKIYFGKRAYGVQAAAYVYYGMPVSKLNLAQMAMLAGLPQAPSAINPLNNPEA
                     ALKRRTHVLNRMKHYGFITEHEYKKAMKQPIHAQYHGPKVEVHAPYVAEMARQWLVDK
                     YGSKIYTDGFEVYTTVDTRLQKKAQDAMQSGLLAYDKRHGYRGPAGHVKLPKQVKNAN
                     WQALLAPYLEFSHLLPAVVTHIEDHKLVAYLSNGQAIDVYFKHMTWARPQHKFYLGST
                     PKTVSDVAKKGDIIYVESINKEWSLSQVPVVSGALVSLNPKNGAIISLVGGFDFRISS
                     FNRANQAYRQCGSNFKPFIYSTALDRGFTAASIINDAPLVFHDSALDEVWRPQNDTRK
                     FYGPTRLRVGLTKSRNLVTIRLLQAIGLPAAREALRKFGFERNRLPDGLSLALGTINA
                     TPLEIATGYAGFANGGYKVSPFIVEKVIDPSDKVIYKADTSEVCTWCDEITLDDAPTH
                     PSIMTPQAAYIMDSILKDAIQTGTGQLAKKLNRHDIAGKTGTTNQQMDAWYSGYNPDI
                     VTTVWVGFDEPKSLQEYAAQAALPIWVDFMGYALKNKPEKTLPEPPGIVTVRIDPNTG
                     LLASPGATNAIFEVFKEENVPTRSASTMAQERESVTLTPNNNSDETLF"
BASE COUNT          898 a          599 c          607 g          874 t
ORIGIN      
        1 cgcgagcgtt tagaagaaat agttacccgc acccgccaag gtggcacaga aattgtaagt
       61 ttgttgaaaa cgggctcggc atactatgca cccgcggcgg ccgcaatcga aatggcagaa
      121 tcgtacctaa aagatcagaa aaaaatctta ccctgtgctg ctaaattaac ccaaggtcaa
      181 tatggaataa ccgagccact atttgtgggc gttcctacaa aaattggatc tcatggtgta
      241 gaagaaatta tcgaagtttc actgaccgaa aaagaaagaa aaaatctgca gatatccatt
      301 caagcggtta ctgagctgaa tcaggcagtg gcgcatttgt aatggagtga acgcaaaatc
      361 ccagccaaac tgggattttt gtgtttttat ttaaaataag gtttcatcgg aattattgtt
      421 aggcgttaaa gtaacggact ctcgttcttg tgccatagtc gaggcacttc gcgtaggtac
      481 attttcttct ttaaaaactt caaaaatagc gttcgtagca cctggagatg ctaataatcc
      541 tgtattggga tcgattctta cggtaacaat accaggaggt tcgggtaaag ttttctcggg
      601 tttgtttttt aaggcgtatc ccataaagtc tacccaaata ggaagagcag cttgggctgc
      661 gtattcttgc aaagatttgg gttcgtcaaa tcctacccat acggtggtaa caatatctgg
      721 attatatcca gaataccatg catccatttg ttgattcgtc gttcctgttt tcccggcgat
      781 atcatgacgg tttaattttt tggctaattg ccctgtgcct gtttggattg catccttaag
      841 gatagaatcc ataatataag cagcttgcgg cgtcataatg gagggatgtg ttggagcatc
      901 gtccaacgtg atttcgtcac accacgtaca tacttcagag gtgtctgctt tgtaaatgac
      961 tttatcggaa ggatcaataa ctttttctac gataaaagga gaaactttat agccaccatt
     1021 cgcaaaacct gcataacccg ttgcaatttc taagggtgtg gcattaatgg tgccaagggc
     1081 taacgacaag ccatccggca aacgatttct ttcaaatcca aatttgcgca atgcttcacg
     1141 agcagctggc aagccgattg cttgtagcaa acgtatcgtc actaaatttc tcgattttgt
     1201 aaggcctacg cgcaaacgtg ttggaccata gaattttcgc gtatcatttt gaggacgcca
     1261 aacttcatct aaagcagaat cgtgaaaaac taaaggagca tcattgatga tacttgcagc
     1321 cgtaaagccg cgatccaaag cggtactata aatgaatggc ttaaagttcg atccacattg
     1381 gcgataagct tgattggctc gattaaaact cgatattctg aaatcaaatc cacccaccaa
     1441 actgataata gcaccatttt tggggttcaa agaaaccaac gctccactca ccaccggaac
     1501 ttggcttaaa ctccattctt tgtttatgga ttctacgtaa ataatatcgc ctttttttgc
     1561 aacatccgat acggtttttg gggttgatcc taaataaaat ttatgttgcg gacgtgccca
     1621 agtcatatgt ttaaaataaa catcgatggc ttgcccgttc gataaatagg caacaagttt
     1681 atgatcttct atatgggtaa ctacggctgg taataaatgg gaaaattcta aataaggtgc
     1741 taaaagcgcc tgccaatttg cattttttac ctgtttaggt aattttacgt gacctgcagg
     1801 ccctcgatag ccatgacgtt tatcataagc caataaacca ctctgcatag cgtcttgcgc
     1861 ttttttctgt aaccttgtat ccacggtggt ataaacttca aacccatcgg tgtaaatctt
     1921 tgagccgtat ttgtctacca accattgacg cgccatctca gctacgtagg gcgcatgcac
     1981 ttccactttc ggaccatggt attgcgcatg gatgggttgc ttcattgctt ttttatattc
     2041 gtgctcggta ataaagccat aatgcttcat acgattgaga acatgggtac gacgctttaa
     2101 ggcagcttca ggattgttta aaggattaat agcggaaggt gcctgaggca atcccgccaa
     2161 catggccatt tgtgctaaat ttagttttga aactggcatt ccataataaa cataggccgc
     2221 ggcttgcact ccgtaagctc gcttaccaaa ataaattttg tttaaataga gttctaaaat
     2281 ctgatcttta ctgagttctt tttcgatttt tattgccagt aaaatctcat ttaatttgcg
     2341 agcaaaagtc ttcttgcgtg taagataaaa gttcctggct acctgcatag taatggttga
     2401 agcaccttga tctttattgc cccgcgtaat gagatgaacg ccagcacgcg ccaaaccacg
     2461 taaatctaca ccgtgatgtt cgtagaagcg tcgatcttcg gttgctaaaa tagcgagttt
     2521 taatttttca ggaacttcat gaatgcgaat ggggttacga cgcgtttcac caaattcccc
     2581 aataagctta ccgtctcgcg tataaattcg taatgggact tgaagctgaa tgtcttccaa
     2641 atttgaaacg gaaggcaatt ttgcatttgt ataaagtata acaatgagca aaatgcagca
     2701 gccggtcaaa gcagtgctga acagaacgcg caaaaaaaga cgaagaaaaa ttttcataat
     2761 ttaataatat actaaataag caactaactc gttatcaagt atgttatcat acattggcat
     2821 aatattataa gattcattgg aaagttgcct aaagataact aattggaaac ttaagatcta
     2881 tatttttaat aaggattcgc acaaaatagc actaatattg cgagcaaaga aagagatcta
     2941 attaccttat gtaaaatatg ttgtttcgcc aaaaaata
//
LOCUS       DASTIB010000063         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1440859, whole genome shotgun sequence.
ACCESSION   DASTIB010000063 DASTIB010000000
VERSION     DASTIB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440859"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFP93_01040"
     CDS             <1..1050
                     /locus_tag="VFP93_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843225.1"
                     /translation="ESVGRLEEMQFLLDSIGYFEANCLDINSLYLQKKPSLDFEKIIK
                     LIQEHKFFINTQNQDGTTVLILAANQGLVPVVRALLNLGADLHMANMHGSALTWAIVG
                     AQNKRNFTMHKVIELLIQHGLDPNQGINQSVPALIQATELNLLPIVTKLLDLNIDINV
                     KDHTGKTALMVASSLGHDNIVLELMENGVDLHATDASCSNALMYAVINQHEDIVNELV
                     ENGINLNAQNNNGYTALMLAVDLGFVDIAKLLLEHNADPNVKDPSGKTALVIAAQKHC
                     SSVITALLLAGADDIEALHYLSSKGMIQEKQFIEKHIRKANVKQANVIFSQRQPSDAQ
                     LNETQSATGSKKLQP"
     gene            complement(1059..1856)
                     /locus_tag="VFP93_01045"
     CDS             complement(1059..1856)
                     /locus_tag="VFP93_01045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9843226.1"
                     /translation="MAKDDFIAFKEIQKQSWSLFAPLEVVTTMCAATLVNFSKLSHEN
                     VVLDVGCGTGVVAITAARLGAKISAMDLSPALIDHAKKNIAIAKVEVDLQEGDVENLP
                     YPDASFDVVLSQFGHMFAPRPSIATHEMLRVLKPGGTIAFSTWPPDLFTGRLFSLVGK
                     FLPLPEGVSPPPLWGDPHFVRAQLGTAVDDLTFDQGVMRFPAMSLGHYRQSVETTIGP
                     VFKLVEESKKDSARLQAFRDALEELASQYFTDNHIHQHFLMSRARKK"
     gene            complement(2026..3531)
                     /locus_tag="VFP93_01050"
     CDS             complement(2026..3531)
                     /locus_tag="VFP93_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843227.1"
                     /translation="MKRSHSQEQINPTKRNKNNNASAEIKAHYPHLPIDGDNLDKCLA
                     NKDSQAFKNLADFIANEDVETILDTIDFNATRTHYKLTSDSQEYYFSFLALICLNSIR
                     SLENLALFEKILERIPLHHLDFNVSLDNEINALKNLICSSPIAELPVLPILKFFEKNP
                     YPYLNFNLKFNNGQTPFSILTTYASQFPQLLIKVFENVPFENIDWTVKNPYNRLPNEL
                     PILWEIFWAATYSAGPEAIADTFIKNYANNLHNLDFTVRIPSGPTLEEWLLSTQLAPH
                     MHKINLIIQINKTRELWKSLCAEVATNTESLEKMDLFQDHIKKLNDLVTLAAQQNPDA
                     YYVLATFYHESGLVDQLKEVAFNLDKEGLYRDKIYCMLADELAYGDPTDLEAAEPSKL
                     FKQAKHQKQERKKVLEEALDYAIEGKNSQLCRTIGINYCHSLEPNAGFQFPQEPLLDN
                     LTGGKQTALYAMQTLRKKIKLEQKVAQKDAFIQEQDARIKELEAKLATFTR"
BASE COUNT         1133 a          671 c          686 g         1156 t
ORIGIN      
        1 gaaagcgtgg gacgcttgga agaaatgcaa tttttgctag attctattgg ctattttgaa
       61 gcaaattgct tagatattaa ttccttatat ttgcaaaaaa aaccttctct agattttgaa
      121 aaaataatta agcttattca agaacataaa ttttttatta atacccaaaa ccaagatggc
      181 actacggttt taattttggc agcaaaccaa ggccttgtgc ccgttgtgcg agcattactg
      241 aatcttggag cggatctcca catggctaat atgcatggaa gcgccttaac atgggccata
      301 gtaggtgccc aaaataaacg taattttact atgcacaaag tcattgaatt acttattcaa
      361 catggactgg atcccaatca aggtattaat caaagcgtac ctgctctaat tcaggcaaca
      421 gagctgaacc ttttgcctat tgtgaccaaa ttacttgatc tgaacattga tattaacgtt
      481 aaagatcaca cgggtaaaac tgctttaatg gtagcatcaa gtttaggcca tgataatatc
      541 gttcttgaac tcatggaaaa cggtgttgat cttcatgcga cggatgcatc gtgtagcaat
      601 gcgctcatgt atgccgtaat aaatcaacac gaagatatag taaatgaact tgttgaaaat
      661 gggatcaatc ttaacgccca aaataataat ggttacactg cacttatgct ggctgtggac
      721 ttaggatttg tagatattgc gaagttgcta ttagagcata atgcagaccc taatgtaaaa
      781 gacccaagtg gtaaaacagc gcttgtcata gccgcgcaaa agcattgttc ttctgtgatt
      841 actgctttat tgcttgccgg agcggatgat attgaagcgt tacattacct gtcatcaaaa
      901 ggtatgatac aggaaaaaca gtttattgag aaacatatac gtaaagcaaa tgtaaaacaa
      961 gctaacgtta ttttttctca aagacaacct tctgatgcac aattaaatga aacgcagagt
     1021 gcgaccggat caaaaaaatt acaaccatga taatgggttc attttttgcg agctcgactc
     1081 atcaaaaaat gctggtgaat gtggttatct gtgaaatatt gtgacgctaa ttcttccaat
     1141 gcatcgcgaa atgcttgcaa gcgtgcagaa tcttttttag attcttccac aagtttaaat
     1201 accggaccaa tcgtggtttc gaccgattgc ctatagtgtc ccagactcat cgcgggaaac
     1261 cgcataacgc cctgatcaaa tgttagatca tccactgcag ttcctaattg cgcgcgtaca
     1321 aaatgcggat cgccccacag cggtggtggt gaaacacctt cgggtaaggg caagaacttc
     1381 cctactaaac taaacaaacg tccggtaaat aaatcgggtg gccaagtaga aaatgcgatt
     1441 gtgccacctg gttttaaaac acgtaacatt tcgtgggttg ctatagaggg acgtggtgca
     1501 aacatatgac caaattgact aagaaccaca tcaaagcttg catctggata gggaagattt
     1561 tctacatctc cttcttgcaa atcaacttcc accttggcaa tagcgatatt tttttttgca
     1621 tggtcaatca atgctgggct taaatccata gcacttattt ttgcgcctaa tctcgctgcg
     1681 gtgattgcga caacccctgt accacaacct acatcgagaa caacattttc atgactcagt
     1741 ttagaaaaat ttactagggt tgcagcacac atggttgtga caacttctaa aggtgcaaac
     1801 aaactccaac tttgcttttg tatttcttta aaagcaatga aatcatcctt tgccatgatt
     1861 tgctccttga cctacttagt attctagaaa actaacctag ttatcttgcg atgtgcaatg
     1921 gttactataa actgaattaa agaggtatgg taggaataaa gtttaaggat ttaagaaggt
     1981 catagaaagc tcaagttgat acttgagctt tacgttgttt caaaattatc gggtgaaggt
     2041 cgccaatttt gcttccagtt ctttaattct tgcatcctgc tcctgaatga atgcatcttt
     2101 ctgcgccacc ttctgttcca gcttgatttt tttccgtaaa gtttgcattg catacaacgc
     2161 agtttgtttg ccacccgtca aattatctag caagggttct tggggaaatt gaaacccagc
     2221 gtttggctca agggaatgac aataattaat gccaatggtt ctgcataatt gcgaattttt
     2281 gccttcaatt gcataatcta aagcttcttc tagcactttt ttacgctcct gtttttggtg
     2341 ctttgcttgc ttaaagagtt tggaaggttc cgctgcttct aaatccgttg gatcaccata
     2401 agctagttca tctgccagca tacaataaat tttatcacga taaagaccct ctttatctaa
     2461 gttaaaagct acttctttta attggtcaac taaaccgctt tcgtgataaa aagtggctag
     2521 tacataataa gcgtctggat tttgttgagc tgcaagcgta accaaatcgt ttagtttttt
     2581 aatatgatct tgaaataagt ccattttctc caaagattcg gtatttgttg ctacttcagc
     2641 acataaactc ttccataatt ctcgcgtttt atttatttgt ataattaaat ttattttatg
     2701 catatgcgga gctaattgtg tacttaacag ccattcttct aaagttggac ccgagggaat
     2761 tcgcacagta aaatctaaat tgtgaagatt gttagcataa ttttttatga aagtgtcggc
     2821 tatagcctca ggacccgccg aatacgtagc agcccaaaag atttcccaaa gtatagggag
     2881 ctcattaggc aaccgattat aagggttttt tactgtccaa tcaatatttt cgaaaggaac
     2941 attctcgaat actttgatta ataattgcgg aaactgcgag gcatacgttg ttaaaattga
     3001 aaaaggcgtt tgaccattgt taaactttaa attaaaattt aaataagggt atggattctt
     3061 ttcaaaaaat ttcagaatgg gcagcaccgg aagctcagct ataggtgatg aacaaattaa
     3121 attttttaac gcatttatct cattatccaa ggatacgtta aaatctagat ggtgtagagg
     3181 aatgcgttct aaaatctttt caaaaagggc caaattttct aatgatctta tcgaatttaa
     3241 gcaaattaat gctaagaaag aaaaataata ttcttgtgaa tcggacgtca atttataatg
     3301 agtacgtgtg gcattaaaat ctatagtgtc aagaatagtt tctacatctt cgtttgcgat
     3361 aaaatcggcc aaattcttga atgcttgcga atctttatta gctaagcatt tatccaaatt
     3421 atcaccatca atcggtagat gtgggtaatg cgcttttatc tctgcgcttg cattattatt
     3481 tttgtttctt ttcgtggggt taatttgctc ttgcgaatga cttcgtttca taataaatat
     3541 ttctataaaa aggaaacttg atatcatttt tccttgaaaa gtacaatcat aagtaggcaa
     3601 aagataggag gacttgtata agtagtaaaa ttattacgca ttttta
//
LOCUS       DASTIB010000064         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_918749, whole genome shotgun sequence.
ACCESSION   DASTIB010000064 DASTIB010000000
VERSION     DASTIB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918749"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VFP93_01055"
     CDS             <1..1339
                     /locus_tag="VFP93_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HET9843228.1"
                     /translation="GFITMQLTRVFGSLADSYGHIVRTNLAEVDLKDVVLNRRILVVL
                     LPALEKSPEELSNLGKIIIASLKAMMAAGLGDEVEGDYRDVILRKPTTSPTPYVCIMD
                     EYGYYAVEGFAVVPAQARSLGFSAIFAGQDLPAFQKASKEEAASIGANTNIKICMKME
                     DPTDTWEFFNKTAGETYATSVSGFQTNAGSLTNNYLDSRNASIEKRQRIDLLDLKDQR
                     EGDAHIFWRSKIIRARMFYANPKPVKRLQLNQYITVEKPSDTTLMNIHKRITRFNQIF
                     QAPNFVMQSPAPNEDITTVALAMNEYHNLKPFERGIAAVISLLEKEVEKQRKVREEIH
                     EEASQEFNNEVHAFTPVFLPEVAKLIVPPDELERFTSPLLFKGRTREAIVHIERLAGK
                     SEAEANKIADELIKDVIKATYYPPDVHLPRNPQLLLDALEEFYEILTGQPYTE"
     gene            1440..>2255
                     /locus_tag="VFP93_01060"
     CDS             1440..>2255
                     /locus_tag="VFP93_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843229.1"
                     /translation="MNIGPMILQGVLEILQNALKSQAATLNDAQISNLAQRWHRYEIA
                     LGTLTAQANIPMESTIKNIAVAYEDAISAIGLTEHQKKQVSLAKTLFMLIKKVHINLQ
                     EEIHKLYFKQKKLEKVLLLLTQESKLRALELDLHLAILLHQESLRQQKLLELQNLEPH
                     RLLLLELKTELDSILTTYQTSLSQLHSQRDILRERQSSIDMQLQSVSEQKTHLQNAIV
                     IATEDLQKIVSAMEKTPKILEELVQFTQTSNAQIQTLESDLILWNKAEAEIEGL"
BASE COUNT          761 a          449 c          436 g          609 t
ORIGIN      
        1 cggttttatt accatgcaat taacacgggt atttgggtcg cttgccgaca gttatggtca
       61 tattgttcga accaatttag cggaagtaga tctcaaagac gttgtattaa atcgacgcat
      121 actcgtggtg ttattgcctg ctttagaaaa atctcctgaa gaactctcca acttaggaaa
      181 aattattatt gcttctttga aagcaatgat ggcagcgggt ttaggggacg aagtagaagg
      241 ggattatcgg gatgttattc tacgtaagcc taccacctcg cccacccctt atgtgtgtat
      301 tatggacgag tatggctatt atgcagtaga agggtttgcg gtcgtgccgg cacaagcgcg
      361 gtcgttagga ttctcagcca tttttgcagg acaagattta ccagcgttcc aaaaggcctc
      421 taaagaagaa gcggcgtcta ttggcgcgaa caccaatatt aaaatttgta tgaagatgga
      481 agatcctacc gatacgtggg agtttttcaa taaaaccgca ggtgaaactt atgcaaccag
      541 tgttagtggg ttccaaacga atgccggaag tttaaccaat aattatttag attcgcgcaa
      601 tgcctcgatt gaaaaaagac aaagaattga cttattagac ttaaaagatc agcgcgaagg
      661 ggatgcacat attttttggc gctctaagat tattcgcgcc cgaatgtttt acgcaaaccc
      721 caaacctgta aaacgactac agcttaatca atacatcaca gtagaaaaac cctccgatac
      781 caccttaatg aatattcata aacgcattac ccgatttaat caaatctttc aagcgccgaa
      841 tttcgtgatg caatcccctg caccaaatga agatattact accgttgctc ttgcaatgaa
      901 tgaataccat aacctaaaac cgttcgagcg agggattgcc gctgttattt ctttgctgga
      961 aaaagaggta gaaaagcaac gcaaagtccg agaagagatt catgaagaag caagtcaaga
     1021 atttaataac gaagttcatg cctttacccc cgtctttttg ccggaagttg ctaagctaat
     1081 tgttccaccc gatgaactag aacgatttac ttcacctttg ctttttaaag ggagaacgcg
     1141 agaagccatt gttcacattg aaagattggc tggaaagtcg gaagcagaag caaataaaat
     1201 tgcagatgaa ttaattaaag atgttattaa agcaacctat tacccaccgg atgtacattt
     1261 gccccgtaat ccgcaacttc tacttgatgc tctcgaagaa ttttacgaaa ttttaaccgg
     1321 ccagccgtat acagaataac tggtttttta tccttttggt gtgtgtaaaa gcctatgtta
     1381 caatgaaagc taactcatat taatgggtaa ttaaccctca caataacgag gtagttttct
     1441 tgaacatcgg ccccatgatt ctgcaaggtg ttttagagat tttacaaaat gcattaaaaa
     1501 gccaggctgc gacgttaaat gatgcacaaa tttccaatct tgcacagcgc tggcaccgtt
     1561 atgaaattgc tctagggacg ttaacagcgc aagcaaatat tcccatggag tctacgataa
     1621 aaaatattgc cgttgcatac gaagatgcga taagcgccat aggtttaacg gaacatcaaa
     1681 aaaaacaagt cagcctggct aagacgcttt tcatgctcat aaaaaaagtg cacataaacc
     1741 tacaagaaga aattcataaa ctttatttta aacaaaaaaa attagaaaaa gtcctgttgc
     1801 ttttaaccca ggaatcaaaa ttacgtgcac tagaactcga tttacactta gccattcttt
     1861 tgcatcaaga aagtttgcgc caacaaaaac ttttagaatt acaaaattta gaaccccatc
     1921 gtcttttatt attggaatta aaaacagaat tagattcgat ccttacaacc tatcaaacct
     1981 cactatcaca gttacattcc caacgagata ttttaagaga acgacaaagt agcatcgata
     2041 tgcaattaca atctgtatct gaacaaaaaa cacatttaca aaatgcgata gtaatagcga
     2101 ccgaggattt acaaaagata gtttctgcaa tggaaaagac accgaagatt ttggaagagc
     2161 ttgtgcagtt tacccaaacc tcgaatgcgc aaatccaaac attagaatcc gatttgatct
     2221 tatggaataa agcagaagca gaaatagagg gctta
//
LOCUS       DASTIB010000065         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1426559, whole genome shotgun sequence.
ACCESSION   DASTIB010000065 DASTIB010000000
VERSION     DASTIB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426559"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1676)
                     /locus_tag="VFP93_01065"
     CDS             complement(48..1676)
                     /locus_tag="VFP93_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9843230.1"
                     /translation="MQFFLGVAIFALVARLSFLQIFNKDFLNAQGNARILRTVTIPSY
                     RGLVTDRRGSPIAVSTPVDSIWVNPKHFHATQSQLTQLSSLLALPPHDIVTKIKQYSH
                     RSFLYLKRALTPEHAAKIEKLNIPGIYTQREFRRYYPAGPVTAQLLGFTNIDDEGQAG
                     IELAFDQWLKPIPGKKRVLEDRMGHWIQDVDHSLVPRQGGNLALSIDLRIQTLAYNAL
                     NEALKRLNAKSATLVMLDIVSGEVLAMVSVPSFNPNVYHERTSKGVRNKALTDQIEPG
                     STAKALSMVSLLEHSPFSIDDEVDTNPGFYRLGGHLIRDVRNFGKLTLSQVLVKSSNI
                     GLSKFIIQRPSEELLNTFKSFGLGTDITNFFPGENSGRLPSPTKSPIVTATWSFGYGF
                     TATPLQLVHAYATLASRGKKQPISLLKLEDTIPKGDQIISSETAEHVIRMLTEVLNTS
                     GTGRRASIKGYKTAGKTGTTRLVGSSGYDADRHNALFIGFTPVDEPRLATLVLIEEPD
                     ESNDHYYGGFAAAPVYAQVVRSALHMLNVPPDNA"
     gene            1782..2096
                     /locus_tag="VFP93_01070"
     CDS             1782..2096
                     /locus_tag="VFP93_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HET9843231.1"
                     /translation="MAIIYEVNMQVDSSILIEYKLWLEDHIADMLTLPWFLDAKMYEE
                     LRTDAQVNLCIHYHLASQQELESYFKQDAQKMRTRGERYWPHVKFQRRILMERNKHAQ
                     SN"
     gene            2080..2466
                     /locus_tag="VFP93_01075"
     CDS             2080..2466
                     /locus_tag="VFP93_01075"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9843232.1"
                     /translation="MHNQTEPQGDLVSRTIAMPRDTNRYGDIFGGWLLSQMDLGGAVL
                     AHKCSHDRMTTVAVDGMMFIKPVYVGDLVSCYASVQRKGNTSVTISVEAWVERHKDNS
                     FHQVTRGLFTYVAINDEGRPTPIQWR"
     gene            complement(2467..4359)
                     /locus_tag="VFP93_01080"
     CDS             complement(2467..4359)
                     /locus_tag="VFP93_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9843233.1"
                     /translation="MATKNQFQLLLERRFLPYFITQFLGAFNDNVYRISLMILIAVTY
                     ASAEKSQADYLVNLCAALFIMPFFLFSATAGQLADKYTKAYIIRWIKFSEVVMMLLAG
                     FAFYLNSIYFLIVILFFMGTHSAFFGPIKYSILPQHLNKRELVGGNGLVEMGTFIAIL
                     LGTIVGGLLIGLEHYPKLAVSITTISVALLGFITSFFIPKIKSPSSEEPQLKIRLEPI
                     SETWKTLKIAASKRVIFLSILGTSWFWFYGTSFLTQLPNYNARFIHGNESVITLMLAA
                     FSIGIGVGSMLCERLSGHKVEIGLVPFGAIGLTLFAADLSFAHGPFPEVEITVREFIQ
                     SPHNWRLLADLTLIGLFGGFYIVPLYVILQDRSKPNQRSRIIAANNIINSLLMALASV
                     LAVVLLRVGLSIPEFFFVIALMNAAVTLYIFKVVPEFLMRFIIWILIHSLYRVEVEGS
                     ENLPEDGPAVLCCNHVSYLDPLIVMACVRKPIRFVMDYHIFKIPILSFVFKTAKCIPI
                     TPGKHNRQIKENAFKEVSKALKARQLVGIFPEGGLTPNGSVQPFKPGISKIALDNNVK
                     IIPIALQGMWGSWYSRRHGRAMLHLPRKFIFAKVKVKVGAPIYAKDFDLQSLQQKIEE
                     LRGNMP"
     gene            4688..>5355
                     /locus_tag="VFP93_01085"
     CDS             4688..>5355
                     /locus_tag="VFP93_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HET9843234.1"
                     /translation="MIIPVILAGGSGSRLWPLSREAFPKQFIPLFDSESLLQKTLTRF
                     IALPDVTSPIIICSEAHRFLVQDILVKQHSSVLATILEPTPKSTAPAIALAAFQALET
                     YEDPLLLVLPADHLIEDKAGFQHTVLQAIQAARNGYLVTFGVTPTAAETGYGYIQCGE
                     KISGAVSKVAGFIEKPPKAQAQQLMQSNQYLWNSGMFLFSARAFLQELNNCQPALSKK
                     VELS"
BASE COUNT         1673 a         1067 c         1071 g         1544 t
ORIGIN      
        1 gaagtgggcg aagacaacaa ccctgaaaaa gcatctgtga aaagtaatca agcgttgtcg
       61 ggtggaacat tgagcatatg aagcgcgctg cgcacgactt gcgcatatac tggcgctgct
      121 gcaaatccgc cataataatg atcgttagat tcatcaggct cttcaattaa aacaagtgtg
      181 gcaagtcttg gttcatcgac gggggtaaag ccaataaaaa gcgcattatg tctatccgca
      241 tcgtaacccg aactacccac aagacgggta gttcccgttt taccggcagt cttatagcct
      301 ttaatacttg cacgccgtcc tgttccactg gtatttagca cttctgtaag cattctaatg
      361 acatgttctg cggtttcgct ggatatgatt tgatcgcctt ttggaatcgt atcttccaat
      421 tttaagaggc taatgggttg ttttttccct cttgatgcca atgtggcata agcatgtact
      481 aactgaagtg gggttgcggt gaaaccatac ccaaacgacc atgtagcagt cacaatgggg
      541 cttttggtag gagagggaag gcgtcctgaa ttttcgccag gaaaaaagtt ggtaatatcc
      601 gtacctaagc caaaactttt aaaggtattt aatagttctt ccgaaggcct ttgaatgata
      661 aatttactta aaccaatatt gctggattta accagtactt gcgacagcgt aagcttaccg
      721 aaattgcgta catcgcgtat aaggtggcca cccaatcggt aaaaaccagg attggtgtct
      781 acttcatcat caatagaaaa aggactgtgt tctaaaaggc taaccataga tagcgcttta
      841 gcggtagaac cgggttctat ttgatccgta agcgccttat tacgcacgcc tttactggta
      901 cgctcgtgat acacgttagg attaaaggat ggcacactca ccatagctaa aacttcaccg
      961 ctcacaatgt cgagcataac taaggttgct gatttagcgt ttaggcgttt tagcgcttca
     1021 tttaaagcat tgtaagcgag tgtttgaatt cttaagtcga tactaagtgc aagatttcct
     1081 ccttgacggg gtactaaaga atgatccaca tcttgtatcc agtgccccat gcgatcttca
     1141 agtactcttt ttttaccggg gataggtttt agccattgat caaatgcaag ctctattcca
     1201 gcctgacctt catcgtcaat attggtgaat cctaatagtt gcgccgtaac gggacctgcg
     1261 ggatagtatc ggcgaaattc gcgctgggta tagatacccg gaatatttaa tttttcaatt
     1321 tttgccgcat gttctggagt caatgctctt tttaaatata aaaaacttct atgagaatat
     1381 tgtttaattt ttgtgacaat atcatgagga ggcagtgcta gaagagaact taattgcgtt
     1441 aattggcttt gggtagcatg aaaatgttta ggattaaccc aaatggaatc tacaggcgtg
     1501 ctcacggcga tgggactgcc gcgcctatca gtaacaagac cgcgatacga cggtatagta
     1561 accgtgcgca aaatcctagc atttccttgt gcgtttaaaa aatctttgtt aaaaatttgt
     1621 aaaaaactta gtcttgccac tagggcaaaa atggccactc ctaaaaaaaa ttgcacaaca
     1681 tgcaaccgtt taacacggaa ttgccaagat tttggtttac caggtttgtt atattttgta
     1741 aatgtcaatg taagttaact tgaaaaataa ggggattacc tatggctatt atatatgaag
     1801 ttaatatgca agttgattct tctatcctaa ttgaatacaa attatggtta gaagaccata
     1861 ttgctgacat gttgacttta ccatggtttc tcgatgctaa aatgtatgaa gagctgagaa
     1921 cggatgcaca ggtaaatcta tgcatccatt atcatcttgc atcacagcaa gaacttgaat
     1981 cttactttaa acaagatgca caaaaaatgc gtacacgtgg agaaagatat tggccacacg
     2041 taaagtttca gcgtcgtatt ttaatggagc gtaataagca tgcacaatca aactgaacca
     2101 caaggcgact tagtttcaag aaccatcgca atgcctcgtg acacgaatcg ttatggcgat
     2161 atttttggtg gctggttgct ctcacaaatg gatttaggcg gggcagtgct tgcacacaaa
     2221 tgctcacacg atcgcatgac cacggtagcc gtggatggca tgatgtttat caagccggtt
     2281 tatgtgggag acttagttag ttgctatgcg agtgttcaga gaaaaggaaa cacttcggtt
     2341 actattagtg ttgaagcttg ggttgaacgt cataaagata attcttttca tcaagttacc
     2401 cgagggttat ttacctatgt tgctatcaac gatgaaggca ggccaacacc cattcagtgg
     2461 cgatagttaa ggcatattcc cgcgtaactc ttctattttt tgttgcaacg attgcaaatc
     2521 aaaatcttta gcataaatgg gtgcgccaac ttttactttc actttggcaa aaataaattt
     2581 acggggtaaa tgtagcatag ctctaccatg tcgtcggctg taccacgagc cccacatacc
     2641 ttgcaacgct atgggaataa tcttaacgtt gttgtccaaa gcaatcttac tgatgccagg
     2701 tttaaacggt tgcacactgc catttggcgt gagtccacct tcgggaaaaa tacccactaa
     2761 ttggcgcgct tttaatgctt tagagacttc tttaaatgca ttttctttaa tttgacggtt
     2821 atgttttcct ggggtaatcg gaatacactt tgcggtttta aaaacaaaac ttaagatggg
     2881 tattttgaat atatgataat ccattacaaa acgaatgggc ttgcgcacgc aagccataac
     2941 aatcagggga tctaaataac ttacgtgatt gcagcataac accgcaggcc catcttctgg
     3001 caagttttcg ctaccttcta cttctacccg atatagcgaa tgtataagaa tccaaataat
     3061 aaaccgcatg aggaattcag gtaccacttt aaaaatataa agtgtcactg cggcattcat
     3121 tagcgctatg acaaaaaaga attcgggaat gctcaatcct acccgcagaa gaaccacggc
     3181 taatacggaa gcgagggcca ttaatagtga gttaatgata ttgttggctg caataattct
     3241 cgagcgttga ttgggtttag agcgatcttg taaaattaca tagagcggaa caatgtaaaa
     3301 acccccaaac aaaccgataa gcgttaaatc cgctaataat cgccaattat gcggtgattg
     3361 tataaattcg cgcaccgtaa tttcgacttc tggaaatggc ccgtgcgcaa aacttagatc
     3421 agccgcgaat aaagtaaggc caatagcacc aaacggtact aagccaattt caaccttatg
     3481 accggataat cgttcacaca gcatcgatcc caccccgatg ccaattgaga aagcagcaag
     3541 cattaaagta ataacacttt cattgccatg aataaagcga gcattataat tcggtaattg
     3601 ggttaaaaag cttgtaccat aaaaccaaaa ccaggaagtg cctaagatcg ataaaaaaat
     3661 cacccgctta cttgcagcta tttttaacgt tttccatgtt tcagaaatag gctctaaacg
     3721 tatttttagt tgaggttctt cactggaagg cgatttaatt tttgggataa aaaaactcgt
     3781 aataaaacct aaaagagcca cacttatggt tgtaatactt actgcaagct ttgggtaatg
     3841 ctctaaacct attaaaagac cgccaacgat tgtgcccaat aaaatggcga taaaagtacc
     3901 catttccact aaaccgttac cgccaacgag ttcacgttta tttaaatgct ggggcaaaat
     3961 actatattta atggggccaa aaaaagcaga atgcgttccc atgaaaaaca aaattacaat
     4021 gagaaagtaa atactattta aataaaacgc aaagccagcc aacaacatca tgaccacttc
     4081 agaaaattta atccaccgga tgatataggc tttggtatat ttatctgcga gttgccctgc
     4141 cgtcgctgag aacaaaaaaa acggcataat aaataatgcg gcacacaaat ttactaaata
     4201 gtctgcttga ctcttctctg ctgaggcata agtcacagcg atgagaatca ttaaagatat
     4261 tcgataaaca ttatcattaa atgcaccaag aaattgtgtg ataaaataag gcagaaaccg
     4321 ccgttcaagc agtagttgaa attgattctt cgttgccatt gattaccaaa aagattttaa
     4381 gtgcagcgca ataattttgc aaaattttgc aaaattcgat cgcacccaaa tgttggaaag
     4441 tactaaatgg ttaacttcca tattcttcct taaggtaacc tagaacactt atatacttaa
     4501 aattttttgc tagacaactt tactattaat atatatttag taagttctta taattaggat
     4561 gaattttacc atcagcttaa acgcggtgca aaaatcttta aaaagtttat tgaactttgt
     4621 cggaattctc gttaatttgt tttagcttta agtatcttta gcctgcaaaa cagtaaggag
     4681 cattcagatg attatcccgg tcattttggc gggtggatct ggttcgaggc tttggcccct
     4741 ttcaagagaa gcttttccca aacaatttat cccattgttt gattcagaaa gtttattaca
     4801 aaaaacattg acacgattca tagcgttgcc tgatgttaca tcgccgataa ttatttgcag
     4861 tgaagcacac cgttttttag ttcaagatat tctagttaaa caacattctt ccgtacttgc
     4921 aacgatatta gagcctacgc caaaatccac agcacccgca attgctttag ctgcttttca
     4981 agcattagaa acgtacgaag atcctctatt attagtttta ccagccgatc atttaattga
     5041 agacaaagcg ggttttcaac atacggttct tcaagcaatt caagcggcac gcaatggata
     5101 tttagtaact tttggtgtaa cacccaccgc cgctgaaacg ggctatggtt acattcaatg
     5161 tggggaaaaa atttcaggtg cggtgtcgaa agtagccgga ttcattgaaa agccacctaa
     5221 agctcaagcg caacagttga tgcaatcgaa tcaatatctg tggaattctg gcatgttttt
     5281 attttcggca cgtgcattct tgcaagaact taacaattgc caacccgcat taagtaaaaa
     5341 agtggaactc tcatg
//
LOCUS       DASTIB010000066         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_337324, whole genome shotgun sequence.
ACCESSION   DASTIB010000066 DASTIB010000000
VERSION     DASTIB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337324"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..922)
                     /locus_tag="VFP93_01090"
     CDS             complement(122..922)
                     /locus_tag="VFP93_01090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9843235.1"
                     /translation="MPRLSVIIITKNEAHNIGACLDSVSFADERIVIDSGSSDATVAI
                     CQKYTDKILVTNWPGDGPQKNRALAMATGDWILCLDADERVTHALKKEILSTIQHTNK
                     HGFTIPYISTYCGKPLYCGDWRGEKHLRLFRRSYGIFSQDVVHCHVQLKGTLGALQSP
                     ILHHPFRNLETLLAKMNDYSTGSAQRKLEQGKHASIWTALTHGIWAFVRGYFLKLGFL
                     DGREGFMLAVSNAEGTYYRYLKLMLLAEKKNDATSTRLNVSPRFRSKT"
     gene            1030..2355
                     /gene="waaA"
                     /locus_tag="VFP93_01095"
     CDS             1030..2355
                     /gene="waaA"
                     /locus_tag="VFP93_01095"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HET9843236.1"
                     /translation="MALLLRWVYTICFVLLLPFIYLRLYRKGRRLPSYTKRWGERLGF
                     VNIPILDQSIWFHAVSVGEALAAVPLIRGFMQQNPHTNIVVTTMTPTGSDRICSAFTQ
                     DLNKNMYHAYIPYDIPWCLQRFIKKTNPQLFIAMETELWPNLFVTLQKKAVPILIANG
                     RLSPKSCRSYKKFKWAIRPMIQAIHYIATQSTIDTKRFIEIGMHPHQVENLGNVKFDL
                     QIKESDINQGSQLKQQFLGKFVWIAASTHAGEETLILQIFKQLKHNFPDLVLLLVPRH
                     PDRFEEVHALCMKTGFVVCRRSKKEYSSHLDIYLGDTMGEMLAFYAASDMAFVGGSMV
                     PVGGHNLLEPALLQLPIVTGPNLFNFAHIADLLLKAKGLKVVHDSQTLQEALLEFLRN
                     SEMRQQAGKNAYSVIAHNQGAVHKIVEKIHKIWSTSKRTFTSIPLMTKQ"
     gene            complement(2338..>3448)
                     /locus_tag="VFP93_01100"
     CDS             complement(2338..>3448)
                     /locus_tag="VFP93_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HET9843237.1"
                     /translation="SGQVSTTGINTGNQFQGDYNTKNYGLNLTQPIYRPEHWSTLEQS
                     RHTERQGLALYLAASQDLILRVSEQYFAILGAQDDLHFARAQRKAFAQQLEQTKQRFD
                     VGLIAITDVHEAQARHDSAVAAEIAAENNLSDQFELLREIIGLPLVDIAELKVTDDVL
                     LILPEPNNLEMWVETAQSHNLDIEAAREGALRAKGEVANRVTGHFPKVDVNASLARNK
                     SAPPFDDLFTQKTINLTLAIPIFEGGGVTYRVREAESRFDEAIQRLDIQRRAVYSLTR
                     QKFRGVLTQISQVRSLAQAVVSNESALRATKAAYEVGTRTVVDVLDAESNLLGAQRDY
                     AKARYEYVLEGLRLKRAAGTLKAQDLYEVNLLLGH"
BASE COUNT         1006 a          723 c          680 g         1039 t
ORIGIN      
        1 ggcatcatca aaagtcaaac aaacatttag ttttttagtg ctgagcttct tccctggaat
       61 aactatttca aaattttgca ctaaaaattc taagtggttt ataaaattat ctgtaggtat
      121 attacgtttt ggatctaaaa cgcggtgata cattaagacg agtagaggtt gcgtcatttt
      181 ttttctcggc taaaagcatt aattttaagt atcgataata cgtgccttct gcattagaga
      241 ccgctaacat aaaaccttca cgcccatcta aaaaacctaa ttttaaaaaa taaccacgta
      301 cgaatgccca gattccatgc gttagcgctg tccaaataga agcgtgtttg ccttgctcga
      361 gttttctttg cgcacttcct gtggaataat cgttcatttt tgctagtagc gtttctaaat
      421 ttcggaaagg atggtgaaga ataggggatt gtaaagcacc caacgtaccc tttaattgaa
      481 catggcaatg aacaacgtct tggctaaata ttccatagct acgacgaaag agtcgcaaat
      541 gtttttcacc gcgccaatct ccacaataaa gtggcttccc acaataggtt gaaatataag
      601 gaattgtgaa accatgcttg ttggtgtgct gaatagtaga gagtatttct ttttttaacg
      661 catgggttac gcgctcatcc gcatctaaac aaagaatcca gtcccccgtt gccatcgcca
      721 aagcgcgatt tttttggggg ccgtcaccgg gccagtttgt aactaaaatc ttgtcggtat
      781 atttttggca aatcgcaacg gttgcatcac tgctaccaga atctatcact atccgctcat
      841 ctgcaaagga gacagaatct aagcaggctc ctatgttatg tgcttcattt ttggtaatta
      901 tgataacgct taatcgtggc atggtgttct cagtccaaga aaactagata attataccta
      961 gggagttcct aagttttaac aaaaactttg gtacaatacg ggaattcaac accttggaca
     1021 aataactaaa tggctttatt gctgcgatgg gtgtatacaa tctgttttgt attgttgctt
     1081 ccttttattt atttgcgtct ttatagaaaa ggacgacgcc tgccatccta cacaaagcgt
     1141 tggggcgaac gtttggggtt tgttaacatt ccaatattag accaaagtat ttggtttcat
     1201 gcagtatcgg ttggcgaagc tttagccgcc gtacctttaa tccgcgggtt tatgcaacag
     1261 aatccccata cgaatatagt tgtaaccacg atgacgccta caggatccga tcgtatttgt
     1321 tctgctttta ctcaggatct taataaaaat atgtatcatg cttacattcc ctacgacata
     1381 ccctggtgct tgcaacgttt catcaaaaaa accaatccac aattgtttat cgcgatggag
     1441 acagaacttt ggccaaattt atttgtaact ttacaaaaaa aagccgtgcc aatcctaatc
     1501 gccaatggca gattatcccc taaatcttgc agatcgtata agaaattcaa atgggcaata
     1561 cgtcctatga ttcaggcaat tcattacatc gcaacgcaaa gtacgatcga taccaaacgc
     1621 tttatcgaaa taggcatgca tccgcatcag gtggaaaatt tagggaatgt aaaatttgat
     1681 ctccaaataa aggaaagcga tataaaccaa ggctcacaat taaagcaaca atttttgggt
     1741 aagttcgtat ggattgccgc aagtacccat gcaggagaag aaacacttat cctccaaatt
     1801 tttaaacaac ttaaacataa tttcccagat cttgttttat tattagttcc aagacacccg
     1861 gatcgctttg aagaagtgca tgctttatgc atgaaaaccg gttttgtggt ttgtcgcaga
     1921 agtaaaaaag aatattcttc ccatttagat atttatttag gggatacaat gggagaaatg
     1981 ctggcattct atgctgctag cgatatggct tttgtgggtg gcagtatggt tcccgtgggt
     2041 ggtcacaact tacttgaacc ggcgctactc caactaccta ttgttacggg accaaatcta
     2101 tttaactttg cccacatagc cgatttgttg ctcaaagcca aaggactcaa agtggtacat
     2161 gacagtcaga cattgcaaga agcattatta gagtttttac gcaatagcga gatgcgacaa
     2221 caagctggca agaatgctta cagtgttatc gcacataatc aaggcgcggt tcataaaatt
     2281 gtggaaaaaa ttcacaaaat ttggtcaact tctaaacgta cttttactag cattcctcta
     2341 atgaccaagc agtaaattaa cctcatataa gtcttgagct tttaaggttc cagctgcgcg
     2401 tttgaggcgt aaaccttcca gtacatattc atagcgcgct ttggcataat cgcgctgtgc
     2461 acctaaaaga ttcgattccg catccaatac gtcaaccacg gtacgggtac caacttcata
     2521 cgctgctttg gtagctctta aagcgctttc attggaaact acagcttgtg ccaaacttcg
     2581 cacttggctt atttgcgtca aaacgccacg aaatttttgt cgcgttaaac tataaactgc
     2641 acgtcgttgg atatccaaac gctggattgc ctcatcaaaa cgagactcag cttcgcggac
     2701 tcgataagta acaccgccac cttcaaaaat aggtatcgct aaagttaaat tgatagtttt
     2761 ttgcgtaaat aaatcgtcga aaggaggcgc gcttttattg cgagccaaag aagcgttgac
     2821 atccactttg gggaaatgtc ccgtaacacg attggctacc tcgcctttcg cgcgcaaagc
     2881 accttctctt gctgcttcaa tatctaaatt gtgggactgc gcggtttcaa cccacatttc
     2941 taaattattt ggctccggta gtattaataa aacatcgtcc gtaactttta gttccgctat
     3001 atctactaaa ggtaaaccga taatttcgcg tagaagctca aattgatcgg ataaattgtt
     3061 ctcagctgcg atttctgctg ccactgcgct gtcgtgtctt gcttgcgctt catgtacatc
     3121 ggtaatcgca attaaaccaa catcgaagcg ttgtttggtt tgttctaatt gttgggcaaa
     3181 ggctttcctt tgagcacgcg caaagtgcaa gtcgtcttgc gcacctaata tggcaaaata
     3241 ttgttctgaa acacgtaaaa taagatcttg gcttgcggcc aaataaagag ccaaaccttg
     3301 cctttctgta tgtcttgatt gttccagggt agaccaatgc tcgggtcggt aaattggttg
     3361 agttaaattt aaaccataat tttttgtatt atagtctcct tgaaattgat tgcccgtatt
     3421 aatgcccgtg gttgatactt gtccactt
//
LOCUS       DASTIB010000067         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1321621, whole genome shotgun sequence.
ACCESSION   DASTIB010000067 DASTIB010000000
VERSION     DASTIB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321621"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFP93_01105"
     CDS             complement(<1..234)
                     /locus_tag="VFP93_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843238.1"
                     /translation="MVTEVPPAEGKKTSHPTSAKRPVKTGHPRESSGLEMVLLRNNFY
                     RDNFRRLMVACLLMITAIIVLAGIIIFLIYTRPS"
     gene            complement(265..540)
                     /gene="icmT"
                     /locus_tag="VFP93_01110"
     CDS             complement(265..540)
                     /gene="icmT"
                     /locus_tag="VFP93_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HET9843239.1"
                     /translation="MRNPATAHWRDSARRPKLFFMDAYSAFPLLLFLLHIRWWTFFLC
                     IAAIAFFVILEKFGFTVPVFQRWIRAFLAGNTRIARPWWVLHKTWRG"
     gene            complement(553..1701)
                     /gene="dotB"
                     /locus_tag="VFP93_01115"
     CDS             complement(553..1701)
                     /gene="dotB"
                     /locus_tag="VFP93_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system ATPase DotB"
                     /protein_id="HET9843240.1"
                     /translation="MPELSKELLLPEEPIRFTPRDMDRLLLHCTKLGASDVTLQSKSE
                     ILAEIHGKLYPVTSRKISAAEVADMLNAIYGPNATTQISSGQDVDTHYEIKPDRETRF
                     RFRINGTGCMVEGSDAIQITARTIPSEPPDLKSMHLDQAILDSMAPVQGTVVVTGATG
                     SGKSTLLASIIKEITSQLDANRKVLTYEAPIEFVYDSVKKPSSIVAQSEIPRHLPSFA
                     AGVRNALRRKPGLILVGEARDVETIAAVIDAALTGHPVYTTLHSNGVADCVRRMVSTF
                     PAGERHGRALDILETLRLVIWQKLVPTKEGKRVALREYLVFDEEVRDILVMTDVEKLA
                     AKTRLLLKERGQPMIVDAQKKFEEGIIEERVYKILAASAKKLDRDAEL"
     gene            complement(1702..2553)
                     /locus_tag="VFP93_01120"
     CDS             complement(1702..2553)
                     /locus_tag="VFP93_01120"
                     /inference="COORDINATES: protein motif:HMM:NF028242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HET9843241.1"
                     /translation="MSKHLFPLTLLGFLTPFLIGCQQQPTTTSHEFTRISEIENVDEK
                     IFSISGLRHYALRDTALSLGARGGLAWRASQIRGEVYRHERKLDLIFNFYGLILDDNV
                     LPPVLIEGRQTLDQPQDDTLRIADRAYVIIQQARFVTAPPNWRDYLKMDYAPPDLPDR
                     SLLPRNAAEKSVWERYIREGWQAGIVQANTIFLENLGRLKRDYEGMIRYHTLLAQGMV
                     TKPFVARMELGITGDEDEMAVNDRILRITVKPSFALNGKWKPEIVQNKSLQLVQSTLV
                     EICPPEQ"
     gene            complement(2742..>3149)
                     /gene="dotD"
                     /locus_tag="VFP93_01125"
     CDS             complement(2742..>3149)
                     /gene="dotD"
                     /locus_tag="VFP93_01125"
                     /inference="COORDINATES: protein motif:HMM:NF033882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system lipoprotein DotD"
                     /protein_id="HET9843242.1"
                     /translation="NIPTVSESLNVDQQLSSAAYSVQESLHALAAAKEPHELNAINTE
                     LLVTPEGGMGNPVTIDWSGPLEPLLYRLGGLSHYSVKVLGPSPAIPIIVTMHVENRRV
                     ADILKDAGLQAGNRANIVVYPESKIIELRYVPT"
BASE COUNT          930 a          687 c          606 g          926 t
ORIGIN      
        1 agaaggtcgg gtataaatca aaaaaattat aattcctgcc aacacaatta tggcagtaat
       61 cataagaaga caagcgacca tgagtctgcg aaaattatcg cgataaaaat tatttcgcaa
      121 taataccatt tctaaacccg aagattctcg gggatgcccg gtttttactg gccgtttcgc
      181 gctcgtcggg tgcgaagttt ttttcccttc agcaggcggc acctcagtta ccatggttta
      241 acctcttttt tatgcaaacg aaaattaacc tctccaagtt ttatgcagta cccaccaagg
      301 acgtgcaatt cgggtattac ctgctaaaaa tgcacgtatc catctttgga agactggaac
      361 cgtaaaccca aatttttcca aaatcacgaa aaaagcgata gccgcgatac agagaaaaaa
      421 agtccaccat cttatatgca ataaaaataa aagcaaagga aaagcagaat aggcatccat
      481 aaaaaataat tttggtcttc tcgccgaatc tcgccaatgt gctgtagcag gatttctcat
      541 actatctctt ctttataact ctgcatctcg atccaatttt ttggcactcg cagctaatat
      601 cttatagaca cgttcttcaa tgataccttc ttcaaatttt ttttgagcat ctacaatcat
      661 cggttgtcca cgctccttta atagaagccg tgttttagct gctaattttt ccacatccgt
      721 cataacgaga atatctcgaa cttcttcatc aaaaaccaaa tattctctta aagcgacccg
      781 ttttccttct ttggtaggta caagtttttg ccaaatcacc aaacgtaatg tttctaaaat
      841 atccaaagca cgtccatgcc gctcacctgc aggaaaagta gaaaccatac gacgaacaca
      901 atccgcaact ccattactgt gaagcgttgt atatacagga tgtcctgtta aagctgcatc
      961 aattaccgca gcgatagttt ctacatcgcg ggcctcgccc acaagaatga gacctggctt
     1021 tctgcgtaat gcattccgaa cgccggccgc aaaagaaggt agatggcgcg ggatctctga
     1081 ttgggcaaca atcgagcttg gcttttttac agaatcatat acaaactcaa tgggcgcttc
     1141 atacgttaaa accttgcggt tagcatcgag ttgactggta atttctttaa taattgaagc
     1201 taataaagta ctttttccgg aacctgtggc gcctgttacc actacagtac cttgcacagg
     1261 tgccatactg tctaaaatag cctggtccaa atgcatactt ttcaaatccg gaggttcgga
     1321 aggtatcgtg cgcgctgtta tttgaatagc atcacttcct tctaccatgc aaccggtacc
     1381 atttatacga aatcgaaaac gtgtttctcg atcaggtttt atttcgtaat gggtgtcaac
     1441 atcctgacca gaagaaattt gggtagtcgc gttgggtcca taaatagcat ttaacatatc
     1501 agcaacttcg gcggcagaaa tttttctgga tgttaccgga taaagtttgc catgaatctc
     1561 ggccaaaatt tcagatttgg attgcagcgt tacatcggat gcgcccaatt ttgtacaatg
     1621 taaaagtaaa cgatccatat cgcgtggtgt gaagcgaatg ggctcttcgg gtaataaaag
     1681 ttcttttgac aattcaggca tttattgctc cggagggcat atttccacta aggtactttg
     1741 aaccaattgt aaacttttat tctgaacaat ttcaggcttc catttaccat ttaacgcaaa
     1801 agacggttta acggttatac gtaaaatgcg atcgttgacc gccatctcat cttcatcacc
     1861 cgtaatgccc aattccatac gtgcaacaaa gggtttagtg accatgcctt gcgcgagcaa
     1921 cgtatgataa cgaatcattc cttcataatc gcgttttaat ctacctaggt tttctagaaa
     1981 aatcgtattt gcttgcacaa ttccggcttg ccagccttct cgaatgtaac gttcccatac
     2041 acttttttct gccgcattac ggggaagtaa tgaacgatcg ggtaaatccg ggggtgcata
     2101 atccattttt aaataatcgc gccaattcgg cggagcagtc acaaaacgtg cttgttggat
     2161 aatgacgtac gctctatccg caattcttaa agtatcatct tgcggttgat caagcgtttg
     2221 tctaccttcg attaaaacgg gaggtaaaac attatcgtca agaataagtc cataaaaatt
     2281 aaaaataaga tcaagttttc tttcatggcg ataaacttca ccacgtattt ggctcgcgcg
     2341 ccaagcaagc ccgcctcgag ccccaagact taacgcagta tcgcgcaatg cataatggcg
     2401 taaacctgaa atcgaaaaaa ttttttcatc gacattttca atttcgctaa tgcgtgtgaa
     2461 ctcatgagaa gtggttgttg gttgttgctg acagccgata agaaaaggag tcaaaaagcc
     2521 caacaatgtt aagggaaata agtgtttact catagttcaa tcatagcact cattaattgc
     2581 atagtacttg tgaaaattac aacctactgt atagcctata gcataacatc aatcgttctg
     2641 gtaagtgata ttcaaaaaaa agcaatagaa taggttatag ttataaatga cctataacct
     2701 tccgttggta tttggtattt atgaggatta gttattttaa gttaggttgg tacatacctt
     2761 aattctataa ttttactttc tggataaacc acgatattgg cgcgatttcc cgcctgcaaa
     2821 cctgcatctt tcaagatatc tgctacgcgt cgattttcga cgtgcatagt aacaataatg
     2881 gggattgctg gagagggacc taacactttc acactataat ggcttaaccc acccaagcgg
     2941 tataaaaggg gttcgagagg tccactccaa tctatggtta ccggatttcc cattccacct
     3001 tcgggagtta ccagtaactc cgtgttgatg gcatttaact catgtggttc ttttgctgca
     3061 gccaaggcat gtaaagattc ttgcaccgag tatgctgcac tacttaattg ttggtctacg
     3121 tttaaacttt ctgagacggt cggaatatt
//
LOCUS       DASTIB010000068         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_292473, whole genome shotgun sequence.
ACCESSION   DASTIB010000068 DASTIB010000000
VERSION     DASTIB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292473"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFP93_01130"
     CDS             <1..1140
                     /locus_tag="VFP93_01130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013660651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET9843243.1"
                     /translation="ALSIIFFSCLCQSMAFARVPSPPQTNAKSYILVDFHSGQILASQ
                     NEHEKMDLASLTKIMTVYVVDQELEAGRIKMGDMVHISDTAWRAQGSRMFVEVNTDVS
                     VQDLIRGVIIQSGNDASIALAEHVAGSEESFVNLMNQTAQRLGMKNTHFTNATGLPHP
                     EHYSTAYDISLLARATIHDFPESYKLHAEKWFTYQDIKQPNRNRLLWRENFVDGIKTG
                     FSSTAGYCLAASGLQNGMRLISVVLGAESDGARTDFSQSLLRYGFRFFETHQLFNANK
                     PIKEAKVWMGKSQKLPLGITGDVYITVPQGEYEKLNAQVSIDKKLHAPIKKGEIKGKL
                     TVKLENKLLIEKSLVSLQSVEAGGMWSRFSDYISLKIHDWFKSEA"
     gene            1140..1421
                     /locus_tag="VFP93_01135"
     CDS             1140..1421
                     /locus_tag="VFP93_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HET9843244.1"
                     /translation="MAESEDQNRETLLKFPCDYTIKIIGNTSQEFEAEVISIVRKHFP
                     NIGEGAVTQKLSKESKYLAMSVSLWVENQKSLDNLYKELTSSPFTIFVL"
     gene            1424..2071
                     /gene="lipB"
                     /locus_tag="VFP93_01140"
     CDS             1424..2071
                     /gene="lipB"
                     /locus_tag="VFP93_01140"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET9843245.1"
                     /translation="MVTIRYRSFFDLQAYETTFEQMRSFTLERQEDTVDEIWFLEHHS
                     VYTQGQAGKPEHILQSNTIPIVQSDRGGQITYHGPGQLMTYCLLDLKRRNLGARSLVC
                     ALENSFIELLDSYNLQGITRKNAPGVYLKENGAKIASIGLRIRKGYSYHGVSFNLTMD
                     LSPFKAIRPCGIEDLTLAQLSSYVPDVTRADVETRLQRILANHLVPQTKLCMEYS"
     gene            2068..3021
                     /gene="lipA"
                     /locus_tag="VFP93_01145"
     CDS             2068..3021
                     /gene="lipA"
                     /locus_tag="VFP93_01145"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9843246.1"
                     /translation="MSLDDPTQKLKGEEKFKRLPTKIRPQQEQERQRLPAWIRIRAPI
                     DNSIAQLKGLLREHKLHTVCEEASCPNLSECFSHGTATVMIAGDKCTRRCTFCDVAHG
                     RPDPLDPQEPKRLAEAIAKLHLKYIVITSVDRDDLRDGGASHFAECIKEVRELSPQTK
                     IEVLVPDFRGRMDLALEKLQIALPDVFNHNLETIPALYPTVRPGSDYKWSLNLIKQFK
                     QLNPSIPTKSGLMLGLGEDNAQIIEVLHELRNHDCDWLTLGQYLQPSKFHHPVKRYVH
                     PDEFVELKQVALDLGFTHVASGPLVRSSYHADLQARGEVVK"
BASE COUNT         1018 a          623 c          652 g          938 t
ORIGIN      
        1 gcattgagta tcattttctt tagttgtttg tgccagtcta tggcgtttgc ccgtgtgccc
       61 tccccaccac aaactaacgc caaatcatat attttagttg actttcacag tggacagatt
      121 cttgctagcc aaaatgagca cgaaaaaatg gatttggcta gtcttactaa aataatgact
      181 gtttacgttg ttgatcagga attagaagcc ggtcgcatta aaatgggtga catggtgcac
      241 atcagtgata ccgcatggcg cgcccaagga tcaagaatgt ttgttgaagt aaatacagac
      301 gtttctgtgc aagatctcat acgcggggtg attattcaat ctggaaatga tgcatcgata
      361 gctttagccg aacatgtagc aggttcagaa gaatcttttg taaatttaat gaaccaaact
      421 gcacaacgcc ttggtatgaa aaatacgcat tttacgaatg ctacagggct tcctcacccc
      481 gaacattata gtacagccta tgatattagt ttacttgcgc gcgcaactat tcacgatttt
      541 cctgaaagtt ataaattgca tgcagagaaa tggtttacgt atcaagacat caaacaaccg
      601 aatcgcaatc gtttattgtg gcgtgagaat tttgtcgatg gcattaaaac cgggttttct
      661 tcgaccgcag ggtattgttt agcggcctcg gggttacaaa atggtatgcg tttaatctcc
      721 gttgtattgg gggcagaaag tgacggcgct cgtaccgact tttcgcaaag tttgttgcgt
      781 tatggttttc gattttttga aacacaccaa ctatttaatg ccaacaaacc tataaaagaa
      841 gccaaagttt ggatgggcaa atcgcaaaaa ttgccacttg gaatcacggg cgatgtatat
      901 atcaccgtac cgcaaggtga atacgaaaaa ttaaatgcgc aagtgagcat cgataaaaaa
      961 ttacatgcac ctatcaaaaa aggagaaatc aaagggaagt taaccgttaa attagaaaat
     1021 aaattattaa tcgaaaaatc gttggtttcg ctacaatccg tcgaagcagg tggtatgtgg
     1081 tcaagatttt ctgactatat ctcgcttaaa attcatgatt ggtttaagag tgaagcataa
     1141 tggctgaaag cgaagatcaa aacagggaaa ctttattaaa gtttccctgt gattacacca
     1201 taaaaattat tggtaatact tcacaagaat ttgaagcaga agtaataagc attgtgcgca
     1261 aacattttcc caatatagga gaaggtgcag taacccaaaa attgagcaaa gaaagcaaat
     1321 atttagctat gtctgtttcc ctttgggtgg aaaatcagaa atctttagat aatctctata
     1381 aagaattaac ttccagccca tttaccattt ttgtgttata acgatggtta ctatacgtta
     1441 tcgttctttc tttgatttac aagcgtatga aaccactttt gaacaaatgc gttcttttac
     1501 cctcgaaaga caagaagata cggtagatga aatttggttt ttagaacatc attccgttta
     1561 cacccaaggg caagccggca agcccgaaca tattctacaa agtaatacga ttcccattgt
     1621 gcaaagtgat cgtggaggcc aaattactta ccacggcccc gggcaattaa tgacttattg
     1681 tttgctcgat ttaaaacgac gtaatttagg ggctcgatcc ctggtttgcg cgttagaaaa
     1741 ttcttttatt gaacttttag actcctataa cctgcaagga ataacacgaa aaaatgcacc
     1801 gggagtttat ttgaaagaaa atggcgcaaa gatcgcatcc attggtctgc gcattcgaaa
     1861 aggttatagt taccatgggg ttagttttaa tttaactatg gatttatccc cttttaaggc
     1921 tatccgcccc tgcggaattg aagatctaac cttggcccaa cttagttctt atgttccgga
     1981 tgtcacccgt gcagatgttg aaacccgttt acaacgcatt ttagccaacc atctcgtacc
     2041 gcaaaccaag ttatgcatgg agtattcatg agtctcgatg atccgacaca aaagctaaaa
     2101 ggcgaagaaa aatttaagcg attacccaca aaaatcagac cgcaacaaga gcaagaaaga
     2161 caacgtctgc ctgcttggat tcgtattcga gcgcctatag ataattccat tgcgcaatta
     2221 aaaggcttat tacgagaaca taaattacac acggtttgcg aagaagcttc ttgtcctaac
     2281 ttaagtgaat gttttagcca tggtaccgca acggtcatga tagcaggcga taaatgtacc
     2341 cgtcgttgta ctttttgtga tgtggcacat ggtaggcctg atccactgga tccacaggag
     2401 ccaaaacgat tagcagaagc catagcaaaa ctccatttaa aatatatagt catcacttca
     2461 gtcgacagag atgatctgcg agatggaggt gcttcgcatt ttgccgaatg tattaaagaa
     2521 gttcgagaat taagcccaca aacaaaaata gaagttttgg tacccgattt tcgaggtagg
     2581 atggatcttg ctttagagaa actccaaata gctttgccgg atgtatttaa ccataactta
     2641 gagactattc cagcgcttta tcctaccgtg cgacctggat cggactataa atggtcgctt
     2701 aacctcatta aacaatttaa acaacttaat ccaagtatac cgacaaagtc aggtcttatg
     2761 ttaggtcttg gcgaagataa tgcgcaaatt attgaagtac ttcacgaact caggaatcat
     2821 gattgtgatt ggctaacatt aggacaatat ttgcaaccaa gtaaatttca ccatccggtt
     2881 aaacgttatg tgcatccaga cgaatttgta gagcttaaac aagttgccct ggatctggga
     2941 tttacccatg ttgcttctgg tcctttggtg cgttcttcct atcatgctga tttgcaagca
     3001 cgaggcgagg tggtcaaata aggagttgtc cccataaact gaatacaact tcttgagtaa
     3061 gctgtgaact atttgtaaaa cactaataat aacaacaact tgattgaatt gttcattttt
     3121 tgaacatgat ttataagtat tctttactcc attttggtgt cagctgatga gctattttaa
     3181 gttgatccaa aatttttcca acaacaaaat ctacaagctc agataattgt g
//
LOCUS       DASTIB010000069         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1246429, whole genome shotgun sequence.
ACCESSION   DASTIB010000069 DASTIB010000000
VERSION     DASTIB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246429"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..922)
                     /locus_tag="VFP93_01150"
     CDS             complement(158..922)
                     /locus_tag="VFP93_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843247.1"
                     /translation="MARVKKGSPAAKRGRPGRPTIKAKKVAPKKVVAKKGRKAKTVDL
                     GVALEKDMHNNLDQLVKHWTKKASVLTKETDSLRSKLKKAEAKYNAGEKKYAQLEDKF
                     NENPSKVVEKQLKKALAGQNEASSFIEEIQNAIEGSQNNRELAKSQSEKYSKLVQMIA
                     EFEDDWANQEQEAMEAAQSKAEQAAKESAKGKRGRKSKKEEQPLEEDYSELEEAEEME
                     EVEESLPEVDFDYMEEEAQEQAEEPESDLEFEEEEF"
     gene            1168..1497
                     /gene="fdxA"
                     /locus_tag="VFP93_01155"
     CDS             1168..1497
                     /gene="fdxA"
                     /locus_tag="VFP93_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HET9843248.1"
                     /translation="MTFVVTENCIRCKYTDCVEVCPVDCFREGPNFLVIDPDECIDCA
                     LCAPECPAEAIFSEDELPEDQKEFLAINAEKAKEWPVISTHKEPNVDADQWNGVPNKR
                     EHLSESS"
     gene            complement(1501..1962)
                     /locus_tag="VFP93_01160"
     CDS             complement(1501..1962)
                     /locus_tag="VFP93_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HET9843249.1"
                     /translation="MKTKPTDIQAQFLNSIQQWLNLRQKMIVAFNELCLLKPFEIIKK
                     PEIQTCVDNFCNELVDYLSFGQFKVFEQLSSIDALRTEDRKSIQALWCKIFSTTVTAI
                     EFSDLYTHNLCMATLQKDLSKLGVQLAHRLDWEDQILEILYRTMDFNISPS"
     gene            complement(2461..>2693)
                     /locus_tag="VFP93_01165"
     CDS             complement(2461..>2693)
                     /locus_tag="VFP93_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HET9843250.1"
                     /translation="QSLPPNEAPACGPGFSYLMEVLPLKEALKQAFMGAGDCAQVQGT
                     FLSLSLPSWTFILFTLLAILFLISSILEIKKG"
BASE COUNT          765 a          468 c          515 g          945 t
ORIGIN      
        1 gattgtcagg gatacaagcg ttgttaaagc attatgtttt ctttgtaata aacattgctt
       61 aaatttcacg ccctgccaaa tagcgaacaa aaaacgatac ataattcctg caatagagct
      121 tcccattgga ggtaagctct attttagtta agttttgtta aaattcttct tcttcgaatt
      181 ctaagtcact ttctggttct tctgcttgtt cttgtgcttc ttcttccatg taatcgaaat
      241 caacttcagg aagtgattct tctacttctt ccatttcttc cgcttcttcc agttccgaat
      301 aatcttcttc cagaggttgt tcctcttttt tagatttgcg accacgcttt cctttggcag
      361 actctttggc tgcttgttca gccttggatt gtgcagcttc catagcttcc tgttcttgat
      421 tagcccagtc gtcttcaaat tctgcaatca tttgcacgag tttgctgtat ttctcagatt
      481 gtgatttggc taactctctg ttgttttgtg acccttcaat agcattttga atttcttcga
      541 tgaaagagga agcttcattt tgtccggcaa gcgctttctt taattgcttt tccacaactt
      601 ttgaaggatt ttcattaaat ttatcctcga gttgtgcata ttttttctcg ccagcgttat
      661 attttgcttc tgcttttttc aatttggaac gcaagctatc tgtttctttt gttaagactg
      721 aagctttttt ggtccaatgt tttaccaatt gatccaaatt attgtgcatg tctttttcta
      781 atgctacgcc taaatcaacg gttttggctt tacgtccttt ttttgcaacg acttttttag
      841 gggcaacttt ttttgctttt atggttggac gtcctggacg accacgttta gcagcaggtg
      901 aacctttttt aactctagcc atgctttttc tcctatgcgg gtttttaaaa ttattctaag
      961 aaatgaatta tacaaaaaaa aattaaaaat taattttata tttgtaattt ttttagaaat
     1021 ttataattgc cttaaataaa aaggatttta aagatccttt tttgaaaaag ctaggttgat
     1081 ggctgcattt gtgttattat tagcggtctt taagcgctaa cttactttta gcatgtttaa
     1141 tctgaactta agacttagag ggtattcatg acttttgtcg tcacagagaa ttgtattcgt
     1201 tgtaaatata ccgactgtgt tgaagtgtgc ccagtagatt gttttcgcga gggaccgaat
     1261 tttttagtta ttgatcctga tgaatgcatt gattgtgcgt tatgtgcacc ggaatgtcca
     1321 gcggaggcca ttttttcaga agacgaatta ccagaggatc aaaaagagtt tttagcgatt
     1381 aatgccgaaa aagctaaaga atggccggtc atttctaccc acaaagagcc gaacgtagat
     1441 gcggaccaat ggaatggagt gcctaacaaa agagaacatt tgtccgaatc tagctaatta
     1501 ttaggaaggt gagatattaa aatccatagt tctataaagg atctctaata tttgatcttc
     1561 ccagtccaag cgatgagcaa gttgcacacc taattttgat aaatcctttt gtaaggtcgc
     1621 catgcataaa ttatgggtat acaaatcact aaattcgatt gccgtgaccg ttgttgaaaa
     1681 aattttgcac cataatgctt gaatcgattt tctgtcctca gttcttaaag catcgataga
     1741 acttaactgc tcaaaaacct taaattgtcc aaaactaaga taatccacca gttcattaca
     1801 aaaattatca acgcaagttt gaatttccgg ttttttaatt atttcaaagg gttttaacaa
     1861 acaaagttca ttaaacgcaa caatcatttt ttggcgtaga tttagccact gctgaatact
     1921 atttagaaac tgtgcttgta tatctgttgg ttttgttttc ataaaaaaaa atttgatctt
     1981 taacttaagt aaagcttcta aaataatatt gtcaaggtat gttggaaggt gagatcctta
     2041 tggtgtgtgt tgtgcgacat ttcttaaatc catgtcaatc gagtgctcat tcaagagagc
     2101 gttttttaga aagatgctgt gatctgtttt taagtgattg ttatataaga aaaaatttcg
     2161 acaataattt tttggaggtt acagttgaca actataaaaa tcaaaaagtg ctgcttgttt
     2221 aagcaaaatg atttgttaaa gtgaaggtat cccctatcaa cggagcgata gaggacccag
     2281 ctcgttacag gatgtaacaa cacatgggca agggattgcc gcctcttttg gggtttgatc
     2341 aaggaatact ggactttagg caacggaaag ccacttagtt caaaggattg gattaagaaa
     2401 gtctggttca gtgtggggct ggtgggttga tagcacggag tgctgagggt agcaaatttg
     2461 ctaccctttt tttatctcta aaatactact gattaaaaat aaaatcgcta aaagggtaaa
     2521 taagataaaa gtccagctcg gaagtgataa gctaagaaaa gttccttgga cttgagcaca
     2581 atctccggca cccataaatg cttgtttaag tgcttctttt aagggcaaga cttccatcag
     2641 ataagaaaat cccggaccac aagcgggtgc ttcattggga ggtagggatt gca
//
LOCUS       DASTIB010000070         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_784229, whole genome shotgun sequence.
ACCESSION   DASTIB010000070 DASTIB010000000
VERSION     DASTIB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784229"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1688
                     /locus_tag="VFP93_01170"
     CDS             180..1688
                     /locus_tag="VFP93_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HET9843251.1"
                     /translation="MLKCQAILHVLRKIVGLIMSDTQLKETQNSNIRYKAMLVCILVA
                     LCIWFSPKPEAVPPEGWHLLAIFITTILALILKPLPMGAVALIAMLAAIFTNTLAPAV
                     VFGAFQDTSVWLVVLALFIAKGFAVTGLGKRIAYFFSYLLGRSSLGLGYGLITTDLLI
                     APATPSVTGRVAGIVLPILKGIANSYQSFPASSSSRRIGGFLTLVAFQGTAVTSSMFL
                     TAMAANPILHQFTQAQGLELSWGKWALAGIVPGLINLALIPLVIYWLYPPEIKYTPDA
                     PMVAKEQLKLLGKVHRQEWIMIFTILLLLVAWIFGAKIQTITGIPLTAVVAGLMGVCI
                     LLLTGVLNWQTLIAMDSVWDTFVWFCVLMMLAGNLNTLGVVGWFSQFVGNQFGGLNWQ
                     IAFPILALIYFYSHYFFASSTAHVTSLFTPFLALAISLQTPPMLAALVLIFFSNLYGG
                     ITHYSLTPAPILYGEGYVDIKDWWRVGFIVSVLNIVVWSTIGVLWWKFLGFW"
     gene            1761..2288
                     /locus_tag="VFP93_01175"
     CDS             1761..2288
                     /locus_tag="VFP93_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843252.1"
                     /translation="MTSLKIDLPDDVYTDLTEVAKRLKQTPEETTQMALSLLMQFESL
                     DYALVAMQRVEDGQETVPLPALAEESELSLEFHPEALDELQTLDEEDQVTVLTELIER
                     IVAEEDELEDTLDLVINENSAEQIVLSSFEFGDIIYRIANKVTVYMIALPTQEEFEAE
                     DISEDEDEEEDEELH"
BASE COUNT          623 a          413 c          489 g          763 t
ORIGIN      
        1 gatggcaaaa gtgcgacgtt ttttaatttc ttctataaat tcagacactt aactacccgt
       61 ataaagacaa attacaatat tggcgcatta gtatcataac tagcagatta tggcaaacgc
      121 atcgttagag atacatcgtt acacttttcc gataatactt ttcgcgctat ggattgttta
      181 tgttaaagtg ccaggcaatt ttgcacgtgt tacgtaaaat tgtagggtta attatgagcg
      241 atacacagct taaagaaacg cagaattcaa atattcgtta taaagcaatg ctggtttgta
      301 tcctagttgc cctatgcatt tggttttcgc ctaaacctga agccgttccc ccagaaggct
      361 ggcatttgtt agccattttt attacaacaa tattagcctt aattttaaaa ccgctgccta
      421 tgggcgctgt agcgctcatt gctatgttag cagctatttt tacgaacact ttagcacctg
      481 ctgtggtttt tggtgctttc caagatactt ccgtgtggct tgtagtatta gccttgttta
      541 ttgccaaggg ctttgctgta acgggcttag gcaaacgtat tgcttacttt tttagttatt
      601 tattgggacg cagctctttg gggctaggtt atggtttaat cactaccgat ttgctcattg
      661 ctcctgcaac acccagtgtc acaggacgcg tggcaggcat tgtattaccc atattaaaag
      721 gaattgcaaa ctcttatcaa agctttcctg cgagcagttc aagtcgacgc attggtggtt
      781 ttttaacgtt ggttgcgttt caaggcaccg cagttacttc aagtatgttt cttacggcta
      841 tggctgcaaa tcccattttg catcagttta cgcaagcgca aggtctagaa ctttcgtggg
      901 gtaaatgggc tttggctgga atcgtacccg gattgataaa tttagcttta atacctttag
      961 taatttattg gctctatccc cccgagatta agtatacacc tgatgctccg atggtcgcta
     1021 aagagcaatt aaaactttta ggaaaagtgc atcgacaaga atggattatg attttcacta
     1081 ttttgctttt attggtagcc tggatttttg gtgcgaaaat tcaaacaatt acggggatcc
     1141 cattaactgc cgttgtcgct ggattaatgg gtgtttgtat tttactactg actggcgttt
     1201 taaactggca aacccttatt gccatggata gcgtgtggga tacttttgtt tggttttgtg
     1261 tgcttatgat gttagcggga aatctcaaca ctttaggggt cgtaggttgg tttagtcaat
     1321 tcgtgggtaa tcagtttggc ggtttaaatt ggcaaattgc ttttcctatt ttagcgttga
     1381 tttattttta ttcgcattat ttctttgcaa gctccaccgc gcatgtaacg tcgttattta
     1441 cacctttttt agctttggca atttctttgc aaacaccgcc tatgttagca gctctcgtat
     1501 tgatcttttt tagtaacttg tatggtggaa tcacgcatta cagtttaact cctgcaccta
     1561 tcttatatgg agaaggttat gtggatatta aagattggtg gagggttggt tttattgtta
     1621 gtgtactcaa tattgtagta tggtccacga ttggcgtttt atggtggaag ttcctaggat
     1681 tttggtagac aatagacaag ctctgttcgg gcgcatacat tttggctcaa gcagttttta
     1741 aaatttaaag aggatatgtg atgactagct taaaaatcga tttgccggat gatgtttaca
     1801 ctgatcttac tgaagtggca aaacgactaa aacaaactcc cgaagaaaca acacaaatgg
     1861 ctttaagcct cctcatgcaa tttgaatctt tagattacgc tttagtggct atgcaacgcg
     1921 tagaagacgg acaagaaaca gttcccttac ctgctcttgc ggaagaatca gaattatcct
     1981 tagagtttca tcctgaagcg ttagatgaat tacaaacctt ggatgaagaa gatcaagtta
     2041 ccgtgttaac cgaacttatc gaaagaatcg ttgccgaaga agatgagtta gaagatacct
     2101 tagacttggt aatcaatgaa aattctgccg aacaaattgt tctatccagt tttgagtttg
     2161 gcgatattat ctacagaatt gccaacaaag ttacggttta catgattgcg cttccaacac
     2221 aagaagaatt tgaagcggaa gatatttctg aagatgaaga tgaagaagaa gacgaagaac
     2281 ttcattaa
//
LOCUS       DASTIB010000071         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_187636, whole genome shotgun sequence.
ACCESSION   DASTIB010000071 DASTIB010000000
VERSION     DASTIB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187636"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFP93_01180"
     CDS             complement(<1..1073)
                     /locus_tag="VFP93_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9843253.1"
                     /translation="MANLTWAFNMTATHAQKHFDMIIVGGGVVGLTLALCLARENFTI
                     AIVEKQKWCFDNLIEEYDTRVFAIHDTSLRILDNLGLLQKMRALRSSPFYKIAVWDKE
                     GDGFIEFDNRLALHLPMGEILEQKVLLYALYTSLKNQTNVTFFEEAAIIEIHSKQAHL
                     RLPNILLTADWMLACDGAQSAVREAAMIPWQRYSYQQQAIIATLACEKNHGQTARQAF
                     FSKGTLALLPLKDSNKISLVWSWEERDSHRLMQLSAQDFVQELAHKMDGCLGHFQLLG
                     KRFTYPLYKAQAAKYWQHRCILVGDAAHVIHPLAGQGINLGILDAASLRDIFVEWRNL
                     KKLSSKRLLNYYQRHRRGHSTFL"
     gene            complement(1048..2187)
                     /locus_tag="VFP93_01185"
     CDS             complement(1048..2187)
                     /locus_tag="VFP93_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9843254.1"
                     /translation="MKEVVILGGGVVGGLLALKLHLLEFQVTLIERRSLAGIFAITTS
                     RTFALNAKSANILRYWDVWPAQAQENPIVEVHIAQHGSFVQSKLCAKDYNLKALGYSI
                     PQHILEQYIYQSLAQCHIKILENCEIIALKEEKEDWQLTCQQQEKVFNLTASFLIVAD
                     GQHSTTREMLHLPAHTVDFPQKAWVGEAQLTQSHNHIAYELFTEQGAVALLPLSSDRA
                     TIVWTLPLEEDHTMQREQALAHLGDYLARFKVELKSFKGLANFPLSCTYAPQQVIKNA
                     ILMGNAAHSLHPIAAQGLNVSIADVATLLHLLEERREEKLQDILEQYQKQRLPFQQGK
                     IWYTNFLAHWIASDKLPTWVKHVSLGGFELFTPLKHIFGQFNMGF"
     gene            complement(2184..>3139)
                     /locus_tag="VFP93_01190"
     CDS             complement(2184..>3139)
                     /locus_tag="VFP93_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HET9843255.1"
                     /translation="LGTETLYYPLCEQTELETTLKKAIKILQTQKRKGYLIPRQHIDS
                     SSVLDALRLIKMPDEIALLEQAATISAHAHIRGMQTCKPEMVEYELEAEYRYEFLRRG
                     AQDVAYTPIVASGPNACILHYINNNRKMRTNELVLVDAGCEYKGYASDITRTYPVSGK
                     FTKDQQAVYEIVLAAQEAAIQVATPSYTWEKMQEAIVAILVQGLVDLKILKGDANYLI
                     EQGAYKPYYMHQSGHWLGLDVHDAGDYKVHDAWRQLEPGMVLTIEPGLYFPQEDQTLP
                     EKYRGLGIRIEDDILITAKEPQVLSQEVPKTVREIEQLMRA"
BASE COUNT          893 a          687 c          579 g          980 t
ORIGIN      
        1 attaaaaaag tactatgccc acgtcgatga cgctgataat aattcaataa tcgtttgcta
       61 gacaattttt tcaaatttct ccactctaca aaaatatcgc gtaaactagc ggcgtctaaa
      121 atacctaaat taattccttg ccccgctaaa ggatgaatca catgtgctgc atcacccacc
      181 aagatgcagc gatgttgcca atattttgct gcttgtgctt tataaagcgg ataagtaaat
      241 cgtttgccca aaagttgaaa gtgccctaaa cacccatcca tcttatgtgc cagttcttgc
      301 acaaaatctt gtgcactcag ctgcattagc cgatgactat cacgctcttc ccaagaccaa
      361 accaaagaaa ttttgtttga atctttgagc gggagtaaag ctaaggttcc ttttgaaaaa
      421 aaagcttgtc gtgccgtttg accatgattt ttttcacagg ctaatgtggc tattatcgct
      481 tgctgctggt aggagtaacg ctgccatgga atcatagccg cctctcttac cgcagactga
      541 gcaccatcac aagcaagcat ccaatcggca gttaacaaaa tgtttggtag gcgtaaatgc
      601 gcttgcttac tatggatctc tatgatggca gcttcttcaa agaatgttac attcgtttga
      661 ttctttaggg atgtataaag cgcatataat aaaacttttt gctctaagat ttcgcccatg
      721 ggcaagtgta gcgccaaacg attatcgaat tctataaaac catcgccttc tttgtcccat
      781 accgcaattt tataaaaagg gctggaacgt aacgctcgca ttttttgtaa aagccccaaa
      841 ttatcaagaa ttcgtaaaga tgtatcatga atggcaaaaa cacgagtatc atattcctca
      901 attagattat cgaaacacca tttttgcttt tctacgatag caatcgtgaa attttctcgc
      961 gctaaacaaa gtgctaacgt cagacctacc acaccaccac ccacgatgat catatcaaaa
     1021 tgcttttgcg catgcgttgc ggtcatatta aaagcccatg ttaaattggc caaaaatatg
     1081 cttcaagggt gtaaacaatt cgaatccacc tagactcaca tgcttaaccc aggttggtaa
     1141 tttatcactt gctatccaat gggctaaaaa attcgtgtac catatttttc cttgttgaaa
     1201 aggtagtctt tgtttttgat attgttcaag aatatcttgc aatttttctt ctcttctctc
     1261 ttccagtaga tggagcaaag tagctacatc ggcgatactc acatttaatc cttgggccgc
     1321 aataggatgc aaactgtgcg ctgcatttcc catcaagatc gcgtttttta taacttgctg
     1381 aggtgcatac gtacaactga gaggaaaatt tgctaaccct ttaaaacttt ttaattcaac
     1441 tttgaatctt gctaagtaat ctcccaaatg cgcaagtgct tgttctcgtt gcatcgtatg
     1501 atcttcttct aaaggcaaag tccaaacaat ggttgcacga tccgaactta ggggtaagag
     1561 agccacagcg ccctgctcgg taaaaagttc ataggcaata tggttatgcg attgggttaa
     1621 ttgtgcttct cccacccatg ctttttgagg aaaatctacg gtgtgcgcag gaagatgcag
     1681 catctctcgt gtggtagagt gctgtccatc tgctacgata agaaacgaag ctgtaagatt
     1741 aaacactttt tcttgttgtt gacacgttaa ttgccaatct tctttttctt cttttaatgc
     1801 aataatttcg cagttttcaa ggatcttaat gtgacattga gctaaagatt ggtaaatgta
     1861 ctgttctaat atatgttgcg gaatactata tcctaaagct tttaagttat aatctttcgc
     1921 acacaattta ctttgcacaa atgaaccatg ctgtgcaatg tgtacttcaa caatcggatt
     1981 ctcttgcgcc tgcgcaggcc atacatccca atagcgtaaa atattggcgc ttttcgcgtt
     2041 taatgcaaac gttctactag ttgtaatagc aaaaataccc gcaagcgagc ggcgctctat
     2101 taaagttacc tgaaattcta acaagtgcaa ttttaaggca agaaggccac caacaacccc
     2161 tccccctagg atcactactt ctttcatgcg cgcatgagct gttcaatttc gcgtacggtt
     2221 ttgggtacct cctgcgataa aacttgcggc tccttagcag taatgagaat atcatcttca
     2281 atgcgaatgc ctaaaccgcg atatttttct ggcaaagttt gatcttcttg cggaaagtaa
     2341 agcccaggct ctatggtaag caccatgcct ggctctaatt gcctccatgc atcatgtact
     2401 ttatagtcac cagcatcatg gacatctaaa cccaaccaat gaccactctg atgcatataa
     2461 tagggcttat aggcaccttg ctcgattaaa taatttgcat cacctttaag aatttttaag
     2521 tcaactaatc cttgaacaag aatggcaact atcgcttctt gcattttctc ccaagtataa
     2581 gatggagttg ctacttgtat tgctgcttct tgcgctgcca gaacaatctc gtataccgct
     2641 tgttgatctt tcgtaaattt tccactgact ggataagtcc gcgtgatgtc ggaagcgtat
     2701 cctttatatt cacaaccagc atctactaat actaattcgt tcgtgcgcat ttttcgattg
     2761 ttatttatat aatgtaaaat gcaagcattc ggcccacttg ccacaatggg cgtataagct
     2821 acgtcttgcg caccacgacg taaaaattcg tatcggtact ctgcttccaa ttcgtattca
     2881 accatttcgg gtttacaggt ttgcatgcca cggatatggg catgcgccga aatagtggca
     2941 gcttgttcca ataaagcaat ttcatctggc atttttataa gccgcaaagc atctaataca
     3001 ctgctagaat cgatatgttg tctgggaatc aaatagcctt tacgtttttg cgtttgtaaa
     3061 attttaattg ctttttttaa tgttgtttca agctcggttt gttcacataa aggatagtaa
     3121 agcgtttctg ttccaagta
//
LOCUS       DASTIB010000072         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1321047, whole genome shotgun sequence.
ACCESSION   DASTIB010000072 DASTIB010000000
VERSION     DASTIB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321047"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="purH"
                     /locus_tag="VFP93_01195"
     CDS             complement(<1..1548)
                     /gene="purH"
                     /locus_tag="VFP93_01195"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET9843256.1"
                     /translation="MSITIQRALLSVYDKNDLLPFATFLHQRGIEIVSTGGTALLLKK
                     HNIPVIEIEDFTQHPEIMEGRVKTLHPKIHGGILGRRDKDVAIMEQCGISDIDLIVVN
                     LYPFEKVIQEPTCTLEHAIENIDIGGPALIRAAAKNHAWVTVVVDPKDYVLIMQQLEQ
                     ENSIAEHVRFRMAKKAFHTTAKYDGCIANYLSKLDDEYHPKAFGDYCHIPLEFDRSLR
                     YGENPHQIAALYREITPNQTTLSHAEFLHGKGLSHNNILDAETAITCCYQFENPTCVI
                     VKHANPCGVAESTTLLSAYEKAFLCDPQSAFGGILAFNRAVDKDLLAKIFSQQFVEVV
                     IAPEFTRDALHFAKLKPNTLLIMTGTTKLDFSHLLPDFKKIRGAVLLQGWDNIPFDPS
                     ACSIVTKKSPTATQWEDLIFAWKVVKFVKSNAIVLAHDRHTIGIGAGQTSRVSSVEIA
                     RDKTKISTVKTDKAVLASDAFFPFKDGVELAANIPITAIIQPGGSIRDQEVIDTCDQL
                     GLAMVFTH"
     gene            complement(1560..1811)
                     /locus_tag="VFP93_01200"
     CDS             complement(1560..1811)
                     /locus_tag="VFP93_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9843257.1"
                     /translation="MSKTTTHPPLCEFVMTTLSHYFSHLEGEKPTNLYNMVIKEVEKG
                     FLKVVMQEARGNQCLAAKMCGMSRNTLRKKLKEHQLDKI"
     gene            complement(1804..2787)
                     /gene="dusB"
                     /locus_tag="VFP93_01205"
     CDS             complement(1804..2787)
                     /gene="dusB"
                     /locus_tag="VFP93_01205"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET9843258.1"
                     /translation="MRPLKIGSHIIEHPFILAPMAGVTDRPFRQMCKKLGAGLAVTEM
                     VGAGSFLHGSDKTHRRANHEGETRPCSVQIVGADPKMMAEVAKRNAELGADIIDINMG
                     CPAKKVCNTLAGSALLKDELLVGDILESVVNAVNIPVTLKIRTGWDVAHRNGVKIAQI
                     AQNAGIQALAIHGRTRACMFKGEAEYETIRAIKAAVNIPIIANGDIDSPQKALLVLEN
                     TQADGIMIGRAAQGMPWIFREIIHFFKNGILAKAPTLGEIRQTLTEHIENLYAFYGEH
                     RGMLVARKHVSWYTKGQRQGASFRQHFNQLTCAKEQRIAIDRFFDERMEHE"
     gene            complement(2876..3757)
                     /gene="prmA"
                     /locus_tag="VFP93_01210"
     CDS             complement(2876..3757)
                     /gene="prmA"
                     /locus_tag="VFP93_01210"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9843259.1"
                     /translation="MSWIEVAFELEASKIEAGINLLEEAGALAISIYDAKDSPIFEPP
                     PKSEPILWPYSILVGLFNAEFPVHTLLDSVAAIFPTASAKVRNVRDQNWVAICQAHFP
                     ATRFGNLWICPSWANKPTDPSSLCLNLDPGMAFGTGSHATTALCLQWISQTHFQTKKV
                     LDYGCGSGILALACALSGALRVWGVDYDAQALASSKTNQANNKIPETQLSFHEPEDTT
                     VQSIQVDILIANILVTPLVELKPTFSRLLKPYGTLVLSGILANQQEIIESAYSDAFDF
                     IEKTSKEDWICLVAQKK"
     gene            complement(3757..>4030)
                     /locus_tag="VFP93_01215"
     CDS             complement(3757..>4030)
                     /locus_tag="VFP93_01215"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841032.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9843260.1"
                     /translation="VHVPGGPGIRFDSHIYNTYVIPPNYDSLIGKLIAWGENRLEAIA
                     RLRNALDEIVIEGIKTNIPLHQELLRDANFIQGGTTIHYLENKLKN"
BASE COUNT         1142 a          838 c          781 g         1269 t
ORIGIN      
        1 gtgggtaaaa accatagcaa gaccaagttg atcacaagtg tctatcactt cttgatcgcg
       61 aatagaacca cctggctgaa taatagcagt tatcggaata tttgcagcca attccacccc
      121 atctttgaat ggaaaaaaag cgtcacttgc taataccgct ttatcagtct ttactgtgga
      181 aatttttgtt ttgtcacgcg ctatttctac actcgacacg cgagaggttt ggcccgcgcc
      241 aattccaatg gtatgccgat catgtgctaa tacgattgca ttcgatttga caaatttaac
      301 aactttccaa gcaaaaatga gatcttccca ttgggtcgcg gtaggtgatt tttttgttac
      361 aatggaacat gcagatggat caaaaggaat attgtcccaa ccttgtagta gaacagctcc
      421 tcgaattttt ttaaaatcag gcaataagtg cgagaaatca agcttagtgg taccggtcat
      481 gatgagcaaa gtgtttggtt ttaatttggc gaagtgcaga gcatcgcgcg taaattctgg
      541 tgcaatcacc acttctacaa actgttgtga aaatattttt gcaagtaaat ctttatccac
      601 cgccctgttg aaagcaagga ttccgccaaa tgcagattgt ggatcgcaaa gaaatgcttt
      661 ctcatacgcg gataaaagcg tggtactttc tgccacccca cacggattgg catgtttaac
      721 tatgacacag gtaggatttt caaattggta acaacatgta atagccgttt ctgcatctaa
      781 tatattattg tgggataaac ctttaccgtg caagaattct gcatgtgaca atgtggtttg
      841 attaggcgtt atttcacgat atagagctgc tatttgatgt ggattttctc catagcgtaa
      901 acttctgtca aattctaaag gaatatggca ataatctcca aaagcttttg gatggtattc
      961 atcatctaat ttgcttaaat aattagcaat acacccgtcg tatttggctg tggtgtgaaa
     1021 cgcttttttt gccatgcgaa atcgcacatg ttccgcaatg ctgttttctt gttctaattg
     1081 ttgcatgata agcacataat cttttggatc cactacaacg gtaacccatg catgattttt
     1141 agcggctgcc cgaataagcg caggaccacc aatatcaata ttttcgattg catgttctaa
     1201 ggtacaggtt ggttcttgaa taactttttc aaaagggtaa agatttacga caatcaaatc
     1261 gatatcagaa attccacatt gctccataat tgcaacatct ttatcacgcc gtcctaaaat
     1321 tccgccatgt atttttggat gcaaggtttt cacacgaccc tccatgattt caggatgctg
     1381 cgtaaaatcc tcgatttcaa tgactgggat gttgtgcttt tttaataaaa gcgccgtgcc
     1441 tccggtggat acaatttcta tgccacgttg atgcaagaaa gtggcaaaag gaagaagatc
     1501 atttttatcg tatacgctta gcagcgcacg ctgaattgtt atggacattt tacttgatcc
     1561 taaatcttat caagttgatg ttcttttaat ttttttcgta aggtatttct actcatgcca
     1621 cacattttag cggcgaggca ttggttacca cgcgcttctt gcattaccac ttttaaaaaa
     1681 cctttttcta cttccttgat caccatatta tataaattgg taggtttctc accctctaaa
     1741 tggctaaaat aatgtgataa cgtagtcatt acaaattcgc ataaaggtgg atgggttgtt
     1801 gttttactca tgttccatgc gttcgtcaaa aaagcgatct atggctattc tttgttcttt
     1861 ggcacacgtt agttgattga agtgctgtcg aaagcttgct ccttgtcttt gccctttggt
     1921 ataccaagac acatgtttgc gcgccactag cattccgcgg tgttcaccat aaaaggcgta
     1981 taaattttca atatgttctg tcaatgtttg tcgtatttct ccaagtgtgg gtgcctttgc
     2041 tagtattcca ttcttaaaaa aatgaataat ttctcgaaag atccatggca taccttgagc
     2101 tgctcgacct atcataatgc catcggcttg tgtgttttct aaaacaagta aggctttttg
     2161 cggtgaatca atatccccat tagcaataat gggaatgtta acggcggctt taatggcacg
     2221 aatagtttcg tattctgcct cacctttgaa catacaagcg cgggtcctac catgaattgc
     2281 taaagcttgt attcctgcat tttgcgcaat ttgcgctatt ttcacaccat ttcgatgcgc
     2341 aacatcccaa cctgtgcgta tttttaatgt tacagggatg ttcacagcat tcactacgga
     2401 ttcaagaata tcgcccacca aaagctcatc tttaagaagt gctgaacctg ctaacgtatt
     2461 acacactttt ttagcaggac aacccatatt aatatcgata atatccgcgc ctaattcagc
     2521 atttcttttg gcaacctctg ccatcatttt tggatctgca cctacaatct gtactgagca
     2581 cgggcgcgtt tcgccttcat ggttagcacg acgatgggtt ttatcactac catgcagaaa
     2641 cgagcccgct cccaccattt cagtgacagc aagacctgcg ccaagttttt tgcacatttg
     2701 gcgaaatggt ctatcggtca cacctgccat aggtgcaaga ataaatggat gctctattat
     2761 gtgagagcca atttttaaag gtcgcatgat ttagtaaggt aatgtaataa atgataactg
     2821 gtacattata cccagtttgc aattgaaacc gaatcattca tggatattta ataaattact
     2881 ttttttgcgc aacaagacaa atccaatcct ctttcgaagt tttttcaata aaatcaaagg
     2941 catcggagta agccgactct atgatctctt gttgatttgc taaaattcct gataatacca
     3001 gtgtcccgta aggtttaagt aatcgcgaaa aagttggttt taattcgaca agcggtgtta
     3061 ctaaaatatt ggcaatcaga atatccactt gaatggattg tacagtggta tcttccggtt
     3121 catgaaaact taactgcgtt tctggaattt tattgttggc ctggtttgtt ttgctgctag
     3181 ctagtgcttg tgcatcataa tcaactcccc aaacccttag tgcacctgaa agcgcgcaag
     3241 caagcgccaa aattcctgag ccacatccgt aatcgagcac cttttttgtt tgaaaatggg
     3301 tttgactgat ccattgcaga catagcgctg ttgtagcgtg gcttccggta ccaaaagcca
     3361 tgccgggatc taaatttaaa catagagagc ttggatccgt cggcttattg gcccaactag
     3421 gacatatcca taaattgccg aaacgcgtgg caggaaaatg tgcttggcaa attgcaaccc
     3481 agttttgatc acgcacatta cgtacttttg cgcttgctgt tggaaaaata gctgcaaccg
     3541 aatctaataa agtgtgtacg ggaaactctg cattgaataa gcccaccaaa atagaatacg
     3601 gccatagtat aggttcagat ttaggtgggg gttcaaaaat aggcgaatct ttcgcatcat
     3661 aaatgctaat agctaaggcg ccagcttctt ccaataaatt tatacccgct tcaattttcg
     3721 aagcttctaa ttcaaaagct acttcaatcc aactcatcag tttttcaatt tgttttctaa
     3781 ataatgaatt gtggttcctc cttgaataaa gtttgcatct cgcagcaatt cctgatgtaa
     3841 gggaatattg gtttttatcc cttcaataac aatttcatcg agcgcattac gtaatctggc
     3901 aatagcctct aaacgatttt ctccccaagc tatgagttta ccaattaagg aatcataatt
     3961 aggagggatt acataagtat tgtagatgtg cgaatcaaaa cgtatacctg ggccacccgg
     4021 aacatgtacc
//
LOCUS       DASTIB010000073         7282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1196285, whole genome shotgun sequence.
ACCESSION   DASTIB010000073 DASTIB010000000
VERSION     DASTIB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196285"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1862)
                     /locus_tag="VFP93_01220"
     CDS             complement(183..1862)
                     /locus_tag="VFP93_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843261.1"
                     /translation="MTKYSMPSKLKQCFFIALAILFFSLLFSPYHIFLIEKLTADDAS
                     YLSHGFSLGLNFDLKYKNSIHDWVNPITGAPVHPIGPGFLAAPFITIFSLIDKATNAS
                     VLINAYLFPTSWSYFGFVFASSFYFLAGLYLYIRAFAKFALVSSWEHLLLASGLGVIY
                     YVTYRPVMGHAYEFFSLALVTCGTCKLINSHKDNTLYINFVFSFFIAAGILLTLLIRP
                     ANLQVFLLPFIITLTFHTLKEGESPHFFKASKNAFLFGSLWLGLLVIPFISYNWFMFG
                     EIYPTFKTMYGESSSIYLKNIEDSGSVSLSATTLLLIMRLPFIFKVLFSAEFGVLYTS
                     PILLFGLAFLYHGLAKKIASMKKTVLMNLILISVYVGLPLSLVIIWQSYADAYGYRFL
                     FSLYPLAILGYVLWKSNTKNLLNKALHIIIVGLSFFAIMSVMFYGLNDKLKYHVDENA
                     LEDQSNTRVTGYTIAVLQASIHPSSWLTLAAYRMPGFMLINFLEKIPQGLQSNSIIQK
                     GKAKVQNRIPPTRVYMQASIIFFMFFIFLLIVGIFEYKRNALNQTNLKYNE"
     gene            complement(1874..2449)
                     /locus_tag="VFP93_01225"
     CDS             complement(1874..2449)
                     /locus_tag="VFP93_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9843262.1"
                     /translation="MQREAVFQANTIVDQFLLRYRCSRVRQFLRVHPNTQKVLDLGCG
                     KGQLIHWLLKKNLDAYGVDLKAHGNRIIAGNLNKKIPLPDKSFDLVTSLANIEHLEKP
                     EQNLLEIFRILKPGGYLILTTPSLYAKPILEFLAFKLKWIDATEILDHKRYYSKCSLH
                     KALEQAGFTNIRMRYFQMGFNICVWAKKTEE"
     gene            2645..3394
                     /locus_tag="VFP93_01230"
     CDS             2645..3394
                     /locus_tag="VFP93_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9843263.1"
                     /translation="MSYILSVVIPCYNEAKTIEHILKKIRQSPIEGKLEVIVVNDGST
                     ESMKILLETVCKPLIDVLIHHDKNQGKGAALKTGIAHAKGDYVIIQDADLEYDPNDFP
                     QLLAPFLDNRADVVFGSRFIGGECHRVLYFWHSLGNRFLTLLSNMFTNLNLTDMETGY
                     KVFKRSIIQSLPLQEKRFGFEPEVTARIAHIQGIRLYEVGISYYGRTYEEGKKIGWKD
                     GVRAIYCILKYGLFCRLERKPLPQYLRDNDT"
     gene            complement(3396..4463)
                     /gene="gmd"
                     /locus_tag="VFP93_01235"
     CDS             complement(3396..4463)
                     /gene="gmd"
                     /locus_tag="VFP93_01235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9843264.1"
                     /translation="MKSKVALITGITGQDGSYLAEFLLKKNYEVHGIKRRTSLFNTQR
                     IDHTNRKLILHHGDLTDAMSLMRIIQQVQPDEIYNLAAQSHVADSFEEPEYTANTDAL
                     GALRILEAIRIIGLQNKTRFYQASSSELYGLVQETPQRETTPFYPRSPYAVAKLYAYW
                     ITINYREAYNLYACNGILFNHESPVRGETFVTRKISRGLARIKLGLQDCLYLGNLEAK
                     RDWGHARDYVEMQWLMLQQETPEDFVIATGVQHSVRDFVNFAAKFLNIELTWVGSGVE
                     EHGIDKKTGTIIIKVDPKYYRRTEVETLLGDATKAKQKLNWQPKISFPELVREMVEAD
                     LKLAQRDAMCQERGYKVLECI"
     gene            4682..6277
                     /locus_tag="VFP93_01240"
     CDS             4682..6277
                     /locus_tag="VFP93_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9843265.1"
                     /translation="MANHKSPYADLNIAVIGCGNWGKNHIRVLDELGALYAVSDADVW
                     TAAHFSNEYGVPKYTLDKIIADQKIHGVVIATPAATHQEIAIRCLQAGKHVFVEKPIA
                     LTVAEAKKIHEQAKLSRRILMVGHLLHYHAAFQKLKELHAEGHLGQLQYIYSNRLNLG
                     KFRTEENIWWSFAPHDVSMILALVGEMPNTIMAMGANYLMHTVADVTCTHLTFPKGEQ
                     AHIFVSWLHPYKEQKLVVVGEKAMAIFDDGQPWKSKLKLHPYPVEWVDGLPKPSQSDA
                     TLIDIPKSEPLKEEAKHFLDCILHNKTPITDGNESIRVLTVLNAAEYSIEKYQPVYLA
                     EAHNKFQGELANNYFSHETTCIDPGSHIGSETKIWHFSHILPGTEIGKKCSIGQNCCI
                     GPNVRVGNNCKIQNNVSLYEGVVIQDDVFVGPSVVFTNVNAPRAFINRKREFSPTLVK
                     KGATIGANATILCGLTLGEYCFIAAGAVVTKDVQPFALMAGNPARHIGWVSQDGEPLD
                     EHLCCPRSGRQYRLINEKLYEAS"
     gene            6293..>7282
                     /locus_tag="VFP93_01245"
     CDS             6293..>7282
                     /locus_tag="VFP93_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9843266.1"
                     /translation="MTQTHIVQESASFRDPDGFVFQYRNAWYRFVDESYQEHYDHLIH
                     SGLYERLIQERLLLPHQEISEEAFSFSKPCYKILKPSQLEFVSYPYEWCFSQLKEAGI
                     ATLKIQKIAHQFGMCLKDASAFNIQFYQGNPTLIDTLSFHRCTKNTPWIAMGQFYRHF
                     LWPLLAMANIDVTLNKQFLLYPDGFKHETLKPWFGIKTWFKPSILLHVHLHGIFAKKA
                     AIKQATKKDFQLPETYLPILLDRIISLIEKLTLKQQSVWKDYYHNTNYEPRSFKEKEL
                     HVVNFIQQNRGKALIDFGANNGHFSILALKTCAPIFALDFDALALEALFTHLNV"
BASE COUNT         2431 a         1318 c         1293 g         2240 t
ORIGIN      
        1 cataaggcta gatcccaata atccgctgtg accagcgact tatatttttg aatttttatc
       61 cattatccat cgctacgttt tagccaccct aagactgcaa actactttaa ataaccttct
      121 catcgaacat tgtaccgtgg cattgcagaa aaacaagaat caaatttagt tataaaacga
      181 aactattcat tgtatttaag attcgtttgg tttaatgcgt ttcttttata ttcaaaaatt
      241 cccactatca aaagaaaaat gaaaaacata aaaaatataa tggaagcttg catatatacg
      301 cgagtgggag gtatccgatt ttgaactttc gctttgcctt tttggataat agaattggac
      361 tgcaaacctt gtggtatttt ttctaaaaaa ttaatcaaca taaaacctgg cattctatac
      421 gcagcaagtg ttagccaaga cgaaggatgg atggaggctt gtaacaccgc tatagtataa
      481 cctgtaactc ttgtattact ttgatcttct agagcatttt catcaacgtg atattttagt
      541 ttatcgttca atccataaaa catgacactc ataatagcaa aaaaacttaa tccaactatt
      601 atgatgtgta acgctttgtt taagagattt tttgtgttgg acttccataa tacatatcct
      661 aaaatcgcta aggggtataa ggaaaagaga aatctgtatc cgtacgcatc tgcataggat
      721 tgccatatta tgacgagcga caatggtaga ccaacataaa cgctaatcaa gattaaattc
      781 attaaaactg tttttttcat gctagctatt ttttttgcta agccatgata taaaaaagcc
      841 aatccaaaca ataaaatagg agaagtatag agtacgccaa attctgcaga aaaaagaact
      901 ttaaaaataa agggaagcct cattatcaac agtaaagtgg tggcagaaag cgatacagat
      961 cccgagtcct caatattttt taaatagata gatgagcttt ctccatacat tgtcttaaaa
     1021 gttggataaa tttcaccaaa cataaaccaa ttataagaaa taaaaggtat tactagtaaa
     1081 cctaaccaca aactaccgaa caagaaagca ttcttactag ctttgaaaaa atggggagat
     1141 tccccttctt ttagggtatg aaaagtaagt gtaattataa aaggcaacag aaatacctgt
     1201 aaatttgccg gacgaattaa caaggttaac aagatccctg cggctataaa gaaggaaaag
     1261 acgaaattta tatataaagt attgtctttg tgagaattta ttaacttaca cgtgccacaa
     1321 gtaacaagtg ctaaagaaaa aaactcataa gcatgtccca taacaggacg ataggttacg
     1381 taataaatta cacccaaccc actagctaat aaaagatgct cccatgaact gactaacgca
     1441 aacttcgcaa atgcgcgtat ataaagatat aatccagcca aaaaataaaa acttgaggca
     1501 aaaacaaaac cgaaataaga ccaggaagta ggaaataaat acgcgtttat taatacagac
     1561 gcattcgttg ctttatctat gagtgaaaaa attgtaataa aaggagcagc gagaaatccc
     1621 gggccaatag ggtgaacagg ggcacctgta ataggattga cccaatcatg aatcgaattt
     1681 ttatacttta gatcaaaatt cagtcccaaa ctaaagccat gcgataaata agacgcatcg
     1741 tctgcagtta atttttctat taagaaaata tgataaggac taaataaaag tgaaaaaaac
     1801 aatatagcaa gtgcaataaa aaaacattgt tttaatttac ttggcattga atattttgtc
     1861 atattcattt gcatcattct tctgtctttt tagcccaaac acaaatatta aaacccattt
     1921 gaaaatagcg catacgaata ttagtaaatc cagcttgttc caaggcttta tgtaaagaac
     1981 atttggaata ataacgttta tgatctagaa tttcggtcgc gtctatccat ttaagtttaa
     2041 aagccaagaa ttccaaaata ggcttcgcgt ataacgaggg cgtagttaaa attaaatatc
     2101 ctccaggttt gagtatacga aatatttcca ataaattttg ttcgggcttt tctaaatgtt
     2161 caatatttgc aagggaagtt accagatcaa aactcttatc cggcaacggt atttttttat
     2221 ttaaattccc agcaataatg cgattgccat gcgctttgag atcaacgcca taagcatcta
     2281 gatttttttt taacagccaa tgtataagtt gtcccttacc acaacccaaa tcgagaactt
     2341 tttgcgtgtt tggatgaaca cgcaaaaatt ggcgcacacg actacaacga tatctcaata
     2401 aaaactgatc aacaatcgta tttgcttgaa acacggcttc tcgttgcatc gtggactctg
     2461 tataatacgc atgtagtaaa aatattaatc tattatcctt tctaaaaata gaggtaatca
     2521 aattaatttt ttgggaattt cagacttagt ctgtactata aattatttat ttttcgattt
     2581 aaatatttat tctattcata aaaaatcagc atagtcgcgt ataccttatg aagaggtgtg
     2641 atttatgagc tatatattat ctgtcgtaat cccctgttat aacgaagcaa aaaccataga
     2701 gcatattcta aaaaaaataa gacaaagtcc cattgaagga aagttagaag tcattgtagt
     2761 aaatgatggt tcaaccgaat caatgaaaat tttattagaa accgtttgta agcctttgat
     2821 cgatgtttta atacatcatg ataaaaatca aggaaaagga gctgcattaa agacaggtat
     2881 agcacatgct aaaggagatt atgtgatcat tcaagatgcc gatcttgaat acgatcccaa
     2941 cgatttccct caactcctag cacctttttt agacaatcgt gcagatgtag tcttcggtag
     3001 tcgctttatt ggtggtgagt gtcatcgggt attgtatttt tggcattcat taggtaatcg
     3061 ctttttaaca ttactttcaa atatgtttac taatcttaat cttactgaca tggaaactgg
     3121 atataaagtc ttcaaaagaa gcattattca gtcccttccc ttacaagaaa aaagatttgg
     3181 atttgaacct gaagttacag cccgtattgc acacattcaa ggtattagac tttatgaagt
     3241 tggcatttct tactatggtc gcacttacga agaaggcaag aaaattggct ggaaagatgg
     3301 cgtgcgagcg atttattgta ttttaaaata cggattattt tgtcgcttag agcgtaagcc
     3361 attgccgcaa tatcttcgcg acaatgacac ttaatttata tacattctaa aactttatat
     3421 cctcgttctt ggcacatagc atcccgttga gccaatttta aatctgcttc caccatttct
     3481 cgaactagct ctggaaaaga aattttaggc tgccaattta atttttgctt tgctttggtg
     3541 gcatccccta ataatgtttc tacttcagta cgtcggtaat attttggatc gaccttaata
     3601 attattgttc ccgttttttt atcaattcca tgttcttcaa caccagaacc tacccatgtt
     3661 agttcgatat ttaaaaattt tgctgcgaaa tttacaaaat cacgcacact gtgttgtacc
     3721 cctgtcgcaa taacaaaatc ttcaggagtc tcttgttgta gcattagcca ttgcatttcg
     3781 acgtaatcac gcgcatgccc ccaatcacgt tttgcttcta aatttcctaa ataaaggcaa
     3841 tcttgcaagc ctaatttaat tcttgctaaa cctctagaaa tctttcttgt tacaaatgtt
     3901 tcaccacgta caggggattc atggttaaac agaatgccat tacaagcgta cagattatac
     3961 gcttctcgat aatttattgt gatccagtaa gcgtagagct tagctacagc atagggagat
     4021 cgcgggtaaa atggcgtggt ttctctctgg ggtgtttctt gaactaggcc ataaagctcg
     4081 gaactagatg cttgataaaa tcttgtttta ttttgcaaac cgataatacg aatagcttct
     4141 aaaatacgta gtgcacccaa agcgtctgta tttgccgtgt attctggctc ttcaaaggaa
     4201 tctgctacat gactttgtgc tgctaaatta tagatttcat cgggttgaac ttgctgaata
     4261 atgcgcatta aactcatagc atcggttaga tcaccatgat gtaaaatgag tttgcgattg
     4321 gtatggtcga tacgttgggt attaaaaaga gaagttcgac gctttatgcc atgtacttca
     4381 taattttttt tcaataaaaa ctctgcaaga taggaaccat cttgaccagt tatacctgta
     4441 atcaaagcta ctttagattt caagtaccta acacctttga aaattttgac ctaagtattc
     4501 taccttggta gttctgcgaa aacaaccaag ataaattgtt taaccaaata atttcaatat
     4561 ggatatttca acattagttc tgcaaaaagc aatacttcga atataataag aaaatatctc
     4621 taaaaatcgt ttgggtagtt atcataccca cttttaaaag cttcttaaat gcgaggataa
     4681 tttggctaac cataaatccc cctatgctga tttaaatatt gcagttattg gttgtggcaa
     4741 ttggggtaaa aatcatattc gagtattaga cgaactcggt gcattatacg ccgtatcaga
     4801 tgccgatgtt tggactgcag ctcactttag taatgaatat ggcgtaccga aatatacatt
     4861 agataaaatt attgccgatc aaaaaattca tggtgtggtt atcgcaacgc ctgctgccac
     4921 tcatcaagaa attgccatac gctgtttaca ggcaggcaag catgtatttg tagaaaagcc
     4981 catcgcctta acggtagcgg aagccaaaaa aattcatgag caagctaaat tgtctcgtag
     5041 aatactcatg gttggtcatt tgctgcatta tcacgcagcc tttcagaaac taaaagagct
     5101 gcacgccgag gggcatttag gccaattaca atacatttat tcgaatcgat taaatttagg
     5161 gaaatttcgc accgaagaga atatttggtg gagttttgct ccacatgatg tatctatgat
     5221 tttagcgctg gtaggagaaa tgcccaacac catcatggca atgggcgcaa attatcttat
     5281 gcacacggtt gcagatgtaa cgtgcaccca tttaaccttc cctaaaggag agcaagcgca
     5341 tatttttgtt tcgtggcttc atccttataa ggagcaaaaa ttggttgtag tcggagaaaa
     5401 ggctatggca atttttgatg atggccaacc ttggaaatcc aaattaaaat tacatccata
     5461 tccggtagag tgggtggatg gattaccaaa accttcgcaa agtgacgcca cattaataga
     5521 cattccaaaa tccgaacctt taaaagaaga agccaaacat tttttagatt gcattttaca
     5581 taataaaact cccattaccg atggtaacga aagcatacgc gtgctaaccg ttttgaatgc
     5641 tgcggaatat tccattgaga aatatcaacc ggtatatttg gccgaggccc acaacaaatt
     5701 tcagggtgaa ttagcgaata actatttttc gcatgagacc acctgcattg atccgggatc
     5761 gcatattggt agtgaaacca agatttggca ttttagccat attttacctg gcacagaaat
     5821 aggtaaaaaa tgcagcatag gacaaaattg ttgtattgga cctaatgtga gagttggtaa
     5881 taattgcaaa attcaaaata atgtaagctt atacgaaggg gttgtgatac aagacgatgt
     5941 atttgttggc ccgagtgttg tttttaccaa tgtgaatgca ccgcgggcgt ttattaaccg
     6001 taagcgcgaa ttttcaccca ccctcgtaaa aaaaggcgcc accattgggg caaatgctac
     6061 gattttatgt ggcttaacct taggcgaata ttgttttatt gccgcaggcg ctgtcgttac
     6121 caaagatgtg caaccatttg cattaatggc tggcaatcct gcacgacata tcggttgggt
     6181 gtcacaagat ggcgaacctt tagatgaaca tctttgttgt ccgcgatccg gtaggcaata
     6241 tcgactcatt aacgaaaaac tgtacgaagc cagttaaata aaaaggtcaa ggatgacaca
     6301 aacacacata gtgcaagaaa gcgcttcttt tcgagatccg gatgggtttg tgtttcaata
     6361 tcgcaatgct tggtatcgtt ttgtagatga aagttaccaa gagcattatg atcatcttat
     6421 tcattctggt ctttatgaaa gattaataca agaacgtctt ttactaccgc atcaagaaat
     6481 atctgaagaa gctttttcgt tttcgaaacc ttgttataaa attttaaaac ctagccaatt
     6541 agaattcgtt tcttatccgt atgagtggtg cttttcgcaa ttgaaagaag cagggattgc
     6601 tactctaaaa attcaaaaaa ttgcccatca atttggtatg tgtttaaaag atgcttcagc
     6661 attcaatata cagttttatc aaggaaatcc tacgcttatt gatactttat ctttccacag
     6721 gtgtacaaaa aatacaccat ggatagcaat gggtcaattt tacaggcatt ttctctggcc
     6781 gcttttagca atggctaata tagatgttac cttgaataaa caatttctct tatatccaga
     6841 cggttttaag catgaaactt taaaaccctg gtttggcata aaaacgtggt ttaaacccag
     6901 tatcttattg cacgttcatt tacatggaat ttttgcaaaa aaagcagcta taaagcaagc
     6961 aaccaaaaaa gattttcagc ttcctgagac ttacttacca atacttttag atcgtataat
     7021 ttctctgatt gaaaaattaa ccctcaaaca acaatcggta tggaaagatt attatcataa
     7081 tactaattat gaaccgcgtt cattcaaaga aaaagaattg catgttgtaa actttattca
     7141 acaaaatcga gggaaagctc tgattgattt tggcgcaaat aatggccatt ttagtatttt
     7201 ggctctaaag acttgtgccc ccatttttgc gcttgatttt gacgctttag ccttagaagc
     7261 actgtttacg catttaaatg tg
//
LOCUS       DASTIB010000074         7250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1196188, whole genome shotgun sequence.
ACCESSION   DASTIB010000074 DASTIB010000000
VERSION     DASTIB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196188"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2424
                     /locus_tag="VFP93_01250"
     CDS             133..2424
                     /locus_tag="VFP93_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HET9843267.1"
                     /translation="MPKSKILATLSNAKTRTFVLVGGGIVIVLIIYAFMQLGDEEEGL
                     GVESRTIAVPSSIKAIPGNVTPERYAQLQRLENLKLAEEAEKKRGVSIPTIIGQISDD
                     PNDPGKGFGPKGAFGPGTGTNGVGLPPGYYYGPDGRLFGPDGKPLPPGYYVGADGQIY
                     GPDGKPLPPGYYVGADGKIYGPDGRLIDAGAGATGGITSGTPNLPPGYYIGPDGRIYG
                     PDGNPLPAGYTLGQDGRIYGPDGKPIPLGYHVGQDGKIYGPDGRPLPAGYTIGNDGRI
                     YGPDGKPIPEGYSVGPNGELLPPGYYIGADGKVYGPDGKPLPEGFTIGPDGKIYGPDG
                     KPVLPPGYRLGPDGRIYGPNGQPLPEGAFVGPDGILYGKDGKPLLPPGYSIGPDGRIY
                     GPDGKPLPEGSYIGADGNIYGPDGRLIAGKTVGAGGAGMGATNAAANLLETALRAGQT
                     EEQTDEQLRDERLRQQRDELEQRRLEREKQKEADRVAKQAEQDRQLYIQELDQKTQAM
                     EQHAQSLSSAWMSITPQVLVEGTFATTELKSKNGTGNTTTIATDNDTTTVVVTKDENG
                     RLVPKVTKKVYIKAGTIMFGVLDTSINTDEPGPVLATIVHGKYKGARLIGTIEHASEF
                     AQKVIVSFQTMSLPSHDTSLAISAVAIDPDTARTALASDVDKHIIFRYGMLFASAFME
                     GYGQAISNEGTTTTTANGTTTETKPPLDGKDQVIAALGKVGEQLGQATRPYFDTPYTV
                     TVDQGLGIGLLFLSDVDVTPPEG"
     gene            2429..3187
                     /locus_tag="VFP93_01255"
     CDS             2429..3187
                     /locus_tag="VFP93_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843268.1"
                     /translation="MADENENEFEEEFDFESEEELIEPAEGEVTTFEEPRKKPPLPLL
                     VGGALAAIAALGIGAYMFMGTKEELPPPAPAPQVVEPPPPAPMVPAAPDPWDQGMLTE
                     EDSKPKVTPQNIVEKLEAQEREINQQIQALERKLNELLSKLSSLDQAIGSSNRDLAQV
                     SRKLEALNQELKMLATPAKEQEKPEAELQQSASYVNPSLTVHAIIPGRAWLKTRDGRT
                     LTVTEGDPVEGYGKVIVIDAPSGIVITSSGVVLR"
     gene            3230..3787
                     /locus_tag="VFP93_01260"
     CDS             3230..3787
                     /locus_tag="VFP93_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843269.1"
                     /translation="MIEFTSVSIDTALANLTDAVSAIKVLVSAISFIVGIFLVFKGVT
                     GYKIFATQTFASAQKGEFAGPLVFIIVGSILIYLPAATDVSLFTVFGKNASGIPDEAK
                     LIAYPGGGVEEKWTEIAVVLIKYMKLIGFIAFVRGWIILSKMGHSGSQPGSVGKGLTH
                     LIGGVLLMNIVDTVLILARTFGFTT"
     gene            3804..4415
                     /gene="icmJ"
                     /locus_tag="VFP93_01265"
     CDS             3804..4415
                     /gene="icmJ"
                     /locus_tag="VFP93_01265"
                     /inference="COORDINATES: protein motif:HMM:NF038221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmJDotN"
                     /protein_id="HET9843270.1"
                     /translation="MELLPITLSASGAWKKYHARKHNKQFILVKKRVLQRDHYTCRYC
                     GFFAKEFQEVVNIDQNYNNNKLDNLATACCFCAQCFFIDSLGLDGNSGGLIIFLPEIT
                     QADLNNFCRVLYCSMEKESAYKGKLQSVYLSLQERSKDVVNCFGPDTDDPRIFGQSLL
                     DAGVNKKQLQHEVLAHLRLLPSRKDFKPQIEYWKKTVFAKVPL"
     gene            4480..4884
                     /locus_tag="VFP93_01270"
     CDS             4480..4884
                     /locus_tag="VFP93_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmD"
                     /protein_id="HET9843271.1"
                     /translation="MSTVVQKRHSNSLLVLLAYFGVLLALFLISEFAFAGGKGGGRDL
                     GEVASGITKSMVGMQKLLSAAAYIAGLGFAIAGMMKFKAHKDQPAQVPLSQPITLLLV
                     AAGLVFLPSLISTAGETIFKSGSATDAEGGGF"
     gene            4965..>7250
                     /locus_tag="VFP93_01275"
     CDS             4965..>7250
                     /locus_tag="VFP93_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmB"
                     /protein_id="HET9843272.1"
                     /translation="MSFLDPVLDSIDSLLAWVGSSIGQDAATYCLLETADSRHTLVAK
                     DGSLISIIKLHGATQLVGPDEFERMHGGLTNAFGSALSRSGHAVQVYFYYDKENVKQE
                     ITDILSPAQSTAARLQLDLTDLFEERINHLSEYCASESCFFVLWTRPNSLTKTQLDYS
                     NKAKLAKIREQKLPPARRTQNIMAAIPELRETHDSFVRSVLNDMLDLNLYAVLLDVHT
                     AVYEIRKSVDPDITDIDWRPILPGDKLPIRNLKDEKKRDLSEVLWPPLSTQIIPRDGE
                     ITTLRTCRIGDKIYAPIYIDLFPQEIKPFFELFKRVLHTKVPWRISFLIESDGIKTLG
                     IKPLLASILSFASSQNPLISDAAELLRFYQDSTDYAIVKLRVTATTWAPFHDEKLLRM
                     RSAELLKAIQGWGHCEVAELSGDAYQGTLSSALAVTTDSVATPSVAPFNDVVYMLPFV
                     RPASPWVTGATLFRSPDGKPWPYQPGSTQQTTWIDLVYARPGSGKSVLSNSINLALCL
                     QGGIKRLPRIAIIDIGPSSAGLISLIKEALPEDKKHLAAYHRLRMTPNYSINPFDTQL
                     GCRYPTPQERSFLVNFITLLATPVGETRAYDGIADMAGMIIDELYKNLSDAHNANKYT
                     SDIEPKADAALRDIGFTPDTHTTWWEVTDALFTNGKIHEAALAQRYTSPLLADAVAIC
                     RTPVVEDLYGKIVAPTGESLIVAFGRMISSAVREYPLLSRVTAFDLGEARVVSLDLDE
                     VAKTGGEAADRQTAVMYMLARF"
BASE COUNT         2099 a         1452 c         1697 g         2002 t
ORIGIN      
        1 tatcacctgc atggatgggt acagtttcga gtccagatgg aacgcatgtt tatgaaatgg
       61 taaatacccc aactatatta gcatcaaaag atggtcaaac ccttgatata cggttaacgg
      121 gattataacc tcatgcccaa atcaaaaata ttggcaacgc tctcaaacgc aaaaaccaga
      181 acattcgtac ttgtaggtgg tggcattgtt atcgtgctca ttatctatgc atttatgcaa
      241 ttaggggatg aagaagaagg tttgggggtg gaatcgcgca cgatcgccgt gccctcttct
      301 atcaaagcta ttccaggaaa tgtgacccca gaacgctacg cgcaattgca aagattagaa
      361 aatttaaaat tggcagaaga agccgaaaaa aaacgtggtg tttcaattcc tactattatt
      421 ggccaaattt cggatgaccc taacgatcct ggcaagggtt ttgggcctaa gggagcattt
      481 ggccccggga ccggaactaa cggtgtcggt ttacctcctg gttattatta cgggccagat
      541 ggtcgattgt ttggcccaga tggcaagcct ttgcctcccg gttattacgt aggtgcggat
      601 ggtcaaatct acggaccgga tggtaagcct ttgcctccgg gttattacgt tggcgctgat
      661 ggcaaaattt acggtccaga tggcagactt attgatgctg gtgcgggtgc cactggtggt
      721 attacctcgg gcacacctaa cctcccacct ggctattaca ttggaccaga tggaagaatt
      781 tacggaccgg acgggaatcc tttgcctgca ggatatacac ttggccagga cgggagaata
      841 tatggtccag atggtaaacc cattcctttg ggttatcacg ttgggcaaga tggtaaaatt
      901 tatggtcccg acggtcggcc tttacccgca ggttatacca taggaaacga tgggagaatt
      961 tatggccctg atggtaaacc tattccagaa ggttattctg ttggacctaa tggggagctt
     1021 ttgcctcccg gctattacat tggagcggat ggcaaagtgt atggacctga tggtaaacct
     1081 ttaccagaag gttttactat tgggcccgat ggtaaaattt atggaccaga cggcaaacca
     1141 gttttgccac ctggataccg cttaggtcct gatggcagaa tttatggacc caatggccaa
     1201 cctcttccgg aaggagcatt tgttgggcct gacggtattc tttatggcaa agatggcaaa
     1261 ccactgttac ctccgggtta ttcgattggt ccggatggaa gaatttatgg tccagatggt
     1321 aaacctttac ccgaaggatc gtatattggt gcagatggca acatatatgg tccggatggt
     1381 cgtctgatag cgggcaaaac cgttggtgct ggcggagcag gtatgggtgc aaccaatgcc
     1441 gcagctaatc ttttagaaac ggcgcttaga gctggacaaa cagaagaaca aaccgatgag
     1501 caattacgcg atgagcgttt gcgccaacaa cgtgatgaat tagaacaacg gcgtttagag
     1561 agagagaagc aaaaagaagc ggatcgtgtg gcaaaacaag ccgagcaaga tcgacagctt
     1621 tatatacaag aactcgatca aaaaacgcaa gccatggaac aacatgcaca atctttgtcg
     1681 agcgcttgga tgtccattac cccacaagtt ttagttgagg gtacttttgc aactacggaa
     1741 ctgaaaagca aaaatggaac gggcaatacc actacgattg caaccgataa tgacactact
     1801 acggttgttg tgaccaaaga tgaaaatggc agattagttc ctaaggtaac gaaaaaagtt
     1861 tatatcaaag cgggtacgat tatgtttggt gttttggata catctattaa taccgatgaa
     1921 ccgggacctg tgcttgcgac tattgtgcac ggtaagtata aaggtgctcg cttgataggc
     1981 accatcgaac atgccagtga atttgcgcaa aaagttattg tttcgttcca aaccatgagt
     2041 ttacccagtc atgacacttc tttagccatt agcgcagtag caattgatcc tgatactgcg
     2101 cgtaccgctt tagcatcgga tgtggataaa catattattt ttcgttacgg aatgttattt
     2161 gcttctgctt ttatggaagg atacggtcaa gcgatatcga acgaaggaac aacaacgact
     2221 acagcaaatg gaacaacaac agaaacgaag ccgccacttg atggtaaaga ccaggttatc
     2281 gcagctttag gtaaagttgg tgaacagttg ggtcaagcta cgagacccta ttttgacacc
     2341 ccctataccg ttacagtaga tcagggttta ggtattggct tattattttt gagtgatgtt
     2401 gacgtcacgc caccagaggg ttgacaaaat ggcagatgaa aatgaaaacg aatttgaaga
     2461 agagtttgac ttcgaatctg aagaagaact tatcgagccc gcagaaggcg aagtcaccac
     2521 gtttgaagag ccgagaaaaa aaccgccctt acccttgttg gtaggaggtg cgttagcagc
     2581 gattgcagct ttaggaattg gagcttatat gtttatggga acaaaggaag agcttcctcc
     2641 tcctgctcca gcgcctcaag ttgtggaacc tcctccgcct gcgcctatgg ttcctgcagc
     2701 gcctgaccct tgggatcaag gcatgttaac ggaggaagat agtaagccca aagttacacc
     2761 gcagaatatt gtagaaaaat tggaagcaca agaacgtgag attaatcagc aaatacaggc
     2821 attagagaga aaattgaatg aacttctttc taagttaagt agtcttgatc aagcaatcgg
     2881 atcttccaat cgagatttag cgcaagtttc acggaaactg gaagctttaa atcaagaatt
     2941 aaaaatgttg gccacaccag ccaaagaaca ggaaaagcca gaagcggagt tacaacagtc
     3001 agcatcttac gtcaatccaa gcttgaccgt acacgcaata attccgggaa gagcgtggct
     3061 caaaacacga gatgggcgta ctttaaccgt aacggaaggc gaccctgtag aaggatatgg
     3121 taaagtaata gtaatagatg ctccatcagg gatagtgatt acaagctcag gtgtagtgtt
     3181 acgatagtaa aggaagtagc gggagtcgca ccatggtttg aggtagtgca tgattgaatt
     3241 cacaagcgtg tccattgata ccgctttagc gaatcttacc gatgcggtca gcgcaatcaa
     3301 agtgcttgtg tctgcaattt cttttattgt cggcatattt ctcgttttta aaggcgtcac
     3361 gggttataaa atattcgcga cccaaacgtt tgcttctgcg caaaaaggtg aatttgcagg
     3421 tccacttgtt tttattattg tcggaagcat tttaatctat ttgcccgcag ccacggacgt
     3481 atcccttttc actgttttcg gaaaaaacgc gtccgggatc ccagatgaag caaagttaat
     3541 cgcatatccg ggtggcggcg tggaggaaaa atggactgag atagccgtag ttctgataaa
     3601 atatatgaaa ctcattgggt ttatcgcttt tgtgcgcggg tggataattt tatcgaaaat
     3661 gggtcactca ggcagtcaac ctggcagcgt aggaaaaggt ttaacgcatc ttattggcgg
     3721 cgttttatta atgaatattg tggataccgt gttaattttg gcacgcacct ttggatttac
     3781 aacataatta aggggagttt aggatggagt tattacccat caccctatcc gccagtggag
     3841 catggaagaa atatcacgca agaaagcata ataaacaatt tattctcgta aaaaaaagag
     3901 ttttacaaag agatcattat acgtgccgat attgtggctt ttttgccaag gaatttcagg
     3961 aagttgtaaa tatcgaccag aactataaca acaacaaact ggataatttg gcgactgcgt
     4021 gctgtttttg tgcacagtgt ttttttatag actctttagg attagatgga aattccggtg
     4081 gtttaattat ttttttaccg gagataacac aggcagatct taataatttt tgtagagttt
     4141 tgtactgttc catggaaaaa gaatccgctt ataaagggaa attacagtcg gtgtacttaa
     4201 gtttacagga gcgtagcaag gatgtagtta attgttttgg acccgacacg gatgatccac
     4261 gcatttttgg gcaaagtttg ctggatgcgg gcgtaaacaa aaaacaattg caacatgaag
     4321 tgcttgccca tttgcggtta ctgccttcac ggaaagattt taaaccgcag atagaatatt
     4381 ggaaaaaaac ggtatttgca aaagtaccac tatagggatg attttattat taacagcatg
     4441 gtataaacta gctatactgt gtatttggag gagataatta tgagtactgt tgttcaaaag
     4501 cggcatagta attcgcttct ggtgttactt gcttattttg gcgtcttatt agctttattt
     4561 ctgatatcag aatttgcctt tgctggcggt aaaggtggcg gtcgcgactt aggtgaggta
     4621 gcgagtggta tcacaaaaag catggtaggg atgcaaaaac ttctatctgc agcggcttat
     4681 attgcagggt tagggtttgc gattgccggg atgatgaaat ttaaagcgca taaagaccaa
     4741 cctgcacaag ttcccttaag tcaacctatc acgctattat tggtggcagc aggattagta
     4801 ttcctaccaa gcctcattag tactgctggg gaaacgatct ttaaaagtgg ctcagccaca
     4861 gatgctgagg gtggcggatt ctaaaatccg ataaccttaa tagtaccaag catatgtctt
     4921 aaagaattgg atgaggcata tgcttataac taacgagact atgcatgtct tttttagatc
     4981 ctgttttaga cagtatcgat agtttacttg cttgggttgg atcctcaatc gggcaagatg
     5041 ctgcaaccta ctgcctctta gaaacggcag atagccgaca tacgttagta gctaaagatg
     5101 gctctcttat ttctattatt aaattgcatg gcgcaacaca attagtaggt ccagacgagt
     5161 ttgagcgcat gcatggtggt cttaccaatg cgtttggctc agccctatcc cgctctggcc
     5221 atgcggtgca ggtgtatttt tactacgaca aagaaaacgt taaacaagaa attaccgata
     5281 tattgagccc ggcacaaagc acagctgccc gactgcaatt ggatttaacc gatctctttg
     5341 aagaacgcat caaccattta agtgaatatt gcgcttctga atcttgtttt tttgtgctgt
     5401 ggaccagacc aaattcttta acaaaaacac agctcgatta ttctaataaa gctaagcttg
     5461 ccaaaattcg tgagcaaaaa ttacccccag cgcgacgcac ccaaaatatc atggctgcaa
     5521 ttcctgagtt acgggaaaca cacgattctt ttgtgcgcag cgttctaaac gatatgctag
     5581 atctaaatct gtatgccgtt ttactcgatg tgcatacggc cgtatatgaa atacggaaaa
     5641 gtgttgatcc agacatcacc gatatcgatt ggcgacctat tttaccgggt gataaattac
     5701 ccattcgaaa cttaaaagat gaaaaaaaac gtgatctatc tgaagtactc tggcctcctt
     5761 taagtacgca aattattccg cgcgatggcg aaatcacaac ccttcgcact tgtagaattg
     5821 gggataaaat atatgcccca atctatattg atttatttcc tcaagaaatt aagccttttt
     5881 ttgaattatt taaacgggta ttgcacacaa aagttccctg gcgtatttct tttctcatag
     5941 aatcggatgg tattaaaacg ttgggaataa aacccctgct tgcttctatt ttaagctttg
     6001 cctctagtca aaatcctctt atctcggatg cagcagaatt attgcgtttt taccaggaca
     6061 gcaccgacta cgcgatcgtt aagcttcgtg ttaccgcaac aacctgggca ccatttcatg
     6121 atgaaaaatt attgcgaatg cgttctgcag aattattgaa agcaattcaa ggctggggac
     6181 attgtgaggt ggcagaacta tctggcgatg cataccaagg tacattatcc agtgcgcttg
     6241 cggtgaccac cgatagcgtt gcgactccat cggtcgcccc atttaatgat gtggtttata
     6301 tgttgccatt tgtgcgacct gcgtcacctt gggtaacggg cgctactttg tttcgttcac
     6361 cagatggtaa accctggcct tatcaaccag gatctactca acaaacaact tggattgact
     6421 tggtttatgc gcgtcctgga tcgggtaaat ctgttttatc gaattctata aaccttgcgt
     6481 tgtgcttgca aggcggtata aaacgtttgc ctcgtattgc aattattgat atcggaccat
     6541 ccagtgctgg acttatttcc ctgataaaag aagcgttacc agaagacaaa aaacacttag
     6601 cggcctatca tcgcttaaga atgacgccaa attattctat taatcctttt gacacacaat
     6661 taggttgtcg ttaccctaca ccgcaagaaa gaagtttttt agtcaatttt attacgctgc
     6721 tagccacacc ggtgggtgag actcgagctt atgacggtat agcagatatg gcagggatga
     6781 ttatcgatga actttataaa aatctatcag atgcacacaa tgctaacaaa tacacgagcg
     6841 atattgagcc taaagcagat gctgcattaa gagatatagg ctttactccc gatacccata
     6901 ctacctggtg ggaagtaaca gatgcattat ttaccaatgg aaaaattcat gaagcggcat
     6961 tagcacaacg ctatacctct cccctacttg ccgatgcagt tgccatttgt cgtacccctg
     7021 tcgtggaaga tttatacggt aaaattgtcg ctcctacggg tgaaagtctt atcgttgctt
     7081 ttggacggat gatatcgagt gctgttcgag aatatccttt attatcgcgt gtaaccgctt
     7141 ttgatttggg cgaagcacgg gttgtttctc tcgatttaga tgaagttgcc aaaacgggtg
     7201 gtgaagctgc agatagacaa acagcggtaa tgtatatgct ggcacgtttt
//
LOCUS       DASTIB010000075         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_333621, whole genome shotgun sequence.
ACCESSION   DASTIB010000075 DASTIB010000000
VERSION     DASTIB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333621"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..524)
                     /gene="tolR"
                     /locus_tag="VFP93_01280"
     CDS             complement(57..524)
                     /gene="tolR"
                     /locus_tag="VFP93_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HET9843273.1"
                     /translation="MRNRRRRENKSLVADINVVPYIDVMLVLLVIFMVTAPLLQQGVA
                     VDLPQASANPIEPSEKEPLVVSVDAQGAYFLNIADNPNKPIEPQALMVRVAAELDRDK
                     NRKILVKGDRNVDYGQVMGAMVLLQKAGAPGVGLVTQDNDSDTAMAKSNKHSS"
     gene            complement(524..1234)
                     /gene="tolQ"
                     /locus_tag="VFP93_01285"
     CDS             complement(524..1234)
                     /gene="tolQ"
                     /locus_tag="VFP93_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HET9843274.1"
                     /translation="MQADISLFELFWHASLLVQGVMLLLLIISMTSWTLIFQRGMVIK
                     KASKIFHEFEHKFHLGHDLNRLYDYIASKRERSEGLENIFKAGFREFLRLNKKETMSP
                     KVVMENTERAMRVALAQEEDQLEKHLSFLASVGSISVYVGLFGTVWGIMTAFRALGSM
                     QQATLAMVAPGISEALVATALGLFAAIPAVFAYNRYTQHVQSLLRKYDNVLDEFTGLL
                     QRQIYLKHHADSEREERD"
     gene            complement(1209..1637)
                     /locus_tag="VFP93_01290"
     CDS             complement(1209..1637)
                     /locus_tag="VFP93_01290"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HET9843275.1"
                     /translation="MTQHSSFFKIYYEDIDIGGIVYHANYLKFMERGRTDFLHRCGFD
                     LHTLLIQEGVQFAVKDLQITFIKSAKLGDEIIVLTELTELSGAKLIFNQQIATKKADD
                     LTTLCTGKIVVVCLDRYLKLIRLPSRLKEELYRASRHFVI"
     gene            complement(1678..2658)
                     /gene="ruvB"
                     /locus_tag="VFP93_01295"
     CDS             complement(1678..2658)
                     /gene="ruvB"
                     /locus_tag="VFP93_01295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET9843276.1"
                     /translation="MISPQLGAQENQFDRAMRPKYLRDYVGQAKTCEQLTLFIQAARN
                     RLEALDHILIFGPPGLGKTTLALIIANEMEVGFKHTSGPVLERPADLAALLTQLEPRD
                     VLFIDEIHRLSPVIEEILYPAMEDNQIDIMIGEGPAARSIKLDLPPFTLIGATTRAGM
                     LTSPLRDRFGIISRLDFYSVVELTKIVIRSAMLLKVPIPEGAQEIAKRSRGTPRIANR
                     LLRRVRDVAEVEGSGTITQDIAEKALALLEVDANGFDVLDRKLLLTAIEKFSGGPVGL
                     DTLSAAIGETRETIEDIIEPFLLQQGYILRTPRGRVVTPKTYVHFGLKSP"
     gene            complement(2681..3265)
                     /gene="ruvA"
                     /locus_tag="VFP93_01300"
     CDS             complement(2681..3265)
                     /gene="ruvA"
                     /locus_tag="VFP93_01300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020912459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET9843277.1"
                     /translation="MIGRIRGKLCEKRPPHVLIDVHGVGYSILAPLSTFLQLPDLEQE
                     VILYTEFIVREDAQTLYGFIKEEDKALFQQIIKVNGVGPKVALGILSGMEPHFFVECI
                     QQENLNALTKLPGIGKKTAERLVIELRDKLKSMQREIPMLEKNSTTTQSKQEAIAALE
                     ALGFKSREAEHAISKVYHETKTAEELIRLALQGS"
BASE COUNT          963 a          725 c          616 g         1012 t
ORIGIN      
        1 caaaaccacg tgtatggcaa tagctaaaat aaaagaccac gaatacgaat gctttttcat
       61 gaactgtgct tattactctt ggccattgcg gtatcggagt cattatcttg tgtgaccaac
      121 cctacgccag gtgcacccgc cttttgcagc aataccatcg cccccataac ttgaccatag
      181 tccacgttgc gatcgccttt gactaaaatt tttcgatttt tgtcacgatc caattcagct
      241 gccacgcgta ccatcagcgc ttgcggttcg atgggtttat tgggattatc cgcaatattt
      301 aaaaaatacg caccctgtgc atccacgctc accactaagg gttctttttc tgagggttca
      361 ataggatttg cactcgcttg gggtaaatcc actgctacac cttgttgcaa taaaggagca
      421 gttaccataa aaataacaag tagcactaac attacgtcga tataaggcac cacattaata
      481 tctgctacta aagatttatt ttcgcgtctg cggcgattgc gcattaatct ctctcctcgc
      541 gttcgctatc tgcatgatgc tttaaataaa tttgacgctg gagtaaaccc gtaaattcgt
      601 ctaatacgtt atcgtattta cgcaatagag attgcacatg ttgggtgtaa cggttatacg
      661 caaatactgc aggaatagct gcaaataacc ctaatgcggt ggcaaccaac gcttcagaaa
      721 tcccaggggc taccatagcg agtgttgctt gctgcataga gcctaaagcg cgaaaagcag
      781 tcattattcc ccaaacggta ccgaataagc ccacatatac gctaatagaa cccacgctgg
      841 ctaaaaagga taaatgcttt tctaattgat cttcttcttg cgctaacgcc acgcgcatag
      901 cccgttcggt attctccatg actaccttag gactcatcgt ttctttttta ttgagacgta
      961 aaaattctct aaaacctgct ttaaaaatat tttccaaacc ttcggaacgc tcacgcttag
     1021 atgcaatgta atcgtatagt cgatttagat cgtggccgag atgaaacttg tgttcaaatt
     1081 catgaaaaat tttagatgct ttttttatga ccatgccgcg ttgaaaaatg agcgtccacg
     1141 aggtcataga aataattaac agtaacagca tcacgccttg caccaaaagg cttgcatgcc
     1201 aaaagagttc aaataacgaa atgtctgctt gcacggtaga gttcctcttt taatcggctc
     1261 ggtaagcgaa taagctttaa ataacgatct aaacagacta ccacaatttt tcctgtacac
     1321 aatgtagtaa gatcatcagc ttttttagtt gcaatttgct ggttaaaaat taactttgct
     1381 ccacttaatt ctgtcaactc agtaagtact atgatttcat cacctaattt ggcagattta
     1441 ataaatgtaa tttgcaaatc cttgaccgca aattgtacac cttcttggat caataacgta
     1501 tgtaaatcaa agccacatcg gtgcaaaaaa tcagttcggc cgcgttccat gaattttaaa
     1561 tagttagcgt gataaacaat accaccaatg tcaatatctt cataataaat tttaaaaaaa
     1621 gaagaatgct gtgtcataga tctatcacaa tttgtgctga tttaattaaa aaatgtatta
     1681 gggtgatttt aaaccaaaat gcacatacgt cttgggagtt accacacgtc cgcgtggagt
     1741 ccttaaaata tagccttgtt gcaaaagaaa aggctcaata atatcttcaa tagtttcgcg
     1801 cgtttcacct atggcagccg atagcgtatc taagccaacc ggtccaccgg aaaatttttc
     1861 aatggcagtc aaaagtagtt tcctatctaa cacatcgaaa ccatttgcat ctacttccag
     1921 aagtgccaaa gctttttcag ctatgtcttg cgtgatagta ccactcccct caacttccgc
     1981 aacatctcga actctgcgta atagtcgatt ggcgatacgc ggcgtcccac gcgaacgttt
     2041 tgcaatctct tgcgcacctt ctggaatagg cactttcaac agcatagcag aacgaatcac
     2101 tattttggtt agctctacaa cagaataaaa atccaaacgg gaaattatcc cgaatcgatc
     2161 gcgcagagga gaagttagca ttccagctct tgttgtggcg ccaattaacg taaagggtgg
     2221 taaatctaac ttaattgagc gggcagcagg tccttctcca atcattatat cgatttggtt
     2281 gtcttccatg gctggatata aaatttcttc gataacagga cttagtcgat gaatttcatc
     2341 aataaataaa acatcgcgtg gttcaagctg agtaagaaga gcagctaaat ctgcaggacg
     2401 ttctaaaaca ggacctgatg tatgtttgaa tcccacttcc atttcgttag caatgattaa
     2461 tgctaacgtg gtttttccca atccaggcgg tccaaaaata agaatatgat ctaacgcctc
     2521 taagcgattg cgagctgctt ggatgaataa ggttaattgt tcgcatgttt tggcttggcc
     2581 tacgtaatcc cgcaaatatt tagggcgcat tgcgcgatca aattggtttt cttgtgcacc
     2641 taattgaggg cttatcatac gagcaatggg tgaatccact ttatgatcct tgcaacgcta
     2701 gccgaataag ttcttcagcg gtttttgttt catggtaaac ctttgaaatt gcatgttcgg
     2761 cttcgcgtga tttaaaacca agtgcttcga gtgctgcaat tgcttcttgt ttactctgtg
     2821 tggttgtact atttttttct agcataggaa tttccctttg catagatttt agcttatctc
     2881 gcaactcaat cactaaacgc tccgctgttt ttttgcctat gcctggtaat tttgttaaag
     2941 catttagatt ttcttgttga atgcactcga caaaaaaatg gggttccatt ccagacaata
     3001 tacctaatgc tacttttggt cctaccccat ttactttaat gatttgctga aataatgcct
     3061 tatcttcttc tttaataaag ccatagagtg tttgcgcatc ttcacgcaca ataaattctg
     3121 tatagagtat tacttcttgt tccagatccg gtaattgcag aaaagtggat aatggcgcca
     3181 atatactata acccacacca tgcacatcga ttaacacgtg aggaggtctt ttctcacaca
     3241 attttccgcg tattcttcca atcatatttt aaccattgta aggatcgttt agatttgcct
     3301 ctgcgtgcaa agcttt
//
LOCUS       DASTIB010000076         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_947139, whole genome shotgun sequence.
ACCESSION   DASTIB010000076 DASTIB010000000
VERSION     DASTIB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947139"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="VFP93_01305"
     CDS             <1..1694
                     /locus_tag="VFP93_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9843278.1"
                     /translation="LVLMALITLSVGLILTSGFAAFMVGHLNMISIAFALLFIGLSND
                     YSIHILLRLRESMRKYTTQTAIFSSLSAVAPSLLLCAVSTTIGFFAFLPTAFHGVSEL
                     GLIAGTGILISFVLHFTFLPALLTLFPPKNTYTTRDSTWNKITELPFVYRKTILFTFF
                     ALCIGSLFLIKNLQFNADPMQLRDPTQPSVITFLELDKKLDKSLLSITYLANDFQELG
                     EIKENILKLPSVKEVMTIHDFIPDNQKKKIAEIAAIRELFHSIKITKTSPETLVSRIA
                     SLKALITLLSPYAHYSIVKDFRVNAQDFIERTGKLPLFEQVRTWDNLSYKLLRGLEYH
                     RSQMQQGLLGEPFIVEDLPAELLSRWKQNATYRIEIFPKESMENIKNQQTFVHEVLSV
                     VPHATGLPVIHEKAGETVVTAFVEAFLTAFIMILLLLILLMQNLKQIILTLIPLLAAC
                     LITIAVAVVFNVDFNFANVIALPLLLGIGIDNGIHLVHRYYSDWEHKTSLLASSTAKA
                     VIVSTLTTIFSFMNLAFSPHQGMASLGIILTVGMIAASLSTLLFLPALLKTWNGK"
     gene            1764..2867
                     /gene="hpnH"
                     /locus_tag="VFP93_01310"
     CDS             1764..2867
                     /gene="hpnH"
                     /locus_tag="VFP93_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET9843279.1"
                     /translation="MAIPFRQVFKIGFYLLRKRLTGQTRYPLVLMLEPLFQCNLACAG
                     CGKIDYPTEILQKRLTIEQAIQAVEECQAPVVSIPGGEPLIHKEMPTMVQALVERKKF
                     VYLCTNALLLKKRIGEFKPSPYLCFSIHLDGNRAKHDKSVCREGVYDKVIEAIQDALA
                     KKFRVTINCTLFNNEDPETIADFFEDVTHLGIEGITLSPGYSYAHAPRQDVFLGKRQS
                     KELFRQIFKIKQERKAKWPINHSSLFIDFLCGNQTYQCTPWSNPTFNVFGWQKPCYLL
                     VDEGHTDSFQSLMQDTVWTRYGSGKNPKCQNCMAHCGYEGSAIIDTVQNPLKAFKVFL
                     KGPKTEGPMAPELPILYNQHLTVKTIRVNHEQK"
     gene            2854..>3672
                     /locus_tag="VFP93_01315"
     CDS             2854..>3672
                     /locus_tag="VFP93_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9843280.1"
                     /translation="MSKNRLLITGGAGFIGSTIAKAALKQDFDVTILDLPNMCENVTG
                     VHAIGCDILDYPNLEKSLKHMDYVIHAAANPSLWSTNFSHLYQTNYIGTLNVIKACSI
                     HRVKRLVHISSDCTLLSKKNPIVNEISKTSFQDMPGIYAQSKWKAEQAILYKSSIPTL
                     VINPGIPLGATSQNTPFIALIQSFLTNKYYGYIHGNLGVIDVEDIALCALQALTLGKV
                     GHRYLAVSEVWSIRTLLEKLQLLSHTQSHLFKIPRSLAFLIASFAEIYALWSKKA"
BASE COUNT         1137 a          662 c          680 g         1193 t
ORIGIN      
        1 gactcgttct catggcttta atcaccttaa gtgtgggttt aattttaact tccgggtttg
       61 ctgcctttat ggttggccat ttaaatatga tttctattgc ttttgcttta ttatttattg
      121 gcttaagcaa tgattattca atccatatcc tcttgcgatt gcgagaaagt atgcgcaaat
      181 atacgacgca aacggcaata ttttcgtcgt tatctgcggt ggctccctcc cttttacttt
      241 gcgctgtatc aactaccata ggattttttg catttttgcc tacggcattt catggtgtgt
      301 cggaactagg tcttattgcg ggcacaggaa ttttaataag ttttgtgtta cattttacat
      361 ttctccctgc gcttttaaca ctatttcctc ccaaaaatac ttatacaact cgtgattcca
      421 cttggaacaa aattacggaa cttccatttg tgtatagaaa aactattttg ttcacctttt
      481 ttgcactatg tataggatcg ttatttttaa tcaaaaattt acaatttaat gccgatccca
      541 tgcaattgcg tgatccaaca cagccttcgg tgataacttt tttggaatta gacaaaaagt
      601 tagataaatc tttattatcg attacctatt tagcaaacga tttccaagaa ttaggggaga
      661 ttaaagagaa tattttaaaa cttcctagcg taaaagaagt tatgacgatt catgatttta
      721 tacccgataa tcaaaagaaa aaaatagcgg aaattgcggc aatcagagaa ctatttcaca
      781 gtataaaaat aacaaaaaca tcgccagaga ccttagtgtc aagaatagca tctttaaaag
      841 ctttgattac actattaagt ccttatgccc attattctat agtaaaagat ttcagagtta
      901 atgcgcaaga ctttatcgag cgtactggaa aattacctct tttcgaacaa gttcgcactt
      961 gggataattt atcgtataaa ttgttgcgtg gattggaata tcatcgtagt cagatgcagc
     1021 aaggattatt aggagagcca tttatagtag aggatctacc tgcagaatta ttaagtcgtt
     1081 ggaaacaaaa cgccacctat cggattgaga tttttcctaa agaaagcatg gaaaacatta
     1141 agaatcaaca aacatttgtc catgaagttt tatcggttgt accgcatgct actggcttac
     1201 ccgttattca tgaaaaagca ggggaaacgg tggtaaccgc ctttgtagaa gcttttttaa
     1261 ccgcgtttat catgatctta ttactactga ttttattaat gcaaaatcta aaacaaataa
     1321 ttttaacgtt gattccttta cttgctgctt gtcttattac tattgcggta gctgttgtct
     1381 ttaacgtcga ttttaacttt gcgaatgtta ttgcgctgcc acttttgctc ggtattggca
     1441 ttgataatgg catacatctt gtgcaccgct actatagcga ttgggaacac aaaactagtt
     1501 tactggccag tagcacagca aaagcggtaa ttgtaagcac cttaacaact atttttagtt
     1561 ttatgaatct ggctttttct ccgcaccaag gcatggcttc cttaggaata attttaacgg
     1621 taggaatgat tgcagcgtct cttagcacat tgctcttttt acctgcgctt ttaaaaactt
     1681 ggaatggcaa atgataggct ttcaactata tttgttataa agcattctaa aaaaatggac
     1741 cgagtttaac tacaaggaaa agtatggcaa ttccttttcg ccaagttttt aaaattggat
     1801 tttatttact acgcaaacgt ctaaccggac aaacccgcta tcctcttgta ttaatgctag
     1861 agcccctatt tcaatgtaat ttggcttgtg caggctgcgg aaaaattgat tatccaacag
     1921 aaattttaca aaaaagactt accattgagc aggctattca agcggtagag gaatgccaag
     1981 ctcccgttgt ttcgattccc ggtggtgaac cactcattca caaagaaatg cccactatgg
     2041 tgcaagcact cgtggaaaga aaaaaatttg tgtatctttg caccaatgcg ttgctattaa
     2101 aaaaacgtat tggggaattt aagccctctc cctatttatg cttttcgatt catttagatg
     2161 gtaatcgggc aaaacacgat aaaagtgtgt gtcgtgaggg ggtgtacgat aaagtaattg
     2221 aggcaatcca agatgctctg gcaaagaaat ttcgggttac aataaattgt accttattta
     2281 acaatgaaga tcctgaaact attgccgatt tttttgaaga tgtaacacac ttaggaattg
     2341 aaggtattac gctatcaccc ggttatagtt atgcgcatgc tccaaggcaa gatgtatttt
     2401 taggcaaaag acaaagcaag gaattgttta gacaaatatt taagattaaa caagaacgca
     2461 aagccaagtg gcccattaat cactctagct tatttattga ttttttatgt ggaaatcaaa
     2521 cgtatcaatg cactccatgg agtaatccaa cctttaatgt ttttggttgg caaaaacctt
     2581 gttatttact agtagatgaa ggacatacgg attcttttca atcgcttatg caagatacgg
     2641 tttggacaag gtatggaagt gggaaaaatc caaaatgtca aaattgcatg gcgcactgcg
     2701 gttatgaggg aagtgctatt attgacacag ttcaaaatcc tttaaaagcc tttaaagttt
     2761 ttcttaaagg tccaaaaaca gaagggccga tggccccgga attacccatt ttatacaatc
     2821 aacatcttac tgtaaaaacc attcgagtga accatgagca aaaatagact tttaattacc
     2881 ggtggcgcag gatttattgg ctctacgata gcaaaagctg ctctcaaaca ggattttgac
     2941 gtaactattt tagatctacc caatatgtgt gaaaatgtta cgggtgttca tgccattggc
     3001 tgtgatattt tagattatcc taaccttgag aaatcgttaa aacatatgga ttatgtaatt
     3061 catgctgcag ccaatccaag tctttggagt actaattttt cgcatttata ccaaactaac
     3121 tatataggta ctttaaatgt aatcaaagcc tgtagcattc atcgtgtgaa acgtttagtt
     3181 catatctcat cggattgtac cttgctgtcg aaaaaaaatc ctatcgtaaa tgaaatttca
     3241 aaaacttctt ttcaggatat gcccggtatt tatgcgcaat caaaatggaa agcagaacaa
     3301 gcgatacttt ataagtcttc gattccgaca cttgtgatta atccaggtat tccgttggga
     3361 gcaacctcgc aaaacacgcc ttttattgcc ctaatacaat cttttctgac aaataaatat
     3421 tatggttata ttcatggtaa cttaggggtt attgatgtag aggatatagc actttgcgct
     3481 ttacaagcct taacattggg taaagttggc catcgttatt tagcggtatc cgaggtttgg
     3541 tctatacgta cgttgttaga gaaattgcaa ctattaagcc atacgcaatc gcatttattt
     3601 aaaattccac gatcgttggc atttttaata gcgagttttg ccgaaattta tgctttatgg
     3661 tccaaaaaag ca
//
LOCUS       DASTIB010000077         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1106590, whole genome shotgun sequence.
ACCESSION   DASTIB010000077 DASTIB010000000
VERSION     DASTIB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106590"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFP93_01320"
     CDS             complement(<1..488)
                     /locus_tag="VFP93_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9843281.1"
                     /translation="MQKSNSKWLWPFAMFFVATFFYLYEFLLRVIPSVMLPQLFLEYQ
                     IGAEEYGVLASIYYASYAPLQLPVGAWIDRYGTRRLLTFAILLCALSTLLSAYTTNFE
                     IALLARLLIGAGSAFGFISCIKIVAIWFPPSWFPILTGFTLTIGTLGAVASAPISFAM
                     NF"
     gene            626..1921
                     /gene="hmgA"
                     /locus_tag="VFP93_01325"
     CDS             626..1921
                     /gene="hmgA"
                     /locus_tag="VFP93_01325"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET9843282.1"
                     /translation="MDTMDLAYLCGFGSYWESEALPNALPKGQNSPKKNAYGLYTEQL
                     SGSAFTRLRHENLRTWLYRILPSVLQEDFTAYKGIESFHHNASYAPPPNQMRWDPLPI
                     PDKPLDFIHSLQHVLYNGDPCGHLGGAAIYVFCATTSMQDTYFYNADGELLIVPYLGA
                     MRIRTELGILEIVPGEILCVPRGVKFCAEVLGSVAAGYVCENWGAPFSLPYLGPIGSN
                     GLANPRDFKIPMANYEDKSGECLLICKYLNNFWQASLSHSPLNVVAWHGNYSPYKYDL
                     RLFNTINTVSFDHPDPSIFTVLTSPTSTPGLANVDFVIFPERWMVAEHTFRPPYYHRN
                     IMSEYMGLIYGVYDAKSSDFKPGGGSLHNCMSAHGPDKETFLKAQQDSESPYKITDTL
                     ACMFESSKPWIPTPWANHTPLRQKNYVHCWQNLPKLFNK"
     gene            complement(1969..2295)
                     /locus_tag="VFP93_01330"
     CDS             complement(1969..2295)
                     /locus_tag="VFP93_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9843283.1"
                     /translation="MLTKPIKDRQTRVEILQHISERTNLKRVDVEAVFDAMAGLIKAH
                     LSKRGSGEILLPKLGIRIRKVRRNRTKARTMVSPLTGQEVTIPSKPARDDIKLIALKP
                     LKEALT"
     gene            complement(2463..>2995)
                     /locus_tag="VFP93_01335"
     CDS             complement(2463..>2995)
                     /locus_tag="VFP93_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET9843284.1"
                     /translation="IGNPPTLQFMEWGFIPSWTNKNVQTPSRYINARCETLHEKVTFK
                     DSFRKRRCLIPASGYYEWKLIKNKKQPFYLHAPEFPILAFAVIWDSWKNSNFEIKETV
                     AIVTKSADQFMDLQERSPIVIPSDFYAQWLDPLTPLDSLLSLLLKTQPHFTSYPVSTR
                     VNNPKFEDSSCLQSLV"
BASE COUNT          899 a          626 c          601 g          869 t
ORIGIN      
        1 ataaaattca ttgcaaacga aatgggtgca cttgctaccg cccccaaagt tccaatggta
       61 agtgtaaaac ccgttaagat gggaaaccaa gaaggtggga accaaatggc gacgattttt
      121 atacaagata taaaaccaaa agctgatcct gcacctatta ataaccgcgc taacaatgct
      181 atttcaaaat tggtggtgta tgcactcaat aaagtgctta atgcacataa gagaatagca
      241 aaggttagca aacgtcgcgt gccgtaacga tcaatccacg ctcccaccgg cagttgaagt
      301 ggagcataag aagcataata aatggatgct aataccccat attcttcggc accaatttgg
      361 tattccaaaa ataattgcgg tagcataaca ctagggatta cccgcaataa aaactcgtat
      421 aaataaaaaa aggttgccac aaaaaacatg gcgaaaggcc agagccattt tgaatttgat
      481 ttttgcatac cgcggcctag aataagccct tgcacaaggg acctaagaga agtatggtaa
      541 cgttctcagc tgactgtaat ggatgagtct agctcgacgc ccccataata gcaagaagtt
      601 tactttataa ctaacggact tagcaatgga tacaatggat ttagcttatc tctgtggatt
      661 tggaagttat tgggaaagcg aagcgcttcc aaacgcgttg ccaaaggggc aaaattcacc
      721 caaaaaaaat gcttatggtc tttataccga acaactaagc ggtagcgctt ttacccgatt
      781 gcgccacgaa aatttacgta cgtggctata tcgaatatta ccttccgtgt tacaagaaga
      841 ctttaccgcc tataaaggta ttgagtcttt ccatcacaat gcaagttatg cgcccccacc
      901 caaccagatg cgttgggatc cgcttcccat acctgacaaa cctctggatt ttatccatag
      961 cctccagcac gtactttata acggtgatcc atgtggacat ctaggtggag ctgcaattta
     1021 tgtattttgc gcgacaacct caatgcagga tacgtacttc tataacgccg atggagaatt
     1081 gcttattgtc ccttatctcg gcgcgatgcg tattcgtacc gagctgggta tattagaaat
     1141 tgtacctggg gaaatccttt gtgttccgcg cggagtaaaa ttctgtgctg aagtattggg
     1201 atccgttgcc gcaggctacg tatgtgagaa ttggggggct cccttcagct tgccttatct
     1261 cggccccatt ggttccaatg ggttagctaa cccaagagat tttaaaatac cgatggcaaa
     1321 ctatgaagat aaatccggag aatgtttatt aatttgtaaa tatttaaata atttttggca
     1381 agcgtcctta tcccattctc ctttaaatgt tgttgcttgg catggcaatt attcaccata
     1441 taaatacgac ttgcgtttgt tcaacacaat aaacaccgtc agttttgatc acccagatcc
     1501 aagtatattt accgttttga cctctcccac ctcaacccca ggattagcga atgtagattt
     1561 tgtgatcttc cccgaacgtt ggatggtagc agaacataca tttcgcccac cttactatca
     1621 tcgcaatatt atgagtgagt atatgggtct aatttatggc gtgtacgatg cgaaatcttc
     1681 agattttaaa ccgggcggag gcagcctcca taattgtatg tctgcacatg gtcctgataa
     1741 agaaactttc ttaaaagcgc aacaagattc tgaaagcccc tataaaatta cggatacctt
     1801 ggcatgcatg tttgaaagct ctaagccttg gattcctacg ccttgggcaa atcatacccc
     1861 cttacgtcaa aaaaattacg ttcactgttg gcaaaatttg cctaaactat ttaacaaata
     1921 atgaaagcag ctaataatag gcaacaaacc tatatagctg catcgaaatt aggtcaaagc
     1981 ttccttaagt ggttttaagg caattaattt aatatcatct cgagcgggtt tggagggtat
     2041 tgtgacttct tgtccggtta atggactaac catggtgcgt gctttggttc gattgcgccg
     2101 cacttttcgt attcgaatcc caagttttgg aagcaagatt tctcccgaac ctcttttaga
     2161 taagtgtgcc tttatcaacc ctgccattgc atcaaaaaca gcttcaacgt ctaccctttt
     2221 taaatttgtt ctctcagaga tatgctgcaa aatttccact cgagtttgtc tatctttaat
     2281 cggtttcgta agcatctttt tctccatttt tattaaagca aagttaacag attttaggtg
     2341 cgcgtattcc taccctattg agagtatata gtttcttctc cttctctttt gaaagaagtt
     2401 attgagatac atacatgagg caggaaatta tgccaagcct catggcttat gtaaaggcgt
     2461 gattatacaa gtgattgcaa acaactcgaa tcttcgaatt tcggattgtt cacccgggtg
     2521 ctcacgggat aagaagtaaa atgtggttgc gtcttcaaca aaagcgaaag cagggaatca
     2581 agaggtgtta gaggatctag ccattgcgca taaaaatcag aaggaatgac aataggactt
     2641 ctctcctgta aatccataaa ttgatctgca ctttttgtca caatagctac agtctctttg
     2701 atttcgaaat tagaattctt ccaagaatcc caaatgacag cgaaagctaa aataggaaat
     2761 tcaggcgcgt gcaaatagaa gggttgtttt ttatttttga taagcttcca ttcataatag
     2821 ccactcgcag gaatgagaca acgtcgctta cgaaaagaat ctttaaaggt gactttttca
     2881 tgcagtgtct cacatctagc attgatataa cgtgaagggg tctgtacatt tttgttggtc
     2941 cagctcggaa taaaccccca ttccataaat tgtaatgttg gaggatttcc gatta
//
LOCUS       DASTIB010000078         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_725079, whole genome shotgun sequence.
ACCESSION   DASTIB010000078 DASTIB010000000
VERSION     DASTIB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725079"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..785)
                     /locus_tag="VFP93_01340"
     CDS             complement(132..785)
                     /locus_tag="VFP93_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET9843285.1"
                     /translation="MDNLQKARLNMIHSQVRPWDVRDEKVLNMMNTIPREQFVPKEFE
                     DVAYSDCAIPLNETHKMLAPKVVARALQALAIQPTETALEIGTGTGYVTALLSHLANH
                     VYSIEIDPMLLNIAKRNLSQGYKNVTLELGNAVFGWQSHAPYAVIFVSGSYPLGLPTA
                     LETQILPGGRCFAIIGQEPNMQAVLYTRTSKRLKPEVLFETSSAALLHAPHPQEFTF"
     gene            908..1540
                     /locus_tag="VFP93_01345"
     CDS             908..1540
                     /locus_tag="VFP93_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9843286.1"
                     /translation="MKKKSTWEATLNRSDFECASTQTTHKGHYKVKILTLRHKLFSGE
                     YSAWISREFVLVPNSVAVLLYDPRQDLVVLVEQFRVGAIEATEHGPWLLECVAGYIEK
                     DETPEQAAARETLEEACCKIERLIPITQCYTTPGGCSEMTWIYCGLIDTNITDNVSKP
                     CDEEEDIKKHIIRYEQLMAYKHNLITNSATYIALQWLSIERDKLRDLSYL"
     gene            1563..1985
                     /locus_tag="VFP93_01350"
     CDS             1563..1985
                     /locus_tag="VFP93_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HET9843287.1"
                     /translation="MKHVRKSDVFLPNLTQYLNCCTSIYLKINALLPQNYRDSDSFCW
                     SVEPIANLTISIEERYRYTDAIQLKIFWIASTQWLTNFVIQLRMYHDLQIAEVIGYKT
                     KNKVIDFQLGDPKSKKNYIVEKKQIHHVLSEFLSLNFQ"
BASE COUNT          666 a          385 c          387 g          613 t
ORIGIN      
        1 gcttcttctg ctttaaaggt aggatcgttt tcaagggata aatgataaac ttctttaaga
       61 tccgcggcct gggcaaaatg ggaacccaga attattaatg ctgaaacaaa caatcgacgc
      121 ataattaatc tctaaaatgt aaattcttga ggatgtggtg catgcagcaa agcagcagaa
      181 gacgtctcga ataaaacttc aggttttaag cgtttcgagg tgcgcgtata taaaacagct
      241 tgcatattag gctcttgacc tataatcgca aaacaacggc cacccggtag tatttgcgtc
      301 tctagagccg taggcaaacc taatgggtaa gatcctgaaa cgaatattac tgcataaggg
      361 gcatgcgatt gccagccaaa aacagcattt cccaactcta atgtgacgtt tttatagcct
      421 tggcttaaat ttctttttgc tatattcaat agcataggat ctatttctat actgtataca
      481 tggtttgcta aatgcgaaag taacgcagtg acgtaccctg tgcctgttcc aatttctaaa
      541 gcagtttctg ttggttgtat ggctaatgct tgcagagcgc gagccaccac tttcggtgct
      601 agcattttat gcgtttcatt taaaggaatt gcacaatcac tatacgctac gtcttcgaac
      661 tctttaggta cgaattgctc tcgggggatg gtattcatca tatttaagac tttttcatcc
      721 cttacatccc aaggacgaac ttgactatga atcatattca agcgagcttt ttgtaagttg
      781 tccataaaag tgcgtttctt tgctttttgt tcgcatccta ccacactctg ggttttgcat
      841 ggaattttta gcaatctttg ctgaataacg gataatattt aaagtttaaa ggctgacaaa
      901 aaaatttatg aagaaaaaaa gcacatggga agcaacttta aatagatcag atttcgaatg
      961 cgcatctacg caaaccactc ataaaggtca ctataaagtt aaaattctaa ccttgcgaca
     1021 taaattattt tcgggagaat atagcgcatg gatttcacgc gaatttgtgc tagttcccaa
     1081 ctcagttgca gttttacttt atgatccgcg tcaagatctc gtcgttttag tcgaacaatt
     1141 tagagtggga gccatagaag ccaccgaaca tggtccatgg ttattagaat gtgttgcagg
     1201 atacattgaa aaagatgaaa cgcccgaaca agcagcagcg cgagaaactc tggaggaagc
     1261 ctgttgcaaa attgaacgct taatccctat tacacaatgc tacactactc cgggtggttg
     1321 cagcgaaatg acatggattt attgcgggtt aattgacaca aatataaccg ataacgtaag
     1381 caaaccctgt gatgaggagg aagatataaa aaaacatatc attcgatacg aacaattaat
     1441 ggcttataaa cataatttga taaccaattc tgctacctat attgcccttc agtggctgag
     1501 tatagaacgt gataaactac gggatctgag ctatctttaa taaaagaata acctcgatcc
     1561 aaatgaaaca cgtaagaaaa tcggatgtat ttttacccaa tctcacacaa tatttaaatt
     1621 gctgtacaag tatttattta aagataaatg ctttgttgcc acaaaattat cgcgatagcg
     1681 atagcttttg ttggtctgtc gaacctattg caaatctcac tatatctatt gaagagcgat
     1741 atagatacac cgatgcaata cagctaaaaa ttttttggat agcttctacg cagtggctga
     1801 caaattttgt cattcaatta cgcatgtatc atgatttaca aattgccgaa gtaataggat
     1861 acaaaacgaa aaataaggtt atcgattttc aactgggcga tcctaagagc aaaaaaaatt
     1921 atatagtaga aaaaaagcaa attcatcatg tattatctga attcctttcc ctgaattttc
     1981 aatgataaat ctgcttctta ctcacagcct tgacattatt agattatagt acatgaatgg
     2041 ctaacttcga a
//
LOCUS       DASTIB010000079         4745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_778669, whole genome shotgun sequence.
ACCESSION   DASTIB010000079 DASTIB010000000
VERSION     DASTIB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778669"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..815)
                     /gene="surE"
                     /locus_tag="VFP93_01355"
     CDS             complement(48..815)
                     /gene="surE"
                     /locus_tag="VFP93_01355"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9843288.1"
                     /translation="MRILLSNDDGVNAPGLRVLAKSLEKLGHIKVVAPDRDRSAASHS
                     LTIDAPLRMTELENGFVAINGTPTDCVHLAIRGYLKEAPDMVVSGINSGSNLGDDVLY
                     SGTVAAAIEGRFLGYPTIAVSLAGVEGGELTHYETAGWYIARIVSELFANPLHKDVIL
                     NVNVPDLPLEQTKGIQVTRLGLRHLAEGVLTTKDPRGKVVYWVAPAGPGSDAGPGTDF
                     YAIQQGYVSVTPLKTDLTDKQQLPILEEWFSMLKLNR"
     gene            1218..2012
                     /locus_tag="VFP93_01360"
     CDS             1218..2012
                     /locus_tag="VFP93_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843289.1"
                     /translation="MFSIQELQKQGLAWKTMAHLTEPHDINGTLKPYVIKIEIALKNY
                     PMISPFNFYFSDKKDFERFHNIFAVLGEKVYNNYLADAGSFPKETEQLVFAQKPTPIL
                     VTLKTIDVPENDYPFCKKLLMNALKANPNIVSLRLPKSLLMTNTPLKIKEDSFQSNLF
                     FSAVFMGFAFIIAWTCTPLTMTGLLMLSTCFGVGCFSLTSALKNFSTNHDEEIKKEAI
                     GKCQESNFIEEQKKALFFQQPKTAINTGETNQTEVQPHRSARVRLG"
     gene            complement(2080..2589)
                     /gene="ispF"
                     /locus_tag="VFP93_01365"
     CDS             complement(2080..2589)
                     /gene="ispF"
                     /locus_tag="VFP93_01365"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009669542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET9843290.1"
                     /translation="MKIRIGHGFDLHRVQPADQESSGIMLGGVLVPYHQTIVAHSDGD
                     VILHAITDALLGALGLGDIGQWFPDTDPKYKNVSSDRFLLEVYAKVRSMHYTINNIDV
                     TVIAQKPKLKNYILAIREKIADLLKISVNDISIKAKTHEGCDSIGEEKALAAHAVVLI
                     CSDESIKHS"
     gene            complement(2586..3284)
                     /gene="ispD"
                     /locus_tag="VFP93_01370"
     CDS             complement(2586..3284)
                     /gene="ispD"
                     /locus_tag="VFP93_01370"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET9843291.1"
                     /translation="MQANISVVIAGAGIGARFGVNLPKQYHKIGNKTVLEHSIKSMQQ
                     VFTQSTMVVALNPHDQWFHTLEIANDSRVIPIRGGETRGESIWHALEFLKKSPTPWVL
                     IHDAVRPCVPQSDIERLVKLAQSHPPGGILAAPSIDTLKRVQHKKITQTVNRNLIWRA
                     LTPQLFEFDKLYFAYEKARKEEIEITDDAYVMELAGLSPYVVEGSSINIKITYPHDLK
                     VASSYLQAKEEICL"
     gene            complement(3274..3543)
                     /locus_tag="VFP93_01375"
     CDS             complement(3274..3543)
                     /locus_tag="VFP93_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET9843292.1"
                     /translation="MKFLLGALVILLVLLQIRLWTGDGGVVKVVKLKEEVNTQKEQTL
                     ELSERNHILEAEVQDLKNRLDALEERARTDLGMIKQGETFYQDAS"
     gene            complement(3540..>4745)
                     /gene="eno"
                     /locus_tag="VFP93_01380"
     CDS             complement(3540..>4745)
                     /gene="eno"
                     /locus_tag="VFP93_01380"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9843293.1"
                     /translation="ENGVLGRASVPSGASTGSREAIELRDHDHRYAGKGVLKAVKNIE
                     EVIQAAIKGTNVQDQKKIDKTLIALDNTENKSKLGANALLAVSLACAKASAKANQIPL
                     YQYLASLLDSKTYTMPVPLMNILNGGEHADNNVDIQEFMIVPFAADSFSEALRFGTEI
                     YHALKKLLKQKGLSTAVGDEGGFAPNLSSNQQALDLIMHTLNDVGLKPGKEVSIALDV
                     AASEFYENGLYHLTSEKRQLTGEALINLYEQWIKMYPIISIEDGLAENDWEHWRLLTQ
                     RLGAQTQLVGDDLFVTNPIIFRDGIQYGIANAILIKLNQIGTLTETLETIQLAKEYNY
                     KAVISHRSGETEDTTIADLAVASGVGQIKTGAPCRTDRVAKYNQLIRIEEALQAPFAG
                     LRAFNKSSV"
BASE COUNT         1405 a          993 c          847 g         1500 t
ORIGIN      
        1 tatcatttta ttatataacc gactaaacat aggtggcttc ctaaaaacta tcgattgagt
       61 tttagcatac taaaccattc ttctagaatt ggtaactgtt gcttatcggt taaatcggtt
      121 tttaaagggg taaccgatac atatccttgt tgaattgcat aaaagtcggt accaggaccc
      181 gcatctgacc ctggccccgc aggtgctacc caataaacca ctttgccgcg tggatctttt
      241 gtagtaagca cgccttctgc taagtgtctt agccccaacc gagtaacttg tatgccttta
      301 gtttgttcca acggtaagtc tggtacgttc acgtttaaaa taacatcttt gtgtaagggg
      361 ttggcgaata attcgctgac aattcgcgcg atataccaac ctgcagtttc atagtgggtt
      421 aattcacctc cctctacgcc tgcaagagaa acagcaattg tcggatagcc taaaaatcta
      481 ccttctatcg ctgctgctac cgtgccagag tacaaaacat catcacccaa atttgaaccc
      541 gaattaattc cagaaactac catgtcggga gcctctttga gatagccacg aattgctaaa
      601 tgcacacaat ctgttggagt gccgttaatt gcaacaaagc cgttttctaa ttctgtcatg
      661 cgcagtggtg catcgatggt taaagaatgg cttgcggcac ttcgatccct atctggtgct
      721 acgactttta tatggcccaa tttctcaagt gacttagcca acactcttaa gcctggcgca
      781 tttactccat catcgttact taacaaaata cgcataaact ttctacaatt aacccgcgtt
      841 ttattttaat tcagtatatt gaccaggggt ttattccgca acaataactt ttattcatct
      901 aacttaatct ccggtttagt aattacataa agatttataa tcaatatagc tattaattca
      961 tttgacttac ccgactaatt tcatacgcga agtgcatttt ttttacttct gattttttat
     1021 ttagtaaaat acacaaaaat gcatagcaga attttatgta ggaacagaga gctttatgat
     1081 agcaatcgat cagttaaaaa accctaaaac acccgcaggt aatgtaaaac cttataaaat
     1141 aagtttcatt gaaacgctcg catacgaaaa tgcaataaaa tttttttaaa tttcttataa
     1201 atgggggaaa actaaagatg ttttcgattc aagaattaca gaaacaaggt ttagcctgga
     1261 aaaccatggc acaccttact gaaccacatg atatcaatgg tacattgaaa ccttatgtga
     1321 ttaaaattga aattgcgctc aaaaattatc ccatgatttc cccatttaat ttctattttt
     1381 ctgacaaaaa agactttgaa cgttttcaca atatttttgc tgtactaggt gaaaaagttt
     1441 acaataatta tttggcagat gcaggttcat ttccaaaaga gactgaacaa ttagtatttg
     1501 ctcaaaaacc aacccccatt ttggtcactt taaaaaccat agatgttcct gagaatgatt
     1561 atccgttttg taaaaaattg ctaatgaatg cattaaaagc aaacccaaat atcgttagtt
     1621 tgaggctacc gaaaagtcta ttaatgacaa atacaccact aaaaataaaa gaagatagtt
     1681 ttcaatctaa tttatttttc agcgctgtgt ttatgggttt tgcatttatc atagcttgga
     1741 cttgcactcc tctaacgatg actggtctgt tgatgttaag tacgtgcttt ggcgtaggct
     1801 gtttttcatt gactagtgca ttaaagaatt tttctacaaa tcatgatgaa gagattaaaa
     1861 aagaggcaat tggaaagtgt caagaatcaa attttataga agaacaaaaa aaggctttat
     1921 tttttcaaca acctaaaact gcgatcaaca caggggaaac caatcagact gaagtgcaac
     1981 cgcatcgttc tgcgcgtgtt cgtttaggat aaagactgga tgccgcgaat taaagtcgcg
     2041 gcacgtaggc aatggaaaag cgtaggcgga taggtttaat tacgaatgct taatcgattc
     2101 gtctgagcaa atcaatacaa ccgcatgcgc tgctaatgct ttctcttctc caatactgtc
     2161 gcaaccttca tgggtttttg ctttgatact aatatcatta accgaaattt ttaaaagatc
     2221 ggctattttc tcacgtatag ctaaaatata atttttaagt tttggttttt gcgcgataac
     2281 ggtcacgtct atattgttaa tggtataatg catggatcgc acttttgcgt aaacttctag
     2341 taaaaagcga tcgcttgaaa catttttata tttaggatcg gtatccggaa accattgtcc
     2401 tatatcgcct aagcctaacg cccccaacaa agcgtcggta atggcatgta aaattacatc
     2461 gccatctgaa tgcgcaacga tggtttggtg atagggaacc agaacgccgc ctagcatgat
     2521 cccagaagat tcttggtctg caggctgtac tcgatgcaaa tcgaaaccgt gtccaatgcg
     2581 tattttcata agcaaatttc ttctttcgct tgtaaataag aggaagcgac ttttaaatca
     2641 tgtggatagg ttatttttat attgatacta ctaccctcta cgacataagg agaaaggccc
     2701 gctaattcca tgacataagc atcatcggta atttcaattt cttcttttcg tgctttttca
     2761 taagcaaaat ataatttgtc aaattcgaat aattgtgggg ttaacgcgcg ccatattaaa
     2821 ttgcgattca ctgtttgagt gattttttta tgctgcaccc ttttcaaggt atcaatagaa
     2881 ggagcggcga gaattccacc tgggggatga ctttgcgcta atttaactaa acgttcaata
     2941 tcactctgcg gtacacatgg gcgcactgca tcatgaatca atacccaagg ggtaggcgat
     3001 ttttttaaaa attcgagcgc gtgccaaatg gattccccgc gggtttcacc tccacgaata
     3061 ggaatcactc tggaatcatt agcgatttct aatgtatgga accattgatc gtgtggattt
     3121 aaagcaacga ccatggtgct ttgggtaaaa acttgctgca tcgattttat cgaatgctct
     3181 aaaaccgttt tattaccaat tttatggtat tgctttggaa gatttacccc aaaacgtgct
     3241 ccgatccctg cacccgctat cacgacacta atattagctt gcatcttgat aaaatgtctc
     3301 cccttgttta atcataccta aatcggtgcg cgctcgctct tcgagcgcat ctagccgatt
     3361 ttttaaatcc tgaacctctg cttctaaaat atgattacgc tctgataatt cgagtgtttg
     3421 ttccttttgg gtattcactt cttcttttag cttaacaact tttacgactc caccatcccc
     3481 ggtccaaaga cggatttgca ataacactaa aagtataaca agtgccccta atagaaattt
     3541 cataccgaac tcttgttaaa agcacgcaaa cctgcaaatg gagcttgcaa agcctcttca
     3601 atacgtatta gttgattata tttcgctact ctatcggtac gacacggtgc accggttttt
     3661 atttgaccca caccacttgc aactgcaaga tctgcaatgg tcgtatcttc ggtttcaccc
     3721 gagcgatgcg aaattactgc tttataatta tactctttgg ctaattgaat ggtttctaaa
     3781 gtttctgtta gagttccaat ttggtttaat ttgataagta tggcatttgc aataccatat
     3841 tgaattccat cccgaaagat aattgggttg gttacaaata aatcatctcc aaccaactgg
     3901 gtttgtgcac ctaaccgctg cgtcagtaac cgccaatgtt cccagtcgtt ctcagccaac
     3961 ccatcttcaa tagaaatgat cggatacatt ttgatccatt gttcataaag attgattaat
     4021 gcttcacccg ttaattgacg tttctctgaa gttaggtgat ataggccatt ttcataaaac
     4081 tcacttgccg caacatcgag tgctatcgat acttctttac ctggtttcaa cccaacatcg
     4141 tttaacgtgt gcatgattag atccagcgct tgctgatttg aggataaatt aggcgcaaat
     4201 ccaccttcat ctcccactgc agtactcaaa cctttttgct taagtagttt ctttaacgca
     4261 tgataaattt ctgtaccgaa gcgtaaagct tcactaaaag aatcagcagc aaagggtacg
     4321 atcatgaact cttgaatatc aacgttgtta tccgcatgtt ctcccccatt tagaatattc
     4381 ataagaggaa ccggcatagt gtacgttttc gaatcaagaa gagaagctaa atactgataa
     4441 agcggtattt gatttgcctt ggcacttgcc tttgcacaag ccaaagaaac cgctaacagt
     4501 gcattggcac cgagtttact cttattttcg gtattatcta gcgcaataag ggttttatcg
     4561 atcttttttt gatcttgcac gtttgtgccc tttatggctg cttgtataac ctcttctata
     4621 tttttaacgg cttttaaaac accctttcct gcataacgat gatcatgatc ccgcagttca
     4681 atcgcttctc ttgatccagt tgacgcaccc gatggcacag atgctcgacc taatacacca
     4741 ttttc
//
LOCUS       DASTIB010000080         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1362088, whole genome shotgun sequence.
ACCESSION   DASTIB010000080 DASTIB010000000
VERSION     DASTIB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362088"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFP93_01385"
     CDS             <1..343
                     /locus_tag="VFP93_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9843294.1"
                     /translation="PVKLRTIVQALDDKTHTQEVRLSFESAKPLPGTFGILTWQDSKP
                     YLPAEFISQRNNSLGLFVNKNNKAHFMPIPNALEGQPIPLTSVIKENIIIEGRHSLED
                     GVALLLTNNKG"
     gene            346..>2462
                     /locus_tag="VFP93_01390"
     CDS             346..>2462
                     /locus_tag="VFP93_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9843295.1"
                     /translation="MRFLKIWLSNHVLANLSFVLVLLLGTLTYFNLPREQDPTINFNW
                     VQISTLFPGASAEDVEKRVTEILEDAIRTISDIRFVSSVSRDSISSILVRFHDISEHA
                     FEKKLVDLRREIQNKERLLPQGAQKPEVIEITSSNAFPSATIAIVGKADDNQLRTIAR
                     NVRKDLERLHGVDRVDALGLRDAELQSYFLPNHLAEYGITSNDIANSIAQNYQDIAAG
                     SILIENARWLVRWLGTSADPSYLNQIPITHAHEDLTLAEVAKIQQGREKPSQLVLYEG
                     KNAIILTVMKKGNANILKLVDTIQDYVHNMNAVQKNTGVKMVLLDDQTQITKNALSIM
                     ENNAWFGLLLVAFTCWIFLGFKISLLISLGIPFSLCATFLCLDGLNQTLNVSVLLGLV
                     IALGMLVDDAVVVVEAIQYRLERGFDKMRATLDGLQEVFAPVCSSVMTTIAAFLPLML
                     MPGILGKFMLVIPMVVCLALIFSLLEAFWMLPAHILNMHVNSSRQQWRRKMNQKIRFF
                     YTRILMKSFRYPKVTLLSIFLLFSFSCIALSTNLIKFNFFASDPMRLFYINVEMHSGT
                     PITETLRFTEQIENTIRPFLQKDEARSIASYAGIMFTETEPVLGEQYGQVIISLHPQQ
                     ENMRSVAQIIDSMRLAAVSKPGPKKITFMPLAGGPPTSKPINIKIRGDNFATIRAALV
                     DVKTILQSIPSLTDIADNDTVGQP"
BASE COUNT          745 a          471 c          475 g          771 t
ORIGIN      
        1 ccctgtgaag cttagaacta ttgtgcaagc actagatgac aaaacgcaca cccaagaagt
       61 tcgcttaagt tttgaaagtg ctaagccact gcctggaact tttggcattt taacttggca
      121 agattccaaa ccctatttac ctgctgaatt tataagtcaa cgcaacaata gcttaggttt
      181 atttgtaaac aaaaataata aagcgcactt tatgccaatt ccaaacgctt tagaagggca
      241 accgataccc ttaaccagcg ttattaaaga aaatattatt atagaagggc ggcatagttt
      301 agaagatggc gttgctttgc tattaaccaa taataaagga taaacatgcg gtttctcaaa
      361 atatggttaa gcaatcatgt tttagccaat ctttcttttg ttcttgtgct tttattagga
      421 acgcttacgt atttcaattt accgcgcgaa caagatccaa cgataaattt taattgggtg
      481 caaattagca cgctatttcc gggcgcctca gctgaagatg tggaaaaacg cgtaacagaa
      541 attttggaag atgcaattcg cactatttct gatatacgtt ttgtatccag tgtttctcga
      601 gatagcatct caagcatctt agtgcgtttt catgatattt cagaacacgc tttcgaaaaa
      661 aaattggtgg atctgcgcag agaaattcaa aataaagaaa ggttgttgcc gcaaggcgcc
      721 caaaaacctg aagtaataga aattacctct tccaatgcct ttccttcggc tactatcgct
      781 atcgtaggca aagccgacga taatcaatta cgaaccatcg ctagaaatgt gcgtaaagat
      841 ttagaacgcc tacatggtgt ggatcgcgtc gatgcattag gactacgaga tgctgaatta
      901 caaagttatt ttttacctaa tcatttagca gaatatggta ttacgagtaa cgatattgcc
      961 aatagtattg cccaaaatta tcaagatatt gctgccggtt ctattttaat tgaaaatgcc
     1021 agatggttag tccgttggtt aggaacgagc gcagatccta gttacttaaa tcaaattccc
     1081 attacccatg cgcatgaaga tctcacactc gctgaagttg ccaaaataca acaaggacga
     1141 gaaaaaccct ctcaacttgt attgtatgaa ggaaaaaacg ctatcatctt aaccgtgatg
     1201 aaaaaaggaa atgcgaatat cttaaaatta gtcgatacca ttcaagatta tgttcataat
     1261 atgaatgccg tacaaaaaaa tactggcgta aaaatggtgc tcttggatga tcaaacccaa
     1321 atcacaaaaa atgcattaag tatcatggaa aacaacgcgt ggttcggtct tcttttggtt
     1381 gctttcacgt gttggatatt tttagggttt aaaatttctt tgcttattag cttagggatc
     1441 cctttctctt tatgtgctac ttttttatgc ttagatggct taaatcaaac cttaaacgtg
     1501 tcggttttat tggggcttgt aattgcttta ggcatgcttg tagacgatgc cgtggtggtg
     1561 gttgaagcca ttcaataccg cttagagcgt ggttttgata aaatgcgcgc aactttagac
     1621 gggttgcaag aagtatttgc gccggtgtgt tcctcagtaa tgacaacgat tgcagctttt
     1681 ttaccgttaa tgctcatgcc aggtattctt ggtaaattta tgctcgtaat tcctatggtg
     1741 gtttgtcttg cattaatttt tagtttactc gaggcgtttt ggatgctgcc tgcccatata
     1801 ttaaacatgc atgttaattc gagtaggcaa caatggcgca gaaaaatgaa tcaaaaaata
     1861 cgtttttttt atacacgtat tcttatgaaa tcttttcgtt atccgaaagt aactctgctt
     1921 agtatttttt tactcttctc cttttcatgc attgctttgt ctacgaatct tattaaattc
     1981 aatttttttg cgtcggatcc gatgcgactt ttttacatca atgtggaaat gcatagtggt
     2041 actccgataa cggaaacgtt acgttttacc gaacaaatag aaaatactat tcgtcctttt
     2101 ttgcaaaaag atgaggcacg atctatcgct tcttatgcgg gtattatgtt tacggaaaca
     2161 gaaccggttt taggcgaaca atatggtcaa gttataatca gtttgcatcc ccaacaagaa
     2221 aatatgcgtt ccgttgcaca aattatcgat tccatgcgtc ttgccgccgt atccaagcca
     2281 gggccaaaga aaattacttt catgccctta gcaggtggcc cacctacttc caagcctatc
     2341 aatataaaaa ttcgcggaga taactttgca acgattcgtg cggcattagt agatgtaaaa
     2401 actattcttc aatctatacc atcactgacg gatattgctg ataacgatac cgttgggcaa
     2461 cc
//
LOCUS       DASTIB010000081         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_733837, whole genome shotgun sequence.
ACCESSION   DASTIB010000081 DASTIB010000000
VERSION     DASTIB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733837"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFP93_01395"
     CDS             complement(<1..208)
                     /locus_tag="VFP93_01395"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012155803.1"
                     /note="catalyzes the formation of
                     1-(5-phosphoribosyl)-5-aminoimidazole from
                     2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in
                     purine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET9843296.1"
                     /translation="METATTYKNAGVDIDAGNALVNRIKKHVAKTIRPGVIGQLGGFA
                     GFFELPTHYRQPILVSCTDGVGTKL"
     gene            369..1427
                     /locus_tag="VFP93_01400"
     CDS             369..1427
                     /locus_tag="VFP93_01400"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HET9843297.1"
                     /translation="MKLRKYLILYILFDLFSLSTCWALDSENLYEVQLIAISKEEGER
                     LELMREGLRQTFVRISGKKEVLKEVAIQQALKNPLPYVTQYQYQQDMFRVLYNRKLIS
                     ALLNQTASTVLQDERPSMIVWISMEVEDDRRWVGIEQDPSIVALVEKIASSRAQPIVF
                     PLLDLEDLEKMNLKRSAITLGDTQHASKRYDADILLVGRLIQNKDQKNWEGDWKLFTP
                     KKDAHFKTEIAPLNQTVTQGMEKMMDTMTQLYTAPDTENLTQASSFIIQIENIRTGGD
                     HAEILRFLKNIPSIDDVEVLSITPTRTAFQITPKANRSATTILHALKTNPQLTMRDSS
                     FMHAENKEEITYQWKGQI"
     gene            1424..>2281
                     /locus_tag="VFP93_01405"
     CDS             1424..>2281
                     /locus_tag="VFP93_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9843298.1"
                     /translation="MNDAKKAIIFLSTLISLIILGWLILKLTPVLMPFLLAVGIAYLA
                     DPLVEKLTTIPRIPRTLAVSFVFTVILFLFLMFLLFVVPILNRETVEFVKIIPQVFLR
                     LQEIILPRVEEFTGLSLANYDIQSFQETLMKHWTKATNFMTWLGQWLFHSGMTILHWG
                     INSILIIVVTFYLLRDWHKMLHSLKYLIPISYRHQVLVLIKKSDTVMGTFLRGQLSVM
                     LVLSVIYAIGLTLIGVPFSVLLGIISGLLSIVPYFGSIIGFLSTVIISLVQNQEGWNL
                     IWIAIVFGIG"
BASE COUNT          701 a          410 c          440 g          730 t
ORIGIN      
        1 gtaatttggt gccaacacca tctgtgcagc taactaaaat gggttgtcga taatgggtag
       61 gcaattcaaa aaaccccgca aaccctccta actggccgat aacaccaggg cgtattgttt
      121 tagcaacatg ctttttaatt ctattaacta acgcatttcc ggcatcaata tctacaccag
      181 catttttata ggtagtggca gtttccatgg taaatccttc aatttaatca cttattctag
      241 caatcttact aaacgttgta gctattaacc ataagttact ggatcatcta agccttcgca
      301 aataatagat tttcgcggtt aggcagtaat tgcggcataa taatgtatca attcgatgaa
      361 ggtgcacgat gaaattacgc aagtatctaa ttctttatat actttttgat cttttttctt
      421 taagtacctg ttgggcactt gattctgaaa atttgtatga agtgcaactt attgccataa
      481 gtaaggaaga gggtgaacgt ttagaattaa tgcgtgaggg attgcgtcaa acctttgtaa
      541 ggataagtgg aaaaaaagaa gttttaaaag aggttgccat ccagcaggct ttaaaaaatc
      601 ctctacctta tgttacacaa tatcaatacc aacaggatat gtttcgcgtt ctgtataatc
      661 gcaaattgat tagtgcatta ttaaaccaaa cggcatctac cgttttacag gatgaacgtc
      721 cctcgatgat cgtatggata agtatggaag tggaagacga tcgacgttgg gtaggtatag
      781 agcaagatcc aagtattgta gctttagtgg aaaagatagc atcgtctcga gcccaaccta
      841 ttgtatttcc tttgttagat ctagaagatt tggaaaaaat gaatttgaaa cgcagtgcta
      901 tcactttggg cgatacgcag catgcctcaa agcgttatga tgcagatatt ttgttagttg
      961 gacgtttgat acaaaataaa gatcagaaga attgggaagg ggattggaaa cttttcacgc
     1021 caaagaagga tgcacatttt aaaacggaga ttgcaccgtt gaaccaaacg gtaacgcaag
     1081 gtatggaaaa aatgatggat actatgacgc agctctacac tgcacctgat acggaaaatc
     1141 tcacccaagc ttcttctttt attatccaaa ttgaaaacat tcggaccggt ggtgatcacg
     1201 ctgaaattct ccgatttttg aaaaatatcc catcgattga tgatgtggaa gtgttgagta
     1261 ttactcctac ccgtacagcg ttccaaatta cgcccaaagc aaatagatcc gctaccacta
     1321 ttttgcatgc cctaaaaacg aacccacaat taactatgcg cgatagctca tttatgcatg
     1381 cggaaaacaa ggaggaaata acgtaccaat ggaaaggaca gatatgaatg acgcaaaaaa
     1441 agcgataatt tttttatcca ctttaatcag cttaataatt ctgggatggt tgatattaaa
     1501 acttactccc gttttaatgc ctttcttgct tgcagtggga attgcctatt tggcagaccc
     1561 tttggtagaa aaattaacca ccattcctcg cattcctaga accttagctg tttcttttgt
     1621 ttttacggta atcttatttc tattccttat gtttttatta tttgttgtgc cgattttaaa
     1681 tcgagaaacc gttgaatttg taaaaattat tccacaagtt tttttacggt tacaagaaat
     1741 aattttacct cgggtagaag agtttacagg tcttagtctt gcgaattatg acatacaatc
     1801 cttccaagaa actttgatga aacattggac taaagccaca aattttatga cttggttggg
     1861 acaatggcta tttcattcag gcatgactat cctacattgg gggattaaca gcattttaat
     1921 tattgtagta actttttatt tgctacgaga ttggcataaa atgctgcaca gcttaaaata
     1981 tttaattccc atttcttatc gacatcaggt ccttgtactt atcaagaaaa gcgataccgt
     2041 catgggtaca tttttgcgtg gacaactttc tgtcatgtta gtattaagtg ttatttacgc
     2101 gatcggattg actttaatcg gagtcccttt ctcagtacta ctgggcataa tttctggttt
     2161 attaagcatt gtaccctatt tcggaagtat catcggattt ttaagtaccg tgattatctc
     2221 tttagtgcaa aatcaagaag gctggaatct tatttggatt gcaatcgtat ttggaatagg
     2281 t
//
LOCUS       DASTIB010000082         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_732890, whole genome shotgun sequence.
ACCESSION   DASTIB010000082 DASTIB010000000
VERSION     DASTIB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732890"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFP93_01410"
     CDS             complement(<1..927)
                     /locus_tag="VFP93_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843299.1"
                     /translation="MTFSEILEKLHKILKLNSYGNNDACDQAQQLVNELKNSVNSDIY
                     SLITPIIEKSQDSNFAIEIPESAQNFSNYFDPSVYQELLNYTTLAHYFEPNGIPEEQA
                     LKLSVLFGDCEAALKYISNNKSSLHDACLFQLPPVEECNFSLWQQHAKKSMLDYRFRK
                     LLPNANEIEKLMFSAKKQKLDYQAINAKKNEINHVHREWKNLKRKHGTLNNAEKGRYQ
                     QLGKDLFQLNEELVPLCSGISLHNADLVILNAFYQHYIKTAHPSYQYFLQHGLTSKDY
                     DKFVALNREQAGKNIPDLIIDGTEIGYPGFYLM"
     gene            1309..2034
                     /locus_tag="VFP93_01415"
     CDS             1309..2034
                     /locus_tag="VFP93_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HET9843300.1"
                     /translation="MRGATTQTVFEEEDSNFITEYAPLVKRIAHHLLGRLPQSVQVED
                     LIQAGMIGLIEAFRNFDANKGASFTTYAGIRIRGSMLDEIRKGDWAPRSVHRNSRRVA
                     EAIREVEHHLGRDAKPKEVAKILGITVEEYHQIMQDSNSVKIFAFEELGVNEDAFMHP
                     HTGIAGPLDGLQKEDFKEHLAKEIAKLPEREKLVLALYYDEELNLREVGDVLGVSESR
                     ISQIHSQAMNRLQARMKSWKPSD"
     gene            2099..2497
                     /locus_tag="VFP93_01420"
     CDS             2099..2497
                     /locus_tag="VFP93_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843301.1"
                     /translation="MKDVQYLVFSAADYIFTCAMNDVTEILPVRDMLPTELNDEQILG
                     YIKRKSGIFIPVVDVVYFYFKQNCKYPCNEIVLLDTSTRVVGCGAQQILAVSQAREWD
                     ALKSCIHPQRCNTSYRPMPIEKDTLNLALQ"
BASE COUNT          814 a          437 c          534 g          822 t
ORIGIN      
        1 cattaaataa aacccaggat atcctatctc tgtgccatct attattaagt caggaatgtt
       61 tttacctgct tgttccctat ttaatgcaac aaacttgtcg taatctttac tggtgagtcc
      121 atgttgcaga aaatattgat aagaggggtg tgcggttttg atataatgtt gataaaaggc
      181 atttagaatc actaaatcgg cattatgtaa agagatgccc gaacaaagag gtactaattc
      241 ttcgtttaat tgaaataaat ctttacctaa ctgttgatac ctacctttct ctgcattgtt
      301 cagcgtgcca tgttttcgct taagattttt ccattcacga tgtacatggt ttatttcatt
      361 cttttttgca ttaatagctt gataatcgag tttttgtttt ttggcagaaa acatcagttt
      421 ttcgatttcg ttcgcattag gaaggagttt acgaaagcgg taatccagca tggatttttt
      481 agcatgctgc tgccataaag aaaaattgca ttcctctaca ggaggtaatt gaaataagca
      541 agcatcatgg agtgaacttt tattgttgga aatatatttt aacgcagcct cacaatcacc
      601 gaataatacc gataatttta atgcctgctc ttcgggtatc ccgttgggtt caaaataatg
      661 cgcaagcgtt gtatagttaa gtagttcctg gtaaacgctt ggatcaaaat agttgctgaa
      721 attctgcgct gactctggaa tctctattgc gaaattggag tcttgcgact tttcgattat
      781 tggggttatt aaagaataaa tatcgctatt aactgaattc ttcaattcat ttacaagttg
      841 ttgcgcctga tcacacgcat cattattacc gtaagaattt aattttaaga ttttgtgaag
      901 tttttcaaga atttcagaaa aagtcattgg attacccgaa tattttagtc aaatacctga
      961 tgtatcctaa gtgggggata tataagtttc aagaattaag tttaattttt ggatgaatat
     1021 tacaaagtgt cagatgcatg gtttttttcg cgggattgga atttaccaga gtcaggctct
     1081 atgaatcgat tcgttttttg aattaaataa cgtcactgga caaatttttt tcaaattttt
     1141 tgatacaaag ttacaacaga gtgagctcta ttgagttttt ttgacaaatc cgagtaatta
     1201 cgtctttcct tgcgttaagg atttctttgc taagctaaat atagaaactt taattgaggc
     1261 cgataacttg cacgcattgg taagctgcaa gagggaggaa gtaactaaat gcggggagct
     1321 acaacccaga ctgtattcga ggaagaagat tctaatttta ttacagaata tgcgccactg
     1381 gttaaaagaa ttgcccatca cttattaggt cgtttaccgc aatctgtgca agttgaagat
     1441 ttaatccaag caggcatgat tgggcttatt gaagcctttc gaaattttga tgcaaataag
     1501 ggggcaagtt ttaccaccta tgcggggatt cgtattcgag gctctatgct ggatgaaatt
     1561 agaaaagggg attgggcacc gcggtcggtt catcgaaatt ccagacgtgt agcggaagct
     1621 attcgagaag tagaacacca cctaggacga gatgccaaac ccaaagaagt agccaaaatt
     1681 ctagggataa cggtagagga ataccatcaa attatgcaag attctaacag tgtgaaaatc
     1741 tttgcatttg aggagttagg ggtgaacgaa gatgcattta tgcaccccca tacaggtatt
     1801 gcaggtcctt tagatggttt gcaaaaggaa gattttaaag aacatcttgc caaagaaatt
     1861 gctaaattgc ctgagcgcga aaaattagtg cttgcccttt attatgacga agagctcaat
     1921 ttacgcgagg taggggacgt attaggagta agtgaatcgc gtatcagtca aatccattcg
     1981 caagcgatga atcgcctcca agctcgcatg aaatcatgga agccatctga ttagctgaat
     2041 ttctcacaaa ttaaactaca cttatcgtga aagaagattt cataaagaag gatattttat
     2101 gaaagacgtg cagtatttgg tcttttcagc agctgactat atttttacat gtgcaatgaa
     2161 cgatgtgaca gaaatattgc ctgtgcgcga tatgcttcct acagaactaa atgacgaaca
     2221 aatcttaggg tatatcaaga gaaaaagcgg aatttttatt cccgtagtgg atgtagtgta
     2281 tttttatttc aaacaaaatt gcaaatatcc ttgtaacgaa atcgtcctgt tagacaccag
     2341 cacccgagta gttgggtgcg gtgcacaaca aattttggcg gtgtcgcaag ctcgagaatg
     2401 ggatgcttta aaaagttgta ttcatcccca acgctgcaat acaagctatc gtcctatgcc
     2461 gattgaaaaa gatacactca atctcgcttt gcaataaatg gaaatttaac attttgccac
     2521 ccttgtgtaa aaagattaaa aaataactct gaacttcttt tggtaagcaa agatagagaa
     2581 gaagataatt tatcttttaa tgtatgt
//
LOCUS       DASTIB010000083         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_322302, whole genome shotgun sequence.
ACCESSION   DASTIB010000083 DASTIB010000000
VERSION     DASTIB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322302"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFP93_01425"
     CDS             complement(<1..602)
                     /locus_tag="VFP93_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843302.1"
                     /translation="MQVKKSVNGFCLALCGLILTFGFENTMANTAETLLVFDNENYTE
                     LPKNWREPIGLVKKETPFAFLEGLNKLQASASGQFSETTLNTAFPKHDRAKVWIIDLR
                     GEAHGFLDGQAVSWYGYRNLALQNLSLQELLQEETRLLESLKKQDTITVYQIEKKVGG
                     EIQQVQERMVKPQRVESEAQLTKRLGFHYKRFVVLDRHPPS"
     gene            758..1273
                     /locus_tag="VFP93_01430"
     CDS             758..1273
                     /locus_tag="VFP93_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843303.1"
                     /translation="MGKWFVVLISFVSLNVSAQETRLYACKQGDGTVLVQDKPIDNCA
                     QEKIFHYETQDAAKEPQGLREAEIKLLDESQSQQILINRYQNVDETVGNSLSIREKDR
                     RHDNCYLYRSTIETALINIDDLEHRPSDDLFVRQFIAGLERDNAQHKYYCGQEYPQLK
                     DFIQRYKSRFF"
     gene            1396..2028
                     /locus_tag="VFP93_01435"
     CDS             1396..2028
                     /locus_tag="VFP93_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET9843304.1"
                     /translation="MHLQKILLGVTLAAPIGPVSIEMIKRGLKDGFWGAFNIRLGGVL
                     GNTLCLIAAYFGLSALQNYGSVFIALGLLGSALLLYMGATTIKKAFKPIHLEFSAKTL
                     RNSKNPLRESLILGFMLAILNPVAVVFWLSIFANDIDPNQPVHWLHLLYNMQIILGVL
                     LWGVLLSTLLEFGRRSLSPKMIRFITFAAGMVLLYFGIKYGYQNILKLQA"
     gene            complement(2063..>2786)
                     /locus_tag="VFP93_01440"
     CDS             complement(2063..>2786)
                     /locus_tag="VFP93_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HET9843305.1"
                     /translation="FVIDIDKTGKNYASNVAKVKKAGIKYVARVVVFPLGGNPSQVKS
                     QQYWANKYQIVQKAINLGADEIQLDYIRYNTKQPPVEKNTQDVFAVLKYFREKTKSQG
                     VPLQIDVFGETSYKPSLRIGQNIKTFAPQIDVLCPMLYPSHFEPYKKHAITPYETVYN
                     SLESIKRQFNQKVPFKIVAYIELSNYRYKMSHAQRIKYIQAQMKAVKDANVHGWYAWS
                     PNNHYDILFEALRGKSLQTAKN"
BASE COUNT          814 a          474 c          547 g          951 t
ORIGIN      
        1 gaggggggat gtctatctaa aaccacaaag cgcttgtaat gaaagcccag tctttttgtt
       61 aactgtgctt cggattccac ccgttgtggc ttcaccatgc gttcttgtac ttgttggatt
      121 tcacctccaa cttttttttc aatttgatat acagtaatgg tatcttgctt ttttagagat
      181 tctaaaagac gcgtttcttc ctgcagcaat tcttgtaatg atagattttg taaggctaag
      241 ttccgataac cgtaccaact aactgcttga ccgtctagaa atccgtgtgc ttcgcctcgc
      301 agatcaatga tccaaacttt ggcgcgatcg tgctttggaa atgcagtatt aagcgttgtt
      361 tctgaaaatt gtccgcttgc agaagcttgg agtttgttta agccttctaa aaaggcgaaa
      421 ggcgtttctt ttttgaccag accaatgggc tcacgccaat tttttggaag ttcggtgtaa
      481 ttttcgttgt caaagactaa taaagtttct gctgtgttcg ccatagtatt ttcaaaacca
      541 aaggtaagaa taagcccaca taaagctaaa caaaacccat tcacactttt ttttacttgc
      601 atcttaaaca tcaattgcag cgttgcatta ttttattata aaaaaaacag gatatttatc
      661 caactgatat ctatttcagt attgagataa aaaatacaga ctgatagtat gaaagataac
      721 taaattacag tgtatcacag cagtatcgag ataggtcatg ggtaaatggt ttgtagtttt
      781 aataagtttt gtaagtttaa atgtgagtgc gcaagaaaca cgattgtatg catgtaagca
      841 gggggatggc actgttctgg tgcaagataa gccaattgat aattgtgctc aagagaaaat
      901 tttccattat gaaacacaag acgctgccaa agaaccacaa ggtttaagag aagcggagat
      961 aaaattattg gacgaaagcc aatcccagca aattttaatc aatcgttacc aaaatgtaga
     1021 tgaaaccgtg ggcaattctt tatcgattag agaaaaagat cgtaggcacg ataattgtta
     1081 tttatatagg agtacgattg aaactgcttt gattaatatc gacgatttag aacataggcc
     1141 ctccgatgat ttgtttgtac gccaatttat tgcgggttta gagcgagaca atgcccaaca
     1201 taagtattat tgtggccaag aatatcccca attgaaagac ttcattcaac ggtataaaag
     1261 ccgctttttt taactatttt ttatctataa ataagtaatt taactttgcg atgatatact
     1321 tcatcgcttt ccgcttgttt gccttttgaa gcctgataca atgctagtta gaaactcaca
     1381 tattctttat tagttatgca cctacaaaaa attttattag gcgttacttt agcggctccc
     1441 atcggtccgg ttagtataga aatgataaaa cgtggcctta aagatgggtt ttggggagca
     1501 tttaatattc gcttaggtgg tgttttgggc aatactttat gtttaattgc cgcctatttc
     1561 ggactatcgg cattacaaaa ttatggtagt gtatttatag ctttaggttt attaggcagt
     1621 gcgctcttgt tatatatggg ggcgactacg attaaaaaag catttaaacc gatacatctc
     1681 gaatttagcg caaagacatt gcgtaattca aagaatcctc ttagagaaag tttaatattg
     1741 ggatttatgt tagccattct caacccggta gcggtggttt tttggctttc catttttgct
     1801 aatgatatcg acccaaatca gcctgtgcat tggttgcatc ttttgtacaa tatgcaaatt
     1861 attttaggtg ttttattgtg gggtgtttta ttaagtacct tgttggaatt tggtcgacgt
     1921 tctttatccc ctaaaatgat ccggtttatc acatttgcag cgggcatggt attgttgtat
     1981 tttgggataa aatatggata tcaaaatatt cttaaattgc aagcataagc aaggtttttc
     2041 taccttgctt atggaaaaag tattaatttt ttgcggtttg caaagatttt cctcttaacg
     2101 cttcaaacaa gatatcgtaa tggttattgg ggctccacgc ataccatccg tgaacgttcg
     2161 catctttaac agctttcatt tgcgcttgaa tatatttaat gcgttgtgca tgagacattt
     2221 tataacgata atttgaaagc tcgatataag caacaatctt aaaaggcact ttttggttaa
     2281 attgacgctt gatagattct aacgaattgt aaaccgtttc ataaggagtt attgcatgct
     2341 ttttgtacgg ttcaaagtga gaagggtaga gcataggaca aagcacatcg atttgtggcg
     2401 caaacgtttt aatattttgt cctatacgca aagagggttt atagcttgtt tcgccaaaaa
     2461 catcgatttg caatggaacg ccttggcttt ttgttttttc tctaaaatat tttaatacgg
     2521 caaaaacatc ctgcgtattt ttttcaacgg ggggctgttt tgtgttatac cggatataat
     2581 ccagctgaat ttcatccgca cctaggttaa tggctttttg aacaatctga tacttgttgg
     2641 cccaatactg ttgggatttt acttgactag gattgccacc taaaggaaag accacaacgc
     2701 gtgcgacata ttttattcct gcttttttta cttttgcgac attggaggca taattttttc
     2761 ctgttttatc gatatcaata acaaaa
//
LOCUS       DASTIB010000084         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_92843, whole genome shotgun sequence.
ACCESSION   DASTIB010000084 DASTIB010000000
VERSION     DASTIB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92843"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFP93_01445"
     CDS             <1..578
                     /locus_tag="VFP93_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843306.1"
                     /translation="VDQGAWQVYVPFSCIPDVQIDPWQKDEAKIFAKRLLNDPKLWSA
                     IDNATGVLYKRDPQSVLVVKTKNGYENLQGENLKTYLETFLKNDLTNANDQPIAFITP
                     KIGKVKILNQTFLSEITKIHASFYGAEKFLLATCNAQDHTSKMVHCVTGVQENIPLVG
                     LYPKKPLCFQNKKDKVLVPEPIQSKTSYVLR"
     gene            complement(563..955)
                     /locus_tag="VFP93_01450"
     CDS             complement(563..955)
                     /locus_tag="VFP93_01450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF015560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HET9843307.1"
                     /translation="MNEIQLKVLEAMSIPIWQHRSQKQAQDAAAILCIFSEKDWSNPE
                     HQTLFRKIIQALQWKDAYYTCVVLSSHQLLDTEKQWILFGEMEHAIVNFQCYTKKWLK
                     VPSLEKMQNAQDAKREAWHLLKPYQRKT"
     gene            complement(952..>2376)
                     /locus_tag="VFP93_01455"
     CDS             complement(952..>2376)
                     /locus_tag="VFP93_01455"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9843308.1"
                     /translation="AKALEKLKVDVIEAGFPISSPGDFKAVHAIAKVIKESSVCGLAR
                     ALSADIDSAAEALKPARLMRIHTFIATSPIHMRHKLQLQPEEVIQRAIKAIQRARLYT
                     HDVEFSTEDAGRSEIDFLCEIIEQAIDAGATTINIPDTVGYTLPDQYGNLIKELRQRI
                     PNSDKAIFSTHCHNDLGLAVANSLAGVLNGARQVECTINGLGERAGNASLEEVVMAVK
                     VRKDRFACDTRLNTQQLVACSKLVSSITGFIVPPNKAIVGINAFAHEAGIHQDGVLKS
                     RETYEIMCAQDVGWTSNRMVLGKHSGRNAFKTRCQDLGMEFNSEEEVNQVFQKFKELA
                     DKKHEIFDEDLLNLFPSRENTIPPLIRLIHLELQSKLHQKPHVNITLDINGEHKQASA
                     SGTGPVDAAFRAIETIMQSGSILQQYSVNAVHPGAEAEGTATIRLEKAGKIVNGKSTN
                     VCLVTASAHAYVNALNLLENKSEL"
BASE COUNT          705 a          485 c          445 g          741 t
ORIGIN      
        1 ctgtggatca aggagcgtgg caagtttatg taccgtttag ttgtattcca gacgttcaaa
       61 tcgatccgtg gcaaaaggat gaagcaaaaa tttttgcaaa gcgtttatta aatgatccaa
      121 aactttggtc tgcaatcgat aatgcaactg gtgtattgta taaaagagat ccgcagtcgg
      181 tacttgtggt aaaaacaaaa aatggctatg agaatttgca aggggaaaat ctaaaaacgt
      241 acttagagac attcctaaaa aatgatctta cgaatgcaaa cgatcaacct attgcattta
      301 ttactcccaa aatagggaaa gtaaaaatat taaaccaaac atttctcagc gaaatcacca
      361 aaatccatgc atctttttat ggtgctgaaa agtttttgct ggcaacatgc aatgcacaag
      421 atcatacctc taagatggtc cattgtgtaa ctggagttca agagaacatt cctcttgtag
      481 gtctttaccc gaaaaaaccc ttatgtttcc aaaacaaaaa ggataaggta ttggtgccag
      541 aacctatcca aagtaagact agttatgttt tacgttgata aggttttaac agatgccaag
      601 cctctcgctt cgcatcttgc gcattttgca ttttttctaa gctaggtact tttaaccatt
      661 tttttgtata acattgaaaa tttacgatgg catgttccat ttcaccaaaa agaatccatt
      721 gcttttcggt atcgagcaat tgatgggaag atagcacgac acacgtatag taagcgtctt
      781 tccattgcaa agcttggata attttacgaa acaacgtttg atgctcagga tttgaccaat
      841 ctttttcgga aaatatacac agaatagcag cagcatcttg cgcttgtttt tgagaacgat
      901 gttgccaaat aggtatagac atcgcctcaa gaacctttaa ttgtatttca ttcatagttc
      961 acttttattt tcaagtaaat ttaaagcgtt aacataagca tgtgcagaag ctgtgaccaa
     1021 gcaaacattc gtgctttttc catttacaat ttttcctgct ttttccaaac gaatagtagc
     1081 tgtcccttca gcctcggcgc ctgggtgtac cgcattaacg gagtattgtt gtaaaatgct
     1141 gccactttgc ataatagttt cgatcgctct aaatgcagca tctacggggc ctgtgccaga
     1201 agcactcgct tgcttgtgct caccgtttat atctaaggta atattaacat gcggtttttg
     1261 atgtaatttc gattgtaatt ctaaatggat taaacgtatt agtggtggaa tcgtattttc
     1321 cctacttggg aataaattta ataaatcttc gtcaaaaatt tcatgttttt tatctgcaag
     1381 ttctttaaat ttttgaaata cttggtttac ttcttcctct gaattgaatt ccatccctaa
     1441 atcttggcaa cgtgttttga aagcatttcg cccagaatgc ttacctaaaa ccatgcgatt
     1501 ggaagtccag cctacatctt gtgcacacat aatttcatag gtttctcgac tttttaacac
     1561 accatcctga tgaatgcctg cttcgtgcgc aaaggcattt attccaacaa tagctttatt
     1621 aggtggcact atgaatcctg ttatggaaga tactaacttt gaacatgcaa ccagttgttg
     1681 cgtatttaat cgggtatcgc atgcaaatcg atctttgcgt accttcactg ccatcacaac
     1741 ttcttctaac gaagcattac ctgcacgctc gcccaagccg ttaatggtac actccacttg
     1801 tcttgcacca ttgagtacgc cagcaagaga attggccact gctaacccta aatcgttatg
     1861 acaatgggtg gaaaaaatgg ctttatcaga attaggaata cgttgtcgca gttctttgat
     1921 gaggttgcca tattgatccg gtaaagtata acctaccgta tcgggaatat taatcgtagt
     1981 agctccagca tctattgctt gttcgataat ttcacataaa aagtcaattt ccgatcgccc
     2041 agcatcttcg gtggaaaatt caacatcatg ggtataaagc ctggcacgtt gaatggcttt
     2101 tattgcacgc tgaatgactt cttctggctg caattgtaat ttatggcgca tatgaatagg
     2161 agatgtagca ataaacgtat gaatacgcat caaacgcgcg ggctttaacg cttcagccgc
     2221 gctatcaatg tccgcggata aggctcgtgc caaaccacaa accgacgatt ccttaattac
     2281 tttagcaatc gcgtgcaccg ctttaaaatc cccaggacta gatatgggaa atcctgcctc
     2341 aataacatct actttgagtt tttcaagtgc tttcgc
//
LOCUS       DASTIB010000085         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_271902, whole genome shotgun sequence.
ACCESSION   DASTIB010000085 DASTIB010000000
VERSION     DASTIB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271902"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFP93_01460"
     CDS             <1..367
                     /locus_tag="VFP93_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9843309.1"
                     /translation="AMENVLLPASKIHKTQEKYEYAKTLLELVGLQGKENRLPRQLSG
                     GEQQRIAIARSLVMKPKYIFADEPTGSLDSVSGDKVMKILEDTNQELGTTIVMVTHEP
                     DYAKLAKRQIHLADGKIVC"
     gene            complement(364..972)
                     /gene="gstA"
                     /locus_tag="VFP93_01465"
     CDS             complement(364..972)
                     /gene="gstA"
                     /locus_tag="VFP93_01465"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HET9843310.1"
                     /translation="MKLYYTPSACSLAVHIILREGNYDFDLESVDLKQKKTETGKDFL
                     TINPLGYVPVLEIDSGQTLTEVAVILQYLGDKASKNLIPSFGSFERYQLLMWLQFIST
                     EIHKAFGPLFKDETPEEFKNTSKQNILKKFEYLDKHLASNDYVFNQQFTIADAYLFVT
                     LRWAFHFKMDLQAYPNLYAYFRRIDKRESVQDTLAAENKKKG"
     gene            1107..1982
                     /locus_tag="VFP93_01470"
     CDS             1107..1982
                     /locus_tag="VFP93_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9843311.1"
                     /translation="MRTAKNVAKKIPREARKQEKQPGLQYKMKPSPIALDPNYKSSDK
                     LKGKVAIVTGGDSGIGQAVALLFAKEGADIVIPYLKEHHDAKNTQKAIEVMGRACMLI
                     AGDLGKERVCQKIAKKAVKAFGQIDILINNAAEQHPTKDFEKITEKQLKHTFASNIFS
                     YFYMIKAVLPHLREGSVIINTSSVTCYKGSDHLMDYAATKGGIVGLTRSLSNALIKKG
                     IRVNGVAPGPVWTPLIVASFSKEHIKKFGKDVPMGRPAQPAEIAPCYLFLACKDSSYM
                     SGQMLHPNGGVVVNG"
     gene            complement(1979..2344)
                     /locus_tag="VFP93_01475"
     CDS             complement(1979..2344)
                     /locus_tag="VFP93_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET9843312.1"
                     /translation="MAKKIADWTGSPYACTLAILLIFVWLFSGPYFQYSDTWQLIINT
                     LTTVITFLMVFLIQNTQNRDTKALHLKLDELIRVHKFAHNSFIDLDELSDEDIRKLEE
                     KYKKLSHKDEEPRDATQKS"
BASE COUNT          766 a          419 c          469 g          695 t
ORIGIN      
        1 tgctatggaa aacgtgttgt tgcctgccag taaaatacat aaaacgcaag aaaaatatga
       61 atatgcgaaa actcttttag aacttgtggg ccttcaagga aaagaaaaca gactgccacg
      121 tcaactctcg ggcggggaac aacaacgcat tgctattgct cgatcgcttg tgatgaaacc
      181 taaatatatt tttgccgatg aacccacagg ctcacttgat tctgtaagtg gcgataaagt
      241 aatgaaaata ttggaggaca ccaatcaaga gttaggtaca accatcgtga tggtaaccca
      301 tgagccagat tatgccaaat tggctaaacg acaaattcat ttagccgatg gtaaaatagt
      361 atgctagccc tttttcttat tctctgctgc taatgtatct tgcacagatt ctcttttatc
      421 gatacgtcga aaataagcat agagatttgg atacgcttgt agatccattt taaaatgaaa
      481 agcccagcgc agggtgacga ataaataagc atctgcgatg gtaaattgct gattaaacac
      541 ataatcgttg ctcgccaaat gcttatctaa atattcaaat ttttttagaa tattttgctt
      601 actcgtattt ttaaactctt ccggtgtttc atccttaaaa agaggcccaa atgctttatg
      661 gatctccgta ctaataaatt gcaaccacat taatagttga taacgctcaa aggatccgaa
      721 actcggaatc aaatttttag atgctttgtc gcctaaatat tgtaaaatga ccgcaacttc
      781 ggttaaggtt tgacccgaat ctatttccaa aaccgggaca taaccgagtg gattaatggt
      841 taaaaaatct ttgccagttt cggttttctt ttgttttaaa tcgaccgatt ctaagtcgaa
      901 gtcgtaattt ccctcacgca aaataatgtg cacggctagc gagcaggcgc ttggtgtgta
      961 atataatttc atgctagtta ctttctaaaa aatagtcttt taataatacc atggcaccaa
     1021 ggtaagtcaa ttaaagtcag aaaataaaca tcttttttca cattatttcc gtctatactt
     1081 tttaaacgtt tgacggagaa aagcgtatgc gcacagcaaa aaacgtggca aaaaaaattc
     1141 cacgtgaagc tagaaagcaa gaaaagcaac caggactgca atataaaatg aagcctagtc
     1201 ctattgcttt agatccaaat tataaatctt ccgataaatt aaaaggaaaa gtagctattg
     1261 ttactggagg tgatagtggc attggccaag ctgttgcgtt gctttttgca aaagaagggg
     1321 cagatattgt tattccctat ctaaaagaac atcatgatgc aaaaaatacg caaaaagcta
     1381 tcgaagtgat gggtcgtgcg tgcatgttaa tagcagggga tttaggtaaa gagagggttt
     1441 gccaaaagat cgctaaaaaa gctgtgaaag catttggtca aatcgatata ttaatcaaca
     1501 atgcagcaga gcaacatccg acgaaagatt ttgaaaaaat taccgagaag caattaaaac
     1561 acactttcgc aagcaatatt ttttcctatt tttatatgat taaagcggta ttgccgcatc
     1621 ttagggaagg cagtgttatt attaatacgt cttcggttac gtgttacaaa ggcagtgatc
     1681 atttaatgga ttatgctgct accaaaggtg ggattgtagg attaacgcgt tctttatcaa
     1741 acgcgctcat aaaaaaagga attcgagtca atggtgtagc cccgggacct gtgtggactc
     1801 cgctcattgt ggcaagtttt agcaaagaac atattaaaaa atttggaaaa gatgtaccca
     1861 tggggcgacc tgctcagccg gcagaaattg caccctgtta tttatttttg gcttgcaagg
     1921 actcttctta tatgtccggc caaatgctgc atccgaatgg gggcgttgtg gtaaacggtt
     1981 aacttttttg cgtagcatct cgcggttctt catctttatg actaagtttt ttgtattttt
     2041 cctcaagttt gcgtatatct tcatcactta actcatctaa atcgataaag gaattgtggg
     2101 cgaatttatg cacgcgaatc aattcatcga gttttaagtg cagcgctttt gtgtcacggt
     2161 tctgcgtatt ctgaattaaa aataccatta agaaggtaat gaccgtagtc aaggtattaa
     2221 taataagttg ccaagtatcg gaatattgaa aataaggtcc actaaaaagc catacaaaaa
     2281 taagaagtat tgctaaagtg caagcataag gtgagccagt ccaatcggct attttttttg
     2341 caataaaat
//
LOCUS       DASTIB010000086         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_614599, whole genome shotgun sequence.
ACCESSION   DASTIB010000086 DASTIB010000000
VERSION     DASTIB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614599"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..319
                     /locus_tag="VFP93_01480"
     CDS             149..319
                     /locus_tag="VFP93_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843313.1"
                     /translation="MKILSEIEIEQVQGAGAGPEIFEGLATAASGLLILAVMGAFVIG
                     ACVGGIFYSLKS"
     gene            complement(585..770)
                     /locus_tag="VFP93_01485"
     CDS             complement(585..770)
                     /locus_tag="VFP93_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843314.1"
                     /translation="MRLCIKSWMAAFAAMTPSSLKPRGRLNIFFTDISECYKELISYL
                     WIALNEETIKLEDGFTV"
     gene            complement(796..>2238)
                     /locus_tag="VFP93_01490"
     CDS             complement(796..>2238)
                     /locus_tag="VFP93_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843315.1"
                     /translation="MQLAVQQGHLDALNSLLSYNNVATLATFNNNQILTEAQMRVNGD
                     NEDRARYQPIIDRLMQVEAVANLVNGQAQQPVTTLANITQFAENSMQSLNAQERNIVS
                     HLKSHYKSIYNEKNFEVIRAEILSYLEESYKKSPACDKGSPLPLEYTANLSNSALESY
                     YKHLNHGAWRYLSDSNPWMAADAKYIQILEDGRKVAIISDADKELLSYIWIALNDETV
                     KLEEGFTVEGNKNVFVQNLGELNRAHNHDERAENSEEQQAYLDKVPGIKTTRADDLEA
                     DKPTCVYGVTKRLLESPYGHPHIKPLDPKLMQNWMEALLFGRKEKAEDPDNIADRLDK
                     LSLHELKNIQENLMDAMSGEEFVCHPALQIPQQQLDAFIEKVKTWYGADEITQERNPK
                     LVLNPAGSGFIAEKHISYVALIQFCANDAMNCFAQGLNTYLTARIQSLEELEKSDVEL
                     KNVNSFTPSYDASKSDSDADPETKVQNMRP"
BASE COUNT          638 a          424 c          409 g          767 t
ORIGIN      
        1 ggatggcgtg ttagagagtg ttgtcactaa agcgtaatag ctttacccta tttcagttag
       61 cattacgaat ttttatgttg aatattgact aaatttaatt ggtatgtgaa catttactcg
      121 cttatagcat ctaactaaga gtatatctat gaaaatttta tccgaaattg aaattgaaca
      181 agtacaggga gcaggagcag gacctgaaat atttgaaggt ttagctacag cagcctcagg
      241 attgttaatc ttagctgtga tgggagcctt tgtgattgga gcgtgcgttg ggggaatttt
      301 ttattctctt aaatcttaat atcaaaaata catttattgg atcttttttt ataagcttag
      361 gatctaaagg tttgatatgg ggatgaccgt aagggctctc gagtaagcgt ttggtaacac
      421 ccttacacat gtgggtttat aggccaaaaa tcatctactc gcgttgtttt gatatcggga
      481 actttatcta aaaataggct tgtttttcta aaggatcctc ggcacgttca tcatgattat
      541 gggcgcgatt taattcacta agttttacgc aaatacattg ttacctacac cgtaaaacca
      601 tcttccagct ttatggtctc ttcgttaagc gctatccaga gatagcttat taattcttta
      661 taacactctg aaatatctgt gaaaaatata tttaaacggc cgcgaggttt taaactggaa
      721 ggtgtcatgg cagcaaaagc tgccatccaa ctctttatgc aaagacgcat agcaatcatg
      781 caaaaaaagc aatcactagg gacgcatgtt ttgaactttt gtttctggat ctgcatcgct
      841 gtcgctttta ctcgcatcgt aactaggtgt gaaactattt acatttttca attctacatc
      901 ggatttttcc aattcttcta atgattgaat acgcgctgtt aaataagtat ttaatccttg
      961 tgcaaaacaa ttcattgcat cgttcgcaca aaattgaata agcgcaacat agctaatatg
     1021 cttttctgcg ataaaaccag aacctgcagg atttaatact aatttaggat ttctttcttg
     1081 cgtaatttca tccgcgccat accaagtttt gactttttca ataaatgcgt caagctgttg
     1141 ttgtggaatt tgaagagccg ggtggcatac aaattcttct ccactcattg catccataag
     1201 attttcttga atatttttta attcatgcaa agataactta tcaagcctgt ctgcgatatt
     1261 atctgggtct tctgcttttt cctttcttcc aaaaagcaat gcttccatcc aattttgcat
     1321 aagcttagga tctaacggct taatgtgtgg atgaccatac ggactttcca ataatcgttt
     1381 ggtaacacca tatacgcacg tcggtttatc ggcctcaaga tcatctgctc tcgttgtttt
     1441 aatcccagga actttatcta gataggcttg ttgttcttca gaattctcgg cacgctcatc
     1501 atgattatgg gcgcgattta attcacctaa gttttgtaca aatacatttt tgttgccttc
     1561 taccgtaaag ccctcttcta acttcaccgt ttcatcatta agagcaatcc atatatagct
     1621 cagcaattct ttatcggcat cagaaataat ggcaactttt ctaccatctt ctaaaatttg
     1681 aatatactta gcatctgctg ccatccacgg attactatca cttaaataac gccaggcacc
     1741 atggtttaaa tgtttataat aagattccaa tgcactatta cttaaattgg ctgtgtattc
     1801 taatggaagt ggactccctt tatcgcaagc aggagatttc ttataagatt cttcaagata
     1861 acttaaaatt tcagcgcgaa taacttcgaa gtttttttca ttataaatgg atttgtaatg
     1921 gctttttaaa tgcgaaacaa tgttgcgttc ttgtgcattt aacgattgca tcgaattttc
     1981 ggcaaattgc gtgatattgg ctaaggtagt aacaggttgc tgtgcttgtc catttacaag
     2041 gttagccaca gcttcaactt gcattaatct atcgataatg ggttgatagc gagctctatc
     2101 ctcattatca ccattcaccc gcatttgcgc ctcagtaagg atttgattgt tgttaaaagt
     2161 cgcaagcgta gctacgttat tgtaggataa taagctatta agcgcatcta agtgaccctg
     2221 ttgaacggca agttgcat
//
LOCUS       DASTIB010000087         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_827214, whole genome shotgun sequence.
ACCESSION   DASTIB010000087 DASTIB010000000
VERSION     DASTIB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827214"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFP93_01495"
     CDS             <1..877
                     /locus_tag="VFP93_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9843316.1"
                     /translation="VIMMAATNRLESLDTALLRPGRFDRSIYIRLPNFNERVALFKVH
                     LKQVPHTNIDLESLAGSTYGFSGADIKNLVNEAALHAVKAKRKAVDKSDFFYAMDKIK
                     LGLEVKSAIVSEEEKLLTAYHEAGHTIVGIFSPNYNYEFNKVTIGVRDETLGVTHFES
                     IGDNYGYSKAFIEDNIAMLLGGRLAEEMLVGKEHITTGASNDLKIATELAEKMVVDWG
                     FSDLGNNISFAVLDNYSSDLIHQEIKKILDAAQTKARSILESKRDKLDELAKALVKKE
                     ILDRSEVMKILEVNQ"
     gene            992..2041
                     /locus_tag="VFP93_01500"
     CDS             992..2041
                     /locus_tag="VFP93_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9843317.1"
                     /translation="MKKIIVFSLFTFFALVMGIFLWVQFHKGEPKLQDFKVQVKSYEV
                     GSEIIHDKVEALGTTFANESVNITPNVSELIAELKFEEGDLVKKGEIIVILDQAEEQA
                     QKEAQIAQVNEHKRELKRLENLLKVNATAQNAFDERKTKLAIAQYFIEETNARIEDRT
                     IRAPFDGILGLRKVSVGSLVEPGTLITTIDDLSRIKLDFTVPTTFLSVLKEGTQILAT
                     STSYDETFKGTVSNIDTRADPITRAVLIRAILPNPELKIKPGILMTVVLLKNEREAIV
                     VPEESILQIQDDHFVFVIENPKEPSVHQKRVEIGLRMPGKVEILSGLKQKDLIVRDGI
                     SKVRDGQKIIIQPET"
     gene            2050..>2335
                     /locus_tag="VFP93_01505"
     CDS             2050..>2335
                     /locus_tag="VFP93_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9843318.1"
                     /translation="MFLSEVSVKRPVLAAVMSLLLIAFGLVSFFKLPLREYPNIDPPV
                     VSIQTVYPGAAANIVETRITEIIEDRVAGVEGIRTITSSSQDGISDISIEF"
BASE COUNT          785 a          371 c          482 g          697 t
ORIGIN      
        1 tgttattatg atggctgcaa caaatcgctt agaatcttta gataccgcgt tgttgcgccc
       61 cggaagattt gatagaagta tctacatacg ccttcccaat tttaatgaac gtgtcgcatt
      121 attcaaagta cacctcaagc aagtacctca taccaacatc gatttagaat cgttagcagg
      181 ttctacgtat ggattttctg gggcagatat taaaaatctt gtgaacgagg cagctttaca
      241 tgcagtaaaa gcaaagagaa aggcggtgga taaatcggat ttcttttatg ccatggataa
      301 aattaaatta ggcctagaag taaaatcagc gattgtaagc gaagaagaaa agttattaac
      361 agcttaccac gaagccggtc acacgattgt tggaattttt tcacccaact acaattatga
      421 atttaataaa gtaaccattg gtgtgcgcga tgaaacatta ggtgttacgc actttgaaag
      481 tattggtgat aactatggct attcaaaagc ttttattgaa gataacatcg cgatgctttt
      541 agggggacgt ctcgccgaag aaatgcttgt cgggaaagag catattacta ccggtgcttc
      601 caatgatcta aaaatagcca ctgaattagc agaaaaaatg gtcgtagact ggggtttttc
      661 tgatttagga aataatattt cttttgcagt tttagataat tattcttctg atctcattca
      721 tcaagaaatc aaaaaaatat tagatgcagc ccaaaccaaa gctagaagta ttttggaaag
      781 caaaagagat aaattagatg aactggccaa agcgttagtt aaaaaggaaa tattggatcg
      841 ttcggaagta atgaaaattt tagaagtcaa tcaataataa attattatta ttctataata
      901 acatacaagt aagttgttaa acataatacc tatgatgcta aatagaaaga gagcacctgc
      961 tttttatagc tagtattcta aaagggtatt catgaaaaaa attattgtct tttctctttt
     1021 tacatttttc gccttggtta tgggtatatt tttatgggtg caatttcata aaggcgagcc
     1081 aaaactgcaa gattttaaag ttcaagtaaa aagctatgaa gtcggctctg agattattca
     1141 cgacaaggta gaggctttag gaactacgtt tgccaatgaa tctgtcaata taacaccaaa
     1201 cgtttctgaa cttatagcgg aattaaagtt tgaagaaggc gatttggtaa aaaagggtga
     1261 aatcattgtt attttggatc aagcagaaga acaggcacaa aaagaagcac aaattgctca
     1321 ggtaaacgaa cataaacgtg aactaaaacg tttagaaaat ttgctcaaag tgaatgctac
     1381 tgctcagaat gcctttgatg aaaggaaaac gaagttagcg attgcacaat attttattga
     1441 agaaacgaat gctcggattg aagataggac tattcgagcg ccatttgatg gtatcttggg
     1501 gttacggaaa gtaagtgttg gttctttagt tgagcctggt acactcatta ccactattga
     1561 tgatttgagc agaattaaac tagatttcac agttccaacg acgtttttat ctgtactaaa
     1621 ggaaggtact cagattctcg cgacaagtac cagttatgat gaaacattta agggaaccgt
     1681 aagtaatatt gatacgcgtg ctgatccgat aacacgcgca gttttgatta gagctatttt
     1741 gccaaatcct gaattaaaga ttaagccagg aattctaatg acggttgttc ttttaaaaaa
     1801 tgaacgtgaa gctattgtgg tacccgagga atcgatctta caaatccaag atgatcattt
     1861 cgtattcgtt attgagaatc caaaagaacc atcagttcac caaaagcgag ttgaaatcgg
     1921 tttaagaatg cctggcaaag ttgagatttt atccggttta aagcaaaaag atttaattgt
     1981 tcgagacggt atcagcaagg tgcgagacgg tcaaaaaatt atcattcaac ctgaaaccta
     2041 gggaaaggta tgtttctttc agaagtgtcc gtaaagaggc ctgttttagc agctgttatg
     2101 agtttgctac ttattgcttt tgggttagtg tcttttttta aattaccttt gcgcgaatat
     2161 ccaaatattg atccacccgt tgtttctata caaacggtat acccaggtgc agccgcaaat
     2221 attgttgaaa cacgaatcac cgagattatt gaggatcggg ttgccggtgt agaagggatt
     2281 cgaacgatta cgagctcaag tcaagatggc atatcagata tctccattga atttt
//
LOCUS       DASTIB010000088         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1046727, whole genome shotgun sequence.
ACCESSION   DASTIB010000088 DASTIB010000000
VERSION     DASTIB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046727"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="hslU"
                     /locus_tag="VFP93_01510"
     CDS             <1..857
                     /gene="hslU"
                     /locus_tag="VFP93_01510"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET9843319.1"
                     /translation="REKLNNGEIEDQEIELEVAQSSIGVEIMAPPGMEDMTNQLQSMF
                     QNISGGKTKKKKMRIKEAKKILQEEEAAKLVNDDEIRAKACDRAEQHGIVFIDEIDKV
                     TQRGDMHGPGVSREGVQRDLLPLVEGCTVSTKYGIVRTDHILFIASGAFHLSKPSDLI
                     PELQGRLPIRVELTALNAEDFEKILTEPNASLTEQYTALLSTEGVTVRFSASGIKRIA
                     EIAFEVNERTENIGARRLHTVVERLLENISFEANDLSGQTLEIDSMYVDKQLKDLVQD
                     QDLSRFIL"
     gene            854..1597
                     /gene="ubiE"
                     /locus_tag="VFP93_01515"
     CDS             854..1597
                     /gene="ubiE"
                     /locus_tag="VFP93_01515"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET9843320.1"
                     /translation="MTEKNTDFGFQYVPLSKKQVKVNEVFNSVAQHYDLMNDLMSFGL
                     HRIWKRQAIMLARISSNHTVLDLAAGTGDLTQLILKKKASVVCADINYTMLTLARDKL
                     IDAGLVQGAHLATLDAQSLPFAPGTFDRIIIGFGLRNVPDKEKALKEMFRTLKDHGIL
                     IILEFSHLQEPMLAKLYDHYSFKVLPWLGRKVAKDSTSYQYLAESIRKHPDQEHLKTA
                     LMAAGFNKCDYHNILFGVVAIHCAYKSTL"
     gene            1594..>2126
                     /locus_tag="VFP93_01520"
     CDS             1594..>2126
                     /locus_tag="VFP93_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9843321.1"
                     /translation="MKNYALLLLNVLLEQLQKIDPEAFAILNRHEGKVIEITLEDIPL
                     TLYMEVQNNNLAILPVQSERANLKITAPLGSFIKLFFTKNPTMANLVENHVVVLGDTG
                     LLHALQNFLQNFNLDIEHFMALSMGEIPATQLTAFFQSTFQRMQDTLKNQTQNLSDYF
                     TYEKKFLLSLNRFSEFS"
BASE COUNT          699 a          392 c          413 g          622 t
ORIGIN      
        1 ttcgagaaaa attaaataat ggggaaattg aagatcaaga aatagaactg gaagttgccc
       61 aatcgagtat tggtgtagaa attatggccc ctcctggcat ggaagatatg acgaaccaat
      121 tgcaaagcat gtttcaaaat atctcgggtg gcaaaaccaa aaagaaaaaa atgcgcatta
      181 aagaggcaaa gaaaattctt caagaagaag aagctgccaa actcgtcaac gacgatgaaa
      241 tccgtgcaaa agcctgcgat cgagctgagc aacacggtat tgtttttatc gatgaaattg
      301 ataaagtcac gcaacgtggt gatatgcatg gtcccggtgt ttctcgagaa ggtgtgcaac
      361 gcgatttatt acctttagtt gaaggttgca ccgtgtcgac taagtacggg attgttagaa
      421 ccgatcatat tttatttatt gcaagtggcg ctttccatct ttctaaacct tcggatctta
      481 tcccagaact tcaagggcga ttacctattc gggtggaatt aactgcttta aacgccgaag
      541 atttcgaaaa aattctcacg gaacccaacg cctcgttaac tgagcaatat acggctcttt
      601 taagcacaga aggtgtaacg gttcgtttta gtgcgtctgg aattaagcgt attgcagaaa
      661 ttgcatttga agtgaatgaa cgcacagaaa acattggtgc tagaaggctt cacactgtgg
      721 ttgaaaggct cttagaaaat atttcgtttg aggccaacga tctaagtgga caaacgctag
      781 aaatagactc gatgtatgtg gataaacaac taaaagattt agttcaggat caagacttaa
      841 gtcgctttat tttatgaccg aaaaaaatac agactttggc tttcaatacg ttcctctttc
      901 aaagaagcaa gtgaaagtca atgaagtatt taatagtgtt gcacaacatt atgatttgat
      961 gaatgactta atgtcatttg ggctacatag aatttggaaa cgtcaagcta ttatgctagc
     1021 ccgtatttct tccaatcata cggtacttga tttggccgct ggaactggag atttaacgca
     1081 gttaattcta aaaaaaaagg catcggtagt ttgtgcggat atcaactaca cgatgttaac
     1141 cttggctaga gacaaattaa tcgatgcagg tttggtgcaa ggcgcccatc tagcaacgct
     1201 tgatgcacaa agcctacctt tcgcaccagg aacttttgat cgaattatca tcggatttgg
     1261 tttacgtaat gtacctgata aggaaaaagc cttaaaggaa atgtttcgta ctttaaaaga
     1321 tcacggtatt ctcattatct tagaattttc acatcttcaa gaaccgatgc ttgctaagct
     1381 ttatgatcac tattcgttta aagttttacc gtggctagga cgtaaagtgg ctaaagattc
     1441 gacaagctat caatatttag cagaaagtat ccgaaaacac cccgatcaag aacaccttaa
     1501 aaccgcactt atggctgcag gttttaataa atgtgattat cataatattt tatttggcgt
     1561 agtggctatt cactgtgcct acaaatcaac gctatgaaaa actacgcatt acttttacta
     1621 aatgtcttgt tagagcaatt acaaaaaata gatcccgaag cttttgccat cttaaataga
     1681 catgaaggca aagtgatcga aattacgtta gaagatattc cgctgacttt gtatatggaa
     1741 gtgcaaaaca ataatttggc tatcctacct gtacagtctg aacgcgcaaa tttgaaaatt
     1801 accgcgcctt tgggttcttt tataaaactt ttttttacta aaaatcctac catggcaaat
     1861 ctggttgaaa atcacgttgt ggttttgggc gataccggtt tattacatgc attacaaaac
     1921 ttcctacaaa attttaattt agatatagaa cattttatgg ctttaagtat gggtgagatc
     1981 cctgcaaccc aacttaccgc attttttcaa tccaccttcc aacgcatgca agatacgcta
     2041 aaaaatcaaa cacaaaactt aagcgattat ttcacctatg aaaaaaaatt cttattatcg
     2101 ctcaaccgtt tttcagaatt tagcaa
//
LOCUS       DASTIB010000089         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_882519, whole genome shotgun sequence.
ACCESSION   DASTIB010000089 DASTIB010000000
VERSION     DASTIB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882519"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFP93_01525"
     CDS             <1..570
                     /locus_tag="VFP93_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET9843322.1"
                     /translation="VKFPQGIHEDMFDNFFKNCDLFIDGLDFFAIHVRSKAFQYCYEH
                     NIPAITAAPLGLGASMLSFLPNQQSFEQYFRLNNQPPQEQILRFLTGLAPSSLHRKAL
                     VDPSRVQLAAQKAPSLTIGCELSAALCASQSFKILLKRGLVISAPKSLHFDAYTYQMR
                     VSWRPLGAYNPLQIWQRFWVRKAFTTSNR"
     gene            572..1315
                     /locus_tag="VFP93_01530"
     CDS             572..1315
                     /locus_tag="VFP93_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HET9843323.1"
                     /translation="MQPILDIFKTYFEIHIANTSQQLEKIFRLRYDVFCTEFQFEKEE
                     DCPNALEQDLYDPFSIEALLYHPNDGKIAGCVRLILPVNTCPPLPVENFCHQGLCPEQ
                     KNAYAEVSRLTIAKDFRRRLWDGHLPSGVSDNPAQIGVIGRNFPLPAVSMMFTGASLC
                     RLLLLDYVYAMMEPKLAHAMTAYGIEFQQIGDVHDYHGQRAPYRLDPLLIWETVQPEL
                     YPLLNFIYFKVSQREAIPVSAQYKKIQTG"
     gene            1318..2133
                     /locus_tag="VFP93_01535"
     CDS             1318..2133
                     /locus_tag="VFP93_01535"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9843324.1"
                     /translation="MAKPRWMYTIAHYFGLLCVAYISLSILFFLLSDSVLFQPPYPTQ
                     FQLSNSTLIIPSGNKKIIATYLPSRSHYTILYSHGNAEDLATLGPLLHYFQDQGFSIL
                     SYDYQGYGASDGKTSEQNTYQDIMAVYQYMIRTLKIPAQNIVLYGRSLGSGPSLYLAN
                     HKPVAGIILEAPFVSAYRTLTYYAIFPFDKYNNVAWIKNLQVPILFIHGEEDSVIKSW
                     NTKQLAQFVRGSKYFYWVKNANHNDLLIIAGERYWVELQKFIDIIAQSQQQKH"
     gene            2272..3696
                     /locus_tag="VFP93_01540"
     CDS             2272..3696
                     /locus_tag="VFP93_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET9843325.1"
                     /translation="MLYSMLTLAATCTGPYRGQTLNRAEILTILQEAKKTDTINLCGT
                     LLSNINLSKVNLHGVDLSYASLQNAQLEQADLSRAILVKTNLSGANLQSSFLQAADLT
                     QANLTRAQLQNSIFHNTVLIKANLTHAILEGIQLQYANVDSANLAHVQGKEANFNFAK
                     ISNANFYHSQLNDAQFEHSVAIFTNFNKASLKKTNFKKSNLQHATFIGADLTNALMQD
                     TDISQVNFHQANLREMEYYPTLDGLPNLDALSKSKNFATMHFSEKGFGPLSLLKLQLA
                     YQKKGLRAPERLITSMLKQHEMQRNFNQGGFHTLLGILQYIFFYLPCEFGLDPMRPLW
                     IGFAFFMLATIPYYLSLGSQSPYAGILIQWPSKRDHNYLLEASYQHFVKIYPTDPWII
                     ASIKRYRMVLTALYLSLLSSLKIGFDDFNLAHWITYMQSREYNLIPRGWVRSLAGFQA
                     IFCAYLLLLWAITLFGRPFEVNLS"
     gene            3761..>3988
                     /locus_tag="VFP93_01545"
     CDS             3761..>3988
                     /locus_tag="VFP93_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9843326.1"
                     /translation="MRDGTATRQKLERCALDLFVRQGINATTIKDIANAAQIAEGTLY
                     RHYTSKDDLAAALYLKAYKEFSVLLQNVIEQN"
BASE COUNT         1216 a          822 c          729 g         1221 t
ORIGIN      
        1 gtgaagtttc ctcaagggat ccacgaggat atgttcgaca atttttttaa aaattgtgat
       61 ctttttatcg atggtttaga cttttttgct atccatgttc gaagcaaagc gtttcaatat
      121 tgttatgaac ataatattcc tgcgataacc gcagcgccgc taggtttagg ggcttccatg
      181 ctttctttct taccgaatca gcaatccttc gaacagtatt ttcggttgaa taatcaacca
      241 ccccaagaac aaattctgcg ttttttaaca gggttagcac cctccagcct gcatagaaaa
      301 gcgttagtag atccaagccg tgtccaatta gccgcacaaa aagcaccttc tttaaccata
      361 ggttgtgaat taagtgcagc gctctgtgcg agtcaatcgt tcaaaatatt gctaaaacgt
      421 ggtttagtga taagcgcccc gaaatcactc cacttcgacg cctatactta tcaaatgaga
      481 gtatcttgga ggcccttggg cgcttataat cctttacaga tctggcaaag attttgggta
      541 aggaaagcgt tcaccacctc aaacaggtag tatgcaacca atacttgaca tttttaaaac
      601 ctattttgaa atccacattg ccaatactag tcagcaattg gaaaaaatat ttcgcctgcg
      661 ttatgacgtt ttttgtacgg agtttcaatt tgagaaagaa gaagattgtc cgaatgcctt
      721 agagcaagac ctatacgatc ccttttctat tgaagccctc ttataccatc ccaacgacgg
      781 aaaaatcgcc ggctgtgtac gacttatttt accagtcaac acatgcccac ctttacctgt
      841 ggaaaatttt tgtcatcaag gcttgtgtcc agaacaaaaa aacgcttatg cggaagtgtc
      901 tcgacttacc attgcaaaag attttaggcg ccgattatgg gacggtcatc taccctcagg
      961 cgtaagcgat aaccctgcgc aaataggggt tattggtcgc aactttccct tacctgccgt
     1021 cagtatgatg tttaccggag cgtccttgtg tcgcctttta ttgttagatt atgtatacgc
     1081 aatgatggaa cccaaattgg cgcatgcaat gactgcctat ggtatcgaat tccaacagat
     1141 aggagacgtg catgattatc acggccagcg cgcaccctat cgtttagatc cgttattgat
     1201 ctgggaaacg gtccaacccg aattatatcc cttattaaat tttatttatt ttaaagtatc
     1261 gcaaagagaa gccatacccg tatccgcgca gtataaaaaa atacaaactg gctaaccatg
     1321 gcaaagcccc gttggatgta taccatagcg cactattttg gtcttttgtg tgtagcgtat
     1381 ataagcttaa gtattttatt ttttttgttg tcggattcgg ttttatttca gcccccgtat
     1441 cctacgcaat ttcaattgag taattctacg ctaatcatac ccagtggaaa caaaaaaatc
     1501 atcgccacct atctacccag tcgatcgcac tatacgatat tgtatagtca cggtaacgca
     1561 gaggatttag cgactttagg gcctttattg cattattttc aagatcaagg tttttcgatt
     1621 ctttcttatg attatcaagg ctatggcgca agcgatggaa aaacctcaga acagaatacg
     1681 tatcaggata ttatggcggt ttatcagtac atgattcgta ccttgaaaat tcctgcgcag
     1741 aacattgtac tttacggacg ttcgttaggc tcaggaccaa gtctctattt agcaaaccat
     1801 aaacccgtag cagggatcat tttagaagcc ccttttgttt cggcttacag aacccttacc
     1861 tattacgcta ttttcccttt tgataaatat aacaacgtcg cttggatcaa aaaccttcaa
     1921 gtgcctattt tgtttattca tggggaagaa gactccgtga taaaatcctg gaatactaag
     1981 cagttagccc aatttgtccg aggatcaaaa tatttttatt gggtcaaaaa tgccaatcac
     2041 aatgatctat taataatcgc aggtgaaagg tattgggttg aattacaaaa atttatagac
     2101 ataatcgcac aaagccaaca gcaaaagcat taaaaattgc ccaatcgatc ttttatcttc
     2161 acaagggctt ttgaagttgt gcactgcatt gaagatgaat agcctacact atttatataa
     2221 aatctaacgt acttgctcta ctttatgaaa tttttatttt tggttgtatg catgctttac
     2281 tccatgctta ctttggcggc cacctgtaca ggcccgtatc gtggacaaac cttaaaccgg
     2341 gcggaaattc ttactatttt gcaagaagca aaaaaaaccg ataccattaa tttgtgtggt
     2401 accctgcttt cgaatattaa tttatccaaa gtcaatttac atggtgtcga tctttcttat
     2461 gcttcgttac aaaatgccca attagaacaa gcggatctta gtagagctat tttggtaaaa
     2521 accaatttat cgggtgcaaa tttacaatca tcttttttgc aggcagcaga tttaacgcaa
     2581 gccaatttaa cacgcgcaca attacaaaac agtatttttc acaacaccgt attaattaaa
     2641 gccaatttaa cacacgctat attagaagga attcaattgc agtatgccaa tgtagactct
     2701 gcaaatttag cgcacgttca aggaaaagaa gccaatttta attttgcaaa aatctccaac
     2761 gcaaattttt accacagcca attgaatgat gcacagtttg agcacagtgt tgcaattttt
     2821 acaaatttta ataaagcttc cttaaaaaaa accaatttta agaaaagtaa tcttcagcat
     2881 gccaccttta ttggagcaga tttaacgaac gcgctgatgc aagataccga tatttcgcag
     2941 gtaaactttc atcaagctaa tttacgagaa atggaatatt atccgacttt agacggctta
     3001 cccaatcttg acgctctatc aaaaagtaag aattttgcga ctatgcattt ctctgaaaaa
     3061 ggctttgggc ctttaagctt attaaagctt caacttgcgt accaaaaaaa aggattacgc
     3121 gctccagaac gccttataac gagtatgtta aagcaacatg agatgcaacg aaatttcaac
     3181 caaggtggat ttcatacatt attaggtata ctacaatata tattcttcta tttgccgtgt
     3241 gaatttggct tggatccgat gcgtcctttg tggataggct ttgctttttt tatgcttgcc
     3301 accattcctt attatttatc tttagggtcg caatcgccct atgcgggtat tctcatacaa
     3361 tggccaagta aacgagatca taattatctc ttagaagctt cttaccaaca ttttgtcaaa
     3421 atttatccga ccgatccttg gatcatagcg tctataaaaa gatacagaat ggtacttact
     3481 gcactctact taagtttact aagttcgcta aaaataggct tcgatgattt taacctagcg
     3541 cattggataa cctatatgca atccagagaa tataacttaa taccacgcgg ctgggtccgg
     3601 tcgctggcag gttttcaagc gatcttttgc gcttatttac ttttattatg ggcgataaca
     3661 ctctttggtc gaccttttga agtaaacttg tcctagttgg ttgacaaaaa ggttgtgctt
     3721 agtcacaatt tgctcactat taaatgaggt aatattaaac atgcgtgatg gaactgcaac
     3781 ccgtcaaaaa ctagaacgat gtgcacttga tttattcgtg agacaaggaa ttaatgcaac
     3841 aaccatcaaa gatatcgcaa atgccgcgca aatagcagaa ggtacattat atcgtcatta
     3901 taccagcaaa gacgatttag cggcggctct ttacctaaaa gcttacaaag aattttcggt
     3961 actgttacaa aatgttatag aacagaac
//
LOCUS       DASTIB010000090         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1295679, whole genome shotgun sequence.
ACCESSION   DASTIB010000090 DASTIB010000000
VERSION     DASTIB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295679"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFP93_01550"
     CDS             <1..1118
                     /locus_tag="VFP93_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HET9843327.1"
                     /translation="YGSVTFQPPTFVPPDASAYPGSWFLQVESLSNAYIKMSDLTSTT
                     APGSSLIQRPNMISGEAKQLVDPAVKNLSALARAFIGHLSERDVDPIASFQKTGSEIM
                     VTTEALFFTLVVIAVLAMSIACIASGAQPGCYIVGIIITLFMPVLMIMLGLLWTAGVS
                     LGLYLPLVPYLVFTFTALGWLLICIEAMVAAPIVALGITTPGQDLLGRASPAVMLITN
                     IFLRPSLMVMGFVAAARLAKPVINMINFGFEGVVLMQIGALLLFGNIALIVIYAGICV
                     TAIHQVFSLIYILPDRVIRWIGGQAEQSMVERAMEETKKAAETATHATQQVMKGTTEG
                     IVSAGKMAVEKGKAPAEGGGGGGGGQGGAQGVSGLQM"
     gene            1220..2332
                     /locus_tag="VFP93_01555"
     CDS             1220..2332
                     /locus_tag="VFP93_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843328.1"
                     /translation="MANSVPQLNVGKSREITNSKKNQRTPSFKEQEQVREELKKRLKK
                     YAETTPSMVASNGQSFGDSIIHMNKFNQEAIAVQMLIEDAIFIMQLLFALCGAKLVMI
                     DAQEWNQARQKDLALRENLRAKKENVQNEIAEIEKTLAEKKLETEEKDLKLKSNQEEL
                     NNIKKQRVALRDPNNGAITDAAKEAELVQKIKDLLEVQKPYTEYFEEVKKLEDRKKSL
                     QDELKHVNEQQETTYDKTEYPYPTGNDAESKRIRLDTERRLAERKIYPIKWMKTPTGP
                     KYEFETENGPIVNPTAITYDIAVANGLTPNPEYGVSIRKVVIDNMVKFWGMAPDWLYY
                     IEHQNRPELRQEKAQNDKDDPILARNNMPAPKPPGR"
BASE COUNT          766 a          447 c          487 g          644 t
ORIGIN      
        1 actacggttc ggttacgttt caaccaccta cttttgtgcc ccccgatgct tctgcttatc
       61 caggtagttg gtttttacaa gtagaaagct tgagtaatgc ttacattaaa atgagcgatt
      121 taacctcaac aacagcacca ggttcttcat taattcaacg tcctaatatg ataagtggtg
      181 aagcaaagca attggtcgat cctgccgtaa aaaatttaag tgcattggca cgcgccttta
      241 tcggtcatct ttcggaacgt gatgttgatc ctatcgcctc ttttcaaaaa accggctcgg
      301 aaataatggt caccacagaa gcgttgttct tcactttagt ggtgatcgca gtccttgcta
      361 tgtcgattgc ttgtatagct tcgggtgctc aaccgggttg ttatatcgtt ggtattatca
      421 tcactttatt tatgcctgtt ttaatgatta tgctaggact tctttggact gctggcgtat
      481 ctcttggatt atatttaccg ctcgttcctt atcttgtctt tacgtttacc gcattgggtt
      541 ggctgttaat ttgtattgaa gcaatggttg cagcccctat tgttgcttta ggtattacaa
      601 ccccgggaca agatttactt ggaagagcaa gtccagcggt tatgcttata acaaacatct
      661 ttttgcgccc atcgctcatg gtaatgggat ttgtcgcagc cgctcgactg gcaaagcctg
      721 ttatcaatat gattaatttt ggtttcgaag gtgtagtgct catgcaaatc ggtgctttat
      781 tactttttgg taatattgca ctcattgtga tatatgcagg tatttgtgtt actgccattc
      841 atcaagtatt ttcccttatt tacattctac ccgatcgtgt aattcgctgg attggtggtc
      901 aagctgaaca atcgatggta gaacgcgcta tggaagaaac caaaaaagca gcagaaactg
      961 ctacacacgc tacacaacaa gttatgaaag gcacaactga aggtattgtt tcggccggaa
     1021 aaatggcagt ggagaaaggc aaagctcctg ctgaaggcgg tggtggcggt ggtggtggtc
     1081 aaggtggagc acagggagtc agtggtctac agatgtaatg gaaaaccact taaggcagcc
     1141 aataaactaa ctttactctc caaccattta tatgcttaaa taaacctttg atttcgtgct
     1201 aatacacaac gaggtttata tggcaaacag tgttcctcaa cttaatgtag gtaaatctag
     1261 ggaaattacg aattcaaaaa aaaatcaacg cactccctct ttcaaagaac aagaacaagt
     1321 gcgcgaggaa ttaaaaaaac gccttaagaa atatgcagaa accacccctt ctatggtcgc
     1381 ttcaaatggc caaagtttcg gggatagtat tattcacatg aataaattca atcaagaagc
     1441 aatcgctgtg caaatgttga ttgaagacgc tattttcatc atgcaattat tatttgcact
     1501 ctgtggtgca aagcttgtga tgatagatgc acaagaatgg aatcaagcac gtcaaaaaga
     1561 tttggcctta cgggaaaact tgcgggcaaa aaaagaaaat gtgcaaaacg aaattgccga
     1621 aattgaaaaa actttggcag aaaagaaatt agaaacagaa gaaaaagacc ttaaattaaa
     1681 atcgaatcaa gaagaattaa ataacattaa aaaacaacgt gtagctttgc gtgatcctaa
     1741 taacggtgcc ataacagatg ctgcaaaaga agcagagttg gtccagaaaa ttaaagattt
     1801 gttagaagtc caaaaaccgt atacagaata ttttgaggaa gtaaaaaaac ttgaagatcg
     1861 taaaaaatcg cttcaagatg aactgaagca tgtaaatgaa caacaagaaa cgacttatga
     1921 taaaacagaa tatccctatc ctactggaaa cgacgcggaa tcaaaacgca tacgtttaga
     1981 tacggaacga cgtttagcag aacgaaaaat ttatcccata aagtggatga aaacaccaac
     2041 gggcccaaaa tatgaattcg aaacggaaaa tggtccaatt gtgaatccga ccgctattac
     2101 ctatgatatt gctgtcgcta atggattaac gccaaaccca gaatacggcg tttcgattcg
     2161 caaagtagtc attgataata tggtaaaatt ctggggaatg gcgccagatt ggctatatta
     2221 tattgagcat caaaataggc ccgaactgcg ccaagaaaaa gcgcaaaatg ataaagatga
     2281 tcccattttg gctagaaaca atatgcctgc gcctaaacca cctggtcgat aataaaaaat
     2341 cacc
//
LOCUS       DASTIB010000091         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_159128, whole genome shotgun sequence.
ACCESSION   DASTIB010000091 DASTIB010000000
VERSION     DASTIB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159128"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFP93_01560"
     CDS             <1..233
                     /locus_tag="VFP93_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016955406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HET9843329.1"
                     /translation="ARIRLACRRGMLELDLFLLPFFEAVYSTLTEQEQEIFQSMLQQS
                     DQQLQRWLLGMEFPPQELQELIAKIRYHKKSS"
     gene            698..1279
                     /gene="rpoE"
                     /locus_tag="VFP93_01565"
     CDS             698..1279
                     /gene="rpoE"
                     /locus_tag="VFP93_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HET9843330.1"
                     /translation="MPNGVDSELVKLVQEGDKQAFDLLVQKYQFRILKLISRYVKDPA
                     EAMDIAQESFIKAYKALDKFRGDSAFYTWLYRIAINTAKNHLVLQNKRLVEADVEGID
                     AEYTLNRSFRDFTAPERIVIKNELEEVILDVIQHLPKELKTAITLREMEGLTYEEIAG
                     VMACPIGTVRSRIFRAREAIERRIKPYMNKLDN"
     gene            1289..1564
                     /locus_tag="VFP93_01570"
     CDS             1289..1564
                     /locus_tag="VFP93_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RseA family anti-sigma factor"
                     /protein_id="HET9843331.1"
                     /translation="MSVNMERYRVLLSSWVDGEKISKQEEKALIKALRQHAQLRDYWF
                     NLQTIRSILRGDGLMPDFTQFLKTPWKEYEYIALNLKPSPKKAKKRS"
     gene            complement(1590..2258)
                     /locus_tag="VFP93_01575"
     CDS             complement(1590..2258)
                     /locus_tag="VFP93_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9843332.1"
                     /translation="MNKIIKSLALCSILFPVAQSIANPYAGIEVGRGGNIKFNKMENQ
                     FESTKNTTWAARGFMGYKLTQNAPVNYGVETGFNQNEKAHALAHTSEASLNSKVKSRS
                     VDMLAVVDMPVITKVTVFGKGGVAVVEQKTISRAKLNQAVNAPNPFLSPNIAVVENTA
                     TSHRILPKGVVGLGYQLASNQSIDLSYNYVLGKRKAAFNPETSSYKVLAQDSLNLGWS
                     YHFA"
     gene            2536..>3138
                     /locus_tag="VFP93_01580"
     CDS             2536..>3138
                     /locus_tag="VFP93_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9843333.1"
                     /translation="MYHFSFSLKRCIITLILSLTFIALPAVSYNLPDFSKLVEKSSPA
                     VVSILTFDSTKATQQLIPDDLRDDIENTPLMDMLREMYGEQVEEKLYGKEGQPGLGSG
                     SLISEDGYIITNYHVVNGATKIYVQLKDRSQFQAHVIGFDPGTDLALLKIDGDNLPYL
                     KFSNEPVKVGQWVVAIGAPFGFEDTLTVGVVSAKQRSLGQE"
BASE COUNT          978 a          570 c          596 g          994 t
ORIGIN      
        1 aggctcgtat acgtttagct tgtagacgcg gcatgcttga acttgattta tttttactac
       61 ccttttttga agcagtttat tcaacactaa ccgagcaaga acaagaaatt tttcaaagca
      121 tgttgcagca aagtgatcaa cagctacaaa gatggctttt aggcatggaa tttccacctc
      181 aagagttgca ggaattaatt gcaaaaatcc gctaccacaa aaaaagcagt taagctttac
      241 actttttatc gtattgggtt gtgggcttcc ttaagtttaa gcatgcaaac gctctggtat
      301 tgtgccttac cttcgcattt acttactgta attggttcag tgtcaatgtt tattataatg
      361 ttttggtttt cgcaatattt agtgcaatct ttcgttattc acaatacttt tatcacagca
      421 gaatatctaa atcatcaaca acaaaaattt aggatccagc gcgtttgccg atctccttgg
      481 attattattt ttgatactga agatctggca aataataaac cgcattgttt cgtattcttt
      541 aaatttgaaa tgttaccttt cgattttgct tttttaaacc gatttttttg ggtttaattc
      601 gtgatcctgc gcctattatc aacccaaatg cgcttgaagc cgataacaat actgaagtat
      661 gataattatc tgacttataa aaatcgaggc gtaacctatg ccaaatgggg tagattcgga
      721 gttggtcaaa ttagttcaag aaggagacaa acaagctttt gatctattag tgcaaaaata
      781 tcaatttaga attcttaaat taatttcccg gtatgtaaaa gatccagccg aagcgatgga
      841 tatagcgcaa gaatctttta tcaaagccta caaagcactg gataagttta gaggcgatag
      901 cgctttttat acatggcttt accgcattgc tatcaatacc gcaaaaaatc acctagtctt
      961 acaaaataag cgactggtag aagccgatgt tgaaggaata gatgctgagt atacgttaaa
     1021 ccgtagtttt cgcgatttta cagcgccgga gcgcattgtg attaaaaatg aactagaaga
     1081 agtcatttta gatgtcattc agcatttacc gaaggaatta aaaacggcaa ttactctaag
     1141 agaaatggaa ggtcttacct acgaagaaat tgcaggtgtg atggcttgtc ctattggaac
     1201 tgttcgttcc cgaatctttc gagctcgaga agctatcgaa cgacgcatta aaccctatat
     1261 gaataaactc gataattagg aggttattat gagtgtcaac atggagcgtt atcgagtatt
     1321 attgtccagt tgggtagatg gtgaaaaaat ctccaagcaa gaagaaaagg ctcttataaa
     1381 agctttacgg caacatgcac aacttagaga ttattggttt aatttacaaa ctatccgttc
     1441 catcttacgg ggtgatggac ttatgccaga ttttactcaa tttttaaaaa ctccatggaa
     1501 agagtatgaa tacatagccc taaatcttaa gccttcccct aaaaaagcaa agaaaagaag
     1561 ttgatctaaa ctagatcaac ttcaaagttt taggcgaaat ggtaactcca acctaaattt
     1621 aaactatcct gagctaagac tttgtaggaa gaagtttctg gattaaaagc tgcttttctt
     1681 ttacccaaaa cataattata gctaagatcg atactttgat tgctcgctag ttgatagccc
     1741 agaccaacta cacctttagg aagaatacga tggctggtag cagtattttc aactacggca
     1801 atatttgggc ttaaaaaggg attgggcgcg tttaccgctt gattcaattt agcgcgacta
     1861 atggtttttt gttcaactac cgcgacaccg ccttttccaa acacggtaac cttcgtgata
     1921 actggcatat cgacaacggc cagcatatca acacttctac tttttacttt ggagtttaac
     1981 gaagcctctg aagtatgcgc taaagcatgc gctttttcat tttggttaaa ccccgtttca
     2041 acaccgtaat ttacaggggc attttgggta agtttatatc ccataaaccc acgtgctgcc
     2101 caagttgtat ttttggtgct ttcaaattgg ttttccattt tgttgaattt aatatttcca
     2161 ccacgaccta cttcgatgcc tgcgtaaggg tttgcaatgc tttgggccac gggaaatagg
     2221 atagaacata gtgctaaaga ttttattatt ttattcatgg tacaaccttt attgttagat
     2281 tgatataaag ttcatccatg aacggacggc taacgctaac aaattttatt taaaatgaca
     2341 agcaaactga ttgctacaac taatagtgct taaaatcttt agatgttttc tacgacttta
     2401 gtattagaaa ttttacaaaa atgcctgata ttgttttgat ctagagtcaa aaagtaagtt
     2461 agtgatataa taaaattatt caaaaaagcc catatgtgtt cagtagtcct ccctggtggc
     2521 tgctgggaga gtagcatgta tcacttctca ttttctctaa aacgttgcat tatcacttta
     2581 attttaagct taacttttat tgctttacct gccgtttctt ataacttacc tgatttcagc
     2641 aagttggttg aaaaaagctc tccagcagtt gttagcattc ttacatttga tagcacaaaa
     2701 gcaactcaac aattaatacc cgatgattta cgtgacgata tagaaaatac tcccttaatg
     2761 gatatgctac gtgaaatgta tggggagcaa gtcgaagaaa aattgtatgg gaaagaaggg
     2821 caacccggtt taggttccgg tagcttaatc agcgaagatg gttacatcat tacgaattat
     2881 catgttgtaa atggcgctac taaaatctat gttcaactga aagatcgctc tcaatttcaa
     2941 gcgcacgtta ttgggtttga tcccggtacg gatttagccc tattgaaaat tgatggagat
     3001 aatttacctt accttaaatt ttccaatgaa cctgtaaaag taggacaatg ggtcgttgcc
     3061 attggtgctc cctttggttt tgaagatacc ttgactgtgg gagtagtcag cgcaaaacaa
     3121 cgcagcttag gacaagaa
//
LOCUS       DASTIB010000092         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_738611, whole genome shotgun sequence.
ACCESSION   DASTIB010000092 DASTIB010000000
VERSION     DASTIB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738611"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFP93_01585"
     CDS             complement(<1..989)
                     /locus_tag="VFP93_01585"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9843334.1"
                     /translation="MLTAKPSFSLKAQITMPGDKSISHRAILLGSLSLGTTVINNFLR
                     SDDCLRTIQAMQNLGVPIHIACDNQIEIQGVAIHNLKTPSQPIDCGNSGTLMRILSGV
                     LAATSFETHLTGDASLCNRPMDRIVEPLQKMGASITGVKKNGCVTPPLIIKGNPCLQS
                     IDYKLAVPSAQVKSCLLMAGLFAQGKTKVHEPEKSRDHTERLFPVFGGSITREDNVIG
                     ITPQVLQGASLTIPGDISSAAFFIVAATLIPNSHLVIKDVGLNPTRMGLLHILRLMGA
                     NIQTRIKEGFEPCGEITIRSSNLQGIEVPASLIVAAIDEFPALFIASAFAKGET"
     gene            complement(996..2093)
                     /gene="serC"
                     /locus_tag="VFP93_01590"
     CDS             complement(996..2093)
                     /gene="serC"
                     /locus_tag="VFP93_01590"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET9843335.1"
                     /translation="MKKKTKKNHFNKDHLPINFSAGPALLPRSVLQSIYSEWFDWYEG
                     MSILELSHRCEPVMAMHEQVLILLRELLELPSDFTILMMHGGGRGQFSAVPLNLLKQK
                     NQAFYLLTGFWGELAASACERYASVTKMPINIQDSLPIFSTEDFQNMAYAHYTDNETI
                     AGIEFRQQPKIPNIPVVCDMTSSLLGKVLQWDHLDCVYASAQKNLGIAGCTFVIIRKS
                     LLGDAHPLTPDILNYKAYAESNSLFNTPPVFAWYVSLKVLEWVKNQGGVASMEDLAKN
                     RSDKLYQFIDKSSLYQNTVPAPWRSTMNVPFLIKNHESDFFPEARKVGLYFLEGHRNV
                     GGARASMYNAMPLEGVDRLIEFMTNFEEKYC"
     gene            complement(2062..>4301)
                     /gene="gyrA"
                     /locus_tag="VFP93_01595"
     CDS             complement(2062..>4301)
                     /gene="gyrA"
                     /locus_tag="VFP93_01595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9843336.1"
                     /translation="VDGQGNFGSVDGDAPAAMRYTEVRMSKFAHALLADLDKETVDFA
                     PNYDETEFAPIVLPTRVPNLLVNGSSGIAVGMATNIPPHNITEVINGCLALIENADIT
                     IEGLMEFIKGPDFPTAASIHGRAGILEAYRTGRGRVYVRAKSEVEVDNSGKQTIVITE
                     IPYQVNKARLIEKIAELVKEKKVEGISALRDESDKDGMRIVIELKRNEIAEVILNNLY
                     AHTQLQSVFGINMVALINGQPRTMNLKEILEAFVRHRRVVVNRRTAYDLRKAQERAHL
                     LEGWTVALANIDEVIEAIKTSESPAQAKERLVGKVWQSGMVASLLEKAGVTATRPQDL
                     EEFYGQHTNGYQLSNQQAQAILDLKLHRLTGLEQTKITDEYEAIIGEIKELSAILHDP
                     VKLMDVIKSELVEVKETFGDERRTLILADKKDISEADLITEEARVVTLSQEGYVKTQP
                     LDTYSAQKRGGRGKMATSVKDEDYIKTLLIANTHDTILCFSNHGKVYWLKVYELPLGS
                     RTSRGKPIINCLPLEAGETISAILPLRDFDTGAYVCLATKAGTIKKVALSEFSRPRSN
                     GIIAIDLVEGDSLIGAELTHEKCDIMLISNAGKAVRFHEDDVRGMGRTARGVRGIKLQ
                     ENQEVIALIVVDPESELLIATENGYGKRTKLGEFPTKGRGGQGVIAIQTSERNGQVVG
                     AIQLKEMDEVMIITDGGTLVRTKAEGISLVGRNTQGVRLINLSNGEKCVGIQRVEEED
                     EEESL"
BASE COUNT         1183 a          946 c          851 g         1321 t
ORIGIN      
        1 gtttcacctt tagcaaatgc gctagcgata aaaagtgcgg ggaattcgtc aattgcagcc
       61 acgatgagcg aagccggaac ctcgatacct tgcaaattac tcgatcgaat tgttatttcg
      121 ccacaaggtt caaacccctc tttaatcctg gtttgaatgt tggcacccat aagacgcaaa
      181 atgtgcaata atcccatacg cgtaggattt agccctacgt cttttataac caaatgggag
      241 ttgggaataa gggttgccgc tacaatgaaa aatgcagcag aagatatgtc acccggtatg
      301 gttaaagagg cgccttgcaa aacctgcggg gtgatgccaa tcacgttgtc ttctctcgta
      361 atagaacccc caaatacagg gaataaacgc tcggtatggt cccgtgattt ttcaggttca
      421 tgtacctttg ttttaccctg cgcaaataat ccagccatca aaaggcaaga ttttacctgc
      481 gcacttggaa ctgccagctt gtaatcaatc gattgtaagc atggattgcc ttttattata
      541 agcggagggg taacacaacc atttttcttt acacctgtaa tacttgcccc cattttttgc
      601 agcggttcta caatacgatc cataggtcta ttgcagagtg aagcgtcccc ggttaagtgt
      661 gtttcaaaag aagttgctgc cagcacccca cttaaaatac gcattaatgt gcctgagttg
      721 ccacaatcga tgggttgaga aggagtttta agattatgaa tagctactcc ttgtatttca
      781 atttgattat cgcaagcaat atgaatagga acacccaaat tttgcatagc ttggatagtg
      841 cgcaaacaat catcactgcg taaaaagttg tttattactg tagtgccaag gctaagcgat
      901 cctaacaaaa tggctcggtg ggagatcgat ttgtcgccag gcatagttat ttgtgctttt
      961 aggctgaagg atggtttagc agttaacata gcaagctagc aatatttttc ttcgaaattt
     1021 gtcataaatt cgattaatcg atccacacct tctaagggca ttgcattgta catactggca
     1081 cgagcacccc caacattgcg atgtccctct aagaaatata acccgacctt ccgtgcctcg
     1141 ggaaaaaaat cagattcatg gttttttata agaaaaggaa cattcatcgt ggagcgccaa
     1201 ggtgcgggaa cggtgttttg ataaagagaa cttttatcga taaattggta aagtttatcc
     1261 gaacggttct tagcaagatc ttccatgctt gctacacccc cttgattttt aacccattct
     1321 aagactttta aacttacata ccaagcaaat acgggcggtg tattaaataa ggaattggat
     1381 tcagcataag ctttataatt taaaatgtcg ggtgtaagag ggtgtgcgtc cccgagtaac
     1441 gattttcgaa taataacaaa cgtgcacccg gcaataccta agtttttttg cgcactagca
     1501 tacacacagt ctaaatgatc ccattgcaga acttttccca acaaacttga agtcatatca
     1561 caaacaaccg gtatatttgg aatttttggt tgttgtctaa actcaatccc cgcgattgtt
     1621 tcattgtcgg tgtaatgggc gtatgccata ttctgaaaat cttctgtgct aaaaattggc
     1681 aaagagtcct gaatattaat aggcattttt gtgacgcttg cgtaacgctc gcaggcagaa
     1741 gcggctaatt caccccaaaa accagtcagt aaatagaaag cttggttttt ctgttttaag
     1801 aggtttaagg gtaccgcgga aaattggcct ctaccgcccc catgcatcat gagaatggta
     1861 aaatcgcttg gcaattcaag caattctctt agcaggataa gaacttgctc gtgcatagcc
     1921 attacaggtt cacaccgatg gcttaactcg agtatggaca ttccctcata ccaatcaaac
     1981 cattcagaat agatagattg caagaccgag cgtggcaata atgccggtcc tgcggaaaaa
     2041 ttaatgggta agtgatcctt gttaaagtga ttcttcttcg tcttcttctt caactcgttg
     2101 tattccaacg cacttttctc cattcgacaa attaattaat cgtacccctt gggtattacg
     2161 acctactaaa gaaatgcctt ctgctttcgt gcgaaccagc gtgcctccat ccgtaatgat
     2221 cattacctca tccatttcct tcagttgaat tgccccaact acttgtccat ttctttcgct
     2281 agtttgaatt gcaataacac cttggccgcc gcgtcctttg gtagggaatt cacccagttt
     2341 ggtccgtttg ccataaccat tttccgtggc aattaataat tcactttcag gatcgaccac
     2401 aatgagcgct atgacctctt ggttttcttg taatttaatt cctcgaacac ctcgggcggt
     2461 tcttcccatg cctcgaacat catcttcatg gaaacgcacg gctttacccg cattgctgat
     2521 aagcataata tcgcattttt catgggttaa ttctgcacca attaaactat ccccttcgac
     2581 gaggtcaatg gcgataatgc cgttggatct gggtctggag aattctgata atgctacttt
     2641 tttaatcgta cctgctttag ttgctaagca aacataagcg cctgtatcaa aatcgcgtaa
     2701 aggtaatatt gcagaaatcg tttcccctgc ttctaaaggt aaacaattaa taatgggttt
     2761 accacgactt gtacgtgaac ctaacggtaa ctcgtaaact tttaaccaat agactttgcc
     2821 gtggttagaa aaacacaaaa tagtgtcatg ggtattggca attaataatg tttttatgta
     2881 gtcttcatct ttaaccgacg ttgccatttt tccgcgtcca ccacgttttt gggcactata
     2941 agtatctaga ggttgcgttt ttacgtaacc ttcttgtgat aaagtgacaa cacgtgcttc
     3001 ttcggttatt aaatcggctt ctgaaatatc ttttttatcg gcaagaatta aggtgcgtct
     3061 ttcgtccccg aaagtttctt taacttcaac caattcactt tttatgacgt ccatcaattt
     3121 gacaggatca tgtagaattg cagaaagttc tttaatctct ccgatgatag cttcgtattc
     3181 atcggttatt tttgtttgct cgagccctgt taaacgatgc aattttaaat ctaaaatagc
     3241 ttgggcttgt tgattggaaa gttggtagcc attggtgtgt tgaccataaa actcttctaa
     3301 atcttgtggt ctcgttgcag taacacctgc tttttctaaa agggatgcaa ccatgcctga
     3361 ttgccaaact tttccaacta aacgctcttt tgcttgggcc ggtgattcgg atgttttaat
     3421 tgcttcaatg acttcatcga tattagcaag ggctactgtc catccttcta aaagatgggc
     3481 acgttcttgc gctttacgta aatcatacgc cgtacgtcga ttaactacga ctctacgatg
     3541 tcgtacaaat gcttccaaga tttcttttaa attcatggta cgcggttggc cattgatcag
     3601 cgctaccatg ttaatgccaa atacactttg taattgtgta tgtgcgtaaa ggttatttag
     3661 tataacttct gcaatttcat tgcgttttaa ttcgataaca atacgcatgc cgtctttgtc
     3721 ggattcatcg cgcaacgcgc taatgccttc aactttcttt tcttttacca actcagcaat
     3781 tttttcgata agacgcgctt tgtttacttg ataaggtatt tcggtaatga cgatagtttg
     3841 tttaccacta ttatccacct ccacttcact tttagcccgc acataaacgc gaccgcgtcc
     3901 ggtacgataa gcttcaagaa tacccgctcg gccatgaata ctcgcggcgg tgggaaaatc
     3961 aggacctttg ataaattcca ttagaccttc gatggtaata tcagcgtttt caattaatgc
     4021 taaacaacca ttaatcactt cggtaatatt atggggtgga atattggttg ccattccaac
     4081 cgcgatcccc gaggagccat tcaccaataa attaggaacg cgcgtaggaa gtacaatagg
     4141 ggcaaattcg gtttcgtcgt aattgggtgc aaaatcaacc gtttctttat cgagatcggc
     4201 taataatgca tgtgcaaatt tactcatgcg cacttcggta tatcgcatgg ctgcgggtgc
     4261 atcgccatcc acagaaccga agtttccttg cccatcgact a
//
LOCUS       DASTIB010000093         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_614471, whole genome shotgun sequence.
ACCESSION   DASTIB010000093 DASTIB010000000
VERSION     DASTIB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614471"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFP93_01600"
     CDS             <1..448
                     /locus_tag="VFP93_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843337.1"
                     /translation="NALQVAVAFGSYASVKLLIKAQANVHVTWNKKGKEYTLLDVLSN
                     YHNNVKNSDQNTFEKKLRLLCKLNVKYNHSHFFMFNEKSFEDTVASTKGPIVFSKFTP
                     SPNLPGIEQASVFVADEALVKSEKEANYQPGVPKFKPGFLNKGKGI"
     gene            468..1181
                     /locus_tag="VFP93_01605"
     CDS             468..1181
                     /locus_tag="VFP93_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843338.1"
                     /translation="MFQSILSAFSWSKNPASLLLSYLREGNVQEVHKILFSQNPPRLQ
                     DVQGTEGYKPNLLIMSIHAPAIVAALLDTYEDISGRDRSEAFLQAANGGHVQTIEVLL
                     DKSAAQISNIDKEYAFRLAVENNHIPLIVKLLTRCGNQINAQEKKFALLDAAERGFVV
                     AFFEIFTRVSDNLSNDDKNWVFEVIAENGYVDDLNLLLVSCIADKNQALVFSTDSTKA
                     REKPETIEREFSGPRLCRR"
     gene            complement(1242..2339)
                     /locus_tag="VFP93_01610"
     CDS             complement(1242..2339)
                     /locus_tag="VFP93_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843339.1"
                     /translation="MLTNAPLPTSHPSVDYSSTVLLFQFHISHPDFFHKTTIELDQNV
                     LEAKQFESQFGYVLGKVLRASVEKMQSIMHALKETAMNHLVYPLGRFFYLIDKDQLEQ
                     KLDNDMVDLYKFLERKIEAAKRKADKNRKPLMILIGENHYSPHSALVQSMILTIAKEK
                     LCMHSILTESFFESAYTTLHAKSILTPSLLIEEQAKKMNLTKIPLDYALCRFLEERKP
                     ECPMNTPNECKTNVLYDQVSSPMTNEALFVRNKVMANVALHAKVNETVTIVGGGHLKG
                     LIEDTALKENYELLPINSFFHDNVFLNVMRLILEHEVPELNYLENSDKVLQTQIPSLT
                     LALSDFPPEKLVEKAQSIKPKKIFEIAKLKA"
     gene            complement(2638..>2859)
                     /locus_tag="VFP93_01615"
     CDS             complement(2638..>2859)
                     /locus_tag="VFP93_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843340.1"
                     /translation="VSVPVLFLWGDQKLATNQLQPQSMTENRVRNKPETSSPSLLFGF
                     KPSADIDGLDSLKNDVNSYPSSKTLPRPQ"
BASE COUNT          894 a          463 c          564 g          938 t
ORIGIN      
        1 gaatgcattg caagttgctg ttgcttttgg aagttatgcg tcggtaaagc ttttgattaa
       61 agcgcaagca aatgtgcatg ttacgtggaa caaaaaaggt aaagagtata ctttgttaga
      121 tgttcttagt aattatcata acaatgtaaa aaatagcgat caaaatacat ttgagaaaaa
      181 attgcgacta ttatgtaaat taaatgttaa atataatcac tcacattttt tcatgtttaa
      241 tgaaaagtct ttcgaagata ccgtagcttc aaccaaagga cccattgtgt tttcgaaatt
      301 tacgccttct cctaatcttc cgggaattga acaagctagt gtttttgtcg cagatgaggc
      361 acttgtaaag tctgaaaaag aagcaaatta tcaacctggc gttccgaaat ttaaaccggg
      421 ttttttgaac aaaggtaaag gtatttagaa ttcgtgcggt ggtgtatatg tttcagagta
      481 tactttcagc tttcagttgg tcgaaaaatc cagcttcttt gttgttgtct tatcttcgag
      541 aaggaaatgt acaagaagtt cataagatct tgttttcaca aaacccccca cggttacagg
      601 atgtacaagg tacagaaggt tataagccta atttattaat tatgagcatc catgcaccgg
      661 ctattgtggc tgcattgcta gacacttatg aagatattag tggtagggat agaagcgaag
      721 cttttttaca agcggcaaac ggtgggcatg tgcaaactat tgaggtcctc cttgacaaaa
      781 gcgccgcaca aataagcaat attgataaag aatacgcttt tcgccttgca gtagaaaata
      841 accatattcc ccttattgtg aaacttctta ctcggtgtgg aaaccagata aacgcgcaag
      901 aaaaaaaatt cgcactttta gatgccgctg agcgtggctt cgttgtagca ttttttgaga
      961 tttttacgcg cgtaagcgat aatctaagta acgacgataa aaattgggta tttgaagtga
     1021 tcgctgaaaa tggttacgta gatgatctta atctgttatt agtctcctgt attgcggata
     1081 aaaatcaagc gctagtattt tccactgatt ctacgaaagc acgcgaaaag cccgagacta
     1141 ttgagagaga gttttcaggc ccaagattgt gtagaagata gtgtaaattg agtgctgaaa
     1201 agaatataaa ctgttcggaa atttcagcac ttggttttaa ctcaagcctt aagctttgca
     1261 atttcaaata ttttttttgg tttaatcgat tgcgcttttt ccactaattt ttcgggagga
     1321 aaatcgctga gtgctaacgt taaactcggt atttgcgttt gaaggacttt atctgaattt
     1381 tctaaataat ttagctcagg tacttcatgt tctaaaatta ggcgcattac attgagaaac
     1441 acattgtcgt gaaagaaaga atttataggt aatagttcat aattttcttt taatgcggta
     1501 tcttctatca ggccttttaa atgtcctcca cccacaatag taactgtttc atttactttc
     1561 gcatgaagtg ctacattcgc cataacctta ttacgtacaa aaagggcttc atttgtcatc
     1621 ggagaactga cttgatcata taaaacgtta gtcttacact cgttcggagt attcatagga
     1681 cattcaggtt ttctttcctc aagaaatcga cacagtgcat aatctagggg aatttttgtc
     1741 agattcattt tttttgcttg ttcttcgata agtaacgaag gggttaagat acttttagca
     1801 tgtaaagtgg tgtaagcgct ttcaaaaaaa gattctgtta atatactgtg catacaaagc
     1861 ttttctttgg caatagttaa aatcatactt tgtactaaag cagaatgcgg actatagtga
     1921 ttctcgccaa ttaaaatcat caaaggctta cgatttttat cggcttttct tttcgcagct
     1981 tctattttac gttctaaaaa cttatataaa tcaaccatgt cattatctaa tttttgctct
     2041 aattgatctt tatcgataag gtaaaaaaat ctccccaaag gataaactaa gtgattcatg
     2101 gcggtttctt taagagcatg cattatagat tgcatttttt ccacgctggc acgtaagacc
     2161 ttgcccagca catagccaaa ttgagattca aattgtttcg cttcaagtac attttgatct
     2221 aactctattg tggttttatg aaaaaaatct gggtgggaaa tgtgaaactg gaaaagcaaa
     2281 acagtcgaag aataatctac actagggtga gaagtgggta aaggtgcatt tgttaacata
     2341 taaaagcttt gaattatttt ttgtagtata gcgagataag gtctaaaata ttttttgatt
     2401 tatcgacaaa tttgtacttt ttatctgaac acaagatgtc gtgactgcgt gagcagtatt
     2461 cttctaaaca caatgcatga atacttaaaa agttaggact ttggggtttg taatcaaatt
     2521 ttaagtttga aagcaatatg tgaatctgca cgtattaaat gaatgggccg tttggggaat
     2581 cagtaaccaa acacgtgctg cgattttcga tcgcggcacg ttagtgattt ctttacttta
     2641 ttgaggacga ggtaaggttt ttgagcttgg ataagagttt acatcgttct tgagggaatc
     2701 taagccatct atatcagccg atggtttaaa accaaaaagc aaagaaggag aagatgtctc
     2761 aggtttattt cgaactctat tttcagtcat tgactgtggt tgtagttggt tcgttgccaa
     2821 tttttgatca ccccataaaa atagcactgg aacagaaac
//
LOCUS       DASTIB010000094         5368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_748215, whole genome shotgun sequence.
ACCESSION   DASTIB010000094 DASTIB010000000
VERSION     DASTIB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748215"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="bioB"
                     /locus_tag="VFP93_01620"
     CDS             <1..924
                     /gene="bioB"
                     /locus_tag="VFP93_01620"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET9843341.1"
                     /translation="DEAKQLYALPFLELLYRAQTIHREHHDNAKMQLAALYNVKTGAC
                     PEDCGYCTQSRHYKTGLKKESLVSVEKTLEQALEAKNNGATRFCIGAAWRSPTDPQLN
                     KIIEMVKGVKDLGLESCVTLGMLQADQAKKLKEAGVDFYNHNLDTSPNYYPEVVTTRT
                     YADRIETLKNVREAGLKVCCGGILGMGESEQDRIELIHQLANLDPYPESVPINHLHPV
                     PGTRLGNSPRVADFDFIRIIALARIMMPKCYVRLTAGRVAMSQTMQALCFFAGANSIF
                     LGPKLLVVTNPELQADHQMLQNLGIEAQTCN"
     gene            915..1760
                     /gene="bioC"
                     /locus_tag="VFP93_01625"
     CDS             915..1760
                     /gene="bioC"
                     /locus_tag="VFP93_01625"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HET9843342.1"
                     /translation="MQLTHFSKKQVADSFSKYAPTYEEHAFLQKEIAYRLLEHSTLLK
                     MKPLRVLDLGCGTGILHDLIKKHYPTAEIVELDIAWGMVSRCAKNKKTWSFLKKKQII
                     QGDMEHLPFKNQSFDIVFSNCALQWSTDLSLLFKETERILQPKGAFFFATFGPDTLKE
                     VNALWQHVDNYPHVHTFLDMHPIGDLLLQVGFHDPVMDQEFLQVQYQSIEKLFSDLKC
                     TGARNLNPNRNKGLTTPKKFHMLKKTFQRVLESNKNFSCTYEVLYGHALKTNIKQIPT
                     HCLQL"
     gene            complement(1764..2732)
                     /locus_tag="VFP93_01630"
     CDS             complement(1764..2732)
                     /locus_tag="VFP93_01630"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9843343.1"
                     /translation="MQRNELILTDEEIKDCLHKNWKIKIHTLSYVPAGSTFGFKITLQ
                     NNSVVFLKIYKMFKAAKAIDRPNPQALPVLGHVLHKLKNIYKFDYFPRIIATHTAHWF
                     YEMQDYCFFLFDYIEGESPTYTPNTLNMLDFAKIVAMLHQIPMQAFPELAIERFDLNY
                     VGGLTQWLNPIHPTMDAIGLDMIMQLRAWHNNIKKGLRTLELLREHVLGLHLPFVITH
                     GDPHYKNIMQTPQKIYLLDWDNLKIAPKERDLWFYEFGPFKAVYERHGSSPPIHSDVC
                     RFYQVQRFFEDIRFYLEQTDCSIAQRKEYLKCFINHWGWNYCASIE"
     gene            2845..3462
                     /locus_tag="VFP93_01635"
     CDS             2845..3462
                     /locus_tag="VFP93_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET9843344.1"
                     /translation="MVLFLSGILIGLAIAAPVGPIGILCIQRTLRQGPIAGLLSGLGA
                     AFADAVYGAIAGFSLVSVSEFLLRFEAPIRILGGIILIVLACKTWIAKIRVSHDSDSA
                     NTLWKDFFSTFLLTLANPATIIAFAAIYAGFGMIDENASYWSASLLITGIFLGSLIWW
                     IFLCMVIHHIRHRLNEKAIHYINHLSSIILLVFGLLAMGSGFLEN"
     gene            3705..4547
                     /locus_tag="VFP93_01640"
     CDS             3705..4547
                     /locus_tag="VFP93_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843345.1"
                     /translation="MQFSKKQIDHAIQCISSRLFEENTYTSEQRTWFVENFKALLKQI
                     TATPYTDLTIRIASNENKLRGFFSHCEKQGIAFNLKESYVLAPGRTMEYSLREDGSIG
                     ITTLFQKQLPSKNNKHKSKQALQSFLQEEKKLYLEEIYETKEPDYLSSNDTDYSESDT
                     SSIPSMDAYLSDDELSSHGENSAEENYWTQEEYKAIFLAISKALKNAQLAKVETETHD
                     NLPSLCFDFKKKVEQKRETNQKHKNPIFLLKQWEVKPQKEEQVLEEIPKHLQRKRAFN
                     HPRR"
     gene            4550..>5368
                     /locus_tag="VFP93_01645"
     CDS             4550..>5368
                     /locus_tag="VFP93_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET9843346.1"
                     /translation="MITDQQKNAYLSKIGITDTIKVCKTDLKRLVEGHCFTFPYETFN
                     VHDSTFDHDPKKRSSLAFSTLFKKLLKENRGGRCVELNIFLQKMLQSFGFEVMPILPE
                     TLWHHEDKKKGERPKHSAALVTCEGQTFLVDAAFGGLGILSPLPLKPGTYPQYSETFK
                     LVAKTDYAYELYSKQEDQWKAIYGFDCKEASLAHYANIDAQNSEPLREKCYFKNYFIC
                     TKPFKIDNNKNGRVRLVNEEFMIYENGKMTLKQNITSQQQFHALAKDYFNIDLRG"
BASE COUNT         1747 a         1005 c         1016 g         1600 t
ORIGIN      
        1 gatgaagcaa aacaattgta cgcgcttcct tttttggaac ttttataccg cgcacaaacc
       61 atacacagag aacaccacga taatgcaaaa atgcaattgg cagcactcta taatgtcaaa
      121 acaggcgctt gtccagaaga ttgtggctat tgtacccaat cacgccatta taaaacagga
      181 ctaaaaaaag aaagtctggt ttccgtggaa aagactttag aacaagcttt agaggcaaaa
      241 aataacggcg ctacccgttt ttgcattgga gcagcgtggc gctcccctac cgatccgcaa
      301 cttaataaaa ttattgagat ggtaaaaggt gttaaagatc ttggattaga atcttgtgta
      361 acactgggaa tgcttcaagc cgatcaagca aaaaaactta aagaggcagg cgtagatttt
      421 tataatcaca acctggatac ctcgcccaat tattatcccg aagtggtaac cacacgcact
      481 tatgcggatc gaatagaaac attaaaaaat gttcgcgaag ctggtttgaa agtatgctgt
      541 ggcggcattt taggcatggg agaaagcgag caagatcgaa ttgagcttat tcaccaattg
      601 gctaatcttg atccgtatcc agaaagtgtc ccaattaatc atttacatcc tgtgccgggt
      661 acgcgcttag gaaattcgcc ccgcgtagcg gacttcgatt ttattcgaat tatcgctttg
      721 gcgagaatta tgatgccaaa atgttatgta cgtttaaccg caggtagagt cgcaatgtcg
      781 caaaccatgc aggcattatg cttctttgca ggagccaatt cgatattttt aggtcctaaa
      841 cttttggttg taaccaatcc tgaattgcaa gccgatcacc aaatgttgca aaatttaggt
      901 attgaagcac aaacatgcaa ttaacccatt tttctaaaaa acaagttgcg gattctttta
      961 gtaaatacgc acccacgtat gaagagcatg cgtttttgca aaaggaaatc gcctatcgtt
     1021 tgctagaaca cagtacacta ttaaaaatga agcctttacg cgttttagat ctgggttgtg
     1081 gtacaggcat tttgcacgat ttaatcaaaa agcattatcc aacagcggaa attgttgagt
     1141 tagatattgc ctggggtatg gtctcacgtt gtgcgaaaaa taaaaaaact tggtcctttt
     1201 taaaaaaaaa gcaaattatc caaggagata tggagcattt gcctttcaaa aaccaaagct
     1261 ttgacatagt attttctaat tgtgcattac agtggtctac cgatctatcg cttttattta
     1321 aagaaaccga acgcatattg cagccaaaag gggctttctt ttttgcaaca tttggaccag
     1381 ataccctgaa agaagtaaat gctttgtggc aacacgttga taattacccc catgtacata
     1441 cttttttgga tatgcatcct attggggatc tgcttttaca agtggggttt catgatccgg
     1501 ttatggatca ggagtttcta caggtgcaat accaatcgat agaaaaatta ttcagtgatc
     1561 tcaaatgcac cggagcgcgc aatttaaacc ctaatcgtaa taaaggttta accacgccca
     1621 aaaaattcca catgttaaag aaaacttttc aacgcgtgtt agaatcgaac aagaactttt
     1681 cctgtaccta tgaagtgctc tatggacatg cacttaaaac aaatatcaaa caaattccta
     1741 cgcattgttt acaactataa agcttattcg atacttgcgc agtagttcca accccaatga
     1801 tttataaaac attttaaata ttcttttcgt tgtgctattg agcaatcggt ttgttctaaa
     1861 taaaaacgga tgtcttcaaa aaatcgttgc acttggtaaa atctacaaac atctgaatgg
     1921 ataggtgggc tactcccatg gcgctcatac acggctttaa atggcccaaa ctcataaaac
     1981 caaagatcgc gctctttcgg cgcaattttc aaattatccc aatccaacaa atagattttt
     2041 tgcggcgttt gcataatatt tttataatgg ggatccccat gcgtgatcac aaacggtaaa
     2101 tgtaaaccca acacgtgctc gcgcaacaac tctaaggtgc gaagtccctt ttttatgttg
     2161 ttatgccaag cacggagctg cattatcatg tcaagaccta tggcatccat ggtcggatgt
     2221 atcggattta accattgcgt taatccgcca acgtaattta aatcaaaacg ctctatggca
     2281 agttctggaa atgcctgcat aggtatttga tgcaacattg ctacgatttt cgcaaaatcc
     2341 aacatattta acgtattggg tgtgtaagta ggagattcac cttcgatgta gtcgaataaa
     2401 aaaaaacaat aatcttgcat ctcataaaac caatgcgcgg tatgtgtagc gattatgcga
     2461 ggaaaataat caaatttata gatatttttt agtttatgca aaacatgtcc taatacgggc
     2521 aaggcttgcg gattaggcct atcaatggct tttgctgctt taaacatttt ataaattttt
     2581 aagaaaacta cagaattatt ttgcaaagtg atcttaaatc caaaagtaga gcccgcaggc
     2641 acataagaca aagtatgtat ttttatcttc caatttttat gaagacaatc tttgatttct
     2701 tcatccgtga gtattaattc attccgttgc atcaaaatct tcctattgaa ttaagctaaa
     2761 taaattaatt cgatgtgtat tttctaggtt accctaggtc agactgttat aatttagaat
     2821 taatttggca aactctcggt atacatggtt ctttttctga gtgggatttt aattggttta
     2881 gccattgctg cacctgtagg gcctattggt attctatgca ttcaacgtac tctcagacaa
     2941 ggacctatcg caggactcct atccggtttg ggcgcagcat tcgcagatgc agtctatggc
     3001 gctatcgcag gatttagttt agtcagtgtt tctgagtttc tcttgcgctt tgaagcaccc
     3061 attcgtatcc tgggtggaat aatcctgatt gtattagcct gtaaaacgtg gatcgcaaaa
     3121 atccgggtgt cccatgattc ggattctgct aatactttgt ggaaagattt tttctcaact
     3181 tttttattaa ccctggccaa tcctgccact attatagctt ttgccgcaat ttatgcaggc
     3241 tttggcatga tagatgaaaa cgcaagttac tggagcgcct cgcttttaat taccggaatt
     3301 tttttgggat cgttaatatg gtggattttt ttgtgtatgg tgatccatca cattcggcat
     3361 cgtctaaatg aaaaagcgat ccactacatt aatcatttgt cgagcatcat tctgttggtg
     3421 tttggtttgc tagcgatggg tagtggtttc ttagaaaatt aatctaagcg ggttgttggc
     3481 tctctaagta ttgcttatac tatctacgtt caaatgctct aatcaagaga gcaaaccaaa
     3541 tacataactg caaacattcc tgggtatgaa aactccatga aaaaatgttg cactttcaaa
     3601 ctcggttctc ttccttatac atttcgcact tttgcgagtt tttttatttt cttcctaaat
     3661 ttatcgcttt tctatgaaac tacggattgt tgaaggacaa aattatgcaa ttttcaaaaa
     3721 agcaaataga tcatgctatc cagtgtattt cttctcgatt atttgaagaa aacacctata
     3781 caagcgaaca aagaacctgg tttgttgaga attttaaggc tttattaaag caaataactg
     3841 ctacacccta tacggatctc accatacgta ttgcaagtaa tgaaaataaa ctgcgtggat
     3901 ttttctccca ttgtgagaaa caagggattg catttaattt aaaagaatct tacgtattag
     3961 caccaggtag aactatggag tactccttaa gggaggatgg gtccattgga atcactactt
     4021 tgtttcaaaa acaattacct tccaaaaata acaagcataa atccaaacaa gctctgcagt
     4081 cttttttaca agaggaaaaa aagctttatt tagaagagat ttatgaaaca aaagagccag
     4141 actatctgtc atccaacgat acggactatt cagaaagtga tacttctagt ataccgtcga
     4201 tggatgcgta tttaagcgac gatgaattaa gctctcacgg tgagaacagc gcagaagaaa
     4261 attattggac tcaagaagaa tataaagcta tttttttggc tataagtaaa gcattgaaaa
     4321 acgcgcaatt agcaaaggtt gaaaccgaaa ctcatgataa ccttccatcg ctgtgtttcg
     4381 attttaaaaa aaaagtcgag caaaaacgcg aaacgaacca gaaacacaaa aatccgattt
     4441 tcttgttaaa acaatgggaa gtaaagccac aaaaagaaga acaagttctt gaagaaatac
     4501 ctaaacacct gcaacgtaaa agagccttta accatccgag gaggtgaata tgataacgga
     4561 tcagcaaaag aatgcgtatt tatcaaaaat tggcataacg gataccataa aggtttgtaa
     4621 gacagactta aagcggttgg tagagggaca ttgctttacc tttccgtacg agacattcaa
     4681 cgtacacgat agtacgtttg atcacgatcc taaaaaacga tcatcccttg cgttttcaac
     4741 cttgtttaaa aaattactta aagaaaatcg aggcggaaga tgtgttgaat taaatatttt
     4801 cttacaaaaa atgttgcaaa gctttggatt tgaagtgatg cccattttac cagaaaccct
     4861 ttggcatcat gaagataaga aaaaaggaga aagaccaaag catagtgccg ctttggttac
     4921 ttgcgaaggc caaacctttt tggtcgatgc tgcttttgga ggcttaggaa ttttatctcc
     4981 tttgccttta aagcccggta cctatcctca atattccgaa acatttaaat tggtagccaa
     5041 aacggattat gcctatgaac tatatagtaa acaagaagac caatggaaag ctatttatgg
     5101 tttcgattgt aaagaagcgt ccctagcaca ttatgcgaac attgatgcac aaaatagcga
     5161 accgttacgc gaaaaatgtt attttaaaaa ttattttatt tgtactaaac cctttaaaat
     5221 agacaataac aagaatggcc gtgtacgatt ggttaatgag gaatttatga tttatgaaaa
     5281 cggcaaaatg acgttaaaac aaaacataac atcgcaacaa cagtttcatg cattggcaaa
     5341 agattatttt aatatagact taagagga
//
LOCUS       DASTIB010000095         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1475822, whole genome shotgun sequence.
ACCESSION   DASTIB010000095 DASTIB010000000
VERSION     DASTIB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475822"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..969)
                     /locus_tag="VFP93_01650"
     CDS             complement(52..969)
                     /locus_tag="VFP93_01650"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9843347.1"
                     /translation="MPKTLSMIISGILVLVSIQTFAAFFRDMFQAVPAHKEPAPTAKL
                     GQKLFFDPILSAGNKYSCNDCHQKNKAFSGFSNQQDVPTLLNVTYMYTFFWNGRALTL
                     SEALEEHLLSPTLMGQEENTLIEALKSNPEYVSEFETAYENGIALSTVIDALASYLET
                     LTTPYSRFDQYITGNVNAFNEKERTGFELFKSYGCASCHQGRNVGGNLMQKFGIYRNY
                     FQNPSAYDLGLFQFTKNEEDRFVFRVPSLRNIALTAPYFHNGSAQTLADAIRIMGQYQ
                     LNREIPDQDVEKIEAFLQTLSGSMERLND"
     gene            1180..>2140
                     /locus_tag="VFP93_01655"
     CDS             1180..>2140
                     /locus_tag="VFP93_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843348.1"
                     /translation="METQDKLQPALGFEEILDLDESVSPASSLTPNKPYHEIEEFLNL
                     TWEELFLEPVYVEEEEEEEVAFGEEGFVESEFLDLDFEDLFNIPVRLTRLDMNNNEDT
                     AAGKVQSGGGKLAKLKKALSQNDMAETQTSDRGDIELTPLSDITISSEFLPPPTDIPT
                     NAAPFAPLFYLGALQERTGQVQTIVDNIFRLPSSLGANLYDSLIPHGGDGSPNITLFI
                     RPDSSQTKTTMQGSSKITITTSAGNTLEFYTADEGQHKFGDYIYTLKNPVFHDDAQSN
                     IPHEFEDDGDFSVIDSNRDNKPTVEEITNALKQGFANGNYPDAQ"
BASE COUNT          674 a          395 c          436 g          635 t
ORIGIN      
        1 aaaaaaataa ttataataag tacagcacca acaaatagct gggtaagacg gttaatcatt
       61 taagcgctcc atgctacccg aaagcgtttg caaaaatgcc tcaatttttt caacatcttg
      121 gtcggggatt tctcgattca attgatattg tcccattata cgaattgcat cggctaacgt
      181 ttgcgcggat ccgttatgaa aataaggagc tgtgagggca atattgcgta agcttggtac
      241 ccgaaataca aaacgatctt cttcattttt tgtgaattga aatagaccaa gatcataagc
      301 cgacgggttc tggaaataat ttctataaat accaaatttt tgcatcaaat tgccacctac
      361 gtttcttcct tggtgacagc ttgcgcaccc atagctttta aaaagttcaa accccgtacg
      421 ttctttctca ttaaatgcgt ttacattccc agtaatatat tgatcgaagc gagaataggg
      481 tgtggttaat gtttctaaat aagaagcgag tgcatcaata actgtcgaaa gtgcgatccc
      541 attttcatag gcagtttcaa attcgctcac gtactcagga ttgcttttta aagcttcaat
      601 taacgtattt tcctcttgtc ccatgagcgt tggcgaaagc aaatgttctt ctaaagcttc
      661 tgacaaagtt agtgcgcgtc cgttccaaaa aaaagtatac atgtacgtta catttaacag
      721 ggtaggcaca tcttgttggt tagaaaatcc ggaaaaagct ttattttttt gatgacaatc
      781 gttgcaggaa tatttatttc ctgcggaaag tatgggatca aaaaaaagtt tttggccgag
      841 ttttgcggtg ggtgcaggct ctttatgggc tggaactgct tgaaacatat cccgaaaaaa
      901 agctgcaaaa gtttgtatgc tcactagaac caagattcca ctaataatca tggagagggt
      961 tttaggcata acgtattcgt ccttgaacaa taagaatagt gtaagctatt ttggtgtacc
     1021 ttccaatttt gcccatttgg tcagaatttt gcatctcaat cggtatttat aataaatagt
     1081 actaatgtcc agtatgcaat agggtcatta tttaataagt taatgtaatg gcttatcacc
     1141 aaaaatgttg tacatacatc ttttgaaaac aggtaagaaa tggaaactca agataaatta
     1201 cagcctgcgc ttggttttga agagatccta gacctcgacg aaagtgtatc tcctgcatct
     1261 tctttaaccc ccaataaacc ctatcatgaa atcgaagaat tcttgaattt aacctgggaa
     1321 gaattatttt tagagcccgt ttatgtagaa gaagaggaag aagaggaggt ggcttttggc
     1381 gaagaaggct tcgtggaaag cgagttttta gatttagatt ttgaggattt atttaatatt
     1441 cccgtgcgcc taacgcgctt agatatgaat aataacgaag atacggctgc gggaaaagtg
     1501 caatcgggag ggggaaagct tgcgaaatta aaaaaagcgc ttagtcaaaa tgatatggcg
     1561 gaaacccaaa cctcggatag aggggatatt gaattaacac ctctttccga tattacgata
     1621 tcgtctgaat ttttgccacc gcctacggat attccaacga atgctgcgcc ttttgcgcca
     1681 ttattttatt tgggagcttt acaagaaaga acgggtcaag tacaaacgat agtggataat
     1741 atttttcgct tacccagttc gttaggtgca aatctttatg acagtttgat tccacatggt
     1801 ggagatggca gccctaatat taccttattt attcgccccg atagctcgca aacaaaaacc
     1861 acgatgcaag gtagtagcaa aattacgatt accacaagcg cgggtaatac ccttgaattt
     1921 tatacggcgg atgaaggcca gcataaattt ggcgattata tttacacttt aaaaaatccg
     1981 gttttccatg acgatgctca atccaacata ccgcacgaat ttgaagacga tggtgacttt
     2041 tctgtaatcg atagtaatcg cgataataaa cccaccgtag aagaaattac taatgcttta
     2101 aaacaaggtt ttgccaacgg aaattatccg gatgcgcaag
//
LOCUS       DASTIB010000096         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_360747, whole genome shotgun sequence.
ACCESSION   DASTIB010000096 DASTIB010000000
VERSION     DASTIB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360747"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1107)
                     /locus_tag="VFP93_01660"
     CDS             complement(286..1107)
                     /locus_tag="VFP93_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9843349.1"
                     /translation="MSSLEGKTIFITGASRGIGRSIALKLATHGANIVITGKTVEKHP
                     RLEGTIHSVAKEVETHGGRSLAIQLDIRDETQIYDAIEHTIQVFGGIDVLINNASAID
                     LTNAEEITLKKFDLMMGINQRGMFASSQACIPFLKKSSNPHILTLSPPIDLNPRWFTN
                     HMAYTMSKYGMSLCTLGLSAELKKYNIAVNSLWPRTIIATAAIINLFPEDFVKHSRRP
                     EIVADATEIILQKSSKDFTGQFLIDEEVLREAGNKNLEHYNFAEGNTLTTDLFLT"
     gene            complement(1117..>2108)
                     /locus_tag="VFP93_01665"
     CDS             complement(1117..>2108)
                     /locus_tag="VFP93_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843350.1"
                     /translation="YFPNTSSEQSPNISYIDAWVQNRWHSLLLGVTESGVLFTYDLHN
                     RMLLWQLPVPVFTLVVSMYPFRDIKGDWLIAAVLASPVKQAEMVFFNLTNGKKILSIP
                     LSRLQIDPSQKVRLAGVDKLGRGFVDTLYLSTHNAVWKVMIHSPYKTDWYIQPKPAFF
                     SETGTLPSKAMVTRSLQGKSVTVTQVMHTKGGDRIVQWLDDLRLCQYPITLASPFPCL
                     NWVFASKGQLSNPVIRQKFIVISEQRDQKMNLHVLSKETGKIVDTNSVQWIYLPALPI
                     RNKSYLNFTPQILLPSSRPRMQSILVQRQDGAIGLGKSKIDYEDLGRITWWLM"
BASE COUNT          652 a          409 c          395 g          652 t
ORIGIN      
        1 tccaagcata acggcataat ctactttgtt taatgggtag attgctgaag gtaatgctta
       61 cgtcaaaaaa attaaatact actgaaaaaa gtaatcaatg cagcagattg cacagcattg
      121 tacacacttt caaatcctcg caaaagtgta gggggtccaa aaaaaatgca ttaaaccaac
      181 aaaggttgga cacgttaatg caaaattcac tcgagagcaa atgttacaaa tttaagaggt
      241 ggttaacaaa caatatctaa attgggtaca ataattatga tgtacttatg ttaaaaataa
      301 gtcagttgtt aaagtgttgc cttcagcaaa gttataatgc tcgagatttt tatttcccgc
      361 ttctcttaga acttcctcat caataagaaa ttggccggta aaatctttgc tggatttttg
      421 caaaatgatt tctgtggcat ctgccacgat ttctggtcgt ctactatgct ttacaaaatc
      481 ttctggaaat aaattaataa ttgcagcggt agctatgatg gttcttggcc acagcgaatt
      541 tacagcaata ttatattttt ttaactccgc tgataagcct aaggtacata aactcatgcc
      601 gtatttggac atagtgtatg ccatatgatt tgtaaaccaa cgtggattta aatcaatagg
      661 cggtgatagc gtcagtatgt gggggttgct ggattttttt agaaaaggaa tgcaagcttg
      721 cgaactagca aacattccac gctgattaat gcccatcatt aagtcaaatt tttttaacgt
      781 aatttcttcg gcatttgtta agtctattgc acttgcatta ttaataagta catcgatgcc
      841 accaaacact tgaatcgtat gttcaattgc atcgtaaatt tgcgtttcgt cacgtatatc
      901 aagttgaata gctaatgacc tgccaccatg ggtttcaact tctttagcaa ccgaatggat
      961 tgttccttct aatcgcggat gtttttcgac agttttgcct gttataacaa tgtttgcgcc
     1021 atgtgttgct aatttcaatg caatcgatcg accaatgccg cgggatgcac cagttataaa
     1081 gatagttttc ccttcaagtg agctcataac aaatccttac attaaccacc aagttattcg
     1141 gcctaaatct tcataatcaa tcttcgattt tcctaatcct atcgcgccat cctgcctttg
     1201 tacaagaata ctctgcattc taggtcggga agatgggagt aaaatttgag gcgtgaaatt
     1261 taaataactt ttatttcgaa tggggagcgc aggcaagtaa atccattgca cagaattggt
     1321 atctacaatt tttcccgttt cttttgagag cacatgcaag ttcattttct gatccctttg
     1381 ttcactgatc acgataaact tttgcctgat aacaggattc gataattggc ctttgcttgc
     1441 aaaaacccaa tttagacaag gaaaggggga ggctaaggta ataggatatt ggcacagtct
     1501 tagatcatcc agccattgca cgatacgatc gcctcctttt gtatgcatta cctgcgtaac
     1561 tgtaacgctt ttaccctgta aagatcgcgt aaccattgcc ttgcttggta atgtacctgt
     1621 ttccgaaaaa aaagcaggtt tcggttgaat ataccaatct gttttatagg gagaatgaat
     1681 cattactttc catactgcat tgtgcgttga taaataaaga gtatctacaa agcctcttcc
     1741 taatttatca actcccgcta aacgcacttt ttgggatggg tcaatttgaa gcctagaaag
     1801 tgggatgcta aggattttct taccgttagt gagattaaaa aaaaccattt cagcttgttt
     1861 taccggggag gccaaaaccg cagcaattaa ccaatcgccc tttatatctc ggaatggata
     1921 catgctaacc actagggtaa aaacaggaac aggcaactgc cacaacaaca tcctattatg
     1981 taaatcatag gtaaacaaaa ctccgctctc tgtcacaccc aataacaaag aatgccatcg
     2041 attttgaacc catgcgtcaa tatagcttat gttgggagat tgctcggaag aagtgtttgg
     2101 aaaatata
//
LOCUS       DASTIB010000097         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1225264, whole genome shotgun sequence.
ACCESSION   DASTIB010000097 DASTIB010000000
VERSION     DASTIB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225264"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFP93_01670"
     CDS             <1..549
                     /locus_tag="VFP93_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9843351.1"
                     /translation="RFVTHTLHALPTRECKVVVAGIINQIRTLQTKRGERMAFVTLDD
                     NTGTFEIAIFADLYQKRRDFLNKDDIVIVSGLLSVNAQQIRLRAEDILDLPHAREKYA
                     ARLRLIIKSSPQIDFENWIQGLQQVLGEEKGHCRIAVDYCQDNFHARLHLGKQWGVLP
                     SDNLINKLKECSEHIGLHMDYV"
     gene            738..1937
                     /locus_tag="VFP93_01675"
     CDS             738..1937
                     /locus_tag="VFP93_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET9843352.1"
                     /translation="MTEKIRFAFSFTSLTVGIAIFSMFFGAGNVVFPLSLGRSIGEYI
                     DYALVGLIITGIGGPLLGLFSTILFDGDCKRFFNRIGDLPAYLLVLLIIAIIGPFGAM
                     PRCFTVSYSAITPYLDISLLTFSILSGILVLLCVIKRDFILPILGNLLSPILLIALII
                     IIIKGLFTSQDPIITDFSRVQAFWQGVEVGYDTMDVLASMFFAVVVWDLLKERFNRAG
                     KDITPKALAPLCFMSSLIGGVFLALIYVGLSKVASENMQALQGVPQEALLSALSIYIL
                     GPKFAFIANIAVALACLTTVISLAATVADVIIVEFRNSRIGNGRGLHYNWLVTTIIVI
                     TVIFSNLGFAKIMGYLHPIISVCYPAVIVLAICNILYKLWGFSWVKVPVYSTFVVSLF
                     FHIYLNY"
     gene            2005..2511
                     /locus_tag="VFP93_01680"
     CDS             2005..2511
                     /locus_tag="VFP93_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843353.1"
                     /translation="MPTLLDIQLRGSVEEPFFYPQEVHLHENEFYLLVIENGFDYPYL
                     LVANQFAEAIVTQSIQGSPSVTQQSVALSPHSKVQWLLRTKKAGKFPFYAHSTQLQKV
                     KSPISVFFIGSEESKSNPESTPPSVDATQSHSVAHFLSSNPFEINQETQKSGSPTPRM
                     RNSGHLRD"
     gene            2803..>3215
                     /locus_tag="VFP93_01685"
     CDS             2803..>3215
                     /locus_tag="VFP93_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843354.1"
                     /translation="MSQDQPLWVFVKNPDPSDDSFELQQNSNTLEGKFIEDGLRLKFI
                     QLEPSILAVVQAISKAYQDHHQKFHKNSKVTFNLLCNTEEEARFFIQNTHDQFPLTSL
                     KFFSKNFNKIINKNSLLESILTEHKEKRPSRPSNS"
BASE COUNT          945 a          575 c          600 g         1095 t
ORIGIN      
        1 cgctttgtaa cacatacgtt gcacgcttta cctacgcgag agtgtaaagt ggtggtagct
       61 gggattatta atcaaattcg gacgttacaa accaaacgtg gtgagcgcat ggcttttgtg
      121 acattagatg ataataccgg aacttttgag atagcgattt ttgcagattt atatcaaaag
      181 cgtcgcgatt ttttaaataa agatgacatt gtaatcgtaa gtggtctatt gagtgttaat
      241 gcgcaacaaa ttcgattgcg agcggaagat attttagatc ttccacatgc ccgagaaaaa
      301 tatgctgcac gtttgcgact tattattaag tcatcacctc aaatagattt tgaaaattgg
      361 attcagggtt tgcaacaagt tttaggcgaa gaaaagggac attgtcgaat tgctgtagat
      421 tattgccagg ataattttca cgcacgattg catttaggca aacaatgggg ggtccttcct
      481 tctgataact taattaacaa acttaaagaa tgctctgaac acatcggttt acacatggat
      541 tatgtttagt cgagttatct ctcgaaaaag gttaaaaaaa ccacagttgt gttccataag
      601 cgctcatggt accgtgattt gtgatggtca aacaattatg ctgaatatgt tgccaaaaaa
      661 taataacaac ttcgctccgt tttacctgaa tcgcatttcg caagaacgtt caaaaacgtt
      721 ttagtaaggt aaatattgtg acagaaaaaa ttcgttttgc ttttagtttt acttctctaa
      781 ccgttggaat tgccattttc tccatgtttt ttggagcagg caatgtcgtt tttccactca
      841 gtttaggccg aagcattgga gagtatattg attatgctct tgtgggttta attattacgg
      901 ggatcggtgg ccctttattg ggtttgttta gtaccatttt atttgatggg gattgcaaaa
      961 gattttttaa tcggataggt gatcttccgg cttatttatt ggtgcttctc attattgcga
     1021 ttataggccc atttggagct atgccgcgct gttttacggt ttcatactca gccattacgc
     1081 cgtatcttga tatttcgttg ttaacgttta gtattttgtc tggaattctt gtgctcttat
     1141 gcgtcatcaa aagggatttt attttgccta ttttaggtaa tttattaagt cccattttat
     1201 taatagcttt gattataatt ataataaagg gacttttcac ctcacaagat cctataatta
     1261 ccgatttctc tcgtgtacaa gctttttggc agggggttga agtcggctat gatacgatgg
     1321 atgtgctggc ttctatgttt tttgctgtcg ttgtgtggga tttgttaaaa gagcggttca
     1381 atcgtgccgg gaaagatatt acaccgaaag cattggcacc cctatgcttt atgtctagcc
     1441 ttattggagg tgtttttctt gcacttattt atgtaggact cagcaaagtt gcttctgaaa
     1501 atatgcaagc tttacaagga gtaccccaag aagcattact ttcggcgctt tctatttata
     1561 tcttagggcc aaaatttgct tttattgcca atatagccgt agccctggct tgtttaacta
     1621 cggtcataag cttagctgcc acagttgccg atgtgatcat tgtcgaattt agaaattcac
     1681 gcatcgggaa tggacgtgga ttacattata attggcttgt gacaaccatt attgtgatca
     1741 ccgtaatttt ttcaaactta ggttttgcaa aaatcatggg atatttacac ccgataattt
     1801 cggtatgtta tcctgcggta atcgtattag cgatatgtaa tattttgtat aaactttggg
     1861 gcttttcttg ggttaaggta cccgtttata gtactttcgt agtatcgctg ttttttcata
     1921 tctatttaaa ttattaatat ttttatgcag tatcgaagtt ttgctttatg tttgtttttt
     1981 attgttttta atataaaagc agaaatgccc acgttgctag atatccaatt acgcggctca
     2041 gtggaagagc ctttctttta cccgcaagaa gtccatttac atgaaaatga attttattta
     2101 ttggtaattg aaaatggatt tgattatcca taccttttag tagcgaacca atttgctgaa
     2161 gcgatagtta cgcaatcgat tcagggcagc ccgagtgtta cacaacaaag tgttgcttta
     2221 agtcctcata gcaaagtaca gtggctctta agaacaaaaa aagcggggaa atttcctttt
     2281 tatgcgcatt ctacccaatt acaaaaagta aaatccccta taagtgtttt ttttattgga
     2341 tccgaggaat ctaaatctaa tccagaatca acaccaccgt ctgtggatgc aacacaatcc
     2401 cattctgttg cccattttct atcatcgaat ccttttgaaa taaaccaaga aactcaaaaa
     2461 agtggttctc ctactcctag aatgagaaat tcaggccatt tgcgagatta acgcctttca
     2521 tgctacgtaa cgccacttgt tcgcgatatc cattttctac aaaatgttca tgaagctcga
     2581 tatatcactc actctcctac gtaaccgcga cttgctcgcg ttatcatgtt tcagcaatta
     2641 ttaaaggaac tttttcccgt ttcacctgtc ggatgtccat tataagtaat aagtattcgt
     2701 gtggataaac aaataaagtt attaataaaa agtacagatg atgaaaaata agttaaccac
     2761 caaaaattac gtgctaactt ttttgattct ttaaaagatg ctttgtccca agatcaaccc
     2821 ctatgggtat ttgttaaaaa tccagatccc agtgatgata gctttgaact acaacaaaat
     2881 tctaacactc tagaagggaa atttatcgag gacggtttac gcctaaaatt tattcagtta
     2941 gaaccgtcta ttctcgcggt tgtccaagca atttccaaag cgtatcagga tcatcatcaa
     3001 aaattccata aaaattcaaa agttacgttt aatttattat gcaataccga agaagaggct
     3061 agatttttta tccaaaatac ccacgatcaa tttccgctca catccttaaa atttttctcg
     3121 aaaaacttca acaagatcat taataagaat tctttattag aaagtatttt gacagagcat
     3181 aaagaaaaaa gaccatctcg accaagtaat tctta
//
LOCUS       DASTIB010000098         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1285023, whole genome shotgun sequence.
ACCESSION   DASTIB010000098 DASTIB010000000
VERSION     DASTIB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285023"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFP93_01690"
     CDS             <1..552
                     /locus_tag="VFP93_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843355.1"
                     /translation="YKNLLYEQQTEYTMKVQIYKTLGLEPFVKLPIRHQITGNCSWAN
                     VEASVPSMLFMLLHDKSAGNNTSQPLVREVMKFYLIWKEWDKDRALEECIDDFAQASP
                     NRRKAKASLMGSVLFQACDPKNAKDVQRAKKILAILKNPEYHFVVRSYINVFLKSGKG
                     GQAGRQFHQLLQACGISPSQFEF"
     gene            complement(561..983)
                     /locus_tag="VFP93_01695"
     CDS             complement(561..983)
                     /locus_tag="VFP93_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9843356.1"
                     /translation="MYKAILFASDLTEQSYSIGKKAKDLARLFGGRVEVIHVIEPIGA
                     YGGGIYYLGDLSVEIEKLARESLNELANRLEISEGNRHLCMGQTKHEILRIAEEIHAD
                     LIMMGTHGAKGFAMLLGSTASSVLNSAKCDVLTIPIRE"
     gene            1090..1839
                     /locus_tag="VFP93_01700"
     CDS             1090..1839
                     /locus_tag="VFP93_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9843357.1"
                     /translation="MLDESLFTLVLFGIGVVFIWGPWLLAQKIRPFFGKKQLNHWLAQ
                     RNQSKKMVQAVKTLTALYANTNPARVSKYTRLKHNLKQDHFLYGEIDLITLVKLLDFI
                     QPKPQTIFYDLGSGAGKAVLMASLTYPFAKCIGIELLAPLHQLAQKKCTLLERLAREK
                     DKNFCSNLHFIQGNFLAIDFSDADIIFANATAYKGETWDNLVNKLKTVKRGCRIIVTT
                     FKLNSLDFKILYEGAELMSWGFCSVRIYEKL"
     gene            1855..>2262
                     /locus_tag="VFP93_01705"
     CDS             1855..>2262
                     /locus_tag="VFP93_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9843358.1"
                     /translation="MENLNLIQQIAIWALPVIFGITLHEVSHGWIAYRLGDKTAYLQG
                     RLTLNPLKHVDFMGTIVVPLLLLIASAGFLFGWAKPVPVDPRNLKNPKRDMAFVAAAG
                     PISNLLMAIFWALILKLGIYLYMRGVSGGEAIGY"
BASE COUNT          672 a          456 c          430 g          704 t
ORIGIN      
        1 tataaaaacc tgctttacga acaacaaacc gaatacacca tgaaagtgca aatttataaa
       61 actctcggtt tagaaccttt tgtaaagcta cccattcgcc atcaaataac cggcaattgt
      121 tcatgggcta acgtagaagc atcggtacct tctatgttgt ttatgttgtt acatgataaa
      181 tcagcgggga ataatacatc acaaccgctt gtgcgcgaag taatgaaatt ttatctcatt
      241 tggaaagaat gggataaaga tcgtgcttta gaagaatgta tcgatgattt tgcccaagct
      301 agcccaaacc ggcgcaaagc caaagcgtct ttaatgggat ccgtactatt tcaggcttgt
      361 gatcccaaaa acgccaaaga tgtgcaacgc gcaaaaaaaa tattagctat attgaaaaat
      421 ccggaatacc attttgtggt acgcagttat attaatgttt ttttaaaaag tggcaaaggc
      481 ggccaagctg ggcgccaatt tcatcaacta ctccaagctt gtggcataag cccttcccaa
      541 tttgaatttt aaccagcgtt ttattcccgg atcggtatag tcaaaacgtc acattttgca
      601 ctatttaaca cgctggaggc ggtagatcct aacagcatcg caaatccttt agcaccatga
      661 gtacccatca taattaaatc agcgtgaatt tcttctgcta tacgaagtat ttcatgcttg
      721 gtttgtccca tacacaaatg gcgattccct tccgaaatct ctaatcgatt agccagctca
      781 tttaaacttt ctcgcgctaa tttttcaatt tccacactga gatcgcccaa ataataaatg
      841 ccacccccat acgcaccaat cggctcaatc acgtggataa cttctacgcg ccccccaaag
      901 agtcttgcaa gatctttggc ttttttgcca atggaatagc tttgctcagt taaatcgcta
      961 gcaaatagta ttgctttgta cataatacgc tccttgtatc ttttattatt gtaccttggg
     1021 acaagaaagg cttgctagag cgaacctgga tgttatgatg aaaactatct ttgtattgag
     1081 gttttgtgtg tgctcgacga gtcgctgttt actctcgtat tatttggtat tggcgttgta
     1141 tttatttggg gaccctggct tttagcgcag aaaattcgac ctttttttgg caaaaaacaa
     1201 ttaaaccatt ggttagcaca acgcaatcaa tccaaaaaaa tggttcaagc ggtaaaaaca
     1261 ctaacagcac tttacgcaaa cacgaaccct gcccgagttt ccaaatatac gcgtttaaaa
     1321 cataatttaa agcaagatca tttcctgtat ggtgaaatag atttaatcac tttggttaaa
     1381 ctcttagatt ttattcaacc caaaccgcaa actatatttt atgatttagg atcgggggct
     1441 ggcaaagcgg tactcatggc atcgcttacc tacccttttg caaaatgcat cggcatcgaa
     1501 ttattagcgc ctttacatca actagcccaa aaaaagtgca ctttattaga gagacttgcg
     1561 cgagaaaaag ataaaaattt ttgctccaat ctacacttta tccagggaaa ttttttagcc
     1621 atagatttta gcgatgcgga tattattttt gctaacgcca ctgcttataa aggcgaaact
     1681 tgggataatt tggttaataa actaaaaact gttaaacgcg ggtgtcgtat cattgtaact
     1741 acttttaaat taaactcatt ggattttaaa atcctctatg aaggtgctga actcatgagc
     1801 tggggctttt gttcggttcg tatttatgaa aagttgtaga aaatttgagg tcttgtggaa
     1861 aatctgaatt taattcaaca aatcgccatt tgggctttac ccgtaatctt tggtattacc
     1921 ttacacgaag tatcgcatgg ttggatagct taccgattag gcgataaaac tgcgtatttg
     1981 caagggcgcc taacgctaaa tccattaaaa cacgttgatt ttatgggtac tattgtagtg
     2041 ccgctactat tattaattgc ttctgcaggt tttttatttg gttgggctaa accggttcct
     2101 gtggatccac gcaatttaaa aaatcctaaa cgggatatgg cctttgttgc cgcagccggc
     2161 ccaatctcaa accttttgat ggctattttt tgggcgctta ttttaaagtt aggaatatat
     2221 ctttatatgc gtggcgtatc cggtggtgaa gcaattggct at
//
LOCUS       DASTIB010000099         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_989069, whole genome shotgun sequence.
ACCESSION   DASTIB010000099 DASTIB010000000
VERSION     DASTIB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989069"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFP93_01710"
     CDS             <1..705
                     /locus_tag="VFP93_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HET9843359.1"
                     /translation="NAGKLIIPVCLVIGTLNSVTTDFKLKTQGSADTLLASFGKTLTP
                     WFAPMGIEQENWPATVGLFTGIMAKEVVIGSLNTLYTQSSEELTKPLNIKEEIFNALL
                     TIPQNFGELFGFVVEDHTMDKQIYGEMQTRFKGEIGAFAYLLFVLLYFPCVSATAAMI
                     KEVDKKWTLFSLTWTTLIAYAVSVSFFQLATFAQHPLTSTVWTLCMLGIIVVFILLLS
                     NYKMQRTLPTPIILVT"
     gene            complement(729..1202)
                     /locus_tag="VFP93_01715"
     CDS             complement(729..1202)
                     /locus_tag="VFP93_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9843360.1"
                     /translation="MATRNKQRKTLQRRTKTTHNTKRTAKPSNSKGTRSLTSTTLTSI
                     KKTGPIQVGKKPYTKSELIRTIAEQTGLNRKHIQAVLESMSSIINAHLKKHGPESFSL
                     PGLFKIMVVNKPATPARWGVNPFTGEKMKFKAKPATRKVKIRPLKGLKEMATKAA"
     gene            complement(1259..2479)
                     /locus_tag="VFP93_01720"
     CDS             complement(1259..2479)
                     /locus_tag="VFP93_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9843361.1"
                     /translation="MSKDDLVQVIDTLASQCVKCGLCLPHCPTYNLDQNENESPRGRI
                     SLLQALVQEKLPTSESVLVHLDRCLTCRHCERVCPAHVDYGNLISKGRYWVQRDKKRQ
                     NTSFKTKLLAYLVQTPRKIKWAGLFLYLLEITRLRALAQQLGIPSFLGLSNLDQLLPT
                     VPKQKSFRSFYAHRAPYKGSVGLFLGCLSRWCDREIPSEIINIVTRLGFDIYIPQDQG
                     CCGALAEHQGFHQDAKKLVALTQAIFEPLPIDTILTFASGCGAMLLEQSTLSVKDISD
                     FLLPYLKPEYLHPLPYRVALHTPCTLINGMHKATPPHQILQKIPGLACFLIKEDGMCC
                     GSAGSYMLEQPERAVLLANKIIDSIPFDTLDYLVTSNIGCALHFRRILNLRGIKLPVV
                     HPISLVAKAFLSRS"
     gene            2595..2903
                     /locus_tag="VFP93_01725"
     CDS             2595..2903
                     /locus_tag="VFP93_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9843362.1"
                     /translation="MSYYDKHVFFCVNQREPGHNCCGNHRAEELRNYMKKQLKGMKTK
                     RHFRVNLAGCLGRCEEGPVIVIYPDGIWYTYHDERDIDEIIETHLVRGEIVNRLKLIT
                     "
     gene            3005..3442
                     /gene="rplM"
                     /locus_tag="VFP93_01730"
     CDS             3005..3442
                     /gene="rplM"
                     /locus_tag="VFP93_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9843363.1"
                     /translation="MATFSAKSHEVKREWYVMDAKGKTLGRLCTEIARVLQGKHKPIY
                     TPHVDTGDGVIVINAKEIHVTGKKERDKIYYHHTGYPGGIKSNALGDVRAKYPERIIE
                     MAVKRMLPKGPLGRQMFKKLHVYSGAEHPHQGQVPQEYLTSNS"
     gene            3451..3894
                     /gene="rpsI"
                     /locus_tag="VFP93_01735"
     CDS             3451..3894
                     /gene="rpsI"
                     /locus_tag="VFP93_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9843364.1"
                     /translation="MAQQKLYSGTGRRKTCVARVYLTPGSGAITINESTLEDYFGGRK
                     AAHMVVRQPLALLNVENKFDIKVNVFGSGPMGQAGAIRHGITRALLQYDEEGQNSDDE
                     SVSGESNSWRKILRSAGYVTRDSRVVERKKVGHRKARKVEQYSKR"
BASE COUNT         1205 a          745 c          862 g         1271 t
ORIGIN      
        1 aatgctggga aattaataat tcctgtgtgt ttagtcattg gcacattaaa ttctgttacc
       61 acggatttta aactaaaaac gcaaggcagc gcagacacct tgttggctag tttcggtaaa
      121 acgctaacgc cttggttcgc gccgatgggt attgagcaag aaaattggcc agcaaccgtg
      181 ggtttgttta ccggtatcat ggcaaaagag gtggttattg gtagtttgaa tacgctttat
      241 acccaaagca gtgaagagct aacaaagcct ttgaatataa aagaggaaat tttcaacgca
      301 ttgttaacga ttccccaaaa ttttggcgaa ctttttgggt ttgtagtgga agaccatact
      361 atggataaac aaatttatgg agaaatgcaa acccgtttta aaggagaaat tggcgctttt
      421 gcatatctat tatttgtact tttatacttt ccctgcgttt ccgcgaccgc tgctatgatt
      481 aaagaagtgg ataaaaaatg gactttattc tctttaactt ggacaacatt gatagcgtac
      541 gcagtttcgg tgagcttttt tcaactcgct acctttgcgc aacatcctct cacttcgaca
      601 gtctggacac tctgtatgtt gggaattatt gttgttttca tactgttgct cagcaactat
      661 aaaatgcagc gtacgctccc cacacccatt attcttgtga cataaaaaaa gcccagagtg
      721 ggcttttttt atgcggcttt ggtcgccatt tcttttaagc cttttaaagg acgaattttt
      781 acttttcgtg tggctggttt cgctttaaat ttcatttttt cacccgtaaa tggatttacg
      841 ccccaacgtg cgggtgtggc aggtttgttc actaccatga ttttaaataa accaggtaga
      901 gaaaaagatt ctggaccgtg ttttttgaga tgtgcattta taatggagct catggactct
      961 aacacggctt gaatgtgttt gcgatttaat cccgtttgct ccgcaatcgt acgaatcaat
     1021 tccgatttcg tatagggttt ttttccaact tgaattgggc cagttttctt aatactggtt
     1081 aaggtcgttg acgttaaact tcgcgtacct ttagaattgc tgggttttgc agtgcgcttg
     1141 gtattatgcg ttgttttcgt tcttctttgt aacgttttgc gttgcttgtt gcgtgttgcc
     1201 atgtgacttt ccttgtatgt ggtaagaact ttataaccat agcatattta tttttgattc
     1261 acgatctact taggaacgct tttgccacta aagaaatggg gtgcactacc ggaagtttta
     1321 tacctcgtaa gtttaatatt ctccgaaaat gtagtgcaca acctatatta gaagttacca
     1381 ggtaatctaa cgtatcgaaa ggaatagagt ctataatttt attggctaaa agaaccgctc
     1441 tctctggttg ctctaacata taactgcctg ctgaaccgca acacatacca tcctctttta
     1501 tcaaaaagca ggctaatccc ggaatttttt gcagaatttg gtgtggaggg gtcgctttat
     1561 gcatgccatt tatcaaagta caaggggtat gcaaagcaac tcgataaggc aaaggatgta
     1621 ggtattccgg ttttaagtat ggtagtaaaa aatcactaat atcttttacg gataaagtgg
     1681 attgctctaa cagcatggca ccacacccac tcgcgaaagt tagtattgta tctatcggca
     1741 atggttcaaa aattgcttgg gttaatgcta caagtttttt agcatcctga tgaaatcctt
     1801 gatgctctgc taatgctcca cagcaacctt gatcttgagg aatgtaaata tcaaatccta
     1861 acctagttac gatattaatt atttctgaag ggatctctct atcacaccat cgacttaagc
     1921 agcccaaaaa taaccccacg gaccctttat aaggcgctcg atgggcatag aaagaacgaa
     1981 aagatttttg ttttggtaca gttggaagta attggtcgag attggatagg cccaaaaaag
     2041 atggaatacc taattgttgc gctaaagcgc gtaaacgggt aatttctaac aaataaagaa
     2101 agaggcctgc ccatttaatt tttctgggtg tttgaacgag ataagctaat aatttcgttt
     2161 tgaagctggt gttttgacgt tttttgtcgc gctggaccca gtatctccct ttcgaaatta
     2221 aattcccata gtctacatgg gcaggacaaa ccctttcaca atgacgacag gttaagcatc
     2281 tatccagatg cacaagcact gattcggatg tggggagctt ttcttgtact aatgcttgta
     2341 aaagagaaat tctaccccga ggtgactcat tttcattttg atcgagatta taggtagggc
     2401 agtgtggtaa gcataaacca catttaacac actggcttgc aagggtatca atcacttgta
     2461 ccaagtcatc ttttgacatg tagctctctg ataaaactcc attttgccca gcttaatgat
     2521 acctagtaat caggtttgat ctctagtttg caactacaaa ttatcttata atccagctct
     2581 tattagaggt agctatgagt tattacgata agcacgtctt tttttgtgtt aatcagcgcg
     2641 agcctgggca caactgttgt ggtaatcatc gggctgagga actgcgaaat tatatgaaaa
     2701 agcagttaaa agggatgaaa acaaaacggc attttcgcgt aaatctagct ggctgcttag
     2761 gacgttgtga agaaggcccc gtaatagtca tctatccaga tgggatatgg tatacttacc
     2821 acgacgaaag ggacatcgat gaaataattg aaacgcattt ggttcgcggg gaaatagtaa
     2881 accggttgaa gttaattact taacactata atatatggtt tattgtcagt aagtatgacc
     2941 tcttgtataa tccctcaact taaatgagat ctttataaaa actattatct ggagcaggaa
     3001 ggaaatggca acattttctg caaaatctca cgaagttaaa cgtgagtggt atgtaatgga
     3061 tgctaaagga aaaaccttag gcagactatg caccgaaatc gcccgtgtat tgcaaggcaa
     3121 acataagcct atttataccc cacacgttga tactggcgat ggggttattg tcattaatgc
     3181 caaagaaatc catgttacgg gtaaaaaaga gcgcgataaa atttattatc accacacagg
     3241 ttatcctggc gggataaaat ctaatgcttt aggggatgtt cgtgctaaat atcccgaaag
     3301 aattattgaa atggcggtta aaaggatgct tcccaagggc cctttaggtc gtcaaatgtt
     3361 taaaaagttg catgtttata gtggtgctga acatcctcat caaggacagg taccgcaaga
     3421 atatttaacc tcaaatagtt aggtttatat atggctcagc aaaaattata ttctggaacc
     3481 ggtagaagaa aaacctgcgt tgctcgcgtt tatttaacgc caggaagtgg tgcaataacc
     3541 attaacgaaa gcacgttgga agattacttt ggtggtcgta aagctgctca catggtggta
     3601 cgccaaccgc ttgcattact taatgtcgaa aacaaattcg atataaaagt aaatgttttt
     3661 ggtagtggtc ctatgggaca agctggagca atccgacacg gcattacacg agctctttta
     3721 caatacgacg aagagggtca aaactctgac gacgagagtg tatcaggtga gagtaattct
     3781 tggagaaaaa tactacgaag cgcaggatat gtcactcgtg attcgcgcgt tgtagagcgt
     3841 aaaaaagtgg gtcaccgcaa agcgcgtaaa gttgaacaat actccaagcg ataaaaaatt
     3901 tgtatacaaa aaaagcaagc ttagcttgct ttttttttgt ttaaaattgc gataaaaagc
     3961 tgaaattaat tttagacttc ttgtcttagt tagcgaccgt agggtatttt tgttttaata
     4021 ctttgctctt tgttagctat agttaattat ctttataaaa tatttcattg tagcttttgc
     4081 ttt
//
LOCUS       DASTIB010000100         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_644699, whole genome shotgun sequence.
ACCESSION   DASTIB010000100 DASTIB010000000
VERSION     DASTIB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644699"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /gene="pgi"
                     /locus_tag="VFP93_01740"
     CDS             <1..1316
                     /gene="pgi"
                     /locus_tag="VFP93_01740"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9843365.1"
                     /translation="RPALHTALRYALWGSEEEISADLRKSILSSFQTMEDWVNHLQGG
                     YHGVTGKPICHIIHLGIGGSDLGPRLLLEALKPFYQSTLDVRFASNVDPQELLEHLEQ
                     VDPERTLFILASKSFKTQETLESAQLAKAWLQKHLKIEQVYKHFLAITANISLAQQWG
                     ISKTHILPFWDWVGGRYSIWSCVGFPVALKIGMQHFKDFLAGGYKMDQHFKAAPIQHN
                     MPIILALLGIWNNNFLKAPTHAILPYQYALRSLPAYLQQLDMESNGKSVQHYGRGVEY
                     TTGPILWGQVGTNGQHAFYQLLHQGTHLVPADFILVAKPSSPGCQHMHDLLIANGLAQ
                     TQALMMGQESTVLHEWIPGNKPTNTLLLKELTPQSLGMLIALYEHKVYVQGVIWNINS
                     FDQPGVELGKKLAHTVAKNFTNLEIKEVDPSTIQLLKKIKEYQTA"
     gene            complement(1351..1608)
                     /locus_tag="VFP93_01745"
     CDS             complement(1351..1608)
                     /locus_tag="VFP93_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843366.1"
                     /translation="MRELQMMEVRTISGGLDSLDLGSVMGAWIGYEAGKQLYPYDLMG
                     FNIGGLAAGIGVGMLGAITGRFFAGLLDSYIDPLIDKATGH"
BASE COUNT          788 a          405 c          414 g          706 t
ORIGIN      
        1 accgtcctgc attgcataca gcgcttagat acgcactttg gggcagtgaa gaagaaattt
       61 ctgcagactt aagaaaaagt attttaagca gttttcaaac catggaagac tgggttaacc
      121 atttacaggg aggttatcat ggtgttacgg gtaaaccgat ttgtcatatt atacatttag
      181 gaattggtgg atccgatttg ggtccaagat tgttattaga agcactgaag cctttttatc
      241 aaagtaccct ggacgttcga tttgcgtcta atgtggatcc acaggaatta ttagaacact
      301 tagaacaagt cgatcccgag cgcacgttat ttattttagc ttcaaaatca tttaaaacac
      361 aagaaacctt agaatctgct caattagcga aagcgtggtt gcaaaaacat ttgaaaattg
      421 agcaagtata taagcatttt cttgctatta ctgcaaatat ctcgttagcg caacaatggg
      481 gaatctctaa aacgcatatc ttaccttttt gggattgggt aggaggtcgt tattccattt
      541 ggtcatgtgt tggttttcct gttgcgttaa aaataggcat gcagcacttt aaggattttt
      601 tagcaggtgg atacaaaatg gatcaacatt ttaaagcagc acctatccaa cacaatatgc
      661 cgattatttt agctttgtta gggatttgga ataataattt tttaaaagcg ccaacccatg
      721 ctattttacc ttaccaatac gctttacgta gtttgccggc gtatttgcaa caactcgata
      781 tggaaagtaa tggaaaaagt gtacaacatt atggaagagg agtggaatat acaacaggtc
      841 cgattctttg gggacaagta ggtaccaacg gacaacacgc tttttatcaa ttattacatc
      901 aaggaaccca tctggtacct gcagatttta ttctagttgc taaaccatct tcacctggtt
      961 gtcagcatat gcatgattta ttaatcgcaa atggattagc gcaaacgcaa gctttaatga
     1021 tgggtcaaga atctacggta ttacatgaat ggatcccggg gaataaacct acgaatactt
     1081 tgcttctaaa agaattgacg ccacaaagtt taggaatgct catagcttta tatgaacaca
     1141 aagtttatgt acagggggta atttggaata ttaattcttt tgatcagcca ggcgtagagt
     1201 taggcaaaaa attagcgcat accgtagcta agaattttac aaaccttgaa atcaaagaag
     1261 ttgatccttc caccatacaa ttattaaaaa aaatcaaaga atatcaaact gcctaaaact
     1321 taaaaaaagg ccagaaaatc tggccttata ttaatggcct gttgctttgt ctatgagtgg
     1381 atcgatataa gaatctaata aaccagcaaa gaaacgtcca gtaatagcac ctagcatacc
     1441 tacaccgata ccagctgcta aaccgccaat attaaaaccc atcaaatcat aagggtaaag
     1501 ctgctttcct gcctcatatc caatccaagc acccattacc gaacctaaat ctaaactgtc
     1561 tagcccgcca gaaatagtac gcacttccat catttgtagt tcacgcatat gacacctcat
     1621 tattacaatt gaacacccag tgattgtgtt ttggtaaggc tcactcactt acaagctatc
     1681 tgataaagta aaaattagat aaccatgtgt gtgacaatca gttggatgta tccattttta
     1741 tcttacaaaa gcaatgtaca atggtttcaa gttcacgtca aaatttgctg acaagttaga
     1801 ccattgaatg taataaataa attatggtta actgcaaaat ttttttggaa ataaactatt
     1861 tttaagtatt gaaataaatt tgcctaaaaa acatattcat ttttcgaaaa aaattaaaaa
     1921 cgattatagt ttgcagtctt tgtaaaatta gcagaaaaat ttcttcactt tttcgtcaat
     1981 tttttttgtc aataatttgc gcgttaaaac taaaaattat ttgttcttta taaaaatgcg
     2041 caatctaaag aattcacaca agataatttg taaaatactt ttttagcaga tatttcgcag
     2101 ataaaccaag agagctattc aatcaactac cgttatttgt tcaaaaacat gtatgcaatg
     2161 gcatagtgcg tgatgataaa atctgctaat ctttgcacac tagtcaatgt ctattctaag
     2221 cttagtgttg ttagtaagct ataaagaata ctgggaatag taacttaggg aagatggcat
     2281 acagctttag gcaatgaaga ggactggata tga
//
LOCUS       DASTIB010000101         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_94542, whole genome shotgun sequence.
ACCESSION   DASTIB010000101 DASTIB010000000
VERSION     DASTIB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94542"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFP93_01750"
     CDS             complement(<1..1138)
                     /locus_tag="VFP93_01750"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9843367.1"
                     /translation="MAEVSRRTLANAIRVLSMDAVEAANSGHPGMPMGMADIAQVLWQ
                     DFFNHNPKNPDWFNRDRFVLSNGHGAMLQYSLLHLTGYDLTIDDLKKFRQLHSKTPGH
                     PERGETPGVETTTGPLGQGLANAVGMALAEKVLANTYNRDTHTIIDHWTYVFVGDGCL
                     MEGISHEACSLAGTLGLGKLIVFYDDNGISIDGNVVDWFQDDTVKRFEAYGWHVIPSI
                     DGHDFQSIKKAIETARTQDTKPTLICCKTTIGYGAPTLAGSAKTHGAPLGAQEVAGVR
                     LKLGWSESPFVIPDIIYQAWNGSEKGEVLEKEWLKKLELYKKQFPELAEDLTRRIEGR
                     LPNNLSHIFEQLLQEAQTYPKEIATRKASHLVLSKIGPLCSELLG"
     gene            complement(1210..1755)
                     /locus_tag="VFP93_01755"
     CDS             complement(1210..1755)
                     /locus_tag="VFP93_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9843368.1"
                     /translation="MALMETPICEFGLKAPDFQLHDCFGIPFSRDKVKKSKGLLVIFM
                     CNHCPYVKAILPKLSEEVEQIQKLEIGVIAINPNDPTEYPEDSEDHMRNVAQHYNFSF
                     PYCFDPTQQVAQSYGAVCTPDFFGYNANCELQYRGRFEAPEGELWKAMELIAKTGLGP
                     RQQIPSMGCSIKWKKHHSSAE"
     gene            1907..>2569
                     /locus_tag="VFP93_01760"
     CDS             1907..>2569
                     /locus_tag="VFP93_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HET9843369.1"
                     /translation="MAKQTVFTSESVSEGHPDKIADQISDAVLDAILTQDPESRVACE
                     TFIKNSLIIIGGEITTQAKEFDWDPLIRKLIYNIGYVEHPGFDSQSCSILTSLSKQSP
                     DIARGVNSAHDREQGAGDQGIMFGYATNETDVLMPAPITYAHRLVQTQAKVRKNKQLP
                     WLRPDAKSQISIRYENQKPIAIDAIVLSTQHEPDVTQGDLIEGVMEEIIKPTLPKEWL
                     HKN"
BASE COUNT          763 a          564 c          499 g          743 t
ORIGIN      
        1 ctcccaagag ctccgaacaa agaggaccaa ttttcgaaag aaccaaatgg gatgcttttc
       61 tggttgcaat ttctttgggg taagtttgag cttcttgtaa aagttgttca aaaatgtgtg
      121 acagattgtt cggtaatcgt ccctcgattc ggcgtgttaa atcctccgct aattcaggaa
      181 actgcttttt atagagttcc aatttcttta gccattcttt ctctagaact tcgccttttt
      241 cactgccatt ccaagcttga taaataatgt caggaataac aaaaggtgat tcactccatc
      301 caagttttaa tcgcacccct gctacttctt gcgcccctaa aggcgcgcca tgagtttttg
      361 cacttcccgc tagtgtgggc gcaccatacc caatggtagt tttacaacaa attaatgtgg
      421 gctttgtatc ttgtgtgcgt gctgtctcaa ttgctttttt gatactttga aaatcgtgac
      481 catcaatgga aggtattaca tgccacccat aagcctcaaa gcgctttacg gtatcatctt
      541 gaaaccaatc tactacatta ccgtcgatag agattccatt atcatcgtaa aatacaatca
      601 atttgcctaa gcctaaggtt ccggctaacg agcaagcttc atgcgaaatt ccttccatca
      661 aacaaccatc accgacaaaa acgtaagtcc aatgatcaat aatggtatgg gtgtcgcgat
      721 tatacgtatt cgctaatact ttttcggcca aagccatacc tactgcattg gctaaccctt
      781 ggcctaatgg gcctgtagtt gtttcaacac caggcgtttc accgcgttcg ggatgacccg
      841 gtgtttttga atgcaattga cgaaattttt tgagatcatc gattgtgaga tcatatccgg
      901 ttaagtgtaa taagctgtat tgcaacatag ccccatggcc gtttgataat acaaatcgat
      961 cacgattaaa ccaatccgga ttttttggat tgtggttgaa gaagtcctgc catagtactt
     1021 gcgcaatatc tgccataccc attggcatac ccggatgtcc tgaattcgct gcttctaccg
     1081 catccatact taacactcga atagcatttg caagggtacg gcgagaaact tcagccatgg
     1141 tcaactccta gttataaaac ttaccaacat gcggccacaa caaggcccac agtatagcat
     1201 tgctctatgt tattcagcgc tagaatgatg ttttttccac ttgatagaac aacccatgct
     1261 aggaatttgc tgacgaggac ccaatcccgt ttttgcaatt aattccatcg ccttccacaa
     1321 ttccccctcg ggtgcctcaa aacggcctcg atactgcagc tcacagtttg cgttgtaacc
     1381 aaaaaaatca ggagtgcata cagcgccata ggattgggcc acttgctgtg ttggatcaaa
     1441 acaataagga aaagagaagt tatagtgttg agcaacattg cgcatatgat cttcagaatc
     1501 ttcaggatat tccgtaggat catttggatt gattgctata acgccaattt ctaatttctg
     1561 aatttgctca acttcctcac ttaatttggg taaaatagcc tttacgtaag gacaatgatt
     1621 gcacataaat ataacgagca atcctttact tttttttact ttatcccgac taaaaggtat
     1681 cccgaaacaa tcatgaagtt gaaaatctgg agcttttaag ccaaattcac aaatgggcgt
     1741 ttccatcaaa gccatacaaa ttccttatag aaaaagactc gctcgctttt ataacacaaa
     1801 cttgggtaaa tctcactggc agctctagag gtgatctgtt actctctgaa ttttcgatgg
     1861 attgatgatt gtactgcgag atagcatgga gttaatgagg aagactatgg ccaaacagac
     1921 cgtttttaca tctgaatccg tttccgaagg ccaccctgat aaaattgcag atcaaatctc
     1981 tgatgctgtg ttagatgcga ttctaacgca agatccagaa agccgcgtcg cctgtgaaac
     2041 ctttatcaaa aacagcctta ttattattgg aggagaaata acgacccaag cgaaagaatt
     2101 tgattgggat cctttgataa gaaaacttat ttataatata ggctatgtcg aacatccggg
     2161 ttttgattcg caatcttgct ccattttaac ctctttaagc aaacaatcgc cggatattgc
     2221 gagaggtgtg aattcagcac acgatcgaga acaaggtgcg ggtgaccaag gaattatgtt
     2281 tggttatgca accaatgaaa ccgatgtttt aatgcctgcc cctataacgt atgcacatcg
     2341 attggttcaa acccaagcaa aggtgagaaa aaataaacaa ttgccctggt tgcgtcccga
     2401 tgccaaaagt caaataagta ttcgttatga aaatcaaaag cccattgcca tagatgcaat
     2461 cgttctttct acccaacatg agccggatgt tacgcagggt gatttaatag aaggggtcat
     2521 ggaagaaatc ataaaaccaa ctttaccgaa agaatggctt cataaaaat
//
LOCUS       DASTIB010000102         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_740873, whole genome shotgun sequence.
ACCESSION   DASTIB010000102 DASTIB010000000
VERSION     DASTIB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740873"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFP93_01765"
     CDS             <1..238
                     /locus_tag="VFP93_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9843370.1"
                     /translation="WNLIWIAIVFGIGHILEGFVLTPLFLGDKLGLHPIAVIFAVLAG
                     GQLFGFVGVLLAIPITAILVVLLREPILTWAKTS"
     gene            243..950
                     /gene="hda"
                     /locus_tag="VFP93_01770"
     CDS             243..950
                     /gene="hda"
                     /locus_tag="VFP93_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HET9843371.1"
                     /translation="MIPFAQHQLALSLTFTDGAELTNFVVGKNSLALERVKLAASGLG
                     EFILYVWGATSVGKTHLLQGACNAATLNHGLSAIYIPLRQYKKLKVQDLAGLEQLDLV
                     CLDDIEHICGDPIWEEAIFYLYNRMRALNKHLLVASNGAASHLSCGLLDLKTRLGWGE
                     IYHLESLSDDEKMQALKVRAQQRGLLLSNDVLQYIFNRSNRSMGALYQNLEILDKASL
                     TLQRKLTIPFVKKILNM"
     gene            1167..>2011
                     /locus_tag="VFP93_01775"
     CDS             1167..>2011
                     /locus_tag="VFP93_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843372.1"
                     /translation="MRFTFMQDPLTNSLAALGWNKAKQMLSAVVDATKNLIDNTKSVV
                     SNIWNADYTQLKATQLVIYPAIKNTIVNNKNLILSAAASTLMLAVLGINARGRGFTDL
                     LALGYYTINAAGIAFPAFVMGKCIKEVYAEIKRLIAQGVEVYEEVDDRYHQGKKNYGS
                     VNRERLAKVIEYTDGNYWEWSDVTIKNIAKASLNLANKSLNLVNKVIKPSLNWFEKRK
                     FVNDKVKPVLNGVVKTINYASDKVVKPSLNFSKDKVIKPGLNFAKDNVVKPGLNWVFV
                     KPINYA"
BASE COUNT          651 a          322 c          394 g          644 t
ORIGIN      
        1 ctggaatctt atttggattg caatcgtatt tggaataggt catatattag aaggctttgt
       61 gcttacacct ttatttttag gggataagtt agggttacat ccgatagcgg taatttttgc
      121 agttttagcc ggtgggcaat tatttggatt tgtgggcgtt ttattggcca ttcccattac
      181 agctattttg gtagtattac ttcgagaacc tattttaact tgggcgaaaa cctcataaaa
      241 acatgattcc atttgcgcaa catcaactag cattaagtct cacttttacc gacggtgcag
      301 agttaaccaa ttttgtagtg ggcaagaatt cactagcgct cgagcgggta aaattagccg
      361 ccagtggatt gggtgagttt atcctttatg tttggggcgc tacatctgtg gggaaaacac
      421 atttattgca gggggcctgt aatgcagcta cgttaaatca tggcttatcc gctatttata
      481 tcccgttacg tcaatataaa aaattgaagg tacaagattt ggcgggttta gaacagctcg
      541 atctcgtttg cttggatgat attgagcata tatgtggtga tccaatatgg gaagaagcaa
      601 ttttttacct atataatcgg atgcgtgcac tcaataaaca tttattagta gcatcgaatg
      661 gtgctgcatc gcatttatct tgtgggttac tcgatttaaa aacgcgctta ggctggggcg
      721 aaatttatca cttagaatca ctttctgatg atgagaaaat gcaagcacta aaagtacgcg
      781 cgcaacaacg tggacttttg ttatcgaacg atgtcttgca atacattttt aatcgatcca
      841 atcgttcgat gggagcgctt tatcaaaatc tcgaaatttt agataaagca tctttaacct
      901 tacaacgaaa attaaccatc ccattcgtca aaaaaatctt aaacatgtaa tagggaaaat
      961 ttttaagttt tctttacagc caagaattaa ttcatcatca taaacattaa aaacaatagt
     1021 tttttattct cgaaataaga tagcattata aaaaaaattc gtcttatctg cttaagcgct
     1081 ccaacagtct aaccttactt gtttttccaa gactcttaac gaaaaatgcc aaaactttac
     1141 tatagaaaca tttaaaagag gctgacatgc gattcacttt tatgcaagat cctttaacaa
     1201 atagtttagc tgcattggga tggaataaag caaagcaaat gctttcagct gttgttgatg
     1261 caactaaaaa tcttatcgat aacaccaaaa gtgttgtatc taatatatgg aatgcagact
     1321 atacacaact caaagcaacc cagcttgtga tttatcctgc gataaaaaac acgattgtca
     1381 acaataaaaa tttaattttg agtgctgctg catctactct aatgttggca gtgttaggaa
     1441 taaatgcgcg tggtcgtgga tttacggatt tattagcatt aggatattat actatcaatg
     1501 ccgcaggtat tgcgtttcct gcatttgtaa tgggcaaatg tattaaagaa gtctatgctg
     1561 aaattaagcg tttgatcgca caaggtgtgg aagtttatga agaagttgat gatcgttatc
     1621 atcaaggtaa aaagaattat ggatccgtta atcgcgagcg tctcgccaaa gttatcgaat
     1681 ataccgatgg caattattgg gagtggagtg acgttaccat caaaaatatt gcaaaagcga
     1741 gtttaaattt agctaataaa agtttaaatt tagttaataa agttattaaa ccaagtttaa
     1801 attggtttga aaaaaggaag tttgttaacg ataaagttaa acctgtttta aatggggttg
     1861 taaagacgat aaattatgct agcgataaag ttgtaaaacc tagccttaac ttttctaagg
     1921 ataaagttat caaacctggt ttaaactttg ctaaagataa cgttgttaaa ccgggcttaa
     1981 attgggtgtt tgtaaaaccc ataaattatg c
//
LOCUS       DASTIB010000103         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1106031, whole genome shotgun sequence.
ACCESSION   DASTIB010000103 DASTIB010000000
VERSION     DASTIB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106031"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /locus_tag="VFP93_01780"
     CDS             <1..1813
                     /locus_tag="VFP93_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9843373.1"
                     /translation="GGWLTSFYSAIPVVIMSPLAFLSRPERWLWAIHYYKATLSAAPN
                     FAYELCVKKIQEDKIKEMDLSSWRLSFNGAEAVNPATLERFYKKFAPYGLKNTMIFPA
                     YGLAESTVALILPKPHQEPQIDSIDKEIFETEHKAVPVHDPKTPKLSFVSVGSPIPQH
                     QVKIVDDNYHEVADRTVGRLLFKGPSMMQGYYKNETATEKIKIEDWLDSGDYAYKDDN
                     SYYITGRRKDLIIKAGRNFYPEEIEKIVSQISGIRQGCVAAFGAIDFTLSTEKLVIVA
                     ETKETKKKFHADIIDEVQEQVTSALGVPPDDVILVSPGSIPKTSSGKLQRARCKQLYE
                     TKRLSAPKVPVWLQFVKLNIRALFYKIRNYFYLFSQILYTGYVFFLTLLYIPMLTLLL
                     WCMPKQFKRTFAKRAARIGLYISCLPIFVKNAQKWPKKGAHVYVVNHASYLDAVVLLA
                     TLPQNVLLVGKKEIMKMPLVGKLAQAVGILTVDRYDFSKNLEDTKKIENAVLTGQSIA
                     IFPEGTFSYVEGLRSFKLGAFRIAVDTGTPICPLVIRGTRKIFRGKQWLLTPGKIEVE
                     IGSTIVPQSKDWSEVTRLHSEAKAFIATNCGEPLVEF"
     gene            complement(1810..>2348)
                     /locus_tag="VFP93_01785"
     CDS             complement(1810..>2348)
                     /locus_tag="VFP93_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843374.1"
                     /translation="AEIAQIANQHDIIPIFTIIPTKETVYYDKLIQMRVKLPDDFHQL
                     VTLEKKYIKAFGNSVKQLPSTLYIDLLTSLQEALSLNGHLYPQNENGHPLSKGYDVIA
                     QALHQSLPTLPSKNIAGPVAIPVQNNIFHLYLIKDNKLWLIPKHKDLETNGIDLTEVT
                     ILKPRDIAHLQHAGSLFD"
BASE COUNT          744 a          411 c          474 g          719 t
ORIGIN      
        1 cggaggatgg ctcaccagtt tttatagcgc aattccggta gtcattatgt cacccttagc
       61 atttttgagt cgccccgagc gatggctttg ggctattcat tattataagg caacgttatc
      121 tgcggccccg aattttgctt atgaattatg cgtcaaaaaa atccaagaag ataaaataaa
      181 agaaatggat ttaagttctt ggagattatc ttttaatggg gcagaagccg taaatcctgc
      241 aactttagaa agattctata aaaaatttgc gccttacggt ttaaaaaata ccatgatttt
      301 tccagcgtat ggattagctg aatctactgt agcacttata ctaccaaaac cacatcaaga
      361 accacaaatc gatagtattg ataaagaaat ttttgaaaca gaacacaaag cggttccagt
      421 gcatgatcct aaaacgccca agctttcttt tgtatccgtg ggttctccca ttccacaaca
      481 ccaagtaaaa attgtggatg ataattatca tgaagtagcc gatcgtactg tgggccggtt
      541 actattcaaa ggaccttcca tgatgcaagg ttattacaaa aacgaaaccg ccacagagaa
      601 aataaaaata gaagattggt tagatagtgg tgactatgcc tataaagacg acaatagtta
      661 ttatataaca ggtaggagaa aagatcttat tataaaagca ggacgcaatt tttatccgga
      721 agaaattgaa aaaattgttt ctcagatatc gggaattcga caaggatgcg ttgcagcctt
      781 cggtgcaata gattttacgt tatcgaccga gaaactcgta attgtcgcag aaaccaaaga
      841 aaccaagaaa aaatttcacg cagatataat agatgaagtg caagaacagg ttaccagtgc
      901 acttggcgtt ccaccggatg atgtgatttt ggtaagtcct ggaagtattc caaaaacctc
      961 tagcggaaag ttacaaagag cgcgttgtaa gcaactttat gaaacaaagc gcctttctgc
     1021 tcctaaagtt cccgtttggt tacaattcgt taaattaaat attcgtgcac ttttttataa
     1081 aatacgaaac tatttttatc tttttagtca aatactgtat acaggttatg tatttttttt
     1141 aaccctattg tacattccta tgttaacact tttgttatgg tgtatgccta agcaattcaa
     1201 aagaaccttt gcaaagcgcg ctgctagaat aggcttgtat atttcctgtt tgccaatttt
     1261 tgttaaaaat gctcaaaaat ggccaaaaaa gggagcgcat gtctatgtag taaatcatgc
     1321 aagctatctg gatgcggtcg ttttgcttgc aacacttccc caaaatgtac tgcttgtcgg
     1381 aaaaaaagaa attatgaaga tgcctttggt gggtaaattg gcacaagcag ttggaattct
     1441 taccgtagat cgatatgatt tttcaaaaaa cttagaggat actaaaaaaa ttgaaaatgc
     1501 ggtgttaaca ggccaatcca ttgcaatatt tccggaaggt acctttagtt acgtggaagg
     1561 tttgcgatcc tttaagctgg gggcttttcg catcgcagtc gacacgggta cacctatttg
     1621 tcctttagtg atccgaggca cacgaaaaat ttttcgaggt aaacaatggc tgctgacgcc
     1681 tggtaaaatt gaggtggaaa ttggatctac tattgtcccg caaagtaaag attggtcaga
     1741 agtaacacga ctccattcgg aagccaaagc ttttattgcc acgaattgtg gagagcccct
     1801 tgtggaattt taatcgaata aagagcccgc atgttgcaaa tgtgctatat ctcgaggttt
     1861 taagatggta acctcggtta aatctatacc attggtttct agatctttgt gtttgggtat
     1921 caaccataat ttattatctt tgattaaata tagatgaaaa atattgttct gcacggggat
     1981 agcaaccggt cctgctatgt ttttgctagg taatgtaggc aaggattgat gaagagcttg
     2041 ggctatgaca tcatagcctt tactcaaagg atgaccgttt tcattttgcg gatataaatg
     2101 cccatttaaa gataaagctt cttgcaaaga tgttagtaaa tcaatataaa gagtacttgg
     2161 caactgttta acactattcc caaaagcttt aatatatttt ttttccaggg ttaccaactg
     2221 gtgaaaatca tcaggaagtt ttacgcgcat ttgtattaac ttatcgtaat atacggtttc
     2281 tttagtcggt ataatcgtaa atatgggtat aatgtcatgc tgattcgcta tttgggctat
     2341 ttctgcag
//
LOCUS       DASTIB010000104         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_182313, whole genome shotgun sequence.
ACCESSION   DASTIB010000104 DASTIB010000000
VERSION     DASTIB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182313"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2403
                     /locus_tag="VFP93_01790"
     CDS             <1..2403
                     /locus_tag="VFP93_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843375.1"
                     /translation="ARNKYNRTPLHIAVDFDHVELVRKLITEFHVDVNIQDNLDYKPI
                     LYHAIKNDRCFEIIRLLVQEGKAETHILDKNGNNLLHIAVQKNQIDTVRLLIKSDIDI
                     NAYNNFKVTALTFAIDLDLVEIVAILVGEGKADVSIKDTYQNNCLHQAVRLQRKEIVQ
                     ILISKGNANIEDRTPEGNTPLLIAALLGNQEIAHLILQCNADVNAQNNQGTTALHLAM
                     IPDDPTLALMLIREHDADVNVINNGGCTPLHIATGFSRTEIIQALINEKHADVNAKDH
                     DGYTPLAWAVINSNVPLIHRYINEFHADPMITDNFGNNVLALAAMDGLFRSVNALLDV
                     DALRNNPNAGEMLALNGNLTLARAAAEGNIEIVNRLLAYPEVEQSAAINNNAILRAAQ
                     ANNHVAVVERLMQIPAVRNIPIPELGLMQVAQFNENAMQTLDDNQQGMITIVKQHYQQ
                     KMQSIGQDAVLENLKKYLETQYDNKPAQDPSGIKLPLEPFNPHLPSQEPYYKNIYHTA
                     RRYLFLRPNPWIFPNANYTDELPDGRAASIPPMAKTDIAYFWLAASDTSYLPTAEFTH
                     KKVMKLFTRRLADIGRAHNWDKYRVNAAGKLEQYDDLEGDKPSCDVGINQRLVQSVMG
                     NPITQQPQARELNPSIFRNKFIENLLFQHEDYTDNLYARLNQLSLQLLSELKEALENE
                     VVLCEPLTDAQEACFYIPEEQIDAFIEEAKTWFSANRITAPLQTVKWLTRNYENYVEF
                     ANDLSNDVKKHFHNEVVSILETLILQKTPISEPEIIKTTDVEADSADDMEADKPRKRR
                     RL"
     gene            2944..>3786
                     /locus_tag="VFP93_01795"
     CDS             2944..>3786
                     /locus_tag="VFP93_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843376.1"
                     /translation="MKKALKYTLSALAAAMIAAPAQANPWNFINELTFKAALTYTEAS
                     GNTANYAYTGFPQTWRPLFVEPDHEWDYALGLEWRIPGSNTHVFFDFEHFRDDASETA
                     VGAGTFGALPVAGATAFGSLEHREKDFKLGLRHTLEFGPHFDLALGAAIDAAKIERRF
                     NEIGTDPVVGVGTRFTHEETDAIGPFFDVTGRAYPWGQGRYGNCWSLWARAGFGLLYA
                     EHDYTLTTAVPGLVLPSFDPEDTNSIITKVEADFGVEFGRVLRLDFINMSLAARLGVK
                     YINYI"
BASE COUNT         1219 a          696 c          748 g         1123 t
ORIGIN      
        1 gcaagaaata agtataatcg cacaccctta catattgccg ttgatttcga tcatgtagaa
       61 ctagttcgca agctcattac agaatttcat gttgatgtaa atattcaaga taacttagat
      121 tataaaccta ttttatatca tgctattaaa aacgatcgat gttttgaaat tattcgtctt
      181 ttagtccaag aaggtaaggc ggaaacccat attcttgata agaatgggaa taatttacta
      241 catattgccg ttcaaaaaaa tcaaatcgat acagtgcgtc ttttaatcaa gagcgatatt
      301 gatattaatg cttacaataa ttttaaagta actgcgctta cttttgcaat cgatctcgat
      361 ttggttgaga ttgtggctat tttagtgggc gaaggtaaag cagacgttag cataaaagac
      421 acttatcaaa acaactgctt acaccaagct gtgcgcttgc aaagaaaaga aatcgtacaa
      481 atactgattt caaaaggaaa tgccaatata gaagatcgta ccccggaagg caacacgccc
      541 ttattaattg cggctttatt aggaaatcaa gaaatcgcgc acctgatatt gcaatgtaat
      601 gcggatgtta atgcgcaaaa taatcaaggt acgacagcat tgcatctggc catgattcct
      661 gatgatccaa cattggcact aatgcttatt cgagaacatg atgcggatgt aaacgtaata
      721 aataatggtg gatgcactcc tttacatata gcaacagggt tttctcgcac tgagataatc
      781 caagctctca taaacgaaaa gcacgcggat gttaatgcaa aagatcatga tggatatacc
      841 ccgcttgcat gggcggtaat taatagtaat gtaccactga ttcatcgata cattaatgag
      901 ttccatgcag atcctatgat taccgacaat tttggaaaca atgtgcttgc acttgcagcc
      961 atggatggtt tgtttagaag cgtcaatgca ttgttagatg tggatgcttt acgtaataat
     1021 cccaacgcag gagaaatgtt agctctcaat ggaaatctaa cattagcacg agcagcagcg
     1081 gaaggtaata tagagattgt aaatagacta ttagcttatc ccgaagtaga acaaagcgca
     1141 gcaatcaaca ataatgcaat actgcgtgcc gctcaagcaa acaatcatgt tgctgtggtg
     1201 gagcgactaa tgcaaatccc agcagttaga aacattccaa taccagaatt agggttaatg
     1261 caagtagcgc aatttaatga aaatgctatg cagaccctag atgacaatca acagggtatg
     1321 atcacaatcg ttaaacagca ttatcagcaa aaaatgcaaa gtattggaca agacgcagtt
     1381 cttgaaaatt taaaaaaata tcttgaaacc caatatgaca ataaaccagc tcaggatccc
     1441 tcagggatca aacttccttt ggagcctttt aatccacacc ttccctcaca agagccgtat
     1501 tataaaaaca tttaccatac ggcgcgtcga tatttatttt tgcgtccaaa cccgtggata
     1561 tttccaaatg ctaactacac cgatgaactt cctgatggta gagcggctag tatacccccc
     1621 atggctaaaa ccgatatcgc ctatttttgg ttggcagcgt ctgatacatc atatttacct
     1681 actgcagagt ttacgcataa aaaagttatg aaattattta cacgaagact tgctgacatc
     1741 ggacgtgccc ataattggga caagtatcgg gtgaatgccg ctggtaagtt agaacaatat
     1801 gatgatctag agggagacaa accttcgtgc gatgtcggaa ttaatcagcg tttagtacag
     1861 tccgtgatgg gaaatcccat tacccaacag ccacaagcgc gtgagctaaa tcctagtatt
     1921 tttagaaata aatttataga aaatctttta tttcagcatg aagattatac ggacaatttg
     1981 tatgcgcgtt taaatcaact aagtttgcaa ctattaagcg aattaaaaga agctctcgaa
     2041 aatgaggttg ttttgtgcga acccttaacc gatgcgcaag aagcctgttt ttatatacca
     2101 gaagaacaaa tagatgcttt tatagaagaa gcaaaaacat ggtttagcgc caatcgtatt
     2161 acagcacctt tacaaaccgt aaaatggctg acaagaaatt acgaaaatta tgttgaattt
     2221 gccaatgatt tgtcgaatga tgtaaaaaaa cattttcata atgaagtagt ttccatccta
     2281 gagacactta ttcttcaaaa aacccctatc tcagaaccag aaatcatcaa gactactgat
     2341 gtcgaggcag actcagctga tgatatggaa gctgacaaac ctcgcaaacg ccgccgacta
     2401 taggataact ataaggacac ccacgatact gatgtataaa atgggtgtct ttataaggtt
     2461 caaatagatt tataatgggt gtccttataa atcttctata ccttgttatt tagttaatat
     2521 tccaaatcct ccctttataa tgttttaatg aagtcatttt cgtgctatta aacacgaacg
     2581 acatatctta tttaattagc aagaaaacta attaaattct tgtttcaatt tctttcgaaa
     2641 aaaaatttgc attttttttt aaaatttgtg tacatccccg tttattatgc taagctatgt
     2701 ttgaacatac gcgaaggtgt ggtttatgct gcttactttt ttaagtggcc tttgcagtag
     2761 ttagtagtaa tttgaacaat tcgtttgtag aattttttat atatagtgca gttgcaatac
     2821 tgagttgttt tgagtaatca ttgaggcatt taaaaaggga gaacttgagt gcactgcagc
     2881 agtaaaagtc acccttttat aagttaactt ttatttattt atgacttgat aaggaatggg
     2941 ttcatgaaga aagcactcaa atatacatta tctgcgttgg ctgcagctat gatcgctgct
     3001 cccgcacaag ctaatccgtg gaattttatt aatgagctta cttttaaagc agctctaacg
     3061 tataccgaag cttccggtaa tactgcaaat tacgcgtaca caggcttccc gcaaacttgg
     3121 cgtccgcttt ttgtagagcc agatcacgaa tgggattatg cccttggtct agagtggaga
     3181 attcctggta gcaacaccca tgtgttcttt gattttgaac actttagaga cgatgccagt
     3241 gaaaccgctg ttggtgctgg tacatttggc gcacttcctg ttgcaggtgc tacagcgttt
     3301 ggttctttag aacatcgtga aaaagatttc aaacttggtt tacgccacac gttagaattt
     3361 ggaccgcact ttgacttagc attgggtgct gccatagatg ctgccaaaat tgaaagaagg
     3421 ttcaatgaaa taggtacaga tccagtggtg ggagtcggta caagatttac tcatgaagaa
     3481 accgatgcaa taggtccatt cttcgatgtc acagggcgcg cttacccttg gggtcaaggt
     3541 cgttatggca actgttggag tttatgggca cgggcaggat ttggtttact gtatgcagaa
     3601 catgactaca cgttaacaac tgctgtacca ggattagtac tacctagttt tgatcctgaa
     3661 gatacaaact cgattattac taaagtagaa gcagactttg gggttgaatt tggtcgagtt
     3721 ttgagacttg attttattaa tatgtctctt gctgctagat taggtgttaa atatattaat
     3781 tatatc
//
LOCUS       DASTIB010000105         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_143359, whole genome shotgun sequence.
ACCESSION   DASTIB010000105 DASTIB010000000
VERSION     DASTIB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143359"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFP93_01800"
     CDS             <1..225
                     /locus_tag="VFP93_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HET9843377.1"
                     /translation="DLMIHQWERAHALMKQGQDLGKRLSGLFGLDIRLIIAGGQCVLK
                     KLKSRSDVYERPILQWYDWFRIACKGLYKI"
     gene            368..973
                     /locus_tag="VFP93_01805"
     CDS             368..973
                     /locus_tag="VFP93_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843378.1"
                     /translation="MAISMTWSVCSYPHAEYDEETYYHIKLQAKWRKIRMQELQRQLQ
                     AHTGCNFILTTNACTDYAQGTIYIPENILLLPIEVFTAFISYEWALRMLEKQQSSISP
                     RDAVHKSLQLVGLFLAKNNYDIDHVIKFCLKNNQIPKELNPFSKHLISLMVAFKKALL
                     LRKKAFNPRPNPTRCTTRLKSRGKFLFSRKVLFIAPSYARI"
     gene            991..1275
                     /locus_tag="VFP93_01810"
     CDS             991..1275
                     /locus_tag="VFP93_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843379.1"
                     /translation="MFVKFDVDEKEKLMRTLCEQEILHISGANPVYFMEPENYSSYPL
                     RVYLKRDALLGALGLGLLCSPAGLVVGSLGAIAGLCLGLGCGYLEWVVYQ"
     gene            1365..2213
                     /gene="hpnD"
                     /locus_tag="VFP93_01815"
     CDS             1365..2213
                     /gene="hpnD"
                     /locus_tag="VFP93_01815"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET9843380.1"
                     /translation="MDPQTYCNHKAYQSGSSFYYSFLFLPKPQKEAMLALYAYCREID
                     DVVDEIKDPLVASKTLSWWHDETERLFQGSPQHPVTKALFVHLQSVAWPKSLFYDLLT
                     GMSTDLQDPYFETFQDLERYCYCVAGSVGILSTYVFSFQNPSTLEYAKHLGTCLQLIN
                     IVRDLGEDLRRNRVYLPEEDLLHFKLSRHKLLNLEYSAKDLQPLLTLQANRAKNFFEL
                     AMRHLSSHDRKHQKAGLIMANIYLKLLQLLEQEKFEVLEKKIKLTPLKKLWIAWQTLR
                     KEKKIL"
     gene            2210..>3503
                     /gene="hpnE"
                     /locus_tag="VFP93_01820"
     CDS             2210..>3503
                     /gene="hpnE"
                     /locus_tag="VFP93_01820"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HET9843381.1"
                     /translation="MKRDKVIIVGGGWAGLSCAVMLAQHKIPSVVLEAAPKLGGRARS
                     ISMHGMQVDNGQHLFLGAYRNVQKVLSTLGVNPDKVFKKEPLAWRILSKDRVIDIQLP
                     NTPAPLHGLFGLMRAKGLSFKDKLSALQFCYFLFKKIPLDTITVLQLLEKYSQTPTLI
                     ENLWRPLCLAALSTPPEDASAQVFSQVLQETFFTRNQATDYWFAKKDLSAILPNPAME
                     YLTLHDATVQTSARVTELIFQDQTCIGVKTAQDTYYGRAVVLATPYWTTSQLLQDVQG
                     GEAIVQQCNLLVSEAISTIYLQYSPEVQLPHSMTGLIGTQSQWVFDRNFANQPGLIAV
                     VITGAGMHLTKTQSVLAQEIHQEIQQFWPQCQHLLNWRVIHEKRAVFRCDLLSQECRP
                     QGKTSIPGLFLAGDYCQTPYPATLEGAATSGLQCAEEIR"
BASE COUNT         1081 a          685 c          689 g         1048 t
ORIGIN      
        1 gatcttatga tacatcagtg ggagcgcgcc catgcgctca tgaagcaagg acaagattta
       61 ggcaagcgat tgtccggttt atttggcttg gatattcgct taattattgc tggtggacaa
      121 tgtgttttaa aaaaacttaa atcgagatcg gatgtttatg aacgccccat actccaatgg
      181 tatgattggt ttaggatcgc gtgcaaaggt ctgtataaaa tataagattt tttcgattta
      241 cgtaaacaca tttacagctt gcatcaacga tcgcaaattt cctacgcata aattcctttt
      301 ttcttcttcc aaaatctatt catgttggtt aacttggata ttattatttt gcaatctgga
      361 tttcatcatg gcaatttcga tgacttggtc tgtgtgctcg tacccacatg ctgaatatga
      421 tgaagaaacg tattatcaca ttaaacttca agccaagtgg cgaaaaatcc gtatgcaaga
      481 gctacaacgc caattgcaag cccataccgg ctgtaatttt atattgacta cgaacgcttg
      541 taccgactat gcacaaggta caatttatat tccggaaaat atattattgt taccgatcga
      601 agtttttacc gccttcatat cttatgaatg ggcgctcaga atgcttgaaa aacaacaaag
      661 ctccattagt ccccgtgatg ctgtgcataa gtccttgcaa ttagtaggtt tatttttagc
      721 aaaaaataat tatgacatcg atcatgtgat aaaattttgc ttaaaaaata accaaattcc
      781 taaagaatta aatccttttt ccaaacattt aataagccta atggtagcat tcaaaaaagc
      841 attattgcta cgcaaaaaag cgtttaatcc tagaccgaac cctacgcggt gtacaacccg
      901 tttaaagtct cgaggtaaat ttctattttc tcgtaaagta ctttttattg cgccatccta
      961 cgcacgaata taattcaaaa taagcaaatt atgtttgtta agtttgatgt tgatgagaag
     1021 gaaaaactta tgcggacact ttgcgaacag gaaatcctac acatttcagg cgcgaacccg
     1081 gtgtatttta tggaaccaga gaattatagt tcgtatccgc taagggttta ccttaaacgc
     1141 gatgctcttt taggagcctt aggtttagga cttttatgtt cgcctgcagg actagtggtt
     1201 ggttcattag gtgccattgc aggtttatgc cttggtttag gatgcggtta tctggaatgg
     1261 gtcgtatatc agtaaaccaa caaagctgca aagcttcctg tgtctatagt ccatgttata
     1321 ctgcgagcca gtttgaaata aaaatatgtt agtttattta ctgcatggat ccacaaactt
     1381 attgcaatca caaagcctac caaagtggtt ccagcttcta ttacagtttt ttatttttgc
     1441 caaagccaca aaaagaagcc atgttagcgc tttatgccta ctgtcgagaa atcgatgatg
     1501 ttgtcgatga gattaaagac cctcttgttg cttctaaaac cttgtcatgg tggcatgacg
     1561 aaaccgagcg cttatttcaa ggctcccccc aacatccagt aacaaaagca ctctttgtgc
     1621 atttacaatc ggttgcatgg ccaaaatcgt tgttttacga tttacttacc ggtatgtcca
     1681 ccgacttgca agatccgtac tttgaaacgt ttcaagattt agaacgttat tgttactgtg
     1741 tggcaggctc cgtcggtata ttgtctacgt atgtatttag tttccaaaat ccaagcacct
     1801 tagaatatgc caaacattta ggaacgtgtt tacaactgat aaatattgtg cgggatttag
     1861 gagaagattt acgacgcaat cgcgtttact tacctgaaga agatttgcta cattttaagt
     1921 tatcgcgaca taaattatta aatttggaat attctgctaa agatttgcaa cctttactta
     1981 ccttacaagc aaatcgagca aaaaattttt ttgaattagc catgcgccat ttaagctctc
     2041 acgatagaaa gcaccaaaaa gcagggctta ttatggcaaa tatttattta aaattgttac
     2101 aactactcga acaagagaaa tttgaggttt tagaaaaaaa aataaaatta acaccgctta
     2161 aaaaactttg gattgcttgg caaactttac gaaaagaaaa aaaaatccta tgaaaagaga
     2221 caaagtcatt attgtaggtg gcggctgggc aggtttatcg tgtgcggtaa tgttagccca
     2281 acataaaata ccctctgttg tgctagaagc agctcccaaa ttaggcgggc gagcgcgcag
     2341 tatttccatg catggtatgc aagtggacaa tgggcaacat ctatttttag gtgcgtatcg
     2401 taatgttcaa aaagttctct ccacactcgg ggtcaatccc gacaaagtgt tcaaaaaaga
     2461 accactggct tggcggattt taagtaaaga tcgcgtaatc gatatacaat tacccaatac
     2521 gcctgcccct ctacatggat tatttggtct tatgcgcgca aaaggattat cgtttaaaga
     2581 taaattaagc gccctgcaat tttgttattt tttattcaag aaaataccgt tagacacgat
     2641 taccgtcttg caactgttag aaaaatattc acaaacacct acgttaatag aaaatttatg
     2701 gcgtcccctg tgtttggcag cattaagcac ccctccggaa gatgccagtg cacaagtttt
     2761 ttctcaagtc ttacaagaaa cgttttttac gcgaaatcaa gcaaccgatt attggtttgc
     2821 aaaaaaagat ttgagcgcta ttcttccaaa tcctgccatg gaatatttaa ccttgcatga
     2881 tgctacggtg caaacctctg cacgcgtaac agaattgatc ttccaagatc aaacgtgtat
     2941 aggggtaaaa accgcgcaag acacgtatta cggcagagcc gtagttcttg caacacccta
     3001 ttggaccacg agtcaattat tacaagatgt acaaggcggt gaagctatcg tacaacaatg
     3061 taatcttttg gtaagtgagg ccataagtac tatttatttg caatattctc ctgaggtcca
     3121 attgccgcat tctatgacag gattgattgg tacccaaagc cagtgggtgt ttgatcgcaa
     3181 ctttgcaaat caaccaggcc ttattgcggt ggtgataaca ggtgccggaa tgcatttaac
     3241 caaaacgcag agtgtgttag cacaagaaat tcatcaagaa atacaacagt tttggccaca
     3301 gtgccaacat ttattgaatt ggcgcgtgat ccatgaaaaa cgggcagtat ttcgctgtga
     3361 tctgttatcc caagaatgca ggccgcaggg taaaacttca atccctggat tatttttagc
     3421 aggggattat tgtcaaacac cctatcctgc gactctggaa ggagcggcta ccagtggttt
     3481 acaatgtgcg gaagaaatac gaa
//
LOCUS       DASTIB010000106         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_903249, whole genome shotgun sequence.
ACCESSION   DASTIB010000106 DASTIB010000000
VERSION     DASTIB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903249"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..691)
                     /gene="rpiA"
                     /locus_tag="VFP93_01825"
     CDS             complement(38..691)
                     /gene="rpiA"
                     /locus_tag="VFP93_01825"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET9843382.1"
                     /translation="MNDTLKQKAAQAVIEAIELDAILGVGTGSTIGFFIEELGKLKHK
                     LLGVVASSVDTENKLKKLQIPLVSINAGPIAFYVDSADECNEHRQLIKGGGGALTREK
                     IIAAVAEKFICIIDDSKLVKVLGTYPLPIEVIPMARSYVARELVKLGGDPVYRENFVT
                     DNGNQILDVYNLNLTNPTHMEATINNITGVVCCGLFARKPADRVLIASQNGIKYLNP"
     gene            complement(701..1168)
                     /locus_tag="VFP93_01830"
     CDS             complement(701..1168)
                     /locus_tag="VFP93_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET9843383.1"
                     /translation="METHYWLFKSEPDTFSIDDLTIKPNRTDSWEGVRNYTARNLLRN
                     SIQVGDLGFFYHSSCKNPGIVGIIEVTRAGYPDPTALNPKSKYYDSKSTIEKPRWYCV
                     DVRFKEKFKEPIFLQALRNNPALKDFTLLKKGNRLSVLPVTEKEWNTILLMQT"
     gene            complement(1209..1802)
                     /locus_tag="VFP93_01835"
     CDS             complement(1209..1802)
                     /locus_tag="VFP93_01835"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET9843384.1"
                     /translation="MNAIPELKTQLRQEMLQLRNQLTSEEIRTYSLELHSVVKNCPML
                     VHANRIGFYFSHQKEIATTFLLEEALRQHKKIFLPALQNTTTSLVFLRKREGTKLIPN
                     KYGIMEPDPCATLHISAQRLEVVFVPLVAFDAFGNRLGMGGGYYDKTFAFKKLGKQQP
                     ILIGLAYDFQQVDTVPHDTYDIKLDYVITPSTIMHFG"
     gene            complement(1813..1989)
                     /gene="ssrS"
                     /locus_tag="VFP93_01840"
     ncRNA           complement(1813..1989)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFP93_01840"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2003..2407)
                     /locus_tag="VFP93_01845"
     CDS             complement(2003..2407)
                     /locus_tag="VFP93_01845"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET9843385.1"
                     /translation="MSDNDKGFKVTIVDKEFRVNCTKDAENALQEAATYLDKQMRKIR
                     NTGRVIGLERIAVMAALNICHELLTLKSKSPEEEVKNLSDRLQVLQNKIDGALSDKPT
                     IYDKLNFKHTLQNEFEKFETTHRSNKEILEYE"
     gene            2567..3133
                     /locus_tag="VFP93_01850"
     CDS             2567..3133
                     /locus_tag="VFP93_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HET9843386.1"
                     /translation="MSHVTSVFDYNHWVERFQALKKDLMPIELQGLAMAFVCLIQESL
                     VKSQWLKFLQEEYQHTVEEENYPSFVQMTEELLEFCIANFKTDFNLQLCLPSDETPLP
                     VRVKALSDWCHGFIYGLAVKTDPAYMKAKEMQDILRDLCEITKVTLETCDEEEEKAYT
                     ELVEYVRLIPFVISEVVKSLQHSTPTIH"
     gene            3143..>3465
                     /locus_tag="VFP93_01855"
     CDS             3143..>3465
                     /locus_tag="VFP93_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET9843387.1"
                     /translation="MESHQNVKAYIDRRLKILAQMSPNSACFIPANKKILRNGDAEYP
                     FRQNSSLYHVTGWPEDLALLVLTKKNNQHKTIFFCHEQDAMTSRWVGERLGTLRARQE
                     YGMEEA"
BASE COUNT         1016 a          665 c          646 g         1138 t
ORIGIN      
        1 ggtgtagatg aaaccccctt gccggatcct ttagcaccta aggatttaga tatttaatgc
       61 cattttggga ggcgataagc acgcgatcgg cgggtttgcg cgcaaataag ccgcagcata
      121 ctacaccggt gatattatta atagttgctt ccatgtgtgt tggattagtt agatttaaat
      181 tataaacatc caaaatttga ttaccattgt cggttacaaa gttttcgcga tataccggat
      241 ctcctcctaa tttcaccagt tctcttgcga cataactgcg ggccattggg ataacttcaa
      301 taggtaatgg ataagttccc agcaccttga ccaatttgct atcgtctatg atacaaataa
      361 atttttcagc aactgcggca ataatttttt ctcgcgttag cgcaccaccg cctcctttaa
      421 ttaattgacg gtgttcattg cattcgtccg cgctatccac ataaaatgca ataggacccg
      481 catttatcga tacaagtgga atttgcagct tttttaattt gttttctgta tccaccgagc
      541 ttgctactac accaagcaat ttatgtttaa gcttacctaa ttcttcaata aaaaatccta
      601 tggtggatcc agttccaaca cctagaatag catcaagctc tatagcttcg attacagctt
      661 gtgcggcctt ttgctttaat gtatcgttca tgcccagccc ttaggtttgc attagtaata
      721 ttgtgttcca ttctttttct gttacaggta atacagagag gcgattccct tttttaagca
      781 aggtaaaatc tttaagagcg gggttattgc gtaatgcttg taagaaaata ggctctttaa
      841 atttttcttt aaaacgcaca tcaacacaat accacctagg cttttcgatt gtgcttttac
      901 tatcgtaata tttagactta ggattcaatg ccgtaggatc gggatacccg gctcgggtca
      961 cttctatgat gcctactata cctggatttt tgcaactgga atgataaaaa aatccaagat
     1021 cacctacttg aatagaattg cgcaacaaat tgcgcgccgt ataattgcgc acaccttccc
     1081 agctgtcagt ccgattaggt ttaatggtta aatcatcaat gctaaaagta tcgggttctg
     1141 acttaaataa ccaataatgt gtttccattc gatgtttttt aaatacttgt aacggtttag
     1201 ctgaaatttt atccgaaatg cataattgta gatggagtta taacataatc taattttata
     1261 tcatatgtgt catgaggaac ggtatccact tgttgaaaat cataggccag ccctatcaaa
     1321 atcggttgtt gtttacccaa cttcttaaag gcgaaagttt tgtcataata gccaccaccc
     1381 atacctagac gattgccaaa cgcatcaaac gccactaaag gtacaaaaac tacttccaaa
     1441 cgttgggctg atatatgaag cgtggcacac ggatctggtt ccattatacc atatttgtta
     1501 ggtattaatt tagtcccctc tctttttctt aaaaaaacca aggaagtagt agtattttgg
     1561 agagcaggta agaatatttt cttgtgctgc cttaatgcct cttccaataa aaaagttgtt
     1621 gcaatttctt tttgatggga aaaataaaaa ccgatacgat tggcatgcac aagcattgga
     1681 caatttttta caacactgtg taattccaaa gaataggtgc gtatttcttc tgaggttaat
     1741 tgatttctta actgcagcat ttcttgtcga agctgcgttt ttaattcagg tattgcattc
     1801 atgaaatagg tggtgttccc taaacagccc gcacaatatc cttgaaccct ttggttcaag
     1861 tgggcacctc agcctcgcat caggctttcc acattgagtg gacttgcaca acaacaagga
     1921 ctttctggct ccctttctgg tagcatcggc tcaagatgta taatgcggag taatgctgcc
     1981 aagagaacac cgaaaacgga actcactcat attcaaggat ttctttatta cttctatgag
     2041 tagtttcaaa tttttcaaat tcattttgta aggtgtgctt aaagtttagt ttatcataaa
     2101 tcgtgggttt atctgaaagc gccccgtcaa ttttattttg taatacttgt aagcgatcac
     2161 ttaaattttt tacttcttct tctggagatt tactttttaa tgttaataat tcatggcaaa
     2221 tattcaaggc tgccatgaca gcaatacgct ctaagccgat aacccgccca gtattgcgaa
     2281 tttttctcat ttgtttgtct aaataagtag cagcttcttg taatgcattt tctgcatctt
     2341 tggtgcaatt cacgcgaaat tctttatcta ctatggttac tttaaatcct ttgtcgttat
     2401 cactcatgca ggctcctatt tttgcgaaca gttaagggaa ctttcgaaat ataagaagtt
     2461 tacgtggttc acgcattttg ttccactttt ataatgtcaa gcgcgttgct aagtatttat
     2521 tttattatgt tactattact cctgcagtta taaggagtat aatattatga gtcatgtaac
     2581 tagtgttttt gattataatc attgggttga acgttttcaa gcgttaaaaa aagatttgat
     2641 gcctattgaa ctacaaggcc ttgcgatggc atttgtatgt ctaatccagg aatctttagt
     2701 aaaaagtcaa tggttaaaat ttttgcaaga agaataccag cacacagtgg aagaagaaaa
     2761 ttatccctcc tttgttcaga tgaccgaaga attactagaa ttttgtattg ctaactttaa
     2821 aaccgatttt aatttacaat tatgtttgcc aagtgatgaa acaccactcc cggtaagagt
     2881 aaaagcgtta agcgattggt gtcatggttt tatctatggt ttagccgtaa aaacggaccc
     2941 ggcctatatg aaagccaaag aaatgcaaga cattttgcgt gatttgtgtg aaataacaaa
     3001 agttacgctg gaaacctgtg atgaggaaga ggaaaaagct tatacggaat tggtagaata
     3061 tgtccgactt attccatttg ttatttctga agtcgtcaaa tctttacagc attctacgcc
     3121 aactattcac taaggatgtt ctatggaatc gcaccaaaat gtaaaggctt atatcgatag
     3181 acgtctcaaa attttggcac aaatgtctcc caatagcgca tgctttattc ctgctaataa
     3241 aaaaattctg cgcaatggag atgcggagta tccttttcgg caaaatagct ctctatatca
     3301 cgttaccggt tggcctgaag atcttgcttt acttgtttta acaaaaaaaa ataatcaaca
     3361 taagacaatc tttttttgtc atgaacaaga tgctatgact tcacgctggg tgggtgagcg
     3421 cttagggact ttacgtgcgc gccaagaata cgggatggaa gaagc
//
LOCUS       DASTIB010000107         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_50756, whole genome shotgun sequence.
ACCESSION   DASTIB010000107 DASTIB010000000
VERSION     DASTIB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50756"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /gene="cysS"
                     /locus_tag="VFP93_01860"
     CDS             <1..986
                     /gene="cysS"
                     /locus_tag="VFP93_01860"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9843388.1"
                     /translation="GYAYLADNGDVYFQVEKFASYGELSHRDLENLQAGARVEVNEVK
                     KNPLDFVLWKKSKKNEPSYPSPWGEGRPGWHIECSAMSTHCLGKHFDIHGGGHDLIFP
                     HHENERAQSEGAFEQTFVNTWMHVGFVQVNKEKMSKSLNNFFTIREILQNYSGEVLRY
                     FLLSSHYRSPLQYGPDLLENAKSGLSTLYMAIRGVTISHTDTTLLKNHPIVSQFEAVM
                     NDDFNTPKAISLLFELAHTINTRKQKGESSEKEAAVLKYLGSILGLLQQESEQFLQGE
                     SLDFDVEAMIAARNQARLEKNWAKADEIRNFLLEKGIVLEDTATTTLWRRN"
     gene            complement(1266..1946)
                     /locus_tag="VFP93_01865"
     CDS             complement(1266..1946)
                     /locus_tag="VFP93_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET9843389.1"
                     /translation="MLINKIPYFLLLCVIGVAEGATYKWKDQNGQTVYGASPPPGVQA
                     ERIHTAPPSSSSDEEIKAFQQRQQQELESAKKAEFEANIAKEKEQEQKIWQVNCNAAK
                     NKLLSLKEKPRVRQPDSSGQLAVIPEEEVALKIKQAETEVQVYCNRSKESSMKPSNTQ
                     DQPQKLEQIPTSDTTDINENNTNATGTTSPTGAPLETTTGPQPRVNTSPTSLEVDPND
                     TVPETPTP"
     gene            complement(1933..2226)
                     /locus_tag="VFP93_01870"
     CDS             complement(1933..2226)
                     /locus_tag="VFP93_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016210632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HET9843390.1"
                     /translation="MTEKKKGSKIIIEGVTEDGVTFRPSDWAERMSGSLSTFKNHRIY
                     YSPLLRPSFRGGNRCVILDESLKESNPELYEHILDFARRNKLKICGEDEDVDK"
     gene            2412..>2650
                     /locus_tag="VFP93_01875"
     CDS             2412..>2650
                     /locus_tag="VFP93_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9843391.1"
                     /translation="MAYKHTDTEKIERSTRRGIYLLPNLLTTTGLFAGFYAIVAAMRG
                     EFDNSAIAIFIAMIMDGLDGRVARLTNTQSAFGVE"
BASE COUNT          746 a          495 c          572 g          837 t
ORIGIN      
        1 acggctatgc gtatttggcg gataatggtg atgtatattt tcaagtagaa aaatttgcat
       61 cttacggtga gctctcccat cgtgatttag aaaatttgca agccggtgcg cgcgtagaag
      121 tgaatgaagt caaaaaaaat ccgctcgatt ttgttttgtg gaaaaaatca aaaaaaaatg
      181 agccaagtta cccttcgcct tggggagaag gacgtccggg atggcatatt gaatgttccg
      241 ccatgtcaac ccattgtttg ggaaaacatt ttgatattca tggaggcggg cacgatctca
      301 tttttcccca tcacgagaat gagcgtgcgc aatctgaggg tgcatttgag caaacgttcg
      361 tcaacacgtg gatgcatgtg ggttttgtcc aagtaaataa agaaaaaatg tccaagtctt
      421 tgaacaattt ctttacgatt cgcgaaattt tgcaaaatta ttccggggaa gttttgcgct
      481 attttctgtt atcttcccac tatcgaagcc cactgcaata tggccccgat ttattggaaa
      541 atgctaaaag tggcttaagt actctctata tggcaattcg tggcgtaacc atatcccata
      601 ctgatactac tttgttaaaa aatcatccca tagtaagtca attcgaagct gtgatgaacg
      661 atgattttaa cacccctaag gccataagtt tgttatttga actggcgcat accattaata
      721 cgcgcaaaca aaaaggcgaa tcttcggaga aagaagcggc tgttttaaaa tatttaggtt
      781 ctatcttagg tttgctacaa caagagagtg aacaattttt acaaggtgaa tctttagatt
      841 ttgatgtaga ggctatgatc gcagcacgca atcaagcacg tctagaaaaa aattgggcaa
      901 aagcggatga aataagaaat ttcttgttgg aaaaaggtat tgttcttgag gataccgcaa
      961 ccactacgtt atggcgcagg aattaacagc tgagcaaatg tgggtatcac ggcgaatgcg
     1021 gtaatctctg ttattaccaa gaatgcggta aatctctgtc attaccgcaa atgcgttaaa
     1081 tctctgtcat tacggcgaat gtggtaaatc tgtcattacc gcgaatgcgg taaatctgtc
     1141 attacggcga atgtggtaaa tctgtccctt accgcgaatg cggtaaatct gtcattaccg
     1201 cgaatgcggt aatccatctt actcttacac aagataaacc caagcacaat cgacacgata
     1261 ctaaattaag gtgtcggtgt ttccggaacc gtatcgttcg gatccacttc taaagatgta
     1321 ggagaagtat taacgcgagg ctgtggtccg gttgtggtct ctaagggagc gccggtagga
     1381 gaagttgtgc ctgttgcatt tgtgttattt tcattgatat cagttgtatc tgaagtggga
     1441 atttgttcaa gtttttgtgg ctgatcttgg gtattggaag gtttcattga cgattctttc
     1501 gagcgattac aatacacttg cacttcggtc tctgcttgtt taattttcaa cgccacttct
     1561 tcttcaggaa taaccgcaag ttggccagaa ctatccggtt ggcgtaccct tggtttttcc
     1621 tttaatgaca ataatttatt tttggcagca ttacaattta cttgccaaat tttttgttct
     1681 tgttcttttt ctttagcaat attggcttcg aattcggctt ttttggcgga ttctagctct
     1741 tgttgttgac gttgttggaa agcttttatt tcttcatcgg aagaactgga gggtggagca
     1801 gtatgaatgc gttctgcttg aaccccagga ggaggggagg caccatatac agtttggcca
     1861 ttttgatctt tccacttata ggtggcacct tcagcgacgc ctattacaca taacaacaga
     1921 aaataaggta ttttatttat caacatcctc gtcttctccg catattttca gcttatttct
     1981 tcgggcaaaa tcaagtatat gctcgtataa ttcaggatta ctctctttta aactttcatc
     2041 taagattaca catcgattcc ctccgcgaaa ggagggtcgc aataaagggg aatagtaaat
     2101 acgatgattt ttaaaggttg ataaactgcc actcattctt tccgcccaat cgctagggcg
     2161 gaaggttacg ccatcttcgg ttactccctc gattataatt ttggaacctt tttttttctc
     2221 agtcatgggg aacatttcaa aatgatgata gttggaataa taacatagtt gggcctgatc
     2281 agaatatatc cttgagatta taaataatag tataacgtaa aatactattt tcggaccttt
     2341 ttcggtctta ttcggacatt ctgctattat gagtaaattt cgttctaaaa ttcaattaaa
     2401 ttaaataact tatggcttat aaacacactg ataccgaaaa aatagaaaga agtacacgac
     2461 gcgggattta tttgcttccc aatctgctta cgacaacggg gttatttgca gggttttacg
     2521 cgattgttgc tgcaatgcgt ggggaatttg ataattctgc gattgccatt tttattgcca
     2581 tgatcatgga tggcttagat ggtagggtag cgcgccttac taatacccaa agtgcattcg
     2641 gggtagaata
//
LOCUS       DASTIB010000108         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1061123, whole genome shotgun sequence.
ACCESSION   DASTIB010000108 DASTIB010000000
VERSION     DASTIB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061123"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            751..2916
                     /locus_tag="VFP93_01880"
     CDS             751..2916
                     /locus_tag="VFP93_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843392.1"
                     /translation="MLIYSGKDGKKLREIGSKLKEDSTGALLDEHQNRFEIKQKLGGR
                     GQAGLANFYSPDGGKTQYLIKQDDAATCVLEGTAYFLRDTRILPPSLSGAVNYAKLGS
                     LISNNDETLIVSIQDRVMPDVIGGKIQPWDELVFGAKRNPHTWVSQEALHTFWNKKGI
                     KTPVYSLSSSAQWDLAAGILASAIVGDESLHVGQFMAKLDTEGTITGITRIDLGARER
                     FAVSRSENKNLDPFHASPEYQSKGQFNKDYVSFLLEEPTLRQKYSQLWARLHANKDAV
                     ANNVYEKSRQAIIEQFQILPNDKIKKEALENILKAINKAGKTSVNDPVKVDPEAPVDK
                     QIETLANVMAKLDAERTHAMIVAGYKEFTTQLDADKQKALELRLEEEGRNLLGAVRQF
                     RQDLILGNGKNEEQILGELYNHLKKQIALSLQKFEKNPSNSLYPQQIQFMAEQALNLI
                     ESYKLHEMDKNNPNRNVIKKLEERVDRFKTMHDVAKYCVRTEDKAKQAHSRGIIGAAL
                     TMERQDFRKWLEDEEKRLIQVLGAHSGLGGYMGKPTHGLQLLQNLYAHYNLPSASLVM
                     NKTQIELLEASVSKDFSKVFHITQSQNFTVDDALVRDRYGRTVLHYLMDHGSGNEASA
                     AIVNILKNSFGNLSNTNLNIQDNNGDTPAHLLMGNPNSQEIVSNIDKAKLKATTFGGS
                     YKFNDFFKMDSPNKAGLTPKDILDRQSIEAQPNKNRPYK"
BASE COUNT         1069 a          490 c          591 g          856 t
ORIGIN      
        1 ttgcaaaaat gcataaataa taaaaaattt actttgaaaa cattgaaaat gtatattcct
       61 gacacttgtt ttcgatgtta atgtttcgga atggactttt tccgagtctc gaacgatgga
      121 atggtaagat gcaaaaattt aagagacttt ttttcacaat aataatgagc ctcaaccttt
      181 ataactgttg ggaatcagga tgaaccgaat atggacacaa aaaaggttta gactatatcg
      241 aagaaactat gaagttttat aaaaataatc aattggcaaa tgatcattcc atgataaaga
      301 ttttggaaaa taattcaacc gttaaaaata gagttaataa tataaataga ttttgcagtc
      361 gattgtgaaa tcgtcaaaac tgtttgatgt cttttaaagt cttttaaatc taatgtttat
      421 tactttcaat tggttcacag ttcttagcaa ttacacctca caaacacacg ttgcgcgagt
      481 tgttcgcgca accgcctatt catactattt atggtatttt tatttataaa tgtagtaata
      541 gttgaatttt gttaggaatg ttattacttt tagcggcctc agatgaccgc tcatcgcaag
      601 cgcatacaca aaaaatagca atcccataca atatacaaaa cgataatttt taatgatttg
      661 cttaaagtca gatgaacgaa cttgtcccaa taaaaaatag gtcacaaagt aagtgaaatt
      721 taaagaagat aattgattga gggtttataa atgttaattt actcaggtaa agatgggaag
      781 aagctgcgag aaattggttc aaaattaaaa gaagattcca ccggagcttt actagatgaa
      841 catcagaatc gctttgaaat taaacaaaaa cttgggggta gagggcaagc aggtttggcc
      901 aatttttaca gcccggatgg tgggaaaaca caatatttaa taaaacaaga tgacgcagcg
      961 acctgtgtct tagaaggcac agcctatttt ttgagagata cacgaatttt accccccagt
     1021 ctgagtgggg ctgttaatta tgccaaatta gggtcattga ttagcaataa tgatgaaaca
     1081 ctgatcgttt caattcaaga tcgtgtgatg cctgatgtaa ttggcggaaa aattcaacct
     1141 tgggatgaac tggtttttgg tgcaaaaaga aatccccata cttgggtaag tcaagaagcg
     1201 ttgcatactt tttggaataa aaaaggtata aaaactccgg tgtattcatt aagttcaagt
     1261 gcgcagtggg atctcgccgc aggtatactt gcaagtgcaa tagtggggga tgaaagttta
     1321 catgttggac aattcatggc taaattagat accgagggaa cgattacggg catcacgcgt
     1381 attgatttag gtgcgcgtga acgttttgca gtttctagaa gtgagaataa aaatctagat
     1441 ccttttcatg cctcaccgga ataccaatct aaaggccaat ttaataaaga ttatgtgagc
     1501 tttttattag aagagccaac attgcgccaa aaatattcac aactatgggc acgcttacat
     1561 gctaataaag atgctgtggc aaataatgtt tatgaaaaga gtaggcaggc aatcattgaa
     1621 caatttcaaa tactgcctaa tgataaaatt aaaaaggaag ctttagagaa tattttgaaa
     1681 gctattaata aagcaggcaa aacttcggtg aacgaccctg tgaaagtaga tccagaagca
     1741 cctgtcgata aacaaattga aacgctcgct aatgttatgg cgaaattgga tgctgagcgt
     1801 acgcacgcta tgattgttgc aggctacaaa gaatttacta cacaattaga tgccgataaa
     1861 cagaaagcat tggaattaag attagaggaa gaaggaagaa atttattagg tgctgttcgt
     1921 caatttagac aggatcttat attggggaat ggtaaaaacg aagaacaaat acttggggaa
     1981 ttatataacc atcttaaaaa acaaattgct ctaagtctac aaaaatttga gaaaaatccg
     2041 agtaattccc tataccccca acaaatacaa tttatggcag aacaggccct gaatttgatt
     2101 gaatcttata aattacacga aatggataaa aataatccga ataggaacgt aattaaaaaa
     2161 cttgaagagc gagttgatcg ctttaaaacc atgcatgacg tggcaaaata ttgtgtgcgt
     2221 accgaagata aagctaaaca agcgcattct cgaggaataa tcggggcagc tttaactatg
     2281 gagcgacaag attttcgtaa atggcttgaa gacgaagaga aacgcttgat tcaagtgtta
     2341 ggtgcgcatt cgggactcgg aggctatatg ggtaagccga ctcatggttt gcaattatta
     2401 caaaatcttt atgcacatta caacttacct tcagcaagtt tggttatgaa taaaacccaa
     2461 atagaactac tcgaagcatc agtgagcaaa gatttttcga aggtttttca tataacccaa
     2521 agtcaaaatt ttaccgtcga tgatgcccta gtacgagata gatatggtcg cacggtattg
     2581 cattatttaa tggatcatgg aagtggcaat gaagcttcgg cagctattgt aaatattcta
     2641 aaaaactcat ttggcaatct atccaacact aatttaaata ttcaagataa taatggagat
     2701 acgcctgcgc atttactaat gggtaatcct aactctcaag aaattgtatc aaatattgat
     2761 aaagctaagc tgaaagcaac cacattcggc ggaagctata aatttaatga ttttttcaaa
     2821 atggattcac ccaataaagc aggcctcact cctaaagata tcctagacag gcaaagcata
     2881 gaagctcaac ctaacaagaa tcgaccgtac aaataagctg ctttgcccgt atgtacggca
     2941 tagcagctac gggcttattt actttacatt aagttttgtt gcgggttgca tactctcaac
     3001 ttctct
//
LOCUS       DASTIB010000109         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1135774, whole genome shotgun sequence.
ACCESSION   DASTIB010000109 DASTIB010000000
VERSION     DASTIB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135774"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFP93_01885"
     CDS             <1..694
                     /locus_tag="VFP93_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9843393.1"
                     /translation="VFYQAMKSIRTGLILGTVTTLFMLPIAIVLGIVAGYFRGLADDI
                     IQYLYTTLSSIPGVLLIAAAVLILQVWMTQHDAQFQTTLERSDARLLALCVILGVTSW
                     TGLCRLLRAETLKLREIEFVQAAKALGVKNFKILFKHLLPNMMHIILISVALDFSGLV
                     LAEAVLSYVGVGVDPASYSFGTMINSARLEMARLPVVWWSLSAAFIFMFTLVLAANIF
                     ADAIRDAFDPRL"
     gene            710..2572
                     /locus_tag="VFP93_01890"
     CDS             710..2572
                     /locus_tag="VFP93_01890"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9843394.1"
                     /translation="MDEYILKINQLSIGIRHNSEILYAVDDLSFNLPRGKTFALVGES
                     GCGKSITAHSIIRLLPEKAIIYQGSDIVLDDYPLLTYSEFHMREIRKKIGFIFQDPMA
                     TLNPVLTVRTQLEEALYDPPKNPIEKLIHILEAVRIVEPQRVLDAYPHELSGGMKQRV
                     MIAMAVLKEPILLIADEPTTALDVTTQAQVLKILGELQRTNNMSILLITHDLGVVAQL
                     ADEVAVMYAGHIVEHANVKDFFHHPHHPYSQKLLAAIPENSDKRRRLSVIPGRVPQLT
                     ERDPLCRFRARCHNVLPICHEIPAINFAVNGYGEVRCHGYNKNLQPEERPKIELPEPP
                     PLLEAYQTSKEPILQVENLKVYYPIKSSFLKRTIGFVKAVDGVSFALSAGQTLALVGE
                     SGCGKTTTGKAILKLIESTEGKVLYWGKDIRCMPERKLRKVRGDLQFIFQDAFAAMDP
                     KMRIHQILEEGMIALKVGSDKKERQERIDNLLSQVGLSPQVKFRYPHEFSGGQRQRIA
                     IARALAVSPKIIICDEPTSSLDVSVQAQILNLLDTLKKEYEISYIFITHNIGIVRYLA
                     DVVAVMHMGKIVEIGEADSILNQPQHPYTKTLLDSVPSIHNIENVLSQSLIRES"
     gene            complement(2555..>3174)
                     /locus_tag="VFP93_01895"
     CDS             complement(2555..>3174)
                     /locus_tag="VFP93_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843395.1"
                     /translation="TYSRYLDKIPLGVYLKTMHTLFHWHGANALDNLYTLGILGSAFF
                     LHDINYDLSLQPKMYNFWHYKLSKAILDPGNLVGLILILPLTNRLLHDSFKNALDDVI
                     EEFLNHWNHDDEASKHHLACILKSVLPDYLKQYQLYQVPNLSNNFSQLNTCIHNSIDI
                     ADYEEIPNISSTSFIPHYTKQTQTLNTISMGDQLQTKSVQSRFTN"
BASE COUNT          970 a          558 c          651 g          995 t
ORIGIN      
        1 tgttttttac caagcgatga aaagcattcg aaccggcttg attttgggca ctgtaactac
       61 attattcatg ctacctattg ccattgtctt gggcatagtt gcaggatatt ttcgaggatt
      121 ggctgatgat attatccaat atttatatac caccctaagc tccattcctg gtgttttgct
      181 gattgctgca gcggtattaa ttctacaagt atggatgacg caacatgatg cacaatttca
      241 aactacctta gagcgatccg atgccagatt acttgcgtta tgtgtgattt tgggggtaac
      301 cagttggaca ggattatgta ggctgttgcg agccgaaact ttaaaactaa gagaaattga
      361 atttgttcaa gcagccaaag ctttgggtgt gaaaaatttt aaaattttat ttaaacattt
      421 attacctaat atgatgcata ttattttaat ttccgttgcg ctggatttca gtggccttgt
      481 tttggcagag gctgttttat cctatgtagg cgtgggcgtg gatccagcgt cgtatagttt
      541 tggcacaatg ataaatagtg cacgactcga aatggcacgt cttcccgtgg tttggtggtc
      601 attgagtgct gcgtttattt ttatgtttac tttagtattg gctgcaaaca tttttgcgga
      661 tgctattcgc gacgcattcg atccaagatt gtaaggaact tgttaacaag tggatgaata
      721 tatacttaaa attaatcaat tgagcattgg tattcgccat aattcggaaa ttttgtacgc
      781 cgttgatgat ttatctttca atctccccag aggtaaaacg tttgctttag taggagaatc
      841 tggttgtggc aaaagcatta cagcgcatag tattattcgt ttgttacctg agaaagccat
      901 aatatatcaa ggaagcgata tcgtattaga cgattatcct ttgttaacct attctgaatt
      961 tcatatgcgt gaaattagaa aaaaaattgg ttttattttt caagatccca tggccacctt
     1021 aaatcccgtt ttaacggtgc gaacgcagct agaagaagct ctttatgatc ctccaaaaaa
     1081 tccgatcgaa aaactcatcc acatcttaga agcggtacgt atagtagaac cacaacgcgt
     1141 gcttgatgcc tatccgcatg aattatcagg agggatgaag caacgtgtca tgattgctat
     1201 ggcggtatta aaagagccta ttttattaat tgcagatgaa cccaccaccg cactcgacgt
     1261 aacgacacag gcccaagttt taaaaatact gggtgagtta cagcgcacca acaacatgag
     1321 tattttactt attacgcatg atttaggcgt tgtagcgcaa ttggcagacg aagtggccgt
     1381 gatgtatgca ggacatattg tcgaacatgc gaatgtaaaa gatttttttc accatcctca
     1441 ccatccgtat tcacaaaaat tgttagctgc tattccagaa aacagtgata aaaggcgacg
     1501 tttatccgta ataccaggcc gtgttccaca attaaccgaa cgggatcctt tatgccgatt
     1561 tcgtgcacga tgtcataatg tgcttccgat ttgtcatgaa attccagcga ttaattttgc
     1621 tgtcaacgga tatggagaag tgcgatgtca tggttacaat aaaaacctac agccagagga
     1681 aaggccaaaa atagaattgc ctgaacctcc acccttgttg gaagcctacc aaacctcaaa
     1741 agaacctatt ttgcaggtag aaaatctgaa agtttattat cccattaaaa gtagtttttt
     1801 gaaacgcaca attggttttg tgaaagccgt cgatggcgta tcttttgcgc tttctgcagg
     1861 acaaacctta gctttggtag gtgagtccgg gtgtggcaaa actaccacag gtaaagcaat
     1921 tttaaaactt attgaaagca cggaaggcaa agttctttat tggggtaaag atattcgttg
     1981 catgccagaa cgtaaactgc gaaaagtccg tggtgatttg caatttattt ttcaagatgc
     2041 ctttgcagcg atggatccta aaatgcgcat ccatcaaatt ttagaagaag ggatgattgc
     2101 cttaaaagtg ggaagtgata aaaaagaacg gcaagagcgt atcgataatc tactgtccca
     2161 ggttggtttg tcaccgcaag taaaatttcg ctatccgcat gaattttctg gaggacaacg
     2221 acaacggatt gctattgcaa gagcgctcgc agttagcccc aaaattatta tatgcgatga
     2281 acccacaagc tcactcgatg tttcggtaca agcgcaaatt ttgaatcttt tagacacttt
     2341 aaaaaaagaa tatgaaatta gttatatttt tataacgcac aatattggaa ttgtgcgtta
     2401 tttggcagat gttgttgcag tgatgcatat ggggaaaatt gtagaaatag gagaggcaga
     2461 tagtatatta aaccaaccgc aacaccctta caccaaaact ttattagatt cagttccttc
     2521 tattcataat attgagaatg tgttatcaca atctttaatt cgtgaatctt gattgaacac
     2581 ttttagtttg taattgatcc cccatggaaa tggtgttgag cgtttgtgtt tgtttcgtat
     2641 agtgaggaat gaacgacgta gaagaaatgt tggggatctc ttcatagtca gcgatatcaa
     2701 tagaattatg tatgcaagtg ttaagttggg aaaaattatt gcttaaattg ggcacttgat
     2761 ataattgata ttgctttaaa taatctggca gtacactttt taaaatacat gctaagtgat
     2821 gtttgcttgc ttcatcatca tggttccaat ggtttaaaaa ttcttcgata acatcatcta
     2881 aagcattttt aaagctatca tgcagtaacc tattggttaa aggtaaaatt aatattagtc
     2941 ctacgagatt accaggatct aaaatagctt tggataactt ataatgccag aagttataca
     3001 tttttggctg caaacttaaa tcataattta tatcgtgtag aaaaaaggcc gatcctaaaa
     3061 ttcctaaagt gtataaatta tccagtgcat tcgctccatg ccagtgaaat aacgtgtgca
     3121 tagtttttaa ataaactcca agcggaattt tatcaagata gcgactatac gttt
//
LOCUS       DASTIB010000110         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1413733, whole genome shotgun sequence.
ACCESSION   DASTIB010000110 DASTIB010000000
VERSION     DASTIB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413733"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..657
                     /gene="sdhC"
                     /locus_tag="VFP93_01900"
     CDS             280..657
                     /gene="sdhC"
                     /locus_tag="VFP93_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET9843396.1"
                     /translation="MITNRPLHINPFMVRMPIPALVSISHRLSGVAIFLLIPLLLWIL
                     DQSLISPENYETLNTYLQNWWLKPIVFIFILSLFFHLIAGIRHLLMDMHIGESLQGGR
                     ISSWIVFILAFLASLFSATWLWG"
     gene            660..986
                     /gene="sdhD"
                     /locus_tag="VFP93_01905"
     CDS             660..986
                     /gene="sdhD"
                     /locus_tag="VFP93_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HET9843397.1"
                     /translation="MSSRKGFRDWLIQRITAVFLGVYSVIFLLFFLRNPHPTYIDWSN
                     FFYQPIMQICTFIMLISLMWHAWIGLWTIITDYIKPISLRLITLGFVILILFYYFAWI
                     FKILWG"
     gene            990..>2417
                     /gene="sdhA"
                     /locus_tag="VFP93_01910"
     CDS             990..>2417
                     /gene="sdhA"
                     /locus_tag="VFP93_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017493930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET9843398.1"
                     /translation="MANPAVRSFDAVIVGAGGAGMRASLELARAGRKVGLVSKVFPTR
                     SHTVSAQGGITVSLGNQHEDHWQWHMFDTVKGSDYLGDQDCIEYMCQVGPEVIYELEH
                     MGLPFSRMEDGRIYQRPFGGQSKNYGGEQATRTAAAADRTGHALLHTLYQNNLKEDVT
                     IFSEWYALDLVKSQTGRIAGILVLSIETGEVLLLKGKAIILATGGAGQIFHSTTNALI
                     NTGDGIGMVLRAGFCVQDMEMWQFHPTGIAGVGVLVTEGCRGEGGYLVNKDNERFMER
                     YAPNAKDLASRDVVARSMALEIRAGRGCGPEGDYVLLKLEHLGEDVIKSRLPGIRELS
                     MTFANADPIKDPIPVVPTCHYMMGGIPTNKYAQVISLDDRGRDYIVEGLYAVGECACV
                     SVHGSNRLGGNSLLDLVVFGRAAGLHIHELINAGIEEILISPEEVDFSMQRLHRWNGS
                     QEGERVSDIRQELKKTMQNDFSVFRT"
BASE COUNT          672 a          422 c          551 g          772 t
ORIGIN      
        1 taacttcagg gccaagtgtt ccttcgataa cgggtaggtc aatggaatct gatgcaggta
       61 gcaaaagttc tgcctttttg ccgatcattt gttctccaat acttatatga ttaacttcca
      121 taaatctcag cagttagtgc agtatatatt ttttactgca gggaagaaac ttagtaagca
      181 cacagtctat agttatcctg tgtagacttt attgcaccga taaatgtcgg tcgactataa
      241 tggcctgaac ggaattccat gtaaaaggaa tcattaagcg tgattaccaa ccggcctctc
      301 catattaatc ctttcatggt acgcatgcca atcccagcct tagtttcgat ttcgcatcgc
      361 ctatcgggag tggcaatatt tttactcatt ccattattat tatggattct ggatcaatct
      421 ttaatttcac cagaaaacta tgaaacatta aatacctatt tgcaaaattg gtggctaaaa
      481 cctattgtat ttatttttat cttaagttta ttttttcatc ttatcgctgg aattcgccat
      541 cttttgatgg atatgcatat cggtgaatcg ttacaaggtg gccgtattag ttcgtggata
      601 gtgtttatac ttgcgttttt ggcaagtctt ttttctgcca catggttgtg gggttaaata
      661 tgtcttcacg taaaggattt cgagattggc ttattcagcg gattaccgcc gtttttttag
      721 gtgtttatag cgttatattt ttattatttt ttttgcgtaa tccacaccca acctatatag
      781 actggagcaa ttttttttat caaccgataa tgcaaatatg tacttttatt atgcttatca
      841 gtttgatgtg gcacgcgtgg atcgggcttt ggactattat taccgattac atcaaaccca
      901 tttctttacg actgataact ttaggtttcg taattttaat tcttttttat tattttgcct
      961 ggatattcaa aattctgtgg ggttaatgca tggcaaatcc ggccgttcga tcgtttgatg
     1021 cagtgattgt gggagctggc ggagcaggga tgagggcatc tcttgagtta gcacgagcag
     1081 gaagaaaagt gggattagta tctaaagtgt ttccaacacg ctcgcatacg gtttctgcac
     1141 aaggtggcat aaccgtctcg ttgggaaatc aacatgaaga tcattggcaa tggcacatgt
     1201 ttgataccgt aaaaggttct gattatttag gtgatcaaga ttgtattgag tatatgtgcc
     1261 aagttggtcc cgaggttatt tacgaattgg aacatatggg tcttcctttt tctagaatgg
     1321 aagatggcag aatttaccaa agaccttttg gtggtcaatc gaaaaactac ggcggagaac
     1381 aagcaaccag aactgcagcc gctgcggata gaacaggaca cgctttactg catactttat
     1441 accaaaataa cctaaaagaa gatgttacga tctttagcga atggtatgca ttagatctgg
     1501 taaaaagcca aacaggtcgc attgcaggta ttttggtgct ttccattgaa accggagaag
     1561 tactattgct gaaaggcaaa gctataatat tagctacagg aggcgcaggc caaatttttc
     1621 attcgacgac aaatgctttg atcaataccg gagatggcat tggcatggta ttacgcgcag
     1681 gtttttgtgt gcaagacatg gaaatgtggc aatttcatcc cacggggatt gcaggagtcg
     1741 gtgttttagt cacagaagga tgtcgtggcg agggcggtta tctagtaaac aaagataatg
     1801 agcgctttat ggagcgctat gcacctaatg caaaagattt agcttcccgc gatgtagttg
     1861 cgcgctccat ggctttagaa atacgcgcag gtcgtggctg cggaccagaa ggcgattatg
     1921 ttttactgaa attggaacat ttaggcgaag atgtcattaa atctcgactt cctggcatac
     1981 gtgaattaag tatgacgttt gctaatgcgg atcctatcaa ggatcctata cctgttgtgc
     2041 caacctgcca ttatatgatg ggggggattc ctaccaataa atatgcgcaa gttatttcgc
     2101 tcgacgatcg agggcgtgat tacattgtag aagggctata tgctgtgggg gaatgcgctt
     2161 gtgtttccgt acacggttct aatcgtttag gtgggaattc tttattagat ttagtcgttt
     2221 ttggtagagc tgccggacta catatccatg aattaattaa tgcgggtatt gaagaaatct
     2281 taattagtcc agaagaagtt gattttagta tgcaaagatt acaccgctgg aatggaagtc
     2341 aggaaggtga acgggtaagt gatataagac aagaacttaa aaaaactatg caaaatgatt
     2401 ttagtgtatt tagaacg
//
LOCUS       DASTIB010000111         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_390343, whole genome shotgun sequence.
ACCESSION   DASTIB010000111 DASTIB010000000
VERSION     DASTIB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390343"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFP93_01915"
     CDS             <1..311
                     /locus_tag="VFP93_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843399.1"
                     /translation="YQWDTPSLWNHIAQELPARQPQQLDATQPWLLWRKEIQSMFQSL
                     NPIQFCLYNAILQQATLSELAENLSEMLEPHEIPQVLLQNLQHWITQNVFSKIILAPD
                     "
     gene            315..995
                     /locus_tag="VFP93_01920"
     CDS             315..995
                     /locus_tag="VFP93_01920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET9843400.1"
                     /translation="MQTLAILVADYNLPQLKEQEDTLSYLFETFFKHSGFALKFFQVH
                     KNQWPDFAQYSAYVITGSKESVLKPTPWIDKLEHFIQQGHYEKLIGICFGHQLITKAL
                     GGSVEKGEWHIGVHPIHYTKSIVKHGFQSPLSVRFNHQDHVKKLPPKAELLYTSAKSE
                     FASFKIDDKILSMQFHPEYTQEYHVLLTKKNAHLFTDAVLDETMQNINKPTNHDLILN
                     QIMEFLLI"
     gene            complement(992..1711)
                     /locus_tag="VFP93_01925"
     CDS             complement(992..1711)
                     /locus_tag="VFP93_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843401.1"
                     /translation="MPKDKNEENEKPPVNFEEAQRRIQAALEKNNKLKAQITKYKQPY
                     KQDIDVNPKEQNQSAPLGPDPLHAERFMNDVKAQKGVAPAKKKANNAPELPPFRDAVS
                     NKEAQFTALLNKLKEQNLRQELNQVRKEINNKPTFLFKKMREKLPLGKLTNRNKWIKQ
                     LNQMAEYFLSGDAKNLDLNKKASQVYNDLGEIKDSIGREMHLSNSGMKKLCEDLQNEL
                     KNIQGIEIQVKQSNTQKKTNP"
     gene            complement(2000..2545)
                     /locus_tag="VFP93_01930"
     CDS             complement(2000..2545)
                     /locus_tag="VFP93_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843402.1"
                     /translation="MAKTQHLFQLNKERNKPLPPTPLAPLLTKLAKPSAIKKLYFSKR
                     KEKNLFIHWLKKVSFFNPLGAGKKRSAQIKDLQLTINSLTANINYNDIERATLLYSKL
                     NDIRKEIKFSEIYLFDSALDHVCADLQEEIRNTKIFIPVMEGKNLEDLRFESESKKVA
                     QAIKKREPDTKFLGFNNKFIK"
BASE COUNT          774 a          456 c          465 g          900 t
ORIGIN      
        1 aatatcaatg ggatactcca agtctttgga atcatattgc acaagagctc ccagcccggc
       61 aaccacaaca actcgatgca acccagcctt ggttgttatg gcgcaaagag atccaaagta
      121 tgtttcaatc gttaaacccg attcaatttt gcttatacaa cgctatttta cagcaagcga
      181 ctttaagtga actagcagaa aatttaagtg aaatgctgga accgcatgaa atcccgcaag
      241 ttttattaca aaatctgcag cactggatta cacaaaatgt tttttcgaaa attattttag
      301 ctccagatta atttatgcaa acacttgcta ttttagtcgc ggactataat ttaccccaac
      361 taaaagaaca ggaagacacc ctctcctatc ttttcgaaac tttttttaag catagtggtt
      421 ttgcgctaaa attttttcag gtacataaaa atcagtggcc ggactttgcg caatattctg
      481 cttatgtaat taccggctcc aaagaaagtg tattaaaacc aactccttgg attgataaac
      541 ttgaacattt tattcaacaa ggacactatg aaaaactcat aggaatttgc tttggacatc
      601 aattaattac caaagcgttg ggtggaagcg tggaaaaagg tgaatggcat attggcgtgc
      661 accccataca ttataccaaa agtattgtaa agcatggatt tcaaagccca ctttccgtac
      721 gctttaatca ccaagatcat gtaaaaaaat taccccccaa agccgaatta ctttatacca
      781 gtgccaaatc ggaatttgcg tcgtttaaaa tagatgacaa aattcttagt atgcaatttc
      841 accctgaata tacgcaggaa taccatgtat tgctaaccaa gaaaaatgca catttattta
      901 cggatgcagt gcttgacgaa accatgcaaa atataaataa acccacgaac catgatttga
      961 ttttgaatca aatcatggag tttttattaa tttaaggatt agtcttcttt tgcgtgttag
     1021 actgcttaac ttgtatttcg atgccttgta tattttttaa ttcattttga agatcttcac
     1081 ataatttttt catacctgaa tttgacaaat gcatttccct cccaatggaa tcttttatct
     1141 caccaagatc attataaact tggcttgctt ttttattgag atctaaattc ttagcgtcgc
     1201 cagacaaaaa atattctgcc atttgattaa gctgctttat ccatttgttt ctattcgtaa
     1261 gtttacctaa tggcagcttc tctctcattt ttttaaataa aaaagtgggt ttgttattga
     1321 tttcctttct cacttgatta agctcttgtc ttaaattttg ttctttaagt ttgttaagca
     1381 aagctgtaaa ttgtgcttct ttgttgctta cagcgtctct aaaaggcgga agttcgggtg
     1441 cattattagc tttcttcttc gcaggagcga cgcctttttg tgctttaaca tcattcataa
     1501 atctttccgc atgaagtgga tctgggccca acggggcgga ctggttttgc tcttttggat
     1561 taacatctat gtcttgcttg tagggttgct tgtacttagt gatttgagct ttcaatttat
     1621 tgttcttttc aagggcggct tgtattcttc gctgagcttc ctcaaaattt actgggggtt
     1681 tttcattttc ttcatttttg tctttgggca taaataaacc ttctaaaata tattaatata
     1741 cagaagacaa aaaaaaaata aaaaagttcc taaaagattg ttgtagttac tatatccaca
     1801 aatcgtattt ttgttctaaa tacgactgga taacgcggac aagccgcgtt acgtagggtg
     1861 cccagcactg ttttgaagta gatacgcggg caaacacgtt atatttgaga taagctcgcg
     1921 tttttcagaa gatggataac gcgaacaagt cgcgttatga aggatgcaaa aacaaacgcg
     1981 ttaggaaggc atgaccgtgt tatttgataa atttgttgtt aaatcccaaa aattttgtgt
     2041 cgggttcacg tttttttatt gcttgtgcaa cttttttgct ttcactttca aaacgtaaat
     2101 cctctaagtt cttaccttcc atgaccggga tgaatatttt tgtgttgcga atttcttctt
     2161 gtaaatctgc gcaaacgtga tcaagcgcag aatcaaaaag ataaatttcc gaaaacttta
     2221 tttcttttcg aatatcgttg agtttagaat acaatagggt tgctctttca atatcgttat
     2281 aattaatatt ggcggtaaga ctatttatag taagttgcag atcttttatt tgggctgagc
     2341 gttttttgcc tgcgcctaag ggattgaaga aactgacctt ttttaaccaa tgtataaata
     2401 agtttttctc ttttcttttt gaaaaataga gtttttttat agcagaaggt tttgctaatt
     2461 tagttaaaag tggcgctaaa ggcgtggggg gtagaggttt atttctctct ttatttaatt
     2521 gaaaaaggtg ttgagttttt gccataaaag aaaaggtttt ttgcttgggt aatgtacgaa
     2581 gagactaaca atact
//
LOCUS       DASTIB010000112         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_672624, whole genome shotgun sequence.
ACCESSION   DASTIB010000112 DASTIB010000000
VERSION     DASTIB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672624"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFP93_01935"
     CDS             <1..287
                     /locus_tag="VFP93_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843403.1"
                     /translation="AEPSIRFDATSFLALLQKFRKYGYLEDAEKILKVLIQQNKTGKM
                     DEILAREQLLLARCFYNKSDILKAKHTLEWCKEVYPQTSSAKEAKKLAMY"
     gene            complement(284..1543)
                     /locus_tag="VFP93_01940"
     CDS             complement(284..1543)
                     /locus_tag="VFP93_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843404.1"
                     /translation="MHKKPTPFWKETHRFFLYPFQISMVLIWGRLSIIPLWLAQIEER
                     IPFWILLSTAIFIYSLFLFAILDNALSGKFTPPKLSQKFTGNGIWLILQQPLFISLLI
                     IIYYFSEENLGAEFAYMVTAVVTFCLPAMLTIFSATRQLLHAINPIKITEYILKTHST
                     YFILLGIIYLLEAGTILSYELLNLQLPSVMILHFTAFLTVYISIVIFQLIGYAAYLNH
                     EKLELSVINIEPVEAKKNHPHLLQSQQLIRSGALLEAKKVLHEALQREPLNCDLNDQY
                     HKLMLLMEDKEELQSHGNRYLMFLIRKDYKTKAMQIYLNIYHMNPDYKMNDMELVIKM
                     AILLDQSKKKLPLIHLLRNLHIIYPKHPLVPKAYGFLAKLLFEEFGQEKEAKDILEYV
                     LYNFPNHPIHTAIKKQLNIIENFKQKT"
     gene            complement(1613..2506)
                     /locus_tag="VFP93_01945"
     CDS             complement(1613..2506)
                     /locus_tag="VFP93_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding family
                     protein"
                     /protein_id="HET9843405.1"
                     /translation="MLSQFPLQQKVIETYNDLASKLDELDIAMQHDHELMCWTHGANL
                     SVVKNAQESHRQYAYRILKQVEYLDTQKPREILVCAGFVGASMTTLKIVGELNLAKVQ
                     FKRAMLALRKEKISIRDPYLVENFESMLKMRDYNTANTLQKLGLARLHLKQCYRSIPV
                     LPSAPHKISWTWAHTRSIKKISLQQAEKLLRKKSRDPGIENQLQLLSYLKNHEELAIV
                     QELAPHLRVNIVLNHHTQERIMVKGPIPLFFPADNGTEWPYFKPPKEKSVKNTNRMVR
                     NDVRLEPHAYLPAIRAHRYQQ"
BASE COUNT          821 a          415 c          488 g          874 t
ORIGIN      
        1 gcgcagagcc ttcgattcga ttcgatgcga cctcttttct tgctttatta caaaaattta
       61 gaaaatatgg ttatttagaa gatgcggaaa aaatattaaa agtattaatc caacaaaata
      121 aaaccgggaa aatggatgaa atattagcgc gtgagcaatt gttgctcgca cgctgctttt
      181 ataacaagag tgatattttg aaggcaaaac acacgcttga atggtgcaaa gaggtatacc
      241 cgcaaacaag ttctgctaaa gaagccaaaa aattggctat gtattaggtt ttttgtttaa
      301 agttttctat aatattcaac tgttttttga ttgcagtgtg tataggatga ttggggaaat
      361 tatacaaaac atactctaaa atatctttgg cttccttttc ttgaccaaat tcttcaaata
      421 acaattttgc taaaaacccg taggctttag ggacaagagg gtgtttagga taaatgatat
      481 gcaaattgcg caataaatga attaaaggta actttttttt actttgatct aaaaggatag
      541 ccattttaat tacgagttcc atatcattca ttttataatc cggattcata tgataaatat
      601 taagataaat ttgcattgcc ttggttttat aatctttgcg tattaaaaac atcaaatagc
      661 ggttgccatg gctttgtaat tcctctttat cttccatcaa taacattagt ttatggtatt
      721 gatcgtttaa gtcacaattg agaggttcac gttgtaaggc ttcgtgtaaa accttttttg
      781 cctcaagtaa agcccctgat cggatgagct gttgtgattg gagcaaatgg ggatggtttt
      841 ttttggcttc tacaggttca atattaataa ccgaaagctc taatttttca tggttgagat
      901 acgctgcata tccgatgagt tgaaatatta caatactgat atataccgta agaaatgcag
      961 taaaatgcaa gatcattacg gagggtaatt gtaagtttaa aagttcatac gataaaatag
     1021 tgccagcctc gagtaaatag atgatcccta ataaaataaa atacgtacta tgtgttttta
     1081 atatatattc tgttatctta attgggttga tagcgtgaag cagctgtctc gttgcactga
     1141 aaatagtaag catagccggt aaacaaaaag ttaccacagc agttaccata taagcgaatt
     1201 ctgcgcccag attttcttca ctaaaataat aaataataat tagcaaacta ataaataggg
     1261 gttgctgcag tattaaccaa atcccgttac ctgtaaattt ttgcgaaagt ttcggtggcg
     1321 taaatttacc ggagagcgca ttatccaaaa tggcaaacaa aaaaagacta tatataaata
     1381 tagcggtgct taataaaatc caaaaaggga ttcgttcttc tatttgtgca agccacagcg
     1441 gaataatgct taatcttccc caaatcaata ccatggatat ttgaaaaggg tatagaaaga
     1501 aacgatgtgt ttctttccaa aagggtgttg gttttttgtg catacaaatt tttggtttcc
     1561 taaatttaag tttaggaatt tataagcggt atttgggtga tcgtaaaaat agctactgtt
     1621 gatagcgatg cgcacgtata gcaggtaaat aagcgtgcgg ttctaagcgt acatcgttac
     1681 gtaccattct atttgtattt ttaacgcttt tttctttggg tggtttaaaa tacggccatt
     1741 cggttccgtt atcggctgga aaaaatagag gaatgggtcc ttttaccata atccgttctt
     1801 gtgtatggtg atttaacaca atattaacac gtaaatgtgg agccaattct tgcacaatag
     1861 ccagctcttc atgatttttt aaataagaca gtagttgtaa ttgattttca attccgggat
     1921 cgcgactttt ttttcttaat agtttttcag cttgttgtaa actaattttt ttaatggagc
     1981 gggtgtgtgc ccaagtccaa ctaattttat gtggtgcgct aggtaataca ggaatagaac
     2041 gatagcattg tttcagatgt aaacgagcta accctagttt ttgtaaggta ttcgctgtat
     2101 tgtaatcccg catttttaac atcgattcaa aattttctac taaatacgga tcacgaatag
     2161 aaattttttc tttacgcaaa gcaagcatcg cacgtttaaa ttgaactttc gctaagttta
     2221 attcgcctac tatttttaaa gttgtcatgg atgcgcctac aaaacccgca cacactaata
     2281 tttcacgcgg tttttgcgta tccaaatact ctacttgttt taagatccga taggcatact
     2341 ggcgatgact ttcctgcgcg ttttttacca cagataaatt cgcaccatgt gtccagcaca
     2401 taagctcatg atcatgttgc attgcaatat ctaattcgtc tagtttacta gccaagtcat
     2461 tgtaggtttc aattactttt tgctgtagcg ggaattgaga aagcataaat gttactcggt
     2521 agcgaagggt gtattttaga aaatttcttt ggtttatagc aacatgcgag cagtttaaat
     2581 ttacagaaaa ttaaatgt
//
LOCUS       DASTIB010000113         6737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_346207, whole genome shotgun sequence.
ACCESSION   DASTIB010000113 DASTIB010000000
VERSION     DASTIB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346207"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFP93_01950"
     CDS             complement(<1..273)
                     /locus_tag="VFP93_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET9843406.1"
                     /translation="MRRIKRIHFIGIGGAGMGGIAEVLLTQGFEVSGSDLHESAMTQR
                     LKKIGANVYEGHACEYVRGADVVVVSAAIPQNNPEIQEALAARIPIV"
     gene            complement(311..1399)
                     /gene="murG"
                     /locus_tag="VFP93_01955"
     CDS             complement(311..1399)
                     /gene="murG"
                     /locus_tag="VFP93_01955"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9843407.1"
                     /translation="MSSNKCFVIVAGGTGGHVMPAIAVAETLKESGFSIHWLGTQKGI
                     EARIVPPLGIPLHFIPVQGLRGKGFKSWLLAPFQILRALIEALKILNTLKPCGVLSMG
                     GYVTGPAGLAAWILRIPLILHEQNSISGLTNRLLAPFAKRILLGFPKTFTQSKAHVTG
                     NPIRKSILQALPKTMRNTSDPLRILIIGGSLGAHFFNQIVPKALKLCSRAFAFEVWHQ
                     TGEKEFVETQLRYEEINSVRVEPFIENMGEAYVWADLVICRSGALTISELAAIGVPSI
                     LIPFPHAVDDHQSYNARYLVDANAAILLPQSLLTPECLSKTIKQLAQDRQKLSLMADK
                     CRSVAQIQATEQVAKNCIEVSCGISPSL"
     gene            complement(1389..2543)
                     /gene="ftsW"
                     /locus_tag="VFP93_01960"
     CDS             complement(1389..2543)
                     /gene="ftsW"
                     /locus_tag="VFP93_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9843408.1"
                     /translation="MADKSQAWLKWRFDPTLMTAVSCLLMLGLVMVTSASMVVAEKRM
                     GSAFYYAFHHAAYLSLGLLIAVFASQMGVRFWQKISGILLIVTLIMLALVLMPIIGKK
                     VNGSSRWLNLGLLTLQVSELAKIFIIIYMASYVARHEEAVKTHWIGFLKPLSLLILVG
                     GFLLLEPDFGAMVVIMSTGLGMLFLAGVPFRYFFVLVALMVVSFGGLMVSAPYRLKRL
                     TAFLDPWAYQYDSGYQLTQALIAFGRGEWIGVGLGSSVQKLFYLPEAYTDFVFAVLAE
                     ELGLIGALSVVILFVIFIYRGITIGLLAERRGDRFAGFLVYGLTFWIGLQALINMGVN
                     TGLLPTKGLTLPFLSYGGSSLLAICFALGLIFKIDRDNRIYFRREAGFVV"
     gene            complement(2583..3911)
                     /gene="murD"
                     /locus_tag="VFP93_01965"
     CDS             complement(2583..3911)
                     /gene="murD"
                     /locus_tag="VFP93_01965"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9843409.1"
                     /translation="MAYVVLGLGRTGLAVVRYLKKRQFPIIVADTRMQPPNLKIFQQK
                     FPEVVLHVGALPIEAMLNAQAVIAPPGLALTHPIFETLKEKNVPVWGDIELFAQQAQA
                     PAIAITGANGKSTVTSLVGAMAKSSNLDSLVGGNIGVPALDLLESQTHPQCYVLELSS
                     FQLETTYSLKPQVACILNITRDHLDRHGSLDAYVKAKQRIYHGAQWAIYNRHDVLTKP
                     TFDVPHSITFGLDEPKTGHFGIRTHNGLRWVAYGSELWLPVNKILLFGRHNVLNALSA
                     LSIGTAFGFSKESMLKELQTFKDLKHRCQFIKKLHEVTWINDSKGTNVGATVAAIESV
                     GEHIHGKIVLIAGGKAKDTDFSDLKPWVSKYVKQVLLIGQDGHLVGDALKDCAPCLEA
                     GTMNAAVSLASNLAKPGDAVLLSPACTSLDVYANFEERGDAFVQAVQEVK"
     gene            complement(3928..5010)
                     /gene="mraY"
                     /locus_tag="VFP93_01970"
     CDS             complement(3928..5010)
                     /gene="mraY"
                     /locus_tag="VFP93_01970"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9843410.1"
                     /translation="MLLALADVLTRFNHAFQVFHYITLRSILAALTALAFSLCFGPTL
                     IRWLKVKQLGQMVRDDGPQSHFSKKGTPTMGGALILLAITVATLLWADLRNRYVWITL
                     FVTLSFGLIGWVDDYRKIIKRNSKGLPARWKYLYQSFVGFAAVIYLYFQATTPQETAL
                     IFPFFKDWILPLGIFYIFFCYFVIVGSSNAVNLTDGLDGLAILPTVLVGGALGVFAYV
                     AGHKEFAEYLAVPYIYGAGELAIFCSSIVGAGLGFLWFNSYPAQIFMGDVGALALGAA
                     LGIVAVIVRQEIVLFIMGGIFVLETLSVILQVASFKLTGQRIFKMAPIHHHFELIGWP
                     EPKVIVRFWILTVILVLLGLATLKLR"
     gene            complement(4991..6361)
                     /gene="murF"
                     /locus_tag="VFP93_01975"
     CDS             complement(4991..6361)
                     /gene="murF"
                     /locus_tag="VFP93_01975"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET9843411.1"
                     /translation="MMRLSQVKKILNAELIGQDVSFDAVSTDSRTLPRDSLFVALTGE
                     NFDGHNFIEDALKKGAKAILVAQKEVVPQSASALLVADPIEALAKLARFHRAQFSIPC
                     IGVTGSCGKTTVKSMIGAILSRVGKTLISQGNFNNHIGLPLTLFNLKKEHQFIVVEMG
                     ASAPLEIAYLGQVAQPTIALITNVHNAHLSGFGSLENVAKTKAEIYEALDTDGVAILN
                     HDSPYAAFWQKIIGFRKTVTFGIHKPAAIKADKIHYNHFKTAFRVATPKEQFEVTLNL
                     PGEHNVQNALAAIACIYHLNIPKALIQEALYAYQGVPGRLTPHELSNGIQLIDDTYNA
                     IPDAVEAAIEVLAKSEHEKLLVMGDMAELGVHSEMFHERMGAKAKKVGIDHLWAVGPL
                     SQSMVKAFGVQGRWFASKAELMAELKKIDKPMTILIKGSRSSGMEELVDGLLLSRKAS
                     YAASAR"
     gene            complement(6358..>6737)
                     /locus_tag="VFP93_01980"
     CDS             complement(6358..>6737)
                     /locus_tag="VFP93_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9843412.1"
                     /translation="RSICKGKLWCVFGCGGDRDLLKRKVMGKIATDFADQIIITNDNP
                     RTEEPKAIIRDILQDLTPKELAKTLVEEDRANAIAYAIQQALKDDVVLIAGKGHEDFQ
                     IIGNKKIPFSDIQTVKKIVGESL"
BASE COUNT         2120 a         1500 c         1203 g         1914 t
ORIGIN      
        1 aacaatagga atgcgtgcgg ctaaggcttc ttgaatttcc ggattatttt gtggaattgc
       61 tgctgaaaca accacaacgt ccgcgcctct aacatattcg caggcatggc cttcataaac
      121 gttggcgcca atttttttta ggcgttgagt catagcactt tcgtgtagat ccgaaccaga
      181 gacttcaaag ccttgggtta ataagacttc cgcgatacca cccattcccg caccacctat
      241 tccaatgaaa tgaatacgtt ttatgcggcg catagacaat gggtcggact tacgattata
      301 aatacgttga ttaaagggag ggtgaaattc cacaactaac ctcaatacaa ttcttagcta
      361 cttgttctgt agcctgaatt tgcgctactg aacgacactt gtcagccatt aaacttaatt
      421 tttggcgatc ttgagccaat tgtttaatgg ttttacttaa gcattctggc gtaagcaaag
      481 attgaggcag taaaatagct gcgtttgcat ccaccaagta acgagcatta taactttgat
      541 ggtcgtcaac tgcatgtgga aatgggatca aaatactagg aacacctatg gcagctaact
      601 cagaaatggt gagtgctcct gatctgcaaa taacaagatc cgcccatacg taggcttcac
      661 ccatattttc tataaagggt tcaacacgca cagagttaat ttcttcataa cgcaattggg
      721 tttctacgaa ttctttttct ccggtttgat gccacacttc gaaagcaaac gcacgcgaac
      781 atagctttaa agcctttggc acaatttggt tgaaaaagtg cgcgcctaaa ctacccccta
      841 taattaaaat ccgcagcgga tccgaagtat tacgcatcgt tttgggcagc gcttgcaata
      901 tactttttcg aatcggattg ccagttacat gtgctttgga ttgggtgaat gttttaggaa
      961 accccagtaa aattcttttt gcgaatggag cgagtaaacg attcgttaaa cccgaaatag
     1021 aattttgttc atgcaatatt aaaggaatgc gtaaaatcca agccgctaat cccgcggggc
     1081 ctgtaacgta accacccatg cttaacacac cacaaggttt cagagtgttt aaaattttta
     1141 atgcctctat taaggcgcgt aatatttgaa atggtgctaa cagccaactt ttgaacccct
     1201 tcccccgtaa cccttgtacc ggaataaaat gtaatggaat acctaaaggt gggactatac
     1261 gcgcttctat acctttttgc gtacctaacc aatgaataga aaagccagat tcttttaagg
     1321 tttcagccac agctattgcc ggcataacat ggccgcctgt gcctcctgca actatgacaa
     1381 aacatttgtt agacgacaaa accggcctct cttctaaaat aaattcgatt atctcgatca
     1441 attttaaaaa taagtcctaa tgcgaaacat atcgcgagca agctactacc accataactt
     1501 aaaaaaggaa gcgttaatcc tttcgtaggt aataatcccg tatttactcc catattaatg
     1561 agcgcttgta aacctatcca aaaggttaaa ccgtacacca gaaagcctgc gaatcgatcg
     1621 ccgcggcgtt ctgcaagcaa gccaatggtt atgcctcgat aaataaaaat gacaaaaagt
     1681 atgaccaccg atagtgcacc tatcagccca agttcttctg ctaacacggc aaatacaaaa
     1741 tcggtatacg cttctggtaa ataaaataat ttttgcacgc tgctacctaa acctacccct
     1801 atccactcgc ctcgaccaaa cgcaataagt gcttgggtta attgataacc agaatcgtat
     1861 tgatacgccc aaggatctaa aaaagcagtt aaacgtttta aacgataagg tgcgcttacc
     1921 attaagccac cgaaacttac taccatgaga gcgactaata caaaaaaata acgaaaaggt
     1981 accccagcga gaaacaacat gcctaatcct gtggacatga ttacaaccat ggcaccgaag
     2041 tcgggttcga gtaacaaaaa accaccgaca agaataagta agctcaaagg ttttaaaaaa
     2101 cctatccaat gcgtttttac tgcttcttca tgacgagcta cataacttgc catataaatt
     2161 atgatgaata ttttcgctaa ttccgaaact tgaagggtca gcaaacctaa atttaaccag
     2221 cgcgatgaac catttacttt tttgccaata attggcatta agactaaagc caacataata
     2281 agtgttacaa taagcaaaat gcctgaaatc ttttgccaaa aacgcacacc catttggctt
     2341 gcgaaaacag caatcaataa gcctaagcta agatatgctg catggtgaaa agcataataa
     2401 aaagcagaac ccatgcgttt ctctgctact accatggaag cagatgtcac cataactaaa
     2461 ccaagcatta acaaacaaga aactgctgtc atcaaggtgg gatcaaaccg ccactttagc
     2521 caagcttgtg acttatctgc catatttatc ctttattgtg gaaccatgtg aaataatttg
     2581 gattatttta cttcttgaac ggcttgcaca aaagcatcgc cccgttcctc aaaattggca
     2641 taaacgtcta aactagtaca cgctggcgat aaaagtacag catctccagg ctttgcgaga
     2701 ttagatgcta aggatactgc agcattcata gtgcctgctt caagacaagg tgcacaatct
     2761 ttaagtgcat cccctactaa gtgaccatct tggcctatta ataacacttg tttaacgtac
     2821 ttagacaccc atggttttaa atcgctaaaa tctgtatctt ttgctttacc accagcaatt
     2881 aaaacaattt ttccatggat atgttcgccc acagattcaa tagctgctac tgttgctcct
     2941 acattggttc ctttcgaatc gtttatccaa gtaacctcat gcaatttttt tatgaactgg
     3001 caacggtgct ttaaatcttt aaaagtttgc aattctttta acatcgattc tttcgaaaat
     3061 ccgaaggcgg taccaatact taaggcactt aacgcattta aaacattgtg tcgtccaaat
     3121 aataaaattt tattgactgg caaccacaat tcagaaccat aagcgaccca acgtaaccca
     3181 ttgtgggttc gaattccaaa atggcctgtt tttggttcat ccaaaccgaa agtaatagag
     3241 tgtggcacgt caaaagtagg ttttgtcaat acatcgtgac gattgtaaat agcccattgt
     3301 gcaccatggt aaatacgttg ctttgctttt acatacgcat ccaaggaacc atgtcgatca
     3361 agatgatcac gcgtaatatt taaaatacag gcaacctgtg gttttaagga ataggtggtt
     3421 tcgagttgga agctagataa ttcaagaaca taacactgcg ggtgtgtttg cgattctaat
     3481 aagtcaagtg ccggaactcc gatgttacct ccaactaaag aatcgagatt tgaagatttt
     3541 gccattgctc caactaatga agttactgtg ctttttccgt ttgctccggt tatggcaata
     3601 gcaggagctt gtgcttgttg agcaaaaagt tctatgtctc cccaaacagg tacatttttt
     3661 tctttgaggg tttcaaaaat aggatgcgtt aaagctagtc ctggtggtgc tatcaccgct
     3721 tgcgcgttaa gcatagcttc gatcggcaag gcacccacat gcaaaacaac ttccggaaat
     3781 ttttgttgaa aaatttttaa attgggcggt tgcatgcgtg tgtccgctac aataattgga
     3841 aattgtcttt tttttaaata acgtactacc gctaaaccag tacgtcctaa gcccaacaca
     3901 acatatgcca tatactaacc ttttccatta acgcaattta agcgttgcaa gacccaataa
     3961 cactaaaata actgttaaaa tccaaaagcg tacaataact tttggttcag gccaccctat
     4021 taactcaaaa tgatgatgaa tgggagccat tttaaaaata cgttgacccg tcaatttaaa
     4081 agaggcaact tgcaaaatca cggaaagggt ttccagcaca aaaattccac ccataataaa
     4141 taaaacaatt tcctggcgca caatcacggc aactatacct aaagctgctc caagtgctaa
     4201 agccccaaca tcacccatga atatttgtgc cgggtaggaa ttaaaccata aaaatcccaa
     4261 tcctgctcca acgatactcg aacaaaaaat agcaagttca cccgcaccat aaatgtaagg
     4321 cacggctaaa tattcggcaa attctttatg tcctgcaaca taagcaaaca cccctaacgc
     4381 acctcctacc aaaaccgtag gcaaaattgc cagcccatcc aagccatcgg ttaaattaac
     4441 ggcattggaa gaacctacaa tgacaaaata acaaaaaaat atataaaata tgcccagggg
     4501 taaaatccaa tctttaaaaa aaggaaaaat taatgcggtt tcttgaggtg ttgtggcttg
     4561 aaaatataaa taaattaccg cagcaaatcc aacaaaggat tgatacaaat acttccatcg
     4621 tgcaggcaat cctttggaat tgcgttttat aatttttcga taatcatcga cccaacctat
     4681 taagccaaaa cttaacgtaa cgaataaagt aatccacaca taacgattac gcagatccgc
     4741 ccacaaaagt gttgcaacgg taatcgctaa taaaattaga gcgccaccca tcgtgggtgt
     4801 tccctttttg ctaaaatgcg attgaggccc gtcgtctctc accatctggc ctaattgttt
     4861 tacttttaac caacgaataa gcgtaggccc aaaacataag gaaaacgcta gcgctgtaag
     4921 cgctgccaag atcgaacgta atgtaatgta atgaaatacc tggaaagcat gattaaaacg
     4981 cgttaataca tcagcgagcg ctagcagcat acgatgcttt ccttgaaaga agtaaaccat
     5041 caacaagttc ttccatacca gaagatcttg aaccttttat taaaatagtc atgggcttat
     5101 ctattttttt taactccgcc attaattctg ctttacttgc aaaccaacgt ccttgcacac
     5161 caaaagcttt gaccatggat tgactcaaag ggccgaccgc ccacaagtga tctatgccca
     5221 cttttttcgc ttttgcaccc atgcgttcat ggaacatttc tgaatgcaca cctaattctg
     5281 ccatgtcgcc catgactagg agcttttcgt gttcactttt cgctagtact tcaatcgccg
     5341 cttctactgc atctggaatc gcattgtagg tatcgtcaat aagctgaata ccattgctta
     5401 gctcatgggg agttaatcga ccaggaaccc cttgatacgc gtatagagct tcttgaatta
     5461 atgcttttgg gatatttaaa tgataaatac aagcgatagc tgctaaagca ttttgtacat
     5521 tatgttcacc aggaagattg agcgtaactt caaactgttc tttgggtgtg gcaactcgaa
     5581 atgctgtttt aaaatggtta tagtgtattt tatccgcttt aatggcggca ggcttgtgaa
     5641 tcccaaaggt aaccgttttg cgaaatccta taattttttg ccaaaatgca gcataaggac
     5701 tatcgtgatt caaaatagct acgccatcgg tatctaacgc ctcataaatt tctgccttag
     5761 ttttagcaac attttctaaa gaaccaaacc ctgacaaatg tgcgttatgc acattggtga
     5821 taagggctat tgtaggttgc gccacttgac ctaagtaggc aatctctaaa ggtgcactcg
     5881 cccccatctc taccacaata aattgatgtt ctttttttaa gttgaataac gttaagggta
     5941 acccaatatg attattaaaa ttgccttgtg aaattagcgt tttacctacc cgagataaaa
     6001 ttgcaccaat catactttta actgtggttt taccacagct tccggtcacg ccaatacaag
     6061 gaatagaaaa ttgtgcccga tgaaaacgtg caagcttagc caaagcctct ataggatcgg
     6121 caactaataa agcagacgcg gactgaggca ctacttcttt ttgtgccaca agaattgctt
     6181 ttgcaccttt ttttaaagcg tcctctataa aattatgccc atcaaaattt tcacccgtta
     6241 atgctacgaa caaactatct cgtgggagcg ttctgctatc ggtacttact gcatcaaatg
     6301 atacatcttg tccaattaat tcagcattta aaattttctt aacttgggat agtcgcatca
     6361 taaagattcg cccactattt tttttacagt ttgaatatca ctaaaaggga tttttttatt
     6421 tccaatgatt tggaaatctt catgcccttt acccgcaatt aaaaccacat catcctttag
     6481 cgcttgttga attgcgtatg caattgcatt tgccctatct tcttcaacaa gtgtttttgc
     6541 caattccttt ggcgtcaaat cttgcaaaat atcgcgaata atcgctttag gttcttcggt
     6601 tctaggatta tcatttgtga tgataatctg atcggcaaaa tccgttgcaa ttttgcccat
     6661 tactttgcgt tttagtagat cgcgatcccc tccgcaaccg aatacacacc ataattttcc
     6721 tttacaaata gagcgcg
//
LOCUS       DASTIB010000114         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_508973, whole genome shotgun sequence.
ACCESSION   DASTIB010000114 DASTIB010000000
VERSION     DASTIB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508973"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..553
                     /locus_tag="VFP93_01985"
     CDS             23..553
                     /locus_tag="VFP93_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9843413.1"
                     /translation="MGIRTFQNCSPKFGNAVFVDTRATVIGDVDIGDNASIWPNTVLR
                     GDLLRITIGKNTNIQDGSVGHTTHASGFNLKGNALFVGDNVTVGHHALLHGCTIQNNV
                     LIGMQSIILDGAIIESNIILGAGSLVSPGKHLQGGYLYFGRPAKMQRLLTEEEVKMIQ
                     YSAESYVKLKKTYLGK"
     gene            complement(567..1388)
                     /gene="aroE"
                     /locus_tag="VFP93_01990"
     CDS             complement(567..1388)
                     /gene="aroE"
                     /locus_tag="VFP93_01990"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET9843414.1"
                     /translation="MKLTLFGSSIQHSLSPKIYEEFARQFQIDLCYTKTVVTCSQDLK
                     IALDTFRDSGGVGANITSPLKQEAYMLCDSVSQHALDIKAINVLVWKDHWYGTNTDGA
                     GFIADLSRLKIDLKDLNILILGAGGAVRALLPLLLTKQPQNIFIANRTYTNILAFASD
                     LTKVTPLALHALVKMDLKFDLIINAIPNFPENTFEALSKFIPKSCVYDLSYKENHPTP
                     FLQWAAHLGAYLCADGIGMLVEQAALSFNEWFGVMPPITVKLELMKKYSFKKNQL"
     gene            complement(1538..2146)
                     /locus_tag="VFP93_01995"
     CDS             complement(1538..2146)
                     /locus_tag="VFP93_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843415.1"
                     /translation="MQEGPRVKKNREGIKETHFATEKRLPGKGNIPDMHVKINKRHNT
                     ATRHAHKNTRKIVQPLFSLEKVMVAVCATVVIGTIGWYFVALTLGALAGIAVGIGVLA
                     TGTSNQSSTQTPKKRSARKPEEIPVEDPNKPSISQLIPNYQEQKNIDEEMLTKINQLN
                     EKLGKVILEGQQNFDNNLLEKQHNFDKELLEQEEKLRHAFRF"
BASE COUNT          717 a          431 c          466 g          797 t
ORIGIN      
        1 aattttatta aggtaaaaaa atatgggaat tcgaactttt caaaattgtt ctcctaaatt
       61 tggtaatgct gtttttgtgg atacacgtgc aaccgttatc ggcgatgtag acattgggga
      121 taacgcttct atttggccga atactgtgct cagaggtgat ttgttacgca taaccatagg
      181 aaaaaatacg aatattcaag atggttctgt tgggcacacc acgcatgcta gtggtttcaa
      241 ccttaaagga aatgcgctat ttgtaggcga taatgtgacg gtgggtcatc atgcactttt
      301 gcatggctgt accattcaaa acaatgtgct tatcggaatg caaagtataa ttctggatgg
      361 tgctatcata gaatccaata ttatccttgg agcgggtagt ctagtttctc ctggaaagca
      421 tttgcaaggt ggttatttat atttcggaag accggcaaaa atgcaacgcc tgttaaccga
      481 agaggaagtg aaaatgattc aatacagtgc agaaagttac gttaaattaa aaaaaaccta
      541 tttaggcaaa taggtttttg tcattatcat agttgatttt ttttgaaact atattttttc
      601 attaattcaa gtttaactgt gatcggtggc atgacgccaa accattcgtt aaaagaaagc
      661 gccgcttgct caacaagcat accaatacca tctgcgcata agtaagcacc taaatgcgcg
      721 gcccattgca aaaaaggcgt aggatgattt tctttgtaag ataaatcata tacacaactt
      781 ttgggaataa atttagacaa agcttcgaag gtattttctg gaaaatttgg aatcgcatta
      841 ataatcaaat caaatttaag atccattttt actaatgcat gaagtgcaag aggtgtaact
      901 ttggtcaagt ctgaagcaaa tgctaaaata ttggtatagg ttcgattcgc aataaagata
      961 ttttgaggtt gttttgttaa caataaaggc agcaaagctc taactgcacc acctgctccc
     1021 aatataagaa tatttagatc tttcagatct atcttcaatc gagataaatc tgctataaat
     1081 ccagcgccat ccgtattagt tccataccag tgatccttcc acaccaatac attgattgcc
     1141 tttatatcta aagcatgttg tgaaacacta tcgcaaagca tatacgcttc ttgttttaaa
     1201 ggagacgtaa tattggcgcc aactccccct gaatctcgaa aagtgtctaa cgctattttt
     1261 aaatcctggc tgcaggttac tacggttttg gtgtaacaca aatctatctg aaattgtcga
     1321 gcaaattctt catagatctt aggggaaagg ctatgctgaa tagatgaacc aaacaaagta
     1381 agtttcataa acaccttggg agttatgctt ttcgcagatg tttcggtctt gttaacttcg
     1441 ccatttcata ctcaatatgt attacttata tatttccagt tgctaagtgc gtcggcgtct
     1501 gtggaaaaat ccctgctttg tcatacaaaa ctactattta aaatctaaaa gcatgccgaa
     1561 gtttttcttc ttgttcgagg agttctttgt cgaaattatg ctgtttttcg agtaagttgt
     1621 tatcgaaatt ttgctgtcct tcaagaataa cttttcccaa tttttcattt aattgattta
     1681 tttttgttag catttcttca tctatatttt tttgttcttg ataatttggg attaattgag
     1741 aaattgaagg cttattggga tcttcaacag gaatttcttc gggtttgcgc gcacttctct
     1801 ttttaggagt ttgcgttgaa ctttgattag acgttcccgt tgctaaaacg ccgataccca
     1861 cggcaatgcc agctaaagcg cccagggtga gtgcaacaaa ataccaacca atggttccaa
     1921 taactaccgt tgcacaaaca gcaaccatga ctttttctaa actgaataga ggttgaacga
     1981 tttttctcgt gtttttgtgt gcgtgtcttg tcgccgtatt atggcgctta tttattttga
     2041 catgcatgtc aggaatattg ccttttcccg gtagtctttt ttccgtggca aagtgtgttt
     2101 ccttgatacc ttctcgattt tttttgactc ttggtccttc ttgcatacta ctaacctcac
     2161 acaaaaaaaa ttatcataaa caaaaacatc gttttcttct aacaaaagag ggtgattgca
     2221 aaattaatta atctttcaaa ctaaaccaaa gaattggcta agataaaaat atatttttgt
     2281 tgtacggttt ataggaatga cttggctttg aaagtaagag cgggttttcc tgcttgcggg
     2341 taggttttca taagatatgg attcccgcat tcgcgggaac gacggagaag ccgtagttcg
     2401 cgagggaaga c
//
LOCUS       DASTIB010000115         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_21132, whole genome shotgun sequence.
ACCESSION   DASTIB010000115 DASTIB010000000
VERSION     DASTIB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21132"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFP93_02000"
     CDS             <1..256
                     /locus_tag="VFP93_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET9843416.1"
                     /translation="QEEITIYEAKGCDQCNEGYKGRVGIYEVMPVSDAIGRIIMEGGN
                     AIDIRDQMAKDGVINLRRAALNKVKEGLTSLEEVNRVTKD"
     gene            259..1506
                     /locus_tag="VFP93_02005"
     CDS             259..1506
                     /locus_tag="VFP93_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9843417.1"
                     /translation="MPAVKKATPTTTAPKSATFTYEVTNQQGKTIKGELTALNLALAK
                     AELRRQGFTPVKVRRKNKSFLSGVRKKRISSEDISFYSRQMATMMTAGVPLVQSFDIV
                     ARGTDNLSLRDLILSVKAEVEAGRSYAEALEKFPQHFDHLFCNLVAAGEASGSLETML
                     DRIATYKEKAESLKRKIKKALFYPIAVMIVAVVVTFILLIFVVPQFEQLFKGFGADLP
                     AFTRMVINISEFVQSYWYIILGLAIIGIWAFIYFKKRSTKFQHMLERLSLKIPIIGNI
                     LHKASIARYARTLATTFAAGVPLIEALDSVAGATGNIVYAEAVWRIRDDVAMGSQVQA
                     SMRSTNIFPNMVTQMVAIGEESGSLDEMLSKVADIYEEEVDAAVDGLSSLLEPLIMVI
                     LGVLVGGLVIAMYLPIFKMGSVV"
     gene            1507..>2301
                     /locus_tag="VFP93_02010"
     CDS             1507..>2301
                     /locus_tag="VFP93_02010"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET9843418.1"
                     /translation="MEWIQNIQYNIFLLLTLSALFGLMIGSFLNVAIYRLPIMLQSEW
                     ITECTSFLKDYYSRNQPQKDVPEYTPEKSMNLAWPSSFCPHCKTPLKLRENIPIISFL
                     WQRGRCNHCATKIDWRYPLVEIVCAIATTITVWEFGVNWICWLALLLTWSLIVLSFID
                     FEHQILPDNITLPFLWFGLFLNAFLVFTTPNNAIIGTIAGYLSLWCVYQLFKFFTGKE
                     GMGYGDFKLMALLGAWLGWQLLPLIIVLSSFLGVIIGLSLILFKGHD"
BASE COUNT          668 a          401 c          499 g          733 t
ORIGIN      
        1 acaagaagaa attacaatct atgaggccaa aggatgtgac caatgcaacg aaggctataa
       61 gggacgcgtc gggatttatg aggttatgcc cgtgagtgat gcgattggtc gtatcattat
      121 ggaaggaggg aatgccatcg atatacgcga tcaaatggcc aaagatgggg ttattaacct
      181 cagacgtgct gctttgaata aagttaagga gggtttaacc agtttggaag aagtaaatcg
      241 cgttaccaag gattaagtat gccagcagta aaaaaagcaa cgcccaccac cactgctccc
      301 aagagcgcaa ctttcaccta tgaagttacc aatcaacaag gtaaaactat taaaggtgaa
      361 ttgacagctt tgaatttagc gctggcaaaa gcagagttgc gtcgtcaagg ttttacacct
      421 gttaaagtac gtcgtaaaaa taaatctttt ttaagcggag tacgaaaaaa aagaattagt
      481 agtgaagata tttcctttta tagtcgtcaa atggcaacca tgatgaccgc tggtgtaccc
      541 ctggtgcaat cctttgatat tgttgcacgt ggtaccgaca atttatcgtt gcgcgatctt
      601 attctttccg taaaagcaga agttgaggca ggtagatcct atgcagaagc tttagagaag
      661 tttccacaac attttgatca tttgttttgt aacctggtag cagccggtga agcttctggt
      721 tcgttagaga ctatgttaga tagaattgct acttataaag aaaaagcaga atcgttaaaa
      781 agaaaaatta aaaaagcgct attttaccct attgcggtca tgatagtagc cgttgtagta
      841 acgttcatct tattaatatt tgtagtgcca caatttgaac aactctttaa agggtttggc
      901 gctgatttac ctgcttttac tagaatggta attaatattt ctgaatttgt gcaaagttat
      961 tggtatatta tactcggttt ggctattatc ggaatttggg cctttatcta ttttaaaaaa
     1021 cgctccacga aattccaaca catgttagag cgcctgtcgt tgaaaattcc tattattggt
     1081 aatattttac acaaagcatc gatcgcacgt tatgccagaa cgcttgctac tacatttgca
     1141 gccggggtgc cgcttatcga agcattagat tctgtcgctg gagctactgg caatattgtt
     1201 tatgcggaag ccgtgtggcg catacgcgat gatgtggcca tgggttcaca agtccaagct
     1261 tctatgcgat ccaccaatat ttttcccaat atggtaaccc aaatggtggc aattggggag
     1321 gaatctggtt ctttagatga gatgcttagt aaagttgcgg atatttatga agaagaagtc
     1381 gacgcagcag tcgatggatt aagtagtttg cttgaacccc ttatcatggt tatattaggg
     1441 gttttggtgg gtggactggt gatcgcaatg tacttaccca ttttcaaaat gggatccgtg
     1501 gtataaatgg aatggattca aaatattcaa tataatattt tcttattatt aaccctttca
     1561 gctttatttg gtttaatgat aggttcattt ctcaatgtag cgatttatcg tttaccgatt
     1621 atgctacaat ccgaatggat cacagaatgc acctcttttc tcaaagacta ttattcgcgc
     1681 aatcaaccac aaaaagatgt gccggaatac acgccagaaa agagcatgaa tttagcctgg
     1741 ccttcttctt tttgtccgca ttgtaaaacg cctttaaagt tacgcgaaaa tatcccgatt
     1801 ataagttttt tatggcaaag aggtcgctgc aaccattgtg caaccaaaat tgattggcgt
     1861 tatcccttag tagaaatagt atgcgctatt gcaactacca ttacggtttg ggagtttggt
     1921 gtcaattgga tatgttggct tgcattgctg ttaacgtgga gcttaattgt tttaagtttt
     1981 atcgactttg agcatcaaat tctacccgac aatataacat tacctttttt atggtttggt
     2041 ttatttttaa acgcttttct ggtattcaca acgccaaata atgcaattat aggtactatt
     2101 gcgggctatt taagtttgtg gtgtgtgtac caattattta aatttttcac cggaaaagaa
     2161 ggtatgggct atggtgattt taaacttatg gcattattgg gcgcatggtt agggtggcag
     2221 ctcttgccac ttattattgt actttcttcg tttcttggtg tgataatagg acttagccta
     2281 attctattta aaggacacga c
//
LOCUS       DASTIB010000116         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_443960, whole genome shotgun sequence.
ACCESSION   DASTIB010000116 DASTIB010000000
VERSION     DASTIB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443960"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFP93_02015"
     CDS             <1..237
                     /locus_tag="VFP93_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843419.1"
                     /translation="EIIMKLSLTFIGIAFLILGVISLGYQGFTYTQEEKVAEIGSFKV
                     TEQKEKSIPIPPIVGGVSIIIGIILVIQGSRKKP"
     gene            346..1062
                     /locus_tag="VFP93_02020"
     CDS             346..1062
                     /locus_tag="VFP93_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843420.1"
                     /translation="MKLFTKIMVGLLASSFIPSVWALATKFDPTIYEREIYDIGFDDV
                     ELIKSDLRLRSNESVVVKHVLTPGEKRASCENIVLEINQLESITPRLNAYEMFNADRP
                     VLLTDEQVNSLTKSFTNHKDKEWLNDRNTKKYIQIVLMKTGQTNGNTSTNGATSKNGR
                     TSTNGNTSVQQGTSNGNDPMNSTHANGQKNGERNSDVNGRHKNKTFSALTTKDAKKLV
                     EWAIKNKKIVEEDHNIKTAQ"
     gene            1102..1524
                     /locus_tag="VFP93_02025"
     CDS             1102..1524
                     /locus_tag="VFP93_02025"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9843421.1"
                     /translation="MKREASAQWQGALKKGKGTLSTKSKTLNETPYSFTDRFEGNGTN
                     PEELIAAAHAGCFTMALCAILGKEGYTPTALNTKAELSLEQVEGNWTVTNIDLAVEAE
                     ISDIEEQKFEQIAEQAKKDCPISKLLKTNIGLQVTLIR"
     gene            complement(1532..2536)
                     /locus_tag="VFP93_02030"
     CDS             complement(1532..2536)
                     /locus_tag="VFP93_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET9843422.1"
                     /translation="MEVTFLLFIAIVILALIFDYINGFHDAANAIATVVSTKVLSPRV
                     AVIYGALLNFIGALLGTQVATTIGTGLVDTQAVSSEVLLAALLSAIIWNLFTWFKGLP
                     SSSSHALMGSLTGAVAFAAGTHNLHWDGIIYKVLIPMFTSPLMGFVLGYFLMLGLYWL
                     LHKTTLPIINWWFSKIQIVSAGYMAITHGANDAQKTMGIIALAVATYYKSAFHVSEWI
                     IISCAIAMGLGTIAGGWKIIRTVGSKMVKLKPIQGFAAEATGGTILFLTAHFGIPVST
                     THTITTSIMGVGSAQRFSALKLQIVGNIVVAWLLTLPITFFLAGGIFWVLHQILSTTL
                     "
     gene            complement(2549..>3080)
                     /locus_tag="VFP93_02035"
     CDS             complement(2549..>3080)
                     /locus_tag="VFP93_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET9843423.1"
                     /translation="IEEDEENAFKQAREIEMAKQKAKEITYEITEMLCRTLVTPFDHE
                     DIHRLSRGLYKILKISEKAQERLVAFHLRSAQNDFFKLTTNMVEASDVVHSLVISLKT
                     LKDSKSIHDKCALIHNIETNTDKLLNQLTFDLFQHENDIKQILIRREMYGLLESIVDR
                     HRDLANIILEIVLKHS"
BASE COUNT         1070 a          560 c          568 g          882 t
ORIGIN      
        1 gagattatta tgaagctatc tttaactttt atagggattg cttttttaat actgggggta
       61 atttccctgg gatatcaagg ttttacgtat acacaagagg aaaaagtagc ggaaattggt
      121 agttttaaag ttactgaaca aaaagaaaaa tccattccta tccctcctat tgtaggtggg
      181 gtttcgatta ttattggaat tattctcgta attcaaggat cccgcaaaaa accgtaatcg
      241 ttgtaagcta atactctgaa caaaaatata taaaataata aagatttttg ccttttacat
      301 gcatatacac taaaatttaa atacccaatt ttcggagcta aattcatgaa attatttaca
      361 aaaattatgg tcggcttact tgcttcttct tttattccca gtgtttgggc attagcgacc
      421 aagtttgatc ctaccattta cgagcgtgaa atttatgata ttggttttga tgatgttgaa
      481 ttaatcaaat ctgatttgcg attaagatcc aatgaaagtg tggtggttaa gcatgtatta
      541 actcccggtg aaaaaagagc aagttgtgaa aatattgttt tagaaattaa tcaattagaa
      601 agcattacac cgcgactcaa tgcttatgaa atgtttaatg cggatcgtcc tgttttatta
      661 accgatgaac aggtaaattc acttaccaaa tcctttacga accataaaga taaagaatgg
      721 ttaaacgatc gaaacacaaa aaaatatatt caaattgttc ttatgaaaac tggacaaacg
      781 aatggtaata cctcgactaa cggtgctacc tctaaaaacg gtagaacttc aaccaacggt
      841 aatacctcag tgcaacaagg tacttccaat gggaatgatc caatgaatag tacgcatgca
      901 aacggtcaaa aaaatggtga gagaaattct gacgtcaacg ggcggcataa aaacaaaacc
      961 tttagtgcat taaccacaaa ggatgccaag aaattagtag agtgggcaat taagaacaag
     1021 aagattgttg aagaagatca caatattaaa acagcccaat aaattataag gggctttgcc
     1081 ccttaaatac ttgaggtaat tatgaaaaga gaagcaagtg cacaatggca aggggcttta
     1141 aaaaaaggga aaggcacgct tagcacaaaa agcaaaacgc tcaatgaaac tccctattcg
     1201 tttacggatc ggtttgaagg caatggtaca aacccagaag aattaatagc tgcagcgcat
     1261 gcaggctgtt ttaccatggc cttatgtgca atcctcggaa aagaaggtta cacccctact
     1321 gcattaaata ccaaagctga gttgtcgctt gaacaagtgg aaggtaattg gacggtgaca
     1381 aatatcgatc tagctgtaga agctgaaata tccgatatag aagaacaaaa gtttgagcag
     1441 atagcggaac aagcaaagaa ggattgccca atttctaaat tgttaaaaac caatattggt
     1501 ttacaagtaa ccttaattcg ttaaacgaat gttacaaagt ggtactaaga atttggtgta
     1561 aaacccaaaa aatcccgcct gcaagaaaaa aggttatggg cagcgttagc aaccaagcta
     1621 caacaatatt gccaacaatt tgtaacttta acgctgaaaa acgctgcgcc gatcctaccc
     1681 ccataatcga agtagtaata gtgtgcgtgg tgcttacggg aataccaaaa tgggctgtta
     1741 aaaacaagat agttccacct gtggcttccg cagcaaatcc ttgaattggt tttagtttta
     1801 ccattttcga gccaactgtg cgtataattt tccaacctcc agctatggtt cccaagccca
     1861 tcgcaatagc gcaagaaatt ataatccact cagaaacatg aaacgcagat ttatagtaag
     1921 tagctacagc taatgcaata attcccatcg ttttttgagc atcgtttgct ccatgggtaa
     1981 ttgccatgta gcctgcagac acaatttgaa ttttactaaa ccaccagttg ataatcggta
     2041 aagtggtttt atggagcaac caataaagtc ccaacataag aaaataccct aatacaaatc
     2101 ccataagagg agaggtaaac ataggaatta aaactttata aatgatacca tcccaatgta
     2161 aattatgggt tcctgcagca aatgctacag cacccgttaa ggatcccatt aaagcatgtg
     2221 aggaactaga aggtaggccc ttaaaccaag taaacaaatt ccaaataatt gcgcttaaaa
     2281 gtgcagctaa taaaacttct gagctaacag cctgtgtatc aaccaatccc gttcctatgg
     2341 ttgtggccac ttgggtaccg agaagtgccc cgataaaatt taacaaagcg ccatagataa
     2401 ccgcgactct cggagatagc acttttgtcg acaccacagt tgcaatagcg ttcgctgcat
     2461 catgaaaacc attaatatag tcaaaaatca aagcaagtat aacaatggca ataaaaagta
     2521 aaaaggttac ttccatagta ttctcgggtt aagaatgttt taaaacaatt tccaagatta
     2581 tatttgccaa atcccgatgg cggtctacaa tactttctaa tagtccatac atttctcttc
     2641 gaattaaaat ttgttttata tcattttcat gctgaaataa atcaaaggtc aattgattta
     2701 ataatttatc ggtgttggtt tcaatattgt gaattaaagc gcatttatca tgaatcgatt
     2761 tgctatcttt taacgttttt aagctaatta cgagcgagtg cacaacatcc gatgcctcga
     2821 ccatattggt ggttaattta aaaaaatcat tttgtgcgga gcgtaaatgg aatgcaacta
     2881 aacgttcttg tgctttttcg ctaattttta aaattttata taaaccacgt gagagtcgat
     2941 ggatatcttc atgatcaaac ggtgttacta aagtgcgaca aagcatttcg gtaatttcat
     3001 acgtaatttc tttggctttc tgcttagcca tttcaatttc acgggcttgc ttaaaagcgt
     3061 tttcttcgtc ttcttctatc
//
LOCUS       DASTIB010000117         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_707227, whole genome shotgun sequence.
ACCESSION   DASTIB010000117 DASTIB010000000
VERSION     DASTIB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707227"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2448)
                     /locus_tag="VFP93_02040"
     CDS             complement(<1..2448)
                     /locus_tag="VFP93_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET9843424.1"
                     /translation="MGDITKTSSELENQIYDAADGETTVNLTPGQLYVMRRDNRTLVP
                     FQADKIKIAITKAYLAVEGGNAAASRRVHELVEELTTQVTQAVSRHLNSGGVVRVEDV
                     QDQVELTLMRHGLHKVARTYILYREERRVEREKKTKPQNTHTAALQVTLADGSRIPLE
                     QTKLALTVKRACEGLEEVNGDFIVSDTINNIFDGIKVADIRKALIMSARTKIEEEPNY
                     SYVAARLLLDDLHNEALDFLGLPVPETLEEKPKFYPEIFKKYITQGIEFELLSPSLKD
                     FDLDRLGRALLPARDHQFTYLGLQTLYDRYFIHYQGTRFELPQIFFMRVAMGLALEES
                     NKNERAIEFYQLLSSFDYMSSTPTLFNSGTLRSQLSSCFLTTIPDDLAGIYDGMKQNA
                     LLSKYAGGLGNDWTYVRAMGAHIKGTNGKSQGVVPFLKVANDTAVAVNQGGKRKGAMC
                     AYLETWHLDIEEFLELRKNTGDDRRRTHDMNTANWVPDLFMKRVFEDKDWTLFSPDEV
                     PDLHDLYGVAFEEAYIAYEQKAARGKIKNFKVIKALQLWRKMLSMLFETGHPWITFKD
                     SCNLRSPQGHVGVVHSSNLCTEITLNTSVDEIAVCNLGSINLAQHITENGLDLEKLEK
                     TIQTAIRMLDNVIDINYYSVIQARRSNLKHRPIGLGLMGFQDALYQLDIPYASLKAIE
                     FADTSMEAISFFAIKASTDLAQERGTYASYEGSLWSKGILPIDSIQRLAEYRKNYLEL
                     DRSTTLDWHTLRQRVMTVGMRNSNVMAIAPTATISNICGVSQSIEPTYQNLYVKSNLS
                     GEFTVVNPYLVKDLKKLK"
     gene            complement(2693..>2914)
                     /locus_tag="VFP93_02045"
     CDS             complement(2693..>2914)
                     /locus_tag="VFP93_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843425.1"
                     /translation="GSISEASASLMQKVLSWCERQFNAIGKTEEEAEKLALNLVASLQ
                     GISLISLTFNDPKYIGQHGQFLTHWLEAL"
BASE COUNT          865 a          576 c          561 g          912 t
ORIGIN      
        1 tttcagcttt tttaagtctt tcactaaata aggattgaca accgtaaatt ccccggacaa
       61 atttgatttt acatacaaat tttgataagt gggttcaata gattgtgaaa ctccacatat
      121 gttagagata gtcgcggtgg gagcaattgc cataacattg gaattacgca ttcctactgt
      181 cataacacgt tgtcgaagtg tatgccaatc taaagtagta gaacgatcga gttccaaata
      241 gttttttcgg tattctgcca agcgttgaat agaatcaata ggaagaatgc ccttgctcca
      301 cagtgatcct tcataagaag catacgtgcc acgttcttgg gctaaatcgg tagatgcttt
      361 aatagcaaaa aagcttatag cttccatcga agtatcggca aattcaatcg ctttaagaga
      421 agcataaggg atgtctaatt ggtaaagcgc atcttggaaa cccattaaac ctaaacctat
      481 ggggcgatgt tttaaattag aacgtcttgc ttgaataacc gaataataat taatgtcaat
      541 tacgttatcg agcatgcgta tcgcagtttg aatggttttt tctaattttt ccagatcaag
      601 tccgttttcc gtaatatgtt gcgctaaatt aatagatccc aaattacaaa ccgctatttc
      661 atctacagag gtatttaaag tgatttccgt gcataggttc gaactatgga ctacacctac
      721 gtgtccctgg ggtgagcgta aattacagga atctttaaaa gtgatccaag gatgccctgt
      781 ttcaaacagc atgctgagca ttttacgcca taactgcaat gctttaatta ctttaaagtt
      841 tttaattttg ccacgagccg ctttttgttc ataggcaata taggcttctt caaaagctac
      901 cccataaaga tcatgaagat ccggtacttc atcaggagag aatagagtcc aatctttatc
      961 ttcaaacaca cgtttcataa agagatcagg cacccagttg gcagtattca tatcatgggt
     1021 gcgacgtcta tcatcgcccg tgtttttacg aagttctaaa aattcttcaa tatctaagtg
     1081 ccaagtttct aaataggcac acatagcacc tttgcgttta ccaccttgat ttactgcaac
     1141 tgcggtatcg tttgccactt ttaagaaagg aacaacgcct tgggatttgc cattggttcc
     1201 tttaatatgg gcccccatcg cacgaacgta agtccaatcg tttcctaaac caccggcata
     1261 ttttgaaagt agagcatttt gcttcatgcc atcgtagatg cctgccaaat cgtccggtat
     1321 tgtagtcaaa aaacaactag acaattggga acgtaatgtg ccggaattaa ataaagtagg
     1381 ggtagagctc atataatcaa aagaagatag caattgataa aattcaatag cgcgttcgtt
     1441 tttgttggat tcttctagag caagtcccat cgcgacccgc ataaaaaata tttgtggcag
     1501 ctcaaaacgc gtgccttgat aatgaataaa atatctatcg tataaagttt gtaatcccaa
     1561 atacgtgaat tggtgatcgc gtgctggtaa caaagcgcgc cccagtctat cgagatcaaa
     1621 atcttttaag ctgggtgaaa gtaattcaaa ttcaatgcct tgggtaatgt attttttaaa
     1681 tatttcagga taaaatttag gtttttcttc taaagtttct ggaaccggta acccgagaaa
     1741 gtctagagct tcattgtgaa gatcgtctaa caataaacgg gcggccacat aggaataatt
     1801 gggctcttct tcaatttttg tgcgtgcact cataatgagt gctttgcgga tatcggctac
     1861 tttaatccca tcgaatatat tattaatggt gtcagataca ataaaatcgc cattaacctc
     1921 ttctaaacct tcgcatgcgc gtttaacggt aagtgctaat tttgtttgtt caagaggaat
     1981 tcgactgcca tccgctaatg ttacctgtag agcagcagtg tgggtatttt gcggctttgt
     2041 tttcttttct cgttcaaccc gacgttcttc gcgatacaaa atataggttc ttgcaacttt
     2101 atgcagtcca tggcgcatta atgttagttc aacttgatct tgaacatctt ctactcttac
     2161 caccccacca ctatttaaat gtcttgaaac agcttgtgtt acttgggtcg taagttcttc
     2221 taccaattca tgcacgcgtc ttgaagctgc tgcattaccc ccttccacgg ccaaatacgc
     2281 tttcgtaatg gcaattttaa ttttatcggc ttgaaagggt actaaagtgc gattgtcacg
     2341 tcgcataaca tataattgtc cgggtgtcag attaacggta gtttctccgt ctgctgcgtc
     2401 ataaatttga ttttctaact cggaagatgt tttagttatg tcacccatgt tttcccctag
     2461 tactactatt gtttttggag accaacctat gaaccccaac atatagttgc tccaaatctc
     2521 ttatacacaa gatagtgcgc ttttaatctc tttgcaagtg aagaacctgt gggtaagttg
     2581 gggaaaaact gtcaacaatg aaaattttga aaataaaggt gatagaagcg aagaaggggt
     2641 gaaaaagcaa gtagggtttt tcagtatttc tctacttgct ttttagagac gattaaagtg
     2701 cttcaagcca atgggtcaaa aattgaccat gctgaccaat atattttgga tcattaaaag
     2761 tgagagaaat taaactaata ccttgaagac ttgcgactaa atttaaagca agcttttcag
     2821 cttcttcttc cgtttttcct atggcattga actgacgctc acaccaagag agaacttttt
     2881 gcattaagga agcagaggct tcactaatac tacc
//
LOCUS       DASTIB010000118         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_293810, whole genome shotgun sequence.
ACCESSION   DASTIB010000118 DASTIB010000000
VERSION     DASTIB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293810"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..554)
                     /locus_tag="VFP93_02050"
     CDS             complement(54..554)
                     /locus_tag="VFP93_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843426.1"
                     /translation="MPARYEILDDLQDIKDRVVYSIQDFFQDGLSQFLTEIQLGVRDN
                     LYGIKFLVTHPITSVEQIVSSLRHPIKTFKKLSKFASKNPTRYWTNLLLSYLEGRAIT
                     AVAPNLNVLQEFYDLGNDIMPITLRDVLKHDRLILFAIPEEKNQKSEQKSKACALEQN
                     IHRMGF"
     gene            836..>2638
                     /gene="parE"
                     /locus_tag="VFP93_02055"
     CDS             836..>2638
                     /gene="parE"
                     /locus_tag="VFP93_02055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET9843427.1"
                     /translation="MAIREKYTAQSIEVLSGLEPVKKRPGMYTDTTRPNHLAMEVIDN
                     SVDEALAGYASEIFVTLFKDDSCEVRDNGRGMPVDKHEKEKIPGVEVILTRLHAGAKF
                     SNKSYTFSGGLHGVGVSVVNALSERLDVTIARDGKQHTMSFADGIKKTNLKVAGDCAK
                     RETGTTLRFWPNPKYFDSPKFSITKLEHLLEAKAVLCPGLVIHLQIEGQKETKTWVYE
                     DGLASYLLYKTQGNTVLPEIPFIGDFKSEHEAVNWALTWLPEGGELLTESYVNLIPTP
                     QGGTHVNGLRTGLADAMRDFCEMRNLLPRGIKLAPEDTWDTCCYVLSLKMQDPQFAGQ
                     TKERLTSRQSSAFVSGVVKDAFSLWLHQHTKEGEQIAEMIIAHAQNRLRKGKVIARKK
                     ITQGPQLPGKLADCSSSEPLKSELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTW
                     EVEPQEVLASQEVHDIALAIGLDPSQSDLSTLRYGKICILADADSDGLHIATLLCALF
                     LKHFRSLVEAGHVYVAMPPLYRIDVGKDVFYALDEEEKQGILDRINAEKKSGKINIQR
                     FKGLGEMNALQLRETTMSPDTRRLLQLTLGDETNA"
BASE COUNT          841 a          462 c          562 g          773 t
ORIGIN      
        1 ttttatataa attaaatgag cgattctact attcttacag agctttgcaa taattaaaaa
       61 cccattctat ggatgttttg ctctaaagca caagcctttg atttctgttc cgatttttgg
      121 tttttttctt ccggaatcgc aaaaagaata agcctgtcat gctttaaaac atcgcgtaaa
      181 gtgatgggca ttatgtcatt gcctaaatca taaaattctt gcagcacatt taaattgggg
      241 gctactgcgg ttatggctct tccttctaag taagataaaa gtaaatttgt ccaatatcgg
      301 gtaggatttt tgctggcgaa tttgcttaat tttttaaaag tttttatggg atggcgcaaa
      361 ctagatacga tttgctcaac acttgttatt ggatgtgtaa ctaaaaattt tataccataa
      421 agattatcac gaacacctaa ttgtatttcc gttaaaaatt gcgacagtcc atcctgaaaa
      481 aaatcctgaa tactatagac aacgcgatct ttgatatctt gcaaatcatc caatatttca
      541 taacgggctg gcataatctt cctgccaaaa tgtcgcgatt aagtacacgc attatatcag
      601 gggcttttta agatgcgaaa tatctcacat ttgacaattt ttgttattca gcttagattg
      661 tgcgataact aaaagtttgc agatcttgat tcgacactct gagaataatg gccgagtaag
      721 ttatttaatt aaaaatttaa ttatcgttta aactctgaat tgctttcgag gaaacttcaa
      781 ggttaaccta cctgcaataa tttcttcacc gtagtaatca gttaagtatc caattatggc
      841 aatacgtgaa aaatatacag cacaatccat agaagtgtta agtggcttag agccagtcaa
      901 aaaaagaccg ggaatgtata cggacactac acgtcctaat catttagcta tggaagtgat
      961 tgataatagt gtagatgaag ctttagcggg ttacgcgagt gaaattttcg tcaccctttt
     1021 taaagatgat tcctgtgagg tgcgcgacaa cggtcgcggt atgccggtag ataaacatga
     1081 aaaagaaaaa ataccgggtg tagaagtcat tttaacaaga cttcacgcag gagctaaatt
     1141 ttcaaataaa agctatacct tttctggcgg gctgcatggc gtgggtgttt cggtggtaaa
     1201 tgctttgtcg gagcgattag acgtgactat tgcgcgtgat ggcaaacaac acacgatgag
     1261 cttcgctgat gggataaaaa aaacgaatct aaaagttgcc ggtgattgcg ccaagcgaga
     1321 aacgggcact acgctgcgct tttggccaaa tccaaaatat tttgatagtc ccaaattttc
     1381 tataacgaaa ttagaacatt tgttagaagc aaaagcagtt ttatgcccag ggcttgttat
     1441 tcatctacaa atagagggtc aaaaggaaac gaaaacgtgg gtatacgaag atgggcttgc
     1501 atcctattta ctatataaaa cccaaggtaa caccgtttta cctgaaattc catttattgg
     1561 ggattttaaa tctgagcatg aagcagtcaa ttgggctctt acttggttgc ctgaaggagg
     1621 cgaattatta acagaaagtt atgttaatct cattccaacg ccccaaggtg gcacccatgt
     1681 aaatggatta cgcacaggct tagcagatgc catgcgcgat ttttgtgaga tgcgcaattt
     1741 attaccgcgc ggtattaaat tagccccaga agacacctgg gatacctgtt gttatgtttt
     1801 atcattaaaa atgcaggatc cacaatttgc tggccaaacg aaagagcggc ttacttcaag
     1861 gcaatcgagt gcttttgtgt ctggtgttgt gaaagatgcg ttcagtcttt ggctgcatca
     1921 gcataccaaa gagggcgaac aaattgctga aatgataata gcgcacgcgc aaaatcggtt
     1981 gcgcaaagga aaagttattg cgcgcaaaaa aattacccaa ggtccccaat tgccaggtaa
     2041 attagcagat tgttctagca gcgaaccctt aaaaagcgaa ctgtttttgg tggaaggtga
     2101 ttccgcagga ggatcggcaa aacaagcgcg tgatagagaa tttcaagcaa taatgccatt
     2161 acggggaaaa attttaaata cttgggaagt tgaacctcag gaagttttgg catctcaaga
     2221 agttcatgat atagccttag ctattggatt ggatcctagt caaagtgatc tatcaacttt
     2281 acgatatgga aaaatatgta tattggctga tgcggattcg gatggtttac atattgcaac
     2341 cttattatgt gcactatttt taaaacattt tcgatcttta gtagaagcgg gtcatgtata
     2401 tgttgccatg ccacctcttt ataggattga tgtgggcaaa gatgtttttt atgcattaga
     2461 tgaagaagaa aagcaaggaa tcttagatag aataaatgca gaaaagaaaa gcggaaaaat
     2521 taacattcaa cgtttcaaag ggctgggcga aatgaatgcg ctgcaattac gtgaaacaac
     2581 catgtcgccg gatacaagac ggttattaca gttaacgctc ggcgatgaga ctaatgcc
//
LOCUS       DASTIB010000119         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1105551, whole genome shotgun sequence.
ACCESSION   DASTIB010000119 DASTIB010000000
VERSION     DASTIB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105551"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1013)
                     /locus_tag="VFP93_02060"
                     /pseudo
     CDS             complement(1..1013)
                     /locus_tag="VFP93_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(1047..2126)
                     /locus_tag="VFP93_02065"
     CDS             complement(1047..2126)
                     /locus_tag="VFP93_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9843428.1"
                     /translation="MRKIFILNGGSQDDLIALIYLASRPDIKIVGIAVENTGAAHGFE
                     GTKNIAALCNFLGLPSMPIAYSLGQQPISKPYLPFPNYFRQMADNILHNKLDPIYFNN
                     FNNNPNAIELLYITMAQSLEKITILVTGPLTNLAQFFILYPNLVHKIERIVIMGGALN
                     VPGNIQELDPNSVNTQAEWNILADPQAADIIFNSGMDTTLVPLDATNQVPITKSFYQT
                     LSESQNQYVKLIFSLYKNLHDSMGDYVFFNTVFLRSPLAALLLTHPPMARYEALPVCV
                     DIPSGKTHKTYEILRPIQVATKIVEPHAVLTFLQDSITSIHMPIDATQGFQKVLSFKE
                     PQPQRIHAKIPIEKSKPRIRSNTFS"
BASE COUNT          686 a          377 c          443 g          712 t
ORIGIN      
        1 tgcagggtat agtgtggcga aaacgaagca ttggaagatc cttgtatttt tgtcgggttg
       61 ttatttaatc tttttgctac caggtcacac aattcttgaa gaaatttttc aggttgtaag
      121 attttagaga ctacttggat atctctttcg ttattattct catacttctt agttttccca
      181 gagataaaat ccacttgtaa agaaagcgag caatattccg cgagttcagg cttcagacag
      241 agcattgccg acatagtatc ccagaaataa atttttgcaa aaaatgcatc tcccatttca
      301 tcttttaagt cttttaaaaa ggcatgcatc aatacgtgat cgggattatt ggataatact
      361 aaggagtaat aaaaatgttg ggtaataggc acttggttgg tagcatctaa agggactaaa
      421 gtaataggta tatgagaggc aaaaacaata tctgcggctt caggatccgc tgcaatatta
      481 cattcagctt cactgaatac aagagtttct tgaatatcca aaacgttgcc ggcaacctag
      541 atagcacctc ccataataaa aatgcgacca atttttttgg ttaattcagg atatttttgg
      601 ataaaacggg caatattggt taaaggacct gtagctaaaa tttctgtcga ttcgtttttc
      661 tcaacgatac ttctaataag ttcaatagca ttgttggtaa taagaggtgc aggatggtgg
      721 ggtatatttt tatttttcaa tacttcattg gcttgatggc ggatataatc aggaaaacgc
      781 aaaaaattat tgttacgact tatcggtaag tcactgccat aagcaatggg tacgttcaac
      841 cctaaataat aacaaaaatc cgctaaattt ttcgtacctt gaataccatc agcttctcct
      901 gttgtagcaa gcgttacagc ggctatttca atttccggat cgcaagccat aactaacagt
      961 gcaatgagat catcataagc gccatcattg tcgctaataa aaattcgttt catgcaatct
     1021 ttcctttgct ttagaataag aattatttac gaaaacgtgt tggatctaat acgtggtttt
     1081 gatttttcta tcgggatttt agcgtggata cgctgaggtt gcggctcttt aaaagagagc
     1141 actttttgaa agccttgtgt ggcatcgata ggcatgtgaa tgctggtaat cgaatcttgt
     1201 aaaaaggtta atactgcatg gggctcaaca atttttgtcg caacttggat tggacgtaga
     1261 atttcatagg ttttatgggt cttgccgcta ggtatatcca cacatacagg caatgcttcg
     1321 tatctcgcca taggagggtg agttaagaga agggcagcta gtgggctgcg caaaaaaacc
     1381 gtattaaaaa aaacgtaatc ccccatacta tcgtgtaaat ttttatacag agaaaatata
     1441 agttttacat attggttttg cgattcactt aaagtttgat aaaaggattt ggtaatagga
     1501 acttggttgg tagcatctaa aggaacaagt gttgtgtcca ttccagagtt gaaaataata
     1561 tctgcagctt gtggatccgc aagaatattc cattctgctt gcgtattcac agaattagga
     1621 tctaattctt gaatattgcc tggcacattc agcgcgccac ccataattac aatacgttcg
     1681 attttatgca ctaaatttgg gtaaagaata aaaaattgcg caagattcgt taaaggacct
     1741 gtaaccaaaa tggtaatttt ttctaggctt tgcgccatcg ttatgtatag cagttcaatc
     1801 gcgtttggat tattgttaaa attgttaaaa taaattggat caagtttgtt gtgcaaaata
     1861 ttatctgcca tttggcgaaa ataattggga aaggggagat aaggcttact tataggttgt
     1921 tgccctaagc tgtaggcaat gggcatactc ggtaaaccta aaaaattaca aagggcagct
     1981 atattttttg tcccttcaaa cccgtgtgcg gctcctgtat tctctacggc aataccaaca
     2041 attttgatat ctggtctgct ggctaaataa ataagcgcaa taagatcatc ctgagaacca
     2101 ccgtttaaaa taaagatttt gcgcataaac tccctttttc ataaggttga ttattgggtt
     2161 acttcaaaca tagaacttat agattgttct tcacttattc gttttataac ctgagaaa
//
LOCUS       DASTIB010000120         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_730602, whole genome shotgun sequence.
ACCESSION   DASTIB010000120 DASTIB010000000
VERSION     DASTIB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730602"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1236
                     /locus_tag="VFP93_02070"
     CDS             352..1236
                     /locus_tag="VFP93_02070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9843429.1"
                     /translation="MKYSSHFAMQDSIIQLEPIIRIGFFFLFFAVIVGIEKKFTWRPI
                     FASLKERFFKHFCLLLISTFLVRLVFPVVLVELAIKVQQTNQVSLFQLPELSGLPFAV
                     QFCVSFLLLDLIMYWQHRIFHRYGLLWRMHLAHHSDSILDLTTGIRFHPLEMLFLVGL
                     RMLGVILIGPPILAVLLFEIVFNAFSMFVHSNTQLPPRIEKILRKLIVTPGMHRVHHS
                     DIPQERDANFGIIFSFWDKMLGTYRAYSHMGDYKIIVGLEEFRDPKEKTLFAILLQPF
                     YLQKKRKKKPQLRKMMRN"
     gene            complement(1263..>2016)
                     /locus_tag="VFP93_02075"
     CDS             complement(1263..>2016)
                     /locus_tag="VFP93_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843430.1"
                     /translation="RDIIPDELPKDAGWLQTGVHWYFYTVESIPSAIVHFGDKVVRTK
                     TPAEYAAPAWYEYGEYCADKIFSSAESFANLLASSKDLFPAMQVAFEEMKKQGLKQTV
                     LNGIQHTTEFAAWCSNELAVFTKPLTRNGYELAKWTYNGCKDSSWQYGEKVYEITDGW
                     ISPKLAIALSAAAMSGLACLYLKYKLRRIPAPLVVNNNQQIIQPQPAPLLLSAQVSQA
                     QALPLPGQPDPSQIQAQPQQVPQPAAVPAPQP"
BASE COUNT          564 a          359 c          397 g          696 t
ORIGIN      
        1 attcgataag tgtaaagatg caggatgcga ttattagcac accaaatgcc gaattcttca
       61 tgaaagtggg attaatacgt tctttttgtt ttgagttttt cataattacc accgttattt
      121 tcgttataac tttgatttca aaacaatctt tttcaagaat gcttaggaag gaaaatcaaa
      181 ggtgtttgaa aaaacaggcg acaaacttca aactcctgag tcacccagct tatgttactt
      241 gacatagaga taacaacata agaactgcgc gtctattaca acaaatgcat ttttattctg
      301 caagtagata tttagtggga agcgcctgtt aaagctgtta aacttaattt attgaaatat
      361 tcttctcatt ttgccatgca agattccatc atccaattag aacccattat tcgaatagga
      421 ttttttttct tattttttgc agtgattgtg ggaattgaga aaaagtttac ttggcgtcct
      481 atttttgcct cgcttaaaga acgttttttt aaacattttt gcttgttgct catttcgaca
      541 tttttagttc ggttagtatt tcctgttgtt cttgtcgaac ttgcaataaa agtgcaacaa
      601 actaatcaag ttagtttgtt tcaattacct gaattaagtg ggttgccgtt tgcggttcaa
      661 ttttgtgttt cttttctttt gctcgattta attatgtatt ggcagcatcg aatttttcat
      721 cgttatggat tattatggcg catgcatttg gcacatcata gtgattctat tttagatcta
      781 actacaggaa ttcgctttca tccattagaa atgctatttt tagttggatt gcgcatgcta
      841 ggagtaattt taattggtcc tcctatttta gcagtcctat tatttgaaat tgtatttaac
      901 gctttttcta tgtttgtgca tagtaatacg caattaccgc cgcggataga gaaaatttta
      961 agaaaattaa tagtaacacc tggaatgcat agggtgcatc actcagatat accgcaagaa
     1021 cgtgatgcca attttggaat tattttttcc ttttgggata aaatgcttgg aacctatcgt
     1081 gcttattcgc acatgggtga ttacaaaatt attgttgggt tagaagaatt tcgtgatccg
     1141 aaagaaaaga ctttatttgc tatccttttg caaccttttt atcttcaaaa gaaacgcaaa
     1201 aaaaaaccac agcttcgaaa aatgatgcgg aattaaaaag ccggaaaaat ccggctttca
     1261 tgttaaggct gcggggctgg gactgcagca ggttgaggaa cttgttgtgg ctgtgcttgg
     1321 atttgactgg gatccggttg gcctggtagc ggcaaagctt gcgcttgcga aacttgtgca
     1381 gatagcaata ggggcgcagg ttgaggttga ataatttgtt gattattatt aaccactaac
     1441 ggtgcgggta tgcgacgcag tttatatttt aagtataaac aagctaaacc agacatagct
     1501 gcagcgctta gtgctatcgc taattttggc gaaatccaac catccgttat ctcgtatact
     1561 ttttcaccat attgccatga agaatcttta cagccattgt aagtccattt agctaattca
     1621 tagccgtttc gtgtgagagg tttcgtaaat actgcaagtt catttgaaca ccaagcggca
     1681 aattccgtcg tgtgctgtat accgtttaaa acggtttgtt ttagtccttg ctttttcatt
     1741 tcttcgaatg cgacttgcat tgcgggaaat aaatctttgc tgctcgctag taaattagca
     1801 aaagactctg cagaagagaa aattttatct gcgcaatatt cgccatattc ataccatgca
     1861 ggtgcggcat attccgcagg agtttttgtt cttactactt tatccccgaa atgcacaatt
     1921 gcactgggta tactttccac cgtgtaaaaa taccaatgga cccctgtttg tagccaacct
     1981 gcatcctttg gcagttcatc agggatgata tcgcgt
//
LOCUS       DASTIB010000121         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1041530, whole genome shotgun sequence.
ACCESSION   DASTIB010000121 DASTIB010000000
VERSION     DASTIB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041530"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..272)
                     /locus_tag="VFP93_02080"
     tRNA            complement(179..272)
                     /locus_tag="VFP93_02080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Ser,seq:gct)
     gene            complement(318..530)
                     /gene="csrA"
                     /locus_tag="VFP93_02085"
     CDS             complement(318..530)
                     /gene="csrA"
                     /locus_tag="VFP93_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HET9843431.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEISVHR
                     EEIYKRIQQEKGEAAGTAESSSETTE"
     gene            complement(574..1818)
                     /locus_tag="VFP93_02090"
     CDS             complement(574..1818)
                     /locus_tag="VFP93_02090"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9843432.1"
                     /translation="MTLIVQKFGGSSVKDLACIQHVAGKIIETRNQGHSVVVVVSAMM
                     GETDRLDKMAKALMVKPTPRELDVLLSTGEQVTIALLTLALNQMDCAAKSYTGVQVRI
                     LTDSAFTKARILDIDDTKMRQDLAKGLVVVVAGFQGVDIHGNITTLGRGGSDTTAVAL
                     AAALDADECQIFTDVDGVYTTDPRMVPSARRMPRITFEEMLELASLGAKVLQIRSVEF
                     AGKYNVPLRVKSTFQEGPGTLITYEERGMEQPVVSGIAFNRDEAKLIIRSVPQVPGIQ
                     ARILGPISDANIEVDMIIQNQGIDGLTDFAFTVHRRDYLKTLEILQGICEKLGAKEVS
                     GDDKIAKISLVGVGMRSHAGIASTMFKVFGEEGINIQLISTSEIKVSVIMDEKYLELG
                     VRALHAAFRLHETPIEEKSKAA"
     gene            complement(1887..4481)
                     /gene="alaS"
                     /locus_tag="VFP93_02095"
     CDS             complement(1887..4481)
                     /gene="alaS"
                     /locus_tag="VFP93_02095"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET9843433.1"
                     /translation="MKLQALRSLFLEYFEKNDHSVIPSSSLVPYNDDTLLFTNAGMVQ
                     FKDVFLGREKRTYTRAASSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIQLAWNFLTKELKIPAEKLWVTVFQNDDEAADIWLKEIGINPQQFARCGEKDNFW
                     MMGDTGPCGPCSEIYYDHGKEIAGGPPGSIDAEGDRYIEIWNLVFMQYNRAPDGSLTA
                     LPKPSVDTGMGLERLSAVMQGVHNNYEIDLFQNLIQHAAQIVETHDATSQSLKVLADH
                     IRSCSFLILDGVLPSNEGRGYVLRRILRRAIRHGYKLGAKQTFFYKLVEALVREMGDA
                     YPDLKKNQSHIEKVIRKEEEQFAKTLDQGIKLLEQELRTLQSKTIPGSIVFKLYDTYG
                     FPMDLTADYAREMGLSIDEHGFEQAMSVQRARARASHQFQSDYHKVEQDLGESAFSGY
                     ATHADEGTVIALLKDGNPTDILQEGESGGVILHNTPFYAEAGGQVGDQGRLQNDNVLF
                     TVEDTQKSQKAHVHLGKLSQGTLRIGDKVHALVDENLRQRTRLNHSATHLLHAALRKI
                     VGPHVQQKGSLVAPDRLRFDFTHYEPLSSDQIEAIEDCVNDQILANSLVQTDEMPVEE
                     ALASGAMALFGEKYGEKVRVLKMGNGFSVELCGGTHVNRTGDIGLLKILYETGIAAGV
                     RRIEAITGNNATQYLRETQKQLSTICGLLKVDRLKASDRVAQMVKKQRELEKENERLS
                     LKAASAQGQDLTESARQIGSLKVLSLQIPGDPKMLRGLMDDLKNKLGSGVIVLAVVHE
                     QKISLIAGVTKDYTNKISAGELVNFVATQVGGKGGGRADVAQAGGNAPENLTNALESV
                     YEWVEQKI"
     gene            complement(4545..>4887)
                     /locus_tag="VFP93_02100"
     CDS             complement(4545..>4887)
                     /locus_tag="VFP93_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET9843434.1"
                     /translation="AATILQLKDNKYLDEARFIGCIIRARIGQGLGPLRILAEFKEHQ
                     IPPSKVENHEEWQKISWFDNAFEIKERRFGKITCADFKQQQKQKLFLQRRGFTLDQIR
                     HAFSETSSAEG"
BASE COUNT         1308 a         1125 c          875 g         1579 t
ORIGIN      
        1 ctctatccaa ctgagctacg ggcgcactag gattgcgatt atgtccactt atagttggca
       61 tgtcaaatta ttgattacca actatttaag acatcacact aaggaaaacc atattctgcc
      121 tgcccaaaaa acaaaaccct acaccttcat ttcgttgaag gtttagggga agataatttg
      181 gcggagagag agggattcga accctcgatg gagtttataa gccccatact cccttagcag
      241 gggagcgcct tcagcctctc ggccatctct cctttccgtg gccgcaatgg tatcacgatg
      301 aaaggtatgt gaaaagtcta ctcagtagtt tctgaagaag attctgcagt tccagctgct
      361 tcaccttttt cttgctgaat acgcttataa atttcttcac gatgtaccga aatttctttc
      421 ggagcattca cacctattcg aacttggtta ccttttaccc caagaaccgt tactgtgact
      481 tcatcaccaa tcattaatgt ctcgccgaca cgtcttgtca aaatgagcat gttaatctcc
      541 ttactgcggt aggagccaac cctttaaaag gaattaggcc gccttactct tctcctctat
      601 aggtgtttca tgcaatcgaa acgctgcgtg taaagctctt acacccagct ctaagtattt
      661 ttcgtccatt atgaccgaga ctttaatctc ggaggttgaa attagctgga tattaattcc
      721 ttcttcgcca aatactttaa acatagtact ggctattcca gcatgagaac gcatgccaac
      781 tcctactaga gaaatttttg ctattttatc gtctccactt acttccttgg cgcctaattt
      841 ttcacatatc ccttgtaaaa tttctaaagt ctttaaataa tctcgcctat gcaccgtaaa
      901 agcgaaatct gttaggccat ctattccttg attttgaatg atcatatcta cttcaatatt
      961 tgcatcactg atagggccta atattctcgc ttgtattcca ggcacttgtg gaacgcttcg
     1021 aataattagt tttgcttcat cccggttgaa tgcgatacct gatacaactg gttgttccat
     1081 ccctcgctcc tcgtatgtaa tcaacgtacc tggcccttct tgaaaagtag attttactct
     1141 caatggcaca ttatatttgc cagcaaattc aacagaacga atttgtaaaa ctttcgctcc
     1201 taaacttgcc aattccaaca tctcctcgaa ggttattcta ggcattcgac gtgcactagg
     1261 aaccatacgt ggatcggttg tgtaaacacc atctacgtcc gtaaatatct gacactcgtc
     1321 tgcatctaac gctgctgcta atgctaccgc cgttgtgtct gatccgcctc ttcccaaagt
     1381 cgttatattt ccatggatat ctacgccttg aaatcctgca accactacca ccaaaccttt
     1441 ggctaagtct tgacgcattt ttgtatcgtc gatatctaaa atacgcgctt tagtaaaagc
     1501 actatccgta agaattcgga cttgaacacc cgtatatgac ttggcggcac aatccatttg
     1561 atttaaagct aaagtgagca acgctatcgt tacttgctca cccgttgaaa gcaacacatc
     1621 taactcacgt ggagtaggtt ttaccatcaa cgctttagcc attttgtcta atctatctgt
     1681 ctcacccatc attgcggaaa caacaacaac cacggaatgt ccttggtttc ttgtttctat
     1741 gattttgcct gccacatgtt gaatacaggc taggtcttta acagaagatc caccaaactt
     1801 ttgtactatt aaagtcattt taagcgttcc attgcactgc gcaattctaa catgaactgg
     1861 attttccacc aataatgatt aatggtctaa attttctgtt caacccattc gtatactgat
     1921 tctaatgcat ttgttaaatt ttcaggggca ttaccgcctg cttgggcaac atctgcacgt
     1981 ccgccccctt taccacccac ctgagtagcg acaaaattaa ctagttcacc agctgatatt
     2041 ttattcgtgt aatctttagt tactccggca attaaactaa ttttttgttc atgcacgacg
     2101 gcaagaacta taacgccact tcccaatttg ttttttaaat catccattaa accacgtagc
     2161 attttgggat cacccggaat ttgtaaactg agtactttaa gacttccaat ttgacgcgcc
     2221 gattcggtta aatcttgtcc ttgtgcactt gccgctttta aacttaaacg ttcgttttct
     2281 ttttccagct ctctttgttt tttaaccatt tgcgctacgc gatccgatgc ttttaagcga
     2341 tccactttca ataatccaca aatagtgctt aattgttttt gtgtttctcg caaatattgg
     2401 gttgcgttat taccggtaat cgcctcaatt cgacgaaccc ctgccgcaat gcctgtttcg
     2461 taaagaattt ttaataaacc aatatcgccc gttcgattca catgggttcc accgcaaagt
     2521 tccacagaaa aaccattccc catttttaga acccgtactt tttctccata tttttctcca
     2581 aataaagcca tagctcccga agctagcgct tcttccactg gcatttcatc ggtttgcact
     2641 aaggaattgg ctaaaatttg atcgtttacg caatcttcga tcgcttcaat ttgatcagac
     2701 gataaaggtt cataatgggt aaaatcaaac cgtaatcgat cgggagcaac taaagagcct
     2761 ttttgttgaa cgtggggtcc tacaattttt cgaagtgctg catgtaataa atgggttgca
     2821 gagtggttaa ggcgggtgcg ctggcgcaaa ttttcatcga ctaacgcatg gaccttatcc
     2881 ccgatacgta atgtaccttg ggaaagttta cccaaatgta catgtgcttt ttgcgatttt
     2941 tgcgtatcct caacggtaaa caaaacgtta tcgttttgca aacgcccttg atcgcctact
     3001 tgaccacccg cttctgcata gaacggcgta ttatgtaaaa taacaccacc gctttctcct
     3061 tcttgcaaaa tatccgtcgg atttccgtct tttaaaagtg ctataacggt gccttcatcg
     3121 gcatgggtag cataaccaga gaaagcactt tctcctagat cctgctccac tttatgataa
     3181 tcgctttgga attgatggct tgctcttgct cgagcgcgtt gcactgacat tgcttgctca
     3241 aacccatgct catcaatact taaacccatc tcgcgtgcat agtctgccgt taaatccatg
     3301 gggaatccat aggtatcgta taatttaaaa acgatagatc ctggaatggt tttgctttgt
     3361 aaggtgcgta attcttgttc gagtaatttg atgccttgat ctaacgtttt cgcaaattgc
     3421 tcttcttctt tacgaataac tttttctata tgtgattgat ttttctttaa gtctggatag
     3481 gcatctccca tttctcgcac caaagcctcg actaatttat agaaaaaagt ttgcttagcg
     3541 cctaatttat agccatggcg aatagcgcgt cgtaaaatac gtcgcagtac atagcctcgc
     3601 ccttcattgg aaggtaagac accatctaag ataagaaagg aacaagagcg aatgtgatcg
     3661 gccaaaactt ttaaggattg tgaagttgca tcgtgtgttt caacaatttg tgctgcatgt
     3721 tggattaaat tttggaataa atcgatttca taattattat gaacaccttg cattacagcc
     3781 gataaacgct caagccccat gccggtatcc acggagggtt ttggtaacgc ggtgagtgat
     3841 ccatcgggtg cccgattgta ttgcataaaa acgaggttcc agatttcaat atatctgtct
     3901 ccttctgcat cgatcgatcc gggagggccg cccgctattt ctttgccatg atcatagtaa
     3961 atttccgaac aagggccaca aggtccggta tctcccatca tccaaaagtt atctttttct
     4021 ccacagcggg caaattgctg tggattgatg cctatttctt ttaaccaaat atccgcagct
     4081 tcgtcatcgt tttgaaatac cgttacccat agtttttcgg cggggatttt taattctttt
     4141 gttaagaaat tccatgctag ttgaattgct tctcttttaa agtaatcgcc aaagctgaag
     4201 ttacctaaca tttcaaaaaa agtgtgatgt cttgctgtat aacccacatt ttctaaatcg
     4261 ttgtgtttgc caccagcacg cacgcaacgt tggctacttg cagcccgtgt ataggtacgt
     4321 ttttcacgtc ctaaaaaaac gtctttaaat tggaccatgc ccgcattggt aaaaagtagc
     4381 gtgtcatcgt tataaggcac taaagaactt gagggaatta cactatgatc atttttttca
     4441 aaatattcta agaataggct tctaagcgct tgtaacttca tttttttacc tagtaagtca
     4501 gtttttaaaa ttctatcgcg atcactttaa aaaagataga acgattaacc ttctgctgaa
     4561 gatgtttctg aaaatgcgtg ccttatttga tctaaggtaa atcctctgcg ctgtaaaaaa
     4621 agtttttgtt tttgctgctg tttaaaatct gcacacgtta ttttgccaaa acgccgttct
     4681 tttatttcaa aagcgttatc gaaccaggat attttttgcc attcttcatg gttttccacc
     4741 ttggaagggg gaatttggtg ctctttgaat tctgctaata tccgtaaagg ccccaaacct
     4801 tgtcctattc gtgcacgaat tatacagcct ataaaacgtg cttcatctaa atatttatta
     4861 tcttttaatt gtagaatcgt tgcagca
//
LOCUS       DASTIB010000122         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_271185, whole genome shotgun sequence.
ACCESSION   DASTIB010000122 DASTIB010000000
VERSION     DASTIB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271185"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFP93_02105"
     CDS             complement(<1..966)
                     /locus_tag="VFP93_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9843435.1"
                     /translation="MNEINWHLSYLPRAKILIVDDRPENIFALKKILADMNTLQIDVA
                     HSGEEALYKTIETQYALVILDVQMPKMDGFTVAKYLREYEPTKDLPVIFVTAINKDSD
                     HIRLGYKLGAVDYLFKPVNPIVLRSKVNVFISLYNAAQRSEKDRKDVEQILNALAESI
                     IGIDVNYNIHYVNRAGLQILGISSVDPEKSVFDYLQLFSHGTQINITDTLLSRLKEKH
                     SIEIPEVIDIVNAKGDMYTIEFDVHQVFDYEQLLRGMIFTMRDITISCQLSEKLQYQA
                     LNDLLTRLPNRIFFTERLKTAIAAGIREKQTHALLFFDLDGFKKIN"
     gene            complement(963..1805)
                     /locus_tag="VFP93_02110"
     CDS             complement(963..1805)
                     /locus_tag="VFP93_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9843436.1"
                     /translation="MKTNTAHDLLEAELLLEAIYRVHGYDFRSYARASILRRIQDQVQ
                     RSKIQHISDLISKVIYDEDFFHDMLENLPIKVTEMFRDPFFYKALRSKVIPHLQSYPF
                     IKIWSAGCATGEEAYSLSILLKEEELLHKAHIYATDINLRALKTAKEATYPVETMVKN
                     RINYQKFEGKSDLENYYFKDYHHVRIAEYLRNKIIFSEHSLVTDGIFGEMNVIVCRNV
                     MIYFDHTLQNKVFTLLCNSLCYQGFLCLGDKETLNFSAIAPYFKEIDPYAKIYQKKVS
                     GYIT"
     gene            complement(1774..5904)
                     /locus_tag="VFP93_02115"
     CDS             complement(1774..5904)
                     /locus_tag="VFP93_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9843437.1"
                     /translation="MKLNSIKAYILGGLGFVGFLFVCSILYAIFEIRSITKLSDKLNE
                     ETTPAVTNGLKLLANVNDSIAQIRGWMMSPNEVFKIKRQDTWSEINANITRLETLSNK
                     WTDSQLALLKGIKTKLFQLKLYQEDIEQLSHTPDNNPSLNFYANTMVPLIDSLTQEAK
                     SIQISEVEIKNFDPAPFNRILNEYQQTFEFLSLNLQAYLSGKSDSSRLKVVNLWAKQK
                     DLLNQLKNYERYLTPVAKVSINKLELGMQRLESLQNEYVLKEEKIVTNKAEQLFLTQG
                     TKLAHEISTDINSMISLQNKVRSNERINIHQRAADGRVHHIILLVLAFSIVVATCVVL
                     LRKIIVPVKRTIDIAKNVSQGSFDVDINLQGTSEINQLGFALSQMVDYLKRATRVTSE
                     VAKGNFEEKMIVVSEKDQLGPSVNEMIDSLKRFTERTNNENWVKTGQAKMAELLRSEE
                     DLTQLGSNVIKFLSEYVKAAVGTFYLITENNKLKLHASYAYHYRKGSKTEFSIGESLV
                     GQAALEKSTIVVNEIPKNYIKIQSGIGESEPKSLIISPLIINKRLKGVIELGTFEPLN
                     ELQRTFIESTSEAIAISIASLQDREHLRNLVDQTKAQAEELQQQSEELKASNEELEEQ
                     AEALKASEEQLREQSEQLRVTNEELEEKTESLEKQQKEIEKKNMELQRKQQEVTQKAQ
                     ELENSSRYKSEFLANMSHELRTPLNSLLILSEVLMENNEKNLNEEQLESIKVIYNGGK
                     DLLNLINDILDISKVEAGKLKVQYDVVEIKQIIEDIKKQFDPISMQTDIPFKIVLSKK
                     LPETMVTDPLRLEQILKNLLANAFKFTHKGQVELKICHANENVQYKTESLKNTDTMAF
                     SVKDTGVGIAKENLQRIFEAFQQVDGTTSRKYGGTGLGLTISNKIADILGGEIQVKSK
                     EGKGSEFTLYVPIKATQNLQTQEEIRITEEVESATQFFQETTAQQTFRSVAQPLIDGN
                     AKGDTHRTVLIIEDDQDFAQLLSKVAKEKGYIPIHATNGTSGMQMAQEKIPSAIILDL
                     GLPDKDGLEILESLKNHNGTKNIPIHICSCKDAQTSALKMGALGSLLKPARLNELYGM
                     FNKIELALQDKLKEILIVEDNPSSQHAITALFKNQNINIVTAECGMDALRLLSNRNFD
                     CMILDYRLPDMTGLELLKQFSQSEKYHTQPTIIYTGKDLTQDEYMELNKYTNNIILKV
                     SNSRQRLYEEASLFLHSLPGKGEDKTKKTKLAHIGEKLKNKRVLLVDDDMRNVFALST
                     ALKKFNLDLTIAGNGKMALDKLATNDPFDLIIMDIMMPEMDGYEAMKHIRADARFNHI
                     PIICLTAKAMAGDEKKCMDAGANDYLTKPVDLNLLTSMIKLWLEQSHENQYGSRSA"
BASE COUNT         1764 a         1195 c         1039 g         2222 t
ORIGIN      
        1 attaattttt ttaaacccgt ccaaatcaaa aaataacaat gcatgggttt gcttttctcg
       61 aatacctgca gcaattgcgg ttttcagtcg ttccgtgaag aaaatacgat tgggcaatcg
      121 ggttaacaaa tcgtttaacg cttggtattg taatttttcg gataattggc aagagatagt
      181 gatatcgcgc atcgtaaaaa tcataccgcg caataactgt tcgtaatcga atacttggtg
      241 cacatcaaac tcgatcgtgt acatatcgcc cttagcattc acaatatcga tcacttcagg
      301 aatttcaatg gaatgctttt ctttcaatcg agacaaaagg gtatcggtga tattaatttg
      361 cgtgccatga ctgaaaagct gcaaataatc aaacacgctt ttttcgggat ctacactaga
      421 aattcctaaa atttgtaggc ctgcacggtt gacataatga atgttataat tcacatcaat
      481 tccaataatg gattcggcta atgcgtttaa tatttgttcc acatccttgc gatctttttc
      541 tgatctttgg gctgcattat ataaagatat aaatacatta actttgcttc gtaaaacaat
      601 aggattcacg ggcttaaata aataatcgac cgcacccaat ttgtaaccca gacgaatatg
      661 atcgctatct ttattgatag cggtaacgaa aatcaccggt aagtctttgg ttggttcata
      721 ttccctgaga tatttggcta cagtaaatcc atccattttt ggcatttgca cgtcgagaat
      781 gaccaatgca tactgagttt caatcgtttt ataaagtgct tcttcgcccg aatgtgccac
      841 atcaatttgc aaggtattca tatcggctaa aattttcttt aatgcaaata tattttccgg
      901 tctatcatcg acgatgagta ttttggcgcg cggtaagtaa cttaagtgcc aatttatttc
      961 attcatgtaa tatatcctga aacctttttt tgataaattt ttgcataagg atcaatttct
     1021 ttaaaatagg gagcaatagc agaaaaattg agcgtttcct tgtcgcctaa acataaaaat
     1081 ccttgataac ataagctgtt gcaaagcaag gtaaatactt tattctgcaa agtatgatcg
     1141 aaataaatca tcacatttcg gcaaacaata acgttcattt caccaaaaat accatcggta
     1201 actagactat gttccgaaaa aataatttta ttccttaaat attccgcaat acgtacatgg
     1261 tggtaatctt taaaataata attttctaaa tcgcttttac cctcgaattt ttgataattg
     1321 atcctattct tcaccatcgt ttcaacagga taagttgcct ctttagctgt ttttaatgcg
     1381 cgcaaattta tatcggtggc ataaatgtgt gctttatgca ataattcttc ttctttaaga
     1441 agaatagaaa gtgaatacgc ctcttcacct gtagcgcatc ctgcgctcca aattttaata
     1501 aaaggataac tttgtaaatg tggtataacc ttggatcgta atgccttata aaaaaaagga
     1561 tcacgaaaca tttccgttac tttaatggga agattttcta acatatcatg gaaaaaatct
     1621 tcatcgtaaa ttacttttga aataagatcg gatatatgct gaatttttga acgctgaact
     1681 tgatcttgta ttcgacgtaa aatggaagct cgcgcatagc ttcgaaaatc atagccatgc
     1741 acgcgataaa tcgcttctaa taacaactct gcctcaagca gatcgtgagc cgtattggtt
     1801 ttcatgcgat tgttctaacc ataatttaat catagaagta agcaaattaa gatctacagg
     1861 ttttgttaaa taatcgttcg cgccagcgtc catgcatttt ttttcatctc cagccatagc
     1921 ttttgctgtt aaacaaatta tgggaatatg gttaaaacgc gcatcggctc ttatatgctt
     1981 catggcttca tacccatcca tttccggcat cataatatcc atgataatca aatcaaacgg
     2041 atcgttcgtt gccaatttat ccaaagccat tttgccattt cccgctatag tcaaatctaa
     2101 attaaatttc tttaatgctg tagaaagagc aaatacattc cgcatgtcat cgtccactaa
     2161 taacacacgt ttgtttttta gcttttctcc aatgtgtgcc agttttgttt ttttagtttt
     2221 gtcctcgcct ttaccaggta aactatgtaa gaataatgaa gcttcttcat agagtcgctg
     2281 tctcgaatta cttaccttta atataatatt gttggtatat ttattaagtt ccatgtattc
     2341 atcttgcgtt aaatctttac ctgtataaat aattgtgggt tgtgtgtggt atttttcact
     2401 ttgactaaat tgctttaaca actcaaggcc tgtcatatcg ggtaaccgat aatctaaaat
     2461 catgcaatcg aagtttctat tcgacagcaa gcgcaaagca tccataccgc actctgccgt
     2521 gacaatatta atattttgat ttttaaataa tgccgtaata gcatgctgcg agcttggatt
     2581 atcttcgaca attaaaatct cttttagctt gtcttgtagg gctagctcta ttttattaaa
     2641 catcccataa agttcattga gtctcgcagg ttttaataag ctacccaatg cgcccatttt
     2701 caatgccgaa gtttgggcat ctttacatga acaaatatga ataggaatat ttttggttcc
     2761 attatggttt tttaaacttt caagaatttc taaaccatct ttatccggga gccctaaatc
     2821 gagaataata gcgctcggga ttttttcctg cgccatttgc ataccggatg taccgttagt
     2881 tgcatggata ggaatatacc ctttttcttt tgctacctta cttaacaatt gtgcaaaatc
     2941 ctgatcatct tcaataataa gtaccgttcg atgtgtatct ccttttgcat ttccatcgat
     3001 taatggttgt gctaccgatc gaaaggtttg ttgagcagtc gtttcttgaa aaaattgggt
     3061 agcactttcc acttcttctg taatccgtat ctcctcttgc gtttgaagat tctgtgttgc
     3121 ctttataggg acatataggg tgaattcact gcctttgcct tctttacttt ttacttgaat
     3181 ttctccacct aatatatctg caattttatt cgaaatggta agtcctaacc cagtacctcc
     3241 atatttccta ctggttgttc catctacctg ttgaaaagcc tcaaaaatgc gttgtaaatt
     3301 ctctttcgcg ataccaactc ctgtgtcttt cacggaaaat gccatggtat cggtattttt
     3361 taaactttct gttttgtact gcacattctc gttagcgtgg caaattttaa gctccacctg
     3421 acctttatgc gtgaatttaa atgcgttggc gagcaaattt ttcaaaattt gttccaagcg
     3481 cagcggatcg gttaccatgg tttccggcaa ttttttactc aatacaattt taaatggtat
     3541 gtcggtttgc atagaaattg gatcaaattg ttttttgata tcttcaataa tttgctttat
     3601 ttctacaaca tcatattgaa cctttaactt gcccgcttct actttggata tgtccaagat
     3661 atcattaatt aagtttaata aatctttacc accgttataa ataactttga tagactctag
     3721 ttgctcttcg tttaaatttt tctcattatt ttccattaac acttcggata atatcaaaag
     3781 actattgaga ggcgttcgta attcatgtga catattggcc aaaaactccg atttatatcg
     3841 gcttgaattt tcaagctctt gggctttttg tgtcacttct tgttgtttac gttgtagttc
     3901 catattcttt ttttcaattt ccttttgttg tttttctaga ctttcggttt tttcttcgag
     3961 ttcttcattg gttacgcgca attgctcact ttgttcgcgc aattgctctt ccgatgcttt
     4021 taacgcttca gcttgttctt cgagttcctc attggaagcc tttaactctt cgctttgttg
     4081 ttgtaactct tcggcttggg ctttggtttg gtctacaaga ttgcgcaaat gttccctatc
     4141 ttgtaatgag gctattgaga ttgctatcgc ctccgatgtc gactcaataa atgtacgttg
     4201 caattcgttt agaggttcaa aagtgcctaa ctcaatcaca ccttttaatc ttttattaat
     4261 aattaaagga gaaataataa gagatttagg ttctgattcc ccaataccgg actggatttt
     4321 aatataattt ttcggtattt cattgactac aatggtactt ttttctaagg ctgcttggcc
     4381 gactaaactc tcaccaatgc taaattctgt tttactacct ttgcgataat gataggcata
     4441 agaagcatgt aacttcaatt tattgttttc tgtgattaaa taaaaagtac ccactgccgc
     4501 tttaacatat tcacttaaaa attttataac attgctccct aattgggtga gatcctcctc
     4561 agagcgcaat aattccgcca tctttgcctg tccggttttt acccaatttt cattattcgt
     4621 tctctcggtg aaacgtttaa ggctatctat catttcattg acggaaggcc ccaattgatc
     4681 cttttcgctc actacgatca ttttttcttc aaaatttcct tttgcaactt cgctagtgac
     4741 gcgagtggca cgctttagat aatcgaccat ttgtgataaa gcgaagccca attggtttat
     4801 ttcgctagtt ccctgcaaat tgatatctac atcaaaacta ccttgtgaaa catttttagc
     4861 gatgtcaatg gttcttttta cgggaacaat aatttttctt aacagtacta cgcatgtagc
     4921 taccactatg gaaaaagcaa gtactaacag aatgatatga tgtactctcc catctgctgc
     4981 tctttggtgt atatttattc tttcgttaga acggacttta ttttgcaaag atatcattga
     5041 attaatatcc gttgaaattt catgcgccaa tttggttcct tgtgttaaga acaattgctc
     5101 tgctttgtta gtcacgattt tctcttcctt caagacatat tcgttttgca gggactctaa
     5161 acgttgcatt cccaattcca gtttattgat agaaaccttg gcgacaggtg ttaaataacg
     5221 ttcgtaattt ttcaattgat taagcaggtc tttttgcttt gcccacaggt ttacaacttt
     5281 aagcctagaa gagtcacttt tcccggacaa gtatgcttgc aaattgagtg ataaaaattc
     5341 aaatgtttgt tgatattcat tgagaattct attgaagggt gctgggtcaa aatttttaat
     5401 ttctacttcc gaaatttgaa tcgattttgc ctcttgtgta agagaatcga taagcggtac
     5461 catagtattc gcataaaaat tgagacttgg gttattatcg ggagtatgac ttagctgttc
     5521 aatatcttct tggtagagtt ttaattgaaa taatttcgtt ttaatgcctt tcaacaacgc
     5581 tagttgagaa tcagtccatt tattagataa tgtttctaat ctcgtaatat tcgcgttaat
     5641 ttcactccat gtatcttgtc tttttatttt aaaaacctca ttgggactca tcatccagcc
     5701 acgaatttgc gcgatagaat cgttaacatt ggcgagtagc ttaagaccat tcgtcactgc
     5761 aggtgtggtc tcttcgttca atttatctga aagtttcgtg atggatctaa tctcaaagat
     5821 ggcgtacagt atgctgcata caaaaagaaa accaacgaac ccgagacctc ccaaaatata
     5881 ggcctttatg ctattgagct tcatatcatt tgtcctttca tggatcttgc taagaaaatc
     5941 actgagtttg ccttcttaat taaattatag ggaagttttc gtaaacctga gatgggacat
     6001 aatttcagct taaagattga taattcgacc aattctgatc aaaaaacacg gaacaatttt
     6061 agagagatgt taaaatttaa aaataaaaaa agttgcgatc gcacgcagat ctcgatacct
     6121 acgtgataaa tgcttaagag ttatagatat cttaccggtc tatttcggtt ttttttcttt
     6181 accaacactt ggtggcattg tgcgacgcat gcttccttcc
//
LOCUS       DASTIB010000123         7559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_807590, whole genome shotgun sequence.
ACCESSION   DASTIB010000123 DASTIB010000000
VERSION     DASTIB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807590"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFP93_02120"
     CDS             complement(<1..679)
                     /locus_tag="VFP93_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843438.1"
                     /translation="MAKYVAYMIAPSHRNGALFFATDALNNQEKHIQDFLQLQEGELI
                     ATFVEVGGSQRHRHRWPQLQNAVKHCKNHHAHLLIAEIRNLTHNESFANIISGFLTST
                     NSHPFEIHCLDQPFITRETFGAIVKHANEQRKYHGKLIKEGLHRAHAHPGNPRAFEVI
                     SKVNKPKIDNAITFSLLMMPVIHDYQQKGFSQRKIVQLLNEEGYHAPEGGNWVLSQLQ
                     KVLDRMKF"
     gene            complement(817..1656)
                     /gene="map"
                     /locus_tag="VFP93_02125"
     CDS             complement(817..1656)
                     /gene="map"
                     /locus_tag="VFP93_02125"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012220633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9843439.1"
                     /translation="MGAILSCSVIVFKEFPLIHIKSPDEIEKMRVAGRLAAEVLEMIE
                     PYVKPGITTSALKDICHDYIVNTQKAIPAPLNYSGSGGTPFPKSICTSVNHQVCHGIP
                     SEKILKNGDIINIDVTVIKEGYHGDTSKMFGVGEISTQASRLMRVTRECMILGIQQVK
                     PGAFLGDIGYVIETHAHQHRYSVVHEYCGHGIGRFFHEEPQILHYGKQKTGLILEPGM
                     TFTIEPMINAGKRHVKLLPDSWTVVTKDRQLSAQWEHTLLVTETGYEIFTLRKDELDL
                     LNK"
     gene            1891..2637
                     /gene="rpsB"
                     /locus_tag="VFP93_02130"
     CDS             1891..2637
                     /gene="rpsB"
                     /locus_tag="VFP93_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9843440.1"
                     /translation="MANITMRALLEAGVHFGHHTRYWDPKMGPFIYGSRNRVHIINLD
                     KTLPLFKQALNFVSRIAGRNGKILFVGTKFQAGKVIKEHAERCGMPFVNFRWLGGMIT
                     NFKTVKQSIKRLKDLEAMRDDGTLSKLIKKEAMMRMREHEKLDRSLGGIKDMGGLPDA
                     LFVLDVGHEHIAIREANKLGIPVIGIVDTNNSPDGIDYVIPGNDDALRSIELYVSQIA
                     DTIIDARNAQQASVRMEDSEYVEMDDDESK"
     gene            2717..3601
                     /gene="tsf"
                     /locus_tag="VFP93_02135"
     CDS             2717..3601
                     /gene="tsf"
                     /locus_tag="VFP93_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9843441.1"
                     /translation="MADISATDVKNLRERTGAGMMECKKALVEANGDMALAEELIAKA
                     GSRRVQKTADRTAAEGLILAQVNPDQKSGALIEVNCETDFVTRATDFKEFCQKLVHVV
                     AQAKNDNLESIKSMPIDNQQTVEEARQALVAKIGENIQFRRAQIVHAHQGVVGSYVHN
                     DRIGVLVVISTNNEKLAKDLAMHTAAMRPQFVKPEDVPQTIVEKEKEIFIARAAESGK
                     PANIVEKMIEGQVKKFVNEICLIGQPFIKDPDKQISQLLKEHNTTVESMIRFEVGEGI
                     EVVKKSFQEEVMEQARRS"
     gene            3603..4319
                     /gene="pyrH"
                     /locus_tag="VFP93_02140"
     CDS             3603..4319
                     /gene="pyrH"
                     /locus_tag="VFP93_02140"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9843442.1"
                     /translation="MALKYRRILLKLSGEALQGEESFGISPKVLDRMAIEVGELIKLE
                     IQVGMVIGGGNLFRGQALSKAGLGRISADHMGMLATVMNAIAMRDALERAGFSTRVMS
                     AIPMSGVVDHYDRRKAMHHLEQGRVVIFSAGTGNPLFTTDSAASLRAIEIDADILLKA
                     TNVNGIYSSDPARDPMATLYERITFSKVLEKELGVMDLAAFCQCRDHNVPIRVFNMNH
                     AGALLRICQGGNEGTLVEQG"
     gene            4322..4879
                     /gene="frr"
                     /locus_tag="VFP93_02145"
     CDS             4322..4879
                     /gene="frr"
                     /locus_tag="VFP93_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9843443.1"
                     /translation="MLENIKTDADRRMKKTIATLEDELGKLRAGRAHPSLLEGITVIS
                     YGNETPLNQVASVGVDGALMLTIKPWDKQMIPAIEKAIRISDLGLNPATSGDIIRVPL
                     PPLSEERRKELIKKVKAEGEAAKVSVRNIRRDANSTIKDLLKEKEISEDDERKAEEFI
                     QKLTDKYVQEIDQILAKKEKDLMVI"
     gene            4902..5660
                     /gene="uppS"
                     /locus_tag="VFP93_02150"
     CDS             4902..5660
                     /gene="uppS"
                     /locus_tag="VFP93_02150"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET9843444.1"
                     /translation="MKKSYAHYRGEKASQNRLPQHVAIIMDGNGRWAKQRFLPRTAGH
                     RQGVETVRQMVKASINHGIRVLSLFAFGKENWKRPVKEVRTLFRLLFLTLKKEINDLH
                     KQGVRLQIIGDKKALAPMILDAIQTAELQTHQNQTLLLNIFVNYSGRWDIQQALKKTM
                     GNPQVNEANCEYLFEKSLCLSDVPEPDLFIRTAGVQRLSNFMLWDLAYTELYFTQTLW
                     PAFSLQDFEEALNFYTNQERRFGLISEQVENTHA"
     gene            5653..6450
                     /locus_tag="VFP93_02155"
     CDS             5653..6450
                     /locus_tag="VFP93_02155"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9843445.1"
                     /translation="MLKARVLTAIVLIPFVIAGIFFFPDFFPWIAGFVTLVAAKEWDN
                     LLEFSWLEQGAFLTFILSMLFLFAEIELQTLMPYSLGFWIIMAGLVLGYKPGFGGKTS
                     KPAGTFLGTFLLIPFWISFLWFSEQPSWFILSFFLLVWTADTAAYFGGKKFGKTKLLP
                     HVSPNKTWEGFFCGAVAVGILGNILFFSGVLEGGPSLAQSYGGWIFLLFCIFLASVVG
                     DLFESLVKRIQGAKDSGGLLPGHGGVLDRIDSLLAATPIVVFLCGSS"
     gene            6451..>7559
                     /gene="dxr"
                     /locus_tag="VFP93_02160"
     CDS             6451..>7559
                     /gene="dxr"
                     /locus_tag="VFP93_02160"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET9843446.1"
                     /translation="MVNKICLLGATGSIGLNTLDIIEQYPDHFEVFALSAYAQVDKCF
                     DLIVKFSPKVVVMVDENAAYLLQDKVRQHALSCEVLAGAQALESIVSARDVDTVVAAI
                     VGAAGLRPILQAAKAGKRILLANKEALVMAGTLLMQEIEQSNASILPVDSEHNALFQC
                     MPTHYRIGIKPESVEKIYLTASGGPFLHLPLEAFATITPAQAIKHPKWKMGPKISVDS
                     ATLMNKGLEVIEAHFLFQMPPHEIDVLLHPQSIVHSLVQYEDGSLLAQLGAPDMRIPL
                     SFCLHWPDRKPNRAPKLDFCTMQELQFLQPDPIRFPCLNLAYLALRHGGTAPVVLNAS
                     NELAVEAFLRGTITFLDIASIVARTLESISHVEID"
BASE COUNT         2279 a         1371 c         1668 g         2241 t
ORIGIN      
        1 tgaatttcat acgatcgagt actttttgta attgactcaa tacccaatta ccgccttcgg
       61 gtgcatgata gccttcttca tttaataatt gcactatttt gcgctgagaa aaaccttttt
      121 gttgataatc atgaatcaca ggcatcatca gtaatgaaaa ggttatagca ttatctattt
      181 tgggtttgtt gactttgctt atcacttcaa acgcgcgtgg attgccgggg tgcgcatgcg
      241 cacgatgtaa gccttcttta atgagtttac catgatactt gcgttgctca ttcgcatgtt
      301 tcacgatagc gccaaacgtt tcacgtgtta taaagggttg gtcaagacaa tgaatttcaa
      361 aaggatgcga atttgtactc gttaaaaatc cactaataat attagcaaac gattcattat
      421 gcgtaagatt gcggatctct gcaattaata aatgcgcatg atggtttttg caatgcttta
      481 ctgcattttg taattgaggc caacgatgac gatgccgttg acttccacca acttccacga
      541 aggtagcgat gagttcgcct tcctgtagct gtaaaaaatc ttgaatatgc ttttcttgat
      601 tatttaacgc atcggtcgca aaaaaaaggg caccattccg atgggaaggc gcaatcatat
      661 aggcgacgta ttttgccatg tgtttgccta cacgcgtaaa attagttgaa ttactaagac
      721 ctagtatagg aaattctttg taagttgacc agacaaatgc aagatcaagc tgtaaaatta
      781 tccaaccaaa tgaataaaat aaggtttatg cttaatttat ttattgagaa gatcaagttc
      841 atctttgcgt aaagtgaaaa tttcataacc cgtttcagtt accaacaaag tatgctccca
      901 ttgggccgat aattgtcgat ctttggtgac aacagtccaa gaatccggca acaatttaac
      961 gtgacgtttt cctgcgttga tcataggttc aatggtaaaa gtcatgccag gttctaggat
     1021 taatccggtt ttttgttttc cataatgcaa aatttgcggc tcttcgtgaa agaatcgacc
     1081 gatgccatga ccacaatatt catgtaccac cgaataccga tgttgatggg cgtgggtttc
     1141 gattacatag ccaatatccc ctaaaaaagc gccgggtttt acttgttgaa tgcctaaaat
     1201 catgcattcc cgagtaacac gcataagtcg tgaggcttgg gtggaaatct ctccgacacc
     1261 gaacattttg ctcgtatctc catggtaacc ctctttaata accgtgacgt caatattaat
     1321 gatgtcacca ttttttaaaa ttttttcact cggtatgcca tgacatactt gatgatttac
     1381 agaagtacat atcgacttag gaaatggtgt gccacccgaa ccagaataat taaggggggc
     1441 tggaatagct ttttgtgtat ttacaatata atcgtgacaa atatctttta gtgcgctagt
     1501 ggtgatacct ggcttcacat acggctcaat catttcaaga acttcggccg caagtcgccc
     1561 agcaacacgc attttttcta tttcatcggg agatttaata tgaatcaatg ggaactcctt
     1621 gaatacaatg actgagcagc ttagaatcgc acccatagcc tggtgctatc ttacgtcttt
     1681 ttatgttttg tgggaagctg caaagttaac tcaggttcct cggaagtaaa aagttgttgt
     1741 agatgcccat ctatggtata aaccgcgcgg aggagtggcg ttcactcttc gcaataactt
     1801 atttatcaca cacatatatc gacacaataa ccggggtgcc ctttagggta ggttattggg
     1861 atatatggag gattaaccct aggagttaaa atggcaaata ttactatgcg tgccttgctt
     1921 gaggcaggtg tgcactttgg acatcatacc cgttattggg atccaaaaat gggccctttc
     1981 atttatggtt cgcgtaaccg tgttcatatc ataaatcttg ataaaacttt accattattt
     2041 aaacaagcgt taaattttgt ctcaagaatt gcaggtcgca atgggaaaat tttatttgta
     2101 ggcaccaaat ttcaagccgg caaagttatc aaagagcatg cagaaagatg tggtatgcct
     2161 tttgtaaatt ttcgttggtt aggtggaatg ataaccaact ttaaaacagt taaacaatct
     2221 atcaaacgct taaaagattt agaagctatg cgtgatgatg gaaccttaag caaactcata
     2281 aaaaaagaag ctatgatgcg catgcgtgaa catgaaaaac ttgatcgtag cttaggtggt
     2341 attaaagaca tgggcggttt gccggatgcg ttatttgtgt tagacgtggg gcatgaacat
     2401 attgcgatac gtgaagcaaa taaattaggt attcctgtga tcggtattgt ggatactaat
     2461 aatagtccgg atggcattga ttatgtaatt ccaggaaatg acgatgcttt acgttctatc
     2521 gaattatacg tatcacaaat tgctgatact attatcgatg cacgtaatgc ccaacaagca
     2581 tcagtcagaa tggaagatag cgaatatgtt gaaatggacg atgatgaaag caagtaatcg
     2641 aaatttcctt gtacgataac ttcattgttc atcaatgggg gctttgcccc ctttatttta
     2701 atttatgagg attagcatgg ctgatatatc tgcaaccgat gtaaaaaact taagagagcg
     2761 taccggtgct ggcatgatgg aatgcaaaaa agctttggta gaagcaaatg gcgatatggc
     2821 attagccgaa gagctcatcg caaaagctgg ttcacgtcga gttcaaaaaa ccgcggatcg
     2881 tacggcagct gaagggctca tattggcaca ggtaaatccg gatcaaaaat ctggagcact
     2941 cattgaagtg aattgcgaaa ccgattttgt aacccgtgct accgatttta aagaattttg
     3001 tcaaaaacta gtacatgttg tagcgcaagc aaaaaatgac aacttagaat ccataaaatc
     3061 tatgcccatc gataatcagc aaacggtaga agaagcccgt caagcgctgg ttgcgaaaat
     3121 tggtgaaaat attcaatttc gtcgagcaca aattgtgcat gcacatcaag gcgtagtagg
     3181 ttcgtatgtg cacaatgatc gtattggcgt gttggtggtt atttcaacga ataacgaaaa
     3241 attggcaaaa gatttagcca tgcatactgc cgctatgcgc ccgcaatttg ttaaacctga
     3301 agatgttccg caaacgatcg ttgaaaaaga aaaagaaatt tttatagcac gtgcagcgga
     3361 gtcgggtaag cctgcaaata ttgtagaaaa aatgattgaa ggccaagtga aaaaatttgt
     3421 gaatgaaatt tgcttaattg gtcagccttt cattaaagac cccgataaac aaattagcca
     3481 actcttaaaa gaacacaaca ctaccgttga aagcatgata cgatttgaag taggtgaagg
     3541 gattgaagtg gtgaaaaaga gctttcaaga agaggtaatg gaacaagcgc gcaggagctg
     3601 aaatggcatt aaagtatcgt cgtatattgc ttaaattaag tggcgaagct ttacagggag
     3661 aagaatcttt tggcatctct ccgaaagttt tggacagaat ggccatagaa gtaggggaat
     3721 tgataaagtt agagattcaa gttggcatgg taataggcgg tggcaatttg tttcgaggtc
     3781 aagctttatc caaggcagga ttaggacgaa tctctgctga ccatatggga atgctagcta
     3841 ccgtaatgaa tgctatagca atgcgcgatg ctttagaaag agcaggattc tctacgcgcg
     3901 taatgtcagc aatccccatg agtggggtgg tggatcatta tgatcgcaga aaggctatgc
     3961 accatctaga acaaggtcgt gtagttatat tttcggcagg tacaggtaat ccgctcttta
     4021 ccaccgattc tgctgcaagt ttgcgcgcta tcgaaatcga tgcagatatt ttactcaaag
     4081 cgacaaatgt caatggaatt tattcgtccg atcccgctcg agatcccatg gcaacacttt
     4141 atgagcgtat tactttcagt aaagttttag aaaaagaatt gggtgtgatg gatttggcag
     4201 cattttgtca atgccgggat cataatgtgc ctattcgtgt gttcaatatg aatcatgccg
     4261 gtgcattatt aagaatttgc cagggtggga atgaaggtac attggtagaa caaggttaag
     4321 catgttagaa aatatcaaaa ccgatgcgga tcgccgcatg aaaaagacaa tcgcaacact
     4381 agaagatgaa ttaggaaaat tgcgcgcagg gcgcgcgcac ccttctttat tagaaggcat
     4441 tacggttatt agttatggta acgaaactcc cttgaatcag gttgcctctg taggcgtcga
     4501 tggtgctctt atgcttacca tcaaaccctg ggataaacaa atgatccccg caatagaaaa
     4561 agcgatacga atctccgatc taggattaaa tccagcaaca tcgggcgata tcatacgcgt
     4621 tcctttgcct cccctgagtg aagaaagacg caaagagctt ataaaaaagg tgaaagcgga
     4681 aggtgaagca gcaaaagtat cagtacgtaa tattcgccgc gatgccaatt ctactattaa
     4741 agatttgctt aaagaaaaag aaataagtga agacgatgag agaaaagcgg aagaatttat
     4801 tcaaaaatta acggataaat atgtccaaga aatcgatcag attcttgcca aaaaagaaaa
     4861 ggatttaatg gtcatttaac ctttctaggt aaagacagcc agtgaaaaaa tcgtatgccc
     4921 attatagggg agaaaaagca agccaaaatc gattgccaca acatgtggcg attattatgg
     4981 atggcaacgg tcgctgggct aagcaaaggt ttttaccaag aactgcagga catcgtcaag
     5041 gtgttgaaac agttcgccaa atggtaaagg cttccattaa ccatggtatt cgcgttttaa
     5101 gtttgtttgc ctttggcaaa gaaaattgga aacgtcctgt taaagaagtg cgtacgttat
     5161 ttcgattatt gttccttacg ttaaaaaaag aaattaacga tttacataag caaggggttc
     5221 gcttacaaat tattggcgat aaaaaagcgc ttgctcctat gattttggat gcgattcaaa
     5281 ccgcggaatt acaaacgcat cagaaccaaa ctttactact aaacatcttt gtaaattact
     5341 ctgggcgctg ggatattcaa caagctctaa aaaagaccat gggaaatccg caagttaatg
     5401 aagccaattg cgaatattta tttgaaaaat ctttatgttt atccgatgtg cctgagccgg
     5461 atttgttcat tcgaactgct ggggtacaac gactaagtaa ttttatgcta tgggatttag
     5521 cctataccga gctttatttt acgcaaaccc tttggcctgc tttctctttg caagactttg
     5581 aggaagcact taacttttat acgaaccaag aacgacgctt tggtttaatt agtgaacagg
     5641 tggaaaatac ccatgcttaa agcgcgtgtt ttaacagcga ttgtattgat tccatttgta
     5701 attgctggga tctttttttt tcctgatttt tttccatgga ttgcgggatt tgtaacactg
     5761 gttgcagcca aggagtggga taatcttttg gagttttctt ggttagaaca aggtgcattt
     5821 ttaacgttca tactaagcat gctgttcctt tttgctgaaa ttgaactaca gacacttatg
     5881 ccttattcgc taggattttg gataattatg gcgggtcttg tcttagggta taagccggga
     5941 ttcggaggaa aaacttcaaa acccgcggga actttcttag gaaccttctt attgatccct
     6001 ttttggatca gttttttgtg gtttagcgaa caaccttcct ggtttatttt aagttttttt
     6061 cttctcgttt ggactgcaga cacagcagcc tattttggtg gaaaaaaatt tggcaaaact
     6121 aaattattgc cgcacgtaag tcctaataaa acatgggaag ggtttttttg tggagctgtt
     6181 gcggtaggta tattgggcaa tatcttattt tttagtggcg tcttagaggg tggcccatcc
     6241 ttggcgcaat catacggtgg gtggattttt ttactatttt gcattttctt ggcaagtgtc
     6301 gtaggtgatc tattcgaaag tcttgtaaaa agaatacaag gagcaaaaga cagcggaggt
     6361 ttgttgccgg gacatggtgg ggtgttagat cgtatcgata gtttattagc tgcgactcct
     6421 atcgtagttt ttttatgtgg aagtagctaa atggtaaata aaatatgcct tttaggtgct
     6481 accggatcca taggacttaa cactttggat attattgaac aatatccaga tcattttgaa
     6541 gtttttgcat taagcgctta tgcgcaggtc gataaatgtt ttgatcttat cgtgaaattt
     6601 tctcctaaag ttgtagtaat ggttgatgaa aatgccgctt atttgttaca agataaagtt
     6661 cgacaacatg cattatcgtg tgaagtttta gcaggggcac aagccttaga aagcattgta
     6721 agcgctcgtg atgtcgatac cgttgttgca gccattgtgg gagctgcggg cttacgtccg
     6781 attttacaag cagctaaagc gggaaaaaga attttattag ccaataaaga agctctggta
     6841 atggcaggca cgttattaat gcaagaaatc gagcaaagta atgcttctat tttaccggtt
     6901 gacagcgaac ataatgcgct ttttcaatgc atgccaacac attatcgcat tggaattaaa
     6961 cccgaaagtg ttgagaaaat ttatttaacc gcgtcgggtg ggcctttttt gcatttgccc
     7021 ttggaggcat ttgccactat aaccccagct caggcgatta aacacccaaa atggaaaatg
     7081 ggacctaaaa tttctgtgga tagtgcaacc ctgatgaata aaggtttgga ggtaattgaa
     7141 gcacattttt tgtttcaaat gcctccgcat gaaattgacg ttttattaca tccgcaaagt
     7201 atcgtgcatt ctttagtgca gtatgaagat ggttcgttat tggcgcaact cggtgcacca
     7261 gatatgcgaa tccctttgag cttttgctta cactggcccg atcgcaaacc taatcgtgcc
     7321 ccaaagttgg atttttgcac catgcaagaa ttacaatttt tacaacccga tccaataaga
     7381 ttcccctgct taaatctagc ctatttggca ttgcgtcacg gtggaactgc tcctgtggtt
     7441 ttaaatgcga gcaatgaact tgccgttgaa gcttttttgc gaggaacgat taccttttta
     7501 gacatagctt ctatcgttgc acgcacatta gaatccattt cacatgttga gattgatag
//
LOCUS       DASTIB010000124         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1326813, whole genome shotgun sequence.
ACCESSION   DASTIB010000124 DASTIB010000000
VERSION     DASTIB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326813"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..834
                     /gene="panC"
                     /locus_tag="VFP93_02165"
     CDS             40..834
                     /gene="panC"
                     /locus_tag="VFP93_02165"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9843447.1"
                     /translation="MLIVESVEALQEYREKIGSKTVGFVPTMGNFHLGHLSLMEKARK
                     HHDVVIVSIFVNPTQFDDSGDFLHYPRTLEQDIQHATSKNVDMVFCPTFEALYPDAFT
                     VHVEESLISTTLEGKMRPGHFKGVLTIVLKLLNLIKPHVLYLGEKDYQQYRVIKKMID
                     AFFLNTKISLEATVRAHDGLALSSRNSKLTSAQRQIAPHFFQILSSIQDKEKAKHALT
                     QQGFRVDYLEDYHEKRLAAVRLGDIRLLDNIDLSSIANLVECNEDA"
     gene            827..1666
                     /gene="murI"
                     /locus_tag="VFP93_02170"
     CDS             827..1666
                     /gene="murI"
                     /locus_tag="VFP93_02170"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9843448.1"
                     /translation="MRDPKSAIGVFDSGVGGLTVLRELVKMCPNENFMYLGDTARLPY
                     GTKSKETVIQYALSAAKILLEHNIKCLIVACNTASSVALPALQKAFPHIPVFGVIEPG
                     AKACCEHRQSGKIVVLATESTTRWHAYQKAIHQLNPSLEVIEWACPLFVPLVEEGWTS
                     GPLVKEIVKEVLAPILNMPMEMLVLGCTHYPILKDVIAEVIPNTTIIDSASTTAQELL
                     QLLKVNKLENNSPQRGTLQFMATDGLERFSRIANRFLNLNLPQEKIALVSHPENHYQS
                     KTG"
     gene            complement(1648..>2292)
                     /locus_tag="VFP93_02175"
     CDS             complement(1648..>2292)
                     /locus_tag="VFP93_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843449.1"
                     /translation="SNGNAAIFPIILMQTLGAQVMHWIFTKMHSSIIPLSRLQSLYFF
                     LGITCAFSILFGFLHNLSANIEFMFAYYLSFLLLTPVCLIWDHDTLKRKNFLSKHKIG
                     LALNLMFLFCCGSLLFAYSTLYSLLLFIGILLCVIAGYVMCGQWGGVILGMQLSIFLI
                     AIPEMPMYEIWENSIDISILQVSICLSVILGLSFSTFRKETYFKNYLRHHPVLL"
BASE COUNT          785 a          418 c          439 g          650 t
ORIGIN      
        1 tattaaaatt caagacatgt agtaccatac gagaataaca tgcttatagt ggaatcagta
       61 gaagctcttc aggaatatcg cgaaaaaatc ggatccaaaa cagtagggtt cgtcccaacc
      121 atgggaaatt ttcacttagg gcatttatcc ttaatggaaa aagctagaaa acatcatgat
      181 gtggttatag taagtatttt tgtaaacccg acgcaatttg acgattccgg ggatttttta
      241 cattatccaa gaacgctaga acaagatatc caacacgcca ccagcaagaa tgtggacatg
      301 gtattttgtc cgacctttga ggctttgtac cccgatgcat ttacggtgca tgtagaagag
      361 agtcttatct ccaccaccct ggaagggaag atgcgaccgg ggcattttaa aggtgtattg
      421 actattgttt tgaaattgtt aaatttaatc aaaccccatg ttttatactt aggcgaaaaa
      481 gattatcaac aatatagggt aatcaaaaaa atgatcgatg cttttttctt aaacacaaaa
      541 atttctttag aggctacggt tcgagctcac gatggtttag cattgagttc gcgcaatagc
      601 aagctcacat ctgcgcaaag acaaatagca ccgcattttt ttcaaatttt atcttctatc
      661 caagataaag aaaaagcaaa acatgcgctt actcaacaag gatttagggt agattatctg
      721 gaggattatc atgaaaaaag attagctgca gtgcgcttag gtgatattcg tttactagat
      781 aatatagatt tgtcctccat tgctaattta gttgagtgta atgaagatgc gtgatccgaa
      841 atcggctata ggtgtttttg actcgggtgt aggcggttta accgttcttc gcgaattagt
      901 aaaaatgtgt cccaatgaaa attttatgta tttaggcgat acggcacgtt taccctacgg
      961 tacaaaaagt aaagaaaccg ttattcaata tgcattaagt gccgctaaaa ttctgcttga
     1021 acataatata aaatgtttaa tagtggcgtg caacacagcc tctagtgtag cgttaccggc
     1081 cttacagaaa gcttttcccc atattcctgt ttttggtgtc atagaacctg gggcaaaggc
     1141 ctgttgcgaa cataggcaaa gcggtaaaat tgtggtgctt gcgacagaaa gtacgactcg
     1201 atggcatgct taccaaaaag caattcacca actaaatccc tctctggaag taatagagtg
     1261 ggcatgccct ttatttgtac ctttggtaga agaaggctgg acgagtggtc cactggtcaa
     1321 agaaattgta aaagaagttt tggctcctat tcttaatatg ccgatggaga tgcttgtgtt
     1381 aggatgtacg cattatccaa ttttaaaaga tgttatcgca gaagttattc cgaatacgac
     1441 gataatcgat tcagcttcca ctacagcaca agaattatta cagcttctaa aagtgaacaa
     1501 attagagaat aatagtcctc aacgaggaac tctccaattt atggcaacgg atggactgga
     1561 gcgtttttcc cgaatcgcca atcgtttttt aaacctaaac ttgcctcaag agaagatcgc
     1621 tcttgtctca cacccggaaa atcattatca aagtaaaacg ggatgatgac gcaagtaatt
     1681 tttaaaatag gtttctttcc taaaggtaga aaagcttaaa cctagaataa cacttaaaca
     1741 aatcgatact tgaagaatgc taatatcgat agaattttcc catatctcat acataggcat
     1801 ctcaggtatt gcgattaaaa aaatacttag ttgcatgcct agtatgacgc caccccattg
     1861 tccacacatt acataacccg ctataacaca caataaaatt ccaataaata ataataggct
     1921 atacagagtg gagtaggcaa agagtaaact gccacagcaa aataaaaaca ttaaattgag
     1981 tgctaatcct attttgtgtt tcgataaaaa attcttacgt tttaaagtat cgtgatccca
     2041 aatcaaacaa acaggcgtta ataaaagaaa acttaaataa taggcaaaca taaattcgat
     2101 attcgccgat aaattatgta gaaacccaaa aagaatacta aaagcgcagg taatgcctaa
     2161 aaagaagtat agcgactgta gcctacttaa cggaataata gaactatgca ttttggtaaa
     2221 aatccaatgc ataacttgtg cgccaagcgt ttgcataaga attattggaa aaattgctgc
     2281 atttccattg ct
//
LOCUS       DASTIB010000125         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_285645, whole genome shotgun sequence.
ACCESSION   DASTIB010000125 DASTIB010000000
VERSION     DASTIB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285645"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFP93_02180"
     CDS             <1..476
                     /locus_tag="VFP93_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843450.1"
                     /translation="AMTAIQLLLLGAVALTVTAGLFAVARFSAHHATIPVHPHIRVHH
                     NPAPQVRVHHNPAPQVRVHHNPAPHVRVHHNPTPHIRKHPVNMGRPAHPNPTVPVRSA
                     YKAHFNKHSANLQKIPTVAASTISRPPVTTAFRSNMHHSSPAHPVHGFRATKHRA"
     gene            complement(507..1190)
                     /locus_tag="VFP93_02185"
     CDS             complement(507..1190)
                     /locus_tag="VFP93_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843451.1"
                     /translation="MRLIFFKSVKWIQLAFLGTAISFQIFAAEQSSENLYKYQDENGI
                     EIISTSLPGEAANRGYTVISPRGNVIEVVPPKPSDEEVAKSLKEEQKKVEFEKQKEEE
                     LHKKELEAKKDIMLLKMFGNVQDIERSRDEKLSSIKVQEDITKDNLERIKLQLQKAQE
                     ISKIHQDRNQPVPPQIEETIKDSQFQIEENTKFLIRKTTEKKQIHDQYEKLIERFNEI
                     KVQDSSSVE"
     gene            complement(1221..1865)
                     /gene="pyrE"
                     /locus_tag="VFP93_02190"
     CDS             complement(1221..1865)
                     /gene="pyrE"
                     /locus_tag="VFP93_02190"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9843452.1"
                     /translation="MLLDYQIEFIEFALLHNVLRFGSFTLKSGRHSPYFFNTGAFHGG
                     TPLLQLGRFYAKTLQQHAINFDLLFGPAYKGIPLATATSIALANLGLEKQVCFNRKEI
                     KSHGEMGHLMGAPLHGDVVIIDDVITSGLAKKEAIELIHQTKARVAGIVIAFDRQEPG
                     ENKQTAVSELIAMYQIPVCSIIALDHLIFYLEQRTQYYAECKNLKAYRDSFAFS"
     gene            1942..2721
                     /locus_tag="VFP93_02195"
     CDS             1942..2721
                     /locus_tag="VFP93_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET9843453.1"
                     /translation="MALRIITLNVNGIRSAARKGFFSWLEKQNADIVCLQEIRAHQEQ
                     LSDPIYFPKNYHCYYVDSHKKGYSGVALFTKHQPIARVEKLGYPLADDEARYIHVEFA
                     NLIVVSLYLPSGTSGDARQQLKYAFMDWYWEYLQAQITHKKPFIICGDWNIAHKKIDL
                     KNWRSNQKRTGFLPEERAWLDKVFQEHYVDAFRVLNQDPDQYTWWSNFRNAWDNNVGW
                     RIDYQVISPLLKDKLIAASIYKEERFSDHAPLIMDYAIELV"
BASE COUNT          790 a          560 c          530 g          873 t
ORIGIN      
        1 cagcgatgac agctattcaa cttctgttat tgggcgctgt tgctttgaca gtgacagcag
       61 gactttttgc tgttgcgcga tttagtgcgc atcatgctac tatccctgtt cacccgcaca
      121 ttagagtaca tcacaatcct gcaccacagg taagggtaca tcacaatcct gcaccacagg
      181 taagggtaca ccacaatcct gcacctcatg taagagtgca tcataatcct acaccgcata
      241 ttcgaaaaca tcctgtgaat atgggtcgac ctgcgcatcc taatccaaca gttccagttc
      301 gtagcgcgta taaggctcac tttaataagc attccgctaa tttacaaaag atacccaccg
      361 tagccgcgtc tacaatatca agaccacctg tgacaactgc ctttagatcg aatatgcacc
      421 attcaagtcc tgcacatcct gtacatggat tccgtgctac aaaacatcgt gcttaagata
      481 aactaaactc tcccttggga gagtttttat tctacgctag aggaatcttg cactttgatc
      541 tcattaaaac gttcaatgag tttctcgtac tgatcgtgaa tttgtttctt ttcggtagtt
      601 tttcgaatta aaaatttcgt gttttcttca atttggaatt ggctatcttt aatagtttct
      661 tcgatttggg gtggcacggg ctgatttcta tcttggtgaa ttttcgaaat ttcttgtgct
      721 ttttgaagtt ggagctttat acgctctaaa ttatctttgg taatatcttc ctgaacttta
      781 atcgacgata atttttcatc acgcgaacgt tcgatatctt gcacattacc aaacattttt
      841 agcagcatga tatctttttt tgcttcaagc tctttcttat gtaattcttc ttctttttgt
      901 ttctcaaatt ctactttttt ttgttcttct tttaaacttt ttgcaacttc ttcatcggaa
      961 ggcttaggcg gcacaacttc tattacatta cctcttggag agatcacggt gtatccgcgg
     1021 ttagcagctt caccgggtag agacgtgctt ataatttcaa tcccgttttc atcttggtat
     1081 ttatacagat tttcactact ttgctctgca gcaaaaatct gaaaagatat agcagttcca
     1141 agaaatgcca gctggatcca ttttacgctt ttgaagaaga taagtctcat aggttaagcc
     1201 tatcacattt tgtgcagaag ttaggaaaaa gcaaaagaat cgcgataagc ttttaaattt
     1261 ttacattccg catagtactg tgttcgctgt tctaaataaa aaatcagatg atctaacgca
     1321 ataatggaac agaccgggat ttgatacatg gcaataagct cagataccgc cgtttgttta
     1381 ttttcaccgg gttcttggcg atcgaaggcg atcacaatgc cagccacacg tgcttttgtt
     1441 tgatggatta attcaatggc ttcttttttg gccaggccgg aggtaataac atcgtcaata
     1501 atgacgacat cgccgtgaag aggagcaccc attaaatgcc ccatttctcc atgcgatttt
     1561 atttctttgc gattaaaaca aacttgtttt tccaatccaa ggttcgctaa cgcaatagaa
     1621 gtagctgttg cgagtggaat acctttatag gcagggccaa aaagcagatc aaaatttatt
     1681 gcatgctgct gtagtgtttt tgcataaaag cgaccaagtt gtaaaagtgg cgtacctccg
     1741 tgaaatgcac ccgtattaaa aaagtaagga ctgtgtctac ccgattttag ggtaaaagat
     1801 ccaaagcgca gaacattgtg taaaagtgca aattctataa attcaatttg atagtccaat
     1861 aacatattct tatccttttt tcgaaaggat tttagcattg gttcgaatcc gttacactac
     1921 aaaaaaatag tgggtttcaa catggcactt aggataataa cactgaatgt aaatgggatc
     1981 cgatctgctg cgcgcaaagg ttttttttca tggttggaaa aacaaaacgc cgatattgtg
     2041 tgtttgcaag aaattcgcgc tcaccaagaa caactgagtg atcctatcta ctttccaaaa
     2101 aattatcatt gttactatgt ggattctcac aaaaaaggtt acagtggcgt ggcactcttt
     2161 actaagcatc aacccattgc acgtgtagaa aaattgggtt atcccttagc ggacgatgaa
     2221 gcacgataca ttcatgtgga atttgctaat ttaattgtcg tgtctttgta tttaccttct
     2281 ggtaccagtg gagacgcgcg ccaacaatta aaatatgctt ttatggattg gtattgggaa
     2341 tatctgcaag cacagatcac acacaaaaaa ccttttatca tttgtggtga ttggaacatt
     2401 gctcacaaaa aaatcgattt aaaaaattgg cgatccaatc aaaaacgtac gggattttta
     2461 cccgaagagc gcgcatggct cgataaagtg ttccaagaac actatgtcga tgcattccgg
     2521 gtgttgaatc aagatccgga tcaatatact tggtggtcta attttcgaaa cgcatgggat
     2581 aacaacgtcg gttggcgtat tgattaccaa gtaattagcc cattgctaaa agacaaactc
     2641 attgcagcat ctatctacaa agaggagcgt ttttccgatc acgctcctct gatcatggat
     2701 tatgcgatcg agctggttta aagttcgagt aaggttcttt gtttatcaat aag
//
LOCUS       DASTIB010000126         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_738616, whole genome shotgun sequence.
ACCESSION   DASTIB010000126 DASTIB010000000
VERSION     DASTIB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738616"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            589..981
                     /locus_tag="VFP93_02200"
     CDS             589..981
                     /locus_tag="VFP93_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9843454.1"
                     /translation="MRKRHIKKPRIDCFTRQGQVFQIPLHIANKYLVKDTEALKRSPS
                     SKPLRDADIVDAAILFAEMDKKYTEAGVLLRGTRHREQLSQKDFAEKIKITQSDLSKM
                     ENGRRPIGKIIAKRIEKEFGVDYRYFLA"
     gene            complement(1050..2621)
                     /locus_tag="VFP93_02205"
     CDS             complement(1050..2621)
                     /locus_tag="VFP93_02205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9843455.1"
                     /translation="MKKYYEIYFLKHIETPRLLLRPVQLGDEVPLNKALNNSLEVLQK
                     WQAWAKDPSMKATLDFVQRGVFAWKSGFVIDFPMVIILKTEQKIIGVSGYNENCKLND
                     GLYEIGYWCDVDYQGQGFITECANALTRYAFHALNATKVILSMQVENKKSEAVATRLH
                     FNNRGIKDRDPADCVSERPEFNYVYAINNLQNLPPLELTWQHTKEDNIFSQIEWAKKT
                     LSTDSKAFASSKFIVKTLWSNIIEFNTGNAFFYLKHTPELIALDAPVIKILREEFNIS
                     VPKVIAHNKDLHCFLMHDAGRNLRGILKQEFNEALLYKAIDTFTSMQINVADHINVFL
                     HIGVPDWRLDKLPSLYAQLLSQKTLLLADGLPEFEMGEHEALLPKVSYLCEKLSAFSI
                     KQTIVQPDFNDNNMLVDDLQNITLIDLGEIAISHPFFSLLNCLQQMQKHYGFTETDAI
                     YLKIKEACLKNYLNFESHKNILEALDIAKELWPVYGAVAMYRFMQACGEDNILSFQPG
                     ALVSSLKEFMSMQGD"
BASE COUNT          984 a          472 c          524 g          922 t
ORIGIN      
        1 tttatgctat tgcattagca gatgaaaaag taagtgacaa agcaaaggaa tggctaaaaa
       61 ttctacgaga gttaatagca actaatcagc atcttttgta aagaaggctt ttaggttttt
      121 ttttaaaacg atttaaagtc atgtcaaaaa tccaaagatg taagctatat tacttacatc
      181 tttgggtaat atgcggtgaa cattacgttt aaatgcgaat agatttagtg tgattgcact
      241 aaatgtaatt taacctataa atttctgatg gtgtatggta aaatttgcca tatggcaata
      301 tttaccatat aataatattt atgacttgga aagtcaattt tactaagaaa acggctaagc
      361 agattggtaa attatcacaa aaaactcagt ttactctaag attattagta aaggatctag
      421 agactatagg accaaatgcg cagaagtggc ctaattatag taagttgaag ggtttaaatg
      481 gcgataaaag gcattgccac ttaataaaag gaaagccaac gtatgtatgc tgttgggaag
      541 tgatagataa acaaattaaa ttaattgagg tttactatgt cggaacccat gagaaagcgc
      601 catattaaaa aaccacgtat tgattgtttt acaagacaag gtcaggtttt tcaaatacct
      661 ttacacatag ccaataaata tttggtaaaa gataccgagg ctttgaaaag atcgccctca
      721 tcgaaacctt tacgcgatgc tgatattgtt gatgctgcta ttttgtttgc agaaatggat
      781 aaaaagtata cagaagcagg tgtattactt cgtggaacac gccatcgtga acagctatca
      841 caaaaagatt ttgcagaaaa aattaaaata acgcaaagtg atttatcaaa aatggaaaat
      901 ggtagacgtc ctatcggtaa gattattgca aaaagaatcg aaaaagaatt tggcgttgac
      961 tatcgttatt ttctcgcgta aaaatatctt aatcttcagt tccacaaaat aatcgtaaca
     1021 cttgccaaat gggtgttatg ctaatagtct taatcaccct gcatactcat aaattctttc
     1081 agtgaactaa cgagtgctcc aggctgaaat gaaagtatgt tatcttcacc acaagcttgc
     1141 ataaatctat acattgccac ggcgccatac actggccata attcttttgc aatatctaaa
     1201 gcttctagta tatttttgtg cgattcaaaa ttgagatagt tttttaaaca agcttcttta
     1261 atcttaagat aaattgcatc ggtttctgtg aaaccataat gtttttgcat ttgttgcaag
     1321 caattcagca gcgaaaaaaa tggatgtgaa attgcgattt cgcctaaatc aatgagggta
     1381 atattttgta gatcatctac aagcatatta ttatcgttaa aatcaggctg aacaatggtt
     1441 tgtttaatgg aaaaggcaga caatttttca cataaataag agactttggg tagcagcgcc
     1501 tcatgttcac ccatttcaaa ttctggtaac ccatcggcga gcaaaagggt cttttgtgaa
     1561 agcaattgcg cgtataaaga aggcaactta tctaaacgcc agtcgggcac accgatatgg
     1621 agaaaaacat ttatgtgatc agcaacgttt atttgcatgg aggtaaaagt atcaatcgct
     1681 ttataaagca gagcttcatt aaattcctgc tttagtatgc cacgcaaatt tctgccggca
     1741 tcatgcatta aaaaacaatg taagtcttta ttatgggcaa tcactttggg tactgaaata
     1801 ttaaattctt cccgaagaat ttttataacc ggcgcatcta aagcaattag ttctggtgtg
     1861 tgtttaagat agaaaaatgc attaccggta ttaaattcaa taatatttga ccacagcgtt
     1921 ttgacaataa acttgctact cgcaaaggct ttgctgtctg tagagagtgt tttttttgcc
     1981 cattcgattt gagagaatat attatcttct ttcgtgtgct gccaagttaa ctctaaggga
     2041 gggagattct gaagattatt aatagcataa acataattga actcaggtct ttcggaaaca
     2101 caatctgcag gatctcgatc cttaatacct ctattgttga aatgtaatct tgtagcaaca
     2161 gcttcgcttt ttttattttc tacctgcatc gataggataa ctttggttgc attgagggcg
     2221 tgaaatgcgt atcgagttaa agcattagca cattcggtga tgaatccttg tccttgataa
     2281 tctacatcgc accaataacc tatttcatat aaaccatcgt taagcttgca attctcgttg
     2341 tatccactga caccaataat tttttgttct gtctttaaga ttattaccat cgggaaatct
     2401 atgacaaaac ctgatttcca ggcaaaaaca ccacgttgaa caaaatcgag cgttgccttc
     2461 atactgggat ctttggccca tgcttgccat ttttgtaaga cttctaaaga attatttagg
     2521 gctttattga gcggaacttc atctcctagt tgtactggtc ttaataacaa gcgaggcgtt
     2581 tctatgtgtt ttaggaaata aatttcataa tattttttca taagaatctg ctttgcctgt
     2641 acgaagttct gtgatttaaa taggctcagt cactcgaatc ataatccttt tgtctcttac
     2701 aggaaactac aaaaaatctt aaacacatag acagaagaat agttagcgat tgaaattaaa
     2761 ataatctctg aaatattaat atgtatttaa aaaaataatg atgtatgaca ttcataggaa
     2821 gataatgctc atacttatcg aaacgaaaag gctgatttta agcttggaaa aaagaagatg
     2881 cagaggccta tttttaaatt aa
//
LOCUS       DASTIB010000127         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1061641, whole genome shotgun sequence.
ACCESSION   DASTIB010000127 DASTIB010000000
VERSION     DASTIB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061641"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFP93_02210"
     CDS             <1..277
                     /locus_tag="VFP93_02210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9843456.1"
                     /translation="ESGTRFKILEAGACKVPMVSTTLGAEGLDVTHLQDILIADKPDE
                     FSNHIVKLVLDKSFAASLAQASYELVKKKYSLNMLKSQANRILEKLT"
     gene            510..1340
                     /locus_tag="VFP93_02215"
     CDS             510..1340
                     /locus_tag="VFP93_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9843457.1"
                     /translation="MSFSKRIYDIEAGKVDKNYWRDLWRYRGLFYFLAWRDILVRYKQ
                     TLIGVTWALIQPLLMMIVFSVVFGKLANFPSYGVPYPILVFAGMLPWQFFALSFAESS
                     NSLVTNSNMISKVYFPRLILPSSTIIVCLMDFFIASVLLIVLMLWYGFLPSRTIIFFP
                     IFLLLACMTSLGAGLWVAALNVRYRDFRYVIPFVIQLGMYISPVGFVSNIIPEKWRMI
                     YSINPLVGVIDGFRWSLLGDTFLVYWPGLVCSIVVITTLLISGFIFFRKTEKTFADII
                     "
     gene            1340..2638
                     /locus_tag="VFP93_02220"
     CDS             1340..2638
                     /locus_tag="VFP93_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HET9843458.1"
                     /translation="MTSIIEVENLSKKYILGNRQNEPYATLRDKIAYQAMHFGKQIVS
                     HAYRRQVQSTKNEIFWALDQVTFNVHEGERIGVIGSNGAGKSTLLKLLSRITEPSAGR
                     IRVKGRVASLLEVGTGFHPELSGRENIYLNGAVLGMSKVEIKNKFNEIVEFAEVSKFL
                     DTPVKRYSSGMYVRLAFAVAAHLEPEILLVDEVLAVGDASFQRKCLGKMKTVSESGRT
                     IIFVSHNLQAISALCQRAIYLEHGQLRAIGPTQEIINQYLETVKRKVSQGNAHIVLPK
                     KQNQSAHITEFSVENNSGKSTLRFDITESILIRFNFHLDIAYQTLLIVLHVWDKDNNL
                     VLASLDSDWSNYHNQTMHDNFPREKGDYQGLVTIPAPMLNQGIYELEFILTLGPSRID
                     TIRGIYIEIDDNGSFLSYVTKSSRGGLLATPLKWQIDEKD"
BASE COUNT          835 a          432 c          548 g          895 t
ORIGIN      
        1 tgaatcaggt acccgcttta agatattaga agctggagca tgcaaagtgc caatggtttc
       61 aacaacacta ggagcagaag gtctagatgt cacgcattta caagacatat tgattgcaga
      121 taagccagat gaattttcta atcatatcgt aaagcttgta ctagataagt cttttgcggc
      181 atcgttggcg caagcaagtt atgagttggt taagaaaaaa tacagcctta atatgctaaa
      241 aagtcaagca aatcgaattt tggaaaaact tacataatat taaagcttta catttatatt
      301 cgaaaatttt atgccttagt ttattttgca tttaaaaact catgatcttg gagttgatga
      361 aatgtttgaa atcaagcaaa cctttttatg atatagcctg catcgtaaat tagattgttt
      421 ttagttgagc ataaaagtaa aattgcctct tatgctccgt gccataaata gaattctcat
      481 ttatccatcc ttaacgaagg gcatcgcata tgtcattctc aaaaagaatt tatgacattg
      541 aagcaggcaa agtagataaa aattattggc gagatttgtg gcgatatcga gggttatttt
      601 actttctagc ttggcgcgat attctagtcc gttataaaca gacacttata ggtgttacgt
      661 gggccctcat acaaccactt ctgatgatga ttgtcttttc tgttgtcttc ggtaaactag
      721 ccaacttccc atcgtatggc gtgccttatc ccatcttagt ttttgcgggt atgttaccgt
      781 ggcagttttt tgctttatca tttgctgagt caagcaatag tttggtgact aattctaaca
      841 tgatttcaaa agtttatttt cctaggctta tattaccttc aagcacaatt atagtctgcc
      901 ttatggattt ttttattgcc tcggtgcttt taatcgtatt gatgttgtgg tatgggtttt
      961 tgccttctcg cacaattata tttttcccta tctttttgtt attggcttgt atgacatcgc
     1021 ttggggcagg attatgggtt gctgcattaa atgttcgata tcgtgatttt agatacgtga
     1081 tcccttttgt tattcaactt ggaatgtata tttcgccggt tggttttgtt agcaatatta
     1141 ttccagagaa atggcgcatg atttattcaa taaatccttt agttggtgtt atcgatgggt
     1201 ttagatggtc gttacttggg gatactttcc tggtgtattg gcctggttta gtttgttcaa
     1261 ttgtagtcat aaccacgcta ttaatttcag gttttatatt ctttcgaaaa acagaaaaaa
     1321 cctttgcgga tatcatttaa tgacaagcat tattgaagtt gaaaatttaa gtaaaaaata
     1381 tattttagga aatcgacaga atgaacctta tgctactttg cgcgataaaa tagcctatca
     1441 agcgatgcat tttggtaagc aaatagtgag ccacgcctat aggaggcaag tccaatcaac
     1501 gaaaaatgaa attttttggg ctttggatca ggtaactttt aatgtccatg aaggtgaaag
     1561 aattggtgtc attggttcta atggagcagg taaatctaca ttattgaagt tactgagtcg
     1621 cattacagag ccttcggcag gtcggattag agtgaaaggc cgtgtagcaa gtttattaga
     1681 agtaggcacg ggttttcatc cggaattgag tggaagggaa aatatttatt taaatggcgc
     1741 cgttcttggc atgtctaaag tggagattaa gaataagttt aatgagattg ttgagtttgc
     1801 tgaagtatca aagttccttg ataccccggt aaaacgttat tcttcaggca tgtacgtcag
     1861 actagctttt gcagtagcag cacatttaga acctgaaata ctcttagtag atgaagtgct
     1921 agcagtaggt gacgcttctt ttcaacgtaa atgccttggc aagatgaaaa ctgtaagtga
     1981 aagcggccgt actataatat ttgtgagtca caatttacaa gcaataagtg cgttgtgtca
     2041 gcgtgcaatt tatttagaac atggacaact tagagcaata gggccaacac aagaaataat
     2101 caatcaatat ctagagactg ttaaacgcaa ggtatcacaa gggaatgcac atattgtctt
     2161 gcctaagaaa cagaaccaat cagctcacat tactgagttt tctgttgaaa acaattctgg
     2221 aaaatccaca ctgcgatttg atattacaga aagtatatta attcgattta atttccattt
     2281 ggatattgct tatcaaacgc tgttaatagt cttgcacgtt tgggacaaag acaacaattt
     2341 agtgttggca tctttagatt cagattggtc aaattaccat aaccaaacca tgcatgataa
     2401 ttttccaaga gaaaaaggag attatcaagg tttagtcacg ataccagcgc ccatgctcaa
     2461 tcagggtatt tatgaattag agtttattct tacgttagga cctagtcgta tagatacaat
     2521 aaggggtatt tatatcgaaa ttgatgataa tggcagcttt ttatcctatg tgacaaagtc
     2581 ttcgcgagga gggctgcttg caacaccact taaatggcaa atagatgaaa aagattaaac
     2641 acaggattcg ataaagaggc aacatgtata gtattaatat aactcagcag aaaaattctt
     2701 tgaaatcttt
//
LOCUS       DASTIB010000128         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1446655, whole genome shotgun sequence.
ACCESSION   DASTIB010000128 DASTIB010000000
VERSION     DASTIB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446655"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1769
                     /locus_tag="VFP93_02225"
     CDS             129..1769
                     /locus_tag="VFP93_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9843459.1"
                     /translation="MTMEKGHLLENPTRLSNSSLWQLQKNFFAHRGVEAWNQEIPFFI
                     SSNAFTGTRYAQLIVQYILDLFDPNEMATFYIVEVGAGHGKFSFYCIKALEEAINNLH
                     IQGLQYQYIVTDVAQKNIDFCQNNEALKPLIENNRLDFAVFDMETDEDFTLVHQKVLF
                     SSLDKIYPVLFICNYVFDVIRQDAFLIKDNALFEIAVGLHSRYPKFDPQRPIIRELKL
                     NYAYQKIEAKDYYSEPVLKDLLSQYSELFQQKEAVILIPVGAFQFVSKMQKLCEKFLL
                     ICGDKGLATAENLADIDVSTMYTFDGCFAFSHNFHALGEYFKAQNGDYLPTQHHNDFK
                     ICLYAHNHDFTSLPKTTHFFKSILEAMGPDEFVYQNEEFAYNNYRYSLKACLSFLRSS
                     YFEPDTYFHMHDKIMEQLPILSENFLYDVKQALRKVEENLYHYQSFYDVFPLLGFFYY
                     QTKAYKKAEFLYKKALKYSKDPFNALRGLGLLYEARGNTNRALEYYNKALSVRSDSII
                     KERIRTLEGNPLIMLKPLLKMAFVFGLLAGLLYLTLFR"
     gene            complement(1807..2046)
                     /locus_tag="VFP93_02230"
     CDS             complement(1807..2046)
                     /locus_tag="VFP93_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843460.1"
                     /translation="MRNLAVNEMNAVAGGTDVYYFDLYAGVEVVGMKATIIGYKPTAS
                     WIEPGFLVDHHYEIQEPIYVFEPIYSETVTTVTYY"
     gene            2395..>2832
                     /locus_tag="VFP93_02235"
     CDS             2395..>2832
                     /locus_tag="VFP93_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HET9843461.1"
                     /translation="MKNKNKKDTNILAIHPRQGGRREGAGRPKGTGKYGEPTMAIRVP
                     ESWGIKIQSLLQVKQKDKHLASALVVSDIWQPRFQSETALPLFTHKIAAGFPSPAEDH
                     IEASLDLNEYLIEHPAATFYVRVEGESMVNAGIYPNDILIVDRA"
BASE COUNT          886 a          497 c          528 g          921 t
ORIGIN      
        1 tggacgcaag tcaatcccaa aattcctagt aatatgttgc gggcgttaca acaagtaaac
       61 cccagtcatt tgatggataa aaatttatgg gattttacgc aattagaaaa agaaatgaag
      121 ggactgctat gaccatggaa aaaggacatc tgcttgaaaa tcccacccga ctttcaaact
      181 cttcgctgtg gcagcttcaa aaaaactttt ttgcacatcg tggggtggag gcttggaatc
      241 aagaaattcc tttttttatt tctagtaatg cttttaccgg aacgcgttat gcccaactta
      301 tagtgcaata tattcttgat ctatttgatc ccaacgaaat ggcgactttt tatattgtgg
      361 aggtcggcgc aggccatggt aagtttagct tttattgcat taaggcgtta gaagaagcaa
      421 taaacaattt acacattcag ggtttacagt atcaatacat tgttaccgat gttgcccaaa
      481 aaaatatcga tttttgccaa aacaatgaag ctttaaagcc actgatagaa aataatcggc
      541 tggattttgc tgtatttgat atggaaacgg atgaagattt tactttagtc caccaaaagg
      601 tactgttttc ctctttggat aaaatctatc cggtgttatt tatttgtaat tatgtgtttg
      661 atgtaatacg ccaagacgct tttttgataa aagacaatgc tttatttgag attgctgtgg
      721 gtttgcatag tcgttaccca aaatttgacc cgcaacgtcc tatcattcga gaacttaaat
      781 taaattatgc ttaccaaaaa attgaagcga aagattatta ttcagaaccg gtattaaaag
      841 atttgttgag ccagtatagt gaattatttc aacaaaaaga agcggttatt ttaatcccgg
      901 tgggggcgtt tcaatttgtg agcaagatgc aaaaactgtg tgaaaaattt ttattaattt
      961 gtggggataa aggattagca actgcggaaa atctagcaga tattgatgtt tcgaccatgt
     1021 atacctttga tggttgtttt gccttttcgc ataattttca tgcgttagga gaatatttta
     1081 aagcgcaaaa cggcgattat ttgccgacgc aacatcataa cgattttaaa atctgtttgt
     1141 atgctcataa ccatgatttt acatctttgc caaaaaccac ccattttttt aaaagcatat
     1201 tagaagcgat gggtcctgac gagtttgtat accaaaatga agaatttgca tataacaatt
     1261 atcggtattc cttaaaagcc tgcttatcct ttttacgttc cagctacttt gagccagaca
     1321 cgtattttca tatgcacgac aaaattatgg aacaattacc cattttgtcg gaaaattttt
     1381 tatatgatgt aaaacaagcg ctacgtaaag ttgaagaaaa cttataccat tatcagagct
     1441 tttatgatgt ttttccgtta ttaggatttt tttattatca aaccaaagcc tacaaaaaag
     1501 cagagttttt atataaaaaa gcgctaaaat atagcaaaga tccgtttaat gccttacgag
     1561 gattaggtct tttgtatgaa gccagaggta atacgaaccg agcgttggaa tactataata
     1621 aggcactttc ggtacgatca gattccatta taaaggagcg tattcgaacg ctagagggaa
     1681 atcctttaat catgctcaaa cctttgctga aaatggcctt tgtatttgga ctattagcag
     1741 gtttgcttta tttgactttg tttagataaa ttcataaaaa ggcagcttaa gctgcctttt
     1801 ttattcttaa taataggtaa ccgttgttac tgtttctgag taaataggtt cgaacacgta
     1861 aataggttct tgaatttcat aatgatgatc taccaaaaag ccgggttcaa tccagcttgc
     1921 agtgggttta tagcctatga tggttgcttt cattcctaca acttcaacac ctgcatataa
     1981 gtcaaaataa taaacgtctg ttccacccgc taccgcattc atttcattta ctgctaaatt
     2041 acgcatgttt ttctccgact ataaatttaa attctcgggg cgttaagatt gctctcaacg
     2101 gaacttatca taccgaaacc aagaattttt aaaaccccat ctaattcctt ttatccaatc
     2161 ttgtatgccg tttatctgtt ttatagcacc gcatatctgt gttaactaac ttttttaatt
     2221 gggattttcg ctttatagaa tgctaaaggg taggtaatcg aataaacctt taatcaaaag
     2281 cgatttggtt tattaacggg tcctaattag aattttttta caaaaaccca ttttacctct
     2341 tgtttttttg gcggaccctg ccataattcc atgaaaagtg tacttgaatc atttatgaaa
     2401 aataaaaata aaaaagatac aaatattcta gctatacatc ctcgtcaagg cgggcgtcgt
     2461 gaaggagcgg gtcgccctaa aggtacaggt aaatatggtg agcctactat ggctattcgg
     2521 gtcccagaaa gttggggcat taaaattcaa tcgttgttac aagtgaaaca aaaagacaaa
     2581 catctcgcat ccgctttagt ggttagcgat atatggcagc caaggtttca aagcgaaacg
     2641 gctttacctt tatttaccca taagatcgca gcaggattcc cttctccggc tgaagatcat
     2701 attgaagctt ccttagattt aaatgaatat ctaatcgagc atcccgccgc aactttttat
     2761 gtgcgcgtgg agggggagtc tatggtaaat gctgggattt atcctaacga cattttaatt
     2821 gtagataggg ca
//
LOCUS       DASTIB010000129         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_266838, whole genome shotgun sequence.
ACCESSION   DASTIB010000129 DASTIB010000000
VERSION     DASTIB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266838"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1236)
                     /gene="ychF"
                     /locus_tag="VFP93_02240"
     CDS             complement(145..1236)
                     /gene="ychF"
                     /locus_tag="VFP93_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET9843462.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIDAANYPFCTIEPNVGIV
                     PVPDPRLDMLANIVKPEKIVPTTMEFVDIAGLVKGASQGEGLGNKFLANIRETDAIAH
                     VVRCFDDVDVVHVSGKVDPKNDIEVIHTELLLSDLEAVEKALVKIQKVAKSGNKEAIL
                     EKEMLEALRDHLDAGKPGRTFPQNEDTKLLFEKVHLLTAKPIMFVANVEENGFDNNPL
                     LAQVEEIAKQEKGIVVPVCAALESEIAELAEEDKKEFLESLGLIEPGLNRVIHSGYQL
                     LNLHTYFTAGVKEVRAWTIPLQCKAPQAAGKIHSDFEKGFIRAEVVSYEDFVKYQGEV
                     GAKDAGKWRLEGKDYIVHDGDVIHFRFNV"
     gene            complement(1246..1821)
                     /gene="pth"
                     /locus_tag="VFP93_02245"
     CDS             complement(1246..1821)
                     /gene="pth"
                     /locus_tag="VFP93_02245"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9843463.1"
                     /translation="MGIKLIVGLGNPGQQYQETRHNAGVWFLQKLTSYHNIALTTESK
                     FKALLGRADLHSHDMRFCVPLTFMNLSGDSVATIAHFYKIVPEEILVAHDELDLPVGT
                     IRLKFDGGHGGNNGVRDVIAKLQTKRFYRLRIGIGKPSHKDDGANYVLSKPSRADRNT
                     IDTCIDKAIDIMPEFIRGNIDKAMQALHTNS"
     gene            complement(1824..>2197)
                     /locus_tag="VFP93_02250"
     CDS             complement(1824..>2197)
                     /locus_tag="VFP93_02250"
                     /inference="COORDINATES: protein motif:HMM:NF026044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9843464.1"
                     /translation="FQRVSATQNLVRFVPVHFINEDIAPGVKAGGIVSHHVTEVEIRC
                     LPKNLPEFIAFDLSNAQINEVYHLSDLTLPEGVELVDISAGHEDNKALVSIHLPRGSV
                     AEETPEQPTPTPEAGKADKKA"
BASE COUNT          608 a          487 c          414 g          688 t
ORIGIN      
        1 aaagctaagt tatcaaagcg aagaatcttt atgtaaattg atcaaggatt tacaaaataa
       61 aagattacct atgtatagcg ccttaatcct ggaagtagat aacgcgctaa cacaaaaggt
      121 tagttcgagt tgtaagttac atgtttaaac attaaaccta aaatggatta cgtcgccatc
      181 gtgtacgatg taatccttac cttctaagcg ccattttcca gcatctttcg cacccacttc
      241 gccttggtat ttaacaaaat cttcatagct caccacctcg gcacggataa aacctttttc
      301 gaaatcgcta tgtattttac cggccgcttg cggagcttta cattgtaatg gaatggtcca
      361 cgcgcgcact tccttaacac ctgcggtaaa ataggtatgc aaattaagta attggtagcc
      421 gctatggatt acacgattta agcccggttc tattaggcct aagctttcta aaaattcttt
      481 tttatcttct tcggcgagtt ccgcaatctc agactctaaa gccgcacaga caggaactac
      541 gatccctttt tcttgttttg caatttcttc gacttgggcc agcagcgggt tgttatcaaa
      601 cccattttct tcgacatttg ccacaaacat gattggtttt gctgttaata aatgcacttt
      661 ttcaaaaagc agtttcgtat cttcattttg tgggaaggtt ctgccaggtt ttcctgcatc
      721 taaatggtct ctaagtgctt ctagcatttc tttttctaag atggcttctt tgttaccact
      781 ttttgctact ttttgaattt ttactaaggc tttttctacg gcttctaagt cgcttaataa
      841 aagctcggta tggatcactt caatgtcgtt ttttggatcc actttgcccg atacatgcac
      901 tacgtctaca tcgtcaaaac aacgtaccac gtgcgcgata gcatcggttt cacgaatatt
      961 ggctagaaat ttgttgccta atccttcccc ttgggaggcg cctttaacaa ggcccgcaat
     1021 atccacaaat tccatggttg tcggtacaat tttttccggt tttacaatat ttgccaacat
     1081 atctaagcga ggatcaggaa ccggaacaat acctacattg ggctcaatcg tacaaaaagg
     1141 gtagttggca gcatcaatac ctgctttcgt taaagcatta aaaagcgttg atttacctac
     1201 attaggaagg ccaacaatac cacatttaaa acccatgttt acctcttaag aatttgtatg
     1261 cagcgcttgc atcgctttat cgatattgcc acgaataaac tccggcataa tatcgattgc
     1321 tttgtcgata cacgtatcaa tggtatttct atctgctcgg gaaggtttac ttaatacgta
     1381 atttgcacca tcgtctttat gtgacggttt gccgatgcca atgcgtaatc tataaaagcg
     1441 ttttgtttgt aattttgcaa taacatcccg cacaccatta ttgccaccat gaccgccatc
     1501 gaattttaat cgaatagtac caaccggcag atcaagttca tcatgggcaa ccaagatttc
     1561 ttcgggaacg attttataaa agtgcgcaat agtcgctacc gaatcgccac ttaaattcat
     1621 aaatgttaaa ggcacgcaaa aacgcatatc gtgactgtgc aaatcggccc tacctagcaa
     1681 tgctttaaat tttgactcag tggttagagc gatattatgg tacgaagtta gtttttgtaa
     1741 gaaccaaacg cctgcgttgt gtcgtgtttc ttgatattgt tggccgggat tccccaagcc
     1801 aacaatcaat ttgatgccca tccttaagct ttcttgtcag cctttcctgc ttcaggagtc
     1861 ggagtaggct gttcaggagt ttcttctgca acactaccgc gcggtaaatg aatgctcacc
     1921 agcgctttat tgtcttcatg acccgcagaa atatctacca attcaactcc ctcaggcaga
     1981 gttaagtctg aaaggtgata aacttcgttt atttgcgcgt tgcttaaatc aaacgcaatg
     2041 aactcaggaa gatttttagg taggcagcga atttccactt cggttacgtg gtgagaaact
     2101 ataccgccag ctttaacacc aggagcaata tcttcattta tgaagtgaac tggcacaaaa
     2161 cgtactaggt tttgggtagc acttacacgt tgaaaat
//
LOCUS       DASTIB010000130         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_242060, whole genome shotgun sequence.
ACCESSION   DASTIB010000130 DASTIB010000000
VERSION     DASTIB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242060"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFP93_02255"
     CDS             <1..235
                     /locus_tag="VFP93_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843465.1"
                     /translation="AEKHNSYMDLIQFCVKDPINCFASVLNTYLTRRIQELEQVQDVN
                     MENANFFTPSYDDSKKRDHDDEPDTSVKFRRLV"
     gene            complement(634..>2431)
                     /gene="parC"
                     /locus_tag="VFP93_02260"
     CDS             complement(634..>2431)
                     /gene="parC"
                     /locus_tag="VFP93_02260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET9843466.1"
                     /translation="FDGTLEEPSILPARLPTLLLNGTTGIAVGMATDIPPHNLTEVTN
                     ACIHLLDNPDTTLDDICKLIPAPDFPTTAEIITPKKELKQIYKVGYGSLKMRAIYLIE
                     DGDIVITALPHQVSGAKVLEQIAQQMQSKKMPFIADLRDESDHENPTRLVIIPKSNRV
                     DKEMLMSHLYATTDLERSYRVNMNVIGLDGRPQVKPLLTLLTEWLEFRTQTVKRRLQF
                     RLEKVLARLHILDGLLIAFLNIDEVIEIIRHEDNPKAVLMKRFNLSEIQTDAILDLKL
                     RHLAKLEEVKINGEQKDLNSERDKIEKTLASAKKLKTLIRKELEEDCKAFGDKRRSPL
                     VTREEAKPIKETERIPSDPVTVILSKMGWIRSAKGHEIQGQELSYKASDEFLAQAQGK
                     SNDQIVFLDSTGRSYALLAHSLPSARGFGEPLSATLKVPSGAHFVSLLMTEVSQKIML
                     ASSAGYGFITNFGELLTKNKTGKAIIKCPHNSVPLPIIKVEESHRMVALTTNEGKLLL
                     VDIKDIPELPKGKGNKLISIASERVAKKEAFVVGVALLGPKDSLLIIAGKRKVTLSPH
                     DLDNYFGERGRKGNNLPRGVQKVTALEVIKKA"
BASE COUNT          649 a          491 c          472 g          819 t
ORIGIN      
        1 tgcagaaaaa cataatagct atatggatct tattcaattt tgtgtaaaag atcccatcaa
       61 ttgctttgct tctgttttga atacatattt aacaaggcgg attcaagaat tagagcaagt
      121 tcaagatgtg aacatggaaa atgctaactt tttcacgcct tcttacgatg attctaagaa
      181 aagagatcat gatgacgagc cggatactag cgtgaagttt agacgtcttg tgtaaattct
      241 attgcttttt ctgcattccc cgcgagcgag aagcgaatag cgggatccag gattaatcag
      301 cttcgtaagc gataatctat gatggatccc gtgtcgtcgg gcgcgaaagt tttgtatttt
      361 ttactatgat tgtgagcgcc ctttgcacgg gacgacggat aagatccaga tcgaccagct
      421 tcgttagcga taatctatga tggatcccgt gtcgtcggtg cgcgaaagtt ttgtgatttt
      481 tactactatt gtgagcgctt tgcacgggac gaggataaga tccaggattg atcagcttcg
      541 ttagcaataa tccatgatgg atcccgtatc gtcggcgcga aagtgttgtg ttttttacta
      601 ctattgtgag cgccctttgc acgggcgaca ttcctaagct ttttttatta cttcaagcgc
      661 tgtgactttt tgcacgccac gcggtaaatt attacccttt cttcctcttt caccaaaata
      721 attatccaga tcatgaggag aaagcgtaac ttttcgttta ccagcaatta ttaataaact
      781 gtcctttggt cctaatagtg caacacctac cacaaaggct tctttttttg caacacgttc
      841 ggaagctatg gatattaatt tattgccttt gcccttgggc aattctggta tatctttgat
      901 atctactaac aataacttcc cttcattggt agtaagcgcc accatcctgt gcgactcttc
      961 cacttttata ataggtaaag gcaccgaatt gtgtgggcac tttataatcg ctttaccggt
     1021 tttatttttt gttaataact cgccaaaatt tgtaataaat ccataacctg cactcgaagc
     1081 caacataatt ttttgcgaca cttcggtcat aagtaaggat acaaaatgtg cgcctgatgg
     1141 tacctttaag gttgcgctta atggttctcc aaatccgcga gctgaaggga gtgaatgcgc
     1201 taatagcgca taagagcgac cagttgagtc taaaaataca atttgatcgt tcgatttgcc
     1261 ttgtgcctga gctaggaatt catcacttgc tttgtagcta agttcctgtc cttgtatctc
     1321 atgaccctta gcggatcgaa tccatcccat ttttgataaa ataacggtga ctggatccga
     1381 tggaatacgc tcagtttctt ttatcggttt tgcttcctct cttgttacta aaggcgatcg
     1441 tcttttatcg ccaaaagctt tacaatcttc ttccaattcc tttcgaatta gggtttttaa
     1501 ttttttagcg gaagctaaag ttttttctat tttgtcgcgt tcgctattaa gatctttttg
     1561 ttcgccatta atttttactt cttccagttt ggctaagtgg cgtaatttta aatctagaat
     1621 agcatcggtt tgaatttctg ataagttaaa acgcttcatt aaaactgctt tcggattatc
     1681 ttcatgacga ataatttcta tcacttcatc gatatttaaa aaagcaatta acaagccatc
     1741 taatatatgt aatctggcca atactttttc taaacgaaac tgtaatcgac gctttaccgt
     1801 ttgcgttcta aactctaacc attcggttaa taaagtaaga aggggtttta cttgtgggcg
     1861 accatccagg ccaataacat tcatgttgac ccgatagcta cgctctaaat cggttgttgc
     1921 ataaagatgc gacattaaca tctctttatc gacacgattt gattttggaa taattacgag
     1981 acgcgttggg ttttcatgat ccgattcatc gcgcaaatcc gcaataaatg gcattttttt
     2041 cgattgcatt tgctgcgcta tttgctctaa aactttagca ccacttactt gatgcggtag
     2101 agctgtaatc acaatatcgc catcttctat gaggtaaata gcgcgcatct tcaaactgcc
     2161 ataccctact ttgtaaattt gtttcagctc ttttttaggg gttattattt cagcagtcgt
     2221 tggaaaatcg ggcgctggta ttagtttaca aatatcatct aatgttgtat ctggattatc
     2281 taacaaatga atacaagcat tagtcacttc cgttaaattg tgggggggaa tgtcggttgc
     2341 catacccacg gcaattcctg tcgtgccatt taatagcagt gtcggtaacc ttgccggtaa
     2401 tatactaggc tcttctaagg ttccatcaaa a
//
LOCUS       DASTIB010000131         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_316951, whole genome shotgun sequence.
ACCESSION   DASTIB010000131 DASTIB010000000
VERSION     DASTIB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316951"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFP93_02265"
     CDS             <1..565
                     /locus_tag="VFP93_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET9843467.1"
                     /translation="TGIFLWACVLKSGVHATCAGVILGLFIPIKIQNNKKISPLKKLE
                     NNLYPWVSYGILPIFAFANAGVSFEGLTFTMLQSPITLGIASGLFFGKQIGVMSMSAL
                     AIILKLANLPPKVNWLQFYGLALITGIGFTMSLFIGTLAFYDLETQAAVRLGVLIGSL
                     FSAILGCIIIVIANKRSIVPSIQTGTS"
     gene            complement(602..3331)
                     /locus_tag="VFP93_02270"
     CDS             complement(602..3331)
                     /locus_tag="VFP93_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843468.1"
                     /translation="MQHPNQANSTLNINNTDKKGNTLLHRAIEHGSNHGVRKLIREGA
                     NIEAKNNKRQTPLHLAILHHKPRFATIWSALLQSLYLKNDRADIVKFLVNEGNANVNA
                     VDSQGDTPLILATKLAKPELVYFLLNDGKSDPRIKNRFEQNALHVAAEKGRSNIAKML
                     IDSNFDLLNTSDLWGHTPLHLAISESQFEIVKLFLESDHACVNTADNKNKLPLHYCAN
                     QTNSAITKLVLTKSKTAINKLSNEGFTPLHLAVLNNRLANAKLFIQDGGADVNILFPN
                     GESLLHYALRNHQNTLTKLLINNNADVNEKNLDGNTPLHCALLELNFEMVEHLISEAA
                     LVNLQNNEGITPLHYAAKLGYDSAVDTLLKSSNIDPNLKDKRGENALFYAVKSHNIHA
                     VTALLKNANLNLNSVNNHGETIMHIAIRFYHKEIFELLMNQNTIDFTMSDLHGVNPLA
                     LAASIRKSACIKSLLTMSAVRNAPNTPQILLSNNVLLLACQVGDFELVNRLLEFPNIL
                     DNVTMNNNEAFRIARANNYRQIIERLISIAAVRDFANAEDLQQINLHQIAADNENATI
                     ALDNQELGLLVNIKKYYGEKFRTLGQNTILANYQDFLKSQYDHTPAFDAKGNKLPLEP
                     YNGAMEQKPYYKNIWHTGWRYFLRPNPWILKDMPFADILPNGRAAAIADEDKENLAYL
                     WLAVSDDTIILENNISNQDLKECFTREIALIARAHNWDAKRRNHHGLLEEYDDEKEDK
                     PTCAIGVSRRLIQSAFQHPLAQKPESRELTPSIFRRKFLEQLLSENPAIKNNLFYQFN
                     NFSLEQLRGLKNALDLEIEECIPLSDEQNALFQISDDAVALFIEDAKFWFGSARITEQ
                     PLMWGQSYKNYEQFAVDLAKQCKKYCYSEIYDILNGIIKDRLNQPSTRAKLAL"
     gene            complement(3503..>3736)
                     /locus_tag="VFP93_02275"
     CDS             complement(3503..>3736)
                     /locus_tag="VFP93_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843469.1"
                     /translation="EQRRQEQLRAQQLQRMADLKRQAEQNKLKKKFQAQQQPKGQVRV
                     IRFLGNAQPIGFAKLQKRAVLQNSHIAAKCVLF"
BASE COUNT         1077 a          643 c          674 g         1342 t
ORIGIN      
        1 tacgggtatt tttttatggg catgcgtttt gaaatcaggt gtgcatgcta cgtgtgcagg
       61 cgtgatcctt ggacttttta ttccgatcaa aattcagaat aataagaaaa tatctccttt
      121 gaaaaaattg gaaaataatt tgtatccctg ggtttcctat ggaattttac ctatatttgc
      181 ctttgcaaat gcgggggtat ctttcgaagg attaactttt actatgctgc aatcgcctat
      241 aacactgggc attgcaagtg gtctcttttt tggaaaacaa attggtgtaa tgtctatgag
      301 tgctttagct attattctaa agcttgcgaa ccttccacca aaagtaaatt ggttacaatt
      361 ttatggatta gcgcttataa caggcattgg ttttactatg agtttgttca ttggaacttt
      421 agcgttttat gatttggaaa cccaggcagc ggtacgttta ggtgttttaa ttggctcttt
      481 attttctgcc attttgggtt gtattatcat tgtcatagca aataagcgtt ctattgttcc
      541 atcaattcaa actggtacat cctaattaca atcgtaacca ggatgtaata atacataact
      601 tttataacgc taatttcgcc cttgtggaag gttggttcaa cctatcctta ataattccgt
      661 ttaaaatatc gtagatttca ctataacaat attttttgca ttgcttagct agatcaactg
      721 caaactgctc atagttttta taggattggc cccacattag aggttgttcc gtgatccgtg
      781 cactaccaaa ccagaattta gcatcttcta taaataaagc aaccgcatca tccgatattt
      841 ggaaaagagc attttgttca tccgataaag gaatgcattc ctcaatttct aaatcaagag
      901 cattttttaa acctcttaat tgctcaagac taaaattatt gaattggtag aataaattat
      961 ttttaattgc aggattttcc gataataatt gttctaaaaa ttttcgtcta aatatactcg
     1021 gagtaagttc gcgagattcg ggtttttgtg caagcgggtg ttgaaaagcg gattggatta
     1081 aacgacggct caccccaata gcgcaagtag gcttatcttc cttttcatcg tcatattctt
     1141 ctagcaatcc gtggtgattt ctgcgcttag catcccaatt gtgtgcccga gcgattagag
     1201 caatttctcg tgtaaaacac tctttaagat cttggttaga aatattattt tctagtatta
     1261 tggtatcatc agagacagca agccacaaat acgccaaatt ttctttatct tcatcagcaa
     1321 tagctgctgc tctgccattt ggcaagatat cagcaaaagg catatctttt aatatccacg
     1381 ggtttggtct taaaaaatag cgccaaccgg tgtgccaaat atttttataa tatggttttt
     1441 gctccatagc gccattataa ggctctaaag gtaatttgtt acctttcgca tcgaaggcag
     1501 gtgtatgatc atattgagat ttcaaaaagt cttgataatt tgcaaggata gtattttgac
     1561 ctaaagtacg aaatttttca ccataatatt ttttaatatt aaccaataga cccaattctt
     1621 gattatctag tgctatagtt gcgttctcat tgtctgcagc tatttgatgt agattaatct
     1681 gttgtaaatc ttctgcatta gcaaaatcgc gcaccgcagc aatactaatt aacctctcaa
     1741 taatttgtct ataattattg gctcgcgcaa ttcggaatgc ctcattgtta ttcatcgtta
     1801 cgttatctag aatgttaggg aattctaaaa gacgattcac taattcaaaa tcacccactt
     1861 gacaagctaa aagtaaaaca ttgttagaaa gtaaaatttg tggtgtattg ggtgcgttac
     1921 gtaccgcgga catggttaat agtgatttaa tgcaggcaga tttacgaatg cttgctgcaa
     1981 gcgccaaagg gttcacacca tgtaaatcag acattgtaaa atcaattgtg ttttgattca
     2041 ttaacaattc aaatatttct ttatggtaaa aacgtatagc aatgtgcata atcgtttcgc
     2101 catggttatt aactgaattt aagtttaaat ttgcattctt taacaaagca gtaacagcat
     2161 ggatattgtg tgattttacc gcgtaaaaaa gagcattttc tccgcgttta tcctttaggt
     2221 ttggatcaat attggatgat ttcaacaatg tgtcaactgc ggagtcatat cctagtttgg
     2281 ctgcatagtg taaaggcgtt attccctcat tattttgtaa gtttacaagg gctgcctctg
     2341 agattaaatg ctcaaccatt tcaaaattga gttctaataa agcacaatgc aagggcgtgt
     2401 taccatctaa atttttttca tttacatcag cattattgtt aatcaataat ttagtcaatg
     2461 tattttgatg gttccgtaat gcataatgga gtaaggattc accgtttgga aatagaatat
     2521 tcacatcagc acctccgtct tgaatgaaaa gttttgcatt ggctaatctg ttgtttaaga
     2581 ctgccaaatg aagaggcgtg aatccttcgt ttgaaagttt attaatagca gttttacttt
     2641 ttgtgagcac caattttgta attgcactgt tcgtttggtt ggcacagtaa tgtaaaggta
     2701 atttattttt gttgtcagct gtattaacgc atgcgtgatc agattctaaa aaaagcttta
     2761 ctatttcaaa ttgtgattca cttattgcaa gatgaagtgg tgtatgtccc cacaaatcac
     2821 ttgtgtttaa tagatcaaag ttactatcga ttaacatttt ggcgatatta ctcctcccct
     2881 tttctgctgc aacatgcaat gcattttgtt caaatcgatt ttttattctt ggatctgact
     2941 ttccgtcatt cagtaaaaaa taaacaagtt caggttttgc aagcttggta gccaaaatta
     3001 gaggagtgtc accttggcta tctactgcat taacatttgc gtttccttca tttactagga
     3061 actttacaat atccgcccta tcatttttta aatataaact ttgaagtaag gctgaccaaa
     3121 ttgtcgcaaa acgcggttta tgatgaagaa tagctaaatg taagggggtt tggcgtttgt
     3181 tatttttagc ttcaatattt gcgccttcac gtattaattt tctaacccca tgattagagc
     3241 catgctctat agctcgatgt aataaggtat tacctttttt atctgtatta ttaatattta
     3301 aagtagaatt tgcttgatta ggatgctgca tcttatttca tattgtaaac tgaggatgca
     3361 ataaaatacc atgattcctt aaagtagtta gaatgtaacc gataaccggt gcattatttt
     3421 tttaaatggt acttcgttag acggattatt tcataaaaat acacttattt gtgataagtg
     3481 tatttttagg aagttggatt atttaaaaaa ggacacattt cgctgcaata tggctatttt
     3541 ggagtaccgc ccttttttgc aactttgcga atccaatggg ttgtgcattg cccagaaaac
     3601 gaattactct aacttgcccc ttgggctgtt gttgcgcttg aaattttttc ttcaatttat
     3661 tttgctcagc ttgccttttt agatcagcca tacgctgtag ctgttgcgct cgcagctgtt
     3721 cttgtcttct ttgttc
//
LOCUS       DASTIB010000132         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_614418, whole genome shotgun sequence.
ACCESSION   DASTIB010000132 DASTIB010000000
VERSION     DASTIB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614418"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFP93_02280"
     CDS             <1..220
                     /locus_tag="VFP93_02280"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843470.1"
                     /translation="SVLQMEGEVNEVEAKIDAWMLHHKVLVDRFKQLVSDLKAQPSRT
                     FTMYSVALRELLDLSQASQVSILSGKYN"
     gene            221..1210
                     /locus_tag="VFP93_02285"
     CDS             221..1210
                     /locus_tag="VFP93_02285"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET9843471.1"
                     /translation="MYTLAKPLLFNMDPERAHTFALKSLPIYAKLFGDKPYAKPVQVM
                     GLSFPNPVGLAAGLDKNGAFISAWFKLGFGFVEIGTLTPQPQTGNPKPRLFRLVEDKA
                     IINRMGFNNNGIQHFLKMHKRKQYPGILGINIGKNKTTPLAEAIEDYRVGFEQVYSIA
                     DYITLNVSSPNTEGLRQFETGEYLNALLLTLKESQKKCEELYNKKVPLVVKISPDLND
                     AELRNTAIQIKNNRVDGVIATNTTVSRPSLQSCYRMESGGLSGAPLKERSIKIIQKLQ
                     HFFEDQIPIIGVGGIFNLQDAKAKLDAGAKLIQVYSGLIYQGPELIKLLVRGI"
     gene            complement(1420..>2119)
                     /locus_tag="VFP93_02290"
     CDS             complement(1420..>2119)
                     /locus_tag="VFP93_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9843472.1"
                     /translation="HAAKIKDTLSDFYQQVLDLEYTGQSAASFIHPTPFKNLYILPAT
                     HELENLLHPLTTRNKLYKLRHLLDSLDPFDNIFMDTPPALNFYTQSALIAGDSCLIPF
                     DCDLFSRNAIYNLIRGVTEIRAKYHANLRVEGVIVNQFQPSASLPQKLVDELKVEGLP
                     ILSTYITQSVKIRESHDLAMPMIHYEPKHKLTSEFVRIFQSLQTNSIVQPDALPKKRT
                     RKPKKSKLVAPETA"
BASE COUNT          669 a          366 c          446 g          638 t
ORIGIN      
        1 atctgtttta cagatggaag gagaggtgaa tgaagtagaa gctaaaattg atgcttggat
       61 gttacaccac aaggtactcg tcgatcgatt taagcaattg gtaagtgatt tgaaagcaca
      121 accgagccga acttttacta tgtactcggt agcattgcga gagttgttag atttgtcgca
      181 agcatcgcag gtaagtattt taagtggcaa gtataattaa atgtatacat tggctaagcc
      241 tttgctattt aacatggatc cggaacgagc ccataccttt gcgttgaaaa gcttgcctat
      301 ttacgcaaaa ctatttggcg ataaacctta tgcgaaaccg gttcaagtta tggggttatc
      361 gtttccaaat cctgtaggcc ttgcagccgg tttagataaa aatggtgcgt ttatttctgc
      421 ttggtttaaa ttaggctttg gctttgtgga aataggtact ttaactccgc aacctcaaac
      481 agggaatcct aagcctaggc tatttcgctt ggttgaggac aaagcgatta ttaatcgcat
      541 gggttttaat aataatggca ttcagcattt cttgaaaatg cataaaagaa agcaataccc
      601 cggtattcta gggatcaata ttggaaaaaa taaaactacg cccttagcgg aggcaattga
      661 agattataga gtaggttttg aacaagtata ttcgattgca gattatataa ccttgaacgt
      721 ctcttcgccc aacacggaag gcttacgcca atttgaaacc ggcgaatatt taaatgcatt
      781 attattaact ttgaaagaaa gtcaaaaaaa gtgtgaagaa ctatataaca aaaaagttcc
      841 cctggtggta aaaatctctc ccgatctcaa cgatgctgaa ttacgtaata cggcaataca
      901 aataaaaaat aaccgcgtag atggcgtgat agcaacgaat acaaccgtgt cacgtccgtc
      961 tcttcaaagt tgttatcgta tggaatctgg tggtttaagt ggtgcaccgt taaaagaacg
     1021 ttcaataaag attatccaaa aattgcaaca tttttttgaa gatcaaattc ctatcattgg
     1081 cgtaggtgga atttttaacc tgcaagatgc aaaagcaaaa ttggacgctg gagcaaaact
     1141 tatacaggtg tacagtggtt taatttatca aggcccagag cttattaaac tgcttgttcg
     1201 aggaatttag aagtttgacg gatcccctaa gtcgcaactt gcgaaaaagt tgtcgtcatc
     1261 gcggaattta gcaacatagc aagcttgcga gcgttatgtt gcttaatatc cgcgatccat
     1321 tttagatatt taaagatttt aagatggatc tcgctttcgc gagaaagaca gaagatacat
     1381 atccgcattc catttaaagt tgcggcacat gaaagaccat taggcggttt caggagctac
     1441 caatttgctt ttcttcggtt ttcgcgttct ttttttcggt aatgcatcgg gttgcacgat
     1501 cgaattggtt tgcaaagatt gaaatattct tacaaattct gaagtgagtt tatgcttagg
     1561 ttcgtaatgg atcatcggca ttgccaaatc atgcgattct cggattttaa ccgattgggt
     1621 aatataagtg cttaaaatag gcaaaccttc cacttttaat tcatccacca atttttgcgg
     1681 caagctcgct gaaggttgaa attggttaac aattacgcct tctacccgta aattagcgtg
     1741 gtattttgca cgaatttctg taacgcctcg aattaaatta taaatagcat tccgtgagaa
     1801 taagtcgcaa tcaaaaggta taagacagct atcgcctgcg attaaggcag attgggtata
     1861 aaaattcaaa gcaggtggag tatccataaa gatattgtca aatggatcca aactatctaa
     1921 taagtgtcgc aatttataca atttattacg cgtagttaat ggatgcaata aattctctaa
     1981 ttcatgggta gcaggcaaaa tgtaaagatt tttaaaaggt gttggatgaa taaaacttgc
     2041 ggcggattgg ccagtatatt ccagatctaa aacctgttgg taaaaatcgc tcagagtgtc
     2101 tttgattttt gcagcatgc
//
LOCUS       DASTIB010000133         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_837639, whole genome shotgun sequence.
ACCESSION   DASTIB010000133 DASTIB010000000
VERSION     DASTIB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837639"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2543
                     /locus_tag="VFP93_02295"
     CDS             <1..>2543
                     /locus_tag="VFP93_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843473.1"
                     /translation="QIIKTEASKLNEVHQTNLLDIVGAKAQEEYMLLKGRLHLYLRDA
                     QNAEKQFYEASLIKIIQDRRQRLENYIFSHYKQSFQTIKNFYLKNIDGILQQEPILQK
                     ADSDLIAYIELIKLQLIKLTNEINTVFNSNHYFTFLDDHIKKYNTTLYSKFVNYFFSI
                     IKDKVEPLLFRKGNSQIEILLLDTDGFLASFNTLNAVIEDKTIQFNATHVSPAPNSIL
                     DTVQLHNSLNHLIFLKDYIDNVTISSKDLMEKVNNVSLKFSYTEKLLRLCMQVENLIK
                     KFNLPIESSFIFNGKLWFEEPNKSLLLADLTRDNCYNIFKNLRDEFTTKKQYNNIVWI
                     KNFILIESILQTITISVLLDAAVLPLDTYFQLSKLLQEKFKETELDTGKALDFYAHHM
                     LSQGEQLLQNLQTKFEQEDILFENPVHALRRVHYEPIAISTTSGKQKKAFGSGFYQNQ
                     GTKYYLKHVTNANYVADDIVEVLVSNLSSYFQQGHLFAQCHLVKVDNNEVFLASRCEK
                     DFTPLVNTDERLISRLTKEEVTNLMTALTEDKRKQLINILAFALLWGDYDCNPSNIGI
                     EPVQKIDHGWAFENICREQPMIIEEELDFLRYLDRFAPTNTFRDYKAFLRTSSFFDSV
                     KELSNRFNLQEIRDLVNNTLSRVEHIYHKSKAPISLDKRLKKAECSFPGIQGFYTKKE
                     IRYSICERVGLESNTDDLNYLANKITEALFIRLQVLKLYCIRNRENQEKVNLSSIQDI
                     RDILLETQEKIRGISLVAYFPELRAMLLDLYDAFSQNAFQDKTMLTMVKQCALLASID
                     IEDKNYDVEPSYAAVNNHFLTDYALKKVKRSKEGGLPGSSTKKQKFNAN"
BASE COUNT          904 a          433 c          429 g          777 t
ORIGIN      
        1 acaaatcatt aaaacggaag cctcaaagct taacgaagtc caccaaacta acctgctaga
       61 tatagttggc gcaaaagcac aagaagagta tatgctccta aaaggacgct tacatttgta
      121 cttgcgggat gcccaaaatg ctgaaaaaca attttatgaa gcatcgttaa taaagataat
      181 ccaagatcga agacaacggc tcgaaaatta tattttttca cactataaac aatcttttca
      241 aactattaaa aacttttatt taaaaaatat tgatggaatt ctccaacaag aacctatttt
      301 gcaaaaagcc gattccgatc ttatagccta cattgaattg attaaattgc aattaattaa
      361 attaacgaat gaaataaaca cggtattcaa ttcaaaccac tattttactt ttttagatga
      421 tcatattaaa aaatataata caaccctcta tagtaagttc gtaaattatt ttttctctat
      481 tataaaagat aaagttgagc ctctcctgtt ccgcaaaggt aatagtcaaa tagaaatcct
      541 gttattagac acggatggct ttttggcttc ttttaatact ttgaatgcgg tcatagaaga
      601 taaaaccata cagtttaatg caacacatgt atcacctgcg ccaaactcta tactagatac
      661 ggttcaactc cataatagcc tgaatcactt aattttttta aaagactata tagataatgt
      721 tactatctct tcaaaagact tgatggaaaa ggttaataat gtttccctaa aattttctta
      781 caccgaaaaa ttattacgcc tttgcatgca ggttgaaaat ctaattaaaa aatttaacct
      841 accgattgag tcgagcttta tatttaatgg gaaactgtgg tttgaagaac cgaataaaag
      901 cttattgctc gctgatttaa cccgtgataa ttgttataat atttttaaaa atttacggga
      961 tgaattcaca acaaaaaagc aatataacaa tattgtttgg attaaaaatt tcattctaat
     1021 agaatctatt ttacaaacta tcaccatttc tgttttatta gatgcagcag tactacccct
     1081 cgatacgtat ttccagctta gcaaactctt acaagaaaaa tttaaagaaa ccgaattgga
     1141 tacgggcaaa gctttggatt tttacgcgca tcatatgtta agccagggtg aacagctgtt
     1201 gcaaaatcta caaacgaaat ttgagcaaga agacattttg tttgaaaacc ccgtgcatgc
     1261 attacgtcgg gtacactacg agcctattgc tatctcaaca acgagtggca agcaaaaaaa
     1321 agcatttggc tctggatttt accagaatca agggaccaaa tattatttaa aacatgtcac
     1381 aaatgctaat tatgtagccg atgatattgt agaagtgttg gtgtccaacc tatcttccta
     1441 ttttcagcaa gggcatcttt ttgctcaatg tcatttagta aaagtggata ataatgaggt
     1501 ttttttggcc tctaggtgcg aaaaagattt tactcccttg gttaatacgg atgagcgctt
     1561 aataagtcgt ttaactaaag aggaggtgac aaatttaatg actgccttaa ccgaagataa
     1621 gcgcaaacaa ttgataaaca ttttagcctt tgcacttttg tggggcgatt acgactgtaa
     1681 tccgagcaat attggtatcg aacccgtgca aaaaattgat catggttggg cttttgaaaa
     1741 tatttgtcgt gaacaaccca tgataattga agaagaatta gattttttgc ggtatttaga
     1801 tcgatttgca cccaccaata cgtttagaga ttataaagcg tttttgcgca catcctcgtt
     1861 ctttgacagc gtaaaagagc tttcaaatag atttaacctg caggaaatac gcgatttggt
     1921 taacaatact ttatcaaggg tagaacacat ttatcataaa tcgaaagcgc caatatctct
     1981 agataaacga ttaaaaaaag cggaatgctc tttcccaggt atacaaggat tttatacgaa
     2041 gaaagaaatt cgttatagta tttgtgaaag agtgggactt gaatctaaca cagatgatct
     2101 aaattatctg gcaaataaaa taaccgaagc actgtttata cgattgcaag tgttgaaatt
     2161 gtactgtatt cgaaatagag aaaatcagga aaaagttaac ttaagtagca ttcaggatat
     2221 acgtgacatt ctcttagaga ctcaggaaaa aatacgcgga atttcgttgg tggcctattt
     2281 tcctgaatta cgcgcaatgt tgttggattt atatgatgct ttttcccaaa atgcattcca
     2341 ggataaaaca atgcttacca tggtaaagca atgcgcccta cttgcttcta tcgatattga
     2401 agataaaaac tatgacgtag agccttcata cgctgcagtc aataaccatt ttcttaccga
     2461 ttatgcttta aaaaaagtga aaagatcaaa agagggtggc ttacctggat cttccacaaa
     2521 aaagcaaaag tttaatgcaa ata
//
LOCUS       DASTIB010000134         5336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1386416, whole genome shotgun sequence.
ACCESSION   DASTIB010000134 DASTIB010000000
VERSION     DASTIB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386416"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1177)
                     /gene="sucC"
                     /locus_tag="VFP93_02300"
     CDS             complement(8..1177)
                     /gene="sucC"
                     /locus_tag="VFP93_02300"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET9843474.1"
                     /translation="MNLHEYQAKELFASYGLPVANNVVIRSVHEIPYALEVLGTSHVV
                     VKAQVHAGGRGKAGGVRLVKDRDSLTEAARDLLHKRLVTHQTTQKGQPINELLIEAVT
                     EIDSEMYLGMLVDRLAQTITVMISKEGGVDIEKVAAETPEKILKFHIHPQLGVMPYQV
                     RDMGFHLGLSMQQIKQLTTIVQGLGKMFTEKDLSMVEINPLVITQSSDLLCLDAKVNV
                     DENALYRQTDLRDLRDSTQEDEREIQAREWELNYIALDGNIGCMVNGAGLAMATMDII
                     KLHGGNPANFLDVGGGATRERVAEAFKIILSDQNVKAVLVNIFGGIVRCDLIAEGIIG
                     AVQEVGIQVPVVVRLEGNRAVEASKILEKSGLNITPVQDLTQAAVEVVKLAGAKA"
     gene            complement(1189..2418)
                     /gene="odhB"
                     /locus_tag="VFP93_02305"
     CDS             complement(1189..2418)
                     /gene="odhB"
                     /locus_tag="VFP93_02305"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET9843475.1"
                     /translation="MSSEIKVPVLPESVSDATVGKWHKNPGERVTRDENLVDIETDKV
                     VLEVVAPADGILEEIVKASGETVGAQEVIAKFKSIKADAKASKKSSAQEEKPKEINQE
                     KKMETTSKPKKEEVQASPSVRRLIAEHDLDVDKISGSGKKGAITKKDVEQAIHSESGN
                     GSQTQHQLKTFTVNAEERSEKRVPMSRLRAKIAERLLSAQQNAAILTTFNEVNMKPIM
                     DLRTKYKESFEKAHGIKLGFMPFFVKAVIEALKRFPAVNASIDDKDIIYHGYFDIGVA
                     VSTERGLVVPVLKNADLLSMAHIEETIAQLGKKARDGQLSIEEMTGGTFTISNGGIFG
                     SLLSTPILNPPQTGILGMHKIEERPIVENGEIVIRPMMYVALSYDHRLIDGRESVQFL
                     VTIKNLLEDPARMLLEV"
     gene            complement(2431..5268)
                     /locus_tag="VFP93_02310"
     CDS             complement(2431..5268)
                     /locus_tag="VFP93_02310"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010180455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET9843476.1"
                     /translation="MQQASTMKEMWASSALAGSNASYIEELFEKYLEDSESVPYEWQE
                     LFNNLPKIKGGDGDVSHQAILQDLKHIASHKKISLITPQAKDYHSVTLEHERKQIQVL
                     RLIQAYRLQGHLHAQINPLGFRELETVYSTELSLEDHGLSVNDLGITFDVETFVGPKQ
                     MTLGQLYYALTNTYCGSIGTEYMHIPYQEEREWIQHRIEAARGAPIYTNEVKVKILER
                     LIAAEGLEKYLGSKYPGAKRFSLEGGDALIVILDELIQRAGTHKAREVIMAMAHRGRL
                     NVLINVLGKKASDLFDEFEGKYFANLESGDVKYHQGFSSDIATPGGNVHVALAFNPSH
                     LEIVTPVMCGSVRSRQKRLNDPDHNKVLPIAIHGDAAFSGQGVVMETLNMSETRAYRI
                     GGTVHIIVNNQIGFTTSIRQDARSTLYCSDVAKVVLAPIFHVNADDPEAVLFVTQLAL
                     DYRMRFNKDVVIDLVCYRRHGHNEADEPAATQPKMYKKIRKHPTVVEVYAKNLILQGI
                     LTEEDVEHHVEKNRARLDKGECVASNIVTGLVSDYAIDWTPYFSFDWRAKVKTKIDKS
                     TFQRIAEQMVKLPENFELHPRVKKIFEDRKRMGQGEILCDWGFAETLAYASLCDQCIQ
                     VRITGQDVGRGTFFHRHAILHDQKTGEVYIPIANVNSNQGKFEIYDSLLSEEAVLAFE
                     YGYSTTDPSALVVWEAQFGDFANGAQVVIDQFISSGQQKWGRLCGLVMLLPHGYEGQG
                     PEHSSARLERFLQLCAELNIQVCVPTTASQVFHMLRRQILRKIRTPLIVMSPKSLLRH
                     KEASSDIQEFIHGEFKLIIPENHDLDKDKVTKIVLCAGKVYYDLREERNKKEKFYIAI
                     IRIEQLYPFPEDELRKEIAQYKNVEVVVWCQEEPKNQGAWYSQQHHFISCISNKQTLV
                     YAGRPASAAPAVGYLKAHTEQQSALIEEALA"
BASE COUNT         1459 a         1179 c          973 g         1725 t
ORIGIN      
        1 aaaatactca tgcttttgct ccagccaact tcaccacttc tactgcagcc tgagttagat
       61 cttgtacagg ggtaatatta agcccacttt tctctagaat tttacttgct tcaaccgcac
      121 gattaccttc taaacgaacc accacaggaa cttgaattcc tacttcttga accgcaccaa
      181 taatgccttc tgcaatcaaa tcgcaacgta caataccacc aaaaatattt actaaaacag
      241 cttttacatt ttgatcggat aatataatct taaacgcttc cgctacccgt tctctggttg
      301 ctccacctcc aacatccaag aagtttgcag gattgccacc gtgcagttta ataatgtcca
      361 tcgttgccat agcaagccct gccccattca ccatacaccc gatattgcca tctaaggcta
      421 tataattaag ttcccattct cgggcttgaa tttcgcgttc gtcttcttgg gtagaatcgc
      481 gcaaatcgcg tagatcggtt tgacgataaa gtgcattttc gtcaacattc actttggcat
      541 ctaaacataa taaatcggaa gattgggtta tgacgagcgg attaatttcc accatgctta
      601 agtctttttc tgtgaacatt tttcctaagc cttgaacaat agtggttagt tgcttaattt
      661 gctgcataga caagcctaaa tgaaatccca tatcccgcac ctgatacggc ataacaccaa
      721 gctgtggatg tatatgaaat tttaaaattt tttcaggagt ttctgctgca actttttcaa
      781 tatccacgcc accttctttg gatatcatca cagtgatggt ttgtgctaat ctgtctacta
      841 gcatacctaa atacatttcc gagtcgattt ccgttaccgc ttcgatcaat aattcattga
      901 taggctggcc tttttgcgta gtttgatggg ttacaaggcg tttgtgcaat aaatcccttg
      961 ctgcctctgt gagcgaatct ctatctttta cgagtctcac accgcctgct tttcctctac
     1021 ctcccgcatg tacttgcgct ttgaccacta catgggaggt acctaaaact tctaacgcat
     1081 aggggatctc gtggacagaa cgtattacca cattattcgc aacgggtaag ccataagatg
     1141 caaataattc ttttgcttgg tattcatgta aattcatagg tttttcgctt atacttcgag
     1201 caacattctt gcaggatctt ctaataaatt tttaattgta accagaaatt gcacggattc
     1261 tcttccgtca attaatcggt gatcatagga caacgcaaca tacatcatag gtcgaattac
     1321 aatttcacca ttttcaacaa tgggcctttc ttctattttg tgcatgccta aaatacctgt
     1381 ttgcggtgga ttaagaatgg gtgtggaaag aagagaacca aaaattccac cattggaaat
     1441 tgtaaaggtt ccccctgtca tttcctcaat agacaattga ccatcacgcg ctttttttcc
     1501 taattgagca atagtttctt cgatgtgtgc catcgagagt aaatcggcat tttttaatac
     1561 agggacgact aagcctcttt cggtactcac cgccacacca atatcaaaat agccatgata
     1621 aataatatct ttatcatcga tagatgcatt cacagcagga aaacgtttaa gtgcttcgat
     1681 cacagctttg acgaaaaatg gcataaaacc taatttgatg ccatgggctt tttcaaacga
     1741 ttctttatat tttgttcgca aatccataat aggtttcata ttgacttcat tgaacgttgt
     1801 taagatcgcg gcattttgtt gtgcactcag taaacgttct gcaatcttag cgcgcaaccg
     1861 agacatcgga acgcgctttt ccgatcgttc ttcggcattt actgtgaatg ttttcaattg
     1921 atgttgcgtt tgtgacccat tgccagattc tgaatggatc gcttgttcca cgtctttttt
     1981 cgtgattgca cccttttttc ccgaaccaga aattttgtca acatcgagat catgttcagc
     2041 tataagacgg cgcacagaag gtgaggcttg gacttcttcc ttttttggct tagaggtagt
     2101 ttccattttt ttttcctgat taatttcttt aggtttttct tcttgtgcag aggatttttt
     2161 acttgcttta gcatctgctt ttatggattt aaacttcgcg ataacttcct gggcgccaac
     2221 cgtttctccg ctggctttaa caatttcttc caagatacca tcggcgggtg ctaccacttc
     2281 taatacaacc ttatcggttt caatatctac taaattttca tcgcgtgtaa cacgttctcc
     2341 tggattttta tgccatttgc ctacggtggc gtcacttacc gattccggta atacgggtac
     2401 tttaatctca ctgctcatac tttttccttt ttaagctaag gcttcttcaa ttaaagcgga
     2461 ttgttgctct gtatgcgctt tcaaatatcc taccgcagga gctgcagaag caggacggcc
     2521 cgcatatact aaagtttgct tattggatat acaactaata aaatgatgtt gctgtgaata
     2581 ccatgcgccc tgatttttgg gctcttcctg acaccacaca acgacttcaa catttttata
     2641 ttgagcaatt tctttacgca gctcatcttc gggaaaagga tacagctgct caatacgtat
     2701 tatagcgata taaaattttt cttttttatt tctttcttcc cttagatcat aataaacttt
     2761 accggcacac agcacaattt tcgttacttt atctttgtcg aggtcgtgat tttcaggaat
     2821 aattaattta aactcaccat gaataaattc ctgaatatcc gaagaagctt ctttatgccg
     2881 cagtaaactt ttcggggaca tcacgataag gggcgtgcgt atcttgcgta atatttgtcg
     2941 ccttaacata tgaaaaacct gactagctgt ggtgggcaca cacacttgaa tatttaattc
     3001 agcacaaagt tgtaaaaaac gttctaaacg ggcagaagaa tgctcggggc cttgaccttc
     3061 atagccatgg ggtaaaagca taaccaaacc acataatcgt ccccactttt gttgtcccga
     3121 acttataaac tgatcgataa caacttgcgc gccattagca aaatcaccaa attgtgcttc
     3181 ccaaacgacc aaagcggaag ggtctgtggt ggagtatcca tattcaaacg caagcactgc
     3241 ttcttcagag agtagtgaat cgtatatttc gaatttgcct tggttactgt taacatttgc
     3301 gatgggaata taaacttcgc cggttttttg atcgtgcagt atggcgtggc gatgaaaaaa
     3361 tgtgccacgc ccaacatctt gtcccgtaat ccttacttgt atacattgat cgcaaagaga
     3421 agcatatgcc aaagtttcag caaaacccca atcacacaaa atttcgcctt gtcccatccg
     3481 ttttctatct tcaaatattt ttttaacgcg cggatgtaat tcaaaatttt ctggcaactt
     3541 aaccatttgc tcagcaattc tctgaaaagt tgatttatcg atcttggttt taactttagc
     3601 gcgccaatca aaggaaaaat agggtgtcca atcgatagca taatcggaca cgagcccggt
     3661 tacgatattg gaggcgacac attctccttt gtctaagcgc gcacgatttt tctctacgtg
     3721 atgttccaca tcttcttctg tcaaaattcc ttgaaggatt aaatttttcg catacacttc
     3781 tactactgtt gggtgtttgc gaattttttt atacatttta ggttgggttg ctgctggttc
     3841 atcggcttcg ttatggccat gacgacgata gcacactaaa tcaataacaa catctttatt
     3901 aaatcgcatg cgataatcga gcgctaattg ggttacaaat aataccgctt ctggatcgtc
     3961 ggcattcaca tgaaagatag gcgctaaaac gacttttgcc acatcactac aatataaagt
     4021 agatcgagca tcttgcctta tgcttgtggt aaagccgatt tggttattga ctatgatatg
     4081 taccgttcca ccaattcggt aggcgcgggt ttcagacata ttcaacgttt ccattaccac
     4141 accttggcca gaaaaagcag catctccatg aatcgctatc ggtaaaactt tattatgatc
     4201 cggatcgttt aatctttttt ggcgggaccg aacggatcca cacatcaccg gagtaacaat
     4261 ttctaaatgc gatggattga acgcaagtgc tacatggaca tttccacccg gggtcgcaat
     4321 atcggacgaa aatccttgat ggtatttaac atctccagat tctaaattcg caaaatattt
     4381 cccttcaaat tcatcgaaga gatcacttgc ttttttccct aatacgttaa ttaatacatt
     4441 taatcggcct cggtgggcca tagccattat aacttcacgc gctttatgcg tgcctgcgcg
     4501 ttgaattaat tcatcgagaa ttacaatgag cgcatcgcca ccttcgagtg aaaatcgttt
     4561 agctcctggg tatttggatc ctaaatattt ttctaaacct tcggctgcaa ttaaacgctc
     4621 tagaatttta acttttactt cattcgtata aataggagca ccacgtgccg cttctatgcg
     4681 atgttggatc cattcccgct cttcttgata aggaatatgc atatattcag taccgataga
     4741 accacaatag gtatttgtca gcgcataata gagctgaccg agcgtcattt gttttggacc
     4801 tacaaaagtt tctacatcaa aggtgatccc taaatcattc actgaaagac catgatcttc
     4861 taaactgagc tcggtagaat aaaccgtttc taattcgcgg aaacctaaag gattaatttg
     4921 ggcatgtaag tgaccttgta aacgataggc ttggatgagg cgcaaaactt ggatctgttt
     4981 tctctcatgt tctagcgtta cactgtgata atctttagct tgtggagtaa ttaaactaat
     5041 ttttttatgg ctggcaatgt gtttgagatc ttgtaaaatt gcttgatggg atacatctcc
     5101 atcgccacct tttattttag gtaggttatt gaataattct tgccactcat acggtacact
     5161 ttcggagtct tcaagatatt tttcaaaaag ttcttctata taacttgcgt tgctgcctgc
     5221 caaagcagag gacgcccaca tttctttcat cgtgcttgct tgctgcataa tcacacccaa
     5281 tccatcttag gaaaaaatgt atctgtggaa aatttcagag tgaagattag ttatta
//
LOCUS       DASTIB010000135         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_350838, whole genome shotgun sequence.
ACCESSION   DASTIB010000135 DASTIB010000000
VERSION     DASTIB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350838"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="VFP93_02315"
     CDS             complement(<1..2130)
                     /locus_tag="VFP93_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003779 - actin binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WH2 domain-containing protein"
                     /protein_id="HET9843477.1"
                     /translation="MSFKEILRFFRALEQGIFAAKPKDNDIDVEAVENEDSIEAVVSH
                     KHTIPVIKHKQAFSLAESIPKSLNIVNKKFEDPVDKEPLARVMEKNSKLHHKHGLSNF
                     EFNTKFIKATEVKITDLDKKAVQHISISLPEEHEFIKGKLNTCVDAIQNFAQELIKQI
                     TMPTKPVSINIEFQEEYELINKEKLNNLVNKIQEFSHNLINQMVDNKSEPKIELETQA
                     THNEEYELVDKEKLNTIINEIRKFSHHLIEQMVNTKAETSVLNNKPEPKIELKTQEIY
                     NEEYELVNKDKLNALVNDIQKFSHDLIAQIVNANPQPDHNEPAPLEAEIPVPPPLPPK
                     KVLISVPSVQKSNEKSDAEVIHSKGKAKLELDSVAAEKEDDLRQGQQQRQDDNADEIE
                     RNEILRNLMDEIKNFNKDALKKTPVNNEPKAQTPHDALMAQIPAAAKQLKNVADPDSV
                     RAQKIQAIEEGALNRPLNPREELMKAIREHGKINNVQKAEEVYLNDAQLANDLQLPVE
                     TNKVEENKNPLINNNFVEQNEPNPATIVIPALPPLPPKKVMAPTIEVKPNNILLDEIS
                     LNEKSKSDKPVSVDLLNKNESAEDDLRQGQQEQQEEDAEKKMMFADLLDGIKNFDRNG
                     LKKAPVNTAPKAQTAHDALIAQIPAAAKQLKNVADPDMVRAQKIQAIEEGNPNRPLNA
                     REELMKAIREHGKINNVQKAEEVYLNDA"
     gene            complement(2266..>3499)
                     /gene="ligA"
                     /locus_tag="VFP93_02320"
     CDS             complement(2266..>3499)
                     /gene="ligA"
                     /locus_tag="VFP93_02320"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9843478.1"
                     /translation="TLRTLVDDLQGVQVFYQNLLKKRPSLPFEIDGLVIKVNDHFQQA
                     KLGVVSRAPRWAIAYKFPAQEEETQVKNIEFRVGRTGALTPVARLQPVFVGGVTVSNA
                     TLHNMDEIARKDIRINDYVIVRRAGDVIPEVVRSIPEKRPLDAKKIKLPKKCPICGSQ
                     VIKHADYSVAKCSGGLYCLAQVKEAIKHFVSRRAMDIEGLGVKLVENLVDHKLIRNVA
                     DIYQLQREDLLALERMGERSVDKLLKAIAKSKKTTLDRFLYALGIPEVGEATALSLAF
                     HYAALPPIIAAQEEELQSIADIGPIMASHIATFFRQKHNREMIDALLRMGISWPDVSQ
                     TKDTTLAGKTYVITGTLSIPRDELKAKLQKRGAKVSNSVSAHTTAVIVGENPGSKLNK
                     ALELKIPIVTEADLKELL"
BASE COUNT          899 a          686 c          613 g         1301 t
ORIGIN      
        1 cgcgtcattc aaataaactt cttcagcttt ctgaacatta tttatcttac catgttcacg
       61 aattgctttc atcaattctt cacgcgcatt taatgggcga ttaggattgc cttcttcaat
      121 agcttgaatt ttttgcgcac gaaccatgtc tggatctgcc acattcttca attgttttgc
      181 cgctgcggga atttgtgcta ttaatgcatc atgagcagtt tgtgctttcg gcgcagtgtt
      241 taccggtgct tttttcaatc cgtttctatc aaaattttta attccatcta ataagtctgc
      301 aaacatcatt ttcttttccg catcttcctc ttgctgctct tgttgccctt gacgcaaatc
      361 atcttcagcg gattcattct tgtttaatag atcaacagag acaggcttat ccgatttgga
      421 tttttcatta agagaaattt catctaataa tatattattg ggcttaactt caattgtagg
      481 cgccattact tttttaggtg gcaacggtgg tagagcagga atcacaattg ttgcaggatt
      541 tggttcattc tgctccacga aattattgtt aataagagga tttttattct cttcaacttt
      601 atttgtttcg acgggtaatt gcaaatcatt cgctaattgc gcgtcattca aataaacttc
      661 ttcagctttc tgaacattat ttatcttacc atgttcacga attgctttca tcaattcttc
      721 gcgtggattc aatgggcgat taagcgcacc ttcttcaatg gcttggattt tttgtgcacg
      781 gacactgtct ggatctgcaa cattctttaa ttgttttgcc gctgcgggaa tttgtgccat
      841 taatgcatca tgtggggttt gtgctttcgg ctcattgttc acaggcgtct tttttaatgc
      901 gtctttgtta aaattcttaa tttcatccat taaatttctt aatatttcat ttctttcaat
      961 ctcatccgca ttgtcatctt gtcgctgttg ctgtccttga cgcaaatcat cttccttttc
     1021 tgctgcaacg ctatccaatt ctaattttgc ttttcctttg ctatgtatta cctctgcatc
     1081 tgatttctcg ttactttttt gaactgaagg tacagatatt aaaacttttt tgggtggtaa
     1141 cggtggagga actggaatct ctgcttctag aggagcgggt tcattatgat caggttgggg
     1201 atttgcattt acaatctgag caattagatc atgagaaaat ttttgaatat cattcactaa
     1261 agcatttaac ttgtctttat tgaccaattc atattcttca ttatatattt cttgcgtttt
     1321 aagctcgatt tttggttcag gcttgttatt caaaactgaa gtttcagctt ttgtatttac
     1381 catttgctca atcaaatgat gagaaaattt tctaatctca ttgattatag tatttagctt
     1441 ttctttatcg accagttcat attcttcatt atgcgttgct tgcgtttcaa gctctatttt
     1501 tggttcagac ttgttatcta ccatttgatt tatcaaatta tgagaaaatt cttgaatttt
     1561 atttactaaa ttatttagct tttctttatt gatcaattcg tactcttctt gaaattcgat
     1621 attgatcgaa acaggcttgg taggcatagt tatttgtttt atcagttctt gggcgaaatt
     1681 ttgaatcgca tccacacaag tatttaactt ccctttaata aattcgtgct cttcggggag
     1741 actaattgat atgtgttgga cagctttttt atctaaatcg gtaattttca cttccgtagc
     1801 tttaataaat tttgtattaa attcaaaatt agataaacca tgtttatgat gcagtttgga
     1861 gttcttctcc attacacgcg ctaaaggttc cttatctact ggatcttcga attttttatt
     1921 tactatgttt aagcttttag gaatgctttc ggcaagcgaa aatgcctgct tatgcttgat
     1981 tacgggaatg gtatgtttgt gcgatactac ggcttcaata gaatcttcgt tttcaactgc
     2041 ttcgacatct atatcattat cttttggttt agccgcaaat attccttgtt ccagcgctct
     2101 gaaaaagcgc aatatttctt taaagctcat tttttaacct ttacccaaat aattaagtta
     2161 tgaaaaacac tataaggtta aggcaatttc cttaaaagaa atattcgatg aaatctaaaa
     2221 tctaccgact gaatggtctg tatgaacaat tactcactta aaatttcaga gtaattcttt
     2281 taaatctgct tctgtaacaa tgggaatttt taactccaaa gctttattta atttactgcc
     2341 ggggttttca ccaacgataa cagcggtggt atgcgcagat acactattag agactttagc
     2401 tcctctcttt tgcaattttg cttttaattc gtctcttgga atactcaggg tacctgttat
     2461 tacatacgtt tttcccgcga gtgtggtgtc ttttgtttgc gatacatccg gccaggatat
     2521 acccattcga aggagcgcat ctatcatttc gcgattatgc ttttgtctaa aaaaggtggc
     2581 gatgtgagac gccataatcg gaccaatatc agcaatcgat tggagttctt cttcttgtgc
     2641 tgcaattatg gggggtaatg ccgcatagtg gaaagccaaa gataaagcgg tggcttcgcc
     2701 cacttctggg attcccagtg catacaaaaa acgatcgagg gtggtttttt tactttttgc
     2761 aatcgctttt aatagtttat caacagagcg ctcacccata cgttctaaag ctaataaatc
     2821 ttcgcgttgc aattggtaaa tatccgcaac attacgtatg agcttatgat ctaccaagtt
     2881 ctctaccaac ttcaccccta agccttcgat atccattgct ctgcgcgata cgaagtgttt
     2941 aatcgcttct tttacttgtg ctaaacaata taaacctccc gaacatttgg ctacggagta
     3001 gtcggcatgt tttataactt gggagccaca tatgggacat tttttaggta attttatttt
     3061 ttttgcatcc agcggtcttt tttctggaat tgatcggact acttcgggaa taacatctcc
     3121 tgctcggcgc acaattacat aatcgtttat gcgaatgtct ttgcgcgcaa tttcatccat
     3181 attgtgtaaa gtagcattgc ttaccgtaac accaccaaca aaaaccggct gcaaacgggc
     3241 taccggagtg agagctcctg tgcgtcccac tctaaactca atattcttta cttgggtttc
     3301 ttcttcttgc gcgggaaatt tgtatgcgat agcccatcgt ggtgcgcgtg aaaccacacc
     3361 taattttgct tgttgaaaat gatcgttaac cttaataacc aacccatcaa tttcaaaagg
     3421 caaggaaggt ctttttttta ataaattttg ataaaaaact tgaactccct gtaaatcatc
     3481 gactaacgta cgtagcgta
//
LOCUS       DASTIB010000136         5540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1149016, whole genome shotgun sequence.
ACCESSION   DASTIB010000136 DASTIB010000000
VERSION     DASTIB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149016"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /gene="lptD"
                     /locus_tag="VFP93_02325"
     CDS             complement(<1..1700)
                     /gene="lptD"
                     /locus_tag="VFP93_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HET9843479.1"
                     /translation="MRIIRKTTLCIFLVTGFNLQSALAEQADFQEFNPTEQVFAEPLD
                     SNQYGWVEDPSLLCKGFYHVPVWDVLREDPKWEISADELTMIREGTSTLRGSVQLVYG
                     QRHFFAKELEIFHVEKVLKEIHANSSIIYQDPDLKIQGSSGFWQAENDIIVVYNPHYF
                     YFPRHAHGSARKVTIEEQRKLLLEGASYTTCPPNDPTWKLQGTHIQLNRDTGMGQARH
                     ISLHLKSIPIFYWPYLQFPIDNKRHTGFLYPNVGSTTQSGFELSWPLYWNIAPNYDAT
                     FTPRVLTERGLDLQNELRYLSSFNSGIFQLHFLPGDRKYKAFRAINLLSPPLEIPLLD
                     PRLQGLEHGNDRLGIRFQHATNFSKYWNLAIDYAYVSDDNYFYDLENDLNSASTNKLN
                     QEVNLHYQNIHWHHWWRLQNIDSLHPLLGPITFEEYGRLPQWAFQGIYPKFARFFSFN
                     LEGEATHFTHKNDPFTDDIVTNGERFYLKPALSLPMERIGGYFKPSVKMDYVGYHLNL
                     SPEHERLFFPNHANRAIPIYAIDTGLFFDKFYPNAYQQTMEPRLYYLYVPFRDQSRYP
                     "
     gene            1837..2859
                     /locus_tag="VFP93_02330"
     CDS             1837..2859
                     /locus_tag="VFP93_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9843480.1"
                     /translation="MTDRMHALTHWVSSQFASRTELIPLHNDASFRRYFRFFHQNISY
                     IVVDAPPTKENNEAFIFMTQLLAQQGITVPKVYNFDLQQGFILLSDFGDTLLLDILNA
                     ENADQWYSNSFEILHKLSTFPKDFHPKIPLFNRQHILLELSYFTEWFLTKLLNVTLNV
                     HDKACLYQVFELLINACETQPQIIIHRDFHSRNLMVLSTGMLGTIDYQDAMLGPLTYD
                     LSSLLKDCYVTWDSYRVQAWVKHYYQILCGKMAIKHFSFERFLLCFDWVGLQRHLKVL
                     GIFSRLKLRDNKARYLADIPRVLHYVLNTAREYPEFEKFYYFMQDKVTPRFEAFLQQT
                     GKQNIA"
     gene            2868..3548
                     /locus_tag="VFP93_02335"
     CDS             2868..3548
                     /locus_tag="VFP93_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9843481.1"
                     /translation="MRAMILAAGRGSRLKPLTDTCPKPLVPVANKPLIVYHLETLYTM
                     GVREVVINVSYLREKIIQFLGNGNTFGLTIEYSIEEYALETGGGIYNALPLLGSNPFL
                     LVSSDIFMDYSGVNFPILDVKLAHLFLVPNPSHNAKGDFGLKQGEVVRKRSQDKSYTY
                     ANLALLKPALFQYCQSEAFPLVAVILQAIMHQGVSGELFQGHWSDVGTLESLQKLEDN
                     INQQFVCA"
     gene            3557..4354
                     /gene="djlA"
                     /locus_tag="VFP93_02340"
     CDS             3557..4354
                     /gene="djlA"
                     /locus_tag="VFP93_02340"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HET9843482.1"
                     /translation="MFIFGKITGFFFGLLLGGMFGAFIGVLIGHYFDLQLKRTMQELG
                     GKFSATLEHTREEFFNITFEIMGYLSKIDGRVSEQEIHVARSIMNQLALDDAKRMDAM
                     DAFSRGKQSDFILEPRLIELKKILARAAHLKQLFLEIQLNIAYAEGFISTAKQEVIEK
                     ICHTLNVPLFLLRILETRFKAEQNFHHSANTTPKEDLNDAFGILGVSKESDHKTVKKA
                     YRKLMNEHHPDKLAAKGLPESMRPIAKEKTQKIQMAYETICKIKGWN"
     gene            4416..5093
                     /locus_tag="VFP93_02345"
     CDS             4416..5093
                     /locus_tag="VFP93_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9843483.1"
                     /translation="MKILKIIVILTLFYQSIVWASTPLGKPIRAVKTTEKIAFLSFDD
                     GPSVPYTQQILDLLDKYKVKATFFVVGINVKRYPQIIQDIMKRGHEIGNHSLSHENFK
                     NKSVDFVVQDLKKTDDLIKAQGYQKTIPFRAPFGILTKNLEVALRKLEKPHILFNFLP
                     KDWENPSPQVIHDRVIERASPGFIITLHDGWKNRKNTVIATEMIIKTLQQKGYRFVTA
                     TEYLKYN"
     gene            complement(5145..>5540)
                     /locus_tag="VFP93_02350"
     CDS             complement(5145..>5540)
                     /locus_tag="VFP93_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843484.1"
                     /translation="VLQKRAAEWRELFSQWELMPDIKQRLIALVHHMVSQAETLARFG
                     CMVGSLCQELGKQGGSIASGAAKLMSDIMAWSKSQFQSLGKHEDDSRLLAEQLIARIQ
                     GGSLLTLTFKEPAYMNRQTQATEEWLASL"
BASE COUNT         1764 a         1001 c         1054 g         1721 t
ORIGIN      
        1 ttaggataac gactttgatc gcgaaaaggc acatataaat aataaagcct aggctccatc
       61 gtttgctgat acgcattggg gtaaaattta tcaaaaaaca atccggtatc aatcgcgtag
      121 atcggaatcg cacgattcgc atgattcgga aaaaataatc tttcatgctc cggacttaaa
      181 tttaagtgat aacccacata atccattttt acactgggtt taaagtaacc gccaattcgc
      241 tccatcggta aactaagggc cggttttaaa taaaaacgct caccattcgt tacaatgtca
      301 tcggtaaatg gatcattttt gtgggtaaaa tgggtcgcct cgccttccaa attaaagcta
      361 aaaaaacgcg caaattttgg atagatgccc tgaaacgccc attgcggtaa tcttccatat
      421 tcttcgaaag taatcgggcc aagtaaagga tgtaaagaat cgatattttg caatcgccac
      481 cagtgatgcc aatgaatatt ttgataatgt aaattcacct cttgatttaa tttattggta
      541 cttgcgctat ttaagtcgtt ttctaaatca taaaaataat tatcatcact cacataagca
      601 taatcgatag ctaaattcca atattttgaa aaattggtgg cgtgttgaaa cctaatgcct
      661 aatctatcat taccatgctc taaaccttgt aagcgtggat ccaacagggg aatttctaat
      721 gggggtgata ataaattaat tgcccgaaat gctttatatt ttcgatctcc aggtaaaaaa
      781 tgtaattgaa aaataccaga gttaaaggat gaaagataac gtaattcatt ttgtaaatct
      841 aaaccgcgtt cggttaaaac gcggggggta aatgtggcat cataatttgg cgctatattc
      901 caatacagtg gccagctcaa ctcaaaaccc gattgtgtgg tagagcctac gttcgggtat
      961 aaaaatccgg tatgcctttt attatcaatg ggaaattgta aatacggcca ataaaaaata
     1021 ggtatgcttt ttaagtgcaa agaaatatgg cgtgcctgac ccattcctgt atcacgattt
     1081 aattgaatgt gtgttccttg aagcttccag gttggatcat tcggaggaca tgtcgtataa
     1141 cttgcacctt ccaaaagtaa tttacgttgt tcttcaatcg ttactttacg cgcactgcca
     1201 tgcgcatgac gtggaaaata aaaataatgt ggattgtata caacgattat atcgttttct
     1261 gcttgccaaa agcctgaaga accttgaatt tttaaatcgg ggtcttggta aataatcgaa
     1321 gaattcgcat gaatttcttt taaaaccttt tcaacatgga atatttcaag ctcttttgca
     1381 aaaaaatgac gttgtccata aaccaactga acactgcctc ttaaagtgga ggttccttct
     1441 cggatcatgg tcaattcatc tgcgctaatt tcccatttag gatcttctct caatacgtcc
     1501 catactggaa catggtaaaa ccctttgcat aaaagcgatg gatcctcgac ccaaccgtat
     1561 tgatttgaat ctaggggttc tgcaaacacc tgctcggtag gattaaattc ttgaaagtca
     1621 gcttgctcgg caagtgctga ttgtaggtta aatcccgtca ctaaaaatat acaaagagtt
     1681 gttttgcgaa taatgcgcat tccaagtagt ctaccttaga gcactatgcc aatatgagag
     1741 tcagatgtac aattggttgc caggataata tggtgtcgat gttctcacac agtagactgg
     1801 ctgtaaagct aaaaagaata ggccaaggtt taagatatga cagatcgcat gcatgcatta
     1861 acacattggg tttcaagtca gtttgcatca agaactgaat taataccgct tcataatgat
     1921 gcgagtttca gacgttattt tcgttttttt catcaaaata tttcttatat tgtagtggat
     1981 gcgccaccca caaaagaaaa taatgaagcg tttattttta tgacgcaact ccttgcgcaa
     2041 caaggtatta cggttcctaa agtgtataat ttcgatttgc agcaaggatt tattttactt
     2101 tccgattttg gcgacacact tttattagat attcttaatg ccgaaaatgc ggatcaatgg
     2161 tatagcaact cctttgaaat tttgcataaa ttgtctacat tccctaaaga ttttcatcct
     2221 aaaattccat tatttaatcg tcagcatatt ttgttagaac taagctattt cacagaatgg
     2281 tttctaacta aactattaaa cgtaacctta aatgtgcatg ataaagcatg tttatatcaa
     2341 gttttcgaat tgcttattaa tgcatgcgaa acccagccac aaattataat acatcgtgat
     2401 tttcattctc gtaacttaat ggtgctatca acgggtatgc tgggaactat cgattatcag
     2461 gatgctatgc ttggtcctct tacctacgat ttgtcatctt tgctaaaaga ttgttacgtc
     2521 acttgggatt cttaccgcgt ccaagcttgg gtcaagcatt attatcagat tttatgcggt
     2581 aaaatggcaa tcaaacattt ctctttcgaa cgttttcttc tgtgttttga ttgggtaggt
     2641 ttgcaaagac acttaaaagt attaggaatt ttctctcgtc taaaattacg cgacaacaaa
     2701 gcgcgctatt tagcggatat accccgtgtt ttacactatg tgctaaatac tgcacgcgaa
     2761 taccctgaat ttgaaaaatt ttattatttt atgcaggaca aagtcactcc ccgatttgaa
     2821 gcctttttgc aacaaacggg aaaacaaaat atagcataag gaattttatg cgtgccatga
     2881 ttttagcagc aggtaggggc agccgattaa agccgctcac agatacgtgt ccaaagcctt
     2941 tggttcctgt ggcaaataaa cctttaattg tttaccactt ggaaacactt tataccatgg
     3001 gggttcgtga agttgttatc aatgtttctt atttaagaga aaagattata cagttcttag
     3061 ggaatggtaa tacttttggc ttaacgatag aatattctat cgaagaatac gctttagaaa
     3121 cgggaggcgg tatttataac gctttgcccc ttttaggaag taatcctttt ttactagtaa
     3181 gtagtgatat atttatggat tattcaggcg ttaattttcc tatcttagat gttaaacttg
     3241 cccatctttt tttggtgcct aatccttcac acaatgcaaa gggggatttt ggattaaaac
     3301 aaggtgaagt tgtaagaaaa cgatcgcaag ataaaagtta tacatacgca aacttagctc
     3361 tattaaagcc tgcgttattc caatattgtc aatccgaagc tttcccatta gttgctgtta
     3421 tattacaagc aataatgcat caaggagtat ctggtgaatt gtttcaaggt cattggtcag
     3481 atgtaggaac gctggaaagt ttgcaaaaat tagaagataa cataaaccaa caatttgttt
     3541 gcgcgtagta aaattcatgt ttatttttgg aaaaatcacc ggctttttct ttggcttact
     3601 cttaggtggc atgtttggtg cttttattgg tgttctcata ggacactatt ttgatttgca
     3661 attaaagaga accatgcaag aattaggtgg gaaattttct gccactttgg agcatacccg
     3721 agaagaattt tttaatataa catttgaaat catggggtat ctttccaaaa ttgatggaag
     3781 agttagcgaa caagagatcc acgtggctcg aagtataatg aatcagttag cactggacga
     3841 tgcaaaacgc atggatgcga tggatgcgtt ttctcgcggt aaacaaagtg attttatttt
     3901 agagccacgc ttaatcgaac ttaaaaaaat tttggcacgt gcagcgcact taaaacaatt
     3961 atttctggaa atacaattaa atatcgctta cgcagaaggg ttcatttcta cagcaaagca
     4021 agaggtgatt gagaagattt gccacacgct taatgtgcca ttatttttat taagaatatt
     4081 agaaacgcga tttaaagcgg agcaaaactt tcaccactct gcaaatacta cgcctaaaga
     4141 ggatttaaat gatgcttttg gaattttagg ggtatctaag gaaagtgatc ataaaaccgt
     4201 taaaaaagcc tatcgcaaat taatgaatga acaccatcct gataagttag cagctaaggg
     4261 tttgcccgaa tccatgcgac ctattgccaa agaaaaaacc caaaaaattc aaatggcgta
     4321 cgagactatc tgtaaaataa aaggctggaa ttagatcaag attcttaagc ctagactctt
     4381 cactataatt cttatacaga agtaaggagt taaagatgaa gatactgaaa atcattgtta
     4441 ttttgacatt attttatcaa agtattgtgt gggctagtac gccgttagga aaacctatcc
     4501 gcgctgtaaa aactaccgaa aagattgcct tcttatcgtt tgatgatggt cccagtgttc
     4561 cttacaccca acaaatttta gatttattag ataagtacaa agtaaaagct actttttttg
     4621 tggtgggtat taatgttaaa agatacccgc aaattatcca ggacattatg aagcgtggtc
     4681 acgaaatagg taaccattct ttgagtcatg aaaattttaa aaataaatct gtagattttg
     4741 tggtacaaga tttgaaaaaa accgatgatt taatcaaagc gcaaggttat caaaagacga
     4801 taccttttcg tgctcccttt gggatattga ccaaaaactt agaagttgct ttacgtaaat
     4861 tagaaaagcc gcatatatta tttaattttt tacctaaaga ttgggaaaat ccctccccgc
     4921 aagtgattca cgacagagta attgaacgcg caagccctgg gtttattatt acgctacacg
     4981 atggctggaa gaatcgcaaa aatacggtga tcgctacaga aatgataatc aaaacgctgc
     5041 aacaaaaggg atatcgtttt gttactgcaa cagaatattt aaaatataat tagcaaaatt
     5101 gaaaattact ttgtacgaga agggcggtat tccgccctat gcatttacaa agaagccaac
     5161 cattcttcag ttgcttgagt ctgtctattc atataagcgg gttctttgaa agtaagggtg
     5221 agtaaacttc caccctgaat acgcgcgatg agttgttctg ccaagagtct agaatcgtct
     5281 tcgtgctttc ctaaggattg gaattgtgat ttagaccagg ccataatgtc agacattaat
     5341 tttgcagcac ccgatgcaat agagccacct tgtttgccta gttcttgaca cagagatccc
     5401 accatacagc caaaacgcgc taatgtttct gcttggctaa ccatatggtg cactaaagca
     5461 attagacgtt gtttgatatc gggcattaat tcccattggc taaatagttc acgccattct
     5521 gcagcacgtt tctgtaacac
//
LOCUS       DASTIB010000137         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_239003, whole genome shotgun sequence.
ACCESSION   DASTIB010000137 DASTIB010000000
VERSION     DASTIB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239003"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFP93_02355"
     CDS             <1..598
                     /locus_tag="VFP93_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET9843485.1"
                     /translation="AQGLSGPLQVQINQGIIEGLDLEYYLESARALLKKEQRPKNDRK
                     RTSFTTLKGTFLANHNYLTNNDLRLDADNLYALGDGSIDLNQKYLEYKFLVHKIYHDG
                     KQHPNALPLAVRVKGPFNDIKIMPDIDAYLKTVVKKEAQEELEEQLNKHLDKILRKEK
                     TPESNGSPPPTEDNASPESVEDKIEEEIDKGLKKLFKF"
     gene            598..1680
                     /gene="mutY"
                     /locus_tag="VFP93_02360"
     CDS             598..1680
                     /gene="mutY"
                     /locus_tag="VFP93_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9843486.1"
                     /translation="MQLTNTNRTWFAKEVIYFAKNFGRFHLPWKNPATPYRVWVSEVM
                     LQQTQVQSVLGYFDRFMDRFATIESLAQSPCDAVLSLWSGLGYYRRAHHLHQAAQIIT
                     QELNGRFPNDLETLVKLPGIGRSTAGAILAQGFNLPAPILDANVKRLLMRFFCIEGDK
                     AQTSILNQLWELSTQLTPKREVAIYTQGIMDLGAMTCTKSQPKCDECPLQLKCLSFRY
                     NKQHSIPEKAPKTIKPKRFCLFLVHLNAQREVYMLKRPNHGIWSGLWCFPILPFTESV
                     NKLNKLLKCSKYKLGNEAKHTFTHFDLVYKPVMIQNQNLVKCKNKHYRWLKLEQLADF
                     GIPSAVKKILQGQDFEQFKNDLLSQA"
     gene            1658..1921
                     /locus_tag="VFP93_02365"
     CDS             1658..1921
                     /locus_tag="VFP93_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET9843487.1"
                     /translation="MIYCHKLKKEAPALEKPPLPGILGQEIFAKISQEAWRMWLMEQT
                     MLINEMRLSLLDPDARTFLQNQMQKFLFEDTYTRPTGYIPPKS"
     gene            1972..2047
                     /locus_tag="VFP93_02370"
     tRNA            1972..2047
                     /locus_tag="VFP93_02370"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2005..2007,aa:Phe,seq:gaa)
BASE COUNT          722 a          429 c          403 g          605 t
ORIGIN      
        1 tgctcaaggt ttaagtggtc cccttcaagt acagattaat caaggcatta ttgaaggtct
       61 cgatttagaa tactacttag aaagcgcacg cgccttatta aaaaaagaac aaagacctaa
      121 aaacgatcgc aaacgtacca gttttactac attgaagggg accttcttgg ccaaccataa
      181 ttatttaacg aataatgatt tgcgccttga tgcggataat ttatatgctc taggggacgg
      241 ctccatagat ttaaatcaga aatacttaga atataaattc ttagtgcata agatttatca
      301 tgatggtaaa caacatccca atgcgttgcc ccttgcagtt cgcgttaaag gtccttttaa
      361 tgatattaaa attatgccgg atatcgatgc ctatttgaaa acggtagtca aaaaagaagc
      421 ccaagaagaa ttagaagaac aattaaataa acatttggat aaaatattac gcaaagaaaa
      481 aacacctgaa tccaacggat cacctccacc gactgaagac aacgcaagcc ctgagtcggt
      541 ggaggataaa atcgaagaag aaattgataa aggtttaaag aagttattta aattctaatg
      601 cagttaacaa atacaaatcg cacttggttc gcaaaagaag ttatctattt tgctaaaaat
      661 ttcggaagat ttcatttacc ctggaaaaac cctgccaccc cttacagggt ttgggtctcc
      721 gaagttatgc ttcaacaaac acaagtacaa agcgtattag ggtatttcga tcgtttcatg
      781 gatcgatttg caactattga atccctggca caaagcccgt gtgatgcggt tttatcgctt
      841 tggtcgggac ttggctatta tcgaagagca caccatttac atcaagcagc gcaaataatc
      901 acccaagaat tgaacggacg ttttccgaac gacttagaaa ctttagttaa attaccagga
      961 atagggcgtt ccaccgccgg agccatcttg gcgcaaggat ttaatctccc ggcacccatt
     1021 ctggacgcaa atgtaaaaag attattaatg cgattttttt gtatagaagg tgacaaagca
     1081 cagacttcga ttctgaatca attatgggaa ttaagtacac aattgacgcc taagcgagaa
     1141 gtagcgattt atacccaggg tattatggat ttaggggcaa tgacttgcac taaatcacaa
     1201 ccaaaatgtg atgaatgtcc tttacagtta aaatgtctat cttttcgcta taacaaacaa
     1261 catagtattc ccgaaaaagc acccaaaaca attaagccca aacgtttttg cttattcctc
     1321 gtccatctta acgctcagcg cgaagtttat atgctgaagc gcccaaatca tggcatttgg
     1381 tctgggttat ggtgctttcc tattttaccc tttaccgaaa gtgtaaataa attaaataag
     1441 ctcctaaaat gctcaaaata taaattgggt aacgaagcca aacacacttt tacgcatttt
     1501 gatttagtat acaaacctgt tatgatccaa aatcaaaatt tggtaaagtg caagaataaa
     1561 cactatcgtt ggttaaaact cgagcaactg gcggattttg gcataccttc cgctgttaaa
     1621 aaaattttac aaggacaaga ttttgagcag tttaaaaatg atttattgtc acaagcttaa
     1681 aaaagaagcc ccagcattag aaaaaccacc cctccccgga atattaggcc aagagatttt
     1741 tgcgaagatt agccaagaag cctggcgcat gtggcttatg gaacaaacta tgctcattaa
     1801 tgaaatgcga ctaagtttgc tggatccgga tgcgcgcaca tttttgcaaa accaaatgca
     1861 aaaattttta tttgaagata cttatacgag gcctacaggg tatattccac caaagtcgta
     1921 gttaaagctt gactcttagg gccttttcga gtttaattcc ccaatcttaa tggccagata
     1981 gctcagttgg tagagcaaag gactgaaaat ccttgtgtcc ctggttcgat tccaggtctg
     2041 gccaccatcg actagattac gcagccttct ccagttcaaa aagtaatgct aataaataac
     2101 tctctttttc ttttggtaat ctattctcag ctgaacaaga agtagttttt tcccatcga
//
LOCUS       DASTIB010000138         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1113466, whole genome shotgun sequence.
ACCESSION   DASTIB010000138 DASTIB010000000
VERSION     DASTIB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113466"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1986
                     /gene="typA"
                     /locus_tag="VFP93_02375"
     CDS             169..1986
                     /gene="typA"
                     /locus_tag="VFP93_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET9843488.1"
                     /translation="MIKNLKNIAIIAHVDHGKTTLIDKLLQQSGTLGRQQDVSCVMDS
                     NELEKERGITILSKNTAIQWGGFHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAVD
                     GPMPQTRFVTEKAFKQKLKPIVVVNKIDRPGARPDWVIDQVFDLFDKLGATDEQLDFP
                     VVYCSALLGTATLELEKPGQNLDPLFQTIIEKVPSPACQIEGPFQMQISSLDYSSFVG
                     TIGIGRIQRGTIKTNTPVAVIDKDGKTRTARILQVLGYLGLNRAEIPQAQAGDIVAIT
                     GISPLSISDTLCDPNCVEPLAPLLVDEPTMSVLFQVNKSPFAGLEGDFVTSRQIKDRL
                     EKEIVHNVALKVEATNDPDKFKVSGRGELHLGILMENMRREGYEFAVSRPEVILKETE
                     NGVEEPYELLTIDIEEAHQGSVMEMLGSRKGELKNMMSDGQGRTRLDYVIPARSLIGF
                     HTMFLTLTSGTGIATHIFSHYDTFKEGVEVQRNNGVLIANEAGKATAYSLYNLQPRGR
                     LFIEPQTEVYEGMIVGLHARSNDLVVNVTKGKKLTNMRASGSDENIILTPPVRHSLEQ
                     ALEFINDDELVEITPKSIRLRKKWLKEHERKRYSREEMS"
     gene            2023..>2190
                     /locus_tag="VFP93_02380"
     CDS             2023..>2190
                     /locus_tag="VFP93_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET9843489.1"
                     /translation="MKTLHPPIKPYAQHMLEVEAPHQIYVEECGNPNGLPILFVHGGP
                     GAGCTTDDRRFF"
BASE COUNT          703 a          394 c          486 g          607 t
ORIGIN      
        1 cgatagttct ttaattatta tcggaccaca ccaagaaaat ttgagttctg tctattacag
       61 tataggtcaa tatacgtgta cctgctaaat ctataaaaaa gagtaacttt cagtaagatt
      121 actggtagaa tattgaaggc atagtaaata attttttttg gggctgtagt gattaaaaat
      181 ttgaaaaata tcgctattat cgcgcacgtc gatcatggca aaaccacact cattgataaa
      241 ctattacaac aaagcggtac attaggtcga caacaggatg ttagctgtgt tatggattca
      301 aacgaattag aaaaagagcg cggaatcact attttatcca aaaataccgc aatccaatgg
      361 ggtggttttc atattaatat tgtagataca ccggggcacg ctgattttgg cggcgaggtt
      421 gagcgggtac tatctatggt agattcagtt ctattattgg tagatgccgt agacggaccg
      481 atgccacaaa cgcgcttcgt tacggaaaaa gcttttaagc aaaagctaaa acctattgtt
      541 gtagtcaata aaatcgatag acctggagca agacccgatt gggttatcga tcaagttttt
      601 gatttgttcg ataaattggg agctaccgat gagcaactag attttcctgt ggtctattgc
      661 tctgcacttt taggtactgc cactttggaa ctagaaaagc caggccagaa tttagatcca
      721 ttgtttcaaa ctattatcga aaaagtacct tctcctgctt gccaaatcga aggtccattc
      781 caaatgcaaa taagttcttt ggattattca agttttgtag gaaccattgg cataggcaga
      841 atacagcggg gcacgataaa aacgaatact ccagtcgctg tcatcgataa agacggaaaa
      901 acgcgtaccg cgagaatatt acaagtttta ggttacttag gcttaaatcg cgcggaaatt
      961 ccacaagcgc aagctggcga tattgtggca attacaggaa ttagcccact atcgatttcg
     1021 gacacgttgt gcgatccgaa ttgtgttgaa ccattagcgc cgttattggt agatgagccc
     1081 accatgagtg tgctttttca agtgaacaaa tcaccttttg caggtctaga gggggatttt
     1141 gtgacaagtc gacaaattaa agatcgactg gaaaaagaaa ttgtacacaa cgttgctctt
     1201 aaagtcgaag ctacgaatga tcccgataaa tttaaagttt cgggtcgggg cgagctccat
     1261 ctcggtatat taatggaaaa tatgcgcaga gaaggctatg aatttgcggt ttcacgtcca
     1321 gaagttatct taaaagaaac tgaaaatggc gttgaagaac cttatgagtt attaacgatc
     1381 gacattgaag aagcccatca aggtagcgtc atggaaatgt tgggatcacg caaaggtgaa
     1441 ttaaaaaaca tgatgtctga tggtcaagga cgaacccggt tagattatgt aattcctgct
     1501 cgcagtttaa taggttttca tactatgttt ttaacgctaa cttcgggcac aggcatagcg
     1561 acccatattt ttagccatta cgacaccttt aaagaagggg ttgaagttca aaggaataac
     1621 ggtgtattga tagccaatga agcgggtaaa gctacagcat attctttata taacttacaa
     1681 ccgcgcgggc gattgtttat agagccacaa acggaagtat atgaaggaat gatagttgga
     1741 ctgcatgcac gatcaaatga tttggtagtg aatgtaacta aaggaaaaaa actgaccaat
     1801 atgcgcgcgt cgggatccga tgaaaatatt attttaacgc ctccggttcg ccattctctc
     1861 gaacaagctt tagaatttat taatgacgac gaattggttg aaataacacc caaatctatt
     1921 cgtttaagaa aaaaatggtt gaaggaacac gaaagaaaac ggtattctcg tgaagagatg
     1981 agttaatttt aagagttaaa tttaaagtta aagggattta ccatgaaaac tttgcatccg
     2041 cccatcaaac cttatgcaca acatatgttg gaagtggagg cgccgcatca aatctatgta
     2101 gaagaatgtg gcaaccccaa tggtttaccc atattatttg tgcacggagg gccgggtgct
     2161 ggctgcacca ccgacgaccg tcgatttttt
//
LOCUS       DASTIB010000139         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1374770, whole genome shotgun sequence.
ACCESSION   DASTIB010000139 DASTIB010000000
VERSION     DASTIB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374770"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2332
                     /locus_tag="VFP93_02385"
     CDS             <1..2332
                     /locus_tag="VFP93_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9843490.1"
                     /translation="VKQEEGQLVRLKEVAKVTIEPATERFLARYNTNETVALGIVKQS
                     TANPLEIAQTVEALLPQLSANMPKGMHIEIAYDSTLTIKNSIESVFKTLFEAVILVIF
                     VIFVFLRSMRATLIPLITIPISLISAFAIMAAFNFSINTLTLLAMVLAIGLVVDDAIV
                     VLENIHRHVESGLSPLESARKGMKEIGFAVIAMTLTLLAVFAPIAFSRGYVGKLFTEF
                     AIVLSGSVVVSGVTALTLSPMMCSRLLKPENSHGRVYLWIERFLEGLSTLYARTLKVA
                     LNHTIWVLGIFIVVLALNYFLFTGLKSELAPPEDRGFIIAIGMAPQGSSVHYTNKYAK
                     QMEQVLANNPNIQRFFTIIGYPNVTQTMSFAMLPSHDKRDVSQFEVTEQLNKHFWGIS
                     GLASFAINPPSSIEGGGSRGDVAFVIQSPKGFEDLGTYAEIILNKARQNPKLLNLDID
                     FKLDKPQIELHVNRERAAMLGVDLSVIGQTLQVLYGGKKASTYSQGSQQYDVLVQADS
                     ATRQTPESIHSVYVRSKNNTMIPLASLVEPKETVAANALNRFNKLPAAVITAALSPDY
                     TLQEALSYFEALTQEVNSAFLIDYAGASRTFKQSVNTLAQAFILALLVIFLVLAAQFE
                     SFKSPFIIMLSVPCAIFGALLILSITEGSLNIFSQIGLITLIGLITKHGILLVEFTQQ
                     LRQQGLGLQESLLESARLRFRPILMTTAATVLGALPLAIATGAGAEGRNQIGWVIVGG
                     MLIGTLFTLFIVPAIYKIFSKKTVSVTSVDKQGDLA"
     gene            complement(2298..2762)
                     /locus_tag="VFP93_02390"
     CDS             complement(2298..2762)
                     /locus_tag="VFP93_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9843491.1"
                     /translation="MNQQSLQEQFAPENICFGCGPSNTQGLRIKSFVKGQQVIANWQA
                     EKYHEAFPGILNGGIIGTLLDCHANWTAAYFLMQSQQLDRPPCTVTSYFNVKLKKPTP
                     SDKAVHLIAQPLEIQNDRAKILAQLYSDGVLCAECEGLFVAVKPNHPAYQRW"
BASE COUNT          807 a          568 c          616 g          904 t
ORIGIN      
        1 agtaaagcaa gaagaaggtc agctagtccg tcttaaggaa gtggcaaaag ttactattga
       61 accggccacc gaacgatttt tagcgcgtta taataccaac gaaacggtgg cgctaggaat
      121 tgttaagcaa tctacagcaa atcctttaga aatcgcccaa accgtggaag ccctattgcc
      181 gcaattatcg gcgaatatgc ctaaaggtat gcatatagaa attgcgtatg attccaccct
      241 caccataaaa aactcgattg aatcggtttt taaaacgcta tttgaggcag tgattctggt
      301 catttttgtt atttttgtgt ttttacgatc catgcgcgcg accttgattc ctttaatcac
      361 cattcctatc agtcttatca gcgcctttgc tatcatggcc gcttttaatt ttagcattaa
      421 tacgttaaca ctattagcta tggtgttagc gataggttta gtggtagatg atgctattgt
      481 ggttctggag aatatccatc gacatgtgga atcagggtta tcacccttag aaagcgcccg
      541 taaaggcatg aaagaaatcg gttttgcggt gattgccatg acgcttacct tattagccgt
      601 gtttgctccc atagcgttta gccgagggta tgttggcaaa ctctttaccg aatttgctat
      661 tgttttatcc ggaagtgttg tggtgtctgg agttaccgca cttacgctat cacctatgat
      721 gtgttcgcgt ttacttaagc ccgaaaactc acatggaaga gtctatttat ggattgaacg
      781 ttttctggag ggtcttagta ctctttatgc acgtacctta aaagtggcac taaaccatac
      841 gatttgggtg ctaggtatct ttatcgttgt cttagcctta aattattttt tatttacggg
      901 cttaaaatcg gaattagccc ctcctgaaga tcgcgggttt attattgcca tagggatggc
      961 gccacaaggc tcttcggtac attatacaaa taagtacgca aaacaaatgg aacaggtatt
     1021 ggccaataat cccaatattc agcgtttttt taccattata ggctatccga atgttacgca
     1081 gaccatgtct tttgctatgc ttccttccca tgataaacgc gatgtttccc aattcgaagt
     1141 gaccgaacaa ttgaataaac acttttgggg tatttctggg ttggccagtt ttgcaatcaa
     1201 ccccccctct tccatcgaag gagggggttc acgaggggat gtagcctttg taattcaaag
     1261 tccgaaaggt tttgaagatc ttggtaccta tgcggagatt attttaaata aagcgcgaca
     1321 aaatcccaag cttttaaact tagacattga ttttaaatta gataaaccgc aaatagagtt
     1381 gcatgtcaat cgcgaaagag ccgcgatgct tggcgtggat ttaagtgtaa tcggtcaaac
     1441 gcttcaagtc ttatatggtg gtaagaaagc atccacctat tcgcaaggca gtcaacaata
     1501 tgatgttctc gtgcaggcag attcggcaac cagacaaaca cccgagagca ttcattcggt
     1561 ttatgtacgt tctaaaaata atactatgat ccccttggca agtttggtag agccgaagga
     1621 aaccgtagcc gccaatgcgt taaatcgttt taacaaatta ccggctgcgg ttataacggc
     1681 agctttaagt ccggattata cgttgcaaga ggcgttgagc tattttgaag cgcttaccca
     1741 agaagtaaac agtgcgtttc tgattgatta tgccggtgcc tctaggacat ttaagcaaag
     1801 tgtaaatact ctagcgcaag cttttatttt agcgctgtta gtgatctttt tagtattagc
     1861 ggcacaattt gagagcttta aatcaccttt cattattatg cttagtgtac cctgtgctat
     1921 ttttggtgcg ctgttaatac tatctatcac cgagggaagc ttaaatattt tctcgcaaat
     1981 tggtcttatc acgttgattg gtttaatcac gaaacatgga attttgttag tggaatttac
     2041 acagcaatta cgccagcaag gtttaggact gcaagaatcg cttctagaat cggctaggct
     2101 acgctttagg cccattttaa tgaccacggc agccaccgtt ttgggagctt taccgcttgc
     2161 catagcaaca ggtgcgggag cggaaggacg taatcaaatc ggttgggtaa ttgtcggtgg
     2221 catgttaatt ggcaccttat tcactctttt tattgttccc gccatttata aaattttcag
     2281 taaaaaaacc gtatccgtta ccagcgttga taagcagggt gatttggctt aaccgcaaca
     2341 aataatcctt cacattcggc gcacaaaaca ccgtccgaat acagttgggc caaaattttt
     2401 gccctatcgt tttgaatttc aagcggctgg gcaattaagt gcacagcttt gtcactcgga
     2461 gttggttttt ttagtttgac attaaaataa gaagttaccg tacagggagg acgatctaat
     2521 tgttgggatt gcattaaaaa ataggcggca gtccaattgg catgacaatc taataatgtg
     2581 ccaataatcc ccccgtttaa tattcctgga aaagcttcgt gatacttttc tgcttgccag
     2641 ttcgcaataa cttgctggcc ttttacaaaa cttttaatgc gtaaaccttg tgtattggaa
     2701 ggtccacagc caaaacaaat attttcgggt gcgaattgtt cttgtaaact ttgttgattc
     2761 atttattttt aagagttgat tcactgcaaa ataaataaaa attaaaaaaa ttacgtaagg
     2821 tacttgcgaa tttaaatgaa tttgctatat tccaaatgcc agtgggcatt ctttgtttga
     2881 tgtgtgcaat aagct
//
LOCUS       DASTIB010000140         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_501660, whole genome shotgun sequence.
ACCESSION   DASTIB010000140 DASTIB010000000
VERSION     DASTIB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501660"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFP93_02395"
     CDS             <1..249
                     /locus_tag="VFP93_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843492.1"
                     /translation="IPQPLFKQFLAQLLEKKQIKYGMEPANAKEFAAKNLENILNDLL
                     DQGYIKLNATHYDIDALFTHGSLKMFGVPKAFPKYLLE"
     gene            complement(238..627)
                     /locus_tag="VFP93_02400"
     CDS             complement(238..627)
                     /locus_tag="VFP93_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET9843493.1"
                     /translation="MTLGRFVLILFFIAFAFITSHLTLSFVHKQDIHQLSSGEKKCVH
                     CNLNKADLAKWDLRGYDLTQASMVGADLRCANLEKAILAGATMKFANLTGANLEGADL
                     LFTNLEQVVWEDGTDCEELADPAPLLQ"
     gene            complement(692..1711)
                     /locus_tag="VFP93_02405"
     CDS             complement(692..1711)
                     /locus_tag="VFP93_02405"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9843494.1"
                     /translation="MDPLFIKNLPKAELHVHIEGTLEPELLLKLAEKNKIQLPFTSIE
                     AVKQQYQFENLQDFLDLYYMGTQVLQTAEDFFTLTAEYLTRAHQNGITHCEIFFDPQS
                     HTQRGIPFAAVFEGIYQALQEGEKKYQLSSHLILCFLRHLSQAHAFATLKEANPFKPW
                     IIGVGLDSSEKNNPPRKFQEVFKSARQAGYKVVAHAGEEGPADYIWQSITLLKAERID
                     HGVRCLEDPQLVRFLADNRIPLTVCPLSNVKLKIFPTLCQHNLPQLLEQQLCISIHSD
                     DPAYFGGYINDNFQAVAQTFSLSNAQLIGFAKNSFLASFLCDTEKKQALASIDSFMRM
                     TTLTG"
     gene            complement(1909..>3336)
                     /gene="mutS"
                     /locus_tag="VFP93_02410"
     CDS             complement(1909..>3336)
                     /gene="mutS"
                     /locus_tag="VFP93_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9843495.1"
                     /translation="IPRLIEYCKLPEQTVFATRVKKIQTFPNIIALLDKAMEDNPPMT
                     VRDGGVIRFGYDAQLDELRLIYQDCEEYIKKIEQQEQKKTGISTLKVDYNKVHGFYIE
                     ISKGQAKNAPVGYQRRQTLKNAERFITEELKKLEDKVLSAKEKSLALEKKLYQDILAH
                     LVDYVKPLQNTATYLAQIDVLLNLAERAETLNLVKPHLVDAHTLHIQGGRHPVIAHAT
                     CESFVPNDLHLDDATKLLLITGPNMGGKSTYMRQTALIVLLAHIGSFVPATSAILGPI
                     DRIFTRIGAHDDLASNQSTFMVEMTETAQILHHASHKSLVLMDEIGRGTSTYDGLSLA
                     WAVLEYLSTKVQAYTLFSTHYFELTQLAENLPNTQNCHLDAKEHERGISFFYKVEPGP
                     ANKSYGIQVAKLSGIPLEVIQKAQEKLCTLETASTSQPKETQRFEQKSPTWLNLVAQI
                     QPDHCSPKEALEFLYQLKKAFQEEH"
BASE COUNT          963 a          633 c          662 g         1078 t
ORIGIN      
        1 ataccacaac ctctgtttaa gcaattttta gcacagttat tagagaaaaa gcagattaaa
       61 tatgggatgg aaccggctaa cgcaaaagag tttgccgcta agaatttaga aaatatctta
      121 aatgatttat tggatcaggg atatattaag ctcaacgcca cacactacga tatcgatgcg
      181 cttttcacgc atggctccct aaaaatgttt ggcgtaccta aagcttttcc aaaatattta
      241 ttggagtaat ggagcaggat ccgcaagctc ttcgcaatcg gtaccatcct cccaaacaac
      301 ctgttcaaga ttggtgaata ataagtcggc gccctccaag ttggcgccgg ttaaattggc
      361 aaatttcatt gttgcacccg caagaattgc tttttctaag tttgcgcaac gtagatcagc
      421 acctaccatc gatgcctggg taagatcgta gccacgcaag tcccatttcg ctaaatccgc
      481 tttattcaaa ttgcaatgta cacacttttt ttcgccagaa ctaagttgat ggatatcctg
      541 tttatgcaca aaggaaagcg ttagatgcga ggtaataaac gcaaatgcaa taaaaaataa
      601 aattaaaaca aatcgtccga gtgtcatata aacgcttgta ggactttact attaattatt
      661 gctgaaaacg tgctacggtg agacaggaaa tttatccagt taaagttgtc attcgcataa
      721 agctatcaat gcttgctagc gcttgctttt tttccgtatc gcaaagaaaa cttgctagaa
      781 agctattttt cgcaaaccct ataagctggg cgttggataa tgaaaaggtt tgggcaactg
      841 cttgaaaatt atcgttaata tagccaccaa aataggctgg atcgtctgaa tgtatggata
      901 tgcataattg ctgctctaac aattggggca aattgtgttg acataaagtt ggaaaaattt
      961 ttagcttcac gttggaaaga ggacatacag taagcgggat cctattgtcg gctaaaaaac
     1021 gaactaactg tggatcttcc aagcagcgca ctccatgatc aatcctttct gccttgagga
     1081 gggtgatact ctgccaaata taatccgcag ggccttcttc gcctgcatgc gccactactt
     1141 tatagcctgc ttgacgcgca cttttaaaca cttcttgaaa tttacgaggg ggattatttt
     1201 tttctgaaga atccagacct acgccaataa tccatggttt aaaaggattt gcttctttta
     1261 aggtggcaaa tgcgtgcgct tgagataaat gccttaaaaa acatagaatt aaatgtgaag
     1321 aaagttgata ttttttttca ccttcttgca gagcttgata aattccttca aaaacggctg
     1381 caaatggaat acctctttgg gtatgcgatt gtggatcaaa gaagatttca caatgggtaa
     1441 tgccattttg gtgcgcgcgc gttaaatatt ctgcggttaa ggtaaaaaaa tcttctgcgg
     1501 tttgtaaaac ttgagtaccc atatagtaaa gatctaaaaa atcttgtaaa ttttcaaatt
     1561 ggtattgctg cttaacagct tcaatcgagg taaagggcag ttgtatttta tttttctcag
     1621 ctaatttcaa cagaagttca ggctctaatg taccttcaat atgtacatgg agttccgctt
     1681 ttggaaggtt ttttataaag agaggatcca tacatttcct taagatcatc tcttattatt
     1741 ttagtgcatt cacatgcgtt gtaaaatacg cactctgtag gcataattac attccaagtt
     1801 tgataaccta attttttggt caaatctaca gtttttctaa tgatttctta ccataacata
     1861 ccttggtcgc ccaaggccaa gtcctataag ctatttagga cttaatactt aatgctcttc
     1921 ttgaaaagct ttttttaatt gatataaaaa ctcaagtgct tctttgggtg agcaatgatc
     1981 cggttgaatt tgcgccacca aatttaacca cgtaggagat ttttgttcga atcgttgcgt
     2041 ttctttgggt tgcgacgtgg atgctgtttc taaagtacaa agtttttctt gtgctttttg
     2101 aatgacttct aaggggatcc cgcttaattt tgctacttga ataccgtaac ttttgtttgc
     2161 tgggccaggc tctactttat aaaaaaaact gataccacgt tcgtgttctt tcgcatctaa
     2221 atgacaattt tgggtattag gtaaattttc cgccaattgg gttaattcaa aataatgtgt
     2281 tgagaacagc gtataagctt gcactttcgt acttaaatat tctaaaaccg cccatgcaag
     2341 cgataaacca tcgtaagtac tggtgccacg accaatctca tccatcagta ctaaactttt
     2401 gtgcgaggca tgatgtaaaa tttgtgctgt ttcggtcatt tccaccataa aagtggattg
     2461 gttggatgcc aaatcatcgt gcgcaccaat tcgggtaaaa atgcgatcga tagggcctag
     2521 gatggcagag gttgcgggaa caaagcttcc gatgtgcgcc agtagtacaa taagcgcagt
     2581 ttggcgcata taggtcgatt tgccccccat attgggccct gtaattaaca ataatttggt
     2641 ggcatcatct agatgaagat cattaggaac aaaagattca caggttgcat gcgcaataac
     2701 gggatgtctt cctccttgaa tgtgcagtgt gtgggcatcg actaaatggg gttttaccaa
     2761 gttcagcgtt tccgcacgtt cggctaaatt tagcaagaca tctatttgtg ccaaataggt
     2821 ggcggtattt tgtagaggtt ttacatagtc tactagatgt gcgaggatgt cttgataaag
     2881 ttttttctct aacgctaaag atttttcttt tgcacttaat actttatctt ctaatttttt
     2941 taattcttcg gtaataaaac gttctgcgtt ttttaacgtt tgtcggcgct ggtaacctac
     3001 gggtgcattt ttggcttgtc ctttgcttat ttcaatataa aaaccgtgca ctttgttata
     3061 atccactttt aaggtgctaa taccggtttt cttttgttct tgttgctcaa tttttttaat
     3121 atattcttcg caatcttggt aaatcaagcg cagctcatct aattgcgcat cgtacccaaa
     3181 acgtataaca cccccatcgc gcacagtcat cggaggattg tcttccatgg ctttatccag
     3241 taacgcaata atattaggaa aagtttgaat ttttttaaca cgcgttgcaa aaacggtttg
     3301 ttcgggtaat ttacaatatt cgataagtct tggaat
//
LOCUS       DASTIB010000141         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_626993, whole genome shotgun sequence.
ACCESSION   DASTIB010000141 DASTIB010000000
VERSION     DASTIB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626993"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VFP93_02415"
     CDS             complement(<1..1399)
                     /locus_tag="VFP93_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843496.1"
                     /translation="MLESEKTLAPKLNLMLCKDKSIPAPCGDNNIIFLFIRNMYDGVG
                     DFVHGVTFSKELNVLKKKGYKICALVSTMTEDPLNENCKFTKRANFVKDMLISGKHDF
                     DQWYLMEDLMNKAFDLKEWMTLSQQSDLIGNLNKAAAAFEISYGFNHQELWEHLPTEV
                     KVLKCRQYGSKAPNANPHALDLLTNASMGLSQENCEGLGIKLYSPQIDEKNRSQRLLA
                     ISQRNPDFVNQLLCTQHPSIEQAEHYFSSHHFAPAYLQNSPGATAFILTQILKYLHSE
                     GNLEKKCDFLIPANVVDEETLRFFLHGYGFKDSDVAFVTANSNIQDHQNAKIRIISFR
                     IHDDEDYDALFSMTQDGTGCSGDNSTSVCFSNRSLPYFQDKGAGSPLGNFYKTQLIFT
                     LTQEIAQCSDTMLKEGLHNLKNYFEHLIINDSPLLNGLSPIMQIEEGSLSNQNIVEYL
                     TKMYPTEFMRWIEKAA"
BASE COUNT          741 a          409 c          451 g          789 t
ORIGIN      
        1 aagctgcttt ttcaatccaa cgcataaatt cagtggggta catcttagtc aaatattcga
       61 cgatattttg attgctaagg ctaccctctt caatttgcat tataggagat aatccgttaa
      121 gcaggggaga gtcattaatg ataagatgtt caaaatagtt tttaagattg tgtaatcctt
      181 ccttaagcat ggtatccgag cattgtgcta tttcctgcgt taaagtaaaa atcaattgag
      241 ttttatagaa attaccgagc ggactacctg cacccttatc ttgaaagtaa ggaagagaac
      301 ggtttgaaaa gcaaaccgaa gtcgaattat cccctgaaca gcctgtcccg tcttgtgtca
      361 tgctaaacaa tgcgtcataa tcctcatcgt catgaatgcg aaaagatata attctaattt
      421 ttgcattttg atgatcttga atgttcgaat tagccgttac gaatgccaca tcggaatctt
      481 taaacccgta gccgtgaaga aaaaaacgca atgtttcttc atccacgaca tttgcgggaa
      541 ttaaaaaatc gcattttttc tcaagatttc cttcactatg aagatatttt aagatttgag
      601 ttaaaataaa ggcagtcgct cctggactat tttgaagata agctggggca aaatgatggg
      661 acgaaaaata atgttccgct tgttcgattg atggatgttg tgtgcacaga agctggttaa
      721 cgaaatcagg attacgttgg ctgatggcta acaacctttg cgagcggttt ttttcatcta
      781 tctgaggaga atataatttt atacctaacc cctcacaatt ttcttgggat aaacccatgg
      841 aagcattggt aagaagatcg agcgcatgag ggttggcatt tggtgcctta gatccgtatt
      901 gacggcattt taacactttg acctctgtgg gaagatgttc ccagagctct tggtggttaa
      961 aaccatatga aatttcaaat gctgcagccg ccttgttcaa attgcctata agatcgcttt
     1021 gctgtgataa cgtcatccat tcttttaaat caaaagcttt attcatcaaa tcttccatta
     1081 aataccattg atcgaaatcg tgtttgcctg aaattagcat atctttaacg aaatttgcgc
     1141 gtttggtaaa cttgcaattc tcattaagtg gatcttccgt catcgttgat accagggcgc
     1201 agattttata cccctttttc ttgagtacat tgagttcctt gctgaaagta actccgtgaa
     1261 caaaatcacc tactccatca tacatattgc ggatgaataa aaagataata ttattgtcac
     1321 cacaaggagc cgggatggat ttatctttgc acaacattag atttaatttg ggggctaaag
     1381 ttttttctga ttcaagcaat gagggggatg caaaatttca tacattattt acatagttat
     1441 acagctgaga taagttacat aataatgaaa ttgctgtaga actctaggtc acaataacaa
     1501 gatttcgatt aacgtcattg atagctagat taaaggtaaa cttacgcaaa tgttcaacag
     1561 agttttcatt tgtcgcgcta cctatttagg gcagttttgc ctaatgctac attttctagt
     1621 tcacaggtga attctgtgat aaaacaactg ttagagcgca tttgtttact atgcgcaagt
     1681 gtaaagccta ccccatttat gctgcctctt actgttattt tgttaacacg gatagattgt
     1741 gtaccattat aaaaatggaa tttattggga aaaagagagt caaatatcag agtacacttt
     1801 tatctttttg aaaaaaatca atggtgatag cttgtccttc aaaatttatt tcttttttga
     1861 aagaaaaatc ggttaagtca gtgctgatcg gaaatggata ttgtttgtca caccctgaat
     1921 tgggcaacgc atttaaatgg agtattaagg cattagaaaa attacataat tatttaccca
     1981 atgaagaaac aggctgccca gaaaggtatt taagttgtat caggcaaaat ttagtaaaag
     2041 acatggttca atattatgtt cgaaatattt gtaagacctc cgtttcttta ttttgagttg
     2101 ccaaaaaacg tacacgtagg tttgatgggt tcagtcaggt aatctttttg aagagactgg
     2161 gagctgttca tcggaaaatt tcatcattgc atcccttcct tttttgccaa tccaacataa
     2221 tatatataat tgaacctcac aacttttgag ttctatttat aatgatgtgg ctttattcat
     2281 ttataagggg tgataaaaaa tcatcagctc ctgaaacgca atatgtcggt ttttttagat
     2341 tgcttcgtga aactgttata aatcccgaga aaaaaattga ttttttttta
//
LOCUS       DASTIB010000142         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_677402, whole genome shotgun sequence.
ACCESSION   DASTIB010000142 DASTIB010000000
VERSION     DASTIB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677402"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..893)
                     /locus_tag="VFP93_02420"
     CDS             complement(57..893)
                     /locus_tag="VFP93_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843497.1"
                     /translation="MLKQLRSFLSMQQALKRVNIALTRAAAVAPLREVNLTNPHSWEF
                     SGFSQNGEDGILEQLIAKLKKPNKFFLEIGASDGLENNTSWLAIAKKYNGLMIEGNTK
                     LSNFCSKLMEKFNLGVECLPQFVHENEVQFILNKLPFLDPDIFSLDIDSYDYHVLKSL
                     FSKNFRPKICVVEYNSAFGPNLSITIPKNCVPNVNNYYYGVSINAWRHLFNAHGYSFI
                     TVESRGINGIFVDKNCYDAIFLNSLNSCAFQENFYQYTISKGDWRNQYEKIRNLPYED
                     VT"
     gene            complement(887..2005)
                     /locus_tag="VFP93_02425"
     CDS             complement(887..2005)
                     /locus_tag="VFP93_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET9843498.1"
                     /translation="MIPVNTPILGEQEKINLMQCIESGWISSEGPLVAEFEQQFASKV
                     GRKYGVAVMNGTAALYVAVRALKLPPGSEVILPTFCIISCLQAVIENQLVPVLVDCDE
                     HTWNMDCNVIEHKISKKTKAIMVVHTYGLPVDMEPLIQLANQYHLKIIEDAAEVIGQT
                     YKSKTCGSFGDISIVSFYPNKHITTGEGGMVLTDSKALAEQAKKLRNLAFEKDKRFYH
                     TELGWNFRMTNVQAAIGLAQLERLDDNIAKKREIGIRYAEAFKDIPYIQIPLAETTSA
                     QNIYWVFGIVLAKPLMRHHIIQQLEELGIGTRPFFWCMHEQPILQKMGYFKEEKHPVS
                     ENLARQGFYLPSGLGLSEEEQNYVIKSFKNIMEHRYPC"
     gene            complement(2009..2437)
                     /locus_tag="VFP93_02430"
     CDS             complement(2009..2437)
                     /locus_tag="VFP93_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843499.1"
                     /translation="MIEQIIFEDIVYAIIIRSEYKQEGITFVTPNDYSQQLAFMSHPK
                     GKKIQPHIHNKVMRSVTLTQEVLFIKKGKVRVDFYNPKTDYLGSVILEEGSVILLAYG
                     GHGFEMLEPTEMFEIKQGPFTGENDKTRFVCEEHAILIKE"
     gene            complement(2566..>3306)
                     /locus_tag="VFP93_02435"
     CDS             complement(2566..>3306)
                     /locus_tag="VFP93_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HET9843500.1"
                     /translation="QARMGSTRLPGKILKKLEDKPVLAWVITRLKQCMNLNDIIVATT
                     KDPLDDIVVRTAHILEVPFYRGATEDVLSRFYYAAIEHNADIIVRICSDCPFIDYQLL
                     DQMVAHFLDINTNKHIDYLSNTLERTYPRGLDVEIFFFKTLEKAQRHAKKLHQREHVT
                     PFIYENPSAFNIQQYKTTANLAHHRWTLDTLEDWNFFETVIAQYKSDNKVLINTETII
                     ESLEQNPEIYSINQHIKQKAIQNIVNND"
BASE COUNT          997 a          616 c          546 g         1147 t
ORIGIN      
        1 aaaataattc ttatctttgt agaaggtgtc atagaaatcg gcatattggt taaatatcat
       61 gtaacatcct catagggcag attacggatt ttttcatatt ggtttctcca atctccctta
      121 cttatcgtat attgataaaa attttcttga aatgcacaac tattaagaga atttaggaaa
      181 atggcatcat aacaattttt atctacaaaa attccattaa ttccacggct ctccacagtg
      241 atgaatgaat aaccgtgcgc gttaaaaaga tggcgccacg cgttgatact tacaccataa
      301 taataattat taacattagg aacgcaattt tttgggattg taatacttaa gttcggacca
      361 aaagctgaat tatattcgac aacgcatatt tttggcctaa aatttttgga aaacagcgac
      421 tttaaaacgt ggtaatcata actatcaatg tctaaagaaa aaatatctgg atctaaaaat
      481 ggcagcttgt ttaaaataaa ttgtacttca ttttcatgta caaattgcgg aagacactca
      541 acgcctaaat taaatttttc cataagcttc gaacaaaaat ttgataactt agtattacct
      601 tctatcatga ggccattata ttttttagct atggctaacc aggacgtatt attttccaat
      661 ccatccgaag caccaatctc taaaaaaaat ttattgggct tttttaattt agctattagc
      721 tgctcaagga tgccatcttc accattttgg ctaaatcccg aaaactccca tgaatgtgga
      781 ttagttaaat ttacttctct taaaggtgct actgctgcag ctcttgttaa tgcaatgttt
      841 actcttttta gagcttgttg catgctaaga aaacttcgca gttgttttaa catggataac
      901 ggtgttccat gatgttctta aaagatttta ttacataatt ttgttcttct tctgataatc
      961 ctaaaccact gggtaaataa aaaccttgtc ttgctaaatt ttcagacaca gggtgctttt
     1021 cttctttgaa ataacccatt ttttgcaaaa ttggctgttc atgcatgcac cagaaaaagg
     1081 gacgcgtgcc aattccaagc tcttctagct gttgtataat gtgatgtctc ataagcggtt
     1141 ttgccaaaac gataccaaat acccagtaaa tattttgtgc ggacgttgtt tctgccagcg
     1201 gtatttgaat ataagggata tctttgaatg cttccgcata gcggatacct atttcgcgct
     1261 ttttcgctat attgtcatct agtcgctcta gttgcgctaa acctatagca gcttgaacgt
     1321 tggtcatacg aaaattccag cctaattccg tatgataaaa tcttttatct ttttcaaatg
     1381 ctaggtttcg aagttttttc gcctgttctg ctaatgcttt agaatctgtt aagaccatcc
     1441 caccttcgcc tgtggtgatg tgtttattgg gataaaaact cacgatgctt atatccccaa
     1501 agctaccgca agttttgctt ttataggttt gtcctataac ttcagccgca tcctcaataa
     1561 tttttaagtg atattgattt gctaattgaa ttaaaggctc catatctacg ggcaaaccat
     1621 aggtatgaac aaccataata gctttagttt ttttacttat tttatgttca attacattac
     1681 aatccatgtt ccaagtgtgt tcatcacaat caactaaaac aggtacaagt tgattttcta
     1741 taacggcttg aagacaggaa ataatacaaa acgttggcaa aataacttcg cttcctggag
     1801 gtaattttaa ggcacgtact gccacataaa gcgctgctgt accattcata actgccacgc
     1861 catattttcg acccactttg cttgcaaact gctgctcaaa ctcggctact aatggacctt
     1921 ctgaagaaat ccagccactt tcaatgcatt gcatgagatt aattttctct tgttcgccca
     1981 aaatcggagt gttaacgggt atcattttct attccttgat aagaatggca tgttcttcgc
     2041 acacaaaacg tgttttatcg ttttcacccg tgaaaggtcc ctgcttgatc tcaaacatct
     2101 cggtaggctc taacatttca aaaccgtgac caccgtaagc taacaaaatt actgatcctt
     2161 cctccaaaat cacactcccc aaataatcgg ttttggggtt gtaaaaatcg acacgaacct
     2221 ttcctttttt tataaataaa acttcctggg ttaaggttac actgcgcatc actttgttgt
     2281 gaatatgggg ctgaattttt tttccctttg gatgtgacat aaaagcaagt tgttgcgaat
     2341 aatcattagg cgttacaaag gtaattcctt cttgtttata ttcggagcga ataataatcg
     2401 cataaacaat atcctcaaat ataatttgct caatcatagt ctattcactt gatttaaaat
     2461 gttaattgca caattttgta aaacagaaag atccaataat gtcttcccta tatattaact
     2521 attatgaagg ttttgaggaa taggcttagt attttcttca atgcattaat cattatttac
     2581 tatattctgt attgcttttt gtttgatatg ttgattgatg ctatatattt cgggattttg
     2641 ttctaaagat tcaataatag tttcagtatt tatgagaact ttattatcac tcttgtattg
     2701 tgcaatgaca gtttcaaaaa aattccaatc ttctagtgta tctaatgtcc aacgatggtg
     2761 tgcaaggttt gccgttgttt tatattgttg aatgttaaat gctgaaggat tctcataaat
     2821 aaaaggtgta acatgttctc gctgatgtaa tttttttgca tgacgctgag ctttttccag
     2881 ggttttaaaa aaaaatattt caacatccaa accacgggga tatgtccgtt ccagcgtgtt
     2941 agaaagataa tcaatatgtt tgttagtatt gatatcgaga aagtgtgcaa ccatttgatc
     3001 caataattga taatctataa aaggacaatc gctacagatt cgaacgatta tatctgcatt
     3061 atgctcaatt gctgcataat aaaatctaga taaaacatct tccgtggctc ccctatagaa
     3121 aggcacttct aatatatgag ccgtgcgtac cacaatatca tctaatggat ctttcgtggt
     3181 tgctacaatt atgtcgttta agttcatgca ttgttttagt ctggtaatta cccaggccaa
     3241 aactggctta tcttcgagtt tttttaatat ctttccgggt aaacgggtag agcccatgcg
     3301 cgcttg
//
LOCUS       DASTIB010000143         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_879786, whole genome shotgun sequence.
ACCESSION   DASTIB010000143 DASTIB010000000
VERSION     DASTIB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879786"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFP93_02440"
     CDS             <1..862
                     /locus_tag="VFP93_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007988328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET9843501.1"
                     /translation="AHELGVDLSKIQGTGRKNRLISEDVKSYVKTVLQGKKETQGVSI
                     EPFPEIDFSQWGEIEVQELNRIKKLTAKNLHRNWVNIPHVTHFDEADITELETFRQDN
                     KHAAKNMGINLTPLAFIMKAAISSLQQYTQFNSSLSADGSRIIKKRYFHIGVAVDTPN
                     GLVVPVVRDVDKKGVFSIAKELGELSAKAREGKLKAQEMQGSCFSISSLGSIGGTNFT
                     PIINAPDVAILGVSKAAHKPIYQDQQFNARLMLPLALSYDHRLIDGAEAARFLRHLCI
                     QLEDIRKLLL"
     gene            882..>2126
                     /gene="lpdA"
                     /locus_tag="VFP93_02445"
     CDS             882..>2126
                     /gene="lpdA"
                     /locus_tag="VFP93_02445"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9843502.1"
                     /translation="MRKTMQAKVVVLGSGPGGYSAAFRAADLLGEGVVLVERYPILGG
                     VCLNVGCIPSKALLHLAEIKREAQVLSSHGIEFGTPTVSIEKIRDYKNKVVKRLTSGL
                     SQMAKMRKVEVVTGFGTFNSSQELHVKSENEEISIQFEHAIIAAGSSPIKLPFLPDDP
                     RIMDSTDALDLEDIPERLLVIGGGIIGMEMATVYSALGSKVTIVELTDSILAGVDKDI
                     VTPLFKRFKPECENILLKTKVTKAQAQKDGIYVTFEGENAPPNAERFDKVLASVGRAP
                     NGKTLQVEKAGVQLDEKGFIPVDKQQRTNVAHIFAIGDIVGQPMLAHKAIPEGRVAAE
                     VIAGKKHFFDPKCIANVAYTDPEVAWVGLTENAAKQNQISYETGIFPWMASGRSLSMG
                     REEGITKIIFDKNTHKVLGGAIV"
BASE COUNT          674 a          383 c          487 g          582 t
ORIGIN      
        1 tgcacacgag ttaggggtgg atctttccaa gattcaagga acagggcgaa aaaatcgtct
       61 tatttccgaa gatgtaaaat cgtatgtaaa aaccgttttg caaggaaaaa aagagacaca
      121 gggtgtgtcc attgaacctt ttccagagat tgattttagc caatggggtg agattgaggt
      181 tcaagagctt aaccgcataa aaaaacttac tgcgaaaaat ttacatcgaa actgggtgaa
      241 tattccgcat gttacccatt tcgatgaggc ggatataacg gagcttgaga cattccgtca
      301 agataacaaa catgcggcaa aaaatatggg gattaattta acccctttag ctttcatcat
      361 gaaggccgcg atttcgagtt tacaacagta tacgcaattt aatagttcct tatcggcaga
      421 tggttctcgc atcattaaaa agcgctattt tcatataggt gttgccgtgg ataccccgaa
      481 tggattagtg gtgcctgtgg tgcgagatgt ggataaaaaa ggcgtatttt ctatagcaaa
      541 agaattgggt gaactcagtg caaaagctcg agagggcaaa ttaaaagcac aagaaatgca
      601 aggttcctgt ttttccattt cgagtttagg ttctatcggt ggcactaact ttacccctat
      661 tatcaatgca ccggatgttg cgattttagg agtttccaag gcggcacaca agccgatata
      721 ccaagatcaa caatttaatg caagattaat gcttccgttg gcattatctt acgatcatcg
      781 tcttattgac ggtgcagaag cagcacgttt cttgcggcat ctttgcatac aactggaaga
      841 tataagaaaa ttattactct aaaaataaaa aggggatgat tgtgcgtaaa accatgcaag
      901 ccaaagttgt agtacttggc agcggcccgg gcgggtatag cgctgcattt cgcgcggctg
      961 atttattagg cgaaggggtg gtactggtag aacgttatcc tatccttgga ggcgtttgtt
     1021 taaatgtggg atgcattcct tccaaagcgt tgttgcatct agctgaaatc aaacgtgaag
     1081 cgcaagtatt atcctcgcat ggcattgagt ttggaacgcc cacggtaagt atagaaaaaa
     1141 ttcgtgatta taaaaataaa gtggtaaaac ggttaacttc tggtttaagc caaatggcaa
     1201 aaatgcgtaa agtagaggtt gtaacgggtt ttggaacttt taattcttcg caggaactcc
     1261 atgtaaaaag tgaaaatgaa gaaatttcca ttcaatttga acatgcaatt attgcagcag
     1321 gatcaagccc tataaaatta ccttttcttc cggatgatcc gcgcatcatg gattccacag
     1381 atgcattaga tttggaagat attcctgaaa gattgttggt gattgggggt ggcatcatcg
     1441 gcatggaaat ggcaaccgtt tatagcgctt taggatcgaa agtaacaatt gttgaattaa
     1501 cagattccat tttagctggt gtcgataaag acattgtgac acctcttttt aaacgattta
     1561 aaccagaatg cgaaaacatt cttctcaaga ctaaagttac taaagcacaa gcgcaaaaag
     1621 atggaatata tgttacgttt gaaggagaaa atgcccctcc taacgcagag cgtttcgata
     1681 aagtgttggc atcagttggt cgagcaccga atggcaaaac actgcaagtc gaaaaagcag
     1741 gtgttcaact agatgaaaaa gggtttattc ctgtagataa gcaacaaaga accaatgtag
     1801 cgcacatctt tgccattggt gatattgtag gccagcctat gctagcacat aaagctattc
     1861 ctgaaggtcg cgtcgctgca gaagtcattg ccggtaaaaa acattttttt gatcctaaat
     1921 gtattgcgaa tgtcgcctat accgatccag aagtcgcatg ggttggtctc accgaaaatg
     1981 cagctaaaca aaaccaaatt tcctatgaaa caggcatatt tccttggatg gcttcaggca
     2041 gatcattgtc gatgggtaga gaggaaggaa taactaaaat aatcttcgat aaaaataccc
     2101 ataaggtatt agggggtgct atcgta
//
LOCUS       DASTIB010000144         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_957369, whole genome shotgun sequence.
ACCESSION   DASTIB010000144 DASTIB010000000
VERSION     DASTIB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957369"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFP93_02450"
     CDS             complement(<1..648)
                     /locus_tag="VFP93_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HET9843503.1"
                     /translation="MQFLLRKLRFIYHPPTFIQFSRKMLPGIMFCAFVLFCYSFWGAL
                     YHSPPDYQQGEGVRILYIHVPCAILSLAIYTSIAMLSFVYLVWRIKIYDLFAKELANL
                     GAIYTLLALVTGSLWGKPMWGTWWVWDARLTSELILLFLYLAYILLRQSLPNPKQGAK
                     ACAMLAMVGFIDIPIVHFSVEWFQTLHQGGTLFLLKKTKMHPDMVKPLLGMLAGFF"
     gene            complement(650..1294)
                     /locus_tag="VFP93_02455"
     CDS             complement(650..1294)
                     /locus_tag="VFP93_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET9843504.1"
                     /translation="MFKREWLITRRSKGQWAQPIAFFILATAILPMSQGQRLTQLPLL
                     SCSLLWMTTLLASLLTLDGVLRSDHQEGCFEQWILGSYPLWWLMLIKSWAHWVIWVLP
                     MVLISPLLMIMLQIPVSAIPILFISLLLGTPSFSFLGLLSTSLTLSIKASGLLLALIL
                     IPLAYPVILMGLAPTTRSLEGFGVGGNFALLAAFSIVMSVLAPHVCAFGVKVSI"
     gene            complement(1309..1917)
                     /gene="ccmA"
                     /locus_tag="VFP93_02460"
     CDS             complement(1309..1917)
                     /gene="ccmA"
                     /locus_tag="VFP93_02460"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HET9843505.1"
                     /translation="MTLTVSNISISYGQHFALDSVCFSVNSGETLKLYGANGSGKSTL
                     LRILAGLLEPDSGKYTLPPLQLPFQLPICYLGHKDGLKSVFSIQEIFTLELGLVGIDI
                     QAHDIESKLTYWGLAAKSADKIIAELSAGQRRRVTFALLQTKNAMIWLLDEPFVGLDS
                     KGKEKVQEAILAHCQKGGIVILTQHEKSDTGLTYHVNGTAKC"
     gene            2074..>3013
                     /gene="sohB"
                     /locus_tag="VFP93_02465"
     CDS             2074..>3013
                     /gene="sohB"
                     /locus_tag="VFP93_02465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HET9843506.1"
                     /translation="MLDFLFHYGLFVAKTVTICILIVLTFGFIFSIIARAKRSEGSFN
                     ILNINEKLEDIQESFERETLPKKKFKDFIKQRKKALKQQKTDENKKRLFVIKFDGDIR
                     ATQVAELREIITAVLATANSNDEVLVILESAGGFVHQYGLAASQLKRIRDRGILLTVA
                     IDKLAASGGYLMACVANKIIAAPFAIVGSIGVVGQMPNFHKLLEKNHIEFEQHTAGEH
                     KRTITMFGKNTEKDRKKFREELDLTHQLFKDFIDEHRPIVDLAKVATGEHWHASYAMQ
                     FNLVDAVMTSDDYILSNHPAKAIFEISYITKHTLKDK"
BASE COUNT         1029 a          598 c          563 g          823 t
ORIGIN      
        1 gaaaaaacct gctaacatgc cgagcaatgg ctttaccata tccggatgca ttttggtttt
       61 tttgagtaaa aataatgtac caccctggtg caatgtttga aaccattcta ccgaaaaatg
      121 tacaatgggg atatcaataa aacctaccat ggctaacatt gcgcatgctt ttgcaccttg
      181 tttgggatta ggcaaagatt gtctcaacaa tatataagcc aaatacaaaa acaaaagaat
      241 tagctcagaa gttaaacgcg catcccacac ccaccaagta ccccacatgg gtttacccca
      301 taaactgcca gtaactaaag caagcaaagt atatattgcg cctaaatttg caagctcttt
      361 agcaaagaga tcataaattt taatacgcca tacaagatac acaaacgaaa gcatagcgat
      421 acttgtgtat attgctaaag ataaaattgc gcagggtacg tgtatatata aaatacgcac
      481 gccctcacct tgttgataat ccggtgggga atggtataac gctccccaaa aactgtaaca
      541 aaatagtaca aaagcacaga acattatacc tggcagcatt ttccgagaaa attgtataaa
      601 agtgggcgga tgatatataa atcgcagttt acgaagcaaa aactgcatat taaatactca
      661 ccttaacacc gaaggcacac acgtgcggtg ccaaaacact catgacaata ctaaaagctg
      721 ctaatagggc aaagttaccg ccaacgccaa atccttctaa ggatcgggta gtgggtgcta
      781 accccattaa aattacagga taggcaagcg gaattaaaat gagtgcgaga agtaaaccac
      841 ttgctttgat ggaaagcgtc aaagaagtgc ttaacaaacc taaaaagcta aagcttggtg
      901 taccaagcag taagcttata aatagaatgg gtatggcaga cacaggaatt tgtaacataa
      961 tcataagcaa agggctaatc aataccatgg gtaataccca tataacccaa tgtgcccatg
     1021 attttatcaa catcaaccac caaagaggat aagaacctaa tatccattgc tcgaaacatc
     1081 cttcttgatg gtcggatcga agcacaccat cgagcgttaa caacgatgct aataatgtgg
     1141 tcatccaaag aagactgcaa ctcaataagg gtagttgcgt taagcgttga ccttgactca
     1201 tcggtaaaat agctgttgct aaaataaaaa aagcgatcgg ctgagcccac tgacctttag
     1261 atcggcgtgt tataagccac tcgcgtttga acattaccca agctacattt agcactttgc
     1321 agttccatta acatgatatg ttaatcctgt atccgatttt tcatgttgcg tcagaatgac
     1381 aatcccccct ttttgacaat gcgcaagaat tgcctcttgt actttttctt ttccttttga
     1441 atctaatccc acaaaaggtt catccaataa ccaaatcatg gcattttttg tttgtaataa
     1501 agcaaatgtg actctgcgac gctgaccagc ggaaagttca gcaataattt tatcggcgct
     1561 cttcgcagca agcccccaat aggtaagttt actttctatg tcatgtgctt gtatatcgat
     1621 acccactaaa ccaagttcta aggtaaaaat ttcttgaatg ctaaacacac tttttaaacc
     1681 atctttatgt cccaaataac aaatgggcaa ctgaaacggc aattgtaatg gtggcaatgt
     1741 gtatttgcca ctgtccggtt ctagcaaccc cgctagtatg cgtaacaaag ttgatttacc
     1801 agaaccatta gccccataaa gctttaatgt ttcaccagaa tttacgctaa aacaaacgct
     1861 atccagtgca aaatgttgcc cgtaacttat agaaatatta gaaaccgtaa gtgtcacacg
     1921 gaaagacctt ttacctttgc agattaggct atcataaagt cacgcaaaat tacagtcaga
     1981 aaactaagga tggcaaatat ttgcaggagt tgttcggtcg ctttaaaatg aagtttgaca
     2041 tacaatccga gttatttatc taaaagagta tctatgttag atttcctttt ccattatggt
     2101 ttatttgtcg caaaaacggt tacgatttgc attctcattg ttcttacctt tggctttatt
     2161 ttttccatta tagcgcgtgc aaagcgcagc gaaggtagtt ttaatatttt aaatattaac
     2221 gaaaaacttg aggatatcca agaaagcttc gaacgagaaa ctttgcccaa gaaaaaattt
     2281 aaagatttca tcaagcaacg caaaaaagcg ttaaaacaac aaaaaacaga cgaaaataaa
     2341 aaacgtttgt ttgttataaa gtttgatggg gatattcgag ctacccaagt agcggaactg
     2401 cgtgaaatta ttactgccgt attagctacc gcgaattcaa acgatgaagt tcttgttatt
     2461 ttggaaagtg cgggtggctt tgtgcaccaa tatggattag ccgcatcgca attaaaacgt
     2521 attcgcgatc gtggcatact tttaaccgtt gcaattgata aattggcagc gagtggtggg
     2581 tatttaatgg catgtgttgc caataaaatt attgccgccc cttttgcgat cgtgggatcc
     2641 attggggttg tcggccaaat gccgaatttt cacaagctgc tggaaaaaaa tcatatagaa
     2701 tttgagcaac atacggcagg cgaacataaa cgcactatca ctatgtttgg caaaaacaca
     2761 gaaaaagatc ggaaaaaatt tcgcgaggaa ctagatctta cgcatcaatt atttaaagat
     2821 tttatcgatg aacatcgacc gattgtggat ttagctaaag tggccacagg agaacattgg
     2881 catgccagtt atgcgatgca atttaattta gtagatgcag tcatgacaag tgatgactat
     2941 atattaagta atcatcctgc caaagcaatt tttgaaatct cttatatcac caaacataca
     3001 ttaaaagata aat
//
LOCUS       DASTIB010000145         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1293103, whole genome shotgun sequence.
ACCESSION   DASTIB010000145 DASTIB010000000
VERSION     DASTIB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293103"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFP93_02470"
     CDS             <1..298
                     /locus_tag="VFP93_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9843507.1"
                     /translation="TKSELEKYVDYLASHPNTHVRIEGHADERGTTAYNLGLSKKRAS
                     AVANYLLDRGVAKNQIEVMSFGAQKPLASGNSEEAYAKNRRAEVQFEMNQDSAA"
     gene            complement(393..2096)
                     /locus_tag="VFP93_02475"
     CDS             complement(393..2096)
                     /locus_tag="VFP93_02475"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9843508.1"
                     /translation="MRTSSYLLATVKETPKDAELISHQLMIRAGFIRKLAAGLYSWLP
                     LGLRVLRKVEAVVRQEMDAIGAQEVLMPAIQPAELWIETQRWDAFGPQLLKLKDRHDR
                     DFCYGPTHEEVITDLIRKELRSYKQVPCCFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHLNKESLEQTYQAMYAAYSKIFTRLGLKFRAVLADTGAIGGAHSHEFHVLADSGE
                     DAIAYSTEGDYAANVELATSAPIQSTHSISHQTLQLIETPNITTVEDQANFLNIKTEQ
                     ILKTLLVKGIEHEAVALLLRGDDELNPVKAEKSPLIAKPLTKITVEELQAIAGCNPGF
                     VGPKDLKIPVIADARVLHMHNFSCGANQNDKHYIGVNWERDLPIPDTIMDLRQVKEND
                     ASPDGKGVLKICRGIEVGHIFQLGNKYSKSMHASVLDEQGKNQLLEMGCYGIGVSRIV
                     AAAIEQSHDAKGILWSEEMAPFSIAIVPMHLHKSHRVQEAAEKLYQEMVDAGFEVLFD
                     DRKERPGVIFNDLDLIGIPHRFVISERGIDQDLLEYKNRRAEEPMLIPFSQWREALKT
                     I"
BASE COUNT          651 a          479 c          444 g          689 t
ORIGIN      
        1 aactaaatcc gaattagaaa agtatgtaga ttatttagca tcgcacccca acacccatgt
       61 tcgtattgaa ggacatgctg atgagcgggg tacaactgca tacaatcttg gtcttagcaa
      121 aaaacgtgca tccgcagttg caaactattt attagatcgc ggtgtcgcta aaaatcaaat
      181 agaagtcatg agtttcggtg cccaaaaacc tcttgcttcc ggtaactctg aagaagccta
      241 tgctaagaat cgccgagctg aagtacaatt tgaaatgaat caagattctg cagcgtaaat
      301 gcatcttaaa taaatttgtt ttacctctac aagtatttca tacgcgtaga ggttttcatg
      361 aagcaaaaat gaagcacgat tgtgcttttt atttaaatag ttttaagtgc ttctctccat
      421 tgggaaaacg gaattaacat aggctcttct gcacgacgat ttttatattc aagtagatct
      481 tgatcgatac cgcgttcact tattacaaaa cgatggggga tcccgatgag atccaaatca
      541 ttaaaaataa cgccgggtct ttctttgcga tcatcaaaca atacttcaaa acccgcatcg
      601 accatttctt gataaagttt ttccgcggcc tcctgcacac ggtgtgattt atgcaaatgc
      661 ataggcacga tagcaataga aaaaggtgcc atttcttcgg accacaaaat cccttttgca
      721 tcgtggcttt gttctatagc cgcagcaaca atgcgtgaaa cgccaatacc ataacaaccc
      781 atttctaaaa gttggttctt tccttgttca tctaaaacac tcgcatgcat acttttgcta
      841 tatttgttgc cgagttgaaa aatgtgacca acctcaatac cacgacaaat ttttaacaca
      901 ccctttccat caggacttgc atcgttctct tttacttgac gcaaatccat aattgtatct
      961 ggaattggca aatcgcgctc ccaattcaca ccgatataat gtttatcgtt ttgattagca
     1021 ccacacgaga aattatgcat gtgtaatacg cgcgcatctg caattactgg gatcttaaga
     1081 tctttaggac cgacaaaacc gggattacac cctgcaatgg cttggagttc ttctacggtg
     1141 attttagtta aaggttttgc aataagggga gatttttctg cctttacggg gtttaactca
     1201 tcatctccgc gtagtaaaag tgctactgcc tcgtgctcaa tgccttttac taagagcgtt
     1261 tttaagattt gttctgtttt aatgtttaag aaattggctt gatcttcaac cgtggtaatg
     1321 ttgggtgttt ctatgagctg caaagtttga tgggagatag aatgtgtact ctgtatagga
     1381 gcacttgtag caagttccac atttgcagca taatcccctt cggtcgaata ggcaatggca
     1441 tcttcgcctg aatccgctaa tacatgaaac tcatgcgaat gtgcaccacc gatagcaccc
     1501 gtgtctgcta atactgcacg aaatttaaga ccaagtcggg taaaaatttt gctataagct
     1561 gcgtacattg cctgataagt ttgttctagt gattctttat ttaagtggaa agaatacgca
     1621 tctttcatga taaattcacg tgcacgcatt acgccaaagc gcgggcgaat ttcatcgcga
     1681 aatttggttt gaatctgata aaaacaacaa ggcacttgct tatagctgcg cagttcttta
     1741 cgaataagat cggtaattac ttcttcatgg gttggtccat agcaaaaatc gcgatcgtgg
     1801 cgatctttca gtttcaataa ttgtggccca aaggcatccc atcgttgcgt ttcgatccaa
     1861 agttctgccg gttgaatggc gggcattaat acttcttgtg caccaatcgc gtccatttct
     1921 tgacgaacta cggcttctac ttttcttaat acgcgtaaac ctagggggag ccaagagtac
     1981 agtcccgccg ccaattttcg aataaatcct gctcgtatca ttaattgatg gctaattaat
     2041 tccgcatctt ttggcgtttc tttaacggtc gctaataaat aggaagaagt gcgcataagt
     2101 ttctccctaa gccaattctt tcagataagt agaatgcgga aaatttaagc gcatgatgcg
     2161 caacgaatcc tcagcaagat catgcatttt taactgtcga tacgataata tttggatgta
     2221 gagagcttct tctacactag gtgcttgatc aaaatgttgc act
//
LOCUS       DASTIB010000146         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_5803, whole genome shotgun sequence.
ACCESSION   DASTIB010000146 DASTIB010000000
VERSION     DASTIB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5803"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /gene="rplN"
                     /locus_tag="VFP93_02480"
     CDS             complement(<1..309)
                     /gene="rplN"
                     /locus_tag="VFP93_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9843509.1"
                     /translation="MIQMQSVLDVADNSGARRVMCIKVLGGSKRRYAGIGDVIRVTVK
                     EAIPRGKVKKGDVLFAVVVRTRKGVRREDGSLIRFDGNAAVLLNPQQQPIGTRIFGPV
                     T"
     gene            complement(368..631)
                     /gene="rpsQ"
                     /locus_tag="VFP93_02485"
     CDS             complement(368..631)
                     /gene="rpsQ"
                     /locus_tag="VFP93_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9843510.1"
                     /translation="MAENTVRRALIGKVLSDKRNKTRTVEIRSSSRHPQYGKVVSKIT
                     KCQVHDEMNESKEGDLVEICETRPISKTKTWKLVRVVEQARII"
     gene            complement(634..825)
                     /gene="rpmC"
                     /locus_tag="VFP93_02490"
     CDS             complement(634..825)
                     /gene="rpmC"
                     /locus_tag="VFP93_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9843511.1"
                     /translation="MIAKQLRQKELPELNEELKKLIQEGFKLKMRHGSGQLNQNHLLK
                     NNRKAIAQIKTVIHERERG"
     gene            complement(825..1238)
                     /gene="rplP"
                     /locus_tag="VFP93_02495"
     CDS             complement(825..1238)
                     /gene="rplP"
                     /locus_tag="VFP93_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9843512.1"
                     /translation="MLQPKRTKFRKQMKGRNRGLAQRGNKVSFGEFGLRATGRGRLTS
                     REIEAARRAMTRHIKRGGKIWIRVFPDKPITKKPLEVRMGKGKGNVEEWVAQIKPGKM
                     LYEIEGVSREIAERAFQLAAAKLSINAEFVERTVM"
     gene            complement(1245..1937)
                     /gene="rpsC"
                     /locus_tag="VFP93_02500"
     CDS             complement(1245..1937)
                     /gene="rpsC"
                     /locus_tag="VFP93_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET9843513.1"
                     /translation="MGHKVHPIGIRLGIIERHRSMWFANSKDYSKKLQGDIRTRDFLK
                     KKLANAQVARIVIERLSNNGKITVYTARPGVVIGKKGEDIESLRQQASQLMGVPVNIT
                     IEEIRRPELEATLVAESIAQQLERRVMFRRAMKRAVTTALRLGAEGIKICVSGRLGGA
                     EIARTEWYREGRVPLHTFRAHIDYAQARANTTYGVIGVKVWIFKGEIFDKKALEQNRG
                     EGVSLQAEGQKG"
     gene            complement(1948..>2078)
                     /locus_tag="VFP93_02505"
     CDS             complement(1948..>2078)
                     /locus_tag="VFP93_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HET9843514.1"
                     /translation="ETICVDEAPVLKRFHARAKGRGNRILKRKCHIKIVVSEIEQK"
BASE COUNT          523 a          526 c          342 g          687 t
ORIGIN      
        1 cgttacggga ccaaaaattc gtgtgccaat gggctgttgc tggggattta ataatacagc
       61 tgcatttcca tcaaagcgta taagtgagcc atcttcacga cgcactcctt tccttgttcg
      121 aactacaact gcgaataata catcaccctt tttaactttt cctcgaggga tagcttcttt
      181 tacagttacg cgaatcacat caccaatacc tgcgtatcga cgcttcgatc cacctaaaac
      241 tttgatgcac atcactcgac gagcgccact attatcggca acgtcaagaa cgctttgcat
      301 ctgaatcatt ctcgttctcc gttatctcac tgctatttag ccaaaatcaa atcacgcaat
      361 ttaatgacta aattatacgc gcttgttcaa ccactctcac aagtttccag gttttggttt
      421 ttgaaattgg gcgggtttca caaatttcaa ccaaatcacc ttctttactt tcattcattt
      481 catcatgcac ttggcatttg gttattttac ttacaacctt gccgtattgt ggatgacgtg
      541 aagatgaacg aatttcaact gttctagttt tattacgttt atcactcaat actttaccaa
      601 ttaacgcacg tcgtacggta ttctcagcca ttatcagccc cgctctcttt catgaattac
      661 ggttttaatt tgagcaattg ctttacgatt gttttttaac agatgatttt gattcaactg
      721 cccagaaccg tgacgcattt ttaatttaaa accttcttgt attaattttt tgagttcctc
      781 atttaattca ggcaactctt tttgacgtaa ttgttttgca atcattacat caccgtcctt
      841 tctacaaatt ctgcgttaat cgataacttc gctgccgcaa gttgaaacgc gcgctcagca
      901 atctctctag aaacgccttc gatttcataa agcattttgc ccggtttaat ttgagcaacc
      961 cattcttcca cgttaccttt acctttaccc attctaactt ccaatggttt ttttgtaatt
     1021 ggcttatctg gaaaaactct tatccaaatt tttccaccac gtttaatgtg gcgagtcatg
     1081 gcccgacgtg ctgcttcaat ttcacgggaa gtaagtctac ccctgcccgt agcccgaagt
     1141 ccaaattctc caaaactaac tttgttgcct ctttgagcca agccacggtt gcggcccttc
     1201 atttgctttc taaatttagt tcgcttaggc tgtaacattt tttactaccc cttttgccct
     1261 tcggcctgca agctcactcc ttcgcctcta ttttgctcaa gagctttttt atcaaaaatt
     1321 tcgcccttaa atatccatac tttgacacca atcacaccat acgtagtatt agctcgcgct
     1381 tgcgcataat cgatgtgtgc tcgaaaagta tgtaatggaa ctctgccttc gcggtaccat
     1441 tctgtgcgcg caatttccgc cccacccagg cgacccgaaa cgcaaatttt aattccctct
     1501 gcacctaatc gaagtgcagt agttactgcg cgtttcatag cccttctgaa cataactcga
     1561 cgctctaact gttgcgctat gctttcagca actaacgttg cttctagttc tggacgacga
     1621 atttcttcga tagtaatgtt aacaggcact cccattaatt gacttgcctg ttggcgcaag
     1681 gattcgatgt cctcaccttt tttaccaatt acaacaccag gacgcgcggt gtaaacagta
     1741 atttttccat tatttgacaa tcgttcaatt actatgcggg ctacttgggc atttgccaac
     1801 ttcttcttca agaaatcgcg ggtacgaata tcaccttgta acttttttga ataatctttc
     1861 gaattggcaa accacatgga ccgatgtcgc tctatgatgc ctaagcgaat tcctattgga
     1921 tgaactttat gacccatcta cgcctcgcta tttttgctca atctcagata ccacaatttt
     1981 aatgtggcac ttgcgtttta aaattctgtt tccacggccc tttgcgcgag catgaaaacg
     2041 cttcaacaca ggagcttcat cgacacaaat agtttcaa
//
LOCUS       DASTIB010000147         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1372875, whole genome shotgun sequence.
ACCESSION   DASTIB010000147 DASTIB010000000
VERSION     DASTIB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372875"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFP93_02510"
     CDS             <1..741
                     /locus_tag="VFP93_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843515.1"
                     /translation="RKRELNLNIPAIAQLLKKVEQGCILCYKNILQLQSLLQNRTYKD
                     LNHHEVINQVHSLETETKSLTQCMVALNEMALKRDPLYQLFEAELFKTLMHLESSDKL
                     PASHLAQYYERGSGLRDKAHRIKDAEKRKIAFNLISYYETLKRASNLSQNDASLQSIE
                     MFKQHAELFFGASANADTENQGKELIVLLFNDLEAMRFLNRTGIFEKSSAIRKQFKQL
                     LQQSLFGKECLKELQMSPRSKMLLRTKF"
     gene            complement(742..1110)
                     /locus_tag="VFP93_02515"
     CDS             complement(742..1110)
                     /locus_tag="VFP93_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HET9843516.1"
                     /translation="MIVAEESHVAKFRLQAFAPGKVKLNDTWHEKSIFLHEDTCKFLC
                     CNTLQEFIALDLHEYLAHSPSIVLLGTGPTLKIPPEVLRAFFLQRGIGLEFMNSYAAC
                     RTYAVLQSEMRAVGVFVLTG"
     gene            1256..2278
                     /locus_tag="VFP93_02520"
     CDS             1256..2278
                     /locus_tag="VFP93_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843517.1"
                     /translation="MIDVLHIILPNLFSSLPANFLKLNSYPALKKLLKKAKRIPDQPP
                     YLEGVLKNHFSGLKCAELPSGALGAFSLGLHHKTSKQVWMRCDPVHFKTDFSGVYCLG
                     NKNLSLSQQEICALEKALNEHFMHSGMQLFTFPNNEWYLSLPDNSDIATNDLEEIVGH
                     SISHVLPTGSDQIFWHRLFTECQLLLKTHPVNIAREKLNLPQVQGVWFWGKGSIPREL
                     MTHFDFVSTNMRAIQGMTKLCEKKSIPFSRQFEKMLTANAGRCGAIIDGDLRGSADKT
                     QDSVVKKAILEEYDEMLFKPLCDALLRKRIKQVYLDGCDGNVYYLTRSSFKYFWWRKY
                     LSSTSA"
     gene            2275..>2585
                     /locus_tag="VFP93_02525"
     CDS             2275..>2585
                     /locus_tag="VFP93_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843518.1"
                     /translation="MNTRAKIPKPAILHRPIQLEVYKKAQGLNLDVLVSRILAGRSLP
                     LEVDLNAWLYGTLQNIHSPKLMADLQKSTQRLAHAIKSGECIGIETDHDCDGQTSHAV
                     I"
BASE COUNT          846 a          461 c          485 g          793 t
ORIGIN      
        1 agaaaaaggg aattaaatct taatataccc gctatcgcgc aacttttaaa aaaggtggaa
       61 caaggttgca ttttatgtta caaaaacatt ttacagttac agtctttatt acaaaatcgt
      121 acctataaag atttaaacca tcacgaagtt attaatcagg tacattccct agaaacagaa
      181 acaaaatcgt taacgcaatg tatggtagcg ctgaacgaaa tggctttaaa acgtgaccct
      241 ctgtatcaac tattcgaagc tgaattattt aaaactttaa tgcatttaga atcttccgat
      301 aagctacctg cttcgcattt agcgcaatac tatgagcgag gttcaggttt aagagacaaa
      361 gcacatagga taaaagatgc agaaaagaga aaaattgctt ttaatttaat ttcctattat
      421 gaaacgttga agcgtgcgag taatttatct cagaatgacg cgtcccttca atcgatagaa
      481 atgtttaaac aacatgcaga gctttttttt ggagcttcgg caaatgcgga taccgaaaat
      541 caaggcaaag aattaatcgt gttattattt aatgatctgg aagcgatgcg ttttctcaat
      601 cgcacgggaa tttttgagaa atcgagtgcc atacgcaaac aatttaaaca attattgcaa
      661 caatcgttgt ttggcaagga atgtctaaaa gaactgcaaa tgagcccccg ttctaaaatg
      721 ttgttaagaa ctaaattttg attatccagt aagtacaaaa acgccaaccg cacgcatttc
      781 cgattgtaat actgcatacg ttcgacatgc ggcgtagcta ttcataaatt ctaaaccaat
      841 gcctcgttgt aaaaaaaatg cgcgtaagac ttccggtggt attttgagtg taggaccagt
      901 acctagtagc acaatggatg gactatgcgc caaatattcg tgtaaatcga gtgcaataaa
      961 ttcttgtaat gtattacaac ataaaaattt acacgtatct tcgtgcaaaa aaatactttt
     1021 ttcgtgccat gtatcattca gtttgacttt tcccggggca aaagcctgta ggcgaaattt
     1081 tgctacgtgt gattcttctg ctactatcat gatcctacta ctttaactca actatcaaat
     1141 tattttgtta ggaaactttg tttcttgtct aattttaaga ttttaaacct accccaggta
     1201 tttggcaaag tagagaaaac tcgtgtagtc tgttgtgaaa caaaaaagaa aaaatatgat
     1261 cgacgtttta catataattt tgcctaattt attttcaagt ttgcctgcca actttctcaa
     1321 attaaatagt tatcctgctc tgaaaaagtt gctgaaaaaa gcaaaacgca tacccgacca
     1381 acctccatat ttagaaggtg tgttaaaaaa tcatttttct ggcttaaaat gcgcggaatt
     1441 accctcgggt gctttgggtg cttttagtct tggtttgcac cataaaacca gcaaacaagt
     1501 ttggatgcgc tgtgatccgg tgcattttaa aaccgatttc tctggagtct attgtttagg
     1561 gaataaaaat ttaagccttt cacaacaaga aatctgtgcg ttagaaaaag ccttgaatga
     1621 acattttatg cattctggta tgcaattatt tacttttcca aataacgaat ggtatttgtc
     1681 actacctgac aactcggata tagctacgaa tgatcttgag gaaattgtag gtcattctat
     1741 ttcccatgtt cttcctacag gaagcgacca aatattttgg catcgtttat ttacggaatg
     1801 ccaactatta ttaaaaacac atcctgttaa tattgctcgt gaaaagctga atctacccca
     1861 agtacaaggt gtttggtttt ggggcaaagg atccatacca cgagaactta tgacgcattt
     1921 tgattttgta agcaccaata tgcgtgcaat acaaggtatg acaaaattgt gtgaaaaaaa
     1981 gagcattccg ttctctcggc agtttgaaaa aatgttaacc gcaaacgctg ggcggtgtgg
     2041 cgcaattatt gatggagatt taagaggcag cgctgacaaa acgcaagatt ctgtcgtaaa
     2101 aaaagctatt cttgaggaat atgatgaaat gctttttaaa cctttatgtg atgcattgtt
     2161 gcgcaaaaga ataaagcaag tatacctgga tggatgtgat ggtaatgtgt attacttaac
     2221 gagatcctct ttcaaatatt tttggtggcg taaatactta tcgtcaactt ccgcatgaat
     2281 acaagagcaa aaatccctaa acctgccatt ttgcatcgtc ctattcaatt agaggtttat
     2341 aaaaaagccc aagggcttaa tttagatgtt ctagtctcaa gaattttagc aggtcgatct
     2401 ttacctttgg aagtggattt aaatgcttgg ctctatggta ctttacaaaa catccacagt
     2461 cctaaactta tggcggattt gcaaaaatca acacaacgtt tggcgcacgc tataaaatcg
     2521 ggcgaatgta ttggtattga aaccgatcat gattgcgatg gtcaaaccag tcatgcggtg
     2581 attta
//
LOCUS       DASTIB010000148         5887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_922028, whole genome shotgun sequence.
ACCESSION   DASTIB010000148 DASTIB010000000
VERSION     DASTIB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922028"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFP93_02530"
     CDS             <1..1145
                     /locus_tag="VFP93_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9843519.1"
                     /translation="MLTKLDTLVFDKTGTLTEPVVQTQFFSSPDDADTIIQWAASLEN
                     SFASHPIALAIIAANRKKLCAVQDAKMHPSSQGVIGMINDKKVIVGSLALLHQENCVI
                     SLDIRRESEKLQATGFTPVFIAIEGKCQGVLGLKHQLKPHVASTLQHLKKQGFKLMLL
                     TGDHTSVTNVLAKDLPFDQVYSEKSAQQKRDIIQGLVNTKHKVGFIGDGLNDMEAIDA
                     AHMGIAIQSWTHLASKAHLCLKGSIRELPKSIELASLVQTNIKQNLAWTFFYNTLSLL
                     VASGALYPLIGFSLNPIIFAAMMGISSLVVIANSLRVPTLLDQQCEKNPKCSQNILSL
                     SSTIYPATLTKEAKVSKKPKITHKASNLLPLKSECNNQNKKFTYNF"
     gene            complement(1133..2926)
                     /locus_tag="VFP93_02535"
     CDS             complement(1133..2926)
                     /locus_tag="VFP93_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843520.1"
                     /translation="MLNNFPNLNDLAFFRNLSSNAEQLKRNQQWNNTVDKFLSPSEQF
                     FIGAKLATEQYKAILKKNRQDNPILVLRNPEQIANNIVRHAFKQLVKTEFIPGLYYKI
                     QQMLMQTDRELIPYLPANYEPNAEQYTLSSAEHAKLEQESKAIMEYIKENIPIEIYSG
                     LQECYGLHKLHKPELAEKDFINLIMFQFITSALVAYADKTLNRNNSSFALNDKRLIPL
                     ALKNIYTTGGTHVYNRQGQLQDILNAGFGNGQLKSTGFIIDNLDKKIQPLILDFDLVL
                     SKLNQIFTQIENDQIQELMQSFGVLKLDVSLKQAFVSKDSKRLSQQFKKTMECYTANI
                     LDHETFKNIIETLIEKGCPSAIYFKGHALLGCTPTGFTSPYPVNYAQGLQELLRLKEG
                     DEIFHHINVSEHSTYWKKEDIDHYTSLKDNLRKNEQMQQFLTKAYHNIYDLYANTTFT
                     HKNKVKKNIILWGMQARNYVHSNISSIRSMQITLLQNIIDNTTIAPNKKIPLLLATIE
                     AIRHQIGKTFVKSRLREIMDKLLQDLTKKSEKLYGIQVNEFTSQQKQEALYQYLKQVT
                     LDEKFKQSLITHFASSNFTQPSLIKRLSQKL"
     gene            complement(3142..4017)
                     /gene="folD"
                     /locus_tag="VFP93_02540"
     CDS             complement(3142..4017)
                     /gene="folD"
                     /locus_tag="VFP93_02540"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET9843521.1"
                     /translation="MSAQIIDGKKIAETIKQELKIQIERFVRAGYTPPGLAVIQVGED
                     AASTIYVNHKKRACLDVGIKSFSYNLHEKTSAEELINLLDSLNHQIDIHGILVQLPLP
                     EHLREKQILDFINPNKDVDGFHPYNTGRLTQGRPLLRPCTPYGIMKLFDHIHYDLKGK
                     HAVMVGASNVVGKPMALELLMAGATITICHRHTKDLISHVQQADCLIVACGKPNLVHG
                     TWIKKGAVVIDVGINRLSNGTIIGDIDFESAKVQANWITPVPGGVGPMTVAMLMTNTF
                     EAARISSNITVDLSN"
     gene            4250..4326
                     /locus_tag="VFP93_02545"
     tRNA            4250..4326
                     /locus_tag="VFP93_02545"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4284..4286,aa:Pro,seq:tgg)
     gene            4335..4411
                     /locus_tag="VFP93_02550"
     tRNA            4335..4411
                     /locus_tag="VFP93_02550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4369..4371,aa:Arg,seq:tct)
     gene            4435..4510
                     /locus_tag="VFP93_02555"
     tRNA            4435..4510
                     /locus_tag="VFP93_02555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4468..4470,aa:His,seq:gtg)
     gene            4517..4592
                     /locus_tag="VFP93_02560"
     tRNA            4517..4592
                     /locus_tag="VFP93_02560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4550..4552,aa:Lys,seq:ctt)
     gene            4786..4870
                     /locus_tag="VFP93_02565"
     tRNA            4786..4870
                     /locus_tag="VFP93_02565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4820..4822,aa:Leu,seq:tag)
     gene            4927..>5887
                     /gene="tig"
                     /locus_tag="VFP93_02570"
     CDS             4927..>5887
                     /gene="tig"
                     /locus_tag="VFP93_02570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9843522.1"
                     /translation="MEVSVENTGSLERRLTVSVPEAQINEQCRTKMAELAKDVKLKGF
                     RPGKIPPHILQQRFGKAVRAEVVGDIIQKSLENALKEKNLLPAGMPTIEQIQDESGKS
                     LEYTAKFEIFPEIKLGDLSEVEVEKKKVDITDEDIQKTITKMCENLGTWESVTRASKM
                     GDKLIIDLERRWTTQEDELQSYVKIDFSLDEKKSLPDLVNQLLDKNIDDEIDCTITYP
                     DTWHDEKIRGREATFKVKIHEILEKKPLTVEALGEKLNLDKEDPNILKEKIKERMEKE
                     ASKVIQDELKEKVLEILLEKNPIEKPKTLIEQEKKALKEEIALS"
BASE COUNT         1860 a         1017 c         1138 g         1872 t
ORIGIN      
        1 aaatgttaac taaactagat acgttggttt ttgataaaac gggcacacta acagagccgg
       61 tagtacaaac acaatttttt tcgagcccgg atgacgcgga tacgataatt caatgggctg
      121 caagtttaga aaatagtttt gcaagccatc ccattgctct tgcaatcata gcagcgaatc
      181 gaaaaaagct atgcgctgta caagatgcta aaatgcatcc ttcttctcaa ggtgtcattg
      241 gcatgataaa cgataaaaaa gtgatcgtgg gaagtcttgc attattacat caagaaaatt
      301 gtgtaataag tctcgacatt cgtcgagaaa gtgaaaaatt acaagctacc ggctttaccc
      361 cagtttttat cgctattgaa ggcaaatgtc agggagtgct aggtcttaag catcagttaa
      421 aaccacacgt ggccagtact ttacaacatt taaaaaaaca aggttttaag cttatgttat
      481 taacgggcga tcacacgtca gtgacaaatg tacttgcaaa agatttgcct tttgatcaag
      541 tgtattctga aaaatctgca caacaaaaaa gagatatcat tcaagggtta gtgaatacta
      601 aacacaaagt gggttttatt ggggatggtc taaacgacat ggaagccata gatgcagcac
      661 atatgggcat tgcaattcaa tcttggaccc atttagcttc aaaagcccac ctttgtctaa
      721 aaggaagcat ccgtgagcta cccaaaagta tcgaacttgc gagcttggtt caaaccaata
      781 taaagcaaaa tttggcttgg actttttttt ataatacgtt aagtttattg gtagcaagtg
      841 gagcgcttta tcctttaatt ggttttagtt taaatccaat aatttttgct gcaatgatgg
      901 gtatttcatc tttggtagta atagctaact cgttacgtgt gcccacactg ctggatcaac
      961 agtgtgaaaa aaacccaaaa tgttcacaga atattttaag tttatcgagc acaatttatc
     1021 ctgctaccct tacaaaagaa gccaaggttt caaaaaaacc aaaaataact cacaaagcct
     1081 cgaatctttt acccttaaag agcgagtgca ataaccaaaa taaaaaattt acttataatt
     1141 tttgagataa tcttttaatg agagatggtt gcgtgaagtt ggatgaagcg aaatgggtga
     1201 tcaagctttg tttaaacttt tcatctaggg ttacctgttt taaatattga tataaggctt
     1261 cctgcttttg ttgggatgta aattcattaa cttgtattcc ataaagtttt tcagattttt
     1321 tcgttaaatc ttgtaacaat ttgtccataa tttcgcgtaa acgactttta acaaaagttt
     1381 taccgatttg atgtcttata gcttctatag tagcaagtaa cagaggaatt tttttatttg
     1441 gcgcgatggt agtattatca attatatttt gtaaaagcgt tatttgcatc gaacgtatag
     1501 aacttatatt ggaatgtaca taattgcgtg cctgcattcc ccataggata atattttttt
     1561 ttactttatt cttatgcgtg aacgtcgtgt ttgcatataa atcataaata ttatgataag
     1621 cttttgttaa aaattgttgc atttgctcat ttttgcgcag attatctttt aaggaggtgt
     1681 aatggtctat atcctctttc ttccaataag tggaatgctc tgaaacattt atatgatgaa
     1741 aaatttcgtc gccttctttt aagcggagta attcttgcaa accttgtgca taattcacag
     1801 gataaggaga agtaaaacct gtgggtgtgc accctagtag ggcatgtccc ttaaaataga
     1861 ttgcactagg acaccctttt tcgattaatg tttcaataat atttttaaaa gtttcgtgat
     1921 caagaatatt tgccgtataa cattccatgg tttttttaaa ttgctggctt aatcgctttg
     1981 aatctttgga tacaaaagct tgtttcaaag aaacatccag tttgagcaca ccaaaagatt
     2041 gcatcagctc ttgaatttga tcattttcta tttgcgtaaa aatttgattt aattttgaca
     2101 acacaagatc aaaatctaaa attaatggct gaattttttt atccaagttg tctataataa
     2161 atcctgtgga tttaagttgg ccattgccga aaccggcatt taaaatatct tgcagttgcc
     2221 cttgacgatt ataaacatga gtacctcctg ttgtataaat attttttaac gctaatggta
     2281 ttaaacgctt atcgtttaat gcaaaggaag agttattcct attgagcgtt ttgtctgcat
     2341 aggcaactaa tgcactggta ataaactgaa acatgataag attaataaaa tctttttctg
     2401 caagttcagg tttatgcaat ttatgcagtc catagcattc ttgcaatccg ctataaattt
     2461 cgatgggaat attttctttg atatattcca taatagcttt actttcttgt tcgagctttg
     2521 catgctctgc gcttgataag gtatattgct ctgcgtttgg ttcataattc gcaggtaaat
     2581 agggtattaa ttcacgatcg gtttgcatga gcatttgctg aattttgtaa tacaaccctg
     2641 gaataaattc ggttttaact aattgtttaa atgcatgacg tactatattg tttgcgattt
     2701 gttcgggatt tcttaaaact aaaataggat tatcttgtct attttttttt aaaattgcct
     2761 tgtattgttc tgtagctaac tttgcgccga tgaaaaattg ttccgatggt gataaaaatt
     2821 tatcaacagt attgttccat tgttgattcc ttttaagttg ttccgcattg gaagaaagat
     2881 ttcggaaaaa tgctaaatca tttaagttag gaaaattatt taacataagc tgcttcgaac
     2941 caattagtga taatgtaaaa aagtattaaa taatgcaatt tttgttgcaa tataagaaat
     3001 atttatatcg tttttatttt agtataattc agtgaatatc gtatttcata cttttcaagt
     3061 ttgacgaaaa ttcaaactta taaatagaac ttttgatgtg ccagtgcaag ctgacaccaa
     3121 ttgtagtgca tcaaggtgaa cttaattact taaatccaca gtaatattgg aagaaatacg
     3181 cgctgcttca aaagtattag tcattaacat ggcaacagtc ataggtccca caccaccggg
     3241 aaccggtgta atccaatttg cctgaacttt ggcactttca aaatctatat ctccaattat
     3301 ggttccattg cttagtcgat tgatacccac atctattacg acagctcctt tttttatcca
     3361 agtgccgtgc acgaggtttg gtttgccgca agcgacaatt aagcaatcag cttgctgtac
     3421 atggcttatt aagtcctttg tgtgccgatg acatattgtt atagttgccc cagccattaa
     3481 caattctagt gccataggtt ttcccactac attcgaggca cctaccataa cagcatgctt
     3541 tcccttaaga tcataatgaa tatggtcaaa caatttcata attccataag gggtgcaggg
     3601 tcgtaacaga ggacgacctt gggtgagtct ccccgtattg taaggatgaa aaccatctac
     3661 atccttattc ggatttataa aatcgagaat ttgcttttcg cgaagatgct caggcaaagg
     3721 cagttgaact aatatgccat gaatatctat ttggtgattt aaggagtcca gtaagtttat
     3781 taattcttct gcagaagttt tttcgtgtaa attatatgaa aaagatttaa ttcccacatc
     3841 gaggcaagcg cgctttttat gatttacata aatagtggag gctgcatcct cacctacttg
     3901 tataactgca agaccaggag gggtatatcc ggcacgaacg aagcgttcta tttgaatttt
     3961 aagttcttgt ttaatagttt ccgcaatttt tttgccatcg ataatttgtg cactcatagt
     4021 ttatgtttaa aaagactgta gggtcattct ctcactaaac gcatctgtgc tccaagtgcg
     4081 ctcttcgtta aagataatgt ttgacgtatt tcctcgaaaa aagtattatt cgcgtgccca
     4141 gaaaaagtca aaggtaaaca acccacttat acaggtttgt tttagttttg aaacttatgc
     4201 gctaaaacgt gttttttatg tgcattttct ggtaaagtgc gtttcagttc ggggtatagc
     4261 gcagcctggt agcgcgtctg ctttgggagc agaatgtcgg gggttcaaat ccctctaccc
     4321 cgaccagtga gcttgcgcct gtagctcatt tggatagagc atcggccttc taagccgagg
     4381 gtagcaggtt cgaatcctgc caggcgcgcc aatgagcctt gagcaagttt tatggtgggc
     4441 gtagctcagt tggtagagcc ccggattgtg attccggccg tcgtgggttc aagtcccatc
     4501 gctcacccca tctgtcgggc cgttagctca gttggtagag cagctgactc ttaatcagcg
     4561 ggtcgtaggt tcgatcccta cacggcccac caattttgtt cgttaagtag gcaataaatt
     4621 agtttactac ttaattgaat ccaactgaat ttttaaaatt ttcggataat aaaagtcaaa
     4681 gaactaagtt ttggctatgc agttaaagtg ctatttgata acattatggt ggtaggttta
     4741 attttcttgt caggttgttt aaacctcatt atccttccca agtttgcgaa agtggcggac
     4801 ttggtagacg cgcaggattt aggttcctgt ggggtaacct gtgagagttc gattctctcc
     4861 tttcgcacca aaatcagagc aatgttctgg gataatcttt ttttttaaat aagcgaggat
     4921 taggtgatgg aagtctctgt tgaaaatact ggttcgttag aaagacgctt aacggtaagc
     4981 gtaccagagg cgcaaattaa tgaacaatgt cgcacaaaaa tggctgagct tgcaaaagat
     5041 gttaagctca aaggttttcg tcccggtaaa attcctccgc atattttgca acagcgattt
     5101 ggcaaggccg tgcgtgccga agtagtaggc gatatcatac aaaaaagttt agaaaatgcg
     5161 ctaaaagaaa aaaatttgtt gccagcaggc atgccgacta ttgagcaaat tcaagacgaa
     5221 tcaggaaaat cactagaata cactgctaaa tttgagattt ttcctgaaat aaaattaggc
     5281 gatctctccg aagtagaagt agaaaagaaa aaagtagata taaccgacga agacattcaa
     5341 aaaacgatta caaaaatgtg cgaaaactta ggcacatggg agtcagttac ccgcgcatcc
     5401 aaaatgggcg ataaattgat tatcgactta gaacgccgtt ggacaaccca agaagatgaa
     5461 ctacaaagct acgtaaaaat tgactttagc ttagacgaaa agaaatcttt gccagattta
     5521 gtcaatcaat tacttgataa aaatattgat gatgaaattg actgcacaat tacgtatccg
     5581 gatacctggc acgatgaaaa aataagaggt cgtgaagcaa cctttaaagt taaaattcat
     5641 gagattcttg aaaagaaacc gctaaccgtc gaagcattgg gagaaaaact caacctggat
     5701 aaagaagatc caaatatctt aaaagaaaaa attaaagaaa gaatggaaaa ggaagcttca
     5761 aaagtcattc aagatgaatt aaaagaaaaa gttctggaaa tattgcttga aaaaaatcct
     5821 atcgagaagc caaaaacttt aattgaacaa gaaaaaaagg cacttaaaga ggaaattgcg
     5881 ctttctg
//
LOCUS       DASTIB010000149         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_181240, whole genome shotgun sequence.
ACCESSION   DASTIB010000149 DASTIB010000000
VERSION     DASTIB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181240"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2219)
                     /gene="hrpA"
                     /locus_tag="VFP93_02575"
     CDS             complement(<1..2219)
                     /gene="hrpA"
                     /locus_tag="VFP93_02575"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET9843523.1"
                     /translation="MSWDKIIADCLYKDRGFFQKKLNEALSVSSHTHREHLLSKLRAR
                     IDHSISIRSAKFQQIPPIAYPDLPIMQAKAEILKAMEDNQVIIVAAETGSGKSTQIPK
                     FCLELQRGINGFIGHTQPRRLAARSIADRIAQELHQPVGKSVGFAVRFQDMVNPTTHI
                     KVMTDGILLNEIQYDKELLNYDTLIIDEAHERSLNIDLLLGYVRYLLEKGSHLKVIIT
                     SATIALEAFSQFFYHAPIIEVPGKLYPVETFYRSKEEQEDYFSHVKRVVEEAMTIDYG
                     DILIFLSGEGEIHEAQKLLTLQNYKNTEILPLYAKLNLDAQRKIFKTSSKRRIILATN
                     VAETSITVPNVRFVIDLGFARISRYNYRSKIQRLPIEPISQASCKQRQGRAGRTSAGV
                     CFRLYDESDFLTRPLFTEPEIKRSNLASVLLKMGQLQLGDLQNFPLLDPPEKSHIQDG
                     LKQLERLEAYKENKLTPLGHQMAKFPLDPKSSKILIKASQLNALAEMLIILSGLSIQD
                     PREYPKEFLEKATQIHSKFNHPKSQFLSYVNVWQHLHEVKKMHSQKQLRIYFHKNFLS
                     YVRFLEWQDIHQQLSAECKKMGLMLNEKAATYEEIHKALLSGFFENIGVFDAEKKCYM
                     GVRGVQFTVHKQALPIKPKWIMAAQFIETQKVYAACVAELDPTWILNVVPHLCKTHPG
                     EMFWDPKSGFVKNYESITFFGITIIARRAVLAKMHESSLQAIFIREALIEHALEEFP"
BASE COUNT          663 a          452 c          430 g          763 t
ORIGIN      
        1 aagggaaact cttctaatgc atgctcgatg agcgcctcac gtataaaaat tgcttgcaag
       61 ctactttcat gcatcttggc tagaactgca cgtcgcgcaa taatcgttat accaaaaaaa
      121 gttatgcttt cataattttt cacaaatccc gatttgggat cccaaaacat ctcacctgga
      181 tgggttttgc acagatgcgg aacaacgttt aaaatccaag tgggatcaag ctctgccaca
      241 caagcggcat aaactttttg cgtttcgata aattgtgccg ccataatcca cttcggttta
      301 atagggagtg cttgtttatg taccgtaaac tgtaccccac gtactcccat atagcatttt
      361 ttttctgcat caaaaacacc tatattttca aaaaaaccac tcaataaagc tttgtgtatc
      421 tcttcatagg ttgcggcttt ttcattcagc atcaggccca tttttttgca ttctgcgctc
      481 agttgttgat gaatgtcttg ccattcaaga aatcgaacgt aagataaaaa attcttatga
      541 aaataaatgc gtaattgttt ttgcgaatgc atttttttaa cttcgtgtaa gtgctgccat
      601 acattgacat agcttaaaaa ttgcgatttc ggatgattaa atttactatg tatttgtgtc
      661 gctttctcta aaaattcctt gggatactct cttggatctt gaatacttaa tccacttaaa
      721 ataataagca tttctgcaag tgcatttaat tgacttgcct ttattaagat tttgctcgat
      781 ttaggatcca aaggaaattt agccatttgg tgcccaagtg gggttagttt attttcttta
      841 taagcctcaa gccgctctaa ttgctttaac ccgtcttgaa tatggctctt ttcgggtgga
      901 tccagcagag gaaaattttg taaatcacca agctgaagtt ggcccatttt taataaaacg
      961 gatgccagat ttgagcgctt aatttcaggt tcggtgaata aaggtcgtgt taaaaaatcg
     1021 ctttcatcat ataatcgaaa gcatacaccc gcggatgttc ttcctgctct tccttgacgt
     1081 tgtttgcaag acgcttgcga aataggttca atgggtaaac gctgaatctt cgatcgataa
     1141 ttatatcgac ttatgcgcgc aaatccgaga tcgattacaa atcgtacgtt tggaacggta
     1201 atggatgttt ccgcgacatt ggtcgccaaa ataatgcgac gtttcgaact tgttttaaaa
     1261 atcttacgtt gtgcgtctaa attcaatttg gcatataaag gcaaaatttc tgtatttttg
     1321 tagttctgta atgtcaataa tttttgtgct tcatgaattt ctccttcccc tgataaaaat
     1381 attaaaatat caccgtagtc tattgtcatc gcttcttcaa cgactctttt tacatgcgaa
     1441 aagtaatctt cttgttcttc tttgctgcga taaaacgtct caaccggata taatttgccg
     1501 ggcacttcga taataggggc atgataaaaa aactgagaga aagcttctaa tgcaattgtt
     1561 gcggaagtta taataacttt taaatgtgaa cctttttcca gcagataccg tacataacct
     1621 aaaagtaaat caatatttaa actgcgttca tgtgcttcgt caataattaa cgtatcgtag
     1681 tttaacagtt ccttatcgta ttgaatttca tttaacaaaa taccatcggt catcactttg
     1741 atatgggtag tgggattcac catatcttga aaacgcacag caaaacccac actcttccct
     1801 acgggttggt gtaattcttg cgcgatacga tcggcaatag agcgcgctgc taatcggcgt
     1861 ggttgggtat gaccaataaa gccattgata cctctttgaa gttccaaaca gaacttagga
     1921 atctgcgtac tttttcccga tcccgtttct gctgccacaa taataacttg gttgtcctcc
     1981 attgctttca aaatctctgc ttttgcttgc atgataggga gatcgggata agcaatcggc
     2041 ggtatttgct gaaatttagc cgaacgaata gaaatcgaat gatctatgcg tgcccgaagt
     2101 ttggaaagca aatgttctcg atgggtatgg ctagagacgc ttaaagcctc gtttaatttt
     2161 ttttgaaaaa agccacgatc tttatataag cagtctgcaa ttattttatc ccaagacatt
     2221 taaaaaccta gccaagcttc tcgtaacatt ttttgggagg gcgttaaaat atccatgggg
     2281 tttaattgaa tactttgctt gggtgctt
//
LOCUS       DASTIB010000150         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_283795, whole genome shotgun sequence.
ACCESSION   DASTIB010000150 DASTIB010000000
VERSION     DASTIB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283795"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /gene="rluD"
                     /locus_tag="VFP93_02580"
     CDS             <1..961
                     /gene="rluD"
                     /locus_tag="VFP93_02580"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HET9843524.1"
                     /translation="KEVLEGKVVVPEQMKNARLDKVLSELFPSYSRTKLVEWLDAGFV
                     RVNDKIKLDRKTKMQGGECIVLCVELQNDTTHIPQALSLDIVYEDDHILIIQKKAGQI
                     VHPGAGNRDNTLLNGLIYARPELGSLPRAGIVHRLDKDTSGLMVVAKTLSAHHGLVKS
                     LQMRTIDRHYYALCQGTLISGGTIHTSMGRHPTNRIKMAVTTQGKEAITHYRVEKKFR
                     AHTLIDVKLETGRTHQIRVHMAHLGFPLVGDPLYGRLKFPKKCSHACKEALANFKRQA
                     LHAYRLHFKHPITQEVLDFKIPLPEDFAHLLEIVKNDDSQQLD"
     gene            936..1676
                     /gene="pgeF"
                     /locus_tag="VFP93_02585"
     CDS             936..1676
                     /gene="pgeF"
                     /locus_tag="VFP93_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET9843525.1"
                     /translation="MMIPNNWIKPDWPAPLNIQAFTTTRNLTVHDKVLDFNLGLHATQ
                     YPASVKSNRALLDKIVGNPIHWLNQIHSAEIAQLPNADAVLNADGAFSITKNTICAVM
                     SADCMPVLICDTSGFLVCALHCGWRGFAQNIIQNAVKKIRIHTNNPLMAWLGPAIGVK
                     FYEVGEEIRELFVKYFFESGVAFTSHAVPGKYWLDLTQLAKLLLHREGIESIFEEAYC
                     TYQNAQDFYSARRDKNTGRFATVIWKTE"
     gene            1740..>3451
                     /locus_tag="VFP93_02590"
     CDS             1740..>3451
                     /locus_tag="VFP93_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9843526.1"
                     /translation="MRIDKFTNKFQAALSDAQSIAVGRSHPTIEPIHVLAAFLNQDAS
                     LVTNIFQNAGVSVSNLRTKVQSALEELPKVGEQTGEIRISDELNRLLILTDKFAQDRK
                     DAFISSELFLLALLEDRGHAGKILKACGVTKSNLDKAISAIRGGETVKDPNAEEHRQA
                     LDRYTIDLTKRAEQGDLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGKRVLVLDLGALIAGAKFRGEFEERLKAVLKDLSKQEGEIILF
                     IDEIHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KILIDQPNVEDTIAILRGLKERYEVHHGVEITDPAIVSAATLSARYITDRMLPDKAID
                     LIDEAASRIRMAIDSKPEPLDALERRLIKYKIEREALKKENDDLSKQRLKELEQNITL
                     VEKQYSELEEVWKAEKAAVLGAKNIKEELEKARIALDAARRANDLAKMSELQYGRIPE
                     LEKQLLVASAMETQEQTLLKNKVGEEEIAEVVSKWTGIPVSRMLEGERDKLLRMEQEL
                     GKRVV"
BASE COUNT         1122 a          655 c          778 g          896 t
ORIGIN      
        1 gaaagaagta cttgaaggaa aagtggtagt accagagcaa atgaaaaatg ctcggttaga
       61 taaagtactt tccgaattat ttcccagtta ttcgcgaaca aaattagttg aatggcttga
      121 tgcgggtttt gtgagagtta atgataaaat aaagcttgat cgcaaaacaa aaatgcaagg
      181 cggtgaatgt atagtgcttt gcgttgagct acaaaatgac acaacgcata taccacaagc
      241 tctgagtctc gatattgtat acgaagatga tcatatcctt attattcaaa aaaaagccgg
      301 tcaaattgtg catcctggag cgggtaatcg cgataatacc ttattaaatg gcttaattta
      361 cgccagacct gaactgggtt ctttaccgag agccggaatt gtgcaccgat tagataagga
      421 taccagtggt ttaatggtgg tggcaaaaac attgagtgca catcatggtt tagttaaaag
      481 tttacaaatg cgcaccatag atcggcatta ttatgcttta tgccagggca cattgattag
      541 tggtggcact attcatacct ctatgggtag gcacccaact aatcgtataa aaatggcggt
      601 aaccacacag ggcaaagaag cgataaccca ttatcgcgta gagaaaaaat ttagagccca
      661 tacgttaatt gatgtaaaac tagaaacagg acgcacccac caaatccgag ttcatatggc
      721 acacctaggc tttcccttgg tcggtgatcc gctttatggt aggttaaaat ttccaaaaaa
      781 atgttcgcac gcatgtaaag aagctttggc caatttcaag cgccaggctt tgcatgccta
      841 tcgattgcac ttcaagcatc ctatcaccca agaagtattg gactttaaaa taccattgcc
      901 ggaagatttc gcgcatttac tggaaatagt aaaaaatgat gattcccaac aactggatta
      961 aacctgactg gcccgcgccc ttaaatattc aagcgtttac cacaacccgc aatttaacag
     1021 tgcatgataa agtattagat tttaatctag gcctgcatgc tacacaatac cccgcaagtg
     1081 taaaaagcaa tcgtgcatta ttggataaaa tagtagggaa tcctattcat tggctgaacc
     1141 aaatacacag cgctgaaata gcccaattgc caaatgcgga tgcagttctc aatgcagatg
     1201 gtgcgttttc cataacaaaa aatacaatat gtgcagtcat gagtgcggat tgtatgcccg
     1261 ttttgatctg tgacacctcg ggttttttgg tttgtgcgtt gcactgcggt tggcgcgggt
     1321 ttgcccaaaa tattatacaa aatgctgtaa aaaaaattag gatacataca aataatccct
     1381 taatggcgtg gctgggaccg gcaatcggtg ttaaatttta tgaggtaggt gaggagattc
     1441 gggagctttt tgtaaaatat ttctttgagt ctggcgttgc atttacatcg catgctgtgc
     1501 ctggaaaata ttggcttgat ctaacacaac ttgcaaaatt gctgttacat agagaaggta
     1561 tcgaaagtat ttttgaagaa gcatactgta cgtatcaaaa tgcacaagat ttttattccg
     1621 cacggcgtga taaaaacacg ggtcggtttg caacagtaat atggaaaacc gaataacgct
     1681 ttaaaataag caattgcgtc cccataatat agttaattca ttttattgat aaggttgcta
     1741 tgcgcattga taaattcacg aacaagtttc aagctgcgct gtcggatgcg caatctattg
     1801 ccgtgggaag atcgcatcca accattgagc ccatacacgt attagctgct tttttaaatc
     1861 aagatgcatc tctcgtcacg aatatttttc aaaacgcagg tgtgagtgtt agcaacttgc
     1921 gaaccaaagt ccaaagtgct ttagaagaat tacctaaagt aggtgagcaa accggcgaaa
     1981 tacgaatttc agatgaattg aatcgattac tcattttaac cgataaattt gcgcaagatc
     2041 gaaaagacgc ttttatctct agtgaacttt ttcttctggc cctcctagaa gacagagggc
     2101 atgcaggaaa aattcttaaa gcatgtggcg ttaccaaaag taatctggat aaagccatat
     2161 ctgctattcg aggcggagaa acggtaaaag atccgaacgc cgaagaacat cgccaagcat
     2221 tggatcgcta caccattgac ttaaccaaac gcgccgaaca aggtgactta gaccctgtta
     2281 ttggtagaga tgaagaaatt cggcgcacaa ttcaagtttt acaaagaaga accaaaaata
     2341 accccgtgtt gattggcgag cctggtgttg gaaaaaccgc cattgtcgag ggcttagccc
     2401 aacgtattat aaacggtgaa gtgccagaag ggttaaaagg caaacgcgtt ttagtgcttg
     2461 atttaggcgc gctgattgcc ggagctaaat ttcgtggcga atttgaagaa agactcaaag
     2521 cggtgttgaa agatctttcg aaacaagaag gggaaatcat tttatttata gatgaaattc
     2581 atacgatggt aggtgcaggc aaggccgaag gggcgatgga tgcggggaat atgttgaagc
     2641 ctgcgttggc gcgcggtgag ctgcattgtg tgggggcaac aaccctcgat gaatatcgtc
     2701 aatatattga aaaagacgct gctttagaac gtagatttca aaaaatttta attgatcaac
     2761 ccaatgtgga agacaccatt gcaattttac gcggtttaaa agagcgctat gaggtacatc
     2821 atggtgtgga aataaccgat ccggctattg tttcagcggc aaccttatct gcgcgctata
     2881 ttaccgatcg catgttaccc gataaagcaa tcgatctcat tgatgaagca gccagtcgaa
     2941 ttcgtatggc aatcgattca aagcccgaac ctttagatgc gctagaacga cgtcttatta
     3001 aatataaaat tgagcgcgaa gcacttaaaa aagaaaacga cgatctttct aaacaacgtt
     3061 taaaagaatt agaacaaaat attacgctcg ttgaaaagca atattcagag cttgaagaag
     3121 tttggaaagc agaaaaagca gcggtgctag gcgctaaaaa catcaaagaa gaacttgaaa
     3181 aagctcgtat cgctttagac gctgcacgac gcgccaatga tttggccaaa atgtcggaac
     3241 tgcaatacgg gaggatccca gaattagaaa agcaattatt ggtggcttcc gccatggaaa
     3301 cccaagagca aactttgtta aagaataaag taggcgaaga agaaattgct gaagtggtct
     3361 ctaaatggac aggtattcct gtaagccgta tgctcgaagg cgagcgcgat aaattattgc
     3421 gcatggaaca agaacttggc aaacgcgtcg t
//
LOCUS       DASTIB010000151         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_880536, whole genome shotgun sequence.
ACCESSION   DASTIB010000151 DASTIB010000000
VERSION     DASTIB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880536"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..196)
                     /locus_tag="VFP93_02595"
     CDS             complement(29..196)
                     /locus_tag="VFP93_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET9843527.1"
                     /translation="MKKYMCLLCGVIYDEALGWPEEGLAPGTLWADVPLNWRCPECGA
                     TKDDFEMVETA"
     gene            320..1606
                     /gene="hemL"
                     /locus_tag="VFP93_02600"
     CDS             320..1606
                     /gene="hemL"
                     /locus_tag="VFP93_02600"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET9843528.1"
                     /translation="MSKSVESFLRAQKSIPGGVNSPVRAFQSVGGTPIFFTKGKGAYL
                     YDIDGKEYIDYMASWGPLILGHSNPHVVEEIQLALENGLSFGAPTERETILAEKILES
                     MPAIEKIRMVNSGTEATMTAIRLSRAHTNRAKIIKFEGCYHGHSDGLLVKAGSGALTL
                     GSPSSPGVPTEIASLTLNLPFNDITSLHNAFAEFGEDIACVIVEPVAGNMGCVLPTLE
                     FLRALRQSCTNYGAVLIFDEVITGFRVSLSGAQGYYEIIPDLTTLGKIIGGGMPVGAV
                     GGKNAIMCNLAPEGKVYQAGTLSGNPVAMTAGFATLTQCSQPNFYSYLETQTQKIVSG
                     FQEMAQKHSIPLVVNSVPGMFGLFFTEQGSVNSFENAVNCDIELFKQFYQQALKEGLY
                     FGPSAFESAFVSVCHNDTILELTFQKIDKIFAKLKV"
     gene            1617..3095
                     /locus_tag="VFP93_02605"
     CDS             1617..3095
                     /locus_tag="VFP93_02605"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9843529.1"
                     /translation="MDEIIQALQDSSIYNHEVYQFSIVETHISWILLTGLFAYKIKKP
                     VNLGFVDYSTLAKRKYFCELELVLNKKLAPNLYLDVFPLARKNNKIIFDSKSPPVEYV
                     IKMHQFDESQMLNKLCETEGLSKGIVRSLATQIADFHKNTEIAPKGSIFGTHKNVFQM
                     MLDNFSLLSTCNLDAAMEQKIKLIEQWTHETYTSLHSVLEQRKEIGHIKACHGDLHLG
                     NIVLIQGNPTIFDCIEFNDNLRFTDVIADIAFLAMDLDHKSYHKLSNFFVNHYLELTQ
                     DYPGATLLSFYKAYRAMVRAKICAIQFSSLEKKDTLLADFTRYLDIAIAYQAKPGSEL
                     IITHGPSGSGKSFYTEALLETNTLRLSADVFRKQLFHLKPYALSPLSLRSNLYSEKTT
                     KEVYRLLYEQADYLLSRGFRVIVDATFLKQWQRQQFLQLSVSHNIPFKILDFPMNMEL
                     LKSRIVNKADPNASDADMEVLRKQLNALEPLTEYEKSFVEVI"
     gene            3104..3334
                     /locus_tag="VFP93_02610"
     CDS             3104..3334
                     /locus_tag="VFP93_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843530.1"
                     /translation="MQQLVSEDLRHIHGANIVTPTLVAMIVGCGSGILGIHLLQTYEQ
                     DHELSSLHRYLLEGSIGAAIGILGGWGFILSA"
BASE COUNT         1030 a          617 c          675 g         1061 t
ORIGIN      
        1 ttgtattgct agttgtttta aagcctttct aggcggtctc aaccatttca aagtcatctt
       61 tggttgctcc gcattcgggg caacgccaat ttaatggcac atctgcccat aacgttcctg
      121 gtgctaaccc ttcttcaggc caacctaacg cttcatcgta gattacaccg cacaataaac
      181 acatatattt tttcataagt tttatgtcca acaaaattta tgggcatcat tctctcataa
      241 tcaaatgagg aaaaaaagtg tatgcaaata caaagaattt tttttggttt ataattcaat
      301 tttttacaag gaatggtcca tgtcaaaatc tgttgaaagc tttttacgcg ctcaaaaaag
      361 tataccaggt ggtgtgaatt caccggtgag ggctttccaa tcggtagggg gaactcccat
      421 tttctttaca aaaggtaaag gtgcatacct ttatgatatc gatggtaaag aatatattga
      481 ctatatggct tcttgggggc ctttgatttt aggtcatagt aatccacatg ttgttgagga
      541 aattcaactg gccttagaga acggattaag ctttggtgcg cctacggaac gtgaaacgat
      601 tttagcggaa aaaattctcg aatctatgcc agcaatagaa aaaattcgca tggtgaattc
      661 tggaacggaa gcgacaatga cagccattcg attgagtcga gcgcatacca atcgggcaaa
      721 aattattaaa tttgaaggtt gttatcatgg tcatagcgat ggtttactcg taaaagctgg
      781 atcgggtgct ttaacactag ggagcccaag ctcacctggc gttccaacag aaattgcaag
      841 tcttacgcta aatttgccat ttaatgatat aacaagttta cacaacgcat ttgccgagtt
      901 tggtgaagat attgcttgcg ttatcgtgga acctgttgca ggtaatatgg gttgtgtttt
      961 acccacgctt gaatttcttc gcgcgttaag acaaagttgt accaattatg gtgcggtatt
     1021 aatttttgac gaggttatta cggggttcag agtgtcgctt tcaggcgctc aagggtatta
     1081 tgaaataatt cccgacttaa ctacattggg caaaataatt ggtggaggta tgcccgtagg
     1141 tgcggtgggt ggaaaaaacg caataatgtg taatcttgca ccagaaggga aagtatatca
     1201 agccggaact ttgtctggca accctgttgc tatgacagct ggctttgcca ctttgaccca
     1261 atgctcacaa ccgaattttt attcttatct tgaaacgcaa acgcaaaaaa ttgtgagcgg
     1321 ttttcaagaa atggcgcaaa aacatagtat tccattagta gttaatagcg ttccaggtat
     1381 gttcggtctc ttttttactg aacaaggatc cgtaaatagc tttgagaacg cggttaattg
     1441 cgatattgaa ctgtttaaac aattttatca acaagcgctg aaagaaggct tgtactttgg
     1501 accttctgca tttgagagcg cttttgtgtc agtttgtcac aatgatacca tccttgaatt
     1561 aaccttccag aaaattgata aaatctttgc aaagttgaaa gtttaaggac tttttcatgg
     1621 atgaaataat tcaagctttg caagattcca gtatttataa tcatgaagtt tatcaatttt
     1681 ctattgttga aacccatatt tcttggatat tacttaccgg cttatttgct tataaaataa
     1741 aaaaacccgt gaacctcggt tttgtggatt attcaacttt agcgaaacga aaatactttt
     1801 gtgagctcga gttggtgtta aataaaaaat tggcgcccaa tctttatctt gatgtatttc
     1861 cgcttgcaag aaaaaataac aaaattattt tcgattctaa gtcgcctcct gtcgagtacg
     1921 tcataaaaat gcatcaattt gatgaatcac aaatgttgaa taaactgtgt gaaacggaag
     1981 gtttatccaa aggtattgta cgatctttag ctacgcaaat tgcagatttt cataaaaata
     2041 cggaaattgc tccgaagggt agtatcttcg ggactcacaa aaatgtattt caaatgatgt
     2101 tggataattt ttccctattg tctacatgca atttagacgc agctatggaa cagaagataa
     2161 aacttattga acaatggacg catgaaacgt atacaagcct gcattcagtt ttagaacagc
     2221 gtaaagaaat cggtcacata aaagcatgcc acggtgatct ccatttaggt aatattgtac
     2281 tcatacaagg aaaccctaca atctttgatt gtatagaatt taacgacaat ttacgtttta
     2341 ccgatgtaat cgcagatatc gcttttctgg ctatggattt ggatcacaaa tcctatcata
     2401 aactcagcaa tttttttgta aatcattatt tagaattaac gcaagattac ccaggagcta
     2461 ctttgctttc gttctacaaa gcgtatcgtg ctatggttcg cgctaagatt tgcgccatcc
     2521 aattttcatc cttggaaaaa aaggatacct tgctcgcgga ctttacgcgc tatctagaca
     2581 tagccattgc ttaccaagcg aagccaggaa gcgaattaat tattacccat ggaccttcag
     2641 gatcgggtaa aagcttttat acggaagctt tattggaaac caatacgctt cgtttaagtg
     2701 cggatgtatt tagaaaacag ctttttcact taaagcctta tgcactaagt ccgttatccc
     2761 tgcgttctaa tctttacagt gaaaaaacta ccaaagaagt ctatcgtttg ctctatgaac
     2821 aagccgatta tttgttatca cgcggttttc gcgttatcgt cgacgctact tttttaaaac
     2881 agtggcagcg acaacagttc ttacaactaa gcgtgtcgca taatatacct tttaagatcc
     2941 tcgatttccc tatgaatatg gaactactaa aatcacgaat tgttaataaa gcagatccga
     3001 atgcgagtga tgctgatatg gaagttttaa gaaagcaatt aaatgcttta gagccattaa
     3061 ccgagtatga aaaaagtttt gttgaagtca tctaggagaa aaaatgcaac agcttgtaag
     3121 cgaagattta cgccatattc atggtgctaa tattgtaacc cctacgttag tcgctatgat
     3181 tgttgggtgt ggtagtggga tcttaggaat acatttactc caaacctatg aacaagacca
     3241 tgaactaagt agtttgcatc gatatcttct ggaaggaagc attggcgctg ctattggaat
     3301 attaggcggt tggggtttta ttctctctgc ttaataatgg tattgtgtac catacgttct
     3361 ttgtggtaca caaacctcaa tct
//
LOCUS       DASTIB010000152         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1479554, whole genome shotgun sequence.
ACCESSION   DASTIB010000152 DASTIB010000000
VERSION     DASTIB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479554"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFP93_02615"
     CDS             <1..602
                     /locus_tag="VFP93_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HET9843531.1"
                     /translation="ILGITKVSSLSFFSDKALEENFNVYIDVDSHTLCLRLPHDRAFV
                     IVDTQIAIKYWQKLATTAIITNEVVWDWFDIQAGIPQLTASTTEFFLPHYLNLPQLGG
                     VNFEKGCYLGQEIIARMQYRGHIKKHLYRAKIATKHAVEPGQEIHNETGQVVGNIVKA
                     VVEENQVFCLISAQDESITQSLFLSGTQDFFYEIMDAAN"
     gene            complement(753..>2084)
                     /gene="lysS"
                     /locus_tag="VFP93_02620"
     CDS             complement(753..>2084)
                     /gene="lysS"
                     /locus_tag="VFP93_02620"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9843532.1"
                     /translation="NKNVQVKCAGRIMTRRNMGKASFVHLKDMTGLIQVYIKQDLLPD
                     HVYESFKHWDLGDIVAIEGVVFKTKTGELSIRASNIKLLSKALRPLPDKYHGLHDQEV
                     CYRQRYLDLMVNQKTQKVFQIRSKLIQSLRDFLLERQFLEVETPMMQVIPGGAAAKPF
                     ETYHNALDMPLFLRIAPELYLKRLVVGGFEQVFEINRNFRNEGLSTRHNPEFTMVEFY
                     WAYADYQDLMTLTEEMMRYLAMQVLGTYEISYQENIIDFSKQFPRLTVQEAILQFNSK
                     LTKADLGDLTSMIAYAKTLEIPLKPEYGLGKVLTEIFEKTAEPNLQGPLFITEYPAEV
                     SPLARRKDSDPFVTDRFEFFIGGREIANGFSELNDPDDQRERFLKQIEAAKAGDEEAM
                     RFDEDYLTALEYGMPPTAGEGIGIDRLAMVFADCASIRDVILFPHMRPTDK"
BASE COUNT          631 a          414 c          414 g          625 t
ORIGIN      
        1 cgattttagg aattacaaaa gtgtcaagtc tgtctttttt tagcgataag gctttagaag
       61 aaaattttaa cgtctatatt gatgtcgact cacacacact gtgtttgaga ttacctcacg
      121 atagagcatt tgttattgtg gatacgcaaa tagccataaa atattggcaa aaacttgcaa
      181 caacagcgat tatcacaaac gaagtagttt gggattggtt tgatattcaa gccggaatac
      241 cacaattaac cgcttccacc accgaatttt tcttacccca ttatctaaat ttgccacaac
      301 taggtggggt aaactttgaa aaaggatgct acttaggcca agaaatcatt gcacgaatgc
      361 aatatcgtgg ccatataaag aagcatctct atcgtgcaaa aattgcaact aaacatgctg
      421 ttgaaccggg tcaagaaata cacaatgaaa cggggcaagt tgtaggaaat attgtgaagg
      481 ctgtcgtcga agagaatcaa gtattttgtc ttattagcgc tcaagacgaa agcataactc
      541 agtccttgtt tctaagtggc actcaagatt ttttttatga aataatggat gccgcgaact
      601 aaagtcgcgg cccgtaggca gcaaaatctc gttgtagcga agcgtaggtg gatgggccgc
      661 gaactaaagt cgcggcacgt aggcagcaaa atcgcggcgt agtgaagcgt aggtggatgg
      721 atccgcgaac taaagtcgcg gctggggttt cattatttat cggttggtcg catatgtgga
      781 aataaaatca cgtcgcgaat ggatgcacaa tccgcaaaaa ccatggctaa tctatcaata
      841 ccaatgccct ctcccgcggt tggaggcata ccatactcca aagcagtaag ataatcttca
      901 tcaaagcgca ttgcctcttc gtcaccggct ttggctgctt ctatttgttt taagaaacgc
      961 tcgcgttgat catcgggatc atttaattca gagaaaccat tcgcaatttc acgccctccg
     1021 ataaaaaatt cgaatctatc ggtaacaaaa ggatcagaat ctttacgacg tgcaagagga
     1081 gaaacctctg ctggatattc ggttataaat aaaggacctt gtagattagg ttcggcagtt
     1141 ttttcaaata tttcggtaag taccttgcct aagccgtatt ccggtttaag aggaatttct
     1201 aacgttttcg catacgcaat catgcttgtg agatcaccta gatctgcttt agtaagtttc
     1261 gaattaaatt gtaaaattgc ttcttgaacg gttaacctcg gaaattgctt tgaaaaatcg
     1321 atgatgtttt cttggtaaga aatctcataa gtgcccaata cttgcattgc taaatagcgc
     1381 atcatttctt ctgtaagcgt cattaaatcc tgataatcgg cataagccca ataaaattct
     1441 accatcgtaa attcagggtt atgccgagtc gataaacctt cattgcggaa gtttctgttt
     1501 atttcaaaaa cttgttcaaa gccccccacc accaatcgtt ttaggtaaag ctctggtgca
     1561 attcttaaaa ataatggcat atctaatgca ttatggtagg tttcgaaagg ttttgccgca
     1621 gcacctcccg ggatcacttg catcatgggt gtttcgactt ctaaaaattg tcgctctaat
     1681 agaaaatctc gcaacgattg aattaacttg gagcgtattt gaaaaacttt ttgtgttttt
     1741 tgatttacca ttaaatcaag atagcgttga cgatagcaaa cttcttgatc atgcaaccca
     1801 tgatatttat caggcagagg tcgcagtgct ttggataata attttatgtt acttgcgcgt
     1861 atcgataatt cgcccgtttt ggttttaaat acaacgcctt cgatagcgac aatatcacct
     1921 aaatcccaat gtttaaatga ctcataaaca tgatcaggga gcaaatcttg ctttatataa
     1981 acttgaataa gtcctgtcat atctttcaaa tgaacaaaac ttgctttacc catattgcgt
     2041 ctggtcataa ttcggccagc acattttact tgcacgtttt tatt
//
LOCUS       DASTIB010000153         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_251316, whole genome shotgun sequence.
ACCESSION   DASTIB010000153 DASTIB010000000
VERSION     DASTIB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251316"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..543
                     /locus_tag="VFP93_02625"
     CDS             91..543
                     /locus_tag="VFP93_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9843533.1"
                     /translation="MSMIRYQPNDLFEKLQRELLNFTPWNRELANEDRSTVATSEWIP
                     RVDIIEEDHQFLVRADIPGVDPKDIEISMNGNTLTVRGERKSETKEESKKFTRVERLY
                     GTFYRQFTLPDSVVGEQITAKSKHGVLEIAIPKEEKAKPRTINVAIEE"
     gene            663..1757
                     /locus_tag="VFP93_02630"
     CDS             663..1757
                     /locus_tag="VFP93_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843534.1"
                     /translation="MSFEVHVISSNKSPQTFEINNLSLEAVVAFLYDPDKSVGRLTAD
                     DVANIIEANKNQASIKIDAITNQVDKEERVLGLTIDGAWDNELFIVKKLTINRDRLAL
                     GKVTHKGNSASFASDVDLRNIFKQLQHFDIELSQSDEDSDSSMEEIAINEPPKNLQST
                     PSSPKNAPNVNAEKVNEVAPEEKNVPKSSEPESKPNPNPDSIADPKKEEMVAKTLKEK
                     LAQKNDNASSLFKVPSLLAAVSFVGRWLLLGLTTTVALVGAAATFVVTGILNLARHYL
                     KRNGYQKVFPEENIESVEGSIAKLSDEDFEVFKHGVDAKNEILNQFKSCFRPKDWVHA
                     DKFYAGYTFKEKFPEVNEEDLNSLRKPQAK"
     gene            1947..>2453
                     /locus_tag="VFP93_02635"
     CDS             1947..>2453
                     /locus_tag="VFP93_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843535.1"
                     /translation="MKFVVQLNKTGKAPAKRIEKDLELQDTYAVLANLWNAGLTHISA
                     ENLIKSEQMEAIIQDLIERNVGEEIINFKGKIKNAPFHLRLEGTWDEDGSYELTKIIF
                     YENHYTSKFDLNSVKQKKKAGTAQEVLNTFESTLENDHVLNQEPAPINNASNANEERR
                     FVLNNENQD"
BASE COUNT          874 a          426 c          462 g          691 t
ORIGIN      
        1 cacgcgaaca aatcctcttg aaattttata aactatcccc atctcaatag taacgatagt
       61 taaacgtagt taatcaaatg ggagggtatt atgagcatga ttcgttatca acctaatgat
      121 ttattcgaaa aattacaaag agaattactt aattttacac cttggaatcg tgaattagcg
      181 aacgaagata ggtctaccgt tgcaaccagt gaatggatcc cgcgcgtaga tataattgag
      241 gaggatcacc aatttttggt acgtgcggat attccaggag tggatcctaa agatattgaa
      301 atttctatga atggaaacac gttaaccgtt cgaggcgaga gaaaaagcga aactaaagaa
      361 gaatccaaaa aatttacgcg ggttgaaaga ctctatggta ctttttatcg ccaatttacc
      421 ttaccagata gcgtggtggg tgaacaaatt accgccaaat ccaaacatgg tgtgttagaa
      481 attgcgattc ctaaagagga aaaggcaaaa cctcgtacga taaatgtcgc aatcgaagaa
      541 taaagagtgc cgtattttat acggcatttt ttatgaaagc gtgtaattgc ctttgtttca
      601 aaaggtacct ataatttgcc tggaatttct acatgtagct agaatataag gtaggtatct
      661 caatgagttt tgaagttcac gttatttctt ctaataagtc tccccaaact ttcgaaatta
      721 ataatttgtc cctagaagcc gtggtagctt ttctctatga cccagataaa tctgtaggtc
      781 gattgacggc cgatgatgtt gcaaatatta tcgaagcaaa caaaaatcaa gcttctatca
      841 aaatagatgc tattaccaat caggttgaca aagaagaacg tgttctaggc ctgacgattg
      901 acggtgcatg ggataatgaa ctttttatag ttaaaaaatt aaccatcaac cgtgacagac
      961 ttgctcttgg taaagtaacc cataagggta atagtgcaag ttttgctagt gacgtagact
     1021 taagaaatat ttttaaacaa ttacagcatt ttgatataga actgtctcaa agtgatgaag
     1081 attctgattc cagtatggaa gaaattgcaa ttaatgaacc accaaaaaat cttcaatcaa
     1141 caccctcgag tcccaaaaac gctcccaatg taaatgctga aaaagttaac gaggtagcac
     1201 cagaagaaaa aaatgtgcca aaatctagcg aaccagagtc aaaacccaat cctaatcctg
     1261 attccatcgc tgatccaaaa aaagaagaaa tggtagcgaa aaccttaaaa gaaaaattag
     1321 cacaaaaaaa tgataatgct agttctctat ttaaagtacc gagtttgttg gctgcagtat
     1381 cctttgtagg acgctggttg ctgttagggc ttaccacaac cgttgctttg gttggtgctg
     1441 ccgctacttt tgtagttaca ggtattctta acctcgctcg acattattta aaacgtaacg
     1501 gttaccaaaa agtatttcca gaggaaaata tagaatccgt tgaaggctcc attgcaaaat
     1561 taagcgatga agatttcgaa gtgtttaaac atggggtcga tgccaaaaat gagatattaa
     1621 atcaatttaa atcttgtttt agaccaaaag actgggtaca tgcagacaaa ttttatgcag
     1681 gctatacctt caaggaaaaa ttcccggaag taaacgaaga agatttaaat tctctaagaa
     1741 aaccgcaagc aaaataatat atacgagcaa cagggaagtt actcgcctca acatcttgca
     1801 ctatattgtt aacttacttt tccatgtgaa aatcattatt tttataagtt aattgatttt
     1861 aatttgctaa ataacttgcc caaagctaat ccttctccta ttatttctcc ttgcccagtt
     1921 tctaactaaa tattttggag atgaaaatga agtttgttgt tcaacttaac aaaacaggta
     1981 aagcaccggc aaagcgtatt gaaaaagatc tggaactcca ggacacgtat gcggtattag
     2041 ctaacttatg gaacgctggt ttaacccata tttctgctga aaatctgatt aaatcagaac
     2101 aaatggaagc aattattcaa gatttaattg agcgtaatgt tggtgaagag attattaact
     2161 ttaaggggaa aataaaaaat gcaccttttc acctacgctt agagggaact tgggatgaag
     2221 atggctctta tgaacttacc aaaattattt tttatgaaaa ccattacacg agtaagtttg
     2281 atctgaacag cgtcaaacaa aagaaaaagg caggtaccgc tcaagaagta ttgaatactt
     2341 ttgaaagtac tttggaaaac gatcacgttt taaaccaaga acccgcaccg atcaataacg
     2401 catcgaatgc gaacgaagaa agaagatttg tgttaaacaa tgagaatcaa gac
//
LOCUS       DASTIB010000154         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_419037, whole genome shotgun sequence.
ACCESSION   DASTIB010000154 DASTIB010000000
VERSION     DASTIB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419037"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFP93_02640"
     CDS             complement(<1..966)
                     /locus_tag="VFP93_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843536.1"
                     /translation="MPLTLEHLHSLGAERPNDYLKFDENPDSINQTLQWMKDLSSADF
                     TFAVGEKRINEQFKIMNAQLKGLAATASKNISIQKNFYFSQAVGYLFKNLRKGLNNLL
                     QGRKKRKWLTILGSMGLGAGVGAIIGTMVFPLLGTAVGGALGSAIGGISAAVAGPIGL
                     SFLGGAFGTWLGRKFAKKFAPSKSVYNISEKAKSSLKLMLNLEEDELVKLHAYLINRA
                     NSVKDPTLKKALRLLDNTIFKNKAPTGVVRLTDFLKSELAELEQLDAPNSQHEADKQF
                     IQKILAKLPNHLNPLQSQTALQDKNIQSDNDSKQPNANLVQDKKLK"
     gene            1159..1953
                     /locus_tag="VFP93_02645"
     CDS             1159..1953
                     /locus_tag="VFP93_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9843537.1"
                     /translation="MGIPVPRTHVCIQSSIDVRQIAAPLFQYTNIHYFCYGRNYPNGG
                     TFTLHTDVNFYDSWFIQEFPLVLSFLKEGLHLWDDCQNADLIRVRLNDFNYGNGLYIV
                     RHFPEYSEIFGFAPAREQNISDLYLNNLDVLNRFAMYFKDKAYRIIERAEKDLIFVPK
                     TMLIEKEELASNNNLELLRNTTKIEMYALSPTLNLELTQREHESFKGFLQGQTAAQIA
                     SSLNLSPKSVETFLLKVKKKFQCSTRSDLLKRAWDLGILQSNGWFD"
     gene            complement(1954..>2075)
                     /locus_tag="VFP93_02650"
     CDS             complement(1954..>2075)
                     /locus_tag="VFP93_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET9843538.1"
                     /translation="IAHKTSSREAFAQGALVAARWITNKSKGLYDMQDVLGLK"
BASE COUNT          613 a          384 c          367 g          711 t
ORIGIN      
        1 tttgagcttt ttatcttgta ccagattcgc attgggttgt tttgaatcat tatcggattg
       61 aatattttta tcctgcaaag ccgtctgtga ttgtaatgga ttcaagtggt taggtaattt
      121 tgctaaaatt ttttgaataa attgcttatc tgcctcatgt tgactatttg gagcatctag
      181 ttgctccaat tcagctaatt cactttttaa aaaatcggtc aatcgtacga cacccgtggg
      241 tgctttattt ttgaaaattg tattgtctaa aagacgaagc gcttttttaa gtgtaggatc
      301 tttaacacta ttggctcgat tgataagata cgcatgaagt tttacaagtt catcctcttc
      361 taaattcagc attaacttta gtgacgattt ggctttttca gaaatgttgt agacggattt
      421 tgagggtgcg aatttttttg caaattttct accgagccaa gttccaaagg cgcctcctaa
      481 aaagcttaat ccaatcggcc ctgccacagc tgcactgatt ccccctatgg ctgaaccaag
      541 tgccccaccc acagcagtcc ctaggagggg aaaaaccata gtacctataa tcgctcccac
      601 tccagcacct aatcccatag agcctagaat ggttaaccat tttctttttt tacgtccctg
      661 taataaattg ttcaaacctt tgcgcaaatt cttaaaaaga tatcctactg cttggctaaa
      721 atagaagttt ttttggatgg agatattttt tgatgcggtg gccgcaagac cttttaattg
      781 cgcattcatt attttgaatt gttcattaat acgtttttca cctaccgcga aagtaaaatc
      841 tgcagaactt aaatctttca tccattgcaa cgtttgattg atagagtcag gattttcatc
      901 aaactttaaa taatcattag gtcgttctgc gcccagactg tggagatgtt caagcgttaa
      961 aggcataccc atccagatca ggttttttca gtttgactta agaattgcat ataaaattcc
     1021 ttaattttta tttttttacg aaaaaataac tggtataaaa gactaactgt cggattaaat
     1081 tctgaaaaac cctaggataa attgacttac gaagctaagt gagtaaaatg gcttcacttt
     1141 aattagctct aggtgtttat gggtattcct gttccacgca ctcatgtgtg tatacaatct
     1201 tctatagatg tgagacagat tgctgctccc ctctttcaat acaccaatat tcattatttt
     1261 tgttatggaa gaaattatcc aaacggcggc acatttacgc tccataccga tgtaaacttt
     1321 tatgattctt ggtttattca agaatttccg ttagtgctct cttttttaaa agaaggatta
     1381 cacctttggg atgattgcca aaatgcagat ctcattcgag tacgcttgaa tgactttaat
     1441 tatgggaacg gtttatatat agttcgtcat tttcccgaat attcagaaat tttcggcttt
     1501 gctcctgctc gtgaacaaaa catttccgat ctttatttaa ataatctaga tgtgttaaat
     1561 agatttgcga tgtattttaa ggataaagct tatcgtatta ttgagcgtgc ggaaaaagac
     1621 ctaatttttg ttcccaaaac gatgttaata gaaaaggaag aacttgcatc caataataat
     1681 ttagaacttt taagaaatac aacaaaaatt gaaatgtatg ctctttcccc tacgctcaat
     1741 ttagagctaa cgcaaagaga acatgaaagt tttaaaggat ttttgcaagg acaaactgca
     1801 gcacaaattg caagctcttt aaatttatct ccgaagtcgg tcgaaacctt tcttttaaaa
     1861 gtaaagaaaa agtttcaatg ctcgacccgc tcagatttgt taaaaagggc ctgggatttg
     1921 ggtattttac agtcgaatgg atggtttgat taattatttc aagcctaata catcttgcat
     1981 atcataaagc cctttggatt tattcgttat ccatcgggca gctaccagtg ctccttgagc
     2041 aaaagcctct ctagagcttg ttttatgcgc aatct
//
LOCUS       DASTIB010000155         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1034398, whole genome shotgun sequence.
ACCESSION   DASTIB010000155 DASTIB010000000
VERSION     DASTIB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034398"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /gene="thrS"
                     /locus_tag="VFP93_02655"
     CDS             <1..1326
                     /gene="thrS"
                     /locus_tag="VFP93_02655"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9843539.1"
                     /translation="AYWRGDSNNEMLQRIYGTAWRDQKSLSEYIVRLEESQKRDHRKL
                     AKRYDLFHIQDEAPGMVFWHPKGWTLYLIVQDYIRSLISQNGYQEIRTPEVVDSSLWE
                     KSGHLAMFSKEMFMVKTEDRSFAIKPMNCPCHVQVFNQELKSYRDLPYRLAEFGSCHR
                     NEPSGALHGLMRLRNFVQDDAHIFCTEDQIQDEVSNFIDLVFKTYQDFGFNDISIKLS
                     TRPEERVGSDEVWDKAEQALENALNQKNLTWSLNPGEGAFYGPKIEFSLHDCLNRVWQ
                     CGTMQVDFSMPVRLGAEYVTEASERKPPVMLHRAIFGSCERFIGILIEHYAGSFPAWL
                     APEQVAVLNITDKQASYAQEIAKNLQKSGIRVVTDLRNEKIGFKIREHTIQRVPFLAV
                     VGDQEVQTRSVSLRTREGEDLGVMALDEFEKKLLKACALPEIKLGGDNN"
     gene            1319..1879
                     /gene="infC"
                     /locus_tag="VFP93_02660"
     CDS             1319..1879
                     /gene="infC"
                     /locus_tag="VFP93_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9843540.1"
                     /translation="MISTTNSKKQRIRVNNAINSPKVRLVDPEGLLNNPLLGSGDLTG
                     IMNPRRALEMAEKLNLDLVEVSPNADPPVVRIMDYGKYLFGVKKAKKKQKAPKSKLKE
                     LKLRPVTGEGDYQVKLRNLKGFLEHGDKVKITIRFRGREIAHQDLGNRLLERIVKDIE
                     GFGQIEQTPKLEGRQMVMIIAPVSKK"
     gene            1893..2117
                     /gene="rpmI"
                     /locus_tag="VFP93_02665"
     CDS             1893..2117
                     /gene="rpmI"
                     /locus_tag="VFP93_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET9843541.1"
                     /translation="MSAKKKPARIKIKSKRGAQKRFKVTAKGKVRFRRAFRNHINTKR
                     SEKKERQSRRMGVLQPQDGKLVLRQMPYDV"
     gene            2138..2497
                     /gene="rplT"
                     /locus_tag="VFP93_02670"
     CDS             2138..2497
                     /gene="rplT"
                     /locus_tag="VFP93_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9843542.1"
                     /translation="MARVKRGVQARRRHKKVLKAAKGYYGAKSRTYKVARQAVFKAGQ
                     YAYRDRRQKKRQFRSLWIIRINAAARIHGLSYSRFMNGLKKAEVDIDRKVLSDIAIYD
                     MEAFAKLATTAKNALEA"
BASE COUNT          854 a          497 c          619 g          708 t
ORIGIN      
        1 gcttattggc gtggcgactc caacaacgaa atgttacaac gcatttatgg tacagcctgg
       61 cgcgatcaaa aatctctgtc tgaatatatt gtacgactgg aagaatcaca aaagcgtgat
      121 catcggaaat tggcaaagcg ctatgatttg tttcatattc aagacgaagc ccctggcatg
      181 gtcttttggc atcctaaagg ctggacttta tatttaattg tacaggacta tattcgctct
      241 ctaattagcc aaaatggcta tcaagagata agaacacctg aggtggtaga tagctctctc
      301 tgggagaaat caggccattt ggccatgttc agtaaagaaa tgtttatggt aaaaaccgaa
      361 gatcgtagtt ttgccataaa acctatgaac tgtccgtgtc atgtgcaagt ctttaatcaa
      421 gaattaaaaa gttatcgtga tttaccctac cgtttggccg aattcggttc ttgccatcgc
      481 aatgaaccgt ccggcgcttt gcatgggcta atgcgtttgc gcaattttgt tcaagacgat
      541 gcgcatatct tttgcacaga agatcaaata caagacgaag tatcgaattt catcgactta
      601 gtgtttaaaa cctaccaaga ttttggattt aacgacattt ccatcaaact ttccacgcgt
      661 cccgaagaac gggtgggtag tgatgaagtt tgggataaag ccgagcaagc gttagaaaac
      721 gcactaaacc aaaagaattt aacgtggagt ttaaacccag gtgagggtgc cttttatgga
      781 cccaaaattg agttctcttt acacgattgt ttaaatcgcg tttggcagtg tggcacgatg
      841 caagtggatt tttctatgcc agttcgctta ggtgctgaat atgtaaccga agctagtgaa
      901 agaaagccgc cggtcatgct gcatcgggcg atatttggtt cttgtgaacg ttttatcgga
      961 attctcattg agcattatgc gggatctttt cctgcatggt tagcccctga gcaagttgcc
     1021 gtccttaata ttaccgataa acaggcgtcc tatgcccaag aaattgcgaa aaatttacaa
     1081 aaaagcggca ttagggttgt gactgacttg agaaacgaga aaatagggtt taaaatccgg
     1141 gagcatacta ttcagcgtgt gccctttctt gcagtagtcg gtgatcaaga agtccaaacc
     1201 aggagtgtgt cattgagaac tcgagagggt gaagatttag gtgtaatggc gctagatgag
     1261 tttgaaaaga aacttttaaa agcttgtgcc ttaccagaga taaaattagg aggggataat
     1321 aattagtaca acaaatagta agaaacagcg tattcgtgtg aataatgcaa ttaattcacc
     1381 taaagtacga ctggtcgatc cagagggttt attaaataac ccattgcttg gatctgggga
     1441 tttgacgggc attatgaacc ctagacgcgc gcttgaaatg gcggagaaac ttaatcttga
     1501 tttagtagaa gtatctccca atgccgatcc ccccgtggtg cgtataatgg actatggtaa
     1561 atatcttttc ggcgttaaaa aagctaagaa gaagcaaaaa gcacccaagt caaaattaaa
     1621 agagttaaag ttacgtccgg ttacgggcga gggagactac caggtaaagc tacgcaacct
     1681 aaagggcttc ctagaacatg gagataaagt caagataacg attcgtttcc gagggcgcga
     1741 gattgcacac caagatttgg gcaatcggtt acttgaacgt attgttaaag acattgaagg
     1801 ctttggtcaa attgagcaaa cgccaaaatt agaaggtcga cagatggtga tgattattgc
     1861 gcctgtcagt aaaaaataaa tgcggagttt ttatgagtgc gaagaaaaaa ccagcacgta
     1921 ttaagataaa gtctaaacgg ggcgcacaaa agcgtttcaa agtgactgct aaaggaaaag
     1981 ttcgtttccg acgtgccttt agaaaccaca tcaacacaaa gcgttctgaa aagaaagaac
     2041 gtcaatccag aagaatgggt gttttgcagc cacaagacgg taaactcgtt ttgcgccaaa
     2101 tgccttatga tgtttaattg tgatgtggga ataaattatg gcaagagtta aacgtggcgt
     2161 ccaagcgcgt cgcagacata aaaaagtttt aaaagctgct aaaggttatt acggtgcaaa
     2221 gagccgtact tataaagtag cacgccaagc cgtcttcaaa gcgggtcaat acgcttatcg
     2281 tgatagaaga cagaaaaaac gtcaattcag aagcttatgg atcattcgta tcaatgctgc
     2341 tgctagaata cacggattat cctatagtcg atttatgaac ggcctgaaaa aagcagaagt
     2401 agacatcgat cgtaaagtct taagtgatat tgcgatttat gatatggaag cttttgcgaa
     2461 attagctaca accgcaaaaa acgcattaga agcttaaacg agtggtggga tttcccgcca
     2521 ctttttaaaa gggccccaca tcatgccaga attggtagac ttgcttgcac aagcgcaact
     2581 acgtattcaa gaagcccaat ctttaccaga acttgaaaaa gttcgcgtgg attttttagg
     2641 aaaaaaaggt tccattacgc accttttgaa agatttaa
//
LOCUS       DASTIB010000156         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_969484, whole genome shotgun sequence.
ACCESSION   DASTIB010000156 DASTIB010000000
VERSION     DASTIB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969484"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFP93_02675"
     CDS             complement(<1..866)
                     /locus_tag="VFP93_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843543.1"
                     /translation="MLNWPHILDVNRQDQNGMSPVHYAIIQKNFEELQKLTNVPCVFV
                     NLADKDNKMPLHYAIESNFIEAIALLLEKGADLHAKDKYGNSILHYVAAFGTTETIRL
                     ILKNKPNVVSNHMGLKPLDFAIHCNNFNFLYYANENLENPACRILPVNERRVKISAYL
                     NFIKLFWEGLAENSPKKHYLTLDDKITFFTLNKIIDHCLLSTRTFLSSSIPQFWQPLY
                     NKSKENELSLRKALNTMHRGVSEQLKVLQIAHLEYTSNYIQPIQVVINRFGNIPIPYN
                     ASTVHSWFFAFF"
     gene            complement(961..1980)
                     /locus_tag="VFP93_02680"
     CDS             complement(961..1980)
                     /locus_tag="VFP93_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843544.1"
                     /translation="MQGGVRNLTIANAQAAKITEELSSIEPALSPWHSFIHVLSNYYH
                     EQKLQENTDCELHLSLKENALSAFPKVSIAVVKNNEILFRLRVSEENQDFLLVAQNQN
                     EKHSIQINTSDIQAIDDFLTKNLQELKEIIAPDTNKVKEILEETTNKSQSAAKNLTKI
                     QTTFKGLVNNHITGLIVSVGISCLIAFYGKPIAAVFGRELFELFYRTVYGTPEYSWWN
                     PISIYNYNTYYFAPREHIGKLFYDHAHYILSTMGLGSWLVVRKVSQNLYAWIRGRKQS
                     PQPEASDVDILCSDLQNMTIKQPLSFQEQIAKQSALILSKRNKLPKEHEVNEENRKTL
                     PKMQC"
     gene            2266..2421
                     /locus_tag="VFP93_02685"
     CDS             2266..2421
                     /locus_tag="VFP93_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843545.1"
                     /translation="MTRALLDVFNRLREKFGLALLVRGNDAQAALPTGHRLARALHMY
                     LSVHVKD"
     gene            complement(2520..>3639)
                     /gene="pnp"
                     /locus_tag="VFP93_02690"
     CDS             complement(2520..>3639)
                     /gene="pnp"
                     /locus_tag="VFP93_02690"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9843546.1"
                     /translation="QTGVLPRTHGSAFFTRGETQAVVVATLGTGRDAQVIDNVIGEKK
                     ESFILHYNFPPYCVGEAGMVGSPKRREIGHGNLARRAIRRVLPDLKKFPYVMRVVSEI
                     TESNGSSSMASVCGTSLALMDAGVPIKSPVAGIAMGLIKEEDTFAILTDILGDEDHLG
                     DMDFKVAGTESGVTALQMDIKIDGITKEIMEVALQQAKSGRMHILRIMSQALSEARDN
                     LSEFAPRVHSIRVSPEKIKDVIGKGGATIKQLTEETGASIEITEAGDINIVASDQSIC
                     DDVVLRIQNLTAEVEVEKIYEGKIVKIVDFGAFVNILPGKDGLLHISQISDQRIENVA
                     DHLAVGQKVRVKVMGIDRQGRIKLSMRENAEQKMEQHS"
BASE COUNT         1069 a          710 c          652 g         1208 t
ORIGIN      
        1 tgaaaaaatg caaaaaacca agaatgtacc gtggaggcat tataaggaat aggtatatta
       61 ccaaagcgat taatcacaac ttgtatcggt tgtatataat tggatgtgta ctctagatga
      121 gcaatttgca gcactttcaa ctgctcactt acccctcggt gcatcgtatt taaggctttg
      181 cgcaaggaaa gttcattttc tttgctttta ttatacaatg gctgccagaa ctgtggtatt
      241 gaagagctta aaaacgttcg cgtgctcaac agacaatgat caataatttt atttaacgtg
      301 aagaaagtaa ttttatcgtc taatgtgaga taatgtttct ttgggctatt ttctgccaaa
      361 ccttcccaaa acaatttgat aaaatttaaa taggcactaa ttttcaccct tcgttcattt
      421 acaggcaata tccgacacgc agggttttct aaattttcat ttgcataata taaaaaatta
      481 aaattattac agtgaatagc aaaatcgagt ggctttaacc ccatgtgatt tgacacaaca
      541 ttgggcttat tttttaaaat taaacggata gtttccgtag ttccgaatgc cgcaacataa
      601 tgtagtatgg aatttccata tttatctttt gcatgaagat ccgcaccttt ttcaagtagc
      661 aaagcaatag cttcgatgaa gtttgactcg attgcatagt gcaacggcat tttattatct
      721 ttgtctgcca aattaacaaa tacacaagga acatttgtaa gtttttgcaa ctcttcaaaa
      781 tttttttgga ttatggcata gtgtactggt gacatgccat tttggtcttg acggttcaca
      841 tcgagaatat gaggccagtt tagcataatc ttgaagtccg cgagcaaaac taaactttag
      901 cacgagttaa attttagctc aactcaggag cggacctcaa tgtattttta tgctcacaat
      961 ttaacattgc attttaggaa gcgtttttct attttcctcg ttaacttcgt gttccttagg
     1021 tagtttatta cgttttgata aaatgagtgc agattgtttt gcaatttgtt cttgaaagct
     1081 caaaggttgc ttaatggtca tattttgcaa atcggagcat aaaatatcta catcgcttgc
     1141 ttctggttgt ggtgattgtt ttctccccct aatccacgca tataaatttt gagaaacttt
     1201 tctcaccaca agccatgaac caagacccat tgttgacaaa atataatgcg catgatcata
     1261 aaagagttta cctatatgtt cccgcggcgc gaaatagtaa gtattgtaat tgtaaatact
     1321 gataggattc caccaagaat attctggtgt gccgtataca gttcgataaa atagttcaaa
     1381 tagctctcga ccaaaaactg cagcaatggg tttgccgtaa aaagctatta aacaagaaat
     1441 acctaccgaa acaatgagcc ctgtaatatg attatttaca agacctttaa atgtggtttg
     1501 aatttttgta agatttttag ctgcagattg cgatttattc gtggtttctt ctagaatttc
     1561 ttttactttg tttgtatctg gcgcaataat ttccttaagt tcttgtaaat ttttcgttag
     1621 aaaatcatct atagcctgaa tgtcgcttgt atttatttgg atactatgtt tttcattttg
     1681 attttgtgcg actaagagaa aatcttggtt ttcttctgaa actcgtagcc tgaacaatat
     1741 ttcattattc tttacaactg ctatcgaaac tttaggaaag gcagataatg cattttcttt
     1801 aagacttaaa tgtaattcac aatccgtgtt ttcttgtagc ttttgctcat ggtaataatt
     1861 tgataaaaca tgaataaatg aatgccaggg tgacaacgca ggttcgatac tggataattc
     1921 ctctgttatt tttgccgctt gcgcatttgc gatcgttaaa ttccttaccc caccttgcat
     1981 aagctgcctc gctttctaag ttcgtatatt taagttataa gacaggctag ttatggattg
     2041 agatacaggc aatctgtgga cgaataacgg ttagcttttt cggatgattg gagagcacag
     2101 tgtaagtttg ttaacttatt tttagtattt tttgacaaaa ttattgttta tgaaattcct
     2161 tgattaataa aaaattttcc tgagttacgt tatggaagtt ttactgaaat atggaagtta
     2221 tcagagtaga aattattcaa gcgccctcaa gcgacaagaa gtcgcttgac acgagcatta
     2281 ctggatgtat tcaatcgttt acgtgaaaaa tttggactcg ccttactcgt tcgcgggaat
     2341 gacgcacaag cggccttacc gacaggacat cgcttagcac gagcattaca tatgtattta
     2401 agcgtccacg tgaaggattg aactctagta acttactttc agatttggaa gaagatcccg
     2461 cgtgcgcggg aatgacgtat tcgcgttcgc gggaaagaat tataaggaat gatggaaagt
     2521 taagagtgtt gttccatttt ttgctcagca ttttctctca tgctgagttt tattctgcct
     2581 tgtctatcaa tacccattac ttttacacgt actttttgac ccactgccaa atgatccgcc
     2641 acattttcaa tgcgttgatc actaatttgt gaaatatgca gtagcccatc tttaccaggt
     2701 aaaatattta caaacgcacc aaaatccaca attttaacta ttttcccttc gtaaattttc
     2761 tctacttcta cttccgctgt taaattctga atacgtagta ctacatcgtc acaaatagat
     2821 tgatcggaag ccacaatatt aatatcacct gcttcggtaa tctcaatact tgctccagtt
     2881 tcttccgtta attgtttaat ggtggcgcca cctttaccaa taacatcttt gattttttca
     2941 gggctcactc taatggaatg cacgcgtgga gcaaactcag acagattatc tcgcgcttcg
     3001 cttaacgctt gactcataat tcgtaaaata tgcatacgac cagacttggc ctgctgcagc
     3061 gcaacttcca taatctcttt tgttattccg tcgattttaa tatccatctg taaagcagta
     3121 acacccgatt cagtaccagc tactttaaag tccatgtcgc ctaaatgatc ttcatctcca
     3181 agaatatccg ttaaaattgc gaacgtgtct tcttctttaa taagacccat tgctattcca
     3241 gccacaggag acttaattgg cacccctgca tccattaagg ctaaagatgt accacaaacc
     3301 gatgccatgg aacttgaacc gttagattct gtaatctcag aaacaacgcg cattacataa
     3361 ggaaatttct tgagatctgg taaaacacgg cgaatggccc ttcttgctaa attaccatgg
     3421 ccaatttcac gacgtttcgg acttcccacc atgccagctt cacccacaca atagggaggg
     3481 aaattgtaat ggaggataaa agattctttt ttctcaccaa ttacattatc aatgacttgc
     3541 gcatcacgcc ctgttcctag agtggctaca actaccgctt gcgtttcgcc acgtgtaaag
     3601 aaggccgaac cgtgcgttct aggtaaaaca cctgtttgg
//
LOCUS       DASTIB010000157         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_186373, whole genome shotgun sequence.
ACCESSION   DASTIB010000157 DASTIB010000000
VERSION     DASTIB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186373"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2228)
                     /locus_tag="VFP93_02695"
     CDS             complement(<1..>2228)
                     /locus_tag="VFP93_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9843547.1"
                     /translation="ETLAAELAILYENIELYNVIQKHAADLQMESRKRKKVQEELRKN
                     EKMFRQFAENIDQLFFVASPTFDTIIYINSGYQKIFGRTSESLYQDPKSWINVVFEED
                     KKIVAEELDKIVYQEKNGSLKYRIVRPDGTIRYIYSKVSCIKDKKGRFINIIGIASDI
                     TEYMQNQKSVQLKNQIQDLFDKSLSLREVAPKLLQLLRVTFGWNIAELWLIDFSKNTI
                     RNIENSADSNNKYSEFLTIASHLAFPYAKDLPGEIWRLKAPIWIEDYLNHPNCPRNAI
                     AEKYKLRQALGFPLIYRKDIFGVITFFAHTMPKPDDYFMNMLSAIGNQIGEYINHKFT
                     TDQLLSLTKKDILTGLTNRVSMEEELDNILREKPNQNLAVCIVGIDRFQLVNQVFGFE
                     FGDVLLKNIAQRLIVYKPQGFLFRASGAKFGFVLLNVQSDNLHEFIEKLFLSFKSPFI
                     VAEQYLIENISIGISMYPKDGDNAKKLLQNAEIANRTAHLEGGNQVRFFEKQLTLASS
                     AKLILENDIKQALNHEEFCLYFQPKVDIQTQTITGFEALLRWKHPEKGMINPLEFIHL
                     AEETGLIIPLGDWVIDEACKTLKQLNALNKKLSIAINISIRQLMEHDFIKKLQQVIDT
                     YNIIPETLQLEVTESYILSQGPQFKEIVKFLKDTVGVSVAFDDFGTGYSALSYLTYAQ
                     IDVIKIDKSFINGIPNVANNVAIIRGIISIAQTLLIKTVAEGVETEQQWNFLLDAGCD
                     EV"
BASE COUNT          679 a          409 c          349 g          791 t
ORIGIN      
        1 gcacttcgtc gcaacctgca tctaagagaa agttccattg ttgttcagtt tcaactcctt
       61 ccgcaaccgt ttttatcaaa agcgtttgcg caatagaaat aatacctcga atgattgcaa
      121 cgttattagc aacgttggga atgccattga taaatgattt atcaattttt atgacatcaa
      181 tttgagcata tgttaagtag cttaacgcgg aatatcctgt accaaaatca tcgaaagcta
      241 cacttacacc aaccgtatct ttcagaaatt taacaatttc tttaaattga ggaccttgac
      301 ttaaaatata actttcggtt acttctaact gtaaagtttc cggtataata ttataagtat
      361 caatcacttg ctgcaatttc tttataaaat catgctccat gagttgccgg atggatatat
      421 taattgcgat agacaatttt ttatttagtg cattcaattg cttgagcgtt ttgcacgctt
      481 catcaatcac ccaatcgcct aacggaataa tcaaacctgt ttcctctgct aaatgtataa
      541 attcaagagg attaatcata cctttttccg gatgtttcca tcgcaacaga gcctcaaacc
      601 ctgttatcgt ttgggtttgg atatccactt ttggttgaaa gtataaacaa aattcttcgt
      661 gattgagggc ttgtttaata tcattttcta aaattaattt agcagaagag gccaatgtta
      721 attgcttctc aaaaaagcga acttgatttc ctccttctaa gtgtgcagtt cgattggcta
      781 tttccgcatt ttgcaacaat tttttggcat tatccccgtc tttaggatac atgctaatac
      841 ctatgcttat attttcaatt aaatattgct ccgcgacgat aaaaggactt ttaaaggata
      901 gaaacaattt ttctataaat tcatggagat tatcgctttg aacattcagc aaaacaaatc
      961 caaattttgc accagatgct ctgaatagaa aaccttgtgg tttatatacg ataagtcttt
     1021 gtgcaatgtt ttttaaaaga acatcaccga attcaaaacc gaatacttga ttgactaatt
     1081 gaaatcgatc aatccccaca atacatactg ctaggttttg atttggcttt tctcttaaaa
     1141 tattatcaag ttcttcttcc atggaaactc gattggtaag tccggttagt atatcttttt
     1201 ttgtaagact aagcagttga tcggttgtga atttatgatt aatgtattca cctatttgat
     1261 tccctatggc cgaaagcatg ttcataaaat aatcatcggg ttttggcatc gtatgcgcaa
     1321 aaaaggtaat caccccaaaa atatcttttc tgtatattaa ggggaatcct aaagcttgcc
     1381 gaagcttata tttttctgca atagcatttc tgggacaatt aggatgattt aaataatctt
     1441 ctatccagat gggagctttt aatcgccaaa tttcgccggg tagatcttta gcataaggaa
     1501 aagctaaatg actggcaatc gttaaaaact cactatattt attattggaa tcagctgaat
     1561 tttctatatt tcgtatcgta ttttttgaaa agtcaatgag ccataattca gctatattcc
     1621 acccaaatgt tacacgcaaa agttgcaata attttggagc aacctcgcgt aaggataagc
     1681 ttttatcaaa taaatcttga atttgatttt ttagctgtac acttttttgg ttttgcatat
     1741 attccgtaat atcagatgca attcctataa tatttataaa acgacccttc ttgtccttaa
     1801 tacaagacac tttggaataa atataacgga tcgttccatc gggtcgaaca attcgatatt
     1861 ttaaagagcc atttttttct tgatacacga ttttatcgag ttcttctgcg acaattttct
     1921 tatcctcttc aaaaacaaca tttatccagc tttttggatc ttgatataag ctttcactcg
     1981 ttctcccaaa aattttttga tatcccgaat taatgtaaat tatggtgtca aaggtgggac
     2041 tagcaacgaa aaataactgg tcgatattct ccgcaaattg tctaaacatt ttttcatttt
     2101 tacgcaattc ttcttgaact tttttgcgct tgcggctttc catttgtaaa tctgcggcgt
     2161 gcttttgaat aacattgtaa agttctatat tttcgtaaag tattgccagc tcagctgcca
     2221 aagtttca
//
LOCUS       DASTIB010000158         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1341638, whole genome shotgun sequence.
ACCESSION   DASTIB010000158 DASTIB010000000
VERSION     DASTIB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341638"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="folP"
                     /locus_tag="VFP93_02700"
     CDS             <1..742
                     /gene="folP"
                     /locus_tag="VFP93_02700"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET9843548.1"
                     /translation="DGGAYRDLSKALFRCEQMIEEGANIIDIGGESTRPKASPVSSAV
                     ELERVLPVIEAIRSRMDIVLSVDTRHFEVMEACLKLGVEWINDISALQEPNALHCVAH
                     FKPYIVLMHMQGLPSTMQDNPTYCEIIQDVKSFFTQRIQVCIEAGIPSEKIVLDPGFG
                     FGKNLQHNLTLLNRLDELNQTFPLLIGLSRKSMFYHLLGRAKEDRLPASLAATVIAVL
                     KGANIIRTHDVKATADCVAVVSALKKLR"
     gene            751..2127
                     /gene="glmM"
                     /locus_tag="VFP93_02705"
     CDS             751..2127
                     /gene="glmM"
                     /locus_tag="VFP93_02705"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005903956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9843549.1"
                     /translation="MNMEKKYFGTDGIRGKVGVHPITPQFIMHLGWAFGRTLQDREYK
                     KVLIGKDTRLSGYMLESALEAGLVAAGVDVVQLGPMPTPGISFLTKTLPVAGGIVISA
                     SHNPYHDNGIKFFNEKGFKLGDPIELAIEAELQRDLMTVENEKVGRAERLAKAPNLYA
                     DYCYQLFYNKVKLSGLHCVIDCAHGAMYHIAPKLFERFGAKVTVIGCKPNGLNINESS
                     GSLYPENLVQKVQATQANIGIAFDGDGDRVLLIDEKGEILDGDEILYIIARAKLATGT
                     LQGGVVGTLMTNFGVERAFKDLKIPFVRSAVGDRYVLDELLTKNWQLGGETSGHLIDL
                     SKTTTGDGMVAALLVIEEMVRTQKSLFELKQGLNKYTQTMINVPVPQGKFIVASNQVQ
                     QILKEQERRLQGFGRIVLRPSGTEPLVRVMVEAEHTDMTQIVAQTIADVVRACADRMK
                     HKDSASDL"
     gene            complement(2122..>2544)
                     /locus_tag="VFP93_02710"
     CDS             complement(2122..>2544)
                     /locus_tag="VFP93_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843550.1"
                     /translation="QTFFPKRASNARSTKLLYPSIKELNKLEAEMLKQLEYRLHLRGS
                     LEHFIQLLNKLIATKNMFYELGWKELQKYARSSQKKMLTFKQYEAWCQITYKSLKELT
                     SDPHEENVESPSSTRWSKHHTFKHDVSDITHKIKKVQL"
BASE COUNT          786 a          438 c          568 g          752 t
ORIGIN      
        1 tgacgggggt gcttataggg atctttcgaa agcgcttttt cgatgcgaac aaatgatcga
       61 agagggcgcg aatataatag atattggcgg agagtccacg cgaccgaaag caagtcctgt
      121 ttcttctgcc gtagaactag aacgtgtgct gcctgtaata gaagcgattc gttcgcgtat
      181 ggatatagtg ttatcggttg atacgcggca ttttgaagta atggaagcat gcttaaaatt
      241 aggtgtagag tggattaacg atatatcggc tttgcaagag ccaaatgcgc tacattgtgt
      301 tgcgcatttt aagccttata ttgtgcttat gcatatgcaa ggtctaccct ccactatgca
      361 agataatcca acgtattgcg aaatcataca agatgtaaaa agctttttta cccagagaat
      421 tcaagtgtgt attgaagcgg gaattccatc agagaaaatc gttttagatc ccggcttcgg
      481 ttttggtaaa aatttacagc ataatttaac cttgctaaat cgattagacg aattgaatca
      541 aacatttccc ttgttaattg gcttgtcgcg taaaagtatg ttttatcatc tattaggacg
      601 cgcgaaagaa gatagattgc ctgcgagctt agctgcaaca gtcattgcag tcttaaaagg
      661 cgcaaacatt ataagaacac atgatgtaaa agctaccgca gattgtgtcg cagtggtaag
      721 cgccttaaaa aaattacgtt gaggactttt atgaacatgg aaaaaaaata ttttggaacc
      781 gatggcatcc gaggaaaagt cggtgtacat cctattacac cccaatttat tatgcattta
      841 ggctgggctt ttggcagaac acttcaagat cgagaatata aaaaagtgct cataggaaaa
      901 gacacccgat tatcgggtta tatgctggaa tccgcattgg aagcaggttt ggttgctgct
      961 ggagtcgatg tagtgcagct aggacctatg ccaactccgg gtataagttt tttaacaaag
     1021 actttaccgg tagcgggtgg tattgtcata agtgcttcgc ataatccata ccacgacaat
     1081 gggattaaat tttttaatga aaaaggattt aaattaggcg atcccatcga attagcaatt
     1141 gaagcggaat tacagcgcga tttaatgacc gtagaaaatg agaaagtggg tcgtgctgag
     1201 cgtttggcta aagcccccaa tttgtacgcc gattattgct atcaactttt ttataataaa
     1261 gtaaagttga gcggtttgca ttgtgtaatc gattgtgctc atggcgcaat gtatcatata
     1321 gctcctaaat tatttgagag atttggggct aaagtaaccg tcataggttg caaacccaat
     1381 ggattaaata taaatgaaag ttcgggttct ttataccctg aaaatctagt gcaaaaagtg
     1441 caagcaacgc aagcaaacat aggaattgcc tttgatggag atggcgatcg tgttctttta
     1501 atcgatgaaa aaggagaaat tctcgacgga gatgagattt tatatattat tgcacgcgct
     1561 aaattagcta caggcacctt acaagggggc gttgttggca cgctgatgac aaattttggt
     1621 gtagaacgcg cttttaaaga tttaaaaatt ccgtttgtac gctctgcggt aggtgatcgc
     1681 tatgttctcg atgaattgct tactaaaaat tggcagttag gtggagaaac ctcaggtcat
     1741 ctcatcgact tatctaaaac cacaacaggc gatggcatgg tcgctgcatt gttagttata
     1801 gaggaaatgg tgcgcacgca gaaatcctta tttgaattga aacaagggct aaataaatat
     1861 acgcaaacca tgatcaatgt tcctgtccca caaggaaaat ttattgtggc gtcaaatcag
     1921 gtgcaacaaa ttttaaaaga acaagagcga aggttacaag gctttggaag aattgtatta
     1981 cgcccttcag gcacggaacc gctcgtacgt gtgatggtag aagcagaaca tacagatatg
     2041 acacaaatag tggcacaaac tatcgcagat gtggttcgtg catgcgccga tcgcatgaag
     2101 cacaaagata gtgcttcgga tctataattg tacctttttg attttgtgcg ttatatctga
     2161 gacatcgtgc ttaaaagtat gatgtttgct ccatcttgta gaagaagggc tttcaacatt
     2221 ctcttcatga ggatctgatg ttaattcttt aagcgactta taagtaattt gacaccatgc
     2281 ctcatactgc ttgaaagtga gcattttttt ttgtgaagag cgtgcatatt tttgtaattc
     2341 tttccatccc aattcataaa acatattctt tgtagcaatt aacttattta aaagttgaat
     2401 aaaatgttca agagaaccac gaagatgtaa acggtattcc aattgcttta acatttcagc
     2461 ttctaattta tttaactctt taatagaagg gtacagtaat tttgtagatc gggcgtttga
     2521 agccctttta ggaaaaaacg tctg
//
LOCUS       DASTIB010000159         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_760017, whole genome shotgun sequence.
ACCESSION   DASTIB010000159 DASTIB010000000
VERSION     DASTIB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760017"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFP93_02715"
     CDS             <1..900
                     /locus_tag="VFP93_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843551.1"
                     /translation="IGAATTQIPGNTGTVLDADTFTSVFNQLQLFADSTTTQFDTNLD
                     GTPGLNFGDRFSDNGTAFITDFLPPLGDDEGISFLSEITVAWEGLKGITTSDLHPINA
                     FGDVSQTISYDPNNTVLNFYFHGDASGPNANFGSSVGANDNTGFTDGQKILEIQVTGG
                     DGTNTFDQNGNFLSGSSILMGEVVFALEDFWWFDDGDTIPGTGLENDFNTLIGLPVPI
                     LLTANIDQNTDEVITDFSRTGAPGPDGFGDILFDVHSTHDGSLDFAMAQVPTPQTFIL
                     LGLGLVLFPLFGRRILTTSLSKN"
     gene            916..1152
                     /locus_tag="VFP93_02720"
     CDS             916..1152
                     /locus_tag="VFP93_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843552.1"
                     /translation="MWHLKTKLGTFWIIESDKEAQKYYLGCEDDKLGEYNSLDKILDD
                     VCNQETGFLKWDEQLKVTAPHDIGEWEEGPPENW"
     gene            complement(1153..1305)
                     /locus_tag="VFP93_02725"
     CDS             complement(1153..1305)
                     /locus_tag="VFP93_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843553.1"
                     /translation="MMKQKKSGKAFDYKVASTFSHGPIHSSYMRKNQSPILRPVAIKK
                     SPLQQR"
     gene            1565..1786
                     /gene="rpmE"
                     /locus_tag="VFP93_02730"
     CDS             1565..1786
                     /gene="rpmE"
                     /locus_tag="VFP93_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET9843554.1"
                     /translation="MKPEIHPNYKEVTVLCACGNEFITRSTYGREQIKLDVCNDCHPF
                     YTGKQKLVDTAGRIDKFYGRYKKKETDTK"
     gene            1884..>2263
                     /locus_tag="VFP93_02735"
     CDS             1884..>2263
                     /locus_tag="VFP93_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9843555.1"
                     /translation="MAQKDDLKQKALEYHAKPRPGKLSIQITKPTNTQKDLALAYTPG
                     VAEPVRAIKQDPSLAALYTAKSNLVGVITNGTAVLGLGNVGPLASKPVMEGKAVLFKR
                     FADVDVFDIEIEASNPQNFIETVSR"
BASE COUNT          699 a          415 c          479 g          670 t
ORIGIN      
        1 atcggggctg cgaccacaca aatccctgga aatacaggca cggttttgga tgctgatacc
       61 ttcacatccg tttttaacca attacaatta tttgcggact ctacaacgac gcaattcgat
      121 accaatcttg atggaacacc agggcttaat tttggcgata ggttttccga taatggcaca
      181 gcctttatca ccgacttttt gcccccttta ggcgatgatg aaggaatcag ctttttatcg
      241 gaaattaccg tagcttggga aggactaaaa ggcataacta cttcggattt gcatcccata
      301 aatgcttttg gcgatgtttc gcaaaccatt tcttatgatc caaataatac cgttttgaat
      361 ttttattttc atggagatgc ctcaggacca aatgcaaatt ttggctcgag tgtgggggcc
      421 aacgacaaca cgggtttcac ggacgggcaa aaaattttag aaatacaggt taccggagga
      481 gatggtacta atacttttga tcaaaatgga aacttcttaa gtggatcatc tattttgatg
      541 ggtgaagtcg tttttgcact cgaagatttt tggtggttcg atgatgggga tacgattcca
      601 ggtacgggtt tagaaaatga ttttaatact ctcataggac tgccagttcc catacttctc
      661 actgccaata ttgatcaaaa taccgacgag gttataaccg atttttctcg cacaggagct
      721 ccaggtccgg atggttttgg tgatatttta tttgatgtcc attccaccca tgacggcagt
      781 ttagattttg ctatggcgca agtccccacg ccacaaactt ttatattgtt gggtttaggg
      841 ttagtgctat tccccctatt tggacggaga attttaacta catctttgtc taagaattaa
      901 aaattaggag ggcgtatgtg gcatttaaaa accaaattag gtactttttg gatcatcgaa
      961 agtgacaaag aggcacaaaa gtactactta ggttgtgaag acgacaaatt aggggagtat
     1021 aattctctcg ataaaatttt ggatgatgtc tgtaaccaag aaacgggatt tttaaaatgg
     1081 gatgaacaat tgaaagtaac tgcgccccat gacattggcg aatgggaaga aggccctcca
     1141 gaaaattggt agttagcgtt gttgaagcgg tgattttttt atagcaacag gtcttaaaat
     1201 aggggattga tttttacgca tataacttga atgaatcgga ccatgggaaa atgtggatgc
     1261 cactttataa tcaaatgctt ttccagattt tttttgtttc atcatttccg ctcctctctc
     1321 ggtagccatt aaaaaatttc tacgcaatag attcaagctg agtaagcttg gcggtgtttt
     1381 atggaagatt aacggtggat agggatctaa tttcaaaaaa agtttgaatt attactaata
     1441 tttaaaaaag gacagttatc ttaatcacct tgcggtccag aactaggttc gatattatgc
     1501 aaaacttaga atttaacact atatttactc gtttcgtgta tacacactga gagcagagga
     1561 tcctatgaag ccagaaattc atccaaatta caaagaagtt accgttttat gcgcctgcgg
     1621 taatgaattt attactcgct ctacttatgg gcgcgaacag ataaagctag atgtatgtaa
     1681 tgattgtcat cctttttaca caggaaaaca aaagctagtc gatactgcag gtagaatcga
     1741 taagttctat ggcaggtata aaaagaaaga aaccgacacc aaataatttt ttttattcaa
     1801 agcaataggc gcattggcgc cttttttgtc gtatccaaca taggaagggg aacatggaat
     1861 ggttgtaacc acttgagggc atcatggctc agaaagacga tttaaaacaa aaagcacttg
     1921 aatatcatgc taaaccaaga cctggaaagt taagtattca aatcactaaa ccaaccaata
     1981 cccaaaaaga ccttgcctta gcttatacac ccggagtggc agagccagtc cgagctatca
     2041 aacaagaccc ttctctggcc gcactttata cggctaaatc aaatttagta ggtgttataa
     2101 caaacggcac agccgtatta ggacttggga atgtgggtcc cttagcttct aaaccggtaa
     2161 tggaaggtaa agcagtatta tttaagcgtt ttgccgatgt ggatgtgttt gatatcgaaa
     2221 tagaggcatc aaatccacaa aatttcattg aaacggtatc tcg
//
LOCUS       DASTIB010000160         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_916705, whole genome shotgun sequence.
ACCESSION   DASTIB010000160 DASTIB010000000
VERSION     DASTIB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916705"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..455
                     /locus_tag="VFP93_02740"
     CDS             39..455
                     /locus_tag="VFP93_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HET9843556.1"
                     /translation="MKDSVGFTIIELMFVLAIIGILAAIAIPAYNFYTVRTRVAELIN
                     IASTAKTAVSEYRLSNGTMPVSNAQAEVTDVITEYVAGLSIGAAGVITVTANHENLGT
                     GSPFAIVLTPTFTNGAVTWSCSASGATQFAPASCRK"
     gene            470..1348
                     /locus_tag="VFP93_02745"
     CDS             470..1348
                     /locus_tag="VFP93_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9843557.1"
                     /translation="MLQQNKCIKLSIIIPACNEKEGLSRILPQLVDNFPHAEILIIDD
                     GSTDDTAKYARSLGVRVISHPHRLGNGAAIKTGARAAQGEVFVLMDADGQHSIGDIQR
                     LLETFQEGYDMVVGARSRAGQANFVRYLGNRFYNWIATLIVNQKVQDLTSGFRVVRAK
                     KFKEFLHLLPNGFSYPSTITLAFFRMGYPIRYIPIDVFKRLGKSHLRPLSDGLRFFIL
                     IYKITILYSPLKIFLPLGFIHLALGLLNYAYTFATSGRFTNMSAVFLTSAIIIFLIGL
                     VSEQITILIYRGLRDS"
     gene            1345..2388
                     /locus_tag="VFP93_02750"
     CDS             1345..2388
                     /locus_tag="VFP93_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HET9843558.1"
                     /translation="MKILCDVCHPAHVHFFKNAISQFKTLGHEVLITARNKDCTLNLL
                     NFFKLDHTVLTSSRLTLPLVRLGCELVLRNKALLTVVREWKPDIMIGVGGIFIAHIRT
                     ITRIPAIIFYDGDNALLQNILTYPFASKIYVPDAYQGWTLSYKTIRYAGFHELAYLHP
                     KYFLPSKICAIQNGLHPEKPTIMIRLVAWKASHDFGHKGLDREHLLKWIENNSAHYHF
                     LVCAESNVPERLKPYVFRSHPAHLHHVLAYCHGFLGESPTMAMEAAIMGVPALYVSTI
                     QCGNVTRLQGDFQLIQCFNPPVFSFDYALPFFERYAKCFWMQRRADLLHATVDVTQLI
                     LNSIFDSEYAPLL"
     gene            2400..3341
                     /locus_tag="VFP93_02755"
     CDS             2400..3341
                     /locus_tag="VFP93_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9843559.1"
                     /translation="MNIVVIFLVFLGAAYLIVKNEQDFSLLFSQLNWWVFFLILPLQI
                     PLIGLGGYPFKVLIAKFGYILPWREWMGLSFIATLLNQILPYRPGIAFRYFYLKKKFH
                     LPLSLFSLATMLYMLMVTFTSALWVFIPSFFVGLPKKMEHEVATGLIYVLFLIIILIF
                     THILLRFLTQKNLLHRYLKRFTQSYRTLFASPQILFLSWFIICFIQLLSVLTFYLTFM
                     SLNYPILLGHCFFLVGITTLSLLFPLTPGNIGILETAIGGLTHALYNNFGLGFSAMLL
                     YRLAQLSVSLIGGSLFSFILLKHFLPAQKDFSKMGKL"
     gene            3413..>4123
                     /locus_tag="VFP93_02760"
     CDS             3413..>4123
                     /locus_tag="VFP93_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9843560.1"
                     /translation="MASQQNINISGLARKLVQAEVLDEQSATIAVEQSTRLKSPFVQH
                     LVENNLAHPQKIAVIASAEFGIPLLDLNVIDLDVLPKELVSEKLIRQHHALPLFKRGN
                     RLYVGMSDPTNLQALDEFKFHTGISTEAIIVEEDKLAEGIERILSARDASALSNLEDT
                     NLDDLDITSIEDDETRDEMGDSDVDDAPIVRFVNKVLLDAINKGASDIHFEPYEKMYR
                     VRFRQDGILYEFANPPVNL"
BASE COUNT         1158 a          800 c          797 g         1368 t
ORIGIN      
        1 ttaagaggtg atataaccac ttaaagggta tctactgcat gaaagattct gtcggtttca
       61 caataataga attaatgttt gtgcttgcta tcattggtat tttagccgct attgctatac
      121 ctgcttacaa tttttatacc gtaagaaccc gtgttgcaga gttaatcaat attgcctcga
      181 ctgcaaaaac ggctgtgtct gaatatcggc tgtctaatgg aaccatgcca gtttccaatg
      241 ctcaagctga agtaaccgat gtaattacgg agtatgtagc aggccttagt ataggtgccg
      301 caggtgtaat aacggtgaca gcgaatcacg aaaatttagg gacaggaagc ccctttgcaa
      361 ttgtactaac acccactttt acgaatggtg cggtcacgtg gagttgcagc gctagtggtg
      421 cgacgcaatt tgctcccgct tcctgccgta aataaaggta aaagttaaga tgttgcagca
      481 aaataaatgt ataaagctaa gcattatcat cccagcttgt aatgaaaaag aaggactgtc
      541 tcgcatactt cctcagttag ttgataactt ccctcatgca gaaatattaa ttatcgatga
      601 tggatccaca gatgacacgg ctaaatatgc acgctctttg ggagttcgcg ttatttctca
      661 tccccatcgt ttaggaaatg gagccgccat aaaaacaggc gctcgcgcag cccaaggaga
      721 agtctttgta cttatggatg cagatggtca gcattctata ggtgacatcc aaagattatt
      781 agaaactttt caagaaggat acgacatggt ggtaggcgct cgatccagag cgggacaagc
      841 aaattttgtg cgttatttag gtaatcgttt ctataactgg atcgccacat taattgtaaa
      901 tcaaaaagta caggatctga cctccggctt tcgtgtcgta cgggcaaaaa aatttaaaga
      961 atttttacat ttgttgccga atggtttttc ttacccctct acgataacac tcgctttttt
     1021 taggatggga taccctatac gctatatccc tattgatgtt tttaaacgcc tgggaaaaag
     1081 tcatttacgc cctttatcgg atgggttaag attttttatc ctcatttata aaattaccat
     1141 tctctattct cccctaaaaa tttttctgcc attaggtttt attcatttag ccttaggttt
     1201 gttaaactat gcatatacct ttgcaaccag cgggcgcttt accaacatga gcgctgtttt
     1261 cctaaccagc gcaatcatta tatttctcat tggacttgtt tccgaacaaa tcactatttt
     1321 aatttaccga ggcctgcgcg attcatgaaa attttatgtg atgtgtgtca tcccgcacat
     1381 gttcactttt ttaagaatgc catttcgcaa tttaaaaccc tggggcatga agttcttatt
     1441 acggcaagaa ataaagattg tacgctcaat ttattaaatt tttttaaact ggaccacacg
     1501 gtattaacta gttcacgttt gacgcttccc cttgtacgcc tcggatgcga acttgtctta
     1561 cgaaataagg ctttacttac ggtagtgagg gaatggaaac ccgatattat gataggagtc
     1621 ggtggcatat ttatcgcgca tattcgtact atcacgcgca tcccagcgat tatcttttat
     1681 gatggagaca atgccctact acaaaatata ttaacgtatc cttttgcatc caaaatttat
     1741 gtgccggatg cgtatcaagg atggacatta tcttataaaa caattcgtta tgctggtttt
     1801 catgagcttg cctatttgca cccgaagtat tttttacctt ccaagatctg tgctatccaa
     1861 aatgggttgc atcccgaaaa gcctactatc atgattcgtt tagttgcgtg gaaagccagt
     1921 catgattttg gtcataaagg tttggatcgt gaacatttac taaaatggat agagaacaat
     1981 tctgcccatt atcatttttt ggtttgtgcc gagagtaatg taccagaaag attaaaaccg
     2041 tatgtgttcc ggtcgcatcc tgcccattta catcatgtat tagcctattg tcatgggttt
     2101 ttgggagaaa gtcctactat ggccatggaa gccgcaatta tgggagtgcc tgcattgtat
     2161 gtttctacca ttcaatgtgg taacgtgaca cgtcttcaag gcgactttca gttaattcaa
     2221 tgtttcaacc cccctgtatt ttcctttgac tatgccttac ccttttttga aagatatgca
     2281 aagtgctttt ggatgcaaag acgcgcagat ttactccatg cgactgtaga tgtaacgcaa
     2341 ttaatcttga atagtatttt tgacagtgaa tatgcgccgt tactatgaaa agaaagcttt
     2401 tgaatatagt tgttatcttt ctagtttttt taggcgctgc gtatttaata gtcaaaaatg
     2461 aacaagattt ctccttactg ttttctcaat taaattggtg ggtgtttttt ttaattttgc
     2521 cattacaaat accgcttata ggccttggag gttacccctt taaagtatta attgcgaagt
     2581 ttggctatat tcttccgtgg cgtgaatgga tgggattaag ctttattgcc actttattaa
     2641 atcaaatttt accttatcgt ccaggcattg cttttcgtta tttttattta aagaaaaaat
     2701 tccatttgcc tttatctttg ttcagcttag caactatgct ttatatgctc atggtaacct
     2761 tcacctccgc gctttgggta tttataccct cgttttttgt aggcttaccg aagaaaatgg
     2821 agcacgaagt tgccacagga ttgatttacg tattattttt aatcatcatt ttgattttta
     2881 cgcatatttt gttgcgtttt ttaacacaaa aaaatttgtt acaccgctat cttaagcgat
     2941 tcacgcaatc ttaccgtaca cttttcgcat ctccgcaaat acttttttta agttggttta
     3001 ttatatgctt catacagtta ctatcggtat taacttttta tttaaccttt atgtcgttaa
     3061 attaccccat cttactgggt cactgctttt tcttagtggg gataaccacg ctaagcttgc
     3121 tttttcccct tactcctgga aatattggca tccttgaaac ggcaattggt ggtttaacac
     3181 atgctttata caataatttt gggctcggtt ttagcgcgat gctgttatac cgtttagcgc
     3241 aactgagtgt ttctttgatc gggggaagtt tatttagctt tattctgctt aaacactttt
     3301 tacctgcgca aaaagacttt tctaaaatgg gcaaattata agtagctcag ctaaacttag
     3361 gatacattcc ttagttaaaa tccgttgtaa taagcagacg tcaaaataaa acatggcatc
     3421 acaacaaaac attaacatta gtggattagc acgcaaactt gtccaggctg aggttttgga
     3481 tgaacaatct gcaaccattg ctgttgaaca atccacaagg cttaaatccc cttttgtaca
     3541 gcatctagtg gaaaataatc ttgcacatcc gcagaaaatt gcagtgatcg cttctgccga
     3601 atttggaatt cctttattag atttaaatgt catagattta gatgttttgc caaaagaatt
     3661 agtcagtgaa aagcttattc gccaacatca tgccttaccc ctttttaaaa gaggtaatcg
     3721 attgtatgtg ggcatgtcgg atccaaccaa cttacaagca ttagatgaat ttaagtttca
     3781 tacgggaatt agcactgaag ctattattgt cgaggaagat aaattagccg aaggcattga
     3841 acgtattcta tccgcacgcg atgcaagtgc gctgagcaac cttgaagata ctaatttaga
     3901 tgatctggat attactagta tcgaagatga tgaaacacgc gatgaaatgg gcgatagcga
     3961 tgtggacgac gcgcccattg tgcgatttgt gaacaaagtt ttattagatg ccatcaacaa
     4021 aggagcttcc gatattcact ttgagcccta tgaaaaaatg tatcgcgtcc ggtttcgtca
     4081 agatggtatt ttatacgaat ttgcaaaccc gcctgttaat ttg
//
LOCUS       DASTIB010000161         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_749708, whole genome shotgun sequence.
ACCESSION   DASTIB010000161 DASTIB010000000
VERSION     DASTIB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749708"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2963)
                     /locus_tag="VFP93_02765"
     CDS             complement(<1..2963)
                     /locus_tag="VFP93_02765"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HET9843561.1"
                     /translation="MKGKSSATTASASAGIGGVVGGLLFGVSGGVGHSGSNASQDSLR
                     QTSASDLQKVRDRLVQSANAVRSQRSTVIQTVAQGERFEVSSETIANYNHCHAITIQY
                     FEVLRHFKVKQRFAGARECLLVPLLMGQFDLDKTLRWKEALQNTLLDNTLVKGFEAAQ
                     RVKNEWTDSNFPSGTYASEKILHASGNFQIQFIIRRPDDKLVETDDHAKPIYGPAGMI
                     VGYQKKNIEDLNEDNWQSLIPFMGADTPRGFYDMHLKNARNKDEVFHRLLGEKIALSF
                     VDKLTFHVADEAGNEIASMNFDSSLTSRYRKEGTLNISVRFMGPSTFSRDKIHYIKIR
                     CGAANALPAFSSINVQSGFIRYKTPHYEGFLCKFQAINDDLSPSDGVVLYAGPSGDEL
                     KDPRKEDLHLVNKLIDHLNDNLEYFHTAIWMQLSEQRRFMLLDGIILPGEKGLGRSLA
                     SLVENELIGIVGNSLVFPVAQGLNLDPNFGLADSLTDYYMVSAADPVNISIPTKGVYA
                     EAMMGKCNSCEEKDESRFWRWEESPIPDSPTAINPINTDSRRAEPGNLQPAALPAPML
                     NIQNAPNVPDPTGLAGTLSLLGKGDAFRDITGLAQNQTNALEALKASFEATKMFGQEA
                     AKLEMQKMMEKRLDNALKSINNNPNLDKKQKADLTEKALNAYLGAGAQTNQPAKNEND
                     DKLQDGINKNLDKVNKSKSGEVAISKPDGTKVNTKFNSGEEEGANIETRLGKGGITPI
                     KQDNANACWASVATMMMRWKDSVQYTIEQVLTKAGQNFLDLYTQQQPLPVNVKDTFIS
                     SLGMTGEPLSHYTGDKYREWLIAFGPLWVTTDSDETKGFSAHARIITGISNDLQTLEL
                     IDPAQGKKVIQSFEDFIKSFKEVITDSPLELQTQVVHFTDTLASSGEGADPSPDDIAK
                     SLNEYDPADVNAIIINQAEFTTGLTGITNYKDWKTNNAVPHNKSKNPYRDPHGIIHLV
                     LHESAADTGDGFDDS"
BASE COUNT          807 a          634 c          592 g          977 t
ORIGIN      
        1 tccgaatcgt caaaaccatc gcctgtgtct gccgcactct catgtaatac taagtggatg
       61 attccatggg gatcgcgata gggatttttg cttttattat gtggtacagc gttattggtt
      121 ttccaatctt tataattggt aatccccgtt aagcctgtcg taaattcggc ttgattgata
      181 attatagcgt tgacatcggc tggatcgtat tcatttaaac tttttgcgat atcatcggga
      241 gacgggtcgg caccttctcc agacgatgcc aaagtatccg taaaatgcac aacttgcgtt
      301 tgcaattcca aggggctatc ggtaataact tctttaaacg attttataaa atcttcaaat
      361 gattgaataa cttttttacc ttgcgcaggg tcaattaatt ccaaggtttg taggtcattg
      421 cttattccgg taataatacg agcatgggcg gagaatcctt ttgtttcgtc cgaatcggtg
      481 gtgacccaaa gtggtccaaa agctataagc cattcgcgat atttatctcc ggtataatgc
      541 gaaagcggtt ctccggtcat acctaaactg ctaataaagg tatcttttac attgaccggt
      601 agtggttgtt gttgcgtata gagatctaaa aagttttggc ctgctttggt tagcacttgt
      661 tcaatggtat attgcacgct atctttccaa cgcatcatca tcgttgcaac actcgcccaa
      721 caagcattag cattgtcttg tttgattgga gtaatcccgc ccttccctaa ccgcgtttca
      781 atgttagcgc cttcttcttc gcccgaatta aatttggtat taactttagt accatccggt
      841 ttggaaatgg caacctcgcc acttttactt ttattgactt tgtcaagatt tttgttaata
      901 ccatcttgaa gtttgtcatc attttcattt tttgcaggtt gattggtttg ggcacctgct
      961 ccaagataag catttaaggc tttttcggtg agatcggctt tttgcttttt atctaaattt
     1021 ggattattgt taatggattt taatgcatta tctaaacgct tttccatcat tttttgcatt
     1081 tccaacttag ccgcttcttg accaaacatc tttgttgctt caaacgaagc tttgagtgct
     1141 tctaaggcat tggtttgatt ttgggctaaa ccggtaatat cgcggaatgc atcccccttg
     1201 cctaaaaggg aaagtgttcc tgccaaaccc gtaggatcgg gaacattggg tgcattttgt
     1261 atattaagca taggtgcagg caacgctgcg ggttgtaagt ttccaggttc agcacgacgt
     1321 gaatctgtat tgattggatt aatcgccgtg ggtgaatctg gaatgggcga ttcttcccaa
     1381 cgccaaaatc tactttcatc tttttcttca caagaattac atttgcccat catggcttcc
     1441 gcatacacac ctttggtggg aatactgata tttactggat cggcagcaga aaccatataa
     1501 taatcggtta gagagtcagc aagaccgaaa ttgggatcta aattcaatcc ttgcgccaca
     1561 gggaatacta agctattacc tacaattcca attaattcat tttcaactaa cgaagctaaa
     1621 cttcgcccta accctttttc tccgggtaag atgataccat ctaatagcat aaagcgacgt
     1681 tgttcggata attgcatcca aatggcggtg tggaaatatt ctaaattgtc gttaagatga
     1741 tcgataagct tattgaccag atgtaaatct tctttacgtg gatcttttaa ttcatcaccg
     1801 ctaggacctg cgtataacac taccccatca ctgggcgata aatcgtcatt tatcgcttgg
     1861 aatttacata aaaaaccttc atagtgtggc gttttataac ggataaaccc ggattgtaca
     1921 ttaatggaag aaaacgccgg caaggcattg gccgccccac aacgaatttt tatatagtga
     1981 attttatctc gactaaaagt ggatggtccc ataaaccgca ccgaaatatt taaggtacct
     2041 tctttgcgat agcgtgatgt aagcgatgaa tcaaagttca tagaggcaat ttcatttcct
     2101 gcttcatcgg ccacatgaaa ggttaattta tccacaaaag ataatgcaat tttctcgcct
     2161 aataacctgt gaaatacttc gtctttatta cgcgcatttt ttaaatgcat atcataaaaa
     2221 ccacgtgggg tatcggcccc catgaaagga attaaacttt gccaattgtc ttcattgaga
     2281 tcttctatat tctttttttg atagccaaca atcatgcctg cagggccata aattggcttg
     2341 gcatgatcgt ccgtttcaac gagtttatcg tctgggcgtc ggataataaa ttgtatttga
     2401 aagttacctg aagcatgcaa tattttttcg gatgcatagg ttccagaagg aaaattgcta
     2461 tcggtccatt cattttttac tctttgcgct gcttcaaaac ctttaacgag tgtattgtct
     2521 aaaagggtat tttgcagtgc ttctttccaa cgtagggttt tgtctaaatc gaattggccc
     2581 attaataaag gtactaataa acactcacgt gcgccagcaa agcgttgttt tactttaaaa
     2641 tggcgtaata cttcaaaata ttgaatggta atcgcatggc agtgattata attagcgatt
     2701 gtttcagagg acacttcaaa acgctcgcct tgcgctacag tctgaataac cgtggatctt
     2761 tgacttcgta ctgcatttgc agattgtact aatctgtcac gcactttttg taaatcactg
     2821 gcagaggttt gacgcaagct atcttgagag gcattcgatc cggagtgtcc tactccaccc
     2881 gaaactccaa acaataaccc ccccacaacg ccaccaatac ccgcacttgc gctggctgtg
     2941 gtggcactgg atttaccctt catcgattct tttattactc cttgggtaat ctcaaaaata
     3001 tcacgatcgc
//
LOCUS       DASTIB010000162         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_565417, whole genome shotgun sequence.
ACCESSION   DASTIB010000162 DASTIB010000000
VERSION     DASTIB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565417"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFP93_02770"
     CDS             complement(<1..630)
                     /locus_tag="VFP93_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9843562.1"
                     /translation="MKKFSAKKYCFLLFLWFIYSVNVQANVPVTVADVKTLIFYPTYE
                     VSASVLSLHNSLISSEIHGRVEILPFLVGDIVKEGSVIAKLDCTDQQYILEQVSNARD
                     GTKAKIEVAQWQLQQFDKLAKEQHVSTERLKTLASELQFLQAQQKIEDSKINQAKNHL
                     SKCSIKAPFNAIVTARSISLGEFASPGVPVMQLLSSDKLEVQAKLHPKEA"
     gene            930..1874
                     /gene="prmB"
                     /locus_tag="VFP93_02775"
     CDS             930..1874
                     /gene="prmB"
                     /locus_tag="VFP93_02775"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9843563.1"
                     /translation="MKNYPMDSKEATQELVTIRDFIRWAVSHFNQANLVFGHGTDNAW
                     DEAVHLVLSSLRLAPGSDQNVLEAKLTHTERKQIVINIERRVKERIPVPYLIQEAWFA
                     GLNFYVDERVIIPRSPIAELIEEEFSPWVESENVHHILDLCTGSGCIAISTALAIPHA
                     QIDAVDINQDALDVAAINVKKFAVQENVRLIQSDLFEKLEDKKYDIIVTNPPYVRTEE
                     YHFLPKEFHHEPKLALEAGEDGLQVVGRILQSAQNHLTPKGILIVEVGLLQENLEAKY
                     PDVPFTWLQFERGGEGVFLLTAEELKEFNYQLKQKRNS"
     gene            1871..2944
                     /gene="aroC"
                     /locus_tag="VFP93_02780"
     CDS             1871..2944
                     /gene="aroC"
                     /locus_tag="VFP93_02780"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004243753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9843564.1"
                     /translation="MTGNRFGRYFSLSSFGESHGTHIGGMLDGVPAGLAISETYIQAQ
                     MDRRKPGNSSFTSQRKEADKVAILSGILEGITLGTPIGFLIANTDAKPKDYTELQDVF
                     RPGHGDYVYQKKYGIRDSRGGGRASARETAVRVAAGAIAKTFLKAQFGIEIYGYLVQM
                     GEITIAKQNFNRDFIEQNPFFCPDPKLVDTLAENIHALRKNGESTGALIEVIAEGVPV
                     GLGEPVYHGLDADLAHAFMSINAVKGVSIGDGFTSVTQTGSYHRDQLNREGFLTNHAG
                     GILAGISTGQLIKAALAFKPTSSVPIPCNTLTHLEEEVIIRIKGRHDPCVGLRGVPIA
                     EAMMALVLADHAIARFGNKPIAN"
     gene            complement(2957..3391)
                     /locus_tag="VFP93_02785"
     CDS             complement(2957..3391)
                     /locus_tag="VFP93_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843565.1"
                     /translation="MGFLDKVKDKAEALKKEAQSMAQKAGQTATSAKERAQQMAGKGT
                     PKTTARAEDSTFSADLQNLYDKANKIESQSKELSQNAKLLRQSIQQLEQKIKQAFATV
                     STPTSPVGPEVQTEQVPPSPVIENEDAEQSNRKKKPQDVKKL"
     gene            complement(3402..3782)
                     /locus_tag="VFP93_02790"
     CDS             complement(3402..3782)
                     /locus_tag="VFP93_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843566.1"
                     /translation="MNTVLANNTDLEIWEGWVGDTHSIAATWYRQKNKAQCGFQVFST
                     WHGYFTLMLLHKDVWQTPVSRLQDPRKVRQLLFKYMSDQIAHVNRKGALTPQLEKSFQ
                     VAQESVNQWVNEQVKVLKWFVSYK"
BASE COUNT         1133 a          694 c          814 g         1223 t
ORIGIN      
        1 agcttcttta ggatgcagtt tggcttgcac ttctagttta tcggaagata ggagctgcat
       61 cacaggtaca cccggagaag caaattcccc aagagatatg gatcgagcgg ttacaatggc
      121 gttaaagggg gcttttatag aacatttact cagatggttt tttgcttggt taattttgga
      181 gtcttcgatt ttttgttgag cttgcaaaaa ttgtaattca gaagccaagg tttttaaacg
      241 ttcggttgat acatgctgtt ctttcgcaag tttatcgaat tgttgaagct gccattgggc
      301 gacttctatt tttgctttcg ttccgtctcg ggcattgcta acttgttcaa gtatatattg
      361 ttgatctgtg caatccaatt tagcaataac ggatccttct tttactatat cacccaccaa
      421 aaaaggtaag atttcaacgc gaccatggat ttcgctgctg atcaaagaat tatgcaagct
      481 taagacggag gcgctaactt cgtacgtagg ataaaaaatg agcgttttaa cgtcagcaac
      541 agttaccgga acattcgctt gcacgttcac gctgtaaata aaccataaaa aaagtaagaa
      601 gcagtatttc ttcgcgctaa attttttcat aagttatcat ttctcgaaac ttatttaaca
      661 aaatcaacta actattagca gttgaatttt ggaaagcaaa taaaccaaat ttaattatag
      721 tgatacgctt aggataattc aatattttta aataacatat tcataagtat ttttgtgaaa
      781 attatcacta atatcagaag taatgcattt attgcgcgaa tgcaataagt agcaggaatg
      841 cacctaatct tcctaagtac atgttgttgt agagtgcatc aatcagttac acttcgctaa
      901 aaatttttta agacagagca aaaaattaaa tgaaaaatta tcccatggat tccaaagaag
      961 caacacaaga gttagttacc atacgtgatt ttattcgctg ggcggtaagc cattttaatc
     1021 aggcaaattt agtatttgga catggtaccg ataatgcatg ggatgaagca gtgcatttgg
     1081 tgttgtcttc tttgcgctta gccccagggt cggatcaaaa cgttcttgaa gctaagctca
     1141 cgcataccga acgaaaacaa attgttatca atatagagcg tcgggttaaa gaacgcatac
     1201 ctgttcccta tttaatacaa gaagcctggt ttgctggatt gaatttttat gttgatgaaa
     1261 gagtaatcat tccacgatcg ccgattgccg aattaatcga agaagaattt agcccttggg
     1321 tggaaagtga gaatgttcac catatcttgg atctctgtac cggatcaggt tgtattgcta
     1381 ttagtacggc actagccatt ccacacgcgc aaattgatgc cgtagatatt aatcaagacg
     1441 ctttggatgt agccgccatc aatgttaaaa aatttgccgt acaagaaaat gttcgtttga
     1501 ttcaatccga tttatttgaa aaactggaag ataaaaaata cgatattatt gttacgaatc
     1561 caccctatgt tcgaacagaa gagtatcatt tcttaccgaa agaatttcat catgaaccca
     1621 aacttgcgtt agaagcagga gaggatggat tacaagtcgt gggtcgtatt ttacaatctg
     1681 ctcaaaatca tcttactccc aaaggaattt taatagttga agtagggctg ttgcaggaaa
     1741 atctagaagc aaaataccca gatgtacctt tcacttggct gcagtttgag cgtggaggag
     1801 agggagtatt tttgttaaca gcggaagaat tgaaagaatt taattaccaa ttaaagcaaa
     1861 aacgcaattc atgacaggca atcgattcgg cagatatttc tccctttcat cttttggcga
     1921 aagtcatggt acccatatag ggggtatgct ggatggtgta cccgcgggac tagctattag
     1981 tgaaacatat attcaagcgc aaatggatcg aagaaaacct ggaaattctt cttttacttc
     2041 gcaaagaaaa gaagcggata aagttgcgat tttatccgga attttagagg gaatcacact
     2101 aggtactcct attgggtttt tgattgccaa tacagatgca aaaccaaaag attatacaga
     2161 actgcaggat gtatttcgtc ctggtcatgg cgattatgta taccaaaaaa aatacggaat
     2221 acgcgattct cgaggaggag gacgcgcctc cgcgcgtgaa accgcggttc gcgtagcagc
     2281 aggtgcgatc gcaaaaactt ttttgaaagc tcaatttggt attgaaattt atggttatct
     2341 tgtgcaaatg ggcgagataa ctattgctaa acaaaatttt aaccgagatt ttattgagca
     2401 aaatcctttt ttttgtcccg atcctaaact tgtggatact ttagccgaga acattcatgc
     2461 cttaagaaaa aatggggaaa gtacgggggc tttaatagaa gttattgccg aaggtgtccc
     2521 ggttgggtta ggagaacctg tctatcatgg actggatgca gatttagccc atgctttcat
     2581 gtcgattaat gcggtcaaag gcgtaagtat tggggatggt tttacatcgg ttacgcaaac
     2641 aggctcgtat catcgagatc aattaaatag ggaaggattt ctcaccaatc acgcaggtgg
     2701 tatattagca ggcatttcta caggacaact tattaaagca gctttggcgt ttaagccgac
     2761 ctccagtgtt cctattccct gtaatacact cacacatttg gaagaagaag ttattataag
     2821 aattaaaggt cgtcatgatc cctgtgtagg gttgcgaggc gtgcctattg ccgaagccat
     2881 gatggcatta gtattagcag atcatgcgat cgcgcgtttt ggcaataaac ctattgcgaa
     2941 ttaaattttt tcaaagttaa agttttttaa cgtcttgtgg ttttttttta cgattggatt
     3001 gttcggcgtc ttcgttttct ataacagggc taggtggaac ttgctctgtt tgcacttctg
     3061 gtcctacagg acttgtcgga gtagaaacgg tagcaaatgc ttgttttatt ttttgttcga
     3121 gctgttgtat gctttgccga agcagttttg cattttggct aagctctttc gactgcgatt
     3181 ctattttatt tgccttgtca tatagattct gcaaatcagc ggaaaaagta gagtcttctg
     3241 cacgtgcggt ggtttttggt gttcctttgc ctgccatttg ctgagcccgt tctttagcgc
     3301 tggtggctgt ttgaccagct ttttgtgcca tactttgtgc ttcttttttt aacgcttccg
     3361 ctttatcttt tactttatct aaaaaaccca tgtattacct cttatttata gctcacaaac
     3421 cattttaaaa ctttgacttg ttcgttaacc cattggttca ccgattcttg ggcaacttga
     3481 aaagatttct caagctgcgg agttaaagca ccttttcgat taacatgagc tatttgatct
     3541 gacatatatt taaacaaaag ttgacgtact tttcgtgggt cttgtaggcg tgaaacgggg
     3601 gtttgccata cgtctttatg caataacatt aaggtaaaat acccatgcca agtactaaaa
     3661 acttgaaaac cacattgggc tttatttttc tgacgatacc aggtggcagc tatggaatgg
     3721 gtgtcaccta cccaaccttc ccatatttct aagtcggtat tatttgcgag tacggtattc
     3781 ataattgtaa aaattgcatg tttaatattg ctaggttatc gaagtcgatt aacttaataa
     3841 attggactta agcactagga gatg
//
LOCUS       DASTIB010000163         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1418013, whole genome shotgun sequence.
ACCESSION   DASTIB010000163 DASTIB010000000
VERSION     DASTIB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418013"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2106
                     /locus_tag="VFP93_02795"
     CDS             <1..>2106
                     /locus_tag="VFP93_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843567.1"
                     /translation="DEISLKQFFEQKYQAKKDEKWFIEQFVNAYVLYSNKSVPDQNMV
                     LQYSKVLAEISEKKTLRLQELKSSLKLRHHVANAVEYDAIYKKEEGTILEELNKEYET
                     MHNKFGTIFGVNLHDLTVQMGLSLVTDIGKINSVYYLRVKKEQLVKEILKKKILTEAF
                     QTETVVKGLSKKIDITMQLDMEKTARANIKRDFTKDTYLGLLAKPQRGKTLSLIVDEN
                     QRNIFIKNNEFDVMTENDLQEKMNPKAEHNGKKVLAQSFEFIPFLDFLAPSHVSQLAL
                     FFGNSYYTDENDFGGMFDPANSDHIEKLRKDLTIKLKNSRVTMADILLVALGLQFRSE
                     SIEEKFQLLRKNKYKNLEDLLGDQFFAKSPSEIIKVISEFAHLEPELIAQNDLVVRLT
                     MQEVKEQLPFLQEAFSQMEQNIKHTLREKIGCLGELSKIDIEANQAYGQFKKFLSMEL
                     FAKSILDYLEKAEDIYTVEGQRELLMASIKSSAVFKELKMAVINIQSEIKIVQQRIHA
                     KNAAMARISEVQANRDNSDYADIRQSVKIDLFEDVNIAESEKQKILIDKLARQMFQEK
                     YPKIALDPAEAILEEVKENENKIEKLQNKITIEKIHGSEEELQRLRDTVRMLEEENKL
                     LKLELEALQKIQGEFEQNKAELAKNKEQIEQNQQDLEKHKLLLANAQNDLLCFKRMFE
                     EQQEELILSTRYIEEQQEQL"
BASE COUNT          843 a          306 c          382 g          575 t
ORIGIN      
        1 gacgaaatta gtttaaaaca attttttgaa caaaaatatc aagcaaaaaa agatgagaaa
       61 tggtttattg aacaatttgt aaatgcctat gtgttatata gcaataagtc cgtgccagat
      121 caaaatatgg tgttgcaata ttccaaagta ttggctgaaa tttctgagaa aaagaccttg
      181 cgattgcagg aactcaaatc ctcattaaaa ctcaggcatc atgttgcaaa tgcggtagaa
      241 tatgatgcca tctataaaaa ggaagaaggt actattttag aggaattaaa caaagaatac
      301 gaaactatgc ataataaatt tggaactatt tttggagtca atcttcatga tttaacggtg
      361 caaatgggtt tgtcgcttgt caccgatatt ggcaaaatta atagtgtcta ctatttgcgt
      421 gtcaaaaaag aacaattggt taaagaaatt ttaaagaaaa aaatattaac cgaagcgttt
      481 caaacggaaa cagttgtaaa aggcttatca aaaaaaattg atattaccat gcaactcgat
      541 atggaaaaaa cagctcgggc taacatcaaa cgtgatttta caaaagatac ctatcttgga
      601 ttattggcta aaccacaacg tggtaaaaca ttatcattaa ttgttgacga aaatcagcgc
      661 aatattttca taaaaaataa tgaatttgat gtaatgactg aaaatgatct acaagaaaaa
      721 atgaatccca aagccgaaca caatggcaaa aaggtattgg cacaaagttt tgaatttata
      781 ccctttctag attttttagc acccagtcac gtctcccaac tagcgttatt ttttggtaat
      841 agctactaca cagatgaaaa cgattttggt ggcatgtttg atcctgcaaa ttcggaccat
      901 atagaaaaat taagaaaaga ccttactatt aagcttaaaa attcacgcgt tactatggca
      961 gatattttat tggttgcatt aggtctgcaa tttcgtagtg aatctatcga agaaaaattt
     1021 caattattgc gtaaaaacaa gtataaaaac cttgaggatt tactcggtga tcaatttttt
     1081 gcaaaatcac ccagtgagat tattaaagta ataagtgaat tcgcccatct tgagccagaa
     1141 cttattgcac aaaatgatct tgtagtaagg ttaaccatgc aagaagtaaa agaacagttg
     1201 ccatttttac aagaagcttt ttctcaaatg gaacaaaata ttaagcatac attgagagaa
     1261 aaaataggat gtttgggtga gctatccaaa atagacatag aagcaaatca agcttatggt
     1321 caatttaaaa aatttctttc aatggaactt ttcgccaagt caatactaga ttatttagaa
     1381 aaagcggaag atatttatac tgtcgaaggg caacgagaat tgctcatggc aagtatcaag
     1441 tcgagcgcag tctttaaaga acttaaaatg gctgtaatta acattcaatc tgagataaaa
     1501 atagtacagc aaagaattca tgcgaaaaac gctgctatgg cacgcatttc tgaagtgcaa
     1561 gcaaaccgag acaattctga ttatgctgat ataaggcaat ctgtaaaaat cgatttgttc
     1621 gaagatgtaa atattgcgga aagtgagaag caaaaaatac ttatagacaa acttgcgcga
     1681 caaatgtttc aagaaaaata tccgaagata gctctagatc ccgcagaagc tattttagaa
     1741 gaagtaaaag aaaatgaaaa taaaatagaa aaattacaaa ataagattac gattgaaaaa
     1801 attcatggtt cagaagaaga actacagcgc ctgcgagata cagtcagaat gctagaggaa
     1861 gaaaacaaac ttttaaagct tgaattagaa gcgttgcaaa aaattcaagg tgaatttgaa
     1921 caaaataaag cagagcttgc gaaaaataaa gaacaaattg aacaaaatca acaagattta
     1981 gagaagcata agctgttatt ggcaaatgcg cagaatgatc tactttgttt caaacgtatg
     2041 tttgaagagc agcaagagga attgatactt agtacacgat atattgaaga acaacaagaa
     2101 cagtta
//
LOCUS       DASTIB010000164         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1375619, whole genome shotgun sequence.
ACCESSION   DASTIB010000164 DASTIB010000000
VERSION     DASTIB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375619"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFP93_02800"
     CDS             complement(<1..243)
                     /locus_tag="VFP93_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9843568.1"
                     /translation="MRALVFHGSKDIRIDTVQDPTLQAPTDILLKVTATAICGSDLHI
                     YRGKVSGMKEGDILGHEIMGIVEETGKEVTHINKGDR"
     gene            612..>2059
                     /locus_tag="VFP93_02805"
     CDS             612..>2059
                     /locus_tag="VFP93_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843569.1"
                     /translation="MPQSIVVDTDNEPFLLHSAVLLEDLPKLQTLLTSEDINGKNKDG
                     DTPLHLAVTLNNLEVLQILLSSSTLNRNAKNNQGNSALLVALSKGNTDTIKFLLQDGG
                     FDLYEKNAFGPTLIHFAIECSDVEIVEAMITKGNIHLQNEMMVFNLLEKAFVRDKLEI
                     FNYFLSEVKSNPDFLPIICHFFRLAILRKKASFMNNLLAEAEIDLANNDFATFNMPIR
                     DNIQNILNTEGDIAKVADPKEIYIPKFLFPLGYKPVHVAAAIGDEYLLRQLVNKGNVD
                     VNALNHHGHTPLYQAAWKGNLGCVEYFLHLSSVNANPLTGFSLTANDFAAFKKAIQTE
                     NIDLIKQMLLFPEIRNALHIGSYLAAHNNVILRRATRYGDIEFVNFLLSHTEVLENVT
                     ANQNSALHIAQDNGHDLIELRLLQIPAVRNFDNLNLGQIAQHPENSTTNMYRNEQGLL
                     SSVKAYNKNIYDNLGRKVIFKDFIHYLESQYE"
BASE COUNT          671 a          376 c          334 g          678 t
ORIGIN      
        1 gcggtcacct ttatttatgt gagtaacttc ttttcccgtt tcttctacta tgcccattat
       61 ttcatgacct aaaatatctc cttctttcat tcctgaaact ttgcctcgat aaatatggag
      121 atcagaacca caaattgccg tggcagtgac ttttaacaag atatcggtag gtgcttgaag
      181 tgtggggtct tgaacagtat cgatacgaat atctttagaa ccatgaaaaa ccagtgctcg
      241 cataacattt ccctttttta gttaaaataa cgcacattct tatttaaatc gagactgaaa
      301 attttgacgg ccataaattt tatgttggct tgaacaaaaa gagtttgttg gtaagacatt
      361 ttgccacgtt atcttgaaaa ttcccccatg cgtccatttc tcttatttat ccaaacaatc
      421 atctagaaca ccatacgtga acaacattta ggttgctttt ttattaggat aaatacagtc
      481 gcaagaatag gtgtgccttt tgcaacaaac ccatgcttcc aacaataagg aaatacccac
      541 atatctaatt tatttttgtt cacgctttta ttttggtaaa ttatatggtc ttcctatttt
      601 ttggtatggt tatgccccaa tcaatcgttg tagatacaga taatgagcct tttcttctcc
      661 atagtgcagt cttactcgaa gacttaccca aattacaaac actgttaacc tccgaagata
      721 ttaatggtaa aaataaggat ggtgacaccc ccctgcattt ggccgtcaca ttaaataatc
      781 ttgaggttct tcaaattttg ttgagttcat ctaccttgaa taggaatgct aaaaacaatc
      841 aaggaaattc tgcacttttg gttgcacttt ctaaaggtaa cacggatacc attaaatttt
      901 tattacaaga cggcggtttt gatctttatg aaaagaacgc ttttggcccg accctaatac
      961 attttgctat tgaatgctcg gatgtggaaa tcgttgaagc catgataaca aaaggaaata
     1021 ttcatcttca aaacgaaatg atggtattta atttactaga aaaagcattt gtacgagaca
     1081 agctagaaat ttttaattat tttttaagtg aagtaaaaag taatcctgac ttcttaccca
     1141 taatttgcca tttttttcgt cttgctattt tacgtaaaaa agcctccttt atgaataact
     1201 tattagcaga agctgagatc gatcttgcta ataatgattt tgcaaccttc aacatgccta
     1261 tacgcgacaa tattcaaaat attttgaata cagaaggaga tattgcaaaa gtagcagatc
     1321 ccaaagaaat atatattcct aaatttctct ttcctttagg ttataagcct gtgcatgtgg
     1381 ctgctgccat cggcgacgaa tatttacttc gccaactagt taacaaagga aatgtcgatg
     1441 ttaatgcgtt aaaccatcac ggtcatactc cattgtatca ggcagcctgg aaaggaaatc
     1501 ttggatgtgt tgagtatttt ttacacctat cttctgttaa cgcaaatcct ttaacgggtt
     1561 tcagtcttac tgcgaatgat tttgcagcct ttaaaaaagc gatacaaact gaaaacatcg
     1621 acttaataaa acaaatgctg ctttttccag agattcgaaa cgcccttcac attggttcct
     1681 atttggcagc acacaataat gtaattttaa gaagggcaac caggtatgga gatatagaat
     1741 ttgttaattt tcttttaagt catactgaag tgttagaaaa tgtaactgct aatcaaaata
     1801 gtgcattaca tatagcacaa gataatggcc acgatttaat tgaattgcgt ttattacaga
     1861 tcccagctgt gagaaatttt gacaatttaa atttaggaca aatagctcaa catcctgaaa
     1921 attcaacaac gaatatgtat cgcaatgaac aaggtttatt gtcctcagta aaagcttaca
     1981 acaaaaacat ctatgataac ttgggaagaa aagtaatttt taaagatttt attcattatc
     2041 tcgaatcaca atacgaatt
//
LOCUS       DASTIB010000165         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_134334, whole genome shotgun sequence.
ACCESSION   DASTIB010000165 DASTIB010000000
VERSION     DASTIB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134334"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFP93_02810"
     CDS             <1..190
                     /locus_tag="VFP93_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9843570.1"
                     /translation="FWADMLAWFKKKLVGEGMISEDDLKLYSLANNPEEVVKTLQEFY
                     KDRSFEPSPREREIMSDL"
     gene            353..1690
                     /locus_tag="VFP93_02815"
     CDS             353..1690
                     /locus_tag="VFP93_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843571.1"
                     /translation="MKNRHAFSQKQILNLLRLGKKFIDFEATELFRGWPTDPGTELID
                     NIVLPIADMNISGSASTKLVEEALTVARTKESIEPFIKKLAVHQEKFSPEKWEALIGT
                     LHNPKLKDVTIPSSDWTDVIKRAIMHGKILDQDSIDVRAVQALLEEELISIFAPAFNG
                     LYRDHPQDEVTRKPNFFMELVNGPFEDPAQLGQPIERFLVRLAKLDKKKSAAIIHDFI
                     YFAKKATEKSEATTDGMANMLNVALMSGLELSNSFTLPESYADKDDVHAMNRIAYRFM
                     AAVALSCLQHVTDKMDPSSSSSTLEPSLLFTKSFHEQETYYQNKAVDLEAVKGKKQEF
                     IQMLWNHPPQAKNSELTSKSNGQEVVASSSKQVDKKQKGKDRRIERPHSMPINAKNLG
                     SRSEPAITHVGESKQNTLLQNYNASTSNDKVVERKSMKKEKKKKNILKRVFKK"
     gene            complement(1748..2527)
                     /gene="mazG"
                     /locus_tag="VFP93_02820"
     CDS             complement(1748..2527)
                     /gene="mazG"
                     /locus_tag="VFP93_02820"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET9843572.1"
                     /translation="MTNANIFDELIAMLQQLRDPSTGCPWDLQQTLESIVPHTIEEAY
                     ELAHAIEHKNDDETTHELGDLLLQILFLAQIGSEEQKFTILDICVALKRKLIQRHPHV
                     FGNEHVKNAQEQALLWEKRKLTERKNIFAGIPHNLPALSRAQKIQKRVATVGFEFKSM
                     QDAFFKIQEEVLEIQKELEAGLLVKAQQELGDLLFACCNLARFLDKDAEQLLRQATEK
                     FERRFAWVEDKVQQSGKTWNETHVTDLEMFWQQAKRNLQNL"
     gene            complement(2524..>4396)
                     /locus_tag="VFP93_02825"
     CDS             complement(2524..>4396)
                     /locus_tag="VFP93_02825"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET9843573.1"
                     /translation="TQGVMHLDHTQNKHAIRQKNISISADSSDNLRRMLLAVIEDIRV
                     VIIKLAERIAALRSCTDVENEACKNIALEAQEIYSPLANRLGIGQIKWEIEDLAFRIL
                     EPLAYKQIAKALDEKRLDREEYILKIIDQVTQALNREGIQAHVKGRVKHIYSIWRKMQ
                     RKNLDFNEIYDVRAIRIVVPKIQDCYAALGVVHSLWQHIHKEFDDYIATPKENGYRSL
                     HTAVIGPFGKTLEIQIRTGEMHQEAELGVAAHWVYKEEKKQDARYQSKLSAIRQLLEE
                     SAEFSDQKEYQSLVHSELQEDRVYVFSPMGDVLDLPRGSTPIDFAFEIHTQLGYRCRG
                     AKVNGVMVPLTHQLKSGDQVEILSAKREGPSRDWLNTNLGFLKSTKALAKVHAWFKKQ
                     KRDENIEQGREILEREMRRLDLEKSNLQKVAVKLNFKAVEDLFAAIGSGDTKLGHVLN
                     TFQTPEPIKQEIPVSRISRIKDANVKIQGIGNLMSSFAKCCKPVPGDRIVGYLTVGRG
                     VSIHRQDCLNVLEVYPDQRVIQVEWMGISQSVFPVDIEIHALDRHDLLRDITSIISHE
                     KINVTGISSKTHPNEEAVKIIVNIQVQNLELLARILNKILQLPNIIEAKRVTLESKK"
BASE COUNT         1331 a          862 c          787 g         1416 t
ORIGIN      
        1 tttttgggca gatatgttag cttggtttaa gaaaaaactg gttggagagg ggatgatttc
       61 tgaagacgac ttaaagcttt actcacttgc caataatcct gaagaagtcg tcaaaacttt
      121 acaagaattt tataaagatc gcagttttga accttcccca cgtgaacgag aaattatgtc
      181 tgatttgtaa aaggcttcaa gccttttacc ttttttgaca tgtaactaca ctaatttttt
      241 tcaggttttt cccttttttc ctctaacact acttataatg ctgataaaaa gcgcaacctt
      301 aatttaaaat gcctgtcata taatggtatt acttatagta tggtaggcta acatgaagaa
      361 tagacacgct ttttctcaaa aacaaatttt aaatctttta cgattaggta aaaaatttat
      421 agattttgaa gcaactgaat tatttcgtgg atggccaact gaccctggta ccgagttaat
      481 cgacaacatc gttctgccta tcgcagatat gaatataagc ggctctgcaa gtactaaatt
      541 ggttgaagaa gcactgaccg ttgctcggac caaagaaagc atagagcctt tcattaaaaa
      601 actagcagtt catcaagaaa aattttcacc ggaaaagtgg gaagcattga taggaacttt
      661 gcataatccg aaactgaaag atgttaccat tcctagttca gattggacgg atgtaattaa
      721 aagagccatt atgcatggga agatattaga tcaggactcc atagacgtaa gggcagttca
      781 agctttactt gaagaagagc ttataagcat ttttgctccc gcgtttaatg gtttataccg
      841 agaccatccg caggatgagg taaccagaaa accaaatttt tttatggagt tagtaaatgg
      901 cccttttgag gatcctgctc aattaggtca accgatcgaa agatttttag ttcgattagc
      961 gaaactcgat aagaaaaaaa gtgctgcaat catccatgat ttcatctatt ttgcaaaaaa
     1021 agctacagaa aaatcagaag caacaacgga tggaatggca aatatgctca acgtcgcttt
     1081 aatgtctggc ttggaattga gcaattcttt tacgctacca gaaagctatg ccgataaaga
     1141 cgacgttcac gctatgaatc gcattgctta tcgatttatg gcagctgtag cattatcttg
     1201 cttgcaacat gttacggata aaatggatcc aagtagcagt tcaagtacgc tcgaaccttc
     1261 acttttattt acaaaatcgt ttcatgaaca agaaacttac taccaaaata aagcagtaga
     1321 tcttgaggca gtaaaaggga agaaacaaga atttattcaa atgctctgga atcatcctcc
     1381 ccaagctaaa aatagtgaac taacttccaa atccaatgga caagaagttg ttgcttcttc
     1441 ttcgaaacaa gtagataaaa aacaaaaagg caaagacaga agaatcgaac gtcctcattc
     1501 tatgcctatt aatgcaaaaa atttaggctc acgctcagag cctgcgatta cccatgtggg
     1561 ggaatccaag caaaacacac ttctacaaaa ctataatgct agcactagca acgacaaagt
     1621 ggttgagcgt aagagtatga aaaaagaaaa gaagaaaaag aatattctaa aaagagtatt
     1681 taaaaaataa ttttaaatta ccatgtaaag ctgcccgggg gcagcttttt ttataaaaaa
     1741 actactttta taaattttga aggtttcttt tagcttgttg ccaaaacatt tccaaatccg
     1801 ttacatgggt ttcattccat gtttttccac tttgttgaac tttatcttca acccatgcaa
     1861 aacgtcgttc aaacttttcc gtagcctgac gtaacaattg ctcggcatct ttatcgagaa
     1921 atctagctaa attacaacaa gcaaataata aatcacctag ctcttgttgc gctttgacta
     1981 agagacccgc ttcaagctct ttttgtattt ctaagacctc ttcctggatt ttaaaaaaag
     2041 catcctgcat ggacttaaac tcaaatccta cagttgcaac gcgtttttgt attttttgtg
     2101 cgcgtgataa agcgggtaag ttatgaggaa tgccagcgaa aatatttttg cgctccgtta
     2161 atttcctttt ttcccataat aatgcttgct cttgggcatt tttaacgtgt tcattaccaa
     2221 aaacatgggg atgtcgttga attaacttgc gttttaaagc tacacaaata tccagaatag
     2281 taaatttctg ttcttcactt cctatttgcg ctaaaaataa aatttgtaaa agcagatcgc
     2341 ctaattcatg ggttgtttca tcatcgtttt tatgctctat ggcatgcgct aattcataag
     2401 cttcttcgat agtgtgtggc acaatcgatt caagggtttg ctgcaaatcc caggggcatc
     2461 ctgtggaggg gtcgcgcaat tgttgaagca tagcaattaa ttcatcaaaa atatttgcat
     2521 tagtcatttt ttggattcta gtgtaacacg ttttgcttct ataatatttg gcaattgtaa
     2581 tattttattt aagatacggg caagtagttc taaattttgc acctgaatat ttacaataat
     2641 cttaacggct tcttcgtttg gatgtgtttt tgagcttata cccgttacat tgattttttc
     2701 atgagaaata atgctggtaa tatctcttaa taaatcgtgg cgatccaagg catggatctc
     2761 gatatccacg gggaacaccg attgtgaaat ccccatccac tcgacctgaa ttacacgttg
     2821 atccggataa acttcaagta cgtttaggca atcttgtcga tgaatagaaa ctcctctacc
     2881 aaccgttaaa taaccgacaa tgcgatcccc aggtaccggt ttgcaacatt tagcaaacga
     2941 gctcattaaa tttcctattc cttgaatttt tacatttgcg tctttaatac gtgatattct
     3001 ggaaacagga atttcttgct ttatgggttc tggtgtttga aaagtgttta atacatggcc
     3061 taattttgtg tcgccactcc caatagcggc aaataaatct tctaccgctt taaaatttaa
     3121 cttaaccgcc actttctgta aattggattt ttccaagtct aagcggcgca tttctctttc
     3181 cagtatttcg cggccttgtt cgatgttctc atcccgtttt tgttttttaa accaagcatg
     3241 cacttttgcc aatgctttgg tactttttaa aaaacctaag ttggtattta accaatctcg
     3301 cgagggtcct tcacgtttcg cgcttaatat ttctacttga tcccccgatt tcaactggtg
     3361 tgtcagaggt accataaccc cgttcacttt tgcaccccga cagcggtatc ctaattgggt
     3421 atgaatttca aatgcaaagt caatgggggt agagcccctt ggcaaatcaa ggacatctcc
     3481 catgggcgaa aatacataca ctcggtcttc ttgtagctca gaatgcacca aactttggta
     3541 ttctttttga tcggaaaatt ctgcactttc ttctaataat tggcgtattg cgctgagttt
     3601 agattggtac cgcgcatctt gcttcttttc ttctttataa acccaatggg ctgccacacc
     3661 taactcagct tcttgatgca tttctccggt acgtatttga atttccaagg ttttaccgaa
     3721 agggcctatt acggcggtat gtagagaacg atagccattc tccttaggag ttgcaatata
     3781 atcatcgaat tctttgtgaa tgtgttgcca taaagaatgt acaacaccta aggctgcata
     3841 acaatcttgt attttgggta ccacaatacg gatggctctt acatcataaa tttcattaaa
     3901 atctaaattt ttacgctgca ttttacgcca aatactatag atatgcttta cgcgaccctt
     3961 aacatgcgcc tgaatgcctt ctctgtttaa ggcttgcgtt acttgatcaa taattttaag
     4021 aatatattct tcgcgatcta aacgtttttc atcgagagct tttgcaattt gtttataagc
     4081 caaaggctca aggatgcgaa aagctagatc ttctatttcc catttaattt gaccgatgcc
     4141 aagtcgattc gctaaaggcg aataaatttc ttgtgcttcc aatgcaatat ttttacaagc
     4201 ttcattttct acatccgtac aactacgaag tgctgcaatt ctttctgcta gtttaattat
     4261 gacgacacga atatcttcaa ttaccgctaa aagcattcgc cgtaaattgt cactgctatc
     4321 tgcactaatt gaaatatttt tttgtcttat tgcatgctta ttttgggtgt gatcaagatg
     4381 catcacacct tgtgtc
//
LOCUS       DASTIB010000166         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1371649, whole genome shotgun sequence.
ACCESSION   DASTIB010000166 DASTIB010000000
VERSION     DASTIB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371649"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFP93_02830"
     CDS             <1..508
                     /locus_tag="VFP93_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9843574.1"
                     /translation="PELEKQLVGVVFLNTAPDNYWYRSFAERSKQQDLPNVSLFRKQL
                     LEHPCNAHYAEYLLQSLRYYFREAFLEQGKQLFLTLPFNYKPYFWAIEHFHPTYQASW
                     IPAMPCLIVGSEFDVMTPPDVFEQTPYRANNFRHVTISNAAHFPWIENLSAVQVHLQD
                     FEKVILNA"
     gene            complement(498..1280)
                     /gene="cysZ"
                     /locus_tag="VFP93_02835"
     CDS             complement(498..1280)
                     /gene="cysZ"
                     /locus_tag="VFP93_02835"
                     /inference="COORDINATES: protein motif:HMM:NF003433.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HET9843575.1"
                     /translation="MKGLVFSHLLKGYRLLWQPGFKKYLFIPLALNIIIFLSLTTLSL
                     YYTWHILSNFQFTLPSWLHWTQSWLPYLGTFLFWIGWIGSMLFILIAIVPVAILLTNL
                     LASPFNSLLCEKILKDFGSTPIPPSSWRTVVKEAFLRELQKMLYFLPRMGVLFLLFAV
                     CTFIPIINVLAAGLFYVCSSWLLSLEYLDYPGETLRFTFHEVKKHTTKKRWVRFHFGL
                     SAMLFSTVPLLNLMCIPVCVAAASLVWIDSFESRKENHTVKR"
     gene            complement(1277..2305)
                     /locus_tag="VFP93_02840"
     CDS             complement(1277..2305)
                     /locus_tag="VFP93_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9843576.1"
                     /translation="MRDPILDKLHYPDFKSVPIFSSGKAQTLLTFFYPALFDIPPTEI
                     HALTLPDDDKLVLMEYKPSTWRPGKRIIVLVHGLTGSFNSKYNVRLCRLWMKVGFMVI
                     RLNLRGCGPGTGLARHPYNSGRSEDTRFVVRWLYERYPNSPVTQVGFSLGGNITLKMA
                     AEDGKYPAGNLDSIIAVSPPVDLHACSALLHKPENRVFEKYFVQTLIKDYHTRRSHFP
                     DLPELQLDPNMALLDFDNLYTAPYSGFKDAIDYYTQCSSGMILDKIKLPTFILYAKDD
                     PFITIERFMYKMKTANFDTLVTTTGGHLGWLGSFLLGTNRHWMDGVLVRWLAWFLSHQ
                     KSTIATTS"
     gene            complement(2347..>2718)
                     /locus_tag="VFP93_02845"
     CDS             complement(2347..>2718)
                     /locus_tag="VFP93_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9843577.1"
                     /translation="AYSIYKNLKPGGKFVGINDNPGNAPDYYKKYKKYGFLKSGQKQR
                     QEGSSIIYTFFKEDGSTYSLQSYYLSLETYEKVFLSVGFKDFRWKTLSISEKSKEKYN
                     LDYWRIFLNHPPIIGIEAIRD"
BASE COUNT          897 a          517 c          520 g          784 t
ORIGIN      
        1 gccggaactt gaaaaacagt tggttggtgt tgttttcttg aatacggccc cggacaatta
       61 ttggtatcga agttttgcag aaagatcaaa acaacaagat ttgcccaatg tatccttgtt
      121 taggaaacaa ctattagaac acccttgcaa tgcacactat gctgaatatt tgttgcaaag
      181 tttgcgttac tattttcggg aagcattttt ggagcaagga aaacaattat ttttaacact
      241 tccttttaat tataaaccct atttttgggc aatagaacac tttcatccaa cctatcaagc
      301 gagttggatc cccgcaatgc catgtcttat tgtaggatcc gagtttgatg tgatgacacc
      361 acccgatgtt tttgagcaaa caccctatcg cgcaaacaat tttagacacg ttacgatttc
      421 taacgctgca cattttccct ggatagagaa tttgtctgca gttcaagttc atttacagga
      481 ttttgaaaaa gtaattctta acgcttaact gtatgatttt ctttccggct ctcaaaagaa
      541 tcgatccaaa ctaaactagc tgcagcaaca cacaccggaa tacacattaa attcaacaga
      601 ggaaccgtgg agaagagcat agcgcttaaa ccaaaatgga atcggaccca ccgctttttt
      661 gtagtgtgtt tttttacttc gtgaaacgta aaacgtaaag tctctcctgg ataatctaaa
      721 tactctaaac taagcaacca gcttgaacac acataaaata atcccgctgc taatacgtta
      781 ataatgggaa taaaggtgca aactgcaaat agtaaaaata aaacacccat tctgggtaaa
      841 aaatagagca ttttttgcaa ttcgcgcaag aaagcttctt taaccacggt gcgccacgag
      901 cttgggggta tgggagtact gccaaagtct tttaaaattt tttcacacaa taaactattg
      961 aaaggagaag caaggagatt ggttaataaa attgccacag ggacgatcgc gattaaaata
     1021 aagagcatgg atcctatcca accgatccaa aataaaaatg tgcctaaata gggtagccaa
     1081 ctttgggtcc aatgcaacca actgggtaag gtgaattgaa agtttgaaag aatatgccaa
     1141 gtgtaataca aactcagtgt ggtaagactt aaaaaaataa taatattaag ggccagggga
     1201 ataaaaagat attttttaaa cccaggttgc cacaataatc gatagccttt caggagatgc
     1261 gaaaatacca aacctttcat gaagtcgtag caatggtgct tttttgatgt gatagaaacc
     1321 acgctaacca tcggaccaac acgccatcca tccaatggcg attggttcct aaaagaaatg
     1381 aacccagcca acccaaatgt ccgccggtag tggtgactaa ggtatcgaaa ttggcagttt
     1441 tcattttata cataaagcgt tcaatggtga taaacggatc atctttagca taaagaataa
     1501 acgtgggtag tttaattttg tctaatatca taccggatga gcattgcgta taataatcga
     1561 tagcatcttt aaacccagaa tagggagccg tataaagatt atcaaaatcc agcaaagcca
     1621 tattgggatc caactgtagt tccggcaagt ctggaaaatg gcttcttcgc gtgtggtaat
     1681 ctttaattaa cgtttgcaca aaatattttt caaaaacgcg attttcgggt ttatgtaaaa
     1741 gtgccgagca agcatgtaaa tctaccggtg gtgatactgc tataatgcta tctaaattgc
     1801 cagcaggata ttttccatct tctgctgcca tctttaaggt aatatttccg cctaaggaaa
     1861 agccaacttg tgtaaccggc gaattcggat agcgttcata gagccagcgc acgacaaagc
     1921 gtgtgtcttc gctacgtccg ctattgtaag ggtggcgcgc caaacccgtg cctggaccgc
     1981 aaccgcgcaa attaagtcga ataaccatga aacccacttt catccataat cgacaaaggc
     2041 gcacattgta tttggaatta aaagaaccgg ttaaaccatg tactaaaaca atgatccgtt
     2101 ttcctggacg ccaggtagaa ggtttatatt ccatcaatac gagtttgtca tcgtccggga
     2161 gcgtgagagc atgaatttcc gtcggcggta tatcgaatag agccgggtaa aaaaaagtaa
     2221 gaagggtttg tgctttgcca gaggaaaaaa taggtacaga tttaaaatct ggataatgca
     2281 gtttatctaa aataggatct ctcattattt ctatatttta gcacagcaat aaaaacatac
     2341 aaccatctag tcgcgaatgg cttcaatgcc aataatcggt ggatggttta aaaaaattcg
     2401 ccaataatct aagttatatt tttcttttga tttttctgaa attgacaagg ttttccaacg
     2461 aaaatcttta aatcctacgc ttaaaaaaac tttttcataa gtttctaggc ttaaataata
     2521 actttgtaaa gaatacgttg aaccgtcttc cttaaaaaag gtataaataa tagaacttcc
     2581 ttcttggcgt tgtttttgtc cagattttaa aaaaccatat tttttatatt ttttgtagta
     2641 atcaggagca tttccgggat tgtcgtttat acctacaaat tttccgccgg gtttcaagtt
     2701 tttataaatc gaataagc
//
LOCUS       DASTIB010000167         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_118099, whole genome shotgun sequence.
ACCESSION   DASTIB010000167 DASTIB010000000
VERSION     DASTIB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118099"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /gene="gyrB"
                     /locus_tag="VFP93_02850"
     CDS             complement(<1..1689)
                     /gene="gyrB"
                     /locus_tag="VFP93_02850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET9843578.1"
                     /translation="MTSQSTIYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVF
                     EVVDNAVDEALAGFCTLIEVIIHSDESVTVKDNGRGIPVDIHTEEGRSAAEVIMTVLH
                     AGGKFDDNSYKVSGGLHGVGVSVVNALSEELKLTIRRNGKIYQQHYRLGDPQHPLAAT
                     GETDKTGTDVWFKPSATIFSNIEFHYDILAKRLRELSFLNSGVRIKLFDERSGKRDEF
                     CYEGGIRAFVEYLNKKKTPIHPTVLYYNGTKDLVQIEIALQWNDSYQENIYCFTNNIP
                     QKDGGTHLSGFRSALTRTLNHYIEQEGVAKKDKIQTTGDDSREGLTAVLSVKLPDPKF
                     SSQTKDKLVSSEVKGAVEHLFGEMLNNFLMERPSEAKAIVHKVMEAARAREAARKARE
                     MTRRKGALDIAGLPGKLADCQERDPANSELYLVEGDSAGGSAKQGRDRRTQAILPLKG
                     KILNVEKARFDKMLSSQEVGTLITALGCGIGREEYDPSKIRYHRIIIMTDADVDGSHI
                     RTLLLTFFYRQMPELIERGYLYIAQPPLYRVKRGKQESYVKDDEGLREFLTTIAL"
BASE COUNT          600 a          443 c          369 g          649 t
ORIGIN      
        1 taaggcaata gtcgttaaaa attcccgcaa accttcatca tcttttacgt agctttcttg
       61 ctttccgcgt tttacgcgat acagcggtgg ttgtgcaata tataaatagc ccctttcgat
      121 taattccggc atttgacgat aaaagaaggt taataataaa gttcgtatat gtgatccgtc
      181 gacgtcagca tcggtcatga taataatgcg atgataacga attttcgaag gatcgtattc
      241 ttctcgtcca atcccacaac ctaaggcagt gataagagtg ccaacttctt gagaggaaag
      301 cattttatcg aaacgcgctt tttcgacatt taatatttta ccttttaacg gcaaaatagc
      361 ttgggtgcgt cgatcacgcc cttgctttgc cgaaccaccc gcggaatcac cctctaccaa
      421 ataaagttca gaatttgcag gatcgcgttc ttggcaatca gccagctttc caggtaaccc
      481 tgctatgtct aaagctccct tacggcgagt catctctcga gcttttcttg ccgcttccct
      541 agcacgcgca gcttccataa ctttatggac aattgctttt gcttcactgg gtctttccat
      601 taaaaagtta tttaacattt cgccgaataa atgctcaact gctcctttaa cttcggaaga
      661 taccaactta tctttggttt gtgatgaaaa tttaggatcg ggtaatttaa cagaaagtac
      721 cgcagtcaaa ccttctcgcg aatcatcacc ggttgtctga attttgtctt ttttcgctac
      781 accttcttgt tcaatgtagt gatttaacgt acgtgttagt gcggatcgaa atccagaaag
      841 gtgcgtgcca ccatcttttt gtggaatatt attagtaaag caataaatgt tttcttgata
      901 agaatcattc cattgtaagg caatttcaat ttgaacgaga tctttggtgc cattgtagta
      961 aagcaccgta gggtgaatag gtgttttctt tttatttaaa tactcaacaa atgctcgaat
     1021 accaccttca taacaaaatt catcgcgctt ccctgagcgc tcatcaaaga gcttaattct
     1081 aacgcccgaa ttcaaaaacg aaagctctcg aagcctttta gccaaaatat cgtaatggaa
     1141 ttcaatattt gaaaaaatgg tagcgcttgg tttaaaccaa acatcggttc ccgttttatc
     1201 cgtttctcct gtagcagcaa gaggatgctg cggatcacct aaccgatagt gctgttgata
     1261 tatttttcca ttacgccgta tggttagttt taattcttcg gataaagcgt ttacaaccga
     1321 aacaccaaca ccatgcaaac cgccggaaac tttataagag ttgtcgtcaa atttccctcc
     1381 agcatggaga acggtcataa taacttctgc agctgagcgt ccctcttctg tatggatatc
     1441 cacaggaatt ccacggccat tgtctttgac agtaacagat tcgtcagaat ggataattac
     1501 ttcaataagg gtacaaaagc cggccaaagc ttcatccaca gcgttatcca caacttcaaa
     1561 taccatgtgg tgcaaaccag taccatcgtc cgtgtcgccg atatacattc ccggcctttt
     1621 tcgtacagca tcaaggccct tcagaacttt tatactcgaa gaatcgtaga tagtgctttg
     1681 tgaagtcata taggctccag cgataaattc aaaatagctt tagaggagag tgagggaagg
     1741 cttttatgcg ctttccacaa ataatttttt ttcttggcta ttttaacata tatcaatact
     1801 ttccaccgct tattttcggc aaagcgtacg agcacatcga tagaaaggaa tgtaatgctt
     1861 taaaataatt agataaaagc cttaaaagaa tgaggatgat ttttaacaaa aattaactac
     1921 acctttcaac tacttattaa cttagttatc cacaggtgta tccacaatag tgtgatctat
     1981 atccttcatt tttaatactt tttttaaaag cgcaaaatta ttagtttttc tatccacatt
     2041 ttttggattt gcattaaatg a
//
LOCUS       DASTIB010000168         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_508322, whole genome shotgun sequence.
ACCESSION   DASTIB010000168 DASTIB010000000
VERSION     DASTIB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508322"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1269)
                     /locus_tag="VFP93_02855"
     CDS             complement(139..1269)
                     /locus_tag="VFP93_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET9843579.1"
                     /translation="MHAKAGGLAGIKAGSTAICTVGKEGKGLHYRGYSIYDLCENSLF
                     EEVAYLLIYGELPNQGQLTRYQLTLEANRNLPSVLKSALELLPSTAHPMDVLRTICSF
                     LGSLEPETEFHQGRAIADRFLALFPGALLYWHHFHRHGVRLNFEVKAQTLAEYFLTLL
                     HGSKLEKFPHKQLMIETLNISLILYAEHEFNASTFAARVCTGTRSDFYSAMCSAIGTL
                     RGPLHGGANEKAMFLIEEFNNVEDAEKGVVNKLARKELIMGFGHRVYSESDPRSDIIK
                     QKSKQLSTALEDKILYPISERIEKVMWNEKKLFPNLDFYSATAYHFLGIPTEMFTPLF
                     VLARTAGWAAHILEQRADNKLIRPSAEYIGPQPRSLPALAQR"
     gene            complement(1269..2156)
                     /gene="prpB"
                     /locus_tag="VFP93_02860"
     CDS             complement(1269..2156)
                     /gene="prpB"
                     /locus_tag="VFP93_02860"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HET9843580.1"
                     /translation="MSQKLIPLLREAISVEKPLQLIGVINAYCALLAEQLGFKALYLS
                     GAGIANADLGIPDLGMTSLEDVLSQVRRITSRISLPLVVDADTGFGSSLNIYRSFYEL
                     SKAGASGAHIEDQKSAKRCGHREGKKLVSKEIMGSRLKAALDGRYTEDFMVIARTDAL
                     SVEGVEKTLERACYYQDIGADMLFLEAANDLAIYQTFTKTLRIPVLANITEFGQTPLF
                     NLDQLKSVGVGVALYPLSVFRAMNFAAIEVLKCIRNEGTQKSVLNKMQTRDELYDTLD
                     YINFEKKIDKYLENVQGEE"
     gene            complement(2258..>2634)
                     /locus_tag="VFP93_02865"
     CDS             complement(2258..>2634)
                     /locus_tag="VFP93_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843581.1"
                     /translation="QELEPALIRADKLLENILERQDVIKKIPQSTQSMRHELDTLLSM
                     QGDLENRKKQLLTETQVLFKALHYKNANFTHPRMQELNQVKDVRHTKNFTPLQTMNTK
                     NKSDQKEEKKYVYKSKIAPKNL"
BASE COUNT          755 a          528 c          469 g          882 t
ORIGIN      
        1 ataatgtgcc gtctcatagg caagttcaga gttaattgtg gtatccatta tataatcagc
       61 gataagaaca agttcgctat cgtattcggg gcgattattg tctaaactca cttgcattaa
      121 actttctcca aatggaagtt atctttgagc caacgcaggc aaacttcgcg gttgtggtcc
      181 aatatactct gcagaaggtc gaatgagttt attatccgcg cgttgctcca agatgtgtgc
      241 cgcccatcct gcagtacgtg cgagcacaaa tagaggtgta aacatttccg taggtattcc
      301 taagaaatga taagctgtag cactataaaa gtctaaattt ggaaataatt ttttttcatt
      361 ccacattact ttttcaattc tttcgcttat cggataaagt attttatctt ctagtgctgt
      421 acttaattgt ttagattttt gcttaatgat atcggatctc ggatccgatt cagaatatac
      481 gcgatgacca aatcccataa taagttcttt acgtgctaat ttatttacta cgcctttttc
      541 tgcatcctct acgttgttaa attcttcaat taaaaacatg gctttttcgt tagctccccc
      601 atgtaagggg cctcttaacg ttcctatcgc tgagcacatg gccgaataaa aatcggaacg
      661 tgttcctgtg cacacgcgcg ctgcaaaagt agaagcatta aattcatgct ctgcatacaa
      721 aattaaagaa atattcaatg tttctatcat caattgttta tgaggaaact tttcaagttt
      781 tgaaccgtgc aataaggtta aaaaatattc tgcaagggtt tgtgccttca cctcaaagtt
      841 tagtcgcaca ccatgcctgt gaaaatgatg ccaatacagt aatgcgcctg gaaataaagc
      901 aagaaatcga tccgcaatcg ctcgaccctg atgaaattcg gtttcaggtt ctaaagatcc
      961 taaaaaagaa caaatagtcc gaaggacatc catggggtgt gcagtcgatg gcaacaactc
     1021 taaagcagat tttaaaacac tagggaggtt acgattcgct tctaaggtta attgataacg
     1081 tgtaagttgt ccttgattcg gtagttctcc ataaattaat agatacgcta cttcttcaaa
     1141 taaagaattt tcacagagat cataaataga ataacctcgg taatgtaatc ccttcccctc
     1201 tttacccaca gtgcaaatag ctgtacttcc ggccttaatt ccagcaagcc ctcctgcttt
     1261 agcatgcatt actcttctcc ctgtacattt tccaaatatt tatcaatttt tttttcaaaa
     1321 ttaatataat cgagcgtatc ataaagttca tcgcgcgttt gcatcttatt tagtacggat
     1381 ttttgggttc cttcgttgcg aatacatttt aacacttcta ttgcagcaaa attcattgcc
     1441 ctaaatacgc ttaaagggta taaagctacc cctaccccta cactttttaa ttgatctaaa
     1501 ttgaataaag gagtttggcc aaattctgta atgttagcaa gaacaggtat gcgcagcgtt
     1561 ttggtaaaag tttggtaaat cgccaaatca ttggctgctt cgagaaaaag catgtccgca
     1621 ccgatatctt gataataaca ggctcgttcc aacgtttttt ctacaccctc gacgctaagt
     1681 gcatctgttc tagctattac cataaaatct tcagtgtatc gcccatctaa tgcagccttt
     1741 aaacgactcc ccattatttc tttggatacc agtttttttc cttccctatg accacaacgt
     1801 ttagcagatt tttgatcttc aatatgggca ccacttgccc ctgccttgct taattcataa
     1861 aaggagcgat aaatatttaa agagcttcca aagccggtat ctgcatccac gactagaggt
     1921 aaggaaattc tactcgttat gcgtcggact tgtgataaaa catcttcgag tgatgtcatt
     1981 cccaaatctg gtatacccag atcggcgttc gcaatacctg cccccgataa atagagcgct
     2041 ttaaaaccaa gctgttctgc taataatgca caatacgcgt taattacgcc aataagttgt
     2101 aaaggctttt ctacggaaat tgcttcacgc aataagggaa taagtttttg agacaagcta
     2161 gctttctctc tctttctttg tcttattgaa tattgtaact ctgaacagaa ataagcaaaa
     2221 cattgcaaag caataaaaga acgcatgctg gtgcaagcta gagatttttg ggagcgattt
     2281 ttgatttata tacgtatttt ttttcttctt tttgatcgct tttattttta gtgttcatag
     2341 tttgcaaggg agtaaaattt ttagtgtgcc taacatcttt gacttggttc aattcttgca
     2401 ttcttggatg tgtaaagttt gcatttttat aatgaagtgc tttaaaaagt acttgtgttt
     2461 ctgttaaaag ctgttttttt ctgttttcca gatcaccctg catgcttagt aaagtatcaa
     2521 gttcatgtcg catggattgc gtagattgag gtattttttt tataacatcc tgtcgttcga
     2581 gtatattttc taataatttg tcggctcgta ttaacgcagg ctctagttct tgtt
//
LOCUS       DASTIB010000169         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1294498, whole genome shotgun sequence.
ACCESSION   DASTIB010000169 DASTIB010000000
VERSION     DASTIB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294498"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFP93_02870"
     CDS             <1..762
                     /locus_tag="VFP93_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843582.1"
                     /translation="LSLKRQGCYMKLKQRALFALYAYNREISLIFLKEKKNALKVLKE
                     YYQTVKALQEQYDDLFHHRQQIFDQAALDFLHFVRYESPLLQDVFKNADDDKVQEFIK
                     VYLAKRAKHQMLKENLAEQLDQQKKTLPENNPEFNVTQEQLNKERRYLKELILRAAKK
                     CSLKDDICVKMQLHRKEINQTIKGRFSPVIRHVMACDYELALNQTRQETMIREMKLMG
                     SYIPNYTSEIEETLSPLLKPNTLLKNISVKVARCA"
     gene            819..2018
                     /locus_tag="VFP93_02875"
     CDS             819..2018
                     /locus_tag="VFP93_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843583.1"
                     /translation="MFLQEYLENAIAEKNCKEVENLLLLFPSLAYENSAESYFKKLAQ
                     EKDVETIKVLWVGSDFGFDPLTYPGFEFLHQPEYESVKAIVNNNKKQRISETASLPIA
                     ESIRKWLASVLYVLTNIEPMYSTRTLKFASTNQTPQAISAPHLRFNLKSKEQAAALKR
                     LLWLFDIPCYGGMKNHFFLVTIPLTSFENIYRMLQGLTHFTRVDLNNFWQEGYYDEEY
                     TLLFNFCFNLREEMESIKMRAKNAILGMESHQIEHFIQTLTASVELKIKNTLQSRGIL
                     PLSACALSQEKIEFFSRLLNIPQFENNHLRGLQKTQKIMLLQVLFNKELQLPESKQTL
                     SKVEFKQFCDLYRKTLCEHLSFSYGTQESIAQIANTFTPGYYATQFAPLEEDSQPISK
                     KRKFSME"
     gene            complement(2046..>2341)
                     /locus_tag="VFP93_02880"
     CDS             complement(2046..>2341)
                     /locus_tag="VFP93_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843584.1"
                     /translation="GITQLDLSATEQLSWVEGNYVFAEGDFSMANGNQKIFGDVALSL
                     KAVLPENISGLGIPEATFNEYDVAMEQTTTIQFSDVVVEQAIQEASNAQSLEV"
BASE COUNT          810 a          422 c          417 g          692 t
ORIGIN      
        1 cttagtttaa agcgtcaagg ttgttacatg aaacttaagc aaagagcgct ttttgcgtta
       61 tatgcttaca atcgagaaat ttcattaatt tttctgaaag agaaaaaaaa tgcgctaaaa
      121 gttttaaaag aatattatca aactgttaaa gctttacaag aacaatacga tgatttattt
      181 caccatcgtc aacaaatctt cgatcaagca gccttagatt ttttacattt tgttcgttat
      241 gaatcgccgc ttcttcaaga cgtatttaaa aatgcagacg atgacaaagt gcaagaattt
      301 attaaagtat atcttgcaaa acgtgctaag catcagatgc ttaaagagaa tcttgcagaa
      361 caattggatc aacaaaagaa aacgttgccg gagaataatc ctgagtttaa tgttacccaa
      421 gaacaactta ataaagaacg acgttatcta aaagaactta tcctgcgcgc ggcaaagaaa
      481 tgcagcctga aggatgatat atgcgtaaaa atgcaattac atcgaaaaga aattaatcaa
      541 acaataaaag gccgtttttc accggttatt cgccacgtca tggcttgtga ttacgaatta
      601 gccctcaatc aaacacgtca agaaacgatg atacgcgaga tgaagttgat gggtagttat
      661 atcccaaact atacttcgga aatcgaagaa accttatctc ctcttttaaa accgaatacc
      721 ttactaaaaa atatttcggt aaaggttgcg agatgcgctt gaagatttgg tagtgctcag
      781 atacattggt ctgcatccgt ggagaagggg atctgatcat gtttttacaa gaatacttag
      841 agaatgcaat tgcagagaaa aactgcaaag aagtggaaaa ccttctctta ttatttccta
      901 gtcttgccta tgaaaattca gcggaatctt attttaaaaa attagcacaa gaaaaagatg
      961 tggaaacgat taaagtatta tgggtaggtt ctgattttgg ctttgatccg cttacctatc
     1021 caggttttga atttttgcat caacctgaat acgaatcggt aaaagccata gttaacaaca
     1081 acaaaaagca aagaatttca gaaactgcat cattaccgat cgccgaatct atcagaaaat
     1141 ggttagccag cgttttatat gtgttaacca atattgagcc tatgtattcg acccgtacgc
     1201 taaaatttgc atcaacaaat caaacaccac aagctatttc agcaccacat ttacgtttta
     1261 atttaaaaag taaagaacaa gcggcagcgt taaaacgatt actgtggctg ttcgatatac
     1321 cttgctacgg tggcatgaaa aatcactttt ttttggttac gataccatta acctccttcg
     1381 aaaatattta tcgcatgctg caagggttaa cccattttac gcgtgtagac ttaaataatt
     1441 tttggcagga gggttattat gatgaggagt ataccctttt atttaatttt tgttttaatt
     1501 taagagaaga aatggaaagc ataaaaatgc gggctaaaaa tgccatttta ggcatggaaa
     1561 gccatcaaat cgaacatttt atccaaacac ttacagcttc tgtcgaatta aaaattaaaa
     1621 atactctaca aagtagaggc atattgcctc tatctgcttg tgcattaagt caggaaaaaa
     1681 ttgaattttt ttcgcgtctt ttaaacattc cgcaatttga aaataatcat ttacgtggac
     1741 ttcaaaagac acaaaaaatt atgcttttgc aagtgctttt taataaagaa ttacaactac
     1801 ctgagtcaaa acaaaccctt tcaaaagtcg aatttaagca attttgtgat ctctatagga
     1861 aaacattgtg tgaacactta tccttttctt atggaacgca ggaatctatc gcgcaaattg
     1921 caaatacatt tacgcccgga tattacgcca cacagtttgc acctctcgag gaagattccc
     1981 aaccgatatc caaaaagaga aaatttagca tggaataata ctactcaaaa atttaagatt
     2041 gttgcttata cttcaaggga ttgagcgttg ctagcttctt gaatagcctg ttctacgact
     2101 acatcggaaa attgaattgt tgtagtttgt tccattgcaa cgtcgtattc gttgaaggta
     2161 gcttctggaa tacctaagcc agagatattt tccggtaaaa ccgctttaag ggacagagcg
     2221 acatcgccga agattttttg gttaccgttg gccatggaga aatcaccttc agcaaacaca
     2281 taattacctt caacccacga taattgctcg gtcgcagata gatcaagttg cgtaatacct
     2341 g
//
LOCUS       DASTIB010000170         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_376, whole genome shotgun sequence.
ACCESSION   DASTIB010000170 DASTIB010000000
VERSION     DASTIB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFP93_02885"
     CDS             <1..356
                     /locus_tag="VFP93_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9843585.1"
                     /translation="LNKLATSQNKTLPLLSKDVEIALQNYTFPGNIRELENILERAFA
                     LCDGKVITLSDLQLPVSAGIRLSGGLEHYLDDVERQAIIQALEQTAGNKSQAARKLGI
                     TFRALRYRLKKYDLE"
     gene            353..823
                     /locus_tag="VFP93_02890"
     CDS             353..823
                     /locus_tag="VFP93_02890"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET9843586.1"
                     /translation="MKGFHTLELVMVLAITSMVILLGISGLESFLTKNALEQGYALFH
                     DLHYARQAAIHQNTPVYICVSNDLQTCSLEKASGYILYTLKNTKKEIMASNYFPKLLL
                     LLKAGFGNEVIYFSPTGLCNVSSSFYVTSNKSSQSMRIVILASGRIRLEKNLMA"
     gene            857..1381
                     /locus_tag="VFP93_02895"
     CDS             857..1381
                     /locus_tag="VFP93_02895"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET9843587.1"
                     /translation="MKQKNLGFTLIEMMIAIVMLVIVMTVAIPSMQSTIQNNRISGLT
                     NELSSSLNLARSEAIKRGVSVSICAANASFNGCSNNWGTGWLIFVNPDENATFANNAS
                     EVLLRVHQVTGQGVTITTTPNVQVATYNSAGFAAVGSNVNFRIRASGCTGNNARNIAI
                     SLTGRLSTTQVACP"
     gene            1479..2093
                     /gene="pilV"
                     /locus_tag="VFP93_02900"
     CDS             1479..2093
                     /gene="pilV"
                     /locus_tag="VFP93_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HET9843588.1"
                     /translation="MLSKKSLSISSVKGFTLLEVLIAVVVFSVGLLGVAGLQILSLKL
                     SHDSLLRTTATLLAKDMADRMRANTSQIELGIYSAYNNPNGISQGNPSCVSKDSNGNS
                     VESSCAPQAMALHDFYEWNANVQGQASTAWHPEIKSFLPNAHGIVCIDSTPDDGLPPP
                     NNPACDNITSAGKQMFAIKIWWSERKDSLDDPTKLHQFVMNVEL"
     gene            2090..2875
                     /locus_tag="VFP93_02905"
     CDS             2090..2875
                     /locus_tag="VFP93_02905"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HET9843589.1"
                     /translation="MKLSPKQNGLSLVEILIGLALSMILLAGVLQVFLNSKQAYRLGA
                     AFSELQENGRFISDYIPRIVRNTGYRSTPLNTNFASLEEIFPEDKPSIQVLDNSGVNG
                     SDILTIRFQGSGDSLGNPDGTMRDCLNQPVDADEIVTNVFSISNLYELDCQSINPNAP
                     STVASNTLVSGVENLQVLLGEDLDGDDAPDRYVPPNFAGVNLNNVVSMRISILVRSTE
                     EVHKMLDTETYNLLGTLFVPTADYRIRRIFSFTIVLRNMISQV"
     gene            2878..>3217
                     /locus_tag="VFP93_02910"
     CDS             2878..>3217
                     /locus_tag="VFP93_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HET9843590.1"
                     /translation="MQKYGQQQGAVLITGMIILVVMVILGVSAVTFTALSEKMAGNQK
                     DKEIALQAAESALTEAENWLRQQITTPLAVNTCQTAPCDVWVENFGQWQSKPHSWWIG
                     VARMFNGILEG"
BASE COUNT          952 a          617 c          677 g          971 t
ORIGIN      
        1 tcttaaataa attggccaca tcgcaaaata aaaccttacc tttattaagc aaagatgtag
       61 aaattgcttt gcaaaattat acatttccag gcaacattcg tgaattagaa aatatcttag
      121 aacgtgcctt tgccttgtgt gatggaaaag tcattacact ttcggatctc caattacccg
      181 taagtgcagg gatccgactc agtggaggtc ttgagcatta cttagatgat gtagaacgtc
      241 aagcgattat acaagcctta gaacaaacgg ctggcaataa aagccaagca gcgcgcaaat
      301 tgggaattac ctttcgtgct ctccgatatc gcctaaaaaa atacgatctc gaatgaaagg
      361 atttcatacc ctagagttag tcatggtttt agccattacg tctatggtaa ttctattagg
      421 catttccggt ctggaaagtt ttttgacaaa aaatgcttta gagcaaggtt atgcgctctt
      481 tcatgatctg cattatgcgc gccaagctgc catacatcaa aatactcccg tttatatttg
      541 tgtctctaac gacttacaaa cctgtagcct agagaaagct tcaggctata ttttgtatac
      601 tctcaaaaat acgaaaaagg aaattatggc aagtaattat tttcctaaac ttttactctt
      661 gcttaaagcg gggtttggta atgaggtaat ttatttttca cccaccggtt tatgtaatgt
      721 ttcttcttct ttttacgtta catcgaataa atcttcgcaa tcaatgcgca tagtcatttt
      781 ggcaagtggt cgcattaggc tggaaaagaa tctaatggct tgatttatta ggccctttaa
      841 tctaattcat tagtatatga aacaaaaaaa tcttggcttc acacttattg aaatgatgat
      901 tgccattgtc atgcttgtga ttgtaatgac agtggccatt ccttcgatgc aaagtactat
      961 ccaaaataac cgtatttcag gtcttaccaa tgaattgtcg tcttcgctga atttggcgag
     1021 atccgaagcg ataaaacgcg gagtttccgt ttccatttgt gcggcgaatg ccagttttaa
     1081 tgggtgtagt aacaattggg gtacaggatg gttgattttt gtaaaccccg atgagaatgc
     1141 tacctttgca aataatgcat ccgaagtatt attgcgcgta caccaagtta ccggtcaagg
     1201 cgttaccata acaaccacac ccaatgttca agtcgcaacc tacaatagtg caggatttgc
     1261 cgcggtaggc agtaatgtta attttcgcat tcgagcgtcg ggttgtactg ggaataacgc
     1321 gaggaatatc gccataagtt taacagggcg tctttctacc acacaagttg cttgtcccta
     1381 accttttatc ggtttatttt tctctcttat cgccctaaat ctcttaaggt tcccattgta
     1441 actataacta tagtattcgc ttaactttgc gcaattttat gttgtcaaaa aagagcctta
     1501 gtataagcag tgtaaagggc tttaccttgc ttgaagtttt gattgcagtg gtagtgtttt
     1561 cagtaggtct tttgggggtg gcgggattac aaatattaag tttgaaatta agtcatgact
     1621 ctttgttacg gactacagcg acccttttag ctaaagatat ggccgatcgc atgcgtgcca
     1681 atacctctca aatagaactt ggaatttata gtgcctacaa taatcccaat ggtataagtc
     1741 aaggtaaccc cagttgcgtt tcaaaagata gtaacggaaa cagtgtagaa agttcttgcg
     1801 ccccgcaagc aatggcgctc catgattttt acgagtggaa tgctaatgta caaggtcaag
     1861 cttcaaccgc ttggcatcct gagattaaaa gttttctacc caatgcgcac ggtattgtat
     1921 gtattgattc aacgcctgat gatggtttac caccacccaa taacccagca tgtgacaaca
     1981 taaccagcgc aggcaaacag atgtttgcta taaaaatttg gtggtcagag cgaaaggata
     2041 gtttagatga tcccacgaaa ttgcatcaat ttgtgatgaa tgtagagcta tgaaattatc
     2101 ccctaaacaa aacggcctat ctctcgtaga aatattgatt ggacttgctt taagtatgat
     2161 tctgttagca ggcgttttac aagtattttt aaatagcaaa caagcctata gactgggggc
     2221 agccttcagc gaactgcaag aaaatggccg ttttatatca gattatattc cacgtattgt
     2281 ccgcaatact ggttatcgtt ccacgccttt aaatacaaac tttgcatctc ttgaagaaat
     2341 ttttcccgag gataaaccct cgatacaagt gttggacaat agtggcgtaa atggtagtga
     2401 tattttgact atccgctttc aaggtagtgg tgatagtcta ggaaacccgg atggtaccat
     2461 gcgcgattgt ttaaaccaac cggtggatgc agatgaaatc gtcaccaatg tcttttctat
     2521 tagtaatttg tatgagctgg attgtcaatc tatcaatccg aatgcgccat cgaccgttgc
     2581 aagcaacact ttggtttcag gtgtggaaaa tttgcaagta ctcttagggg aagatctcga
     2641 tggcgatgat gcacctgatc ggtatgttcc tcctaatttc gccggggtca atttaaataa
     2701 tgtggtaagt atgcgcatca gtattttggt acgcagtacc gaagaagtgc acaaaatgct
     2761 tgataccgaa acgtataacc tactaggaac tttatttgtt cccacagcgg attaccgaat
     2821 acgacgaatt ttttctttta ccattgtact gaggaatatg atttcgcagg tttaaaaatg
     2881 caaaaatatg ggcaacaaca aggtgcggtt ttaattacgg gcatgattat tctcgtggtt
     2941 atggtaattt tgggtgtgag cgccgtcaca tttaccgcgt taagtgaaaa aatggcaggg
     3001 aatcaaaaag ataaagaaat tgctttgcaa gctgcagaat cggccttaac agaagcagaa
     3061 aattggttac gacaacaaat tactaccccc cttgccgtaa acacgtgtca aacagctccg
     3121 tgtgatgttt gggtagaaaa tttcggacaa tggcaatcaa aaccgcacag ttggtggata
     3181 ggggtggctc ggatgtttaa tggaatatta gaaggcg
//
LOCUS       DASTIB010000171         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1478719, whole genome shotgun sequence.
ACCESSION   DASTIB010000171 DASTIB010000000
VERSION     DASTIB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478719"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFP93_02915"
     CDS             <1..559
                     /locus_tag="VFP93_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9843591.1"
                     /translation="PWVAGIIVIGGICAVATWIIGPTKGLFAAADDGLIPSKLGKVNR
                     FGAPLPILLLQGAICSMLCLVYLLLPSVKSAYWFLSAMAAQLAFFMYAILFISAIRLR
                     YVSPQQRAFKIPGGNIGMWLLAGMGFVVSLFVIALGFIPPSDVDVGSVWWYETLLIGG
                     DLSILLPVFFLGRKHKLQIDAFQPA"
     gene            663..1328
                     /locus_tag="VFP93_02920"
     CDS             663..1328
                     /locus_tag="VFP93_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HET9843592.1"
                     /translation="MLNNFLFLISTLLVVLFGVFTLKLGKNALIAWIALLGVLANLFV
                     LKQIQLFGLNATASDVYAVGSLLGLNILREQYDLKTCRHAILVSFVCLIFFCLMGQVH
                     LLYVPSAYDSTQLAYQTLLDPNVRIIVASITVFFSVQLFDVYFYSLVLKRFMKALWMR
                     NTISLIVSQLIDTILFSFLGLYGIVDNIGEIIVVSYCIKLLAIFCMVPATSFSKSWFK
                     AVT"
     gene            1325..>2128
                     /locus_tag="VFP93_02925"
     CDS             1325..>2128
                     /locus_tag="VFP93_02925"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HET9843593.1"
                     /translation="MNKYFSFELIHQSKKSRARVGRIYTPHGVIDTPNFVAVGTNGSL
                     KALTNHDVSELGLQLMFCNTYHLMLQPGSETVAKAGGLHRFINRNLPIITDSGGFQVF
                     SLAYGGIADELKSKGNKKSDGSVLKITEEGVIFRSYRDGAKFTLTPESSVLAQKDFGA
                     DIIIPLDELPPYHIGKEALKNSFDRTHRWELRSLETHLKDRKQQAMYAVIHGGIDADL
                     RKLSCEILGAQAFDGFAIGGSLGKTKQELFETVEATVPHLPPETPNHLLG"
BASE COUNT          578 a          379 c          455 g          716 t
ORIGIN      
        1 gccctgggtt gccggcatta tcgttatagg cggcatatgc gcggttgcaa cgtggattat
       61 aggacctacc aaagggttat ttgccgcagc cgatgatgga ttaattccct ccaaattggg
      121 taaagtaaat cgctttggcg cacccttacc catactttta ttgcaaggag ctatttgctc
      181 catgctttgc ttagtatatt tattactacc ttcggtcaaa agtgcttatt ggtttttgtc
      241 cgctatggcg gcacaattgg ctttttttat gtacgccatc ttatttataa gcgcgattcg
      301 gttacggtat gtaagcccac aacagcgtgc gtttaagatc cccggtggca atataggcat
      361 gtggctactc gcaggcatgg ggtttgtcgt gagtttattc gtcattgctt taggatttat
      421 tccacctagt gacgtagatg taggtagtgt ttggtggtat gaaacgctcc ttattggggg
      481 tgatttgagt attctactgc cggtattttt tttaggtcga aaacataaac tccaaataga
      541 tgcgtttcaa cctgcttaat ggttgctgaa atttgtaatt aaacttaata atctatagca
      601 atctttatta attcaaaaag atacgctaaa ctgagccagt ttacacaatg ggaaacattc
      661 ctttgctaaa taattttctt ttcttaatta gtaccttgtt ggtagtgttg ttcggcgttt
      721 ttacgcttaa actgggaaaa aatgcactta ttgcctggat tgccttatta ggcgttttgg
      781 caaatttatt cgtattaaaa caaattcaat tatttggatt aaacgccacc gcaagcgatg
      841 tgtatgccgt aggaagttta ttaggtctta atattttgcg tgagcagtac gatttaaaaa
      901 cctgtcgtca tgcaattttg gtttcgtttg tgtgtttaat ttttttctgt ttaatggggc
      961 aagttcatct actctatgtt ccaagtgcgt atgattctac gcaattagcc taccaaacgc
     1021 ttttagatcc caacgtgcgc attattgtgg cctccattac agttttcttc agcgtacaat
     1081 tgtttgatgt gtatttttat agtttagttt taaaacgttt tatgaaggca ctatggatgc
     1141 gcaatactat atcgttaata gtatcgcagt tgatcgatac tattttattt agctttttag
     1201 gactttatgg aatagtcgac aatattggcg aaattatcgt agtaagttat tgcataaaac
     1261 ttttagctat tttttgcatg gtgcccgcta caagcttttc taaatcgtgg tttaaagcag
     1321 taacatgaac aagtattttt cctttgaatt aatacatcaa tctaaaaaat cgcgcgcacg
     1381 tgtgggtcgc atttatacgc cacacggtgt aattgataca ccgaattttg tcgcggtagg
     1441 aactaatggc agtttaaaag cattaaccaa tcacgatgta agtgaattag ggctacagtt
     1501 aatgttttgt aacacttacc acttaatgtt gcagccgggt tctgaaacgg tggcaaaagc
     1561 tggagggctt catcgtttta tcaatagaaa tctgccgatc attacggatt cgggcgggtt
     1621 tcaagttttt agccttgcct atggtggtat tgcagatgag ctaaaaagta aaggtaataa
     1681 aaaaagcgat ggtagcgttt taaaaattac cgaagaaggg gtaatttttc gatcgtatcg
     1741 ggatggcgct aaatttacct taacacctga gtcttctgta ttagcgcaaa aagactttgg
     1801 cgcagatatt atcattcctc tcgatgaatt gccgccttac catattggca aagaagcttt
     1861 aaaaaattca ttcgatcgaa cccatcgctg ggaattgcgg tctttagaga ctcatttaaa
     1921 agatcgtaag caacaagcga tgtatgctgt gatccatggt ggcatcgacg cagatttgcg
     1981 taaattaagt tgcgaaattt taggcgcaca agcctttgat ggttttgcca ttggtggcag
     2041 tcttggtaag actaagcaag aattgttcga aactgtagaa gcgaccgtac cacatttacc
     2101 gcctgaaact ccaaatcatt tattagga
//
LOCUS       DASTIB010000172         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1029366, whole genome shotgun sequence.
ACCESSION   DASTIB010000172 DASTIB010000000
VERSION     DASTIB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029366"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFP93_02930"
     CDS             <1..374
                     /locus_tag="VFP93_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HET9843594.1"
                     /translation="SFELRKLTETKAGNYEGIKATITISKNSVFLKNLYPEKRMYFPR
                     EMVLSEAAIYPSLINELYIALGEPLESGWSMRIYIKPYVRLIWLGGLLMALGGMGALI
                     KNKTMPIRKRASKTLWLKKMA"
     gene            353..886
                     /locus_tag="VFP93_02935"
     CDS             353..886
                     /locus_tag="VFP93_02935"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET9843595.1"
                     /translation="MAKKNGLALIAIGTFCLLVVLFVRALSINPHALPLERKGALMPT
                     FALSNLWQEHTLVTQQDLLNKVVLLHFWASWCGVCHKEHETLLTLKDTGVYIVGVSYR
                     DDPKAAKKWLSTKGDPFTINISDVNGQLGMELGVYGTPETYVIDKNGHLQHRFVGPLT
                     LSSYRKEIEPWINALKQ"
     gene            862..1248
                     /locus_tag="VFP93_02940"
     CDS             862..1248
                     /locus_tag="VFP93_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET9843596.1"
                     /translation="MDKCFKAVIIFFVIVFSSISLLNAQTMTEKQQQVYDKLLFELRC
                     VTCANQNLAESQAPAALSMKYAVAQWVLEGKTEAQIRHALTARYGDYISYRPPLKSST
                     YFLWWAPIFFSLFAVLAFYRIVRRKC"
     gene            1242..2270
                     /locus_tag="VFP93_02945"
     CDS             1242..2270
                     /locus_tag="VFP93_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843597.1"
                     /translation="MLIFCSCLALIFCFVMLLLWIQLPAFAHKKIVLLVFFVFLSITS
                     SLLYGKIGASFDLQAFYAIHKIYDTLKDLQASKTLTAPLVDAHLQQISKQLPAHAGAW
                     EHLGDVYQNLQWYSRAEKCYLQSYNLNNKNLAVQIKLIYASSLASGGRLEGHNLMRVK
                     TLLQNYPNKVELLNLVATDHFQRGKYAQALQQWEAMLKYVSNEEEQKPILLAITKVKS
                     KLGKLNINNSDIRLEILVKVSKNFKRKIPPETPVFVFAKLAEEKAVPLAVQKLTFKDL
                     PKKIVLTTLDAMLPDNRLRVHQKVIVGARIALSNNALGLKGDLETFSDVVEVKNHHQL
                     ELILDEEG"
     gene            2411..3139
                     /locus_tag="VFP93_02950"
     CDS             2411..3139
                     /locus_tag="VFP93_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843598.1"
                     /translation="MIRSNYSDESLTLMAYLAENAHAHANTTYVVYIETGWEAHNWLT
                     KVEQGEVFAQRLGFTPIRLKSKASFAQLVEDRKRFPSKKFQWCAGFLKGLPLLEWLDL
                     KDPDLQWIIALPKRQAMYRAPIVESITECPYHGERTVLHPIRTLTNSQKDTLLQKYGL
                     IPTYQRSKECDPCVHSSAQDLLNMTQSDIDKTIQLERVIDATMYTSGLDKLIREIAMQ
                     HKSARQYTMDSFSMGCGDPFGCGL"
     gene            3188..>4903
                     /gene="prsT"
                     /locus_tag="VFP93_02955"
     CDS             3188..>4903
                     /gene="prsT"
                     /locus_tag="VFP93_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HET9843599.1"
                     /translation="MKTQRIVRILLLMVGFLQGCNQSETSETNLQEASKSFEEQKYQE
                     SLIYLKKALKSKPSDPEIRKRLGEVWLKLGDPNACIEHLKKAQDLRMPDNEVTLSLAQ
                     CYFYKKDFEQLDALDFSENMLEVSNRDYAILSAYKAQAALVKNESSKAYDHLQRAELL
                     MAEEPMVLLSKAQWLIYEKQFQEALHLLDKVTAQSPNNAQAWGLKGDLYRFQQKTSKA
                     EEAYTKALQNPVNKLDQINYFLYRSLVRIYLNKLDQAQEDIVKLQKLKAQHHGLHYVN
                     GLLALAKKDFPQAIQNFQKVLEQDENFIYTHYFIGLTHVLTKNYHSARDHLIRFTRAR
                     PSHVLGQKMLGFVELQLQDYNHAKSRLEKVKELNQKDSDVYHMLGQIYIKEGDIEQGA
                     NYLSKAQTLEPKNAEMTLKLGIAQMDLGNTYAASGAFDQVLSQIPNHKDTLLLKFINE
                     FKAKHYPAAMELAKTYTQTHPKDPLGFNLQGMVYVAKNQTQQADDAFHAALKLAPHDP
                     NAHTHLAMLSLKKRDVTQAIAHYKKILERHQHTLSMLKLAKIYLKETRDESEAVKLLE
                     LAIKSQ"
BASE COUNT         1657 a          852 c          977 g         1417 t
ORIGIN      
        1 atagctttga attgcgtaaa ctcacagaga caaaagcagg caattatgaa ggtataaaag
       61 caaccattac aatttctaaa aatagtgtat ttttgaaaaa tttgtaccca gaaaaacgta
      121 tgtactttcc aagagaaatg gttttatccg aggcagccat ctatcctagt ttaataaatg
      181 agctttatat agctttgggt gaacccttag aatcgggatg gtcgatgcga atctacatta
      241 agccttatgt gcgtttaata tggttaggcg gattattaat ggcattggga gggatgggtg
      301 cacttattaa aaataaaacc atgcctataa ggaaaagagc gagtaaaaca ctatggctaa
      361 aaaaaatggc ttagcactta tcgcaattgg tacattttgt ttactggttg ttctatttgt
      421 ccgggcttta tctataaatc cgcatgcttt acctttagag cgcaagggtg cgctcatgcc
      481 tacctttgct ttatctaatt tatggcaaga gcatacgcta gtgacgcagc aagatttatt
      541 gaataaagtg gttttattac acttttgggc cagttggtgt ggtgtatgtc acaaagaaca
      601 cgaaacgtta ttaacgctca aagatacagg cgtgtacatt gtgggtgtga gctatcggga
      661 tgatcccaag gctgcaaaaa aatggctttc tactaagggg gatccattta ccattaacat
      721 cagcgatgta aatggccagt taggtatgga gttgggtgtg tatggtacgc cagaaaccta
      781 cgtgatcgat aaaaatggcc acttgcagca tcgttttgta ggacctttaa ccttatcaag
      841 ttatcgtaaa gaaattgagc catggataaa tgctttaaag cagtaattat tttttttgtc
      901 atagttttta gcagtatttc gttattgaat gcgcaaacta tgacggaaaa acaacaacaa
      961 gtatatgaca aattgctctt cgaattacgt tgtgtaacat gcgctaatca aaatttggcg
     1021 gaatcacagg cgccagcagc tcttagtatg aaatatgcag tagcgcaatg ggtgcttgag
     1081 gggaaaaccg aagcacaaat acgccatgca ctcaccgcgc gctatgggga ttatattagt
     1141 tatcgtcccc ccttaaaatc aagcacgtat tttttatggt gggcaccgat ctttttttcg
     1201 ctatttgcag tgttggcttt ttatcgcatc gtaagaagaa aatgttaata ttttgtagtt
     1261 gtttggcatt aattttttgt tttgtaatgc ttctgctttg gatccaatta cctgcatttg
     1321 cgcacaaaaa aatagtgctg ttggtttttt ttgttttttt aagtataacg agcagcttgc
     1381 tgtatgggaa aattggcgca tccttcgact tacaagcctt ttacgccatc cataaaattt
     1441 acgatacgtt aaaagatctg caagcaagta aaacacttac agcacccctg gtagatgctc
     1501 atttacaaca aatttcaaaa caattaccag cccatgcggg tgcttgggag catttgggag
     1561 atgtatacca gaatttgcaa tggtattcgc gtgccgaaaa atgctattta caatcctaca
     1621 atctaaataa taaaaattta gcggtacaaa taaaacttat ctatgcctca agtctcgcat
     1681 ccgggggtag actagaaggg cataacttaa tgcgtgtaaa aaccttgttg caaaactacc
     1741 ctaacaaagt tgagttgcta aatcttgtag caaccgatca ttttcaaaga ggcaagtacg
     1801 cgcaagcgct acaacaatgg gaagctatgt taaagtatgt ttccaacgag gaagaacaaa
     1861 agcctatttt attagcaatt actaaggtaa aatcgaagtt aggtaaatta aatataaaca
     1921 attctgatat tcggttagaa attttggtta aagtgtctaa aaacttcaaa cgcaaaatac
     1981 cacctgaaac tccggtgttt gtttttgcaa aattagcaga ggaaaaggca gtacctttag
     2041 ctgtgcaaaa attgactttt aaggatttac ctaaaaaaat cgttttaaca acgctcgatg
     2101 ctatgctccc agacaatagg ttgcgtgttc atcaaaaagt gatagtagga gctcggattg
     2161 ctttatcgaa taatgcattg ggcctaaaag gcgatctaga aacttttagt gatgttgtcg
     2221 aagttaaaaa ccatcaccaa ttggaattaa tacttgatga ggaaggttag aatatggagt
     2281 taactatggc taaaaaaatc tataaagatg cggatgaaaa atttgatgtc ttcgtgatat
     2341 taatagtcat cgcagcaatc gttacaggtg tagtgtattg gttagcggga atgcctgctt
     2401 aatgcatctt gtgattcgca gtaattatag cgatgagtct ttaacactca tggcctattt
     2461 agctgaaaat gctcatgcac atgctaacac tacttatgtt gtgtatattg aaacgggatg
     2521 ggaagcacat aattggttaa ccaaggtcga gcaaggtgaa gtattcgcac aacgtttagg
     2581 ctttacgccc attcgactaa aatcgaaggc aagttttgcc caattggtgg aagatcgcaa
     2641 acgatttcct agcaaaaaat ttcaatggtg cgcgggtttt ttgaaaggat tacctttatt
     2701 agagtggtta gatctcaagg atccagattt acaatggatc attgctttgc caaaacgaca
     2761 agcgatgtat cgggctccta ttgtggaatc tatcacagaa tgcccttacc atggtgaaag
     2821 aacggtgtta catcccatac gtactttaac caatagtcaa aaagatactt tgttacaaaa
     2881 atatgggtta atacctacgt atcaaagaag taaggagtgt gatccgtgtg tgcacagttc
     2941 tgcccaagat ttacttaata tgacacaatc cgatattgat aaaacgatac aactggaacg
     3001 cgtcatagac gctacgatgt atacatccgg tcttgataag ctaatccgag aaattgcaat
     3061 gcagcataaa tctgcacgac agtatactat ggatagtttt tcgatgggtt gcggagatcc
     3121 atttggttgt ggtttgtaaa atatctttat acgatacgtg tgtggtttag ttattaatgg
     3181 aaggtttatg aaaacacaaa ggatagtgcg tattttgcta ttgatggttg gatttttgca
     3241 aggttgcaac caatcggaaa catcagaaac caacttgcaa gaggcttcca aatccttcga
     3301 agagcaaaaa taccaagaaa gtttaattta tttaaaaaaa gcattaaaaa gtaaaccatc
     3361 cgatcccgaa attcgaaaac gtttaggtga agtttggtta aaactggggg atccgaatgc
     3421 ctgtatcgaa cacttaaaaa aggcgcaaga cttacgcatg cctgacaatg aagtaacctt
     3481 gtctttagca caatgttatt tttacaaaaa agattttgaa caactcgatg cgcttgattt
     3541 ttccgaaaac atgttagaag tttccaatag agattatgca attttatcgg cgtataaagc
     3601 tcaagcagca ttagtaaaaa atgaatcttc aaaagcctat gatcatcttc aacgcgcaga
     3661 attacttatg gcagaagaac ctatggttct tttaagtaaa gcgcaatggc tcatctatga
     3721 aaaacaattt caagaggctt tgcacctatt agacaaagta accgcgcaat ctcctaacaa
     3781 tgcccaagct tggggattaa aaggggatct ataccgtttt cagcaaaaaa caagcaaagc
     3841 agaagaagcg tataccaaag cgttgcaaaa ccctgtaaat aaattagatc aaataaatta
     3901 ttttttatat cgttctttag tgcgtattta tttaaataaa ctggatcaag cacaagaaga
     3961 tattgtgaaa ttgcaaaaac tgaaagccca acatcacgga ttgcattatg taaacggttt
     4021 attagcactg gcaaaaaaag attttccgca agcaatccaa aattttcaaa aagtcttaga
     4081 acaagatgaa aattttattt acacccatta ttttattggt ttgacgcatg ttttaacaaa
     4141 gaattatcat tctgctcggg atcatttaat tcgctttacg cgtgcaagac cttcccatgt
     4201 attgggacaa aaaatgcttg ggtttgttga attacaatta caagattaca accacgccaa
     4261 aagccgcttg gaaaaagtaa aagaattaaa ccaaaaagat tctgatgtgt accatatgct
     4321 aggacaaata tatataaaag aaggggatat agaacaaggg gcaaattact taagtaaagc
     4381 acaaactttg gagccgaaaa atgcagaaat gacactgaaa ctcggcatag cgcaaatgga
     4441 tttaggaaat acttatgctg catcgggggc gtttgatcaa gttttatctc aaattcccaa
     4501 tcataaagac actttattat taaaatttat taatgaattt aaagcaaaac attacccagc
     4561 tgcaatggaa ctagcaaaaa cctatacaca aacgcacccg aaagatccgt taggttttaa
     4621 tctccaagga atggtgtatg tggcaaaaaa tcaaacgcaa caagcggatg atgcttttca
     4681 tgcagcctta aaattggcac cacatgatcc caacgcgcat acgcatttag cgatgttatc
     4741 gcttaaaaaa cgcgatgtaa cacaagcaat agcgcattat aaaaaaatat tagagcgtca
     4801 tcaacacacg ctttctatgt taaaactagc gaaaatttat ctcaaagaaa cccgagatga
     4861 atcggaagct gtaaaacttt tggaattggc catcaaaagt caa
//
LOCUS       DASTIB010000173         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_434576, whole genome shotgun sequence.
ACCESSION   DASTIB010000173 DASTIB010000000
VERSION     DASTIB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434576"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..2097)
                     /gene="recG"
                     /locus_tag="VFP93_02960"
     CDS             complement(16..2097)
                     /gene="recG"
                     /locus_tag="VFP93_02960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET9843600.1"
                     /translation="MLKTLKSKASSSYNWDTSLSELSGVGPSFASKLKKIQLETMRDA
                     IFHFPLRYEDRTHLLPLRDVKPLPKALIQGKILKTQQHRGKRTWLELVIQEFEQTFSL
                     VLFHFTAKQQIIFERKPYVRCFGEIKFYKNTLVMVHPEIQFLTQLQQPLEETLTPIYP
                     STQGLHQGYLRKIIHQAVDWAVENVQDVLPDVFLTIGKKIPFARAIDSIHRPKPHEAH
                     LIQEKEHPALQWLCVEEFLAHQLNLKKARSLQNQEMAQPFFDQMTLIASFLKNLPFKL
                     TLAQQRVWTEIKDDLAKNQPMMRLVQGDVGCGTTVIAALASLQVLQNGICVALMAPTE
                     ILAEQHYNQFKQWFEPLHVDVVLMCAKTKNKSLCIQKSCFIAIGTHALFQDSVQFPSL
                     GLIIIDEQHRFGVQQRMQLLRKGATYLPHQLIMSATPIPRSLAMTKWADMDISIIDEL
                     PPHRTPITTLCVPQSKKTEIIKRVEEICRTRQQVYWVCPLIEESEKLAIQAVTLLFQE
                     LKQQLPHHNIGLVHGKMKTEEKEKMMRAVKDGSCQILVATTVIEVGVDVPNATYMIIE
                     NAERLGLAQLHQLRGRVGRGTQKSYCLLLFKSPLSFNAKKRLETIRKSQNGFEIAHQD
                     LQLRGTGELLGSKQTGLPTFKALDLGRHANLMETTKKCLDAHYTQLNIAILTDELARR
                     WLGENPLYIKV"
     gene            complement(2099..>2615)
                     /locus_tag="VFP93_02965"
     CDS             complement(2099..>2615)
                     /locus_tag="VFP93_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9843601.1"
                     /translation="AACILTHSQGDHPHMESVGPSKPLVIKGTEGLVVNYATCCYPIP
                     GDLIIGRLIAGKGVEIHTEQCAKVVNFRRNPLKCINVQWEEEVQGEFPVCLYVDVLNG
                     RGVLATLANAIAEANANIINVSVDERDGLHNTVKFIVEVRDRAHLARIMRRLRAVQSV
                     TRLIRQRFGTY"
BASE COUNT          724 a          537 c          513 g          841 t
ORIGIN      
        1 cacaaaaaag ttcttttaaa cttttatata aagaggattt tctcctaacc atctgcgtgc
       61 caactcatcc gtaagaattg caatatttag ttgcgtataa tgcgcatcta aacatttttt
      121 tgttgtttcc atgagatttg catgacgccc tagatcaagc gctttaaaag tcggcaatcc
      181 ggtttgcttg ctgcctaata attctcctgt gccacgcagt tgaagatctt ggtgtgcaat
      241 ttcaaagccg ttctgtgatt ttctaattgt ttctaatctt tttttcgcat taaaggacaa
      301 tggcgattta aataataaaa gacaataact tttttgcgta ccccttccaa cgcgcccgcg
      361 tagttgatgc aattgcgcta aacctaaacg ttcagcattt tctataatca tgtaggtggc
      421 gttgggaaca tcaactccaa cttcgattac cgttgtagcg acaagaattt gacagctgcc
      481 gtctttaaca gcgcgcatca ttttttcttt ttcttcagtt ttcattttgc catgaacaag
      541 accaatgttg tgatgaggca attgttgttt caactcttgg aacaacagtg ttaccgcttg
      601 aatggctaat ttttccgatt cttctattaa aggacatacc caatatactt gctggcgtgt
      661 tctacagatt tcttcaaccc gctttattat ttccgtcttt ttactttggg gaacacaaag
      721 ggttgtgatc ggtgtgcgat ggggtggcaa ttcatctatg atactgatgt ccatatctgc
      781 ccacttagtc atagctaacg atcgtgggat aggtgttgcc gacataatga gctggtgcgg
      841 taagtatgtg gctcctttgc gcaataattg catccgctgt tgtactccaa aacgatgctg
      901 ctcatcaata atgattaaac ccaaacttgg aaattgtaca ctgtcttgaa acaacgcatg
      961 cgtgcctatc gcaataaagc aggatttttg tatgcaaaga gatttatttt ttgttttggc
     1021 acacattaaa accacatcca catgcaaagg ctcaaaccat tgcttaaatt gattataatg
     1081 ttgctccgct aatatttctg tgggtgccat taatgcaaca catattccat tttgtaaaac
     1141 ttgtaaactt gcgagagccg cgattacggt tgtgccacag ccaacatccc cctgtactaa
     1201 ccgcatcata ggctgatttt ttgctaaatc gtcttttatt tcagtccaca cacgttgttg
     1261 ggcaagtgtt agcttgaaag gtaaattttt taaaaatgat gcaatcaaag tcatttgatc
     1321 aaaaaagggt tgagccattt cctgattttg cagacttctc gctttcttta aatttaattg
     1381 atgtgccaaa aattcttcga cgcacaacca ttgcaacgct ggatgctctt tttcttgaat
     1441 taaatgtgct tcatgtggct tgggacgatg tatggaatcg atagcacgag caaaaggaat
     1501 tttttttcca attgttaaaa aaacgtctgg aagcacgtct tgcacgtttt caacggccca
     1561 atcgaccgcc tgatgaatga tttttcgcag ataaccttga tgtaatcctt gggtcgaggg
     1621 ataaataggg gttaaggttt cttctaaggg ttgctgcaat tgggttaaaa attgaatttc
     1681 cggatgcacc ataactaaag tatttttata gaatttaatt tccccaaaac agcgcacata
     1741 tggcttacgt tcaaaaataa tttgttgttt agcggtaaaa tgaaataaaa ccaaagaaaa
     1801 agtttgctca aattcttgta taactagttc gagccatgtt cttttgccgc gatgttgttg
     1861 ggttttaagg atttttcctt gaatgagcgc tttcggtaat ggttttacat cgcgcaatgg
     1921 cagtagatgg gttctatctt cgtaacgtaa tgggaagtga aaaattgcat cgcgcatagt
     1981 ttctaattga atttttttta gtttggatgc aaagcttggt cccacgccag agagctctga
     2041 aagcgaagtg tcccagttgt aggaagatga ggccttagat ttaagggttt tcagcacatt
     2101 aataggtacc aaatctttgt cgtattagac gggttacaga ttgcaccgca cgtagcctgc
     2161 gcataattct tgccaaatgt gcgcgatccc gcacttccac aataaacttc acggtattgt
     2221 gcaacccatc acgctcatcc acgcttacat taataatatt ggcattggct tccgctatag
     2281 cattagcaag ggttgctaac accccacgcc catttagcac atccacataa agacaaacag
     2341 gaaactctcc ttgaacttct tcttcccatt gtacattaat gcacttgaga ggattgcgtc
     2401 taaagttcac aacttttgcg cattgctcag tatgaatttc tacgcctttc ccagcaatta
     2461 atcgaccaat tatgagatcg cctggaatgg gatagcaaca agtagcataa tttacgacta
     2521 aaccttccgt gcccttaatg actaagggtt ttgaaggtcc taccgattcc atatgcggat
     2581 gatcaccttg gctgtgtgtt aaaatacaag cagca
//
LOCUS       DASTIB010000174         5752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1246452, whole genome shotgun sequence.
ACCESSION   DASTIB010000174 DASTIB010000000
VERSION     DASTIB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246452"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1391
                     /locus_tag="VFP93_02970"
     CDS             342..1391
                     /locus_tag="VFP93_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843602.1"
                     /translation="MQDLENGPLPKTLHTQTLELIAKVLLRNPESLAFTFTNANKTLS
                     VHFQDNSINITELLQKANLNHIQLDEADSELYLEIFNEDPNTTTYKDLTAAEKIVLRG
                     FTSTSYFINNLLYGNYKYILECGYLNNQKVQDILLQTLFVASGLNKIMPETLSFKTYR
                     GEGSTLDPEIEKRKVYVTDQEYYQERAFKSTTSSYEVAKRFSRNKVLIEFEHAYGKDI
                     SGISYFKNEKEYLLNAEKIQWLSHKQEKELNIFKARVVNPLDPIRDAVTDREIDQFLK
                     LKEYAEYLDIEFDFLTDFNRAQLPPQPSWLDDLLSPINTCTALWDSTKKFVYDTFAQE
                     YDTYSEEEDLSFSIF"
     gene            complement(1979..4114)
                     /locus_tag="VFP93_02975"
     CDS             complement(1979..4114)
                     /locus_tag="VFP93_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HET9843603.1"
                     /translation="MRALEFKKSLTFAFLTSSIFFSNAIHAQELITGAENCSDPGSVC
                     TTEGVLFTGDFLLTVNNGDQIGNVGGVSAETINANQGQIAFQGTSIVTGSIGTSLLPI
                     NGIGIQGAGKTVTFVGDVYTKNGIGFDNAVSGSAIVQPGVNLNTEVFTLNPGTGIVTY
                     QGNSTTAGNLGDLGGSLEAVNVNAGTLNLGHGIFATDTTVNAGAALNTIASNTITGNL
                     IIDGTVNLNTNTLTVTGDAPISATGNLQTTLTNAPSNGKLAIGGTATCTNCTVTVNVS
                     GDSIATGKQFTVVDGGVYAGDLPVIIGNNATYNFVPSTDNTDLFLTVTRTPFTDTATD
                     PNLAGVAGFLEGIAFDTLLNNPEFAPLIQSLSTLDAAQFQQALSTLIPISDGSIMTLS
                     HLAVDEVLLNISQRLATMSPMFASMQAGNYAAGDWDTGAGNWIQIFGNDVNHDARSGF
                     AGFDAWNAGFAIGFDKEWWRNVLIGIGFSYAYTDVDSKAAAGNNLDIQTYNGFIYGNW
                     RITPPIYWDAVLSVAYHDYDTNRYIVSPGVPPINVRAEGEFGAWQGTFFTELGYELLI
                     DHWFITPHINFKYSHLDSKRLEETGAGALNLSVEYDDVNEVVLGGGLRLGYIMEMDYN
                     VQLMPHLRAQVFHDFENDAQISTANFVGLGGAFVTPGLSPDDTTFRVGAGLDVACPHN
                     WYFTINYDYKFRDDFDAHGGWVKFRWEWV"
     gene            4348..5136
                     /locus_tag="VFP93_02980"
     CDS             4348..5136
                     /locus_tag="VFP93_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843604.1"
                     /translation="MFHSKNSKNHWITKYLDTLHLKNIIASTAKRASAFGSEQIVIRS
                     FFPSPLAHAVQYIFTNLSGSLSKELVLYCLNNKEHTDSIECLVIPIPEEDLKALDKAK
                     KTQDWKTFFARIKHVGQNSVRVGLHNIVTLSYADFATNAGATMGSLLAQGVFFGMVGP
                     PSLLTAPLYFLTEGSIKSLSAFLGNQLCRQNTLHAEDPIFLDALTTVLQKMHIQQEEQ
                     EDYECQTIDGAEQSDLLQSWELFKTPIPKVQQNEQFAVIDDYKP"
     gene            complement(5139..5321)
                     /locus_tag="VFP93_02985"
     CDS             complement(5139..5321)
                     /locus_tag="VFP93_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET9843605.1"
                     /translation="MDRKLLDILACPVCKGPLVYKKQEKELICKLDRLAFPIREDIPV
                     MLEEEARQLPADEEVE"
     gene            complement(5302..>5752)
                     /locus_tag="VFP93_02990"
     CDS             complement(5302..>5752)
                     /locus_tag="VFP93_02990"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET9843606.1"
                     /translation="PLREPKSRLKQVDYILSDNVKIIEGFTIQWEQQGVYRLQDTSEQ
                     LLLSHFKGMKVHAVTGIACPNRFFRMLQSHGLLPLEHSFPDHHFFQSKDLYFQDNAPI
                     LITEKDAVKCRGLKIRSPIWVVRLEVTLEEAFISRILEDVENGQKVA"
BASE COUNT         1838 a         1144 c         1018 g         1752 t
ORIGIN      
        1 caacacagca tctcctgcaa cactaacacc catcgttata agagaggtaa gttggtaatt
       61 ggtgatcccg cctgcgatgt agctggtttc tactgtgtta agcacgttca tatatctctc
      121 ctttacttct aattttcgaa ccaatattaa gttggttgaa tatctgcgtc aagttaatat
      181 ttaacaaata aactcaaaat aattttttat aagggaaaat actagatatt ttagttaagt
      241 gtcaaaaatg tagatgaacg caacgagctt ctcgatagag gttgacttga gaatgacaag
      301 tgactaacat aacttgaatt aagttagttg gagtatttat tatgcaagat ttggaaaatg
      361 gtccattacc gaaaacgttg catacgcaaa ccttagaatt aattgcaaaa gttcttttac
      421 gtaatcccga gtctcttgct tttactttta ccaacgctaa taaaacatta tcggtccatt
      481 ttcaggataa ttctatcaac atcaccgaac tactacaaaa agctaattta aatcatattc
      541 aactggatga agcggatagc gaattatatc ttgaaatttt taacgaggat cccaacacta
      601 cgacatataa agatttaacc gcagcagaaa aaattgtttt acgcgggttt acttccacca
      661 gttattttat caataattta ttgtatggca actataagta tattttagaa tgcggctatc
      721 ttaataatca aaaagtacaa gacattttat tacaaacact ttttgtggct tccggcttga
      781 ataaaataat gcccgaaacc ttatctttta aaacgtatcg tggcgaaggc tcaacgctcg
      841 atccggaaat tgaaaaaaga aaagtctatg ttacagatca agaatactat caagaacgtg
      901 catttaaaag tacaacttct tcttatgaag tagcaaaaag attttctaga aataaagtgc
      961 ttattgaatt tgagcatgcc tatggaaaag atatttcggg aatttcttat tttaaaaatg
     1021 aaaaagaata tttgttgaat gctgagaaaa ttcaatggct atcgcataag caagaaaaag
     1081 aattgaatat ttttaaagct agagtcgtaa atcctttaga tcctattcga gacgctgtta
     1141 ccgatcgtga gatcgatcaa tttttaaaat taaaagaata tgcagaatat cttgatatcg
     1201 aatttgattt tttaaccgat tttaacagag cgcagttgcc accacagcct tcgtggttag
     1261 acgatttatt aagtccgata aacacctgta cagctttgtg ggatagtaca aagaaatttg
     1321 tatatgatac atttgcgcaa gaatacgata cgtatagcga agaagaggat cttagtttta
     1381 gcatttttta agatatgaat cacttttttt taaaaataac tttaaaaact tatttagcta
     1441 aatttatatg gattcccgcc ttcgcgggaa tgacgaagag aatcaagtct gctcgcgcta
     1501 atggcgcata aaaaacaaaa cttcagagtg gattcccgca ttcgcgggaa tgacgcacag
     1561 caatcatttc ctggcgggaa tgcagtacag gaatcatcaa cacttcagag tggttcccgc
     1621 attcacgtga ggatgaattc tcgtaagtct tgtcattccc gcgaaagcgg gaatccagtc
     1681 atctttctta tgttattgta gaaatgtgat ctatccattt tctaagtttg atgcattcgc
     1741 aaaaaatagc acttcaaaat ggatggattc ctgccttcgc gggaatgacg aagagaatca
     1801 agtctgctcg cgctaatggc gcacaaaaaa caaaacttca gagtggattc ccgcgttcgc
     1861 gggaatgacg cataaaaatc ataaagattt ttagactaaa ttcccgcatt caaggaaaga
     1921 cggagggaat cattcccgcg aagcgggaat gacgtacaga aatcaaaggc taactaagtt
     1981 atacccactc ccatctaaat ttcacccaac caccgtgtgc atcgaaatcg tctctaaact
     2041 tgtaatcata gtttatggtg aagtaccaat tatggggaca agctacatct aatccagcgc
     2101 caacgcggaa tgtggtgtca tctggactta aaccaggtgt tacaaaagct ccacctaaac
     2161 caacaaagtt tgcagttgaa atttgcgcgt cgttttcaaa gtcatggaat acttgcgctc
     2221 ttagatgcgg cattaactga acgttgtagt ccatttccat gatgtaacct aaacgcaacc
     2281 caccacccag tacaacttca ttcacatcat catattcaac acttaaattt aatgcgcctg
     2341 ccccggtttc ttctaaacgc ttagagtcta gatgagaata tttgaagtta atatgtggcg
     2401 taataaacca gtgatcgatt aataattcat aaccaagttc tgtaaagaag gtaccttgcc
     2461 atgcgccaaa ttcaccttcg gctcttacgt taataggtgg aacaccagga gagacgatat
     2521 aacggtttgt atcataatca tgatatgcaa cagacaatac tgcatcccaa taaattggag
     2581 gtgttatacg ccagttaccg taaataaatc cattataggt ttgaatgtct aaattgtttc
     2641 cagcagctgc ttttgaatct acatcggtat aggcgtagct aaatccaata ccaattaaaa
     2701 cattacgcca ccattcttta tcaaaaccaa tggcaaagcc cgcattccat gcatcgaagc
     2761 cagcaaaacc gctgcgcgca tcgtggttta catcgttacc aaatatttgg atccaattac
     2821 cagcacccgt atcccaatcc cctgctgcat aattgcccgc ttgcatagag gcaaacatag
     2881 gtgacatcgt ggcgagtctt tgcgaaatat ttaataaaac ttcatcaacc gccaagtgcg
     2941 ataaagtcat tatacttcca tcagatattg gtatgagtgt tgataaagct tgttggaatt
     3001 gtgcagcatc taacgtagaa agactttgaa ttaggggtgc aaattctggg ttattcaata
     3061 aggtatcaaa tgcaatacct tctaagaatc ctgctacacc cgctaagttt ggatctgtcg
     3121 cagtatcagt aaatggagtg cgcgttacgg ttaaaaataa atctgtatta tctgtgctag
     3181 gaacaaagtt ataagttgcg ttattgccaa ttatgactgg aagatctcct gcgtacaccc
     3241 ctccatcaac aactgtaaat tgtttaccag tagcaatgct atctccagaa acgtttacgg
     3301 taactgtgca gtttgtgcag gttgctgtac cgcctatagc taatttacca ttcgatggag
     3361 cattcgtaag cgttgtttgc aagttgccag ttgcactgat aggcgcgtcc ccagtaactg
     3421 ttaaagtatt ggtatttaaa ttaaccgtac catcgataat taaattccct gtaatggtgt
     3481 tagaggcgat tgtatttaac gctgcacctg catttaccgt tgtatcagtc gcaaagatac
     3541 catgtccaag atttaaagtt cctgcattaa cattgacagc ctctaaagaa cctccgagat
     3601 cccctaaatt cccagctgtg gttgagtttc cttggtaagt gacaattcct gttcctgggt
     3661 ttaatgtaaa tacttccgta ttaagattta cacccggctg aacaatagcg ctcccactta
     3721 ctgcattatc aaatcctata ccatttttag tataaacatc accaacaaag gttacagttt
     3781 ttccagcacc ttgtatacct ataccattta tcggaagaag actagttccg atgcttccag
     3841 taacaattga tgtgccttga aaagcaattt gaccttggtt cgcatttata gtttctgcac
     3901 taacccctcc cacatttcca atttggtcac cattattaac tgtcaaaaga aaatcgcctg
     3961 taaataagac gccttccgtt gtacacacgg aaccgggatc actacagttt tcagctcccg
     4021 taataagttc ttgagcatgt attgcattgg aaaaaaatat tgaagaagtt aagaaagcaa
     4081 atgttaaaga ttttttgaat tcgagggcac gcatttagtt agtttcctta cgttacgcag
     4141 gtttttactt aatcacatta ttctacgcaa ttttttcttt tgcaatattg aataatcttt
     4201 tttacaaaca tattcttctg ctgattgaac actcagtaca ttttcggtgc tctttttttg
     4261 caatttttat aaaaaaccca gtaatgtttt tttgcaagtt ccttgcataa atatcacaat
     4321 agttttaata cacccattag atttataatg tttcacagca aaaatagtaa aaaccattgg
     4381 ataacaaaat atttagatac tttgcatcta aaaaacatta ttgcgagtac tgcaaagcgc
     4441 gcaagcgcat ttggcagcga gcaaatagtt attcgatcat tttttccttc gcctctcgca
     4501 catgcagttc aatatatatt caccaatctt tcaggatcct taagtaaaga gttggtttta
     4561 tattgtttaa acaataaaga acatacggat tcgatagaat gtttagtaat tccgattcct
     4621 gaagaagatt taaaagcatt agacaaagca aaaaaaaccc aagattggaa aacatttttt
     4681 gcacgcataa aacatgttgg ccaaaactct gttagagttg gtctacataa tattgtcaca
     4741 ctttcttacg ccgattttgc gacgaatgca ggagcaacta tgggatcatt gcttgcccaa
     4801 ggggtatttt ttggcatggt gggtccccct tcgctgctta ccgctcccct gtattttctt
     4861 accgaaggta gtataaaaag tctaagcgct tttttgggga atcaattgtg taggcaaaat
     4921 acgttacacg cagaggatcc catttttctg gatgccttaa ccacggttct gcaaaaaatg
     4981 cacattcaac aagaagagca agaagattat gagtgtcaaa ccatagatgg tgcagaacaa
     5041 tcagatttat tacaatcgtg ggaattgttt aaaactccca tcccaaaagt acagcaaaac
     5101 gaacaattcg ctgtgatcga cgattataag ccttaaactt attccacttc ttcatccgct
     5161 ggcaattgtc gtgcttcttc ttctaacatc actggaatat cttctctaat aggaaacgcc
     5221 aaacgatcca atttgcaaat gagttctttt tcttgttttt tataaaccag cgggccttta
     5281 cacacaggac acgctaaaat atcaagcaac tttctgtcca ttttctacat cctctagtat
     5341 tcgcgaaata aaagcttctt ctaaagttac ttccaaacgt accacccaaa tcggactacg
     5401 aatttttaaa cctcgacatt ttaccgcatc tttttcggtg ataagtatag gagcattatc
     5461 ttgaaaatag agatctttac tttgaaaaaa atggtgatct gggaagctat gctccaaagg
     5521 taatagtcca tgcgattgaa gcatacgaaa aaatcgattg ggacacgcaa tacctgtgac
     5581 cgcatgcact ttcatccctt taaaatggga taagagtaat tgttcgctcg tatcttgtaa
     5641 tcggtaaaca ccttgttgct cccattgtat agtaaaccct tcgataattt ttacgttatc
     5701 acttaaaata taatcaactt gctttaatcg acttttaggc tctcttaatg ga
//
LOCUS       DASTIB010000175         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1222808, whole genome shotgun sequence.
ACCESSION   DASTIB010000175 DASTIB010000000
VERSION     DASTIB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222808"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFP93_02995"
     CDS             complement(<1..396)
                     /locus_tag="VFP93_02995"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269153.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9843607.1"
                     /translation="MDNTVIFEKSRKHARAHAQIPTLNANDALPVEFLREDSPLLPEL
                     SELEVVRHFTTLSQKNYSIDGQFYPLGSCTMKYNPRGVHQLAGVRPIKNVHPLMPERY
                     SQGFLKCLYELQQYLLSITGMQGISLTPMA"
     gene            complement(400..1761)
                     /gene="gcvPA"
                     /locus_tag="VFP93_03000"
     CDS             complement(400..1761)
                     /gene="gcvPA"
                     /locus_tag="VFP93_03000"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9843608.1"
                     /translation="MPFIPHTEEETQHMLAEIGINNIEALFSEIPTLLKSHSITNIPE
                     GLTEIELFAHVEQELSQDSVALNFAGAGSYEHHIPACVWDLVSRGEFLTAYTPYQAEV
                     SQGTLQCLWEYQTMMASLMGHPVSNASLYDGASALAEAILMGLRVKKAEHPVIFMPDT
                     VHPFYRHTVQALLEFHSVEMVSIPYNCQEGHISLQALEEAYEKTKACTLCIIPQPNFF
                     GILEDASSITDWAHEKNALVIGVVNPTAMAWLKPPGQWGTKGADIACGEGQPLGVPLN
                     RGGPYFGFLTCQSQFIRQLPGRLVGETVDAQNNRAFTLTLQAREQHIRRAKATSNICT
                     NQGLLVTAATIYMSLLGPYGLKKVAQVSHHQSQKLCRSLQALNGFQVIFNRPFFHEIT
                     IRCPVSAPILIERMLEQNILAGISLEETYPELDNALLISVTETKTDLHLQQYLDALKV
                     AVR"
     gene            complement(1771..2160)
                     /gene="gcvH"
                     /locus_tag="VFP93_03005"
     CDS             complement(1771..2160)
                     /gene="gcvH"
                     /locus_tag="VFP93_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005493155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9843609.1"
                     /translation="MSDIPNDLKYTRTHEWTRREDDEIVIVGITDHAQRLLGDVVFVE
                     LPEVGQEVKCGQESGVIESVKAASDLYAPMSGEIIEINEHLHTDPSRVNADPYHDGWL
                     FRLKILDLGEWQDLLDAQEYLETIETE"
     gene            complement(2181..>3065)
                     /gene="gcvT"
                     /locus_tag="VFP93_03010"
     CDS             complement(2181..>3065)
                     /gene="gcvT"
                     /locus_tag="VFP93_03010"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9843610.1"
                     /translation="SKLKNPGTALYSCLLNYEGNILDDLITYKFNQNDFVLIINASQS
                     QKDLNWFIDHKPNHVHIESKSNWSLIALQGPQSRETLSRLYPKHRNQLATLKPFHFFS
                     LEKLTIACTGYTGEDGFEIMGETADLLALWHNLQNVSVPAVGLGARDSLRLEAGLNLY
                     GQDMDETTTPFESNLGWTVDFNDPTRNFIGKDALTKQKSLQIPRKLIGLLVQDAGVLR
                     AHMAVELDQNPALQGVVTSGSFSPILKSSIAFARIPNVAFTQCHVKIRNTWHPAQATT
                     LPFVRLGKVLVQINPLHS"
BASE COUNT          850 a          651 c          640 g          924 t
ORIGIN      
        1 agccatcggt gttaaagaaa ttccttgcat gccggtaatc gataataaat actgttgtag
       61 ttcataaaga cattttaaaa atccttggga ataacgttca ggcattaaag gatgcacatt
      121 ttttatgggt cgaacccctg ccaattgatg cacacctcga ggattatatt tcatcgtgca
      181 agaacctaac ggatagaatt gcccgtcgat ggagtaattt ttttgcgata acgtagtaaa
      241 atgccgtacc acctctaatt ccgataattc aggcagtaac ggcgaatctt cgcgcaaaaa
      301 ttccacaggc aaagcgtcgt ttgcatttag cgtcggtatt tgagcatggg cgcgtgcatg
      361 ttttctagat ttttcaaaaa ttacggtatt atccatgaat taccttactg cgacttttaa
      421 tgcatctaga tactgctgta aatgcaaatc ggttttagtt tctgtgacag agatcaataa
      481 agcattatcg agttcggggt acgtttcttc taaactaatg cccgccaaaa tattttgttc
      541 caacatgcgc tcaatcagta tgggcgcaga aacaggacaa cgtattgtga tttcatgaaa
      601 aaaagggcgg ttaaaaataa cttgaaagcc gtttagggct tgtaagctac gacagagttt
      661 ttggctttgg tggtgggaaa cttgtgcgac tttttttaat ccatagggcc ctagaagact
      721 catatagatt gttgctgccg ttactaataa tccctgattg gtacaaatat tggacgtagc
      781 ttttgcgcgt cgtatatgtt gctcgcgcgc ctgaagtgtt aacgtaaaag cgcgattatt
      841 ctgtgcatcc acggtttccc caacgagtcg ccccggtaat tgtcgaataa attgtgattg
      901 acacgttaag aatccgaaat aagggccacc tcgatttaag gggacaccaa ggggttgacc
      961 ttcgccacag gcaatatcag cgcccttggt tccccattgg ccgggaggct ttaaccacgc
     1021 catcgctgta ggattgacga ccccaatcac cagtgcattt ttttcatgtg cccaatcggt
     1081 gatcgaggat gcatcttcta aaataccaaa aaaatttggc tgtggaataa tgcacagagt
     1141 acatgcttta gttttctcat aagcttcttc taatgcttgt agcgaaatat ggccttcctg
     1201 gcaattatac ggaatggata ccatttctac cgaatggaac tctaacaagg cttgcaccgt
     1261 gtgccgataa aaaggatgca ccgtatctgg cataaagatt actgggtgtt ctgctttttt
     1321 aacgcgcaac cccattaata tggcttccgc taatgcagaa gcaccatcgt acaaggaagc
     1381 attagaaaca gggtgcccca ttaaggaggc catcatggtt tggtattccc ataagcattg
     1441 taatgttcct tgtgaaactt cagcttgata aggcgtatac gcagttaaaa attcgcctct
     1501 ggatactaaa tcccaaacgc atgccgggat atggtgttca tagctgccag ctcctgcgaa
     1561 atttagcgct acgctatcct gactaagttc ttgctcgaca tgtgcaaaca actctatttc
     1621 cgtcaacccc tctgggatgt tggtaatgct atgacttttt aaaagggtag gaatttccga
     1681 aaataacgct tcgatattat ttattcctat ttccgctaac atgtgttgcg tttcttcttc
     1741 ggtgtgcgga ataaaaggca tcctgactcc ttattctgtt tcaatagttt caagatattc
     1801 ttgcgcgtcc agtaaatctt gccattcgcc tagatcaaga atttttagtc gaaacaacca
     1861 accatcatga taaggatctg cgttaacgcg tgaaggatct gtgtgcaggt gttcgttgat
     1921 ttctattatt tcacccgaca taggggcata aagatcggag gctgctttca cggactctat
     1981 aacgcctgac tcttgcccac atttaacttc ttgtcctact tccggcaatt ctacgaacac
     2041 tacatcacct aataagcgtt gagcatgatc ggtgatccca acgatcacga tttcatcgtc
     2101 ttcgcgtcgt gtccattcat gtgttcgcgt atattttaag tcgtttggaa tatcggacat
     2161 ggctccctcc ctatttaaaa ttatgaatgc agtggattta tctgtactag tactttgccc
     2221 aaacgcacaa aaggtagcgt tgtggcttgt gcgggatgcc aggtgtttct tatttttaca
     2281 tggcactgcg taaaagcaac attgggaata cgcgcaaacg ctatagaaga ttttagaata
     2341 ggagaaaaac taccgctggt taccacacct tgtaatgcag gattttggtc taattcaaca
     2401 gccatatgtg cacgtaagac gcctgcatct tgcacaagta acccaattaa tttgcgcgga
     2461 atttgcaaag atttttgttt tgtcagcgcg tctttcccta taaaatttcg cgtaggatcg
     2521 ttaaaatcaa ccgtccagcc taaattagat tcgaaaggag ttgtggtctc atccatatct
     2581 tgcccataca agtttaaacc agcctctaaa cgcaaagaat cacgcgcacc caggccaaca
     2641 gccggtaccg acacattttg taaattatgc caaagcgcta ataaatcggc cgtttccccc
     2701 atgatttcaa agccatcttc tcccgtgtag cccgtgcatg ctatcgtcaa tttttcgaga
     2761 gaaaaaaagt gaaaaggttt aagtgttgct aactgatttc gatgctttgg atataaacgg
     2821 gagagcgttt cgcgcgattg cggtccttgc aaagcaataa gactccaatt ggatttactt
     2881 tcgatgtgta cgtgattggg tttatggtct atgaaccaat taagatcttt ttgcgattgg
     2941 gatgcattta ttattaaaac aaaatcgttt tgattaaatt tataagttat gagatcgtct
     3001 aaaatattac cttcatagtt taataaacaa ctgtacaacg cagttcctgg gtttttaagt
     3061 ttact
//
LOCUS       DASTIB010000176         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1255288, whole genome shotgun sequence.
ACCESSION   DASTIB010000176 DASTIB010000000
VERSION     DASTIB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255288"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1387
                     /gene="kynU"
                     /locus_tag="VFP93_03015"
     CDS             125..1387
                     /gene="kynU"
                     /locus_tag="VFP93_03015"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET9843611.1"
                     /translation="MKGCTQEQATLEHAQFLDKNCALISTREEFNLPPGKMYLCGHSL
                     GPMPKKTNREIDAILNHWATSGVSGYLEGQEAWAYYAESLLENLALLTGAHSRELVIM
                     NSLTVNLHLMLATFYKPTKERYKILVEEGIFPTDKFALESHSKLHDSPPDPLLFVKRE
                     ENGLISTEHILRMIEAHHDNLALVLMPCVQYATGQVINIKSIAESIQSSPIILGVDAA
                     HAVGNIPLQLHDWNVDFGVWCHYKYMNAGPGAVAGCFIHERHVHDVEKFRLGGWWALP
                     SSVRLTMSENFLPDTTARGWQLSCPAILSLAPLKASLELFKAVTLTKLRQKSQKLTEF
                     FVALVNYFNIPCTLVTPLNRDQHGNQLSLTFGNTKQEQVYDALLKANICVDMRKPNLI
                     RITFNPFYNTYSEVFTCVQTLKNIVRVL"
     gene            1384..2274
                     /locus_tag="VFP93_03020"
     CDS             1384..2274
                     /locus_tag="VFP93_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET9843612.1"
                     /translation="MKLPDEIVDCINNIEHKYSVSGQDLRGYLEGLLYAKSLTYWDYI
                     HLETLLSIQQTKTNFLDEKIFIIYHQITELYFSLILHELRDLCENQYSDKSSWMRILS
                     RINRYFRLLIDSFEIMIKGLKKEEFLKFRMSLLPASGFQSVQYRMIELYSTYLYRLVQ
                     PQQRSELKESSLESQFNNLYWKFGNITLKSSEKTLTLQHFEAKYHQFLWELAQKCTHA
                     NMAFLWDHLPPSLQTDEELIKELQLYDAQANEGWRRRHFKAAAFHLHQEPNVIRATGG
                     TNWQEYLPPKKQHIRYFEWS"
BASE COUNT          780 a          426 c          465 g          732 t
ORIGIN      
        1 taggggatga aaccgctaaa ggggaaattc caaaacacaa acaaccaaaa ctagatgaaa
       61 ttgatggact atcggaaatc cccgaactac cggatgaacc agaacccatg ccatagaaaa
      121 ttatatgaaa ggatgcacac aggaacaagc taccctcgaa catgcgcaat ttcttgataa
      181 aaattgcgct ctcatatcca cacgtgaaga atttaatctg cctccgggaa aaatgtattt
      241 gtgtggccat tcgttaggtc cgatgccgaa aaaaacgaat cgtgaaatag atgcaatctt
      301 aaaccattgg gcaacttctg gggtaagtgg ttatttagaa ggtcaagaag cttgggctta
      361 ttatgcagaa tccttattag agaatttggc tctgctgacg ggagctcata gtagagaact
      421 tgtaataatg aactcgctta ctgtcaattt acatcttatg ttggcaactt tttacaagcc
      481 aaccaaagaa agatataaaa ttttagtaga agaaggaata tttcccaccg ataaatttgc
      541 gcttgaatca catagtaaac ttcatgacag ccctcctgat ccattattgt ttgtaaaaag
      601 agaggaaaat ggcttaatct ctaccgaaca cattctcagg atgattgaag cacaccatga
      661 taacttagct ctagtattaa tgccatgtgt acaatatgct acaggacaag ttattaatat
      721 taaatctata gcagaatcaa ttcaatcatc tcctattata cttggggtag atgcagcgca
      781 tgccgtgggt aatatccctt tgcaattaca tgattggaac gtagattttg gagtgtggtg
      841 tcattacaaa tatatgaacg caggtcctgg agctgtagcg ggttgtttta tacatgaacg
      901 tcacgtgcat gatgtggaaa aatttagatt ggggggttgg tgggctttac cgagttctgt
      961 tcggctgact atgtccgaga attttttacc agatactacg gctcgtggct ggcagttaag
     1021 ttgtcctgct atattatcac tagccccgct taaagcttct ttagagttgt ttaaagccgt
     1081 aaccttgacc aaactgcgtc aaaaatctca aaagcttacg gaattcttcg tggctttagt
     1141 aaactatttt aacataccgt gtacactcgt tacgccttta aatagggatc agcatggcaa
     1201 tcaattatcg ttaacttttg ggaatacaaa acaggagcaa gtttatgatg cgttactcaa
     1261 ggctaacatt tgtgtagata tgcgaaagcc gaatctgata cgaattacgt ttaatccttt
     1321 ttataatacg tattcggaag tttttacatg cgtacaaact ttaaaaaata tcgttagagt
     1381 tttatgaaat taccagatga aattgttgat tgcattaaca atattgaaca caaatatagc
     1441 gtttctggcc aagatctgcg cgggtatttg gaggggttgc tttatgcaaa aagtctaacc
     1501 tattgggatt atattcattt agaaacgtta ttaagtattc aacaaacaaa aaccaatttt
     1561 ttggatgaaa aaatatttat aatctatcat caaataacgg agctttattt ttcgcttatt
     1621 ttacatgaac tacgagatct gtgcgaaaat caatattccg ataagagttc gtggatgcgg
     1681 atactctcga ggataaatcg ttattttaga ttgttgatcg atagttttga aatcatgata
     1741 aagggtttaa aaaaagaaga atttttaaaa tttcgcatga gtttattacc ggctagcggt
     1801 tttcaatcgg tgcaataccg aatgattgaa ttgtatagca cctatttata ccgcttagta
     1861 caaccgcaac aacgatccga acttaaagaa agcagtttag agtcacagtt taataactta
     1921 tattggaagt ttggtaatat aacattaaag tcgagtgaaa aaacattaac cttacaacat
     1981 tttgaggcga aatatcacca atttttatgg gagcttgcgc aaaaatgtac gcacgcaaat
     2041 atggcttttt tatgggatca tttacctcca tcgttgcaaa ccgatgaaga acttataaaa
     2101 gaattacaac tatacgatgc acaagccaac gaaggatggc gaagacgtca tttcaaagca
     2161 gctgcgtttc acttacatca agaacccaac gtcattagag caacaggggg gacaaactgg
     2221 caagaatatt tgcccccaaa aaaacaacat atacgatatt ttgaatggtc ttgactatct
     2281 atttttcttg ccaccttttt caaattcttt gttgggctta ctatgacttc ttttaggggc
     2341 tttttgaact tgttgcttta attcccattg ttcttgttcc cgatcatgat tatgatcatg
     2401 cac
//
LOCUS       DASTIB010000177         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_374895, whole genome shotgun sequence.
ACCESSION   DASTIB010000177 DASTIB010000000
VERSION     DASTIB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374895"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFP93_03025"
     CDS             <1..332
                     /locus_tag="VFP93_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9843613.1"
                     /translation="IAIDDFGVGHSCLSYITNLPVNTLKIDRCFVNNISTDIASQKIV
                     QAIISMATSLDLSVVVEGIETKKQEAVVKTLGAKFGQGFYFHKPVTSDEFIQIFVKPD
                     KIKKISF"
     gene            425..>2582
                     /locus_tag="VFP93_03030"
     CDS             425..>2582
                     /locus_tag="VFP93_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843614.1"
                     /translation="MGLEELYTYLSESKIPLAADDIYKIIELRKSIPENLWKNNFHYY
                     LIEHSNQKMWMGDLKKIYYDTLTNYLELNLLSKNKLNLIAMFEVLPKEDFIQTMGEIR
                     VGDFIDEQLKKQPLNPENIGLLLSPELNYTRELGWSSEAVSLLGNMVFSNSADLVPLL
                     LKKYPTINSKDIWAGLYSATSGGLAPNFNKIHQLLIADDHNHLKGNYNTIFTQIFFQG
                     FAKIFESYNKSGELSEKEHITFRECLPILRILLNKGVQLDENTKKWLSTFVKWPLFCE
                     ALPEPKVTDTGYKQFYHVLMTLRKNHLLAHRMDILGESEIEGIKIEMHGSYPQIMMPE
                     ARKSFASFLESHLDEIVKGRVYESDIIDENKFRKAILEAQQDLSAVLMTDKENEITAI
                     QSNNIPVYIPVTVRSQRGFHQIAVSIGNDLCLICDRARDEKSGIEIYKVGTTEATKLE
                     CIKKLGKTVPAARMAPYSYANVISQELASTLNLIPLDYIERSEQYGENCTWNSSAKMI
                     IFSALYLRFYQLAKDNNMSQEEALNFAKVNANPIYKKWSKFDKKQFLINYLNTYLVDE
                     NNKYGPDTTLLSMIYLRNEYKPKYKELTDLLRNSGYINEQTLAQAQKQMYQMAEFIIN
                     DSLPRGFSHENPELMKKMINSLTEYFIRTDKKTVNEVFFVLEEKIKSGRLDEIPTFLD
                     NAMAELEGLQTQNKVGTVLHQYAGKKTAKSQLDLPPK"
BASE COUNT          949 a          427 c          448 g          758 t
ORIGIN      
        1 gaatagccat tgacgatttt ggtgtgggcc attcttgctt aagctacatt accaatttgc
       61 cggttaatac attaaaaatc gatcgctgct ttgtgaataa tatttctaca gatatcgcta
      121 gtcaaaaaat tgtgcaagcc ataatctcca tggctaccag cttagattta agtgtggtcg
      181 tggagggtat tgaaaccaaa aaacaagaag cggtggtaaa gaccttaggc gctaaatttg
      241 gacaaggttt ttattttcac aagccggtta ccagtgatga atttatacaa atttttgtaa
      301 aaccggataa aattaaaaaa atttcgtttt aactacacca tattcctccg caatcgttta
      361 tattgaacct attcaaagta tcagtagtca ctatacatag aaaaccaatc ttgaatgttg
      421 atttatgggt ttagaagaat tatatactta cctttctgag tcaaaaatac ctttagccgc
      481 agatgatatt tacaaaataa tcgaattgcg aaaatccatt cctgaaaatc tttggaaaaa
      541 taattttcac tattatttaa ttgaacatag caaccaaaaa atgtggatgg gtgatcttaa
      601 gaaaatatat tatgacactt taacaaacta tttagaatta aatttactaa gtaaaaacaa
      661 gctgaatctt atagcaatgt ttgaagtatt accaaaagaa gattttatac aaacaatggg
      721 tgaaataagg gttggtgatt ttatcgatga acaactcaaa aaacaacctc taaacccaga
      781 aaatataggg cttttattat ctcctgaatt aaactatacg cgcgaattgg gatggagttc
      841 tgaagctgtc tccctattgg gcaatatggt tttttccaat tctgctgatt tggtaccatt
      901 gcttttgaaa aaatatccca ctataaatag caaagatatt tgggcagggc tttatagcgc
      961 tacctccgga ggtttagcgc caaattttaa caaaatccat cagctcctga ttgcagatga
     1021 tcataaccat cttaaaggaa attataacac tatatttacc caaattttct tccaaggttt
     1081 tgcaaaaata tttgagtctt ataacaaaag cggggaactg tctgaaaaag aacatattac
     1141 atttcgggaa tgtcttccta ttttacgcat attactaaat aagggagtac aactggatga
     1201 aaataccaaa aaatggctat cgacctttgt gaaatggcca cttttttgtg aggcattacc
     1261 cgaacctaaa gtgacagata ctggctataa acaattttat catgtgttaa tgacgctacg
     1321 taaaaatcat ttgttagcac atcggatgga tattttaggt gaatcagaga tagaaggcat
     1381 aaaaatagaa atgcatggca gttacccaca gatcatgatg ccagaggcaa gaaaatcttt
     1441 tgcttctttt ttagaatcac atctggatga aatagtaaaa ggcagagtat atgaatccga
     1501 cataattgac gaaaataaat ttagaaaagc aatattagaa gcacaacaag atttatcagc
     1561 agtattaatg accgataagg aaaatgaaat aaccgcgatc cagtccaata atatacctgt
     1621 ttatatccct gtcactgtta gatctcaacg tggcttccat caaattgctg tatccatagg
     1681 gaatgatttg tgtttaatct gtgatagggc gcgcgatgaa aaaagtggaa ttgagattta
     1741 caaagtggga actactgaag ctacgaaact agaatgcatt aaaaaacttg gcaaaacagt
     1801 gcctgccgca agaatggcgc catatagcta cgcaaacgtc atttcacaag aactcgcgag
     1861 cactttaaat ttaatccctt tagattatat tgaacgctca gaacagtatg gggaaaattg
     1921 tacttggaac tctagtgcaa aaatgattat tttttcggct ttgtacttac gcttttatca
     1981 attagcaaaa gacaataata tgtctcaaga agaggcacta aattttgcaa aagttaatgc
     2041 taatcctatt tacaaaaaat ggtccaagtt tgataaaaag caattcttaa taaattattt
     2101 aaatacgtat ttagtagatg aaaataataa gtatggtcca gataccacac ttctctccat
     2161 gatttattta agaaacgaat ataagcctaa atataaggaa ttaactgatt tgttaagaaa
     2221 cagtggctat ataaacgaac aaaccttagc gcaagcgcaa aagcaaatgt atcaaatggc
     2281 agaatttata ataaatgatt ctctccctcg cggattttcg cacgaaaatc cagagcttat
     2341 gaaaaaaatg ataaattcac ttaccgaata ttttatacga acagataaaa aaaccgttaa
     2401 tgaagtattt ttcgtgcttg aggaaaaaat aaaatcaggg cgcctcgatg aaattccaac
     2461 ttttttagat aatgcaatgg cagagttaga agggctgcaa acacaaaaca aagtcggaac
     2521 agtcttgcat caatatgctg gcaaaaaaac tgcaaaatca caattggatt tgccacccaa
     2581 ag
//
LOCUS       DASTIB010000178         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_497757, whole genome shotgun sequence.
ACCESSION   DASTIB010000178 DASTIB010000000
VERSION     DASTIB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497757"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VFP93_03035"
     CDS             complement(<1..1453)
                     /locus_tag="VFP93_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843615.1"
                     /translation="MNIIEGRLGLSIPIQKQSTSADFGDFKKENIEEWVLGLPQNNLG
                     IMNKALYEFIVELNKTTLSPHVRFETLELILPQVAFVTQSLNKRYMDDNDVPKDKRKK
                     IALLIEILNMEMCLGYKLIIETLGTRLFKKKSVLLKALHRVTIFSLEILQKCYQMYTS
                     APRNLWRELHIIYQYAEKTRLLNKIVEHFDSRSVRHSTIQNAYKHALLLACANPYRLR
                     NQEIFKLNFALEAWAPMLALNKYTPHSKEIFLVDLKDDSPPHYTDLKLPASKSSLSLN
                     FDKINQRMDEILNHARKRDSTLTFTGLPCEFQISITVLKFLKVSWSSFTDRGTNRIDQ
                     KGQVRVTLGLKTTYAAIVHHTKVDNTIGESVEDQENEIIYMDALPLPVNKDQKPSFKL
                     IPAEYVNISPTGFCFEWKEPIPSDLKTGEIIGIEQNPELEGILCIGIVRWIKHMDDET
                     VRCGVKLISPYAIPILTIIPDQAHKLGIKSLLLP"
     gene            complement(1557..2561)
                     /gene="epmB"
                     /locus_tag="VFP93_03040"
     CDS             complement(1557..2561)
                     /gene="epmB"
                     /locus_tag="VFP93_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014291550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HET9843616.1"
                     /translation="MIPELTVKVERHSWQKEMQDTFTSTTELLQFLEIDSRDVLIQPT
                     FPLRVPRTFAKKMEKGNRYDPLLMQVLPLLEESKPTPNFCADPLQETSVNPIPGLLHK
                     FQNRVLLIASKSCAVHCRYCFRKEFPYESNSINRQEWQKCFEYIANDKQITEVILSGG
                     DPLIFKDAHLLWFFKQLSAIAHVRLIRIHTRTPIVIPNRLSPELADILGTVQKRVVIV
                     LHINHPNEICKNLQKNIKYFVHKGITLFNQSVLLKGINDNATILADLSYKMFESHVLP
                     YYLHALDPVTGSAHFQVEDSSMQAIYRELQCILPGYLLPRLVKEMPGLPFKKPLSSFV
                     "
     gene            2618..3187
                     /gene="efp"
                     /locus_tag="VFP93_03045"
     CDS             2618..3187
                     /gene="efp"
                     /locus_tag="VFP93_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9843617.1"
                     /translation="MAIYSTNEFKSGLKVMLDGDPCSIIDNEFVKPGKGQAFNRVKLR
                     NLKSGRVLEKTFKSGDTLEAADVVDMEAQFLYADGERWHFMITDTYEQVEVDKTAAGD
                     SVKWLKEQDICIVTLWNGNPLAVAPPTFVALSITDTDPGLKGDTSGGGTKPATLETGA
                     VVKVPLFVQIGDLIKVDTRTGEYVSRVKE"
     gene            3174..>3690
                     /locus_tag="VFP93_03050"
     CDS             3174..>3690
                     /locus_tag="VFP93_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET9843618.1"
                     /translation="MLKNNWRPTASIDVLKERAKLYQEIGHFFAQRDVWEVETPLLGL
                     TNSPDPHIEPFTVHMKNDRFCLQTSPEFYMKRLLAAGSGSIYQMCKAFRKSEIGRNHN
                     PEFTMLEWYHVGWTHEELIHEVEELLRIILNVSKIMHFTYSALFQEFLAINPLHATEQ
                     ELKQKALALYPH"
BASE COUNT         1144 a          651 c          739 g         1156 t
ORIGIN      
        1 agggtaaaag taacgattta attcctaatt tatgagcttg atccggaatg atggttaaaa
       61 tgggaatggc ataggggctt ataagtttaa caccacaacg tacggtttca tcgtccatgt
      121 gctttatcca acgcacaatg ccaatgcata aaatgccctc tagctcagga ttttgttcaa
      181 tccctataat ttctcctgtt tttaaatcag aaggaatagg ttctttccac tcaaagcaaa
      241 aacctgtcgg gctaatattc acatattccg ctggaatcaa cttaaaagaa ggtttttgat
      301 ctttgttcac gggtaaaggt agtgcatcca tgtaaatgat ttcattttct tgatcttcaa
      361 cactttcccc aatggtgttg tcaactttcg tatgatggac gattgcagca taggtcgttt
      421 ttaagccgag tgtaacgcgc acttgtcctt tctgatcgat acggtttgtg ccgcgatcgg
      481 taaagcttga ccatgatact tttaaaaatt ttaatacggt tatagaaatt tggaattcac
      541 acggcaagcc cgtaaaagtt aacgtagaat cacgcttacg ggcatggttg agaatttcat
      601 ccatgcgttg attaatttta tcgaagttta aactcaaaga agatttactg gcgggtagct
      661 ttaaatcggt ataatgcggt ggcgaatcat cttttaaatc caccaaaaat atttctttgc
      721 tgtgtggggt atatttattt aaagctaaca taggtgccca agcttcgagt gcaaaattta
      781 gcttaaaaat ttcttgatta cgtaaccggt atgggttagc acatgctaaa agtagagcat
      841 gtttataagc attttgaatg gtgctatgtc ttaccgaacg ggagtcaaag tgttccacga
      901 ttttattcaa aaggcgtgtt ttttctgcat attgatagat aatatgtaat tcccgccata
      961 aatttcgagg tgcacttgta tacatttgat aacatttttg taaaatttct agggaaaaaa
     1021 tggtaacgcg atgcaaagcc tttaaaagaa cggatttttt cttaaataag cgcgtcccta
     1081 aagtttcaat aatgagctta tagccaagac acatttccat atttaaaatt tcaattaaga
     1141 gtgcgatttt ttttcgttta tcttttggaa catcattatc gtccatgtaa cgcttattta
     1201 aagattgggt gacaaaagca acttggggga ggatcaattc caaggtttca aaacgcacgt
     1261 gaggagaaag tgttgtttta tttaattcca caataaactc ataaagggct ttattcatta
     1321 tccccaaatt attttgtggt aatccaagca cccactcttc gatattttct tttttaaaat
     1381 caccaaaatc agcactggta gattgctttt ggatgggtat actcaggcct agtcggccct
     1441 ctattatatt cataggtcaa gcatactcct catgcctttc tacaccattg aatttaacca
     1501 gtggttaaag ggttccttca tatgttctat ttcgataaag cgcaaaaatt ctttaattaa
     1561 acaaaagacg ataaaggttt tttaaaagga agacctggca tttctttgac aagtcgaggc
     1621 aaaagataac ccggtaatat acattgcagt tcacggtaga ttgcttgcat actgctatct
     1681 tctacttgga aatgtgcgct tcccgttacg ggatcgagag catgaaggta atagggaaga
     1741 acatgggatt caaacatttt ataagacaga tcggcaagaa tggttgcatt atcgttaatc
     1801 ccttttaaaa gaacactctg attaaacagt gtaatgcctt tatgtacaaa atattttata
     1861 tttttttgca gatttttaca gatctcgttc gggtgattga tgtgtaaaac aatgactaca
     1921 cgtttttgca cagtgcctaa aatatctgcc agctcaggag aaagtcgatt aggtataaca
     1981 atgggagtgc gtgtatgaat gcgaattaaa cgaacatgcg caatagcact taattgtttg
     2041 aagaaccaaa gcaaatgggc atctttaaaa atcagcgggt cgccaccact taaaataact
     2101 tcagtaattt gtttatcatt cgcaatatat tcaaaacatt tttgccattc ttgacgatta
     2161 atactattag attcataagg aaattccttc ctaaaacaat aacgacaatg gactgcgcaa
     2221 gatttcgatg caattaatag cactctattt tgaaatttat gtaagagacc cggaatagga
     2281 tttaccgaag tttcttgaag cggatctgcg caaaaatttg gtgttggttt actttcttct
     2341 aggaggggta acacttgcat caataaagga tcatagcgat tacctttttc catttttttt
     2401 gcaaaagtgc gtggaactct aagcggaaaa gttggctgga ttagcacatc acgactatca
     2461 atttctaaaa attgaagcag ttcagttgtg gaagtaaaag tatcttgcat ttctttttgc
     2521 caagaatgcc tctcaacctt tacggttaat tcaggtatca taaacgactg ttttcgtaga
     2581 ttgattggcg attctagcag aatttaggaa aaaacaaatg gcaatttata gcaccaatga
     2641 atttaaaagc ggcttgaagg taatgctcga cggagaccct tgcagtatta ttgacaatga
     2701 atttgtgaag cccggtaaag gtcaggcctt caatcgtgtt aagctaagaa acttaaaatc
     2761 agggcgcgtc ttggaaaaaa catttaaatc aggcgatact ctggaagctg ctgatgtagt
     2821 cgacatggag gcccaatttt tatatgccga tggggaacgc tggcatttta tgattacgga
     2881 tacatatgag caagttgaag tagataaaac tgctgcagga gattcagtta aatggcttaa
     2941 ggagcaggac atatgcatcg ttaccttatg gaatggtaat ccgttagccg tcgctccacc
     3001 cacatttgtt gcattaagta ttacagatac ggatccaggt ttaaaaggag acacgtctgg
     3061 tggtggtacg aaacctgcaa cattagaaac gggtgctgtg gtaaaagtac ctctttttgt
     3121 gcaaattgga gatttgatta aagtggatac ccgaactggg gagtatgtat cgcgtgttaa
     3181 agaataattg gcgtcctacc gcttccatcg acgttttaaa agaacgcgcg aaactttatc
     3241 aagaaattgg ccattttttt gctcagcgtg atgtatggga ggtagaaact cctttattag
     3301 ggctaaccaa tagccctgat ccgcatattg aaccttttac agtgcatatg aaaaacgacc
     3361 gtttttgtct gcaaacttca ccagaatttt acatgaaacg tttgttggct gcagggtctg
     3421 gatccattta tcaaatgtgt aaagcgtttc gtaaatcgga aattggacga aaccacaacc
     3481 ccgaatttac catgctagaa tggtatcatg tagggtggac gcatgaagaa cttattcatg
     3541 aagtggaaga acttttaaga attatattaa acgtttctaa gatcatgcat tttacttaca
     3601 gtgcattatt ccaagaattt ttagcaatta accctttaca tgctacagaa caagagctta
     3661 agcaaaaagc actcgctttg tatccccatt
//
LOCUS       DASTIB010000179         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_939483, whole genome shotgun sequence.
ACCESSION   DASTIB010000179 DASTIB010000000
VERSION     DASTIB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939483"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFP93_03055"
     CDS             <1..645
                     /locus_tag="VFP93_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843619.1"
                     /translation="VLLSIAKARSAIAVINKDYAEDKPVDPIKIKEIEKHLQTLENNI
                     QHASSVLPPTRLWAAGFYQEMGLVEHLKNLAKSLTKQDMFYNEILRMLSETIIYGPQT
                     YDENNDPDFSKKAHKHKLLEAFDYSLEVQDSKELRKNIAFNFVYGVDNKCAGFPTQSN
                     EPLLDHLEGGKETCLYAIKALRQKVKLQKSSEEKDKKIKQLEAEIAAMKAKQHI"
     gene            861..1874
                     /locus_tag="VFP93_03060"
     CDS             861..1874
                     /locus_tag="VFP93_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET9843620.1"
                     /translation="MKRAIDNDLIKNAKKSRASADSLVESNKTEPLTQNIEPLSPLFN
                     TVKKKNEEEIVITDPMLFRVSKDNKSFFLLGTNHSLSFEAFTPEVKKAMLDCEKLILE
                     RANMDNPKKEFEDFIEGNLASILRTGTDSAQYIQLPEKEKEIVVNTLYVFKNALNKFN
                     KDLSDLKDEIVIGAFIGVCTQISGEHSMELDLTNAFKKMNKSIEGLEQSKDLLEQVVE
                     FTTWMKKTDLVMAINTFNNSKNEQCENHKTYAQGSSLRKSKYNSDAIENRNAKWFTVI
                     LAEVNKSNNSLLAVGHSHLTNPGNGLLEWLENMGFNLEKCDKEANFSPYSWKDQKSDW
                     RIM"
     gene            1974..>2941
                     /locus_tag="VFP93_03065"
     CDS             1974..>2941
                     /locus_tag="VFP93_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843621.1"
                     /translation="MLKKRSFASNNLKILPTLTFKSEQIAPEKIIFMHVPKTGGTNLH
                     YLAQALEYTGKKDFYYYRFAVPRKENVSPCLLYPGWRGGLDTIEATNPLMCATFNFIS
                     GHFPFDLNALPWNNFKFITLIRNPIDRALSLTNFDIQRGYIGKSDALNYALKCEIDNP
                     QTRAIAGAEWMFGICTQETLDRAKSNIENYFMLAGVTEDTNGFMQVLASILRWKPCAI
                     VQSQVTAEKVLPTFPDDVVKSLKEKYKYDLELYTWVKERWEAWKARNIVEVNFIPYDL
                     KLMCITHEFASTREPVFMDQEEIMKHNNHCEKEYSPIQQIENSQDV"
BASE COUNT         1072 a          459 c          542 g          868 t
ORIGIN      
        1 gtgctcctct cgatagcaaa agctagaagc gccatcgcag ttattaataa agattatgct
       61 gaagataagc cagtggatcc tataaaaata aaagagatcg aaaaacatct tcaaacttta
      121 gaaaataaca ttcaacatgc cagcagcgta ctacccccaa cccgtttatg ggcggcaggg
      181 ttttatcagg aaatgggttt ggtggagcat ttaaaaaact tggcaaaaag tctcacaaag
      241 caagacatgt tttataatga aatattgcga atgttgtccg aaaccattat ttatggtcca
      301 caaacttatg atgaaaataa tgatcccgat tttagtaaga aagcccataa acacaaattg
      361 ctcgaagctt tcgattattc tttagaagtt caagatagca aagaattacg aaaaaatatt
      421 gcttttaatt tcgtgtatgg agttgacaat aagtgcgctg gtttcccaac acaatccaat
      481 gagccattgc tcgatcattt agaaggtggc aaagaaacgt gcttgtatgc aattaaggct
      541 ttgcgtcaaa aagttaaatt acaaaaatca tcagaagaga aagataaaaa aataaagcaa
      601 ctcgaagcgg aaattgcagc tatgaaagcc aaacaacata tatagcttat aagcattgac
      661 aaggaatgtc tccagagaaa gcgtttttcc ttttacacca ataacgcaca ataagttgaa
      721 aaaaatcact tattagggtt aaggattttg ccttatagtt gatatttttt tatcttatcg
      781 tcaataattt acatgctatc tttttgcgat accaacgtga atatattttt attagaatcc
      841 tctttttttg gattaaaaat atgaaaagag ctatagataa cgatttaata aaaaatgcta
      901 aaaagagtag ggcttctgcc gatagtttgg tagagtctaa taagaccgag cctttaacac
      961 aaaatattga gcctttgagt cccctattta acactgtcaa gaaaaagaac gaagaggaaa
     1021 ttgtcattac cgatcccatg ttgtttcgcg tgtcaaagga caataaaagt ttttttcttt
     1081 taggtacaaa tcactcttta agttttgaag cttttacacc agaagtaaaa aaggcaatgt
     1141 tagattgcga aaaattaatt ttagaaagag caaatatgga taatcctaaa aaagaatttg
     1201 aggattttat agaaggaaac ttggcaagta tattgcgtac gggaaccgac agtgctcaat
     1261 atatacaatt gcctgaaaaa gaaaaagaaa ttgtggtaaa cacgttgtat gtgtttaaaa
     1321 atgctttaaa caaatttaac aaagacttaa gcgatttaaa ggatgaaatt gtcattggtg
     1381 cttttattgg agtgtgtacg caaataagcg gagagcattc catggaattg gatttgacaa
     1441 atgcttttaa aaaaatgaat aaatccattg aaggtttaga gcaaagtaaa gatctgttag
     1501 aacaagtagt tgaatttaca acatggatga aaaaaactga tttagtgatg gctataaata
     1561 catttaataa ttcaaaaaat gaacaatgtg aaaaccataa aacttatgcg caaggatcta
     1621 gtttaagaaa gtcaaaatac aattcggacg caatagaaaa ccgtaatgcc aaatggttta
     1681 ctgtaatatt agcggaagtc aacaaatcaa acaattctct cctagcagtg gggcattctc
     1741 atctaacgaa tccaggtaat ggtttattag aatggcttga aaatatggga tttaacttag
     1801 aaaaatgcga taaagaagct aatttttcgc cttatagctg gaaagatcaa aaatcagatt
     1861 ggaggatcat gtaataaatt tttgtcaagg aagacaaaat atcaaaaaaa ttaactattg
     1921 ctgtttcttt gtaatatatt ataattgtta tttcactata tttaatgatt tatatgctta
     1981 aaaagcgttc ctttgcatca aacaacttaa aaatattgcc gactttaact ttcaaaagcg
     2041 aacaaattgc acccgaaaaa ataatattta tgcatgtgcc gaaaacgggt ggcacgaatt
     2101 tgcattatct cgcacaggca cttgaatata cagggaaaaa agacttttat tattatcgtt
     2161 ttgcagtgcc gcgcaaagaa aatgtttctc cttgtttact ttatccagga tggagggggg
     2221 gcttagatac tattgaagca acaaacccct taatgtgcgc aacttttaat tttataagtg
     2281 gtcattttcc atttgattta aacgcattac cctggaataa ctttaaattt attacattaa
     2341 tccgcaaccc gatcgatcgt gctctctcat taacaaattt tgatatccag agaggttata
     2401 ttggaaaatc agacgcctta aattatgcgc ttaagtgtga aatagataat ccgcaaacgc
     2461 gcgcgattgc tggagccgag tggatgtttg gcatttgtac gcaagaaaca ctggatcgtg
     2521 ccaaatctaa tattgaaaat tattttatgt tagcaggagt tacagaggat actaatggct
     2581 ttatgcaagt tctcgcttcc atcctacgtt ggaagccatg cgctatagtt caatcacaag
     2641 tcactgctga aaaggtttta ccaacatttc ccgatgatgt tgtgaagagt ttaaaagaaa
     2701 aatataaata tgatttggag ttgtatactt gggtaaagga acgctgggaa gcttggaaag
     2761 cccgcaacat agtagaggtg aactttattc cctacgattt aaagcttatg tgcataacgc
     2821 atgaatttgc ttcaacgcga gaacctgttt ttatggatca ggaagaaatc atgaagcata
     2881 acaatcattg cgaaaaagaa tattcaccca ttcaacaaat agagaattcc caagatgttt
     2941 t
//
LOCUS       DASTIB010000180         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_658873, whole genome shotgun sequence.
ACCESSION   DASTIB010000180 DASTIB010000000
VERSION     DASTIB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658873"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFP93_03070"
     CDS             complement(<1..358)
                     /locus_tag="VFP93_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843622.1"
                     /translation="MSLSFSSQTFTHEALQNLIKRIGTQNQCMILRGRQTGWNPFYFA
                     AKHLRLLYLRIIRWGWDLGPEQPIASLTLKSVRFSPGQCNALFKTLSPQVQKKIALID
                     VNIDSQSLNTLIQQLIE"
     gene            458..946
                     /locus_tag="VFP93_03075"
     CDS             458..946
                     /locus_tag="VFP93_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET9843623.1"
                     /translation="MKTSKPFLILFLWIFVIATLHAAELYKVISVTDGDTLKILVNDK
                     PEKIRLLYIDAPELGQPYGKKAKQALSDKIYFKYVQIKREGRDQFGRTLGIIYVDQEN
                     INLSMIEDGHAWVYRYYKADPLFLVAEKAARFHKRGLWSLPKEQRQPPWVWRKNAWKK
                     RK"
     gene            986..1576
                     /locus_tag="VFP93_03080"
     CDS             986..1576
                     /locus_tag="VFP93_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9843624.1"
                     /translation="MRFDYIRTQRLIIRIPTIVDAKIMHDALKKSIKDLKQWMPWAQE
                     LASLEDTRYFLRQAETVWTGPLEEHVERSLIIMDVSNKQFVGSTGIKPLNLLVPSFEV
                     GYWVNQEFAGKGFITEAVNALSRFLFGELHANRIEIHSEAKNVNSCKVAERLNFTLEG
                     ILKNHRLTADNKHLTDTKLYAQINVEALPLLSVAWK"
     gene            1576..>2447
                     /locus_tag="VFP93_03085"
     CDS             1576..>2447
                     /locus_tag="VFP93_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843625.1"
                     /translation="MSAPLFFHFNQYGTALRYTLAKLLFDQGWQLADCAEKANFSDHN
                     LSLGDDISIVLEYKHLLALLAKHFFPNKMPQTFNIDDHNYHDVLKRVTKQKQQTPWIL
                     KPAFLNNGDYIYIFENLAQLRAHYSNPARLSGMHVLQKYIINPHLWEGSKYTFRLPVI
                     ATNYAGVFLYQHGYINISPLSYSLDFKPELKRKHITNYVIEGKISGIQQEATFSVPNF
                     SRIFSQMVAIVQAVFQAVLAVFPRYLQINNTQIFEIFGFDFLLDANNKLWLLEINQGP
                     DFPLDTDHPLYKNL"
BASE COUNT          798 a          420 c          467 g          762 t
ORIGIN      
        1 gttcaattaa ttgttgaatc aacgtgttca aactttgaga gtctatatta acgtctatga
       61 gggcaatttt tttttgaacc tgtggactaa gggttttaaa aagcgcattg cattgaccgg
      121 gtgagaaccg tacagatttt aaggtgagcg aagctattgg ttgttctgga cctaaatccc
      181 agccccatct tattatacga aggtataata atcgcaagtg tttggcagca aaataaaagg
      241 gattccatcc agtttgacgt ccccttagga tcatgcattg gttttgtgta ccaatacgtt
      301 taattaaatt ctgcagcgct tcatgggtaa aggtttgaga cgaaaacgat aaagacatac
      361 acaatactca ataatataaa gttggaagtc taaaaaaata tttttaacac aatatgcgtg
      421 agataacaac ttatcacgac aaagttaatt taatagcatg aaaactagta aaccattttt
      481 aattttattt ctttggattt ttgttatagc aacgctacac gctgcagagc tatacaaagt
      541 tatatctgta actgatggtg ataccttaaa aatattagtt aacgataaac ctgaaaaaat
      601 tcgcctattg tatattgatg ccccggaatt aggccaacct tatggtaaaa aagctaagca
      661 agcattatct gacaaaattt attttaaata tgtgcaaata aaacgcgaag gacgtgatca
      721 attcggaaga actttgggaa ttatttatgt agatcaggaa aatataaatt tatctatgat
      781 agaagatggc catgcatggg tatatcgcta ttacaaagca gatcccctat tcctcgtagc
      841 agaaaaagct gcaagattcc ataaacgtgg attatggagt ctgcccaaag aacaaagaca
      901 acccccttgg gtgtggcgca aaaatgcatg gaaaaaaaga aaataatgtg gatacgtatt
      961 agtaggatgc tttggaagga aagctatgcg atttgattat attcgtaccc aacggcttat
     1021 cattcgaatc ccaacgattg ttgatgcaaa gatcatgcat gatgctttaa aaaagagcat
     1081 aaaagatttg aaacaatgga tgccttgggc ccaagaattg gcttcgttgg aagatacgcg
     1141 ttatttttta cggcaagcag aaaccgtctg gactggccct ctagaagaac atgtcgaaag
     1201 atctttgatt attatggatg tgagcaataa acaatttgta ggatctacgg gcatcaagcc
     1261 gttaaatctt ttagtgccta gctttgaagt gggatattgg gtaaaccaag aatttgcagg
     1321 gaagggattt atcacagaag cagtcaacgc tttgtcccgt tttttatttg gtgagcttca
     1381 tgcgaatcgc atagagattc actcggaagc aaagaatgta aatagttgta aagttgctga
     1441 aaggttgaat tttactttag aaggaatttt aaaaaatcat cgtttaacag ccgataataa
     1501 acacctaacg gataccaaac tttatgcgca gattaacgtt gaagcactac cgctcctgtc
     1561 ggtagcgtgg aaataatgtc tgcgccactt ttttttcatt ttaatcaata tggcacggca
     1621 ttacgttata ccctggcaaa attacttttt gaccaaggat ggcagcttgc cgattgtgca
     1681 gaaaaagcaa actttagcga tcacaattta tctttgggcg atgatatttc cattgtgttg
     1741 gaatataaac acttacttgc gttactagct aaacattttt tcccgaataa aatgccgcaa
     1801 acctttaata tcgatgatca caattatcat gatgttctta agcgggtaac gaaacagaaa
     1861 caacaaactc cttggatttt aaagcctgca tttttaaata atggagacta tatttatatt
     1921 tttgaaaatt tggcgcaatt aagagcacat tattctaatc ctgcaagact gtccggcatg
     1981 cacgtgctgc aaaaatatat tattaatccg catttatggg aaggttccaa atatacgttc
     2041 cgccttcctg ttattgctac caattatgcg ggtgtttttt tatatcagca tggttatatc
     2101 aatatttcac ctctcagtta ttctcttgat tttaaaccag aattaaaacg taaacatatt
     2161 accaactacg tgatagaagg aaaaatttct ggaattcaac aggaagcaac tttttctgtg
     2221 ccaaactttt ctcgcatttt ttcccaaatg gtagctatag tccaagcggt ttttcaagcc
     2281 gtattagctg tgttcccgcg ttatctgcaa ataaacaata cacaaatatt tgaaattttt
     2341 ggcttcgatt ttttgttaga tgctaataat aaactttggc ttttggagat taatcaaggc
     2401 cctgattttc cgttggatac ggatcaccct ctttataaaa atttgtg
//
LOCUS       DASTIB010000181         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_883975, whole genome shotgun sequence.
ACCESSION   DASTIB010000181 DASTIB010000000
VERSION     DASTIB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883975"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..764
                     /locus_tag="VFP93_03090"
     CDS             318..764
                     /locus_tag="VFP93_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843626.1"
                     /translation="MIYFPATNIIEQIRIGKFDLFRDLIEDNAIQMVDFSYKAIDAQV
                     APLIAQALEGLSVRCLNLGFNQIGPLGVIELAEGLKQSSIVELDLSRNGIGLAVVDLI
                     TKLKNTSVKAVKLAYNHIGVQGAKAVRPILLASDIKVDLSHNYIWA"
     gene            complement(749..1408)
                     /gene="nth"
                     /locus_tag="VFP93_03095"
     CDS             complement(749..1408)
                     /gene="nth"
                     /locus_tag="VFP93_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9843627.1"
                     /translation="MNAEKILNIFKIFKKINPNPTTELHYHSPFELLIAVILSAQALD
                     SSVNKATKELFKKANTPKKMLKLGEEGLKAFIKSIGLFNTKAKNILKTCKILVEQHQE
                     KVPAQREDLEALPGVGRKTANVILNTAFGMPTIAVDTHIFRVANRTKIAEGKNPFAVE
                     QKLLEVIPKKYLHDAHHWLILHGRYICVARNPKCHICPIYTFCEYPQKEEQKQKLQAQ
                     I"
     gene            complement(1398..2024)
                     /locus_tag="VFP93_03100"
     CDS             complement(1398..2024)
                     /locus_tag="VFP93_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HET9843628.1"
                     /translation="MVRKNIPTLIAQVEATLPQTQCERCGYKGCEPYAHAIVRNGEKT
                     NLCHPGGQKTATAIATLMHQPAQNVDKEPESKLWALIVEADCIGCTKCIQVCPTDAIL
                     GGPKLLHTVLKSECTGCELCLKVCPVDCIEMQPDAREENPSILCAQASYAKERYHARK
                     DRLDQAIPKKLIAMTTTSSHHKKETIQAAIKRVQEKRLLAQKNDTYEC"
     gene            complement(2024..>2169)
                     /locus_tag="VFP93_03105"
     CDS             complement(2024..>2169)
                     /locus_tag="VFP93_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843629.1"
                     /translation="TVMVANLAPRKMRFGVSAGMVLAAGPGGKDLWILEPQAGALPGM
                     RVK"
BASE COUNT          600 a          414 c          428 g          727 t
ORIGIN      
        1 ttaagcgttt tcgtatattt ttcttctaaa taacttcgca tcgctgttag aaatttggtg
       61 tgcatacaaa ttctgcccaa aaagtaggct attatcgttg acgtttttta aaaggcaaat
      121 gtgacggatg aaaggagtta aagaggcgat aaaagtagaa ctttatgtta gttaaattta
      181 attataacta ttttatctat ttgcttactc actttaattc cttgcttgtt gtttcaagct
      241 aattatttta gtattagatg tacatggggt agccagagtt cttacttttg gcaagaagtt
      301 attctacgtg agtctatatg atttattttc cggcaaccaa catcatagaa caaatacgca
      361 ttggcaaatt tgatctattc cgcgatttaa ttgaagataa tgctatccaa atggtcgact
      421 tctcttataa agccattgat gcacaggtgg cacccctgat agcgcaagcg ttagaaggct
      481 taagtgttcg gtgtttaaat ttaggtttta accaaattgg cccgttaggt gtcatagaat
      541 tggcagaagg tttaaagcaa agttcgatag tggaactcga tttatctcgc aatggtatag
      601 gattagccgt tgtcgatctt attactaaac taaaaaatac ttcggtgaag gcggtaaagt
      661 tagcatataa ccatatcggc gtgcagggtg ccaaagcagt acgccccatt ttattagcaa
      721 gtgatattaa agtagattta tcccacaact atatttgggc ttgaagtttt tgtttttgct
      781 cttctttttg tggatactca caaaaagtat agataggaca aatgtgacat tttggattac
      841 gtgccacaca aatgtagcgg ccatggagaa ttagccaatg gtgtgcatcg tgtagatatt
      901 tcttaggtat tacctcaagt aatttttgct caactgcaaa gggattttta ccttctgcaa
      961 tttttgtacg attagcaact cgaaaaatgt gcgtatcaac cgcaatggta ggcatgccaa
     1021 aggccgtatt taatatcaca ttcgcagttt ttcgtcctac gcctggtaac gcttctaaat
     1081 cttcgcgctg agcaggaact ttttcttgat gttgttctac aagaatttta cacgttttaa
     1141 gaatattttt tgctttagtg ttaaaaagac cgatagattt aatgaaagct tttaaacctt
     1201 cctcccccag ttttaacatt ttttttggcg tatttgcttt cttaaaaagc tctttcgttg
     1261 ccttatttac gctcgaatca agcgcttggg cagataatat aacggctatt aataactcaa
     1321 aaggtgaatg gtaatgtaat tccgtcgtag gattaggatt aattttttta aagattttaa
     1381 atatgttaag gattttttca gcattcatag gtgtcatttt tttgggctag aagacgcttc
     1441 tcttgcactc gcttaatagc ggcctggata gtttcttttt tatggtgcga agaggttgtt
     1501 gtcatggcta tcaacttttt aggaatagct tggtctaatc gatctttgcg cgcatgataa
     1561 cgttcttttg catacgaagc ttgtgcacac aaaatactag gattttcttc gcgtgcatca
     1621 ggttgcattt caatacaatc caccgggcac actttaagac aaagttcaca acccgtacat
     1681 tcgcttttaa gtacggtatg caaaagtttt ggaccaccca ggatcgcatc ggtcggacaa
     1741 acttggatgc atttggtgca tccgatacaa tcagcctcaa cgatcaacgc ccataacttg
     1801 ctctcaggtt ctttatcgac attttgagcg ggttgatgca ttaaagtggc tattgcagtg
     1861 gcagtttttt gcccaccagg atggcataaa ttggtttttt ctccattgcg cacgatagca
     1921 tgcgcatagg gttcacaacc tttataacca catctctcac actgcgtttg cggcaacgtc
     1981 gcttcaactt gcgcaataag tgtaggaatg ttctttctta ccactatttc actcgcatgc
     2041 caggtaatgc gcctgcttgt ggttctaaaa tccacaaatc cttaccacct ggtcctgcag
     2101 caagtaccat gcctgcagaa acaccaaaac gcatttttct tggcgctaaa ttggcaacca
     2161 tcacagtat
//
LOCUS       DASTIB010000182         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_185858, whole genome shotgun sequence.
ACCESSION   DASTIB010000182 DASTIB010000000
VERSION     DASTIB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185858"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFP93_03110"
     CDS             complement(<1..278)
                     /locus_tag="VFP93_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HET9843630.1"
                     /translation="MKKNRPHNEAWWALVIQGVSLILLTPIVWFVKDEFVAGSMLLGG
                     LACLIPNVCLYANVFRYFGAQHAHKMVRALYFGELVKLFLTAGIFAFLA"
     gene            complement(349..1263)
                     /locus_tag="VFP93_03115"
     CDS             complement(349..1263)
                     /locus_tag="VFP93_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843631.1"
                     /translation="MRYIIVNLQDFIEVNLDTAFDVIAEHGACYVGAKAIWYAFRKSE
                     SFFNHQEMLLKGQLTKAEFIKRNQNFIASFQMSEKEFISAWNRALCFSPNFLELLTFQ
                     EINQNSHCRFIFVADINRIHFSFIKNKFQLENRSNEYYFSYDFGVTGNDLLVKALADI
                     RRKSPRADIAIAYKPVEYPGYFSWILNPKKNILYRENESKHKILQRLASEYRATLISW
                     SAADSTSIIKFDFCVNLQKWLDSGVAHMHFDAIDLPEKVNDLTFALDKLKVTPDSSPP
                     IQARNSPKNSEKIRQKTQINSGQLAPKH"
     gene            complement(1269..2159)
                     /locus_tag="VFP93_03120"
     CDS             complement(1269..2159)
                     /locus_tag="VFP93_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9843632.1"
                     /translation="MAKNSRLGKGLNELLSVSVLAEGRETSDDTESIVKTPMEVFKNV
                     PIEQVKPGPFQPRLGISSEGIEQLAESMRAQGILQPILVRKKSDDHYEIVAGERRWRA
                     AQLAGIYTIPVIIKEMSEETAFAVALIENIQRENLNPIEEARGLKRLADEINLTHLQV
                     AEAVGKSRTSVTNLLRLLTLNDDVQLLLEQGQLDMGHARALLALKGPIQSQVAKTVVS
                     RNLSVRETERLVNKLQENRSEKVPAVDDPNIKRLQQDLSEKLGAAVKINHSARNGQGM
                     VMIRYNDVDQLEGILSHFKL"
     gene            complement(2167..2985)
                     /locus_tag="VFP93_03125"
     CDS             complement(2167..2985)
                     /locus_tag="VFP93_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9843633.1"
                     /translation="MAQRIYAFANQKGGVGKTTSCVNLAASLVVLNQRVLLLDMDPQG
                     NATMGSGVDKNAVEKSMNDLLLQQVSMDEAIIRQTPAGYDLVAANCDLTAAEVALLKK
                     GKQERILADALAPFRDQYDFILLDCPPSLNMLTVNALTAADAVIIAMQCEYYALEGLS
                     DLLNTISQLQATLNPRLYIGGIIRTLYDGRNRLSSEVSNQLIEHFGNKVFQTVIPRNI
                     RLAEAPSYGLPIILYEKNSRGSSAYLTLGNELLRRHTTANSKKELNQKEAALEI"
     gene            complement(3006..>3355)
                     /locus_tag="VFP93_03130"
     CDS             complement(3006..>3355)
                     /locus_tag="VFP93_03130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET9843634.1"
                     /translation="LLDSNEKRQIFLKQVVHQLHLPNVQLYCTRVENFNASFKFSCIV
                     ARAFKPLAEMLRLVQHLLSSDGSIVAMKGKIEEKELKEIDPKFKIDRIITLQNTSQAP
                     QRHLIIIKFEQTT"
BASE COUNT          967 a          731 c          607 g         1050 t
ORIGIN      
        1 gcaagaaaag caaatatgcc agctgtcaaa aacaacttaa ctagctcacc aaaatataag
       61 gctcttacca ttttatgagc atgttgggca ccaaagtatc gaaatacatt agcgtataga
      121 caaacgttgg gaataagaca ggctaatcct cctaagagca ttgagcctgc aacaaattca
      181 tctttgacaa accatacaat cggtgtcaaa agaattagac tcactccctg aatgaccagc
      241 gcccaccatg cctcattatg aggtctattt tttttcattt atcgctcggc tgaacaaata
      301 gttatttaat gccaagcgcg ccaagtatac tgggctacgc accattaatc aatgttttgg
      361 ggccagttgt ccagaattaa tctgagtctt ttgcctaatt ttttcagaat tttttggtga
      421 gtttctagct tgaatgggcg gcgaggaatc aggagtaact ttaagtttat caagtgcaaa
      481 ggttaagtca ttgacttttt ctggcaaatc aatcgcatca aagtgcatgt gcgcaacccc
      541 cgaatccagc catttttgca agttgacaca aaaatcgaat ttgatgatgg atgtagaatc
      601 tgccgcagac caactgatta atgtcgcacg atactccgac gccaagcgct gtaagatttt
      661 atgcttggat tcattttctc gatagagaat atttttttta ggatttaaga tccaggaaaa
      721 atagcctgga tattccacgg gtttataagc aattgcaata tcggctcgag gcgattttct
      781 tcgaatatcc gctaatgctt tgactaataa gtcattgccc gtaacgccga aatcatagga
      841 aaaataatac tcattcgacc tattctctag ttgaaatttg ttctttataa aagaaaaatg
      901 tatacgatta atatcagcaa cgaatataaa cctacaatga gaattttggt ttatttcttg
      961 aaaagtcaaa agctctaaaa aattaggaga aaaacacaat gcacggttcc atgcagaaat
     1021 aaactccttc tccgacattt gaaacgatgc tataaagttt tggtttctct tgataaattc
     1081 cgccttcgtt aactgtcctt ttaacagcat ttcctgatgg ttaaaaaagg attccgattt
     1141 tctaaatgca taccatatag cctttgcccc tacgtaacac gcgccatgtt cagcaataac
     1201 gtcaaaggcc gtatctagat ttacctcaat aaaatcctgc aaattgacaa ttatatagcg
     1261 catagatttt ataacttaaa gtgcgacaaa ataccctcga gctgatccac atcattataa
     1321 cgaatcatca ccataccttg accgtttcgc gcgctatgat ttatttttac tgcagcaccc
     1381 aacttctcag aaagatcttg ttgtaacctt ttgatgttag gatcatccac ggcaggtact
     1441 ttctcactgc gattttcttg cagtttattt actaaacgtt cggtttctcg aaccgataaa
     1501 ttacgagata ccacggtttt tgctacttgc gactggatag gcccttttag tgcgagcaat
     1561 gcgcgtgcat ggcccatgtc taattgtcct tgctctagca ataactgcac atcatcattt
     1621 aacgttaata atcgcagtag atttgttaca ctggtgcggg atttgccaac cgcttccgca
     1681 acttgcaaat gggttaaatt tatttcatcc gctaatcttt ttaaaccacg tgcttcttca
     1741 atcgggttta ggttttcgcg ttgaatattc tcaataagtg ctacagcaaa tgcggtttct
     1801 tcagacattt ctttaatgat aacggggatg gtataaatac ctgccaattg ggctgcacgc
     1861 cagcggcgtt ctcccgccac aatttcgtag tggtcgtcac ttttctttcg caccaaaata
     1921 ggttgcaaaa taccttgcgc acgcatcgat tctgctaact gttcgatacc ttcgctagaa
     1981 attccaagcc gtggttgaaa gggccccggt ttgacctgct cgataggaac atttttaaat
     2041 acctccatgg gagtcttaac tatgctttcc gtatcgtcag aagtttcgcg accttccgct
     2101 agaacactga ccgaaagtag ctcatttagt ccttttccta ggcgactgtt ctttgccatc
     2161 gtaacctcaa atttctaatg cggcctcttt ttggttgagc tctttttttg aattcgccgt
     2221 agtatgtcta cgtagtaact catttcccaa cgttaaataa gcgctcgaac ctctggaatt
     2281 tttttcgtat aaaataatgg gcaacccata actgggcgcc tccgctaacc taatatttct
     2341 tgggattacc gtttgaaaaa ctttattacc aaaatgctca atcagttggt ttgaaacctc
     2401 actagataag cgatttcttc catcatacag agttctaatg atccctccga tataaagcct
     2461 tggattaagt gttgcttgca actggctaat agtgtttaac aaatcgctta atccttccag
     2521 tgcataatat tcgcattgca ttgcaataat aacagcgtct gctgctgtta gggcattaac
     2581 tgttaacata tttaaggatg ggggacaatc aagcagaata aaatcatatt ggtcgcggaa
     2641 aggcgccaga gcatctgcca aaatacgctc ttgtttacct tttttcaata aagcgacttc
     2701 tgctgctgtg agatcacaat ttgcggcgac taaatcatat cccgcaggtg tttgccgaat
     2761 aatcgcctca tccatgctaa cttgttgcaa taacaaatca ttcatagatt tttcaactgc
     2821 atttttatct actccactgc ccatggtagc attcccttgg ggatccatat ctagaagcag
     2881 aacgcgttga ttcaaaacga cgagtgatgc tgccaaattc acacaacttg tggtcttccc
     2941 tacccctcct ttttgatttg caaaggcata gatgcgctgt gccatgatat tgcactctcc
     3001 cataattagg tggtttgctc aaattttata attattaaat gtcgctgtgg tgcttgggaa
     3061 gtattttgca acgttattat acggtcaatc ttaaactttg gatcaatctc ttttaattct
     3121 ttctcctcaa tctttccttt cattgcaaca atcgaaccat ccgaagataa caaatgttgt
     3181 acaagtcgta acatttccgc taaaggttta aaggcgcgag ctactataca agaaaactta
     3241 aaacttgcgt taaaattttc aacgcgggtg caataaagtt gcacattggg caaatgcaac
     3301 tgatgcacca cttgctttag aaaaatttgt cttttttcat tgctatctag caaac
//
LOCUS       DASTIB010000183         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_144174, whole genome shotgun sequence.
ACCESSION   DASTIB010000183 DASTIB010000000
VERSION     DASTIB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144174"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="hutU"
                     /locus_tag="VFP93_03135"
     CDS             <1..898
                     /gene="hutU"
                     /locus_tag="VFP93_03135"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET9843635.1"
                     /translation="DLLTDQTSAHDPLYGYVPKGWDLTHLMEARVREPERVIKEARKS
                     IATHVQAMLAFKQRGVPTFDYGNNIRQIAFEEGVIDAFSFPGFVPAYIRPLFCQGKGP
                     FRWVALSGDPQDIDQTDQCVKSLIPDPFLHQWLDMARIKIPFQGLPARICWLGLGERA
                     KVGLAFNDLVASGKVKAPIVIGRDHLDSGSVASPNRETENMLDGSDSVSDWPILNALL
                     NSVSGATWVSFHHGGGVGMGFSQHAGFVLVCDGTQEAKSRIARVLHNDPGTGVMRHAD
                     AGYLSAIQCAKEHNLDLPMVSL"
     gene            895..2427
                     /gene="hutH"
                     /locus_tag="VFP93_03140"
     CDS             895..2427
                     /gene="hutH"
                     /locus_tag="VFP93_03140"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET9843636.1"
                     /translation="MNTFLLNAGELSLEQLEQIILHNQEIIISEETYQKIQNGFNTLQ
                     SIMATGKKIYGVNTGFGKLAQENISKEDLALLQKKLLLSHATGVGTFFHDSIVALVLL
                     LKINSLSLGFSGIRREIIDALLVLLNNKYYPCVPSQGSVGASGDLAPLAHLSCILIGE
                     GYVRHENKILSAKEALEEVGLKETKLAPKEGLSLINGTQVSTALGVFALLQSKKLFES
                     ALIAGSLSVDAASASDQPFESKIHRVRRLPEQIFVAKILKNLLKDSKIRENHKSCNRI
                     QDPYSLRCQPQVMGGCLTQIRTAARILRYEINAATDNPLVFADTQEILSGGNFHAQPV
                     AFACDNIALALAEIGSLAERRIALLLDASQTGLPSFLVENSGLNSGFMLAHVTAASLV
                     TENKHLAHAVSIESIPTSGNQEDHVSMSTYAARRLHTMVDNLAHILSIEMLIACQGID
                     FRAPLQTSPALLPFYHIIRKISSFLNEDRMLSEEIQRLKNAILQGKFQTSETQIIFDY
                     AS"
     gene            complement(2405..2581)
                     /locus_tag="VFP93_03145"
     CDS             complement(2405..2581)
                     /locus_tag="VFP93_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843637.1"
                     /translation="MFIPNFESNKLELEKNRISSIPDQIRVALIPLLRRNTLEGEQFL
                     ASLNLSQPKTRNQK"
     gene            complement(2658..2861)
                     /locus_tag="VFP93_03150"
     CDS             complement(2658..2861)
                     /locus_tag="VFP93_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843638.1"
                     /translation="MALLTLFDATRICRTLEEKNFFHAVETANLDELSRLLAHGKDYF
                     RLLGISDKIVLNCNEKRSTLKND"
     gene            complement(2942..3196)
                     /locus_tag="VFP93_03155"
     CDS             complement(2942..3196)
                     /locus_tag="VFP93_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843639.1"
                     /translation="MAGYTPMHEQENLDEAAVKQHKQDSSSHPLNQELLKSKGMVNVP
                     DKIVRDTRLDLFYKNIKRAEALTEKATQTLFGTKSKKPHN"
     gene            complement(3266..3481)
                     /locus_tag="VFP93_03160"
     CDS             complement(3266..3481)
                     /locus_tag="VFP93_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843640.1"
                     /translation="MADKNVLDFRAQAEQMMRIIEARLKQGQRGMALEFMILKFKALY
                     EQGVASGRLYEKEGIFPYTAFHDDDRS"
BASE COUNT         1052 a          654 c          710 g         1076 t
ORIGIN      
        1 agacttactt accgatcaaa caagtgcgca cgatcctctc tatggttatg ttcccaaagg
       61 atgggactta acccatttaa tggaagcgcg tgtgcgtgaa ccggagcgtg tgattaaaga
      121 agccagaaaa tccatcgcca cgcatgtaca agctatgttg gcattcaagc aacgaggggt
      181 tcccactttt gattatggga ataatattcg acaaattgct tttgaggaag gtgttataga
      241 tgctttttct tttccaggtt tcgttccagc ctatatacgt ccactgttct gtcaaggtaa
      301 gggacctttt cgctgggtag ctttatccgg tgatccgcaa gatattgatc aaacggatca
      361 atgtgtcaaa tctttaatac cggatccttt tttgcatcaa tggttggata tggcacgaat
      421 caaaatacct tttcaaggat tacccgcgcg aatatgttgg ctaggtttgg gtgagcgggc
      481 gaaagttgga ttagcattta atgacctggt ggcctcaggt aaagtgaaag ctcctattgt
      541 tatcggacgc gatcatctag atagtggatc ggtcgcaagc cctaatcgag aaaccgaaaa
      601 catgttggat gggtccgata gtgtttcaga ttggcctatt ttaaatgctt tgttaaatag
      661 tgtgagtggt gctacctggg taagttttca tcatggtggg ggggtaggta tgggattttc
      721 acagcacgca ggctttgtgc tggtgtgtga tggaactcaa gaagctaaat cccgtattgc
      781 gcgtgtgctc cacaatgatc ctggaaccgg tgtgatgcgc catgcggatg caggatatct
      841 atctgcaata caatgtgcca aagaacataa tttagattta cccatggttt ccttatgaat
      901 acttttctat taaatgcagg cgagctttcg cttgagcaat tagaacaaat aatacttcat
      961 aatcaagaaa ttataataag tgaagaaacc tatcaaaaaa tccaaaatgg atttaataca
     1021 ctgcaatcta ttatggcaac cggtaaaaaa atttacggag ttaacacagg ttttggcaaa
     1081 ttagcacagg agaatatttc caaagaggat cttgcgcttt tacaaaaaaa attgcttttg
     1141 tcgcatgcga cgggagtggg tacatttttc catgatagta ttgtcgcctt agtactctta
     1201 ttaaaaatta acagtttaag tttaggtttt tcggggatac gccgtgaaat tatagacgcg
     1261 ttattagttc ttttaaataa caaatattat ccgtgtgtac catctcaagg tagtgtcgga
     1321 gcatccggcg atttagcacc tttagcacat ctttcctgta ttttaatagg agaaggttac
     1381 gtacgccatg aaaacaaaat actttctgca aaagaagctc ttgaagaagt aggcttaaag
     1441 gaaacaaaac tcgctcccaa agaaggttta agtcttatca atggcacaca agtgtcgaca
     1501 gcactcggtg tgttcgcact tttacaaagt aaaaaattat ttgaaagcgc actgattgct
     1561 ggaagtttgt cggtggatgc agcaagtgca tccgaccagc cctttgaaag caaaatccat
     1621 agggtgagaa gattacctga acaaattttc gtagcgaaaa tcttaaaaaa ccttttaaaa
     1681 gatagtaaaa ttcgtgaaaa tcacaaaagt tgtaatagaa ttcaggatcc ttattccttg
     1741 cgctgtcaac cgcaagttat gggtggttgt ttaacgcaaa ttcgaacggc tgcaagaatt
     1801 ttacgttatg aaatcaatgc agccaccgat aatccgcttg tatttgcgga tacacaagaa
     1861 atattatccg gcggcaattt tcatgcgcaa ccagttgcct ttgcctgtga caatatagct
     1921 ttggctttgg cagaaatcgg ctctttggca gaacgccgta ttgctttatt actcgatgcg
     1981 agccaaaccg gattaccttc gtttctggtt gaaaatagtg ggttgaattc aggatttatg
     2041 ttggcgcatg taacagcggc ttctctggtt acggaaaata aacatttggc gcatgccgta
     2101 agtattgaaa gcattcccac ttccggtaat caagaagacc atgtgagcat gtcaacatac
     2161 gctgcacgcc ggttgcatac gatggttgat aacttagccc atatactgag tatagaaatg
     2221 cttatagctt gccaagggat agattttcgt gctcctttgc aaacttcccc tgccttgctt
     2281 cctttctatc acattattag aaaaataagt tcttttttaa acgaagaccg tatgctttcc
     2341 gaggaaatac aaagactaaa aaatgcaatt ttacagggga agttccaaac ctcagaaaca
     2401 caaattattt ttgattacgc gtcttaggtt gggataaatt aagactagcg aggaattgtt
     2461 caccttccag agtatttcgg cgcaataacg gtattaatgc tacacgtatt tgatcgggaa
     2521 tagaggatat tcgatttttt tccagttcca atttattgct ttcaaaatta gggataaaca
     2581 tgagaaaatc tcaatagttt gcaaattaac caaatgctat cacaaacacc taggtagtaa
     2641 agcatattcg tcgttttcta atcatttttt aacgtagaac gtttttcatt acaatttaag
     2701 acaattttgt cgctgatccc aaggagtcga aaataatctt taccatgagc aagaagtcga
     2761 gataattcgt ctagattagc tgtttcgact gcatgaaaaa aatttttttc ttctagagtt
     2821 cgacaaatgc gagttgcatc aaataatgtt aaaagcgcca tgtttcaaca attgtaaatt
     2881 taagaaacac aagtatgcca caatggatga aaaatccaag atagttattg aagttgagtg
     2941 cctaattatg tggttttttt gacttcgtac cgaatagggt ttgcgtggct ttttctgtta
     3001 acgcttcggc acgcttgata tttttataaa acaaatctaa ccgtgtatca cgaactattt
     3061 tatcgggaac gtttaccatc cctttacttt ttaacagctc ttgatttaaa gggtgagaac
     3121 tagaatcttg cttatgttgt ttaacagcgg cttcatccag gttttcttgc tcatgcatag
     3181 gtgtatatcc tgccatttac aacacataag ttagaagacc ttcttaaaat tatagcaata
     3241 ttgaattatt agatatagag gctatctaag acctatcatc atcgtgaaat gcagtgtatg
     3301 gaaaaatgcc ctccttttca taaagtcgcc cactagctac tccttgttca tataaagctt
     3361 taaactttag gatcataaat tctaaagcca tacccctttg tccttgtttt agtcgcgcct
     3421 ctataattcg catcatttgc tccgcttgag cacgaaaatc taaaacattt ttatcagcca
     3481 tttatccaaa at
//
LOCUS       DASTIB010000184         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1454368, whole genome shotgun sequence.
ACCESSION   DASTIB010000184 DASTIB010000000
VERSION     DASTIB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454368"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFP93_03165"
     CDS             <1..288
                     /locus_tag="VFP93_03165"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HET9843641.1"
                     /translation="RIKYRRAPLCIRSKDSNITYASLILDIDPVRKIILLEEFFSKQG
                     LHVFKPKQTIVVSTRYNGQHVNFECVIQKKMRHHGLQGLVVQWPDKIAYLH"
     gene            514..589
                     /locus_tag="VFP93_03170"
     tRNA            514..589
                     /locus_tag="VFP93_03170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:547..549,aa:Arg,seq:cct)
     gene            complement(628..1815)
                     /locus_tag="VFP93_03175"
                     /pseudo
     CDS             complement(628..1815)
                     /locus_tag="VFP93_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          586 a          419 c          379 g          628 t
ORIGIN      
        1 agaataaaat atcgtcgcgc ccccctttgc attcgatcga aagattctaa tataacgtat
       61 gcaagtttaa ttctcgatat tgatccggtt cgtaaaatta ttttattaga agaatttttt
      121 tcgaagcaag gtttgcatgt gtttaagccg aaacaaacga ttgtggtgag tacgcgctat
      181 aatggtcaac atgttaactt tgaatgcgtt atccaaaaga aaatgcgaca ccatggcctt
      241 cagggcttag ttgtccaatg gcccgataaa attgcctatt tacattaacg aaacaagctg
      301 aatagcttat acttctggtt gccttttttg caaaacctta ttattatgac actcaaaact
      361 ttttgagtgc atttaatgga agaaaatcaa cccaaatcta ttgatttaaa accttaccaa
      421 ccttttggga tccccattat taaattaatg tcaggccttg ttttattttc ccttcttgtg
      481 acagcaattt acgaatacgt tctataaatt tacgcctcgg tggcacagag gatagcgcgg
      541 ccccctccta aggggcaggt cgcaggttcg attcctgccc gaggcgccaa taaaatcaag
      601 gtccgttctt gagacatagt taacagttta tactggagac atgggtaaca cttttgactc
      661 aaaaggattc tcgataggtt ccaccctaca ggattcttca tcaaagtaac ccaagtcata
      721 cttcataaaa gaaacgagcc agattttatc atctttttgt gttattccaa cgttttgccc
      781 agcgagcaca gagcttaaat taattttttt cctacctaaa cagattctgc cgcatttggt
      841 tactgtaatg actttctcat gaaaagggta ttcaagttct ggtaatccaa aatatttctt
      901 agtggaaagt gtatatagct ctcctgggta cctcatatta attgcttgat ggggacgttg
      961 attattgtat acctcttgaa agtcatcgaa tctctgttgc tgttgtaaaa aattaaattc
     1021 cgatggtttt gttgcctctt tctttaaggt taaatgcatt cgttcatggc gcccattttc
     1081 ctgaggattc cctggccgaa tacgttcaat cgaaatacct aagcgtaacc accacactga
     1141 aagccggctt agcccataca acgcattggg agaggcaaaa ggtactccat tatctgtgcg
     1201 tatagcatta gaatgacata ttccttgaat gttctttcaa aaatagtaaa agcaaattgc
     1261 tcttttgttg attccaatgc ttcgcaggtt aggagaaaac gcgagtgata atcggtaatc
     1321 gttagagggt aacaatatcg cttattttcc tagcaagaat tcccctttat aatcagcgca
     1381 ccatagctga ttaggcttct ttgcatggga taactctgtt ccttcagctt tatagcgcct
     1441 gcgacgcgga cgggtaacta agccatgacg gtcgagcacc gcgtgtatgg tgcttgttgc
     1501 tggcagttga atatcgggat attggcgtaa taatttttct cttagttttg gtgcgcccca
     1561 agtagggtat tcttttttga tttgaagaat agctctttca atctgaaatg gtagctgatt
     1621 tgcatatcta ttgggccgtc gagtacgatc agataatcct tcaagaccac actctttata
     1681 tcgactgaat attttatatc ccgttttccg agaaattcca aattccttac acagaggaga
     1741 cattttttca ccttcaagaa ggcgagcaac gaactttaaa cgctcatcca caacacaact
     1801 ctccttccaa gacatagaaa aacacctctg aaaaaccaaa agtgtaacct atgtcttcgg
     1861 aatattgtgt taactatctc tcaggaagga cacaaggggc ctcgcccgtc tgcctaaggt
     1921 ggattctaga aaaaatttgt ttaagtataa ccacggtata aacaaatcta ttctaactac
     1981 tactttagtc gaaaccaacc taacgctgaa ta
//
LOCUS       DASTIB010000185         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_445392, whole genome shotgun sequence.
ACCESSION   DASTIB010000185 DASTIB010000000
VERSION     DASTIB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445392"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VFP93_03180"
     CDS             <1..970
                     /locus_tag="VFP93_03180"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET9843642.1"
                     /translation="PTAILVSASLLVHKQLLPALDHLHKTLANKSEQLHTITKTGRTH
                     LMDAMPVTFGQEIGGWAEQIKLGIDRITSTLPRIHALPIGGTAVGTGINAPENYGTLV
                     CAELSQNIGIDFISNPNRFEGLSAQDTAVELSGQLKVIATSLMKISNDLRWMNSGPLA
                     GLGEIILPALQPGSSIMPGKVNPVIPEAVAMAAAQVIGNDSTITIAGMSGNFQLNVML
                     PVIGYNLIQSIEILSNGALVLADKAIQGFQVNEKQIQNTLEKNPILVTALNSVIGYEL
                     GANIAKIAYKENRPILDVAKELTDLSEGELQRLLDPNILTQGGIPK"
     gene            complement(994..>3142)
                     /locus_tag="VFP93_03185"
     CDS             complement(994..>3142)
                     /locus_tag="VFP93_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843643.1"
                     /translation="TLFDNMTPLFHAIKLKRSLFARHLIRANVNLNAVCHEVSILHQA
                     ILSKEEVIIQELLQKGVDKSVPVQALTALSSVAIFDDLFPHATLYGYKFSSLLKDPSL
                     ISSISTFQMFRRNTTILGYLEKNNFCTELVIAALRILELADKIKEFERSQIVGNETAM
                     NDAKVLRARWHFNNNVQPHFKDKFLQYSSAKDADEALIAVEKHIKAVILEEIMKRAQV
                     ENNTTIVDFLTTHGNALIAGQEDALKASCSVLNAMDTLQSAWRCYNPYAPVKANWLNL
                     LTEPVNDIEVFSIAAAHNGQITAKQASKIIRERVAYYFLAVMDENDGDESVRETRKMN
                     FFSQLQDIRNAHGPDDPTCYPGAITRCADMGSFHDVAQLPTTTKEWLSDYFKTKALAT
                     LAQEFTKLTTFEEKEALSSAIIQLTEGSINAIIQNPTNYPKEWLALRKHFIHSLGTSQ
                     ENLEKILLEGTSPITQADLIYIEQCLLDIGAGDRGQAFATFFNSNTDYIPSQEEIEQA
                     NPFKSHENAYALSKIMLDIILHLVPSYTKSMRQLKTLTEFLQQKVPALLQGQPLSEII
                     DDMRLTPETQAAVFQAIDANWKFAPAVVNPFTAKIEAARKQIELKANQPQFQMVFKRQ
                     LDNLLRKSELFLKHYPMIKEIVENANPKLDATDAIRIAEELANYFALEETFIQIEFQK
                     QFSEAYVAKNAALFEMLSAKLTNPTGQTPRTSL"
BASE COUNT          942 a          615 c          604 g          981 t
ORIGIN      
        1 tccaacggct attctagtta gcgcaagtct tttagtgcat aagcaattat tacctgcttt
       61 agatcacttg cacaaaacgt tagcaaataa atctgaacaa cttcatacca taaccaaaac
      121 gggacgcacg catctgatgg atgccatgcc tgtgactttc ggccaagaaa taggtggctg
      181 ggcagaacaa ataaaacttg gaatagaccg cataaccagc accctaccaa gaattcatgc
      241 actacccatc ggtggtactg cggtcggaac aggaatcaat gctcctgaaa attatggcac
      301 tttagtatgt gcagaattat cccaaaatat aggtatagac tttatcagca acccaaatcg
      361 ctttgaaggg ttgagtgctc aggacacggc tgttgaatta tcgggacagc ttaaggtaat
      421 tgctactagt ttaatgaaaa tatcgaatga cttacgctgg atgaattccg gtcctttagc
      481 aggcttggga gaaattattc tacctgcttt gcaacctggc agcagtataa tgccgggaaa
      541 ggtaaatcct gtgatccctg aagctgttgc aatggcagct gctcaagtga taggaaacga
      601 tagcacgatt acaattgcag gaatgtctgg taactttcaa ctcaatgtaa tgttaccggt
      661 tataggttat aacttaatac aaagcatcga aattttgagt aatggcgccc tagtccttgc
      721 cgataaagcc attcaaggtt ttcaagtcaa tgaaaagcaa attcaaaaca ctttggagaa
      781 aaacccaatt ttggtgactg cactcaatag cgttattggg tatgaattag gtgctaacat
      841 tgcaaaaatt gcgtataaag agaatcgccc tattctagac gtagctaaag agcttaccga
      901 tctttccgag ggcgaattac aaagattatt agaccccaat attctaacgc aaggagggat
      961 cccaaaataa tccccttagc gctagtgaca catttaaagc gaagtacgcg gcgtttgacc
     1021 tgttggattc gtcaacttag cggatagcat ctcaaacaac gcggcatttt tagcaacgta
     1081 cgcttcacta aattgctttt gaaattcaat ctgaataaag gtttcttcaa gcgcaaaata
     1141 attcgctaac tcttccgcaa tacgaatcgc gtccgtagca tctaattttg gattcgcgtt
     1201 ttcaacaatt tcttttatca tcggataatg tttaaggaat agttcgcttt ttcgcaataa
     1261 gttatctaat tggcgcttaa ataccatttg aaattggggt tgatttgctt ttaattctat
     1321 ttgttttcta gcagcttcaa ttttcgccgt aaaaggatta actactgctg gtgcaaattt
     1381 ccaattagca tcaatagcct gaaaaaccgc agcctgagtc tcaggtgtaa gacgcatatc
     1441 atctatgatc tcacttaacg gttgcccctg cagtaaagca ggtacttttt gttgcaagaa
     1501 ttctgttagt gtctttaatt gacgcatact ttttgtataa cttgggacca aatgtaaaat
     1561 aatatctaac atgattttac tgagtgcata tgcgttttcg tgcgacttaa atggattggc
     1621 ttgctcgatc tcttcctggc ttggaatata atcggtattg ctattaaaaa atgtagcaaa
     1681 ggcttgacct ctatcacctg cgccaatatc caataaacat tgttcaatat atattaaatc
     1741 ggcctgggta attggagatg taccctctaa caatattttt tccaagtttt cttgcgaagt
     1801 tcccaaggaa tgaataaagt gctttcttaa tgctaaccat tcttttggat aattcgttgg
     1861 attttgaatg attgcattaa tagaaccttc tgttaattgt attattgccg agcttaaagc
     1921 ttctttttct tcaaatgtag ttaacttagt aaattcttgt gcgagagttg ctaacgcttt
     1981 ggttttaaaa tagtctgaca accattcttt tgttgtggtt ggtaattgtg cgacatcatg
     2041 aaaagaaccc atatcggcac agcgcgtaat agcaccggga taacacgtgg gatcatcagg
     2101 gccatgcgcg tttcgaatat cttgcagctg gctaaaaaaa ttcatttttc tcgtttctcg
     2161 tacactctca tcaccatcat tttcatccat gactgctaag aagtaataag caacgcgctc
     2221 tcgaataatt ttagaagctt gttttgcagt gatctgccca ttgtgggcag cagcaatcga
     2281 aaagacttca atatcattta caggttcagt cagtaaattt agccaattcg cttttactgg
     2341 ggcataagga ttgtaacagc gccaagcgga ttgtaaagta tccattgcgt ttaaaaccga
     2401 acaagaagct ttcaacgcat cttcttgtcc tgctattaaa gcatttccat gcgtggttaa
     2461 gaaatctaca atagttgtgt tattctcaac ttgagctctc ttcattattt cttctaaaat
     2521 aacagccttt atgtgctttt cgactgcaat gagcgcttcg tctgcgtctt ttgcactcga
     2581 atattgcaaa aatttatctt taaaatgggg ttggacgttg ttattgaagt gccaacgtgc
     2641 gcgcaatact ttagcatcat tcatcgcagt ttcattgcca acaatttggg aacgttcaaa
     2701 ttcttttatt ttgtcagcta attccaaaat tcgtaatgcc gctatcacta attcagtgca
     2761 aaaattattt ttttctaaat aaccaagtat cgtggtattc ctgcgaaaca tctgaaaggt
     2821 ggatatagat gaaattaaac tcgggtcttt taacaaactg gaaaacttat agccatataa
     2881 tgtagcatgg ggaaataaat catcgaaaat ggccacactg gataatgctg tgagtgcttg
     2941 cacaggcaca gacttatcaa cgcccttttg taacagttcc tgaataatca cttcctcttt
     3001 acttaatatt gcttgatgca gaatagaaac ttcatgacaa actgcattca aattgacgtt
     3061 cgctcgaatt aaatgtcgag caaataagct acgtttgagt ttaatcgcat gaaataaggg
     3121 tgtcatattg tcaaagagtg tt
//
LOCUS       DASTIB010000186         4786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_358640, whole genome shotgun sequence.
ACCESSION   DASTIB010000186 DASTIB010000000
VERSION     DASTIB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358640"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2300)
                     /locus_tag="VFP93_03190"
     CDS             complement(<1..2300)
                     /locus_tag="VFP93_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843644.1"
                     /translation="MDKRFEGNIPATNKFNEHRKNERADAVDMVSKLLATFQDNKEAK
                     AYILQIVQNIQKRLNLIEQNTARIIDYPIHKEAQKYGKYYNQDCYLQLMPYCKLAHLS
                     EKNGIPEEHALKLSIMFDEPLSALKYIQDNISQNNLAIHDACLLTLPDVNQCNFSEWK
                     KLIKTNINDGTFRALLPKAPELETILEKNATAAKPNIKAISEKKKEIEKVNKKFKQLD
                     RKHGDLEDDEKEEHKQLLQQLAALKLELFAMSAGIKLHEADLSILAACDEYYLQQTSG
                     AYKYFLQHGLTKDDYAKFLKLERKDDDAQIPNIKLDGNDIGHPDTYLMKVPVLNEMWA
                     AKAACLGKESNCCQSLSGEAGEVCVIYGLTSPYSGFYVLCKGDVNNPKVDDPLLGQCW
                     AWRSKTGAIVFDSIETKSTEENTQNMVAAFFEKLAETLVLENHTNKVACGPTSGISSK
                     VGILSILNETEQFIDYAEYSDSSVLQRVLRDKINPFYLYGKDDASTQETEESIENVMK
                     IDCPLVHSTFLCSLLNHLATQRGMGKNDMEMAMNYYRSEDNHDTTWGSFELEKIREIA
                     TKYNRTQEFNTIVETLNLYLGGKLSENEILSAMVNRSFYTSIKDSVGKTTLMYAIKKG
                     YVDLACKLIDEGININEKDKIFGYSAFTMAAMEGNTKVALKLIENGVIVNEKSNGGAT
                     ALMRASTPDFVRMLLERGAQVNEQDVAGYTPLMCAINLCNIDTATILLENGADIHAKN
                     FNGETALDLLAKTNPYYLSALQEKIN"
     gene            complement(2903..3094)
                     /locus_tag="VFP93_03195"
     CDS             complement(2903..3094)
                     /locus_tag="VFP93_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843645.1"
                     /translation="MIQITNVELSIVSGGKNCLVDFRVAHGITDPNVVCPAVMVPVDH
                     QGKPLPIETFRLEDLINIK"
     gene            3512..>4786
                     /locus_tag="VFP93_03200"
     CDS             3512..>4786
                     /locus_tag="VFP93_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843646.1"
                     /translation="MLFSAYLNTFIKDEVVEIGYGKQMELDQLIQFIESAPLDEILKK
                     IDFNHYSIKHESTLLSYTAQFYFPLLTVILRRVPEKYLDFSKLTHEALIEIICKLCLR
                     DDVVLIKKFKNKLDLNAICSPIKGATVFFIIIEILYEFPHAMLIFLENTSLDEVNFKK
                     NAYDAPLVLIIKSALKYNFPSLLNILLEKIQFRDLEFTDGIFDFNHLDCKYRYGNVVE
                     WLATTSYAHYVDKFLDLGIKQGEEIFHHLLANKNHNENVNVAQIPIFEQQIKKLEDYI
                     KLSSAPAHALKLAQFYERLGLHETTKEFVKRLCFDHPYRAAIYDLLAQSMVYNFAETS
                     HSEPNNYKSKLLKAVKYSLEGSPALRKQLSLTFIYQGKACSDVFPSQTKEPFFNSLAD
                     LKAPIAKEEKKLILNALSLIREREELQTKPFDN"
BASE COUNT         1476 a          854 c          793 g         1663 t
ORIGIN      
        1 tcgtttattt tttcctgcag ggcactcaaa tagtagggat tagtttttgc tagcagatct
       61 aaagcggttt caccattaaa gttctttgca tgtatatccg cgccattttc aagtagaata
      121 gtggcagtgt ctatattgca aagatttata gcgcacatta agggggtata gccagccaca
      181 tcctgttcat tcacttgtgc ccctctttca agtaacatgc gcacaaagtc tggagtgctt
      241 gctctcatca aagcggtagc gccaccgttt gatttttcat ttacaattac tccattttca
      301 atcaacttga gcgcaacttt ggtattccct tccatagctg ccatggtgaa agcagaatag
      361 ccaaaaattt tatctttttc atttatattg attccttcgt caattagctt gcatgcaaga
      421 tcaacataac ctttttttat cgcatacatt aatgtggttt ttccaacgct atcctttatg
      481 gaagtataaa atgagcgatt taccattgcg gataaaattt cattttctga taacttcccc
      541 cccagataca gattcaaagt ctctactata gtattaaatt cttgggttcg attatatttc
      601 gttgcaattt ctcttatttt ttcaagttca aagctacccc atgtcgtgtc atggttgtct
      661 tcgctgcgat aataattcat tgccatttcc atatcatttt ttcccatacc acgctgggtc
      721 gctagatggt ttaataacga acataagaat gttgagtgga ctaagggaca atctattttc
      781 atcacatttt ctatggattc ttctgtttcc tgggtgcttg catcgtcttt gccgtacaaa
      841 taaaacgggt tgattttatc gcgtaacact ctctgtagta ctgatgaatc ggagtattct
      901 gcgtagtcta taaattgctc tgtttcattt agtatagaga gtatccctac tttactcgag
      961 atccccgaag ttggtccaca ggctacttta ttcgtatgat tctctaatac aagtgtttca
     1021 gccaattttt caaaaaaggc agctaccata ttttgcgtgt tttcttcggt acttttggtc
     1081 tctattgaat cgaatacgat tgcaccagtt ttactgcgcc aagcccaaca ttggcctaat
     1141 aagggatcgt caactttagg attatttaca tcccccttgc aaagtacata aaatccactg
     1201 tacggacttg taagtccata tattacgcaa acctctcccg cttcaccaga aagagattga
     1261 caacaattgg attcttttcc taaacaggcg gctttggctg cccacatttc gttcaataca
     1321 ggcactttca ttaagtaagt gtctgggtga ccaatgtcgt taccatccag tttgatgttg
     1381 ggaatttgcg catcgtcatc cttgcgctca agttttaaaa atttagcgta gtcatcttta
     1441 gttaagccat gttgcagaaa atacttatag gcacccgaag tttgttgcag ataatattca
     1501 tcgcatgcag ccaaaataga taaatctgct tcatgtagct ttatgcctgc actcatcgca
     1561 aaaagctcta atttaagtgc agctaattgt tgcagcaatt gcttgtgttc ttccttttca
     1621 tcatcttcta ggtcgccatg ttttctgtct aattgtttaa attttttatt tactttttca
     1681 atttcctttt tcttttcgct aatggctttt atgttaggtt tggctgcagt tgcatttttt
     1741 tctaaaatgg tttctagttc tggcgcttta ggtaataatg cgcgaaaagt gccatcgttt
     1801 atatttgttt tgataagttt tttccattca gaaaaattgc attgattgac atcaggtaaa
     1861 gtcaacaaac aagcgtcatg tatggctaag ttattttggg atatattgtc ttgaatatat
     1921 tttaaagcag acaatggttc atcgaacatt atagaaagct ttaatgcatg ctcttcagga
     1981 attccatttt tttcacttag atgtgctaat ttgcaatagg gcataagttg caggtagcaa
     2041 tcctggttgt aatacttgcc atatttttgc gcttccttat gtattgggta gtcaatgatt
     2101 cgagctgtgt tttgttctat aagatttaga cgtttttgaa tattttgtac aatttgcagt
     2161 atgtacgctt tagcttcctt gttatcttga aaggttgcaa gcaactttga aaccatgtca
     2221 accgcatcag cgcgttcatt tttacggtgt tcattaaatt tattcgtagc aggaatatta
     2281 ccttcgaacc ttttatccat gactttgcag ctgtcacaaa ataatttatc gattgtattt
     2341 agagtcttaa gtatatcttt gaaatccatt gaaatccccg gttttagcag tacagcgaca
     2401 ttgtatggtg tgtaagctag tataaagttt ttgagaatgt gcctaaatct caatcagatg
     2461 agaggtaaat tttgctagag taatggttat ccttaaacac tcagatagct ttatgttaaa
     2521 aactgcatat gtagtcacta attggtatat aaccgttggc tttaaaattg gaaattaatc
     2581 gcagatcagc aggtgttatt ggcagatcaa tccacggaat taaaaaactc catagcattg
     2641 aaccaaattg actgtgagaa gaacaaaaag cgctcgggat attattatta attttgtata
     2701 aagcctacaa cacctacttg gcatgattta tttaagccat ccataaaatt tctaacgata
     2761 ggattgcctg aattaacatt aataagagag taggaaattt caagcatgga tattgcatca
     2821 gagtagtaat cacgatttga tgacagtacc cattccaagc aaaatcctgc ttgtgtaaaa
     2881 aacggtttag gatataccaa tatcacttga tatttatcaa atcttccaaa cgaaaagttt
     2941 caataggaag tggtttccct tggtgatcaa ctggcaccat taccgcagga caaactacgt
     3001 ttggatctgt aattccatga gcaacccgaa aatcgactaa gcaatttttc ccgccactga
     3061 caatggataa ttccacattc gtaatttgaa tcataatcat ttctcctaga tagttaaata
     3121 atcagagaga ttatatataa gtcagataac atgtcaaata taaaattgcc gtttgtccat
     3181 taagaacgac gatttgactc tttaaaggcc gtttttatcg tatctcatta agcagatcat
     3241 tatataggtt taaacttaaa cgatgcacat tctcatataa aaattcagta atactgaaat
     3301 tcagcgtcat ttcctttatt tttataaata cttcgttatc aggatttttt tttaattcgt
     3361 ttattaagtt tcttatgctt atcactggta tttgagagtt tgattaaaga taaaaaaggt
     3421 tttacactaa gttcgttata tcctgttcta taaagagatt taacttatat gagttcagaa
     3481 attaacacaa ctttttttaa gaataaatag tatgttattt tccgcatacc taaatacatt
     3541 tatcaaagat gaagtggtag aaataggcta tggaaaacaa atggaattgg atcaattaat
     3601 tcaatttatt gaaagtgcgc cacttgatga aatattaaaa aagatcgatt tcaatcatta
     3661 ttcgattaag catgaatcaa cactgcttag ttacactgcg caattttact tcccactttt
     3721 gacagtaatt ttacgtcgtg taccagaaaa gtatttagat ttttcaaaat taacgcatga
     3781 agcgcttatt gaaataattt gtaaactttg cttgcgggat gacgttgttt taataaaaaa
     3841 atttaaaaac aaacttgatc tcaatgcaat ttgctctcca attaaaggag caaccgtatt
     3901 tttcattatt atagaaattt tatatgaatt tccccatgca atgttaatat ttctagaaaa
     3961 tacatcactc gatgaggtca actttaagaa aaatgcttac gatgccccac tcgttttaat
     4021 tataaaaagt gcattgaaat ataattttcc ttcgctttta aatattttgt tagagaaaat
     4081 tcaatttagg gatttagagt tcaccgatgg tatttttgac tttaatcatc ttgattgtaa
     4141 atatcgctat ggaaatgtcg tagaatggct tgctactacc tcttatgctc attacgtaga
     4201 taaatttctg gatttaggca taaaacaagg tgaagaaatt tttcatcact tgctcgcaaa
     4261 taaaaatcat aatgaaaatg ttaacgttgc acaaatacct atttttgaac agcaaataaa
     4321 aaaattagaa gattatataa aattatcaag tgctccagcc catgccctca agttagcaca
     4381 attttatgaa cgattaggtc ttcacgaaac aacgaaagaa tttgtaaaaa ggctttgctt
     4441 tgaccaccct tatcgagccg caatttatga tctgttagcg caatcaatgg tttataattt
     4501 cgcagaaact tcgcatagtg aacctaataa ttataaaagc aagcttttaa aagcagtaaa
     4561 atattctctt gagggtagtc ctgcacttcg aaaacaactt agtcttacat ttatctatca
     4621 gggcaaagca tgttcagatg tttttccttc acaaactaaa gaaccttttt ttaattcttt
     4681 agcagaccta aaagcaccaa tcgcaaaaga agaaaaaaag ttgatactca atgctttaag
     4741 cttaatacga gaaagagaag agctccaaac aaaacctttt gataat
//
LOCUS       DASTIB010000187         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1396484, whole genome shotgun sequence.
ACCESSION   DASTIB010000187 DASTIB010000000
VERSION     DASTIB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396484"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFP93_03205"
     CDS             complement(<1..217)
                     /locus_tag="VFP93_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843647.1"
                     /translation="MFDGPKKTSRLDLEQAISNENLTNTTHEVIPPTRIDMFLEREVE
                     NKYLYKMCDLGDNPKYEVVSGHKVPAKK"
     gene            617..>2111
                     /locus_tag="VFP93_03210"
     CDS             617..>2111
                     /locus_tag="VFP93_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9843648.1"
                     /translation="MLSLKLNLFPSPISRWIDNVISNSLATEPHWIIRAIYARAVACT
                     IFPLSLINNIVYLGFLLVKSFIKKMCGLQKTPTNYKEIFYHLGATIIGFIAFPLGLIA
                     PDIVSYHFLEVQTNRASTDFGKLDHIHGKHVFPKSIQEVIDILQDAHNNHKTVAVSGN
                     QFSQGGQTLPQEDGIFLHMSSFKEITLSPDKNVATVGAGVTWEELQKAIDPFKKAVQV
                     MQASNIFTVGGSLAANVHGWDHRHGSLAETVESITFIDGKGVLHKDITRNSPLFKLML
                     GGYGTLGVIVEAKIKLAKNHVLKKKGKILSLEEYIQTFANTIKNNKEIKLHYGRIALG
                     PNENFDKILMVNYYKTNKPVNILQSLPKETNSPFERICLHILRRLPFLKKIKQWYDNR
                     KYTKKEILTRNQAMQPKIKYITPHASNHDLDLLQEYFIPKNHLKKFLSDLNVLVKNNN
                     LNIINATIRYVKKDFISIMPYANEDCFSIVLYLNSKRDADTLQKVKKV"
BASE COUNT          746 a          348 c          345 g          672 t
ORIGIN      
        1 ccttttttgc aggcactttg tgtccagata ctacttcata ttttgggtta tcgcctaaat
       61 cgcacatttt atataaatat ttattttcta cctcacgctc caaaaacatg tcaatccgcg
      121 taggtgggat tacttcgtgg gtagtgtttg ttaaattctc attagatata gcttgttcta
      181 aatcaagtct gctagttttt tttggtccgt caaacatttt gctctcctaa aatgtatttt
      241 catatacgat ttatcaggaa ataaaaagat gacataaatt aatctttgtg tcacttttta
      301 cgatgacgca atacagaaga caaaaaagac aaaccggact cacaggcaga ctgaaataaa
      361 gtacgcccct ataaaaatac tctgaaaatt agatcatttt tcaaaagcaa tttcaaacat
      421 aaaaaaaaat gctgtttaag gtttgtgttc tagtgatata aaattaaatt tttttatacg
      481 gcaatcttta ccttctcaga acaaaatgca tttttaatat agccttcgga tggataagaa
      541 agctttgcta aaatttgaca cgctttgcac ttttttatat tattcacatc atttgtaaat
      601 agttgctcac attaatatgc ttagcttaaa acttaatctt tttcccagtc caatttcacg
      661 ttggatagat aatgtaataa gcaatagctt agccacagaa ccgcattgga taataagagc
      721 tatatacgca cgagctgtag catgtaccat ttttccactt agcttaataa ataatattgt
      781 ctatttagga tttcttctcg ttaagtcatt tatcaaaaaa atgtgtggtc tacaaaaaac
      841 tccgaccaat tacaaagaga ttttttatca tctgggagcc acaataatag gttttattgc
      901 gtttccttta gggcttattg caccggatat tgtttcttat cattttctag aagtacaaac
      961 aaatcgcgcc tcaacggatt ttggaaagtt agatcatatc catggtaagc atgtattccc
     1021 aaaatcgatt caagaagtga tagatatctt gcaagatgcg cataataacc ataagacggt
     1081 agcagttagt ggtaatcaat tttcgcaggg tggtcaaact ctgcctcaag aagatggaat
     1141 ttttttacat atgtcttctt tcaaggaaat aactctttct ccagacaaaa atgtggctac
     1201 cgtgggtgca ggagtaacct gggaagaatt gcaaaaagca atcgatcctt ttaaaaaagc
     1261 agttcaagta atgcaggctt ccaatatttt caccgtagga ggatctttag cggcaaacgt
     1321 gcatgggtgg gatcatcgtc atggctctct agcagaaacg gtagaatcga taacatttat
     1381 cgatggcaaa ggggtattgc ataaggatat cacaagaaat tctccattat ttaaattaat
     1441 gctcggtgga tatgggactc tgggcgtcat cgtggaagca aagattaaac ttgctaaaaa
     1501 tcatgtctta aaaaagaaag gaaagattct aagtttagaa gaatatatcc aaacctttgc
     1561 gaatacgatt aaaaataaca aagaaattaa attgcattat ggtcgcattg cgttaggtcc
     1621 aaatgaaaat tttgataaaa ttttaatggt aaactattat aaaacgaata agccggtaaa
     1681 tatcttgcaa agtctaccga aagaaactaa ttccccattt gaacgcatat gtttgcacat
     1741 tttaagaagg ttaccatttt tgaaaaaaat aaaacaatgg tatgacaacc gaaaatatac
     1801 aaaaaaagaa attttaacta gaaatcaggc aatgcaacca aaaattaaat atattacccc
     1861 tcacgcctcg aatcatgatc tagatttact tcaagaatat tttattccta aaaatcattt
     1921 aaaaaaattt ttaagtgact taaatgtgct tgttaaaaat aataatttaa acataatcaa
     1981 tgcaaccatt cgttatgtta aaaaagattt tataagtatc atgccctatg ctaacgaaga
     2041 ttgtttttcc attgttttgt acttgaatag caaaagagat gctgacacgc tgcaaaaggt
     2101 taaaaaagta a
//
LOCUS       DASTIB010000188         5663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1409532, whole genome shotgun sequence.
ACCESSION   DASTIB010000188 DASTIB010000000
VERSION     DASTIB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409532"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFP93_03215"
     CDS             <1..527
                     /locus_tag="VFP93_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9843649.1"
                     /translation="APDFALSDVHGENQNLQKQLGKIVVLEWTNPECPFVQKHYNSNN
                     MQDLQQKYTNLGIVWITINSSAQGQSGYMTVEQAHDFAKQYNASPSTLLLDPQGVVGK
                     LYGAKATPHMFIIDKKGVLVYQGAIDSIRSTHADDVKKATNYVDQALTELLNNKPIRI
                     PETKPYGCSVKYKA"
     gene            548..817
                     /locus_tag="VFP93_03220"
     CDS             548..817
                     /locus_tag="VFP93_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843650.1"
                     /translation="MNPAIFRISAIFLMLGFYTQGFCQDSKAIPTKITESKSHVKKNV
                     KNSHTQNNQKNSQLLADELALDSSFTDPEILLELSLREAELMDSR"
     gene            973..1704
                     /locus_tag="VFP93_03225"
     CDS             973..1704
                     /locus_tag="VFP93_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843651.1"
                     /translation="MHFLTFLVLSILQVLCLIHAMKRGYNFSYILAILMFPLVGSIVH
                     FYLSIVPACIHAVKHLISPWKGQPPLKDKVLMYRKLVEKVPSFENKYQLIVALLATQN
                     AFEALEHIQLIQVGMHKNCPNLLQCKAKAQLLLNQKQAAFETVKALMKIDPLQKNKAI
                     CILFAQILEANGHYEAALGEYEKALENRYCFESHYYYIRLLKRLNYKHRAKDEATKLV
                     IRYKKLKHTQKRQLRKWVILTHQLN"
     gene            complement(1701..2294)
                     /locus_tag="VFP93_03230"
     CDS             complement(1701..2294)
                     /locus_tag="VFP93_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843652.1"
                     /translation="MLNYLWSFYRTQQKPEISPISINGDGSIMIPSSVQVHVAPLEMH
                     VLNSSEQSLISSELEKNRQKKFPVNQPGKWITDADWIEVQHVLRSNIFKTTIPTAYKI
                     IIKFIEQEGNYPIFQYYPSTRGLREKQRGCISFSSNKIWVRWDHISHDRWTNSHSLRD
                     FSDKEEFLVWIKTLENVTCVLSDNALWPQLPTRFEYF"
     gene            complement(2304..2762)
                     /locus_tag="VFP93_03235"
     CDS             complement(2304..2762)
                     /locus_tag="VFP93_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9843653.1"
                     /translation="MASQRLKNYLDNHNVYYEVISHPIAFSALQLCEVSHIPSKKMAK
                     TVICKTTNDLFMVVISAHDQVNLEELKKLIGTPVVLATEEEFSGAFPDCEVGAMPPFG
                     NLYDMDVYVSEDLVHGSEIAFNAGNHSELYKMQYKDYEKLVHPKIIHTQH"
     gene            complement(2868..3353)
                     /locus_tag="VFP93_03240"
     CDS             complement(2868..3353)
                     /locus_tag="VFP93_03240"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HET9843654.1"
                     /translation="MRFKKILVCALTLSLVCVSGCQQTRGNNTTTGALIGGATGALAA
                     SHIGKGSGKIAATMLGAFAGAVIGSEVGQYLDEIDREKADDAFHQAANAPVGRTIVWK
                     NPNSGHHGKIVHLREGVASGGEYCREYQSEVTIGNKVEEAYGTACRRPDGSWEILNQK
                     T"
     gene            complement(3435..>5663)
                     /locus_tag="VFP93_03245"
     CDS             complement(3435..>5663)
                     /locus_tag="VFP93_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843655.1"
                     /translation="GILNNFPNAVFDSDKFCEISYPLQNIQYKDYAESVMINPSILGF
                     SIVSKITQLDENITPYSERVQKIAQNSFDKKESVRPQPYWTFSDYIRILLRYKYGKLQ
                     PEDSYKKNKTHPDNTVQIYIENIKTTEFHLDDKKFNLLIHSGFNAARLWHEKFRNPKD
                     FYSGTFHSVSKNLKLTTALDKKEAFYLDKLTHYFSEWMEELTKFKHLPEHELKHCISV
                     QYTSFVVRQFFQKILNKFDENEGYEIITSAWYKSADQAEFKNQALKNFRSSINFFIYP
                     NLLLSKLKQLLEDESSCELFQTLVQGHPQHVLNFIQNDENSLLNLVITKKIKLLPFLF
                     GQIKQCCEQLASSPCANSYFNIVNALPKYPLLFEANVSNCEAIQICIDAGCDPCYKQK
                     NGKTAIHHAIDIGNLSAFLLYVEYIRNYKIDLHTIRYGFHQIPLWHYIVDSPNGSILN
                     SLLEEKHYSSLQYLYVNTQSDNAGVSFLEKLAQKGVYAWRVGMDNFSNSSQLTFTEIK
                     KHVVIKKSHYFEGLAKLRRNILESDDIEWRLKKLRMQDLLALMDPVFLTEQDENFRLN
                     IKIFYTIAQMLLREAGFQNSINLLELTSNGQNIDCNIAVSQPFLKAFPIQKSLSYIAQ
                     WGSAEDFYKKIIKYRANLIDIKQITSTPESASEKSPFHFLIDHKRLDIFKFLKERFEK
                     QERKLYFDDLFDLSSERNLWDYAQEQAPELACYLLEYTRNEYFNQFKIVELTTRVNHL
                     KL"
BASE COUNT         1814 a         1025 c          904 g         1920 t
ORIGIN      
        1 ctgcacctga ttttgcccta tccgatgtgc acggagaaaa tcaaaatttg caaaaacaac
       61 ttggtaaaat tgttgtgctt gaatggacca acccagaatg tccttttgtt caaaaacact
      121 ataattctaa caatatgcaa gatcttcaac aaaaatatac caatttgggt atagtttgga
      181 taactatcaa ttcttctgca caaggtcaat caggttatat gactgtagaa caagcacacg
      241 atttcgcaaa acaatataat gcctcacctt ccacactgtt gttagatccg caaggtgtcg
      301 tcggtaaatt atatggtgcc aaagctacac ctcatatgtt tattattgat aaaaaaggcg
      361 ttttagttta tcaaggcgct attgattcca tccgctccac acatgcggat gatgttaaaa
      421 aagcaacaaa ttatgtagac caagcgctta cagaactatt aaataataaa ccgatacgga
      481 tacccgaaac aaagccctat ggctgctctg taaagtataa agcgtaattt cacaaggaga
      541 caattttatg aacccagcta tttttagaat aagtgcaatt tttcttatgt tagggtttta
      601 tactcagggg ttttgtcagg attcaaaagc aatcccaaca aaaataaccg aaagcaaatc
      661 tcatgttaaa aagaatgtta aaaatagcca cacacaaaat aatcaaaaaa actcacaatt
      721 gttggcagat gaattagcac tagattccag ctttaccgat cctgaaattt tgttagaatt
      781 aagtttaaga gaagcggaac taatggattc acgttaaaac atcaatgttt gaattttaat
      841 ttaaaagatt ttacgaatgc ttatcgtata aaaactacgt tttgtcggtt tatgttcacc
      901 atgatttgtg accttacgct tattttgtta agctgatgag cacacccgca agggtaaaga
      961 catgggggcc ttatgcattt tttaactttt ttagttttaa gcattttaca ggttttgtgc
     1021 ttaatccatg caatgaaacg cggatataat ttctcttata ttttagccat tttaatgttt
     1081 cccttagtag gtagtattgt acatttttat ttaagcatcg tgcctgcttg tatccacgca
     1141 gtaaaacatt taatttcccc ttggaaaggt caaccacctc taaaagataa agtgttaatg
     1201 taccgtaaat tggttgaaaa agttcctagt tttgaaaata aataccaatt aattgttgca
     1261 ttacttgcaa cccaaaatgc ttttgaagcc ttagaacata ttcaattgat tcaagtaggc
     1321 atgcataaaa attgccctaa tttacttcag tgtaaagcta aggcgcaatt attattaaat
     1381 caaaagcaag ctgcttttga gaccgtaaaa gcgttaatga aaattgatcc tcttcaaaaa
     1441 aataaagcta tctgcattct atttgcacaa atcttggaag ccaatgggca ctatgaagcg
     1501 gcattaggtg aatatgaaaa ggcactagaa aatcgctatt gctttgagtc gcattactat
     1561 tatattcgtc tactcaagag attaaattac aaacatcgtg ccaaagatga agcaactaag
     1621 ctggtaatcc gctataaaaa attgaagcat acgcaaaaaa ggcaattacg taaatgggtt
     1681 attttgacgc atcaattaaa ttaaaaatat tcaaatcttg tcggtagctg tggccagagc
     1741 gcattgtcac ttaacacaca ggtcacattt tctaaagttt taatccaaac aaggaattct
     1801 tccttatccg aaaaatctct taaactgtgg ctatttgtcc accgatcgtg tgaaatatga
     1861 tcccaacgaa cccagatttt gtttgaagaa aaggaaatgc aaccacgctg tttctcgcgc
     1921 aatcctcttg tgcttggata atattgaaat atgggataat taccttcttg ctcaataaat
     1981 ttaataatta ttttataagc tgttgggatc gtagttttaa aaatattact tcttaataca
     2041 tgctgcactt cgatccaatc ggcatccgtt atccatttgc caggttgatt gacaggaaat
     2101 tttttttgtc gatttttttc taattctgag ctaattaaag attgttccga tgaatttaat
     2161 acgtgcattt ctaaaggagc aacatgcact tgcaccgaac ttgggatcat aattgaccca
     2221 tcgccgttga tcgaaatagg agaaatctca ggtttttgtt gtgttctata aaagctccaa
     2281 agataattca acatgaatct tccttaatgc tgcgtatgga taatttttgg atggaccaac
     2341 ttttcataat ctttatattg cattttataa agttctgaat gatttcctgc gttaaaagcg
     2401 atctccgaac catggactaa atcttcagaa acatatacat ccatatcata taaatttcca
     2461 aaaggtggca ttgccccgac ttcgcagtct ggaaatgccc cggagaattc ctcttctgta
     2521 gccaacacta ccggagttcc tatcaatttt tttaattctt ctaaatttac ttgatcgtgc
     2581 gcgcttatta caaccataaa taagtcgttg gttgttttac atatgaccgt tttcgccatt
     2641 tttttacttg ggatatggct tacctcacat aattgaagcg cagaaaatgc aatgggatgt
     2701 gaaatgactt cataataaac attgtgatta tctaagtaat tttttaatct ctgcgatgcc
     2761 ataattgccc cctgaaaaac cagtaggtca ttcactatag tagactactt aaatcttaaa
     2821 ctattaagcg aaccaatatg cgactgacaa atcagggtaa caaaaaatta tgttttttga
     2881 tttaaaattt cccaagatcc atcgggtcgg cggcaagcag ttccatacgc ttcttctact
     2941 ttattaccga tagtcacctc agactgatac tctcgacagt attctccacc tgaagctact
     3001 ccttcgcgca aatggactat tttgccatgg tgcccagaat ttggattttt ccatacgatg
     3061 gttctgccaa ccggtgcatt tgctgcttga tgaaatgcat catctgcttt ctctcgatcg
     3121 atttcatcaa gatattgtcc aacctcgctt cctataacag cgcctgcaaa tgctcccaac
     3181 atggttgctg caattttgcc actaccctta cctatatggc tagcagctag agcacctgtt
     3241 gctccaccta ttagcgcacc tgttgtcgta ttattgcctc gcgtttgttg gcatccgctc
     3301 acgcaaacca acgataatgt cagtgcacaa actaatattt ttttaaaccg cattttattt
     3361 ttatttccca tctactatgt attaaagatt gatgacttat ttaatgctga tttttttatc
     3421 ttgtcaattc tctatcataa ttttagatgg ttaactcttg tagttaattc tactatttta
     3481 aattgattaa agtattcgtt tctcgtgtat tctaaaagat aacaagccaa ttcgggggct
     3541 tgctcttgag cataatccca caaatttctt tccgaagata aatcaaatag atcatcaaaa
     3601 taaagctttc gttcttgctt ttcaaagcgc tcttttaaaa atttaaatat atccagtctt
     3661 ttatgatcta ttaaaaaatg aaaaggcgat ttttcactcg cgctttctgg cgtagacgtt
     3721 atttgtttta tatcaattaa attcgcacgg tattttatta tttttttata aaaatcctct
     3781 gcactccccc attgggctat gtaagataaa ctcttttgta ttggaaaggc tttgagaaaa
     3841 ggttgcgaaa ccgcaatgtt gcaatcaata ttttgtccat tagaggtcaa ttcaagcaaa
     3901 ttaatactat tttgaaatcc agcttctcta agaagcattt gggcaatagt ataaaatatt
     3961 ttaatattta agcgaaaatt ttcatcttgc tcagttaaaa aaacaggatc cattaacgct
     4021 aacaaatcct gcattcgtaa tttttttaaa cgccattcaa tatcatcgct ttcaagaata
     4081 tttcttcgta gctttgctaa accttcaaag taatggcttt tcttgataac gacgtgcttt
     4141 tttatctctg taaaggtaag ttgtgaagaa ttagaaaaat tatccatgcc tacccgccat
     4201 gcataaacac ctttttgagc aagtttttct aaaaaagata ccccagcatt atcactttgc
     4261 gtatttacat ataaatattg gagactagaa taatgctttt cttctaaaag actattcaaa
     4321 attgatccat tgggtgaatc aacaatataa tgccataatg gtatttgatg aaaaccataa
     4381 cgaatagtgt gcaaatcgat tttatagtta cgtatatatt ctacatacaa taaaaaagca
     4441 gataaatttc caatatctat ggcgtgatga atagcggtct tgccattttt ttgtttatag
     4501 cagggatcac atcctgcatc tatacatatt tggatagctt cacaattgct aacattcgct
     4561 tcaaataata aaggatattt tggcagtgca ttgacgatat taaaatagct attagcacaa
     4621 ggtgagcttg cgagttgctc acaacattgt ttaatttgcc caaaaagaaa tggtaataac
     4681 tttatttttt ttgtgatcac taaattcagc aaactattct catcattctg tatgaaattt
     4741 aacacatgtt gtggatgacc ttgtacgaga gtttgaaata actcacatga actttcgtcc
     4801 tcaagtaatt gctttaattt gctgagcaac aagtttggat aaataaagaa gttaatactt
     4861 gagcgaaaat tttttaatgc ttgattttta aattcagctt gatctgcaga tttataccat
     4921 gcggaggtaa taatttcgta accttcattt tcgtcaaatt tatttaatat tttttgaaag
     4981 aactgacgta ccacaaaact tgtatattga acgcttatgc agtgtttcag ttcgtgttca
     5041 ggaaggtgtt tgaatttagt caactcttcc atccattctg aaaaataatg cgtaagctta
     5101 tcaagataaa atgcctcttt tttatccaaa gcagttgtta gtttgagatt ttttgacacc
     5161 gaatgaaatg ttccactata aaaatcttta gggttgcgaa atttttcatg ccaaagacgt
     5221 gctgcgttaa aaccactatg aatcaacaaa ttgaattttt tatcgtctaa atgaaattcc
     5281 gtagttttaa tattttcaat atagatttgt acggtattgt ctggatgtgt tttgtttttt
     5341 ttataactat cttctggctg caatttacca tatttatagc gcagcaatat acgaatataa
     5401 tcagaaaatg tccagtatgg ttggggtctt acactttctt tcttatcaaa agaattttgc
     5461 gcaatttttt gaacacgttc agaataagga gtaatatttt catctaattg cgtaatttta
     5521 gacacaatgc taaatcctaa aatactagga tttatcatta cagattctgc ataatcctta
     5581 tattgaatat tctgcaaagg ataagaaatt tcacagaatt tatcactatc aaataccgca
     5641 ttcggaaaat tgtttagaat tcc
//
LOCUS       DASTIB010000189         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1194465, whole genome shotgun sequence.
ACCESSION   DASTIB010000189 DASTIB010000000
VERSION     DASTIB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194465"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..587)
                     /locus_tag="VFP93_03250"
     CDS             complement(294..587)
                     /locus_tag="VFP93_03250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9843656.1"
                     /translation="MHVNAHPHIVHDETNHQFIATVNGDQAYLAYTVSSNEVLDFRST
                     FVPVKLRGQHIGQDIVEYALEYAKTNQFKIIPTCSFVRWIIRNNPQYEDLVKS"
     gene            736..1446
                     /locus_tag="VFP93_03255"
     CDS             736..1446
                     /locus_tag="VFP93_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase-C39 like family protein"
                     /protein_id="HET9843657.1"
                     /translation="MFKVEISTQPDDETCGPTSLHAVYQYYGDAIPLEAVIHEVSRVR
                     NGGTIAPLLGLHAIQRGYEAYIYVYNLDVFDPTWFYPKKLNSTELLKKLALQETYKNS
                     PRFVEFSSAYRQFIEVGGEIRFQDLTVALLKEYFNANIPILTGLSATYLYQSSREVET
                     KDGLGIFDDLRGLPLGHFVILCGHDENKRKIVVADPHRENPISHDNYYKVPVSRLINS
                     IMLGVLTYDANLLIIKPK"
     gene            1453..>2434
                     /locus_tag="VFP93_03260"
     CDS             1453..>2434
                     /locus_tag="VFP93_03260"
                     /inference="COORDINATES: protein motif:HMM:NF025759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HET9843658.1"
                     /translation="MQTILVTDQAKLWDFANEFTTVVSAIQYISDNNFTNTDSIRVIN
                     LCKSYQYQSLGYYVSLLAEARGHKINPSTVTIQDINKFLLPLFEDHSLDEEIQRSLKN
                     IKSNQFILSIYFGKNIAKQYEKICHKFYNLCPLPLFRVYFRKKKTWKIIKIAHISILD
                     VPDSHREFLEASANAYFNKKRFRQYKDKKFNFDMAILYNEAEKTAPSNPAAIQKFIQA
                     GEELGINIEIIDKGDLRFIAEYDALFIRETTAVNHHTYQFSRRAEAEGLVVIDDPGSI
                     LKCTNKVFLSELLAKHKVPHPKTHLISKHKWKEYIHNIPTPCVLKKPDGAF"
BASE COUNT          856 a          403 c          397 g          778 t
ORIGIN      
        1 gacatttcat tttcgtaaat gcataattta atttgaatca aaaaaataaa aagaggtgcg
       61 agacaattaa aaaataaagg aggaaaatga ttagataaat taaaattttg attcttatag
      121 aattataaaa aaaaatttgc cagaactact attcatctaa atcaatttta atgacagaat
      181 cagaaaaaac gtttgacgtt cgaaaaaata ttttaagaaa gttacgcagc ttttagcgaa
      241 gtgtacaaaa tatattgcat cgcagtatgc acatttcaaa atgacggata aaattaactt
      301 tttaccaaat cctcatactg aggattattg cggatgatcc atcttacaaa agaacaagtg
      361 ggaataattt taaattgatt cgtttttgca tattctaagg catactcaac aatatcttga
      421 ccaatatgtt gtccacgaag tttcacagga acgaaagtgc tcctaaagtc gagaacttca
      481 ttggagctga ctgtataggc caaataagcc tgatctccat ttacggttgc gataaattga
      541 tgatttgttt catcgtgtac gatatgggga tgcgcattaa catgcatttt ttattactta
      601 gctgtgcttt tataaaattt tagtaaaaaa tttttaatta gacgaattat ttaccgtttt
      661 attgaactta tccatgtaat tacccatact aatatataga acatcaatag gttttaggat
      721 tttatcagtt agtgtatgtt caaagtagaa atcagtacac aaccagatga cgaaacctgc
      781 ggccccacta gccttcatgc tgtttaccag tattatgggg atgcaattcc gttggaagct
      841 gtgattcatg aagttagtcg agtacgtaat ggtggcacca tagcgccact tttaggttta
      901 catgccatcc aaagaggata tgaagcttat atatatgttt ataacctaga tgtttttgat
      961 ccaacctggt tttatccaaa aaaacttaac tctacggaat tattaaagaa gttagctcta
     1021 caagaaactt ataaaaattc accccgattt gttgagtttt cttccgctta tcgtcaattt
     1081 atagaagtgg gtggtgaaat tagattccag gatttaacgg tggccttatt gaaagaatat
     1141 tttaatgcta atatacccat attaacgggc ttaagcgcta cgtacttata ccagagttct
     1201 cgcgaggtag aaaccaaaga cggtttagga atttttgatg atttgcgcgg actaccctta
     1261 gggcattttg tcattttatg cggtcatgat gaaaataaaa gaaaaattgt ggttgccgat
     1321 ccgcatcgcg aaaatcctat ttctcatgat aactattaca aagtacccgt aagtcggcta
     1381 ataaattcta taatgctcgg tgttttaacc tatgatgcaa accttcttat tattaaacca
     1441 aaataactat ccatgcaaac aatactcgta accgatcaag ctaaactgtg ggatttcgct
     1501 aacgaattta caaccgttgt atccgctatt caatatatct cggataacaa ttttaccaat
     1561 acagattcga ttcgcgtaat taatttatgt aaaagttatc aatatcagtc attagggtat
     1621 tatgtgtcac ttctggcaga agcacgtggg cacaaaataa atccttctac agttactatt
     1681 caagatatca ataaattttt attacccctt ttcgaagatc actcactcga tgaagaaatt
     1741 cagcgctcat taaaaaatat taaatccaat caatttattc taagtattta ttttggaaaa
     1801 aatattgcga aacaatatga gaaaatatgc cataaatttt ataatttatg tcctttacct
     1861 ctttttcgtg tgtattttag aaaaaagaaa acgtggaaaa ttataaaaat agcacacatt
     1921 tctattctgg atgtaccgga tagtcatcga gaatttctag aagcttcggc gaatgcctat
     1981 tttaataaaa aaaggtttag acaatataaa gataaaaaat ttaattttga tatggctatt
     2041 ttatataatg aagcagagaa aaccgctcct tccaacccag cagctatcca aaaatttatt
     2101 caagctggag aagaattagg aatcaatata gaaataatcg ataaaggtga tttacgtttt
     2161 attgcagagt acgatgcttt atttattcgc gaaactactg ccgtaaacca tcacacctac
     2221 caattttctc gaagagctga agccgaaggt ttagtggtaa ttgatgaccc gggctcaatt
     2281 ttaaaatgca ctaataaggt ttttttatcg gaattacttg caaaacacaa agtgcctcat
     2341 cccaaaaccc atttgatttc aaaacataaa tggaaagaat atattcacaa tattccaaca
     2401 ccttgcgttt taaaaaaacc ggatggcgca tttt
//
LOCUS       DASTIB010000190         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1230303, whole genome shotgun sequence.
ACCESSION   DASTIB010000190 DASTIB010000000
VERSION     DASTIB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230303"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFP93_03265"
     CDS             complement(<1..938)
                     /locus_tag="VFP93_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9843659.1"
                     /translation="MNIIRKLFGPAVSKIIMPAQKKEDLYGPVRAIQRDGNVNILNHF
                     ISAPKASINIAEFTSAPEVPITHKKIVIFSVGNNGNYEQYFYDYVNLARENPHWHIVG
                     FNYRNVLASKGKAESEQDWINDAKSVILHFRNQGCENENILLCGHSLGGAIVTMAASQ
                     LYQEDQHAAKTNGKNPAGVKCVRCINDRSFSNLSEEIVISLLGHTRSSIINAVIFGVL
                     VASISLLWVFCFPAIGGLTILSSLNLAGITAGAVLGVSLLKDTIAYALVRPWVTMLAD
                     LTFGRMDALSQYQKIPEESKSYLIAKNDSVVKKAAS"
     gene            complement(1069..1845)
                     /locus_tag="VFP93_03270"
     CDS             complement(1069..1845)
                     /locus_tag="VFP93_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HET9843660.1"
                     /translation="MKLLKLFACAIALFTSFLVVAEVPKEIDEKIRKNFKKSLPSLKI
                     DTIKSSPIPKLYEITSGPIIMYVTEDAQFAISGDILDLKDGETNVTEEARKAARIHAL
                     NQLGEKHMIVYPAGKPKYEVTVFTDLDCVYCRKFHAQMDQLNNMGITVRYVAFPRAGA
                     QSTSFEKAVNVWCAENPQQALTKAKQGQPVPKATCDTHKINEQFHLGALSGISGTPTL
                     ILQDGTLVPGFYAADTLLGLLQETTNTKKAHVNKNKKKNS"
     gene            complement(1854..2381)
                     /locus_tag="VFP93_03275"
     CDS             complement(1854..2381)
                     /locus_tag="VFP93_03275"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9843661.1"
                     /translation="MQVLLNSPRLKLQREAYSAIIASPVGPLGLKVEQGKLKALDFLS
                     PNTPLILNADSTVDSYIRALHNYFSNSKLISIPLTSLSLSGTPFQLKVWDALTKIPAG
                     STITYGHLAKALNSSPRAIGGACKSNPCPIIIPCHRVVAAKHLGGFAGCLTGSKIKLK
                     HWLLQHEASFIKNCI"
     gene            complement(2365..2745)
                     /gene="rplS"
                     /locus_tag="VFP93_03280"
     CDS             complement(2365..2745)
                     /gene="rplS"
                     /locus_tag="VFP93_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9843662.1"
                     /translation="MQEIENEQLKTNIPAFNSGDTVIVWVKVKEAGRERLQAFEGVVI
                     AIKSRGVNSSFTVRKISHGEGVERVFQTHSPLIDRIEVKRHGKVRQSRLYYLRNLAGR
                     AARIKEDLDRRTKSDNTGDNAGTA"
BASE COUNT          799 a          572 c          492 g          890 t
ORIGIN      
        1 aagctagctg ctttttttac taccgaatca ttttttgcta ttagataact tttgctttct
       61 tctggtatct tttgatattg gcttaaggca tccatgcgac caaacgttaa atctgctaac
      121 attgtaaccc aaggtcttac cagcgcatac gctattgtgt cttttaataa actaacccct
      181 aaaactgcac cagctgtaat tcctgctaaa ttcaagctgc ttaaaatagt taaacccccg
      241 attgcgggaa aacaaaaaac ccaaagcaga gaaatacttg cgacaagtac gccaaaaatt
      301 accgcattaa taatagaaga tcgcgtatgt cctaataaac ttataacaat ttcttcagat
      361 aaattagaaa aagagcgatc gttaatacaa cgaacgcatt taactcctgc tgggttcttg
      421 ccatttgttt tggcagcatg ttgatcttct tggtaaagtt gacttgccgc catcgtgaca
      481 atggcgcccc ctaaggagtg tccacacaat aagatatttt cattttcgca accttgattt
      541 ctaaaatgca gtattaccga ttttgcatca ttgatccaat cttgctcact ttccgcttta
      601 cctttacttg ccaagacatt tctataatta aatcctacaa tgtgccaatg cggattttcg
      661 cgcgccaaat taacataatc gtaaaaatat tgctcataat taccattgtt acccacagaa
      721 aatattacaa tttttttatg ggtaatgggg acctcaggtg cggaagtaaa ctcggcgata
      781 ttaatcgagg ccttgggcgc ggaaataaag tggtttaaaa tatttacatt gccatccctc
      841 tgtattgcac gaacaggacc ataaagatcc tctttttttt gcgctggcat aatgatcttt
      901 gaaacagcag gtccaaaaag ctttcgaata atattcatat tttcttatcc ttaagaaacc
      961 aggtaaattg ttattcaact taaaaaaaaa gaactacaca tgttgagtat acggtgtttt
     1021 taaaaagcgg aaaaggtaga tatggatccc gcaaggggat ccctattttt agctgttttt
     1081 ctttttattt ttattgacgt gtgctttttt ggtatttgta gtttcttgta atagtccaag
     1141 caacgtatca gcagcataaa agccaggcac taaggttcca tcttgtaaaa ttaatgttgg
     1201 agtacctgaa atgcctgata acgctcccaa atgaaattgc tcattaattt tatgcgtatc
     1261 acacgttgct tttggcacgg gttgaccttg ctttgctttt gttaaagctt gctgtggatt
     1321 ttccgcacac caaacgttta cggctttttc aaaagatgta ctttgcgctc cagcccgcgg
     1381 gaaagcaaca taacgcaccg taatacccat attgtttaat tgatccattt gcgcatgaaa
     1441 ttttcggcag tacacacaat ctaaatccgt aaagactgtt acttcatatt tgggcttccc
     1501 ggcaggataa acaatcatgt gtttttcacc caactgattt aaagcatgaa tacgagcagc
     1561 tttgcgagct tcttctgtta cattcgtttc tccatcctta agatccaaaa tatcacccga
     1621 tattgcaaat tgcgcatctt ccgtcacata cataatgatg ggaccggaag taatttcgta
     1681 cagttttgga attgggcttg atttaatagt gtcaattttc aaacttggca aacttttttt
     1741 aaagtttttg cgtatttttt catctatttc tttgggaact tccgccacta ccaagaaact
     1801 cgtgaacaag gctatagcgc atgcgaacaa ctttaataat ttcataatca tccttaaatg
     1861 caatttttta taaaactggc ttcatgttgt agtagccaat gtttcagttt tattttagaa
     1921 cctgttaagc atcctgcaaa acctcccaaa tgtttagccg ctacaacacg atgacaggga
     1981 ataattatgg ggcaaggatt gcttttacag gctccgccaa tcgctcttgg tgagctattt
     2041 aacgcttttg ctagatgccc gtaggtaatg gtgctgcctg caggtatttt tgtcaatgca
     2101 tcccaaacct tcaattgaaa tggtgtacct gacaaagaaa ggctggtaag gggtatggaa
     2161 attaatttgg aattagaaaa ataattgtga agtgctctga tgtaactatc aaccgtagaa
     2221 tctgcattca agatcaatgg ggtgttaggt gataaaaaat cgagcgcttt taattttcct
     2281 tgctcaactt ttaaacccaa tggccccaca ggagaagcaa tgattgcact atacgcttct
     2341 cgctgtagtt ttagacgagg cgaattaagc agtacctgca ttgtcgcctg tgttatcgct
     2401 tttcgtgcga cgatccagat cttctttaat acgcgctgca cgccctgcta aattacgtaa
     2461 gtagtacaat cttgattgac gtactttacc gtgacgtttc acttcaatgc gatcgattaa
     2521 agggctatgt gtttgaaaaa ctctttcaac cccttctcca tgagaaattt tccgcacggt
     2581 aaaagaagag ttaactcctc tgctcttaat agctataaca acaccttcaa aagcctgcaa
     2641 ccgttctctg cctgcttcct tcactttaac ccaaacaatg actgtatctc cggaattgaa
     2701 cgccggtata tttgttttta attgttcgtt ttcaatctct tggattaaag tac
//
LOCUS       DASTIB010000191         3686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1374934, whole genome shotgun sequence.
ACCESSION   DASTIB010000191 DASTIB010000000
VERSION     DASTIB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374934"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..630
                     /gene="orn"
                     /locus_tag="VFP93_03285"
     CDS             70..630
                     /gene="orn"
                     /locus_tag="VFP93_03285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET9843663.1"
                     /translation="MTVNADHLIWIDLEMTGLDPDKDKIIEIATIVTDSQLNIVAEGP
                     NLVIYQSSEIMNAMDEWCTKQHALSGLTQKVLESHVNEREAEKQTLAFLKQFVKPRYS
                     PMCGNTICQDRRFLYRNMPELEAFFHYRHLDVSTVKELAKRWMPKIYNSMEKEAKHLA
                     LEDIKESIEELKYYRQHFFIIPEIQK"
     gene            627..2129
                     /locus_tag="VFP93_03290"
     CDS             627..2129
                     /locus_tag="VFP93_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9843664.1"
                     /translation="MTLKLYDVQTITKIETKAIQEWGVPAFELMQRAGRVAFEVIREQ
                     WSLAKKFAVICGSGNNAGDGYVLAKLLLEDDFKVDVYSVLDPSSALSGVAELARQEFL
                     STGNSIKVLPNRLEAVDVIVDALLGIGFIPPLRTEWQKAIQLMNRSEKPIFSLDIPSG
                     LDPNTGAVSKETVKASVTLSYIGHKHILLSGPAVNYAGQVVVNDLGLADILESIPWDG
                     EIIDFPKTLSFLEHRKKSSHKGDYGHVLVIGAGKAGFSGAVGLAGSSALHAGAGAVSV
                     FVHPEALSLLSRVPLELMCYSKEEEVPWQKANVCVLGPGLTESPWAKTHFDKALRMHK
                     PMVVDADALNLLAQYPHKREEWVLTPHPGEAARLLGITVEQVEQNRRDAVKSLQARFG
                     GVVVLKGAGTLVQGAREDFHLCGAGNPGMAASGMGDILAGLIGGLMAQGMPLYESALL
                     GVQVHASAGDIEQSLGERGMMASDLLLHIRSLLNPTTQKIESNVSKFFNI"
     gene            2104..2589
                     /gene="tsaE"
                     /locus_tag="VFP93_03295"
     CDS             2104..2589
                     /gene="tsaE"
                     /locus_tag="VFP93_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005504261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET9843665.1"
                     /translation="MFQNFLISSEAHMQELGSALAFACQNQKINSCVIYLHGDLGAGK
                     TTCIRGFLRAMGYKGSVKSPTYTLVEPYDFAECKVYHFDFYRLKYPQECEEMGIRDYV
                     HPGGYCLIEWPEKAVGYIPIALLSIKIAINSAEERNVNVESHTSQGEIILTTLRDRLT
                     K"
     gene            2586..>3686
                     /locus_tag="VFP93_03300"
     CDS             2586..>3686
                     /locus_tag="VFP93_03300"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9843666.1"
                     /translation="MKKAALFLLGCCLSFYSFAAVQLDGLRIWPSPDKTRVVFDLSAA
                     SDYKLLTLQNPDRIVIDLRNTHANASLEGLVYTKTGIEKIRYGNKDNHLRIVLQLSRK
                     FKPNAFLLKPYGNYGHRLVLDLEIPEHSKKEPDNVEITPSKPIASMNTLLSRDFVIAI
                     DAGHGGEDPGAIGRTYKTREKDVVLSVAKKLQQLINQQPGMRALLIRDGDYYVGLRKR
                     TQSARKQGADLFISLHADSYQNTTAQGASVFVLSDHGASSEAARWLAERENRTDLIGG
                     VSLDDKGDLLASVLLDLSQTANQSASEELGQLILKALGKVTQLHHRTVQRAGFVVLKS
                     PDVPSILVELGFLSNNKGEDKLRKESHQKELAV"
BASE COUNT         1151 a          660 c          838 g         1037 t
ORIGIN      
        1 tttacaaact aaaatatgtt agataaataa acacttactg tcaatccatt taatttaagg
       61 atccgaagaa tgaccgttaa tgccgaccat ctcatctgga ttgatctgga aatgacggga
      121 cttgatccag ataaagacaa aatcattgaa attgccacca ttgttacgga tagccaactt
      181 aatattgttg cagaaggacc caacctagtc atttaccaaa gcagtgaaat tatgaatgct
      241 atggatgaat ggtgcactaa gcaacacgct ttatcaggat taacccaaaa agttctagag
      301 agtcatgtta atgaaaggga agcagaaaaa caaacccttg ctttcttaaa gcaatttgta
      361 aagccaagat attcacccat gtgtggcaac accatatgcc aagatcggcg ctttttatat
      421 aggaatatgc cggagctaga agccttcttt cactataggc atttagatgt aagcaccgta
      481 aaagaattag caaaacgttg gatgcctaaa atatacaaca gcatggaaaa agaagcgaag
      541 catttagcgc tagaggacat taaagaatcg atcgaagaat tgaaatatta tcgacaacat
      601 ttttttatca ttcctgagat acaaaaatga ctttaaaatt atatgatgtg caaacaatta
      661 caaaaataga aacaaaagct atacaagaat ggggagttcc ggccttcgag cttatgcaac
      721 gcgcaggtcg tgttgcattt gaagtcattc gtgagcaatg gtctttagct aagaaatttg
      781 cagtaatatg cggatcgggt aataacgcag gggatggata tgttctagca aaacttctcc
      841 tagaagatga ctttaaagta gatgtttatt ctgttcttga tccaagctcc gcactttccg
      901 gtgtagctga gcttgcacgt caagaatttt tgtcaaccgg aaactctatc aaagtattac
      961 ccaatcgctt agaagcggta gatgtgatag tggatgcatt gctcggtatt gggtttattc
     1021 caccgttaag aacagaatgg caaaaagcca ttcaattaat gaatcgtagc gaaaaaccca
     1081 ttttctcgct cgatatacct tcaggtcttg atcccaatac aggcgctgta tcaaaagaaa
     1141 cggtaaaggc cagtgttact ttgtcgtata taggccacaa gcatatttta ctatcggggc
     1201 cagccgtcaa ttatgcgggt caagtagttg taaatgatct tggtcttgca gatattttag
     1261 aaagtatccc atgggatggg gaaattattg attttcctaa aacacttagc tttttagagc
     1321 atcgaaaaaa atcttcccat aagggagact atggtcatgt tttggtgatt ggtgcaggaa
     1381 aagcaggctt cagtggcgct gtaggtttag ccggctccag tgccttacat gcgggtgccg
     1441 gtgcggtatc ggtattcgtt cacccagaag cactgtcgct attaagtaga gttcctttag
     1501 agcttatgtg ttatagcaaa gaagaggaag tgccctggca aaaagcaaac gtgtgcgttt
     1561 taggtccggg gttaaccgaa tccccttggg cgaaaacaca ttttgataaa gcattacgca
     1621 tgcataaacc tatggttgta gatgcagatg ctttaaattt attggcgcaa tatccgcata
     1681 agagagagga atgggtttta accccccatc caggtgaggc agcacgactt ttaggtataa
     1741 cggtagaaca agttgaacag aatcggcgtg atgcggtgaa aagtctgcaa gcgcgctttg
     1801 gcggcgttgt ggtgttaaaa ggtgcaggaa ccttagtgca aggagcgcgg gaggactttc
     1861 acttatgtgg tgccgggaat cctgggatgg ctgcgagcgg aatgggagat attttagcag
     1921 gtttaattgg gggattgatg gcgcaaggaa tgcctttata tgaatctgcg ctattgggcg
     1981 ttcaagtgca tgcaagtgca ggcgatatag aacaatcctt aggcgagcgt ggtatgatgg
     2041 caagtgactt attgttgcat attagaagtt tattaaatcc aacgacacaa aaaatagaat
     2101 ccaatgtttc aaaatttttt aatatctagc gaagcacaca tgcaagaatt gggctctgct
     2161 ttagcattcg cttgtcagaa ccaaaaaata aattcctgtg tcatatattt gcatggagat
     2221 ttaggggcag gtaaaactac ctgcattcgt ggcttcttac gtgcaatggg atataaaggt
     2281 tctgttaaaa gtccaacgta tacgcttgtt gaaccttatg acttcgcgga atgcaaagtt
     2341 tatcactttg atttttatcg acttaaatat ccacaagaat gtgaagaaat gggtatccgt
     2401 gattacgtgc atcctggtgg gtattgttta atcgaatggc cagagaaagc cgtgggttat
     2461 atcccaattg ctttgttaag tattaaaata gctataaatt ctgctgagga aagaaatgta
     2521 aacgtggaat cgcatacaag tcagggagaa ataattctta ctactttacg agatcgtcta
     2581 acaaaatgaa aaaagcggcg ttattcttat tggggtgttg tttaagtttt tatagttttg
     2641 cagcggtgca attagatggg ttgcgtatct ggccatctcc tgacaaaaca cgtgtggtat
     2701 ttgatttgtc ggccgctagt gactataaac ttttaacgtt gcaaaatccg gatcgtattg
     2761 taattgatct tagaaacaca cacgctaacg caagcttaga aggtttggtt tataccaaaa
     2821 ccgggatcga aaaaattcgt tatggtaata aagataatca cttacgcatc gtattacaat
     2881 tatcccggaa atttaaacca aatgcgtttt tgttaaaacc gtatggcaat tatggccata
     2941 gattagtcct cgatctcgaa attcctgagc acagcaaaaa agaacctgac aatgtggaaa
     3001 ttaccccttc caaacccatt gcttcgatga atacattgtt gtcacgtgat tttgtcattg
     3061 ctattgatgc aggtcacgga ggcgaagatc cgggtgcaat cggtcgaacc tataaaacgc
     3121 gtgaaaaaga tgttgtgtta agtgttgcga aaaaattaca acaattgatc aatcaacaac
     3181 cgggaatgcg agcattgtta attcgagatg gtgattatta tgttggatta cgtaagcgta
     3241 ctcaatctgc gcgaaaacaa ggcgctgatc ttttcatttc tttacatgca gatagttatc
     3301 aaaacacgac cgctcaaggc gcttcggttt ttgtgctatc cgatcatggg gcaagtagcg
     3361 aagcagcgcg ctggttggca gaacgtgaaa accgtaccga tttgatagga ggcgttagtc
     3421 tagacgataa aggtgatttg ctggcatctg ttctcctaga tttatctcaa actgcaaacc
     3481 aatcggcaag tgaagaattg ggtcagctta ttttaaaagc tcttggaaaa gttacgcaat
     3541 tgcatcatcg tacagtacaa agggctggat ttgtggtttt aaaatctcca gatgtaccat
     3601 ccatattagt agaacttggc tttttgtcaa acaacaaagg cgaagataag ttacgaaaag
     3661 aatcgcacca aaaggaacta gctgta
//
LOCUS       DASTIB010000192         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1392063, whole genome shotgun sequence.
ACCESSION   DASTIB010000192 DASTIB010000000
VERSION     DASTIB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392063"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFP93_03305"
     CDS             complement(<1..512)
                     /locus_tag="VFP93_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HET9843667.1"
                     /translation="MEFTSSLTEAILLKRYKRFLADVAVSKTDRRTIYCPNTGAMRGC
                     NILGSRVWFSSSTNLRRKYPDTWELVEIDGGYLVAINTQRTNQLILEAIASGTITEIQ
                     GYKNIEREYALTHYNNRFDFRFSNHEYDKTPCFMEVKAVTLGDEICRGFYPDSNAEIT
                     THQLKALIQL"
     gene            847..1353
                     /gene="dksA"
                     /locus_tag="VFP93_03310"
     CDS             847..1353
                     /gene="dksA"
                     /locus_tag="VFP93_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HET9843668.1"
                     /translation="MATRTPQNKSGIEAPSPKKKIEDKNPAPTTTRGVEPYQPSQDEP
                     YMGDAQRKHFRNLLQIWKAALLKSGDKTLSGLRQLGELGEVPADPIDVATNEGAIALD
                     LRARDRERKLINKIDEAIQRIDNKDYGYCDECGVDIGIRRLEARPTATLCIDCKMLDE
                     VREKQRRS"
     gene            1369..2031
                     /locus_tag="VFP93_03315"
     CDS             1369..2031
                     /locus_tag="VFP93_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HET9843669.1"
                     /translation="MDVLTAIQERRSVRAFLAKPVSREVIIKIFETARFAPSGVNHQP
                     WNVLILNEAMREKLAQVIVEKREANIPENPDYNYYPTLWFEPYKTRRKKCGKDLYQSL
                     HIAYEDKEARKQQWYKNYYFFGAPIAMIFYIHKDLSTGSWMDFGGFLQTVMIAARSYE
                     IETCPQAALAEYPDVVRELCGIDQNYKIVCGMALGYADWNNPVNQYRTEREAVNTFIK
                     WT"
     gene            2180..2425
                     /locus_tag="VFP93_03320"
     CDS             2180..2425
                     /locus_tag="VFP93_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843670.1"
                     /translation="MTNPIFSGLQAFWGIWIIGQFWCFLYAGLGLAIPMGLVGLAFGW
                     LQIILITQGQIQPHLGTAGFGQALKTILGKIYSIVTF"
     gene            complement(2450..>2968)
                     /gene="phaR"
                     /locus_tag="VFP93_03325"
     CDS             complement(2450..>2968)
                     /gene="phaR"
                     /locus_tag="VFP93_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HET9843671.1"
                     /translation="IRIIKKYPNRRLYDTAISSYITQGDVKLLVLDNIPIQVIDARTK
                     NDITQNTLLQIILEQEEKGPPIFTTEILQQLIRFYGGSLQNVISRTFEQIMHFFSEKQ
                     NMLKQGMTDELNDIVQKDPFSLIADRSKKNEESWTLMQQQWVQALTNPHSVTESNQRN
                     ETIIESQAQNSD"
BASE COUNT          911 a          538 c          577 g          942 t
ORIGIN      
        1 tttaactgaa taagggcttt taattgatgg gtagttattt ccgcattaga atctggataa
       61 aaaccacgac aaatttcatc accaagtgtc acggctttaa cttccataaa acaaggcgtc
      121 ttgtcgtatt cgtgatttga aaaacgaaaa tcgaaacgat tattataatg ggttaacgca
      181 tattctcttt ctatattttt ataaccttgg atttccgtaa tagtgccact ggcaatagcc
      241 tctaaaataa gttgattcgt tctttgggta ttaatcgcta ctaaatatcc tccatcgatt
      301 tccacaagtt cccatgtatc gggatatttt cgtcgtagat tcgtggaaga ggaaaaccac
      361 acgcgtgaac ctagaatatt acaacctcgc atggcacccg tgttaggaca ataaatagtg
      421 cgacgatcgg tttttgaaac tgcaacatcc gctaaaaatc gcttatagcg tttaagaagt
      481 atagcttcag taagcgagct ggtaaattcc atgcaacaac caaacgacca acttaagctt
      541 aatgtcggcg aattccggta agtgctttag ataaatctga aaatccattt agctgccaca
      601 atttacaggt attttcattc agcagtttca gagaatcgct atagcttgct cttttttata
      661 agtgtatatt cgcttctgct gtttttattg aagttgaaaa tatgggaggc actaatttag
      721 tgtgctatcc tataattttc aggaattttt tgtctatacg tttaagtttg gaaagtctta
      781 cgtatatact taaatcttaa tgccaggagg gcattatgtg taaaaacccc aagggaggag
      841 acacctatgg caacacgtac gccacaaaat aagtcaggca tcgaagctcc ctcacccaag
      901 aaaaaaattg aggataaaaa tcctgcgccc acaactactc ggggggttga accttatcag
      961 cctagtcaag atgaacctta tatgggtgat gctcagcgca aacattttag aaaccttttg
     1021 caaatttgga aagcagcttt attaaaaagc ggagataaaa cactttcggg tttaagacaa
     1081 ctgggtgaat taggcgaagt accggcagat cccatagatg tcgcaacgaa tgaaggggca
     1141 attgccttag acttacgtgc acgtgaccgt gaacgcaaac tcatcaataa aattgatgaa
     1201 gctattcagc gcattgacaa taaagattat ggatattgcg atgaatgtgg tgtagatatt
     1261 ggcattcgac gattggaagc tagacctacc gctacgttat gcattgattg caaaatgctc
     1321 gatgaagttc gtgaaaaaca aaggcgctct tgatttaggc aaaatatttt ggatgtatta
     1381 acagctatac aagaaaggcg atcggtgcgc gcctttttag ctaaacctgt ctctcgtgaa
     1441 gtgataatca aaatttttga aacagcacgt tttgcacctt ccggcgtaaa tcatcaaccc
     1501 tggaatgttc tcatcttaaa cgaagctatg cgcgaaaaac ttgcacaagt tattgtcgaa
     1561 aaaagagaag ctaatatacc tgaaaacccc gactataatt actatcctac gctatggttc
     1621 gagccctata aaacaagacg taagaaatgc gggaaagatt tgtaccaatc attacatatc
     1681 gcttatgaag ataaggaagc ccgcaagcaa caatggtata aaaactacta tttttttggg
     1741 gcacccatcg caatgatatt ttacatccat aaagatttat ccacaggttc gtggatggat
     1801 tttggaggat ttttacaaac tgtgatgata gctgccagat cctatgagat tgaaacttgt
     1861 ccacaagcgg cattggcaga atatccagat gtcgtaagag aactttgtgg aatagatcaa
     1921 aactataaaa ttgtttgcgg catggcgtta ggttatgctg attggaataa tccggttaat
     1981 caatatagaa ccgaacgaga agctgtaaat acctttatca aatggaccta agtcactctt
     2041 caaacttgac taaaatgctg cataagcgat aaaatacatc taagtctcct ttcagtactc
     2101 ggtcatgaca aaaaatcttt attttttcat tttttactgt ggtctattga ttagtaccct
     2161 tattttaaca atctttgtaa tgactaaccc aattttttct ggtcttcaag ccttttgggg
     2221 aatttggata attggtcaat tttggtgttt tttatatgca ggcttgggtc ttgcgattcc
     2281 tatgggttta gtaggattag cttttggctg gttacaaatt attttaatca cacaaggcca
     2341 aatccaacca catcttggca cagcaggttt tggtcaagca ttaaaaacta tattaggaaa
     2401 gatctactct atagtaacat tttagttttc tcttaaagcc gttgattaat taatcactat
     2461 tttgtgcttg gctttcaata attgtttcat tacgctgatt tgattccgtc acagaatggg
     2521 gatttgttaa cgcttgtacc cattgctgtt gcataagggt ccagctttct tcgtttttct
     2581 ttgaacgatc tgctattaaa gaaaaaggat ctttttggac aatatcatta agctcatccg
     2641 tcatgccttg ttttaacatg ttttgctttt ctgaaaagaa atgcattatt tgttcaaacg
     2701 ttcgactaat gacgttttgt aaagatccgc cgtaaaaccg aatcaattgt tgtaatattt
     2761 ccgtcgtaaa aattggaggg cctttttctt cttgctcaag aatgatttgt aaaagtgtat
     2821 tttgggtaat atcgtttttt gtgcgggcat cgatgacttg aatgggaata ttgtctaaaa
     2881 ctaaaagttt cacatcccct tgagtaatat aactgctgat ggcggtatcg tataatctac
     2941 ggttgggata tttttttata atccgtat
//
LOCUS       DASTIB010000193         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_852312, whole genome shotgun sequence.
ACCESSION   DASTIB010000193 DASTIB010000000
VERSION     DASTIB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852312"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="VFP93_03330"
     CDS             <1..1688
                     /locus_tag="VFP93_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843672.1"
                     /translation="IKNQTAEETLLTLQLTEKLLRKILSIMSDQEIISDLGMAHHKMA
                     KWADLGYWFLEKLETLALKLKKVGNKSNIKKYLESTTIQEIKCHLKIVLLNVGFCLTD
                     AVTNDLELFFAEKYLEQAERINVCDLPHLNVNTFSKLVNPCGRAVIAYKLHDLYNFFS
                     YLDETTANYFRFHPAIELTKMTLYFSVLLRLSIIAKDTLEQKLPHLAVKYHNNIKKIF
                     ECAESDERFKKINIVGNENFLKDKAEFIKLADQATQNLQAKIRDALTNTFLSLKLRVF
                     AIQDSFAIFIPLEKLPNRKVFLHLPIRLKTLFAIDKIHDNDLVGNTKITGIRIILFSF
                     DINEIIPAISTFLELIEQASNNNKVETNVALASLNKLSFQENNNNVSTLETSAIMQEA
                     HVAQKSNIKTKIKKSKDSKSPIKGIPAPKVLETLHAHDFGFHTEDNPEVTPIYFSTQG
                     QKAQKPTVFVHWKGIPKVEEKIENLFKAMLNPTIEGLVTVGVRNQAGAKIFLSPQALG
                     DDWNLCGLRFKRKGNGAGWLRAIAIPEERVTVDLPDGKKQNRYLFTLGKAKNK"
     gene            complement(1820..>2023)
                     /locus_tag="VFP93_03335"
     CDS             complement(1820..>2023)
                     /locus_tag="VFP93_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843673.1"
                     /translation="LKHLQERGNTIVIIEHNLDVIKTADWIIDLGPEGGDKGGTIVTV
                     GTPEMVAQHKTSYTGEFLAPLLK"
BASE COUNT          712 a          365 c          351 g          595 t
ORIGIN      
        1 tcattaaaaa tcaaactgca gaagaaactt tgttaacttt acagttaacc gaaaaattgc
       61 tgcgaaagat cttatcaata atgagtgatc aagaaattat tagcgattta ggtatggcgc
      121 atcataaaat ggcgaaatgg gctgatctag gttattggtt tttggagaaa ttagagacat
      181 tggccttaaa gcttaaaaaa gtgggcaaca aaagtaatat taaaaagtac ctagaaagca
      241 caaccattca agaaatcaag tgtcatctta aaatagtttt actaaatgtg ggtttttgcc
      301 ttacagatgc tgtaaccaat gatttagagc ttttttttgc tgaaaaatat ctcgagcaag
      361 cagaacgcat aaacgtatgc gatttaccgc atttaaatgt aaatactttt tcaaaattag
      421 tgaatccttg tggtcgagct gtaatagcat ataagctcca tgatctctat aatttctttt
      481 catatttaga tgaaacaact gctaattatt ttcgttttca tccagccatc gaattaacaa
      541 aaatgacatt gtatttttcg gtcttgttga ggttatctat cattgccaaa gatacattgg
      601 agcagaagct tccacatctt gctgtcaaat accataacaa catcaagaaa atttttgagt
      661 gcgcggaaag cgacgaaaga ttcaaaaaga tcaatatcgt tggaaacgaa aactttttaa
      721 aagacaaagc ggaatttata aaattggcag atcaagcaac gcaaaattta caagcaaaaa
      781 tacgagacgc attaacaaat acttttctct cacttaaatt aagagttttt gcaattcaag
      841 atagttttgc aatttttatt cctttagaaa aactgccaaa ccgtaaagta tttttacatc
      901 ttcctatcag acttaaaaca ctatttgcaa ttgataaaat tcatgataat gaccttgtag
      961 gtaatacaaa aattaccggt attcgcatca tattattttc gtttgacata aatgagatta
     1021 tacctgcaat aagcacgttc ttagaattaa tagaacaagc ttcgaataat aataaggtag
     1081 aaaccaatgt tgccctcgca tccttaaaca aactttcttt tcaagaaaac aataataatg
     1141 tatcgactct tgagacatct gcgataatgc aggaagcaca tgttgcacaa aaatctaaca
     1201 taaaaactaa aattaaaaaa agtaaagatt ctaaaagccc tataaagggc attccggcac
     1261 ccaaagtgtt agaaacttta catgcgcatg attttggatt tcacacggaa gataatccgg
     1321 aagttacgcc aatttatttt tcgacgcaag gccagaaagc gcaaaaacct accgtttttg
     1381 tccactggaa gggtattcct aaagtagaag agaaaattga aaacttgttt aaagcaatgc
     1441 taaatccaac gatagaaggc ttagtcactg tgggggttag aaaccaagca ggtgctaaaa
     1501 tatttttgtc gccacaagcc ttaggagatg attggaacct ttgcgggctg cgattcaaaa
     1561 gaaaagggaa tggtgctggt tggttgcgtg caatagccat ccctgaagaa agagtcacag
     1621 tagatttacc cgacggtaag aaacaaaatc gttatctatt taccctaggt aaggccaaaa
     1681 ataagtagtg tacttggctc tgcgttatca gcgtcagcca tcaaccctct ttattacggc
     1741 gtgaatatac aatcttatct ggcgcgtgct cgtccgcgcc atccattgct atatcttaaa
     1801 tttataaagc acaacacaat tatttcaata aaggtgctag aaattccccc gtataagaag
     1861 ttttatgctg agcaaccatt tcaggagtac ccacggtaac aatggtgcca cctttatcac
     1921 caccttccgg tcctaaatca ataatccaat cggcggtttt tatcacatct aaattgtgtt
     1981 caatgattac aatcgtattg cctcgttctt gcaaatgttt taa
//
LOCUS       DASTIB010000194         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_432928, whole genome shotgun sequence.
ACCESSION   DASTIB010000194 DASTIB010000000
VERSION     DASTIB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432928"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="VFP93_03340"
     CDS             complement(<1..1701)
                     /locus_tag="VFP93_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843674.1"
                     /translation="MLNFELNIQQDSLEKIKVFFQKLRMLSKKLHQIQYPNSTEFLCA
                     INHICAALTNLNLEHLAQTQPNLENLLVNIKKQKLVLADLNKKIAQVEQQQAQSFFSK
                     FFGLFKEKDALLQVRHNMKQQLAQEIEKRETELSHQLLSIIFNETNQSLDKLFPFYVN
                     SIMAPLEQNQQLANSLDMNLLSILLLVKKQFFYFFTFAQTLQGTMQTVDLIKNVPYFA
                     WTSVDTNHRSYLKRGFDFFMEQSPISSATSAMADWFFLSFDAFLENFDFFYINMYSHK
                     MIPIELQKNVHTSLTEFYISQNFSFLYPLAPIIMEVVDKISEENEQIMPKDALELFSK
                     LGLLAFSPNNFTQPNTVIILQLYESIKTKLPDCYFKNSLEYKTKFFTIFIKFSKNILA
                     TLDHPLVKEIWDKKKVEQLANAIHVAKNMNLHHEADIQEGMKLLYRALYQLQHTHKEE
                     SNQLVPYVTNLLQYSIYELNTIFYSNSALEFNPNFPEAVVNILFELGIRKLNALINTN
                     MDLITKINHSMEEEKTIVYAQPFFQQTSQAVRNIDKILFLYSTGGSGHEQAMRAHVQI
                     E"
     gene            complement(1813..3264)
                     /locus_tag="VFP93_03345"
     CDS             complement(1813..3264)
                     /locus_tag="VFP93_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009670947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET9843675.1"
                     /translation="MTRIANIFRILGILVMLYSITLIPPILVEEWYKDGSLTPFVYST
                     LFLLFAGLLIWIPFRKYKTELLSRDGFIIVVAFWMVFAIVGAFPFYLTANPMLSFTDA
                     FFESVSGVTTTGSTILIGLDKLPHSLLYYRQQLQFLGGMSIIILAVAILPMLGVGGMQ
                     LYRAETTGPVKDNKLTPRIAQTAKAILFIYLIFTIVCAICYWLLGMGLFDAISYSFST
                     VSTGGFATHDLSMNYFYNPLLKLVAILFMFLGAVSFNLHFMVIHTQKIGIYRNDPELK
                     TFMWLLLIFFICVWVGFFLFQVPDESNIVLDSIFTVVSLCTTTGLIASDFSTWPAFIP
                     LMLLFGGIIGGCAGSTTGGLKVIRFLLLMKQGNREINRLIHPHAHYTIKLGESPVSNK
                     VIEGIWGFLAMYFGIFCLFLLLLLLTETDLLTAYTALIASISNSGIGLGSVSHNFAQL
                     SDLSKWILSFAMIVGRLEIFTILVLFSPEFWRN"
     gene            complement(3270..>3892)
                     /gene="trkA"
                     /locus_tag="VFP93_03350"
     CDS             complement(3270..>3892)
                     /gene="trkA"
                     /locus_tag="VFP93_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET9843676.1"
                     /translation="DSLYVKIIDHNRKRIELLSEKLENSVVLLGDAANKDLLMDENID
                     NTDVFCAVTNRDEANIMSAILAKRLGAKKVMALINSTAYVDVVEGGEIDIAFSPQQAT
                     IGSLLTRIRRGDIVNVYSLRRGAAEAIEAIAHGDPRSSAVVGKTIQEVALPPGSIIGA
                     IVREDRVLIAHENLLIESNDHIILFVVDKKRIPQVERLFQVGITFL"
BASE COUNT         1327 a          690 c          682 g         1193 t
ORIGIN      
        1 ttctatttgc acgtgtgcac gcatagcctg ttcatgccca ctgccacccg tagaataaag
       61 aaatagaatt ttgtcaatgt tgcgaacagc ttgtgaggtt tgttggaaaa aaggctgcgc
      121 gtatacaatc gttttctcct cttccatgct atggttaatt ttggttatta aatccatatt
      181 cgtatttatt aaggcattta attttcttat acctaactca aataatatat taacgacggc
      241 ttctggaaaa ttaggattga attctaaagc actatttgaa taaaaaatag tattcaattc
      301 ataaatgcta tattgaagca ggtttgttac ataaggtact agctgattag attcttcttt
      361 atgggtatgt tgtaattgat aaagtgctct atatagtagc ttcataccct cttggatatc
      421 ggcttcatga tgtaaattca tgttttttgc aacatgaatg gcattggcta attgctcaac
      481 ttttttctta tcccagattt cctttactaa tggatgatct agagtagcta aaatattctt
      541 tgagaatttg ataaaaatag taaaaaattt tgttttgtat tccagactat ttttgaagta
      601 gcaatcgggt aatttagtct tgatcgattc gtatagctgc agaataatta ctgtattcgg
      661 ttgcgtaaaa ttgttgggag aaaatgctag caatcctaat ttgctaaata attcaagagc
      721 atccttcggc attatttgtt cattttcttc agaaatttta tccactactt ccatgataat
      781 gggcgctaga ggatacaaaa aactaaaatt ctggctaata taaaattctg ttaatgacgt
      841 atgaacgttc ttttgtaact cgataggaat cattttgtgt gaatacatat taatgtaaaa
      901 aaagtcaaaa ttttcaagaa aagcatcaaa acttaagaaa aaccaatcag ccatagcgct
      961 ggttgcactg gaaatcgggg attgctccat aaaaaaatca aacccgcgtt taagatagga
     1021 ccgatgattt gtatctaccg aagtccaagc aaaataagga acatttttaa tcaaatctac
     1081 ggtttgcatc gtaccttgca aagtctgggc aaaagtaaaa aaataaaaaa attgtttttt
     1141 tactagcaat aaaatactta aaagattcat gtctaagcta ttagcgagtt gctgattttg
     1201 ttccaagggc gccataatac tattaacata aaagggaaat aatttatcaa gactttgatt
     1261 tgtttcattg aaaattatcg ataagagctg gtgtgataat tccgtttcgc gtttttctat
     1321 ttcttgcgct aattgttgct tcatattgtg acgtacctgc aatagtgcat ctttttcttt
     1381 aaacaatccg aagaacttgg agaaaaaaga ctgtgcttgt tgttgttcaa cttgagcaat
     1441 cttcttattg agatcagcca aaacaagctt ttgcttttta atattaacaa gtaaattttc
     1501 caaattgggt tgtgtttgcg cgagatgctc taagtttaaa ttagttaaag cggcacaaat
     1561 gtggttaata gcacacaaaa attcagtgct attaggatat tggatttggt gtaatttctt
     1621 agaaagcatt cgtagcttct gaaaaaaaac cttaattttt tcaagagaat cttgttgaat
     1681 atttagctca aagtttaaca taaagccatc agcattttga agaatggctt atagtaaggt
     1741 tttatttaat atttgataga aaaagaagat agattgcgaa tataatagta caaggggtat
     1801 taattaagga ttttaattac gccagaattc tggtgaaaac aaaacgagga tggtaaatat
     1861 ttccaagcgt ccaactatca tggcaaagct taaaatccat ttgcttaaat cgctcaattg
     1921 cgcaaaatta tgggaaacgg aaccaagccc tatgcccgaa ttagagatac tagcaatcag
     1981 ggcagtgtaa gcggtaagca aatccgtttc ggtgagtaac aacaataata aaaaaaggca
     2041 gaaaatccca aaatacattg ccaagaaacc ccaaatgcct tcgatgactt tattcgatac
     2101 tggcgattct cctaatttaa tggtgtaatg cgcgtgggga tgaattaatc gattgatttc
     2161 acgatttcct tgtttcatca ataataaaaa tcgtattact ttcagcccac cggtagtaga
     2221 tcctgcacac cctccaataa tgccgccaaa taacaacata agcggaataa aagcaggcca
     2281 tgtcgaaaaa tctgaagcaa taagacctgt tgtggtgcaa agtgatacca ccgtaaatat
     2341 actatctaaa acgatgttgc tttcgtctgg aacttgaaat agaaaaaacc ccacccagac
     2401 acaaataaaa aaaatcaata aaagccacat aaacgttttg agctcgggat catttctata
     2461 gatcccaatt ttttgcgtat ggattaccat aaaatgtaag ttaaagctta ctgcgcctaa
     2521 aaacataaag aggattgcga ccaactttaa taacgggtta taaaaataat tcatgcttaa
     2581 atcgtgggtg gcaaaaccac cggtagatac cgtagaaaaa ctataactga tggcatcaaa
     2641 aaggcccatt cccagcaacc aatagcaaat agcgcataca atggtaaaaa ttaaatatat
     2701 gaacaaaatt gcttttgcgg tttgggcaat tcttggagtc agtttattat cttttacagg
     2761 gcctgtagtc tcagcgcgat agagctgcat accccctacc cctaacatag gaagaatggc
     2821 aactgctaaa ataataatac tcatcccgcc taaaaattgt aattgttgcc gatagtacaa
     2881 tagagaatgt ggcagtttat ctagtccaat taagatcgta gagcctgtgg ttgtgacacc
     2941 agataccgat tcaaaaaaag catcggtaaa agataacatt ggatttgctg ttaagtaaaa
     3001 cggaaaagca cccacaatgg cgaaaaccat ccaaaatgca actacaatga tgaatccatc
     3061 tcgagaaagc aattcggttt tgtattttct aaaaggaatc caaattaaaa ggcctgcaaa
     3121 tagtaaaaat aacgtagaat aaacaaaggg tgttaaacta ccatctttat accattcttc
     3181 tacgagaatg gggggaataa gtgtaatgct ataaagcatt accaaaatac ctaagattct
     3241 aaaaatattt gcaatacgtg tcatagttct tataaaaaag taataccaac ctgaaataat
     3301 cgttccactt gtggaattct ctttttgtct acaacgaata aaataatatg atcattcgat
     3361 tcaattaata aattttcgtg tgctatcaaa accctgtctt cacgtacaat ggcaccaatg
     3421 atagaaccgg gtggtaaagc aacttcttga attgttttac ccaccacagc agatgatctt
     3481 ggatccccat gcgctatggc ttcaatcgcc tcagctgccc ctcgtcttaa cgagtaaaca
     3541 ttgacaatgt caccacggcg aattcgtgtc agcaaacttc cgatggttgc ttgttgtggt
     3601 gagaaagcaa tatcaatttc accgccttcg acaacatcca cataggccgt actattaata
     3661 agtgccatga cttttttggc acctaaacgc tttgccaaaa tagccgacat aatattagct
     3721 tcatcacgat tcgtcacagc acaaaaaaca tcggtgttat caatattctc atccatgagt
     3781 aaatctttat tggcagcatc tcctaacagc acgacgctat tttctaattt ttcggataat
     3841 aactcgatgc gttttcgatt gtgatctatg atctttacat agagggaatc tt
//
LOCUS       DASTIB010000195         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_865076, whole genome shotgun sequence.
ACCESSION   DASTIB010000195 DASTIB010000000
VERSION     DASTIB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865076"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFP93_03355"
     CDS             complement(<1..496)
                     /locus_tag="VFP93_03355"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9843677.1"
                     /translation="MRILIKQGRLIDPTQNLNQIGDLYIVHGKIVSVDNAPDGFQPDH
                     ILLAHNHFVIPGLVDLYAHLNDSYGAVQLHEAAVAAKRGFTTLFCAPDGKMIRDSITR
                     VEKFLESDVSYIDVLPIGALSEHLKGNAMSDLTALHAAGCCVFSNGIYPMQDLNFIRN
                     LYLYA"
     gene            complement(462..1472)
                     /locus_tag="VFP93_03360"
     CDS             complement(462..1472)
                     /locus_tag="VFP93_03360"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9843678.1"
                     /translation="MLNRSSASMQLDKAGNLKHLLTLEGLPAEVLHTILNKADTFFDK
                     SAKVIFQKEYLKDHTVVNLFFEASTRTRSTFELAAKKLGANVLNFNVATSATSKGESL
                     ADTVKNLMAMQCTLFVVRHSASGAAQFVAQQVSDDISVINAGDGCHAHPTQALLDIYT
                     IRKHRGDLSHLKVAIVGDVLHSRVARSQIHALQTLNTLEIRVIAPKTLLPRAIHKLNV
                     KIFHDLKQGLQDVDVIIMLRLQTERMLDNLLPTGNAFYREYGLTTHALNFAKKDALII
                     HPGPINRGVEIESEIADSPNAVILDQVTNGIAIRMAVMTLLANNQERVKYENPYQTRE
                     IN"
     gene            complement(1462..1896)
                     /gene="ruvX"
                     /locus_tag="VFP93_03365"
     CDS             complement(1462..1896)
                     /gene="ruvX"
                     /locus_tag="VFP93_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET9843679.1"
                     /translation="MSGPTKANSGIFLGFDFGVKKIGVSTGQSITRSASPLTTILNHN
                     DNINWHEITKTIQNWKPQACIVGLPLHADQSESITSQKAKDFGEELSNRFTIPVYYVD
                     EHLTTHSANVLCKDPLYKKHSRDAIAAMLILDTWLKGNFHVK"
     gene            complement(1893..>2421)
                     /locus_tag="VFP93_03370"
     CDS             complement(1893..>2421)
                     /locus_tag="VFP93_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET9843680.1"
                     /translation="AMPSLLDPNFSRAVAYICEHTPQGAMGIIINFPLSIHLGDILKN
                     MDIRTDLATINNLAILAGGPIQQERGFVIHKTSSKNWESSLELDDNISITTSKDILKA
                     MAQNKGPKEALVALGYSGWTAGQLEKEIAENSWLISPASLEVMFIVPHEQRWRAAAGL
                     VGVNVDNLSSEVGHA"
BASE COUNT          708 a          493 c          479 g          741 t
ORIGIN      
        1 ccgcatacag atatagattg cgaataaaat ttaaatcttg catcggataa atgccattac
       61 tgaaaacaca gcagccagcg gcatgtagag ccgttagatc tgacatcgcg ttgcctttta
      121 aatgttcgct taacgcacct attggtaaaa catcgatata cgatacatcc gattctaaaa
      181 acttttccac tcgggtaata ctgtcacgta tcatctttcc gtcaggtgca caaaataggg
      241 ttgtaaaacc gcgttttgca gcaacagctg cctcatgcaa ctgcacagct ccataagaat
      301 cgttcaaatg cgcatataaa tctaccaagc caggaattac gaaatgatta tgtgctaata
      361 ggatgtgatc gggttgaaaa ccatcaggag cattgtccac actaacaatt ttgccatgaa
      421 cgatataaag atcgccaatt tgatttaaat tttgcgtggg atcaattaat ctcccttgtt
      481 tgataaggat tctcatactt aactctttcc tggttgtttg caagcagcgt cattaccgcc
      541 atacgtatag caataccgtt ggttacttga tctaaaataa ccgcattggg actatctgct
      601 atctccgatt caatttcaac gcctcggttg ataggccctg gatgaataat taaagcatct
      661 ttttttgcaa agttaagggc atgcgttgtt aatccatatt ctcgataaaa cgcattgccc
      721 gtaggtaaaa gattatccag catacgctcg gtctgcagtc ttaacataat aattacatcc
      781 acatcctgta aaccttgttt taaatcgtga aatattttga cattcagttt gtggatagct
      841 ctagggagca atgttttagg agcaatgacg cgaatttcta acgtatttaa ggtttgcaaa
      901 gcatgtattt gcgaacgggc tacccgagaa tgtaaaacat cgccaacaat agcaaccttt
      961 aaatgggaca agtcgccccg atgcttgcgt atagtataaa tgtctaagag ggcttgtgtt
     1021 ggatgcgcgt gacaaccatc tcccgcatta attacggaaa tatcgtcaga tacttgttgt
     1081 gccacaaatt gcgcagcgcc cgacgcacta tggcgcacaa caaacagtgt acactgcatc
     1141 gccattaaat tttttacggt atctgctaaa ctttctcctt tggaagtagc agaagttgcc
     1201 acgttaaaat tcaacacgtt cgcacctaat ttttttgcag ctaactcaaa agttgagcgg
     1261 gttctcgtgc ttgcctcgaa gaataaatta actacggtat gatcttttaa atattctttt
     1321 tgaaaaataa cttttgcact tttgtcaaaa aaagtgtctg ctttgttgag gatggtatgt
     1381 aagacctcgg ctggcaatcc ttctaaagtt agaagatgtt ttaaattgcc tgctttatct
     1441 aattgcatgg aagcagacga tctatttaac atgaaaattt ccttttaacc aagtatctaa
     1501 aattaacatg gcggcaatgg catcgcggga gtgtttttta tacaagggat ctttgcataa
     1561 aacatttgca gaatgtgttg ttaaatgctc atctacataa taaacaggga tcgtaaatcg
     1621 attgcttaac tcctccccaa aatcttttgc tttttgcgat gttatagatt cgctttgatc
     1681 tgcgtgtaag ggcaatccaa caatgcaagc ttgcggcttc caattttgaa ttgtttttgt
     1741 tatctcatgc cagttaatat tgtcgttatg atttaagata gtggtaagag ggctggctga
     1801 gcgtgtgatg gattggccgg tactaacacc aattttttta acgccaaagt caaatcctag
     1861 aaaaataccg gaatttgctt ttgtgggccc actcatgcat gaccgacttc agaggataga
     1921 ttatctacat taactcccac caaacctgcc gcggctctcc agcgttgttc atgtggaact
     1981 ataaacatga cctctaaaga ggcaggactt attaaccaag aattttccgc aatttctttt
     2041 tctaattgac ccgcagtcca gcccgaatag ccgagagcca caagtgcttc tttaggtcct
     2101 ttgttttggg ccatagcttt taagatatct ttagaagtgg taatagatat gttgtcgtct
     2161 aactctaaag aagattccca gtttttagaa gaggttttat ggataacaaa gcctcgttct
     2221 tgctggatcg gaccaccggc gagtatcgca aggttattta tagtggctaa atcggtgcgt
     2281 atatccatgt tttttaaaat atcacctaaa tgaatgctta aaggaaagtt aataatgatt
     2341 cccattgctc cttgaggcgt gtgctcacaa atataagcca ccgcgcgcga aaaattagga
     2401 tccaataggc ttggcatggc a
//
LOCUS       DASTIB010000196         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1300706, whole genome shotgun sequence.
ACCESSION   DASTIB010000196 DASTIB010000000
VERSION     DASTIB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300706"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="VFP93_03375"
     CDS             complement(<1..2285)
                     /locus_tag="VFP93_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9843681.1"
                     /translation="MLHLVQEAHTALKNKAFDKAAEKAHKAIKSDPLISSAWHILGLV
                     ALEQKKYQEAVGFLQKAIQAEPKALYYFNLGIAFKRLNQLEDSIKSYLQCLKMDPKMI
                     RALSNLANVYRANQQYDNALECYQRTLDLDPTNAIAFYNLGILYQSKEAFEDAIKAYT
                     KAIKYNPNIAKAFCNLATIYSKLRRYEKSIQFYERAIKIDRNFFDAYNNLGGTLIELG
                     KFQDAEIQLRKALQLEPASCATLRNLTLCKEYKNPQDKDAKSILKLLKNPKLTSDEVT
                     HLHFALGKIYHDCLLYDKAFFHYQKGNDLHFAKFPFNIFSLQDYTRDIVQLFDEATAV
                     AQKNLTQTKIQPLIILGLPRSGKSVLENILKHHPELCGAGEVGLAEFATLWEKEQQEA
                     FPHIIKKLDAANAQKIVDQYERKILRDLTPKKYVIDTMPGNVFYLGLFLMLFPNARVI
                     SIKRDRNDLGLFIYFKYFVNRHHYAYHLKTIGQYYKLYQYLVRHWVNLYRDKILEVRY
                     EDLILHNESTLERMAQFLKIDANAFSFKEPLSSHEIGQHHNFKAYLEPLQQGFQFDPQ
                     NDEIRLKEALIEATEKHKNKKYEEAAKLYKYILQYDPNNPVPIHLLGVLAHEMGHDER
                     AIALIKKAIEMNPNYAQAHNNLGVILLKNKRQIEAENHFKIAETIVEAANTEKFKVLT
                     PEHSIVLQDAFLKNYQKIKEHERKILYKGDVKPTTTSFMTKSWDYYFKDLSYGSYRLG
                     GTTENIIWRMRTWHFLFKNLA"
BASE COUNT          734 a          437 c          430 g          889 t
ORIGIN      
        1 atagcaagat ttttaaataa aaaatgccac gtacgcatgc gccatataat gttctctgtc
       61 gtacccccta aacgataact accatagctt aaatctttaa aataataatc ccaggatttt
      121 gtcatgaaag aagttgtggt aggctttaca tcgcctttat agagaatttt acgttcatgt
      181 tcttttattt tttgataatt ttttaaaaat gcgtcttgta agacgataga atgctctggc
      241 gtcaacactt taaacttttc ggtgttggcc gcctcaacga tagtctctgc aatcttaaaa
      301 tggttttccg cttcaatttg acgtttattt tttaataaaa taacccctaa gttgttatgt
      361 gcttgcgcat aatttggatt catttctatt gcttttttta ttaatgcaat ggctctttca
      421 tcgtgtccca tttcatgcgc aagcacgcct aataaatgaa tcggaacggg gttattagga
      481 tcatattgca gaatatattt atatagtttt gctgcttctt cgtatttttt atttttatgt
      541 ttttcggttg cctctataag tgcttctttt aaacggattt catcgttttg cggatcaaat
      601 tgaaaacctt gttgtaaagg ttctaaatat gctttaaaat tatggtgttg tcctatttca
      661 tgcgaagata aaggttcttt gaaggaaaaa gcattcgcat ctatttttaa aaattgcgcc
      721 atacgttcca acgtactttc gttatgaaga attaaatctt cataccgaac ttctaaaatt
      781 ttatcccgat aaagatttac ccaatgacgt accaaatatt gatataattt gtaatattgc
      841 ccaatagttt ttaaatgata agcataatga tgacgattca cgaagtattt aaaatagata
      901 aacaacccca gatcgtttct atcacgtttt attgaaatga ctcttgcatt gggaaataac
      961 ataagaaata atcccaaata aaatacgttt cctggcatcg tatcgattac atattttttg
     1021 ggagttaaat cgcgcaagat cttgcgttca tattgatcta ctattttttg cgcatttgca
     1081 gcgtctaatt tttttataat atgcggaaac gcctcctgtt gctctttttc ccataaggtg
     1141 gcaaactcgg caagtcctac ttctccggct ccacataatt ccggatgatg ctttaaaata
     1201 ttttccagca cactttttcc agatcggggc aaacctaaaa taatcagcgg ttgtattttt
     1261 gtttgcgtta agtttttttg cgcaaccgct gtcgcttcat caaataattg aacaatatcg
     1321 cgcgtataat cttgcaaact aaaaatattg aaaggaaatt tagcaaaatg taaatcgttg
     1381 cccttttgat aatgaaaaaa tgctttatcg tagagtaagc aatcatggta tattttgcct
     1441 aatgcaaaat gcaagtgcgt cacttcatcg ctggttaatt ttggattttt taataacttt
     1501 aaaatggatt ttgcatcttt atcttgtgga tttttgtatt ctttgcacaa tgttagattg
     1561 cgcaacgttg cacacgatgc aggttctaac tgaagggctt tgcgtaattg tatctctgca
     1621 tcctgaaatt tcccaagctc gattaacgtg ccacctaaat tgttataagc gtcaaaaaaa
     1681 tttcgatcta tttttatggc tctttcgtaa aattgtatac ttttttcata acgacgcaat
     1741 ttagaataga tcgttgctaa attgcaaaaa gcttttgcaa tattagggtt atatttgata
     1801 gctttggtgt aagcttttat ggcatcctcg aaggcttcct tcgattggta taaaatgcct
     1861 aaattataaa atgcaattgc attggtagga tcaagatcta gggtgcgttg ataacactct
     1921 aaagcattat catattgttg atttgcgcga taaacatttg ccaaattgct taatgcgcgg
     1981 atcattttgg gatccatttt taaacattgc aaatagcttt ttattgaatc ttctaattga
     2041 ttcaaccttt tgaaagcaat acctaagtta aaataataca aagccttagg ctctgcttga
     2101 atggctttct gtaaaaaacc gaccgcttct tggtattttt tttgctcaag agctaccaac
     2161 cctaaaatat gccatgcact actgattaat ggatcagact tgatcgcctt atgggctttt
     2221 tccgcggctt tgtcaaaagc tttatttttt aaagcagtgt gcgcttcctg tacaagatgc
     2281 agcatacctt tcgtttagta cccattgcct aactctggaa taagtatagg cacgggcgcg
     2341 taagaaaggc ttaaagactt ggcaaagaag cccgtctttg tgtggataat tgcggtattt
     2401 tttcttctct gaatggacta tagcgattaa gtggtaaatc aacgtgtttc aaaagttgtg
     2461 aaaacggaat agcagaaaat tcaaaatcag
//
LOCUS       DASTIB010000197         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_60802, whole genome shotgun sequence.
ACCESSION   DASTIB010000197 DASTIB010000000
VERSION     DASTIB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60802"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VFP93_03380"
     CDS             complement(<1..745)
                     /locus_tag="VFP93_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843682.1"
                     /translation="MLGWYTLQTLKIDTNTQNMISEALDWRQDYIRYKKIFSNNHQTI
                     VVVIEGDNFEATKIASKELYNILRKDPQFISVFSFSLSEFLAKNHLLYLSLEQLADLK
                     KEALQVAPLLSFTREDLGSFLNFLQYSTTNSQSVQRILPILKEFKNLLHPNQDEHMHF
                     ASFAEYFKIANNKSFIEILEVKPKLSYDTLFPGETVVNAIQRYFEVLKLSKKYQVSLK
                     MTGEVPLAYEELKGVSYGTGWATLFAALLV"
     gene            complement(854..1555)
                     /locus_tag="VFP93_03385"
     CDS             complement(854..1555)
                     /locus_tag="VFP93_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843683.1"
                     /translation="MQKIGILVSSPLEGRSFFKDLMYQEPYAFSKNIWVICAGRGDKN
                     QKKATTKLIFKGIDGIISWGFAGGLDPTLASGTIILPQQIIHPFGPTLDMDKKWHKKL
                     KNQLEKHFPVITHPLAYAQHILHTPKQKRNLFERTKAAAVDQESFIIGDMVKEAKLPF
                     ISLRVILDDAYTALPKNIQTLQDNTGTLIKKNIFLELLKPHLWLNYLTLSRQYSKTNQ
                     ILHHVIDASFNHTFN"
     gene            complement(1558..>2531)
                     /gene="shc"
                     /locus_tag="VFP93_03390"
     CDS             complement(1558..>2531)
                     /gene="shc"
                     /locus_tag="VFP93_03390"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET9843684.1"
                     /translation="FSPVWDTSLALLALQEESLGQITTTHYEACQWLKSRQLTNEKGD
                     WQVNRPTLLGGGFAFQYENSYYPDLDDTAVVAIALFQGNDHNLYDDVISRAANWLAGM
                     QSQGGGFASFDVDNTHYYLNEIPFADHGALLDPPTADVSARCAMLFGVLQQNSYHPNL
                     ISCIDYLLKSQEKNGSWFGRWGTNYIYGTWSVLVALEKVGFPANHPAIRKAVQWLKSI
                     QNPDGGFGESNASYFHSGGEVVPHPSTSFQTAWAILGLLAAGEEHSNTVQRGVQYLVD
                     TQKKNGFWHEPYFTAPGFPRVFYLKYHGYSKYFPLWALARFRNKIQR"
BASE COUNT          804 a          482 c          442 g          803 t
ORIGIN      
        1 atacaagcaa ggctgcaaat agggtagccc aacccgttcc atagcttaca ccttttagct
       61 cctcataagc taaaggaact tcccctgtca tttttaaact aacttgatat tttttactta
      121 atttaagtac ctcaaaatat cgctgaattg cattaactac agtctctcct ggaaataaag
      181 tatcataaga caatttaggc tttacctcta aaatttctat aaacgattta ttgtttgcaa
      241 tcttaaaata ttcagcaaaa ctggcgaaat gcatgtgttc atcttgatta ggatgtaata
      301 gatttttaaa ttctttcaaa ataggtaaaa ttctttgtac ggattgagaa ttagttgtag
      361 agtattgcaa aaaatttaaa aacgaaccta aatcttctcg cgtaaaagat aaaagaggag
      421 caacctgtaa tgcttctttt ttcaagtcag ccaattgttc taatgataaa taaagtaagt
      481 ggttttttgc aagaaattca cttaaagaaa aagaaaatac ggaaataaat tgcggatctt
      541 tacgtaaaat attatagagt tctttgctcg cgattttcgt cgcctcaaaa ttatcgcctt
      601 cgatcacgac aacaatagtt tggtggttat ttgaaaatat ttttttatag cgaatataat
      661 cttgtcgcca atctaatgcc tcggatatca tattctgtgt attcgtatcg atttttagcg
      721 tttgcagcgt ataccaacct aacaaaaaac taatgcttgc aaaaaaacaa acgattattt
      781 tcggataggt gcaaacgcaa tgtagccatt tgacaaaaag ggcagagcat cgattggata
      841 aaaaaaaacg catttaatta aacgtatgat tgaaggaagc gtcaatgaca tgatgtaaaa
      901 tttggttggt ttttgaatat tgtcgactga gtgttaaata atttaaccat aaatgtggtt
      961 ttagtaattc tagaaaaata ttttttttga taagggttcc cgtgttatct tgcagcgttt
     1021 gaatattttt tggcaatgct gtgtaggcat catctaaaat gacacgtaag ctaataaaag
     1081 ggagcttagc ttcttttacc atgtccccaa taataaagct ttcttgatct acagctgcag
     1141 ccttagttct ttcaaataaa ttccgttttt gctttggcgt gtgtaaaata tgctgggcat
     1201 aagctaatgg gtgggtaata acaggaaaat gtttttctaa ttgatttttt aattttttat
     1261 gccatttttt gtccatgtca agtgtcggac caaaaggatg aataatttgt tgtggtaaaa
     1321 taattgttcc actcgctagg gtaggatcca accccccggc aaaaccccaa ctaattatgc
     1381 catctatccc tttaaatatg agtttagttg ttgctttctt ttgattttta tccccgcgtc
     1441 cagcacaaat aacccaaata tttttggaaa acgcataagg ctcttgatac attaaatctt
     1501 tgaaaaaact gcgcccttcc aacggtgaac ttactagaat tcctatcttc tgcatactta
     1561 cctctgtatt ttattgcgaa agcgcgctaa agcccataag ggaaagtatt tagaataacc
     1621 atgatatttt aaataaaaaa cgcgtggaaa tcccggggcc gtaaaatagg gctcatgcca
     1681 aaaaccattt tttttctgcg tatctactaa atattgaact cctctttgca cggtattaga
     1741 atgctcttct cctgcggcca ataaccccaa aattgcccaa gccgtttgaa aagaggtgct
     1801 tggatgcgga acaacttcgc caccactgtg aaaataggat gcgttacttt cgccaaaacc
     1861 accatctgga ttttgtatgc ttttcaacca ttgtaccgct ttacgtatag ctggatgatt
     1921 ggcgggaaat cctacttttt ctaatgctac caataccgac cacgtaccgt aaatataatt
     1981 ggtgccccaa cgcccaaacc aagaaccatt tttttcttgc gattttaata aataatcgat
     2041 acaagaaatt aaattcgggt ggtaactatt ttgctgtaag acaccaaata acattgcgca
     2101 ccgcgcgcta acgtcagcgg taggcggatc taacaaagcg ccatgatccg caaaaggaat
     2161 ttcatttaaa taataatggg tattatccac atcaaaggat gcaaatccgc ctccttggct
     2221 ttgcatccct gccagccaat tcgccgcacg tgagatgaca tcatcgtata aattatgatc
     2281 attaccttga aaaagggcaa ttgcgactac agcagtatcg tccaaatctg gataataaga
     2341 attttcatac tgaaatgcaa aaccaccgcc caacaaggtt ggtctattta cttgccaatc
     2401 gcctttttca ttggtcaatt gtctgctttt aagccattgg catgcttcat aatgtgttgt
     2461 ggtaatttgt cctaaagatt cttcttgtaa tgccaataag gctagagacg tgtcccaaac
     2521 aggagaaaaa c
//
LOCUS       DASTIB010000198         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1017109, whole genome shotgun sequence.
ACCESSION   DASTIB010000198 DASTIB010000000
VERSION     DASTIB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017109"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="maeA"
                     /locus_tag="VFP93_03395"
     CDS             complement(<1..706)
                     /gene="maeA"
                     /locus_tag="VFP93_03395"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HET9843685.1"
                     /translation="MLKYIRHLDRDTGHEWIETELTGKALLNHSLLNKGTAFSKEERE
                     SLGLLGKLPFEVESLDQQVLRARYQYMRYPSVLQKHIYLNNLHDKNEILFYKLCDEDL
                     ESIFPMIYTPGVGSAVKEFSHEFRQPRGIYISYPDRDHMETILDNRTHPEVDLLVVTD
                     GEGVLGIGDQGIGGMDIPIAKLIVYTLCGVDPYKTLPIMLDVGTNNQSLLNDPMYLGW
                     RHPRIEGETYDTFIEQF"
     gene            complement(732..>3096)
                     /gene="purL"
                     /locus_tag="VFP93_03400"
                     /gene_synonym="purI"
     CDS             complement(732..>3096)
                     /gene="purL"
                     /locus_tag="VFP93_03400"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET9843686.1"
                     /translation="GGLSNAVIELIAQGGVGAHIDLRQIPNAEPSMTPLEIWCNEAQE
                     RYVLAIEEDKLGIFDKVAKRERCPYAVIGTTTSSNILEVYDPLFNAYPVQVPISLMLE
                     GSPKLLKIVDTYATKKLASKVTMDLKEALEQIIQFPAVADKTFLITIADRSVGGLVHR
                     DQMVGPWQVPVADCAITLRDFQSDQGEAMAMGERSPLGVLNPKASVRMSIAEALTNIL
                     AADIEKLSDIKLSCNWMAACNDPEEDNFLFHAVDAVGKEMCPQLGIAIPVGKDSLSMR
                     MRCGPTNQEEVKAPLSLIVSAFAPVKNIHASLTPELSEYAHTLWFLDLARGQKRLGSS
                     CLQQMQNNFGDETPDVDDVSFFKNSLLALIELKNAKVVLAYHDRSDGGLWTSLLEMAF
                     ASKKGLTIDLTSFMLNKKEAMRFLLNEELGVVLQIDEKHSYQFEEIISKYKMKAWCKP
                     IASIHDSSNISVVFQQETLYENSRTALHVLWSKVSFELQKIRDNERCALEAYAIIEAE
                     SDPGLQATHVSFSPIEPMFRTKPKVAILREQGVNGQREMAHAFMTAGFEAFDVTMTDL
                     LTERFNLNNFQVLAACGGFSYGDVLGAGLGWANAILFHEKLLEMFVNFFNRSDTLTLG
                     VCNGCQMLSFLKNIIPGASCWPKFVQNQSQQFEARLSLVEISRSSSLWFRTMEGSRLP
                     IVVSHGEGKVYFERENHLQHLQTNELINLQFIDPNNLLPTEYYPYNPNGSQCGITAIS
                     SEDGRATIMMPHPERVVRVAQMSWAPKHWQDKSPWQQLFYNAYWVLK"
BASE COUNT          912 a          666 c          568 g          950 t
ORIGIN      
        1 caaattgctc aatgaacgta tcatacgttt cgccctcgat gcgcggatgg cgccaaccta
       61 aatacatagg atcatttaat aaactttggt tattggtacc cacatcgagc ataataggta
      121 atgttttgta aggatcaacg ccgcataacg tataaacaat caattttgca ataggaatat
      181 ccatcccacc aattccttga tcgccaatac ccaatacccc ttcgccgtcc gttaccacca
      241 ataaatcgac ttctgggtgg gttcgattat ctagaatggt ttccatgtgg tctcgatcag
      301 gataagatat atatataccg cgtggttggc gaaactcatg actaaactct tttacggcag
      361 aacctacccc gggtgtataa atcatgggaa aaatagactc taaatcctca tcacacaatt
      421 tataaaataa aatttcgttt ttatcatgca aattatttaa ataaatatgt ttttgcagaa
      481 cactgggata acgcatatat tgatagcgtg cgcgcagaac ttgttgatct aagctttcaa
      541 cttcaaaagg taatttacct agtaaaccta aactttctcg ctcttctttg gaaaacgcag
      601 tacccttgtt taaaagagaa tgatttagca aagctttgcc tgtcaattcg gtttcaatcc
      661 actcatgacc tgtatctcta tccaggtgac gaatgtactt taacatttgc ggatccttga
      721 tttttcttca gttattttag tacccaatat gcattataaa agagttgttg ccacggactt
      781 ttatcttgcc aatgtttcgg tgcccatgac atttgtgcta cccgtactac tctttctgga
      841 tgtggcatca taattgtagc acgtccatct tcgctggata tggccgtaat tccacattgt
      901 gacccattag gattgtaagg ataatattct gtaggtaata aattgttggg atcaataaat
      961 tgtaaattta ttaactcgtt agtttggaga tgttgcaaat ggttttcacg ttcaaaatat
     1021 actttacctt caccgtgtga aaccacgatg ggtaaacgac taccttccat agttcgaaac
     1081 caaagggagg atgaacggga aatttcgact aaacttaacc tcgcttcaaa ttgttgcgat
     1141 tgattctgca cgaatttcgg ccaacaggaa gcgccgggaa ttatattttt aagaaaggac
     1201 aacatttgac aaccattaca tacgcctaag gttaacgtat cgcttcgatt aaaaaagtta
     1261 acaaacatct ctaacaactt ttcatggaaa agtattgcgt ttgcccaacc aagacctgct
     1321 cccaaaacat ctccataaga aaaaccacca catgccgcta acacctgaaa attgtttaaa
     1381 ttaaatcgtt cggtaagcag atcggtcatc gtaacatcaa aagcttcaaa gcctgccgtc
     1441 ataaaagcat gtgccatttc acgttgtcca tttacgcctt gttctcttaa tatggcaact
     1501 ttgggttttg ttctaaacat gggctcaata ggcgaaaagc taacatgcgt tgcttgtaag
     1561 ccaggatcag attctgcttc gattattgca taggcctcaa gggcacaacg ttcattgtcg
     1621 cgtatttttt gcaattcaaa acttactttt gaccataaaa catgcaaagc tgtacgcgaa
     1681 ttctcataga gggtttcttg ttgaaaaaca acagaaatgt tcgaactatc gtgaatactt
     1741 gcaataggtt tacaccatgc tttcatttta tatttggata ttatttcttc aaattgatag
     1801 gagtgctttt cgtcaatttg caaaactaca cccagttctt cattaagaag aaaacgcatt
     1861 gcctcttttt tattcaacat aaaagaagtt agatcgatag ttaatccttt cttcgaagca
     1921 aaagccatct ctaataagct cgtccacaaa cctccatcgc ttcgatcgtg ataagctaaa
     1981 actacttttg cgtttttaag ctctattaag gcaagcaaag aatttttaaa aaagctaaca
     2041 tcatcgacat caggcgtttc gtctccaaaa ttattctgca tttgttgtaa acaactgctt
     2101 cctaaacgtt tttgacctcg cgcaagatcc aagaaccata aagtatgcgc atattcggaa
     2161 agttcgggtg ttaagctggc atgaatattt tttacggggg caaaggcaga aactattaaa
     2221 gaaagaggtg cttttacttc ttcctggtta gtaggaccgc atcgcatccg catagataaa
     2281 gaatctttac ctaccggtat agctatgcca agttgcggac acatttcttt acccacggca
     2341 tctaccgcat gaaatagaaa attgtcttct tcagggtcgt tgcatgcagc catccaatta
     2401 catgataatt ttatatccga taatttttca atatctgctg cgagaatatt ggtaagtgct
     2461 tcggctatgc tcatgcgcac agaagctttg ggattaagaa cgcccaatgg cgaacgttct
     2521 cccatggcca tagcctcacc ttgatcgctt tgaaaatcac gtagggttat tgcacaatcc
     2581 gctaccggca cttgccaagg tcctaccatt tgatctctgt gcactaatcc gccgacactt
     2641 cgatccgcga tggtgattaa aaaagtttta tctgcgacgg ctggaaattg tattatttgt
     2701 tctaacgcct cttttaaatc cattgtgact ttactagcta gttttttcgt tgcatacgta
     2761 tctacaattt ttaaaagctt aggagaacct tctagcatga ggcttattgg aacttgcacg
     2821 ggatacgcgt taaaaagagg atcgtatact tctaaaatgt tagaactcgt cgttgtaccg
     2881 ataacggcat aaggacaacg ttcgcgtttg gctaccttat cgaaaatacc gagcttgtct
     2941 tcttctatgg ccagcacata acgctcttgc gcttcattac accaaatttc taagggtgtc
     3001 atggaaggct ccgcattagg tatttgacgc aagtcaatgt gcgcaccaac gccgccttgc
     3061 gctataagtt caatgacggc attactgagt ccgcct
//
LOCUS       DASTIB010000199         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_377452, whole genome shotgun sequence.
ACCESSION   DASTIB010000199 DASTIB010000000
VERSION     DASTIB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377452"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFP93_03405"
     CDS             complement(<1..1064)
                     /locus_tag="VFP93_03405"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET9843687.1"
                     /translation="MKKTKKDLPGLEAKQLHDQTMLLGKGGDSHQIAEKKQAVLTTQL
                     GTPISDDQNSLKSGDQGPVLMEDFVFREKLFHFDHERIPERVVHARGFGAHGFFENYK
                     PLSDITRAHLFQRKGEKTPVFVRFSTVVGNKGSADLARDVRGFAVKFYTQEGNWDLVG
                     NNIPVFFIQDPIKFPDVVHAAKQEPDRAFPQAATAHDNFWDFVTLTPESMHMLMWTMS
                     DRAIPRSFRFMEGFGVHTFRMINAEGKSTFVKFHWKPKLGLQSVTWDEALKINGADPD
                     FHRRDLWNAIQNEDYPEWELGLQLFDDAFVERFDFDILDSTKLIPEEEVPITIVGRLV
                     LNRCVDNFFAETEQVAFCTQ"
     gene            1274..2449
                     /locus_tag="VFP93_03410"
     CDS             1274..2449
                     /locus_tag="VFP93_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9843688.1"
                     /translation="MHDLTIITMLTTALVAALALGWLTQWVGFSPIVGYLLAGIVVGP
                     HTPGFVANINLANQFAEIGVIFLMFGIGLQFHWRELVKVWKVAIPGALSQSLIATFCG
                     WILARFFGWDSISGIILGMGLAVASTVVMLRMLEEQNYLKKREGQIAVGWLIVEDIFT
                     VIVLVIMPALAVPTDQGANTAHAVFWAIVKVCGFGIFLFLLGPIIISPFLKKMAQSKF
                     KELFTLSVFVFALSIATIASSLFQVSIALGAFLGGLVVAQSGMNSRAAKDILPFRAIF
                     SALFFVSVGMLFDPIFVLDHPVMTLSALTIICIIKPISAFLLVLLLRGKFATAWTIGI
                     CLAQIGEFSFILASLGYSLGLLPDDGYDLFVACALISIALNPLLFRLPFWKSPPSSQ"
     gene            complement(2494..>3548)
                     /locus_tag="VFP93_03415"
     CDS             complement(2494..>3548)
                     /locus_tag="VFP93_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HET9843689.1"
                     /translation="TKIDYTPGQLLGCISGELGLRKYLTELGHKLIVTTEKEGPYFDK
                     ELLDAEIVISQPFWPAYLSAEKIQQAKNLKLIITAGVGSDHIDLNAAVNQGITVAEVT
                     YSNSNSVAEHAVMMILALVRNYIPSYQWIKQGGWNIADCVKTAYDLEGMQVGIIGAGR
                     IGLGTIKRLQGFDVNICYTDRHRLDKNVEQKFGLTFYDNIGALVPHCDVISISCPLHA
                     ETENKFDEALLSKMKKGSYLINTARAKICDSNAIVKALETGQLAGYAGDVWYPQPPNL
                     NHPWRSMPNHGMTPHVSGSTLSAQNRYAAGVREILECWFAKKPIRNEYLIVENQELAG
                     VGKHAYSIHASTKSLA"
BASE COUNT          958 a          705 c          694 g         1191 t
ORIGIN      
        1 ttttgggtac aaaaagcaac ttgttcggtt tcggcaaaaa aattatccac acatcgattt
       61 aatacaagac gtcctactat ggtaatgggc acttcttctt ctggaataag ttttgtcgaa
      121 tcgagtatat caaagtcaaa acgttccaca aatgcatcat cgaatagttg taatcccagc
      181 tcccattctg gataatcttc attttgaatt gcattccaca aatcgcgacg gtgaaaatcc
      241 ggatcggcac cattaatttt cagggcctca tcccacgtta ccgattgtaa accaagcttt
      301 ggtttccaat gaaattttac aaaggttgat ttaccctctg cgttgatcat gcgaaaggta
      361 tgcaccccaa agccttccat aaatcgaaac gaacgtggaa ttgctctatc ggacattgtc
      421 cacattagca tgtgcatact ttcaggggtt aaggtaacaa aatcccaaaa gttatcatgc
      481 gctgtagctg cttgcgggaa tgctcgatcg ggctcttgct ttgcagcatg tactacatca
      541 ggaaatttaa taggatcttg aataaaaaat acagggatat tattgccaac taaatcccaa
      601 ttcccctcct gggtgtagaa ttttacggca aaaccgcgca catctcgtgc taaatccgcg
      661 gatcctttat tcccaactac cgtagaaaaa cgtacgaaga ctggtgtttt ctctcctttg
      721 cgttgaaata aatgcgcacg cgtaatatcg gataagggct tataattttc aaagaatcca
      781 tgggcaccaa atcctcgtgc atgcaccacc cgctcaggaa tgcgctcatg gtcaaaatgg
      841 aacaattttt ctctaaaaac aaaatcttcc attaataccg gaccttgatc cccacttttt
      901 aaagaatttt gatcgtccga tattggggta cctaattggg tagtaagcac tgcttgtttt
      961 ttttccgcta tttgatggct atccccacct ttgcctagca acatagtttg atcgtgtaat
     1021 tgttttgctt ctaacccagg aagatccttt ttggtttttt tcatgttata tccttaatac
     1081 acggtatgat agggtatttt tttaaaattt agctaatcta caaatttgaa aaaatttatg
     1141 gttaacgata gcacctttta tatggaaaag gatctgattt aaacaattta caagtgtttt
     1201 aattttggaa ctatattctt tagtagctta ctgtattttt tgagaagtgc actgcactct
     1261 taaggacgtt gtcttgcatg atttaaccat tattaccatg ttaaccacag ctttggtcgc
     1321 agccttagcg ttaggttggc ttactcaatg ggtgggcttt tcacctattg ttggatattt
     1381 gctagcgggc attgtagtag gacctcatac cccaggattt gtcgccaata taaatcttgc
     1441 aaaccaattt gcagaaattg gggtaatttt tttaatgttt ggcataggtt tacaatttca
     1501 ctggagggaa ctcgtaaagg tgtggaaagt tgccataccg ggtgccttat cgcaaagtct
     1561 tattgccaca ttttgtgggt ggattttagc gcgttttttt ggttgggatt ccatctcagg
     1621 aattatatta ggtatgggtt tagcggtggc gagcactgtt gttatgctgc gcatgttgga
     1681 agagcagaat tatttaaaaa aacgtgaagg tcaaattgca gttggttggc tcattgtcga
     1741 agatattttc acagtgattg tacttgtcat tatgccagca ctagccgtgc caacggatca
     1801 aggggcaaat actgcacatg cggtgttttg ggcaattgtc aaagtatgtg gctttggtat
     1861 ttttcttttt ttgcttgggc caatcattat ttcgcctttc ttaaaaaaaa tggcgcaaag
     1921 caaatttaag gaattattta cgctaagtgt ttttgttttt gcactaagta ttgcgaccat
     1981 tgcgtcatcg ttatttcaag tttctattgc cttgggcgct tttttaggag ggcttgtggt
     2041 agcccaatcg gggatgaact ccagggctgc aaaagatatt ttgccgtttc gagcgatttt
     2101 ttcggcatta ttttttgtgt ccgtaggcat gttgtttgat cccatctttg tcttggatca
     2161 tcccgttatg acgctttctg ctttaacaat tatatgcatc attaaaccaa ttagcgcatt
     2221 ccttttggtg ttattattac gcggaaaatt tgctactgcc tggaccattg gcatttgttt
     2281 ggcacaaata ggagaatttt cgtttatttt ggctagctta ggctatagtt taggacttct
     2341 tcctgatgac ggttatgatc tatttgtagc gtgcgcttta atttctatcg cattaaatcc
     2401 tttactattc cgattacctt tttggaaatc acctccttca tcacaataaa aattaacaaa
     2461 gaaagtgtcc ctcagacact ttcttttacc ttattacgct aaggacttgg tcgatgcatg
     2521 gattgaataa gcatgcttac ctacgcctgc caattcttgg ttttctacaa tgagatattc
     2581 atttcgaata ggtttctttg caaaccagca ctctaaaatt tctcttaccc ctgctgcata
     2641 tcgattttgt gcagaaagcg ttgaacctga aacgtggggg gtcataccgt gattgggcat
     2701 cgatcgccat ggatgattta aattgggtgg ttgcggatac cacacatcgc ctgcatatcc
     2761 tgcaagttgg ccagtttcta aagcttttac aattgcgtta ctatcacaaa tttttgcgcg
     2821 tgcagtatta attaaatatg atcctttttt cattttgcta agtaatgctt catcgaattt
     2881 attttcggtt tctgcatgca aaggacaact aatagaaata acgtcacaat ggggaacaag
     2941 tgctccaatg ttatcataga aggttaaacc aaatttttgc tccacatttt tatcaagacg
     3001 atggcgatcg gtgtaacaaa tgttcacatc aaatccttgc aaacgtttaa tggtaccaag
     3061 cccaatgcga cctgcaccta tgattcctac ttgcattcct tctaagtcgt acgctgtttt
     3121 cacacaatcg gcaatgttcc atcctccttg ctttatccat tgatacgatg gaatataatt
     3181 gcgcactaat gccaaaatca tcataacggc atgctcggca acactattgc tattggaata
     3241 ggtaacttca gcaaccgtta tcccttggtt aacagcggca tttaaatcga tgtgatcgga
     3301 tcctactcca gcggtaataa tgagttttaa attttttgct tgttgaattt tttctgccga
     3361 taaataagcc ggccaaaaag gttgggagat cacaatttca gcatcaagca gttctttatc
     3421 aaagtatggt ccttcttttt cggtagtaac aataagcttg tgacctaatt cggttaaata
     3481 ttttcgcaaa cctaactcac cagaaataca tcctaaaagt tgaccaggtg tatagtcaat
     3541 ttttgttg
//
LOCUS       DASTIB010000200         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_35573, whole genome shotgun sequence.
ACCESSION   DASTIB010000200 DASTIB010000000
VERSION     DASTIB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35573"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..175
                     /locus_tag="VFP93_03420"
     CDS             2..175
                     /locus_tag="VFP93_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HET9843690.1"
                     /translation="MYIGREDFEATEWHEAQREAKRNLSPNLTDYLFAKPYLADYLEK
                     GLSLLGYSCEDTE"
     gene            complement(178..369)
                     /locus_tag="VFP93_03425"
                     /pseudo
     CDS             complement(178..369)
                     /locus_tag="VFP93_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
     gene            673..1035
                     /locus_tag="VFP93_03430"
     CDS             673..1035
                     /locus_tag="VFP93_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843691.1"
                     /translation="MKYLVVLLSLFPLQVAYSNCPQVCKGTEPFWNAEISEKSLTFQK
                     IDEESQTYTLENVQHALGLQDDFLTKYQLRQEGAVKGTIFLLKTGNCSDGMSDEIFPY
                     SVILDRDNDASLHYGCCK"
     gene            1198..1551
                     /locus_tag="VFP93_03435"
     CDS             1198..1551
                     /locus_tag="VFP93_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843692.1"
                     /translation="MYSYDTIVSIYYSRELTDVQKLVSILKMPMDVFRHQPSCRILDT
                     LFVENEEIRKEIIYYYFEVFPNLNVQQVIHDYQKLVTNDSIYAQLRLSHNHVLLDIFL
                     ARMDVPLEILDPSIM"
     gene            1645..1908
                     /locus_tag="VFP93_03440"
     CDS             1645..1908
                     /locus_tag="VFP93_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HET9843693.1"
                     /translation="MKGESHAQTHTTHARVQPHDKVDDFVDATADRAKRAAHATTEQY
                     ENIKEEITDQVDNVGEYISKNPFKSALIAAGVGMLFGIMLKKK"
BASE COUNT          777 a          444 c          490 g          908 t
ORIGIN      
        1 tttgtatatt ggcagagaag attttgaagc aaccgaatgg catgaagcac aacgagaagc
       61 caagagaaat ttaagcccca atttgaccga ctatttattt gcaaaacctt atctcgccga
      121 ttaccttgaa aaaggtctta gtttattggg ttattcttgt gaggatacag aatagtatta
      181 agaagatttt ttgttcgtct ttttgggaac tttagcccct ttctttctcg cttttgatag
      241 tcctattgca atcgcttgtt ttctactggt aacttttctc tttttaggac cactatgtgc
      301 ccgtcctctt ttaaattcgt gcatttcttt ttctacagag tcttgtgctt ttttatcgta
      361 tttgcgcgag gatttcttcg tagaagtcga ctttttggat gcagaagatt ttttggtagc
      421 gcttgtagat gtttttttcg acgtgctgct tggctttttt ttgctggagg tggctttttt
      481 ggttgtagac tttttttgta cactactggg tttattgttt gtttttttcg ttgatttttg
      541 ggatgctgtt tttgccattt actacactcc ataaattata ttttttaagt atagaaggcc
      601 acaaaaaaat tttatgcgca aggatttttt cccagtttat tatcaactaa tcttatagtt
      661 aaggagaagc gtatgaaata cctagttgta ctgttaagtt tatttcctct gcaagtggca
      721 tatagtaatt gtcctcaagt atgcaaagga accgagccat tttggaatgc tgagatttca
      781 gaaaaaagct taacttttca aaaaattgat gaagagtcac aaacatatac tttagagaac
      841 gtgcagcatg ctttagggtt acaagacgat tttttaacca aataccaact tcggcaggaa
      901 ggcgccgtta aagggaccat ttttctacta aaaaccggaa attgttccga tggtatgtcg
      961 gatgaaatat ttccttacag cgttattctc gatagagata atgatgcttc tttacattat
     1021 ggctgttgta aataatggta agcagtattt tatagttaaa caactttcta aagaaatgct
     1081 gcaaaatctt caacaaatca ctttaatcgc acaacggtta acgtttatct ttccttgata
     1141 ttttcttacc aaaaaaaaac ttaaaatttt tttgtgaaat ttttgcggag agagattatg
     1201 tattcctacg ataccattgt gagcatttat tacagccgag aattaaccga tgtccaaaaa
     1261 ctagtcagca ttttaaaaat gcctatggac gtctttcgac atcaacctag ttgtcgaatt
     1321 ttggacacct tattcgtgga aaatgaagaa atacgcaaag aaataattta ttattatttc
     1381 gaagtatttc ccaatttaaa tgtacagcaa gtcatccacg attatcaaaa actggttacc
     1441 aacgattcta tttatgcaca gcttcggcta agtcacaatc acgtgttatt agatatattc
     1501 ttagcgagaa tggatgttcc cttagagatt ttagatccaa gcattatgta atcgcagtga
     1561 aattaaagta agtaagtggt tctttggtgt ttgagaaacc tgtgctattt ttatagatgt
     1621 acaaattcaa atattggggg aatcatgaaa ggagaatcac acgcacaaac tcatacaaca
     1681 catgcaaggg tacaaccgca tgataaagta gatgattttg tagacgcaac cgcagatcgc
     1741 gctaaacgtg ctgcccacgc aactacggag caatacgaaa atattaaaga agaaattacc
     1801 gatcaagtag ataatgtcgg ggaatatata tcaaaaaatc cttttaaatc tgcgctgatt
     1861 gcagccggag taggaatgct ttttggtatt atgttgaaaa agaaatgata aataacccaa
     1921 aaaatgattc ggagcctcat gtacttgagg cttttcatgc ttatctgaat gcgatcgcac
     1981 tgcaagctaa aattggttgg cttgaactaa aattggcggt atcgaccgtc ccttggttat
     2041 ttgcgagtat ggttgttttc gctttctgtg gtttgtcttt ttggttgcta ttggttactt
     2101 tcattgtcgt gctttttatg catcttggaa tgtcaatttt gatgagtctt ttcctagcat
     2161 tgttgttaca cagctctttg ttgctatttg ctttaatttc actaaaaaaa ttatttctga
     2221 atttgcagtt tgtagaatcc cgaaaaataa tgaaaggtta caaccaccat gtttaacagg
     2281 aaaatggatg tgttacacgc cgaaattgcc atagcgcgcc agcgctatga ttattacatt
     2341 tacaaaggga ctaggatcta ccaacatgaa agttatttaa ccaaacattg gcatattgca
     2401 ctgttttttt ctgttttgtt ttgtttaggc tatattagtg ctggttcttc gagactcagc
     2461 cgattggtac aattcattac catgaaatat ataccctatg tgaataaaat tgtttaaagc
     2521 ataggtttat cgttgtgttt aagtccgtaa caagccccta aacaagatcc tattacacca
     2581 gataacaata taaagaaact ggctataccc gtccatccc
//
LOCUS       DASTIB010000201         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_822960, whole genome shotgun sequence.
ACCESSION   DASTIB010000201 DASTIB010000000
VERSION     DASTIB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822960"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1045
                     /locus_tag="VFP93_03445"
     CDS             116..1045
                     /locus_tag="VFP93_03445"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET9843694.1"
                     /translation="MGGNSAERNISLETGQAILDALIRKKINACAIDATNNFVNELIQ
                     GQFDRVFIALHGTQGEDGCVQGVLECLGIPYTGSGVAASALAMDKARTKLVLDGLGLP
                     TPPFGIAYHLSQAERIAQSIGCPLAVKPISEGSTIGGSKVTHLDELPKAFKNARDYGP
                     VLIERWINGIDMFVSILYDQALPSVQVKPASGIYDFKAKYIANDTLYLCPAPLSNQQE
                     EEIRALSKKAFYALGCEGWGRVDLVLDDQGKFWILEVNTIPGMTSHSLVPLSAKVAGI
                     NFDDLVLGILATSMKSKTIISETQHTNPATKAK"
     gene            1048..1806
                     /locus_tag="VFP93_03450"
     CDS             1048..1806
                     /locus_tag="VFP93_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HET9843695.1"
                     /translation="MEVPRKSRFKRSSISRYIPLKKFLVGFSLGLFVVLLASSIYLLS
                     SLKLFPIRYVEVVGNHEHVPHVEIYEVMQPGLLKGFFGLSVNRLRKELCSLPWIKHAI
                     VRRVWPNKVLIKIVEHEPLAVWNNQAILTKNGALITPKNVSLVESVPYFFGPEGKYHQ
                     IIAEWRKMGETLAKIDLNITHLELAPRGAWQLTLDNGITVRLGTHDIQSRLDRFVRAY
                     DKVLFKQEKTIEYVDLRYTIGLAIGWKPSSIHNS"
     gene            1866..>2064
                     /locus_tag="VFP93_03455"
     CDS             1866..>2064
                     /locus_tag="VFP93_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9843696.1"
                     /translation="MSKKSEKNLIVGLDIGTSKVVTIVGEITDDGLDVIGIGSHPSYG
                     LKKGVVVNIESTVQSIQRAVEE"
BASE COUNT          622 a          347 c          447 g          648 t
ORIGIN      
        1 cattcctgtg tcaaccctaa tccttaaaaa ttctgaacat ttttttgctt atacgcgatc
       61 tttttttaac gcaacttaca aggaccctaa agcgtttggg aaagtagcgg tattaatggg
      121 tggcaattct gcagagcgaa acatttcttt agaaacgggt caagccattt tagacgcact
      181 aatacgtaaa aagattaatg cttgtgcgat cgatgctacg aataattttg ttaacgaact
      241 catccaggga caatttgatc gcgtttttat tgcacttcac ggtacacaag gggaagatgg
      301 ctgtgtgcaa ggggttttag aatgtttagg aattccttac accggtagcg gtgttgcagc
      361 gtctgcttta gcgatggata aggctcgaac taaactggta ttagatggtt tggggctgcc
      421 aacgccacct ttcggcatag cttatcattt atcgcaagcc gaacgtatcg cgcaatccat
      481 aggttgtccg cttgctgtta aacctatttc agaaggttca accataggcg gcagtaaagt
      541 tacacattta gatgaactgc caaaagcttt taaaaacgcg cgtgattatg gtccggtttt
      601 aattgaacgc tggattaatg gaattgatat gtttgtgtcc attttatacg atcaagcact
      661 tccttctgtg caggtcaaac cagcatcagg aatttatgac tttaaagcaa aatatattgc
      721 aaatgatacc ttgtatttat gtccagctcc cctttcaaac caacaagaag aggaaataag
      781 agcgctttcg aaaaaagctt tttatgcatt aggttgcgaa ggatggggcc gtgtggattt
      841 ggtgttagat gatcagggta aattctggat tttggaagtt aatactatac ctggaatgac
      901 ctctcatagt ttagttcctt tgtctgccaa agttgctggg ataaattttg atgatttagt
      961 tttaggcatt ttagcgactt ctatgaaatc aaagactata atcagcgaaa cccaacatac
     1021 aaatcctgca actaaagcta agtagttatg gaagtaccta gaaagagtcg gtttaagcga
     1081 tcgagtattt cgaggtatat ccctttaaaa aaatttcttg taggctttag cttgggtttg
     1141 tttgttgttt tgttagcgag ttcgatttac ttactttcca gtttaaagct gtttcccatt
     1201 cgttatgtgg aagttgttgg aaatcacgag catgttcctc atgtagaaat atatgaagtg
     1261 atgcagcctg gattgttaaa aggttttttt ggattaagcg taaatcgatt acgaaaggaa
     1321 ttatgttctt taccttggat taagcatgca atcgtcaggc gggtatggcc gaataaagtc
     1381 cttattaaaa ttgttgaaca tgagccactt gcagtttgga ataaccaagc gattcttact
     1441 aagaatggtg ccttaataac accaaaaaat gttagtttgg tggagtctgt tccgtatttt
     1501 ttcggacctg aaggaaaata tcatcaaatt attgctgaat ggcgaaaaat gggggagacg
     1561 ttagccaaaa ttgatcttaa tattacacat ttagaattag ctcctagagg agcttggcaa
     1621 cttacattgg ataatggcat tacggtgcgt ttaggcactc atgatataca atcacgttta
     1681 gatcgatttg tacgtgccta cgataaagtt ttgtttaaac aagaaaaaac gattgagtat
     1741 gttgatctac gctatacaat tggtttagcg atcggttgga agccatcttc catccataac
     1801 tcctaagtaa aaaatagata gaaaatatag cttttgagct gacggaccaa ggagaccgtt
     1861 tgttcatgtc aaaaaaatct gaaaaaaatt taattgtagg cttagacatc ggcacttcta
     1921 aggttgttac tattgttggc gaaataactg atgatggctt ggacgttata ggaattggat
     1981 ctcatccatc ttacggttta aaaaaaggcg tagtagtcaa cattgaatcg acagttcaat
     2041 ctattcaacg agcagtagaa gaag
//
LOCUS       DASTIB010000202         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_204190, whole genome shotgun sequence.
ACCESSION   DASTIB010000202 DASTIB010000000
VERSION     DASTIB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204190"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1889)
                     /locus_tag="VFP93_03460"
     CDS             complement(21..1889)
                     /locus_tag="VFP93_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9843697.1"
                     /translation="MLLSYLPNLYAHPFFQRADLESNTTSIFASAAEMCAAQKGEHMK
                     KVVGSLLVTLVLGYTIQLIAAAKPSSLAQTLEVVKTVDKDLLSKSELSSENLLKILSA
                     EMALNREEPEVALKNYIAVAKETQDPAIAQRATQIALSIASLEDALLPAEIWAKNDPK
                     NLEAQITVAAIFIRIAKPQKSVPYLKKLMEIDPIDSDRHFVMLYKQLTSSADQQNVLK
                     ALELVSKDAPKSNLAHVALADIAIYDNRLQDALRLAQDALKIQHKDPKATILYAQALL
                     QNSELDKANAFLEKQLKAIPDSLPLMQYYTQFLIEQKALDKAQIQMNLLTENKALSTR
                     QLLHLSQVSMQVGWYDQAEKLLHKVRDDKEHVHTANYFLARIYEERNNIPEAVTWYEK
                     VQEGPFHVISYIRASVLLADSKHYEKALGLLNYVEPQNYADVKRIALAKVDIFSRSKE
                     NQKALDLLNALLQQEPEDLDYLYSRALIYEKLSQIKNTEADFKKILEIDPNHVDALNA
                     LGFILADHTERFSEAKEYIEKALKLAPDNPSVLDSLGWVHYKMGNTKLAIETLRKALS
                     LFPDPEIAAHLGEVLWSSNQQAEATKVWNEALEASPNDQRVIKTMERLVGETHKSN"
     gene            1888..>2613
                     /gene="hemA"
                     /locus_tag="VFP93_03465"
     CDS             1888..>2613
                     /gene="hemA"
                     /locus_tag="VFP93_03465"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9843698.1"
                     /translation="MIVAIGISHKTATVAVREKVAFTADLVPLILRQAKNKSKAQELV
                     LLSTCNRTEFYGWIEHPNNIVAWWEQQNVQLPNLSNYLYYYTDISAVRHIMRVASGLD
                     SMVVGEPQILSQLKQAYDIACDQKSIGNYLQRLFQKSFMVAKQVRTETAIGRNPVSVA
                     YSAVKLSQKIFTDLKKARVLLIGAGETIELVAQHLKAQGVTQQTYVNRSFDRATVLAN
                     HMQGNVIPFEQMTDHISDFDIIIS"
BASE COUNT          740 a          524 c          518 g          831 t
ORIGIN      
        1 aaaaaatcaa agtatatcgt ctaattggat ttgtgggttt cacccacaag tctttccatc
       61 gttttaatta ctctttgatc atttgggctg gcttctaaag cttcattcca cactttggtg
      121 gcttccgctt gttgatttga gctccaaagc acttccccta aatgcgctgc tatttctgga
      181 tccggaaata aactcagtgc ttttcgcaag gtttctatgg cgagctttgt gttgcccatt
      241 ttatagtgca cccatcccaa agaatctaaa acggacgggt tatcgggggc aagcttcaaa
      301 gctttttcaa tatattcttt tgcttcgctg aaacgctcag tatgatccgc taaaataaat
      361 cccagtgcat tcaaggcatc cacatggttc ggatcaattt ctaatatttt cttaaagtcc
      421 gcttccgtat tttttatttg cgaaagcttt tcataaataa gagctcggga gtacaaataa
      481 tctagatcct ccggctcttg ttgcaataag gcatttaaca agtcgagtgc tttttgattt
      541 tctttgcttc tcgaaaaaat atccacttta gccaacgcaa tgcgttttac atccgcataa
      601 ttttgaggtt ctacataatt taacaatcca agagcttttt catagtgttt cgaatctgcg
      661 agcaaaacgc tggcgcgaat ataagaaatg acatgaaagg gaccttcttg aactttttca
      721 taccacgtta ccgcttcggg aatattattg cgctcctcat agatgcgtgc caagaaataa
      781 ttggcggtat ggacatgctc tttatcgtca cgtactttat gtagcaattt ttctgcctgg
      841 tcataccacc ctacctgcat gcttacttgc gaaagatgta ataattgtct tgtactgagc
      901 gctttatttt cggttagaag attcatttgt atttgcgctt tatctaacgc tttttgttca
      961 ataagaaatt gcgtatagta ctgcattaaa ggtaggctat cgggaattgc ttttaattgt
     1021 ttttctaaga aagcattcgc tttatcgagc tcgctatttt gcaataaagc ttgcgcatat
     1081 aaaatagtag ccttaggatc cttgtgttgt atttttaacg catcttgcgc caaacgtaac
     1141 gcatcttgta gccgattgtc ataaattgca atatccgcta aggctacatg cgctaaatta
     1201 cttttaggag catctttcga aactaattct aatgccttga gaacattctg ttgatcggca
     1261 gaagaggtaa gttgtttgta aagcattaca aagtgtcgat ccgaatcgat aggatctatt
     1321 tccattaatt tttttaaata aggaaccgac ttttggggct tagcaatacg aataaaaatg
     1381 gctgccacgg ttatttgggc ttctaaattt tttggatcat tttttgccca aatctcagct
     1441 ggtaataatg catcctcaag acttgcgata cttaaagcaa tttgggttgc acgttgcgct
     1501 atagcgggat cttgggtttc tttcgctaca gcgatataat ttttcagtgc gacttcaggc
     1561 tcttcacgat ttaaagccat ttcagcactt aatattttaa gtaaattttc tgatgatagt
     1621 tccgacttag ataaaagatc tttatcgacc gtcttcacga cttctaaggt ttgagctaat
     1681 gatgagggtt ttgcagctgc gataagttgg atagtgtacc ctaagacaag ggttaccaat
     1741 agtgatccca ctaccttctt catatgttct cccttttgtg cagcgcacat ttcggctgca
     1801 gatgcaaata tggatgtcgt gttactttct aaatcggcac gttggaaaaa agggtgagca
     1861 tataaattcg gcaaataact gagtagcatg atagtagcaa tcggaattag tcataaaaca
     1921 gcgaccgttg cggtgcgaga aaaggttgca tttacggcag acctcgtacc cttaatactg
     1981 aggcaggcaa aaaataaaag caaagcgcaa gaattggttt tactttctac ttgtaaccga
     2041 acagaatttt atggatggat agaacatccg aacaacatcg tagcgtggtg ggaacagcaa
     2101 aacgttcagc tccctaattt atccaattat ttatattatt acaccgacat cagcgcagtt
     2161 cgacatatca tgcgggtggc atctggcctc gattctatgg ttgtaggtga gccccaaatc
     2221 ttaagccaac taaaacaagc ttatgatatt gcttgtgatc aaaaaagcat tggcaactac
     2281 ttgcaacgtt tattccaaaa aagtttcatg gttgcgaagc aagtaagaac cgaaaccgcc
     2341 attggacgca atccagtttc tgtcgcgtat tctgcggtaa agttgtccca aaagattttt
     2401 accgatttaa aaaaagcacg cgttcttctc attggcgccg gggaaaccat tgaattagtg
     2461 gcgcaacatt taaaagccca gggtgtaacg caacaaacgt acgtaaaccg ttcgttcgat
     2521 cgagcaacag ttttggcaaa tcacatgcaa ggcaatgtga ttccttttga acagatgact
     2581 gaccacatca gtgatttcga tataattatt agc
//
LOCUS       DASTIB010000203         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_715842, whole genome shotgun sequence.
ACCESSION   DASTIB010000203 DASTIB010000000
VERSION     DASTIB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715842"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFP93_03470"
     CDS             complement(<1..992)
                     /locus_tag="VFP93_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843699.1"
                     /translation="MLNNAFLHAEDINFSLLTAIKTGNENRVREILDSLQRPILENIR
                     DEEDYKPNLLIMGSHSPAIVVELLKAYPNISSCEKNLALIHAASRGCTQTIVEILTRC
                     SDIKRSDQAASIICAAKCGQIQAFVELILRCWADLTHNDKEMVLFEISDYGEPLVELI
                     NRCGTEINSDLKGFTLRRAARNGNYQTIVELLARWNDEISNIDKGEALDNAASRGHSQ
                     VIREILNRCDAEIESEDKAFALIHAVECVHLNALNTLLAYPSVEEVANYDNNQALTEA
                     LTRADSNSGYRLQYQLIVDRLMQIPAVANLTAQQSQQAMIDLSYITQFAENSMQA"
     gene            complement(1249..2385)
                     /gene="dapE"
                     /locus_tag="VFP93_03475"
     CDS             complement(1249..2385)
                     /gene="dapE"
                     /locus_tag="VFP93_03475"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET9843700.1"
                     /translation="MILDAINLAIELIKKPSVSPEDAGCIDLIVKYLQPYGFQCEIFH
                     CEGVTNLWATHGTQSPCLSLIGHTDVVPPGEETLWSTPPFEPVIEDNILYGRGACDMK
                     GALAAMVIAACEYVQQYPNHAGTLAFIITSDEEGKALFGTRKVVEWLQNSKKTIEYAL
                     VGEPSSVNQLADTIKIGRRGSLTGNLTIQGIQGHVAYPQLAKNPIHQAFEVLHTLTQK
                     QWGDEHPDFPLTSLQFSNIQAGTGADNVIPDSLTTIFNFRYNPTITHTELIASFEQEL
                     QNNAINYTVEWHHSGSPFITSRNSKLLQTLLETLKEQKYPDPTLSTSGGTSDGRFLGA
                     IGTEVVEIGPVNKTIHQINECVDIEQLKALQKIYFQVFRRILIQ"
     gene            complement(2386..>2727)
                     /locus_tag="VFP93_03480"
     CDS             complement(2386..>2727)
                     /locus_tag="VFP93_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET9843701.1"
                     /translation="VGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMG
                     VYIGQSTKIYDRENDKIYYGRVPKNSVVVAGSLPSKEGKYALNCAIIVKRVDAKTKSK
                     VGINEILREIC"
BASE COUNT          826 a          542 c          509 g          850 t
ORIGIN      
        1 gcttgcatgg agttttcagc aaattgcgtg atgtaagata agtcaatcat ggcttgctgt
       61 gattgttgag cagttaagtt tgctacggct ggtatttgca ttaacctatc tacgataagt
      121 tgatattgta atctatagcc agaattgcta tccgcccgcg ttaaggcttc cgtgagtgcc
      181 tgattattgt cataattcgc aacttcttcc acgcttggat atgctagcaa agtatttaat
      241 gcatttaagt gaacacattc aacagcgtgt atcagagcaa aagctttatc ttcgctctct
      301 atttcagcgt cgcagcgatt cagtatttct ctgataactt gagaatgccc gcgtgatgcg
      361 gcgttatcta gtgcctcacc tttatctata ttgcttatct catcattcca gcgagcaaga
      421 agttcaacaa tcgtttgata attcccattt cgtgcagcac gtcgaagagt aaaacccttc
      481 aaatcactgt ttatttcagt tccacaacga ttgatgagtt cgacaagagg ttccccatag
      541 tcagaaattt caaacaacac catttcctta tcattatgag ttaaatcagc ccagcagcga
      601 aggatgagct caacaaaagc ttgaatctga ccacatttag cggcacatat aatggaagct
      661 gcttgatcac tacgttttat atcactgcaa cgggtgagaa tttcaacaat agtttgagta
      721 catcctcggg aagcggcatg tattagcgcg agatttttct cgcaagaact aatattaggg
      781 tatgctttaa gcagctctac tacaatagct ggtgaatgac ttcccattat taataaatta
      841 ggcttatagt cttcttcatc tctaatattc tctaagatag ggcgttgtaa agaatctaaa
      901 atttccctta ccctattttc atttccagtt ttaattgccg ttaaaagtga aaaatttata
      961 tcttcagcat gtaaaaaagc attatttaac atacatcact catataataa tttagtagat
     1021 catatcaaaa aaaaagaaaa gcatgcaatg aatcaaggcg aaaaaatcgg catattattt
     1081 tgaatattgc tgataagcca gcaagaaaaa cgggtgtcac aaaaaattta atctctgcaa
     1141 aaatctatat agtaaattta agatcgcaat tatttagaac attgtttatg ctaaaaagaa
     1201 tgcttcaaaa catgaattta ttcttaagtt aaaaaagcat gaaaattcct attgtataag
     1261 aattctccga aatacttgaa aataaatttt ctgcaacgct tttaattgtt caatgtcgac
     1321 acattcatta atttgatgaa ttgttttgtt cacaggaccg atttctacaa cttcggtacc
     1381 gatggcgcct aaaaatcgcc catcggaagt tcctcccgag gtagataatg ttggatctgg
     1441 atatttttgt tctttgagtg tttcaagtaa tgtttgtaaa agttttgaat tgcgtgaagt
     1501 aataaaagga gaacctgaat gatgccattc tacagtatag tttatggcgt tattttgtaa
     1561 ttcttgttca aagctcgcta ttaattctgt atgggtgatt gtagggttgt agcgaaaatt
     1621 aaaaatagtg gttaaagaat cggggatcac attatccgcg ccagtccctg cttgaatatt
     1681 agaaaactgc aacgaagtta gtggaaaatc tggatgttca tcaccccatt gtttttgggt
     1741 gagtgtatgc agcacttcaa aggcttgatg aatagggttt ttagctaatt gcggatacgc
     1801 tacatggcct tgaattcctt ggatggtaag gttgccggtt agggatccac gccggccaat
     1861 ctttatcgta tctgcaagct ggttcacact agaaggctca ccgactaatg catattctat
     1921 cgttttctta gaattttgta accactcaac cactttgcgt gtcccaaaaa gtgctttacc
     1981 ttcttcatcg ctggtgataa taaaagctaa agtacccgca tgattgggat attgttgcac
     2041 atattcgcag gcagcaatca ccatagccgc gagcgcacct ttcatatcac acgcaccacg
     2101 accatacaag atattatctt caataacagg ttcaaaggga ggagtggacc acagggtttc
     2161 ttctcccgga ggaaccacgt cggtatgacc aattaaactt aaacacgggc tttgcgtacc
     2221 gtgtgtagcc caaagattag taacgccttc gcaatgaaaa atttcgcatt gaaagccata
     2281 gggttgcaaa tatttgacga ttaagtcgat acatcccgca tcctctggtg aaacggaagg
     2341 ctttttgata agctcaattg ctaaatttat tgcatctaaa atcatttaac aaatttctcg
     2401 taatatttcg ttgataccta ctttgctttt agttttagca tcaacacgct tcacaatgat
     2461 ggcgcaattt aacgcatact taccttcttt tgagggcagg ctaccggcaa ccacaacaga
     2521 attctttgga acgcgaccat agtaaatttt atcgttttca cgatcgtata ttttggtgct
     2581 ttgccctata tacacgccca tcgaaatgac ggatccttct tcaacaatca ctccctcaac
     2641 aatttcggat ctagcaccga taaaacaatt atcttcaata atagtagggg atgcttgcag
     2701 tggttctagc acaccgccaa taccgac
//
LOCUS       DASTIB010000204         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1143546, whole genome shotgun sequence.
ACCESSION   DASTIB010000204 DASTIB010000000
VERSION     DASTIB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143546"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFP93_03485"
     CDS             <1..471
                     /locus_tag="VFP93_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013661420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9843702.1"
                     /translation="QIAKDYPEVHLTHMYVDNAAMQLIRKPKQFDVIVTSNLFGDILS
                     DSAAMLTGSIGMLPSASLNASNFGLYEPVHGSAPNIANLNQANPLGLLLSLSMMLRYS
                     LDALELANLLELAIQDVLQQGWRTMDIHCEDTTLLSCSQMGDKVAASLQKLLLQ"
     gene            482..1495
                     /locus_tag="VFP93_03490"
     CDS             482..1495
                     /locus_tag="VFP93_03490"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET9843703.1"
                     /translation="MQKKINVAVVGATGLIGETMIRILEEKNFPVDRLYLLASERSKG
                     KSLGFRNEHLKVEDLSSFDFHKVELALFSAGGAVSRQFVPIATKSGCLVIDNTSEFRY
                     NDDVPLVVPEVNAHQIQFWEKKHIIANPNCSTIQMCVALYPLHLQAKIKRINVATYQS
                     VSGAGRSALETLKAEVSGKIYPSSPFPLPIANNAIAHIDRFLENGYTKEEMKMVWETR
                     KIFNEPSLLVNPTAVRIPVSIGHSEVVHIETAIKLGVEEARTILKKSPGITVKDERMS
                     GGYPSPLLDASGKDNVFVGRIREDISHPNGLNMWIVADNVRKGGALNSIQIAQHWFEF
                     CYL"
     gene            1622..>2481
                     /locus_tag="VFP93_03495"
     CDS             1622..>2481
                     /locus_tag="VFP93_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HET9843704.1"
                     /translation="MKKLKKLGLYGLLWSSLFTYPVYCFGVGLGNIQVNTHLNQPLQA
                     IIPLHTLNDVSPTEIKVRLADTNAFEKAGLERPFYLTEIDFKVVERDNQTFIEISSVE
                     PIQDPFLSFVLEANWASGRILREYTVLLDPPSKESVNLAHKLQPKIVPIPRATEKPKK
                     ETGEIFGPVGPEHTLWNIATVVSSKTGGDIHQSVIALAKKNPQAFIGGNINKLRKGTT
                     LQLPNKEEVFQLSISEAIVQVKELITETKSLAKQPQEPIKPLESSMESQQRQPVVEKP
                     LKLMVPGSTTS"
BASE COUNT          810 a          448 c          507 g          716 t
ORIGIN      
        1 caaatagcca aagattatcc agaagtgcat ttaacgcata tgtatgtcga taatgcagct
       61 atgcaactta ttagaaaacc aaagcaattt gatgttatag taacaagcaa tttatttggg
      121 gatattttat ccgacagtgc agctatgcta acaggatcca ttggaatgct gccttcagct
      181 tccctcaatg cttcaaattt tggtttatat gagcctgtcc atggttcagc gccgaatatt
      241 gcaaatttga atcaagcgaa tccccttggg ctcttattgt ctttatctat gatgttgcgc
      301 tattcgttag acgctttaga attagccaat ttacttgaat tagctattca agacgtttta
      361 caacaaggat ggcgaacgat ggatatacat tgtgaagata caacccttct atcctgctcg
      421 caaatgggtg ataaagttgc tgcatctttg caaaaattat tacttcaatg atggataaac
      481 tatgcaaaaa aagatcaatg tagcggtggt aggcgcaaca ggtttaatcg gcgaaacaat
      541 gattcgtata ttggaagaaa aaaattttcc tgtggatcgg ttgtaccttt tagccagtga
      601 aagatcgaaa ggaaaaagtt tggggtttcg taacgagcat ctcaaagttg aagatctttc
      661 aagttttgat tttcataagg tggagctagc tttattctcg gcagggggcg cagtatcgcg
      721 ccagtttgtg cccatagcta ccaagagtgg ttgtttggtt attgataata cttccgagtt
      781 tcggtacaac gacgatgttc ccctggtcgt gcctgaagtt aatgcacacc agatacaatt
      841 ttgggaaaaa aagcatatta tagcaaatcc caattgttca actattcaga tgtgtgtggc
      901 tttatatcct ttacatctac aagcaaagat taaacgcatt aatgtggcga catatcagtc
      961 agtatcagga gcaggtcgta gtgctctaga aacactcaaa gctgaagttt ctggtaagat
     1021 ttatccttca agtccctttc ccttgcctat cgcgaacaat gcaattgcgc atatcgatcg
     1081 gtttttagag aatgggtata ccaaagaaga aatgaaaatg gtgtgggaga ctagaaaaat
     1141 attcaatgag ccttctttgt tggtaaaccc aactgcagtg cgaattcctg tatctattgg
     1201 acacagtgaa gttgttcaca ttgaaacagc aataaaactt ggcgtcgaag aagcgcgcac
     1261 catactgaaa aagagtccag gtatcacggt aaaagatgaa cgcatgtcag gaggttaccc
     1321 tagtcctttg ttggatgcta gtggcaaaga taatgttttt gtaggtcgta ttcgtgaaga
     1381 tatttcgcat ccaaacggat taaatatgtg gatagttgca gataatgttc gaaaaggggg
     1441 ggcattaaac agcatacaga tcgcacaaca ttggttcgag ttttgttacc tttaatagaa
     1501 gatcattctt cgtagataaa ccaaagaagt tcatctatta tcatatatgg ttatccaatc
     1561 tagatattta gtcgctgtta tttattggtg taaactaaca aatattaagt ttggggatac
     1621 ggtgaagaaa ttaaaaaaac ttggtttata cggattactc tggtcatccc tgtttactta
     1681 tccagtttat tgttttggag ttggattagg caatattcaa gtaaacaccc atttaaatca
     1741 acctttgcaa gcgattattc cattacacac actcaatgac gtttctccaa cagaaattaa
     1801 agtacggtta gcagacacta atgcctttga aaaagcagga ctagagcgac ccttttattt
     1861 aactgaaatt gattttaaag tagtcgaaag agacaaccaa acctttattg agatttcgag
     1921 tgttgaacca attcaagatc cttttttatc gtttgtgtta gaagcgaatt gggcatcggg
     1981 tagaatatta cgcgaatata cggttttatt agatccacct tccaaagaga gtgtgaacct
     2041 ggcgcataaa ttgcaaccaa aaatagtacc tattccacga gctaccgaaa aaccgaaaaa
     2101 agaaacgggt gaaattttcg ggccggtagg acctgaacat actttatgga atattgcaac
     2161 ggtagtttct tcaaaaacgg gtggagatat acatcaaagc gtcatagctt tggcgaaaaa
     2221 aaatccgcaa gcctttattg gcggtaatat caataaatta agaaagggta ccactttaca
     2281 attacccaac aaagaagaag tattccaact aagtatctca gaagcgatag tccaagtaaa
     2341 agagttaatt acggaaacga aatcactggc aaaacaaccc caagaaccaa taaaaccact
     2401 tgaaagttca atggaaagcc aacagcggca acccgtagtc gaaaagcctt taaaattaat
     2461 ggttcctggt tcaacaacct c
//
LOCUS       DASTIB010000205         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1390246, whole genome shotgun sequence.
ACCESSION   DASTIB010000205 DASTIB010000000
VERSION     DASTIB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390246"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VFP93_03500"
     CDS             <1..978
                     /locus_tag="VFP93_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843705.1"
                     /translation="QLLSLDLLNLNDELVELCWNISLENADIQILKAFHDYYVKSTTP
                     AFQYFLKNGLTKKNYDKFLLLDRDNAGENIPEITIEGAEIKHPGFYLKKVCAKNDQEA
                     ARAACLGKLTDCCQSLSGEAGEPCVIHGLTNPDGGFYVLCQGDSENPSISDDVIGQCW
                     VWRSKNGALVFDSIEMPKDKLMMYKNIVVDFYQSLGKKLVQEGYTHKVNCGKNSGISA
                     DVGFATLEYNKEEFIDYQGYCDSRVQFSIFDEDFPYPHYVKDSVITQKIDEDLEKAMR
                     SNMPLVQVKEFANALNWAFVEKYNRNMNTNADAIIDKIQELSIQYNKQE"
     gene            1000..2379
                     /locus_tag="VFP93_03505"
     CDS             1000..2379
                     /locus_tag="VFP93_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843706.1"
                     /translation="MDKFVKRSFDFDELILLIKENKVHIDTFDGVNKTLLMLAANLGN
                     DAIVQELIKFGAKIDLRNNECHTALMQATQGNCSNENEYSLISASPKSSINNVCKILI
                     AQGADVNTKDQYGDSVLMHACHKGLGDHVDLLLAHGADVNYENNNNTSLIFAARGGFD
                     QIILKLLDKGANMHHKNALGCTALSEAAANGFEGTVNILKQRGADINSQDNDGNTPLL
                     RSIEGGYKCVINTLLTSNADVHIKNNSGDTVLMASIRQRINETLIEQILEKKPDLDAK
                     ARNNNTALGYAIDTKNEKLALTLIALGACPNVKHNGYPLLVKAIKNNLNCVIEKLIGK
                     VDLNAKVIIDNVYLNNSRSNDDNALLSAVASKNILLVEKLVKFGADINAVGYLGRSAL
                     MDAIASYNTEMALFLISLGSNLEVKDKNGRTALDYAARYGDENVKDKINEINAQSEEK
                     RNLLKIRMR"
BASE COUNT          872 a          378 c          510 g          730 t
ORIGIN      
        1 caacttttaa gtcttgatct attgaatttg aacgatgagc ttgtggaatt atgttggaat
       61 atttctttag aaaacgcaga tattcaaatt ttgaaagctt tccatgacta ttatgtaaaa
      121 tccaccacgc cagcttttca atatttttta aaaaatggcc ttacgaagaa aaattatgac
      181 aaatttctgt tactagacag agataatgca ggcgagaata tcccagaaat tactatagaa
      241 ggtgcagaaa ttaaacatcc tggattttat cttaagaaag tatgcgctaa aaatgatcaa
      301 gaggcagcac gagccgcttg tttgggcaaa ttaaccgatt gttgtcagtc tttatcgggg
      361 gaagccggtg aaccttgtgt aatacacggc ttaacaaatc ccgatggtgg tttttatgtt
      421 ctgtgccaag gagacagcga aaatccaagt atatcggatg acgtaatcgg acaatgctgg
      481 gtttggcgca gtaagaacgg ggcgcttgtt tttgactcca tcgaaatgcc aaaagacaaa
      541 ctaatgatgt ataaaaatat tgttgtggat ttttatcaat ctttagggaa aaaattagtt
      601 caagaaggtt acacacataa agtaaattgt ggtaaaaatt caggcatttc tgcggatgtt
      661 ggatttgcaa ctttagagta taacaaagaa gaatttatag attaccaagg atattgtgat
      721 tcacgagtcc agttttccat ttttgatgaa gatttcccct atccacatta tgttaaagat
      781 tccgtaatca cccaaaaaat cgatgaagat ttagaaaagg caatgcgatc caacatgccc
      841 ttagtgcaag tcaaagaatt cgcaaatgct ttgaattggg cctttgtaga aaaatacaac
      901 aggaacatga atactaacgc cgacgcaata attgataaaa ttcaagaact ctccatccaa
      961 tataacaaac aggaataagt tgcggaatta atttccatta tggataaatt tgtaaaaaga
     1021 tcgtttgact ttgatgaatt aattctatta ataaaagaaa ataaggtgca tatcgatact
     1081 tttgatggtg ttaataaaac tttgctaatg cttgcagcaa atttgggtaa tgatgcgatc
     1141 gtgcaagaat taattaaatt cggagccaag attgatttgc gtaataatga atgtcacaca
     1201 gctttgatgc aagcgacaca agggaattgc agtaacgaaa atgagtatag cttaatttca
     1261 gcaagtccga aaagttctat taataatgta tgtaaaatat tgatagcaca gggtgccgat
     1321 gttaatacaa aagatcaata tggcgatagt gtgttaatgc atgcgtgcca caaaggactt
     1381 ggcgatcatg ttgacctatt attagcgcat ggtgcagatg ttaattatga gaataacaac
     1441 aacaccagcc tcatttttgc cgctcgagga ggctttgatc aaattatttt gaagcttttg
     1501 gataaaggtg cgaatatgca tcacaagaat gctctgggtt gcactgcgct ttcagaggct
     1561 gctgcaaacg gatttgaggg tacagtaaat attctcaagc agagaggtgc agatattaat
     1621 tcccaagata atgatggcaa taccccttta ttaagatcaa tagagggtgg ttataagtgc
     1681 gtaattaata cattattgac aagtaatgca gatgtccata taaaaaataa tagcggtgac
     1741 acagtattaa tggcatccat tcgacaaagg ataaacgaaa ctttaattga acaaattctt
     1801 gagaaaaaac cagatcttga tgcaaaggct cgcaataaca atacggcttt aggttatgcg
     1861 attgatacca aaaatgaaaa gttagctcta acattaattg ctttaggtgc atgccctaac
     1921 gtaaaacata atggctatcc gttacttgta aaagctatta aaaataattt gaattgtgta
     1981 atcgaaaaat taataggcaa ggttgactta aacgcaaagg ttattattga taacgtctat
     2041 ttgaataata gtcggtcgaa tgacgataac gctttgctat cagcagtcgc gtctaaaaac
     2101 attttgcttg tcgagaagct tgtcaaattt ggagcagata tcaatgcagt gggctattta
     2161 ggtagaagtg ctttaatgga cgcgatagca tcttataata cagaaatggc tttattcttg
     2221 attagccttg ggtcgaattt ggaagtaaag gataaaaatg gtagaactgc gttagactat
     2281 gctgcgcgct atggagatga aaatgttaaa gataaaatca atgaaataaa tgctcaatcc
     2341 gaagagaaac gcaatttact aaagatcaga atgcgataat ttagtgtaaa cgttatcgca
     2401 tcgaactcgt gcgcgtttga attaaaaaaa ttgagattct cacgcgctct ttcgcaaaaa
     2461 tggaatgaag atttaatgtg gaagtgtttt
//
LOCUS       DASTIB010000206         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_480056, whole genome shotgun sequence.
ACCESSION   DASTIB010000206 DASTIB010000000
VERSION     DASTIB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480056"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFP93_03510"
     CDS             complement(<1..545)
                     /locus_tag="VFP93_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843707.1"
                     /translation="MLTYFKKMLAISVLGSLCIVQAHALTLKPHIGADYKFWEIKEKQ
                     LDFTRDYFSIFPYMSRAGTLYVGTRINKIWGLDFGYDRSVRKDEFHSYDSPGALTPVF
                     GDTPVLGDAIQTDFRIEAWHLSGLFYWEILKNFELVFHIGVASLQPKSTVLYYHAALP
                     NDPIDLRFQLKSKWVGRFGFGA"
     gene            complement(665..1885)
                     /locus_tag="VFP93_03515"
     CDS             complement(665..1885)
                     /locus_tag="VFP93_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9843708.1"
                     /translation="MKKIKSVIFKFILLFFVFVLTGCDGEKTYNFIPNFSNLNGKKFF
                     QPLPQNKLRYGLFNLKDNAPLGERELEFEKVTTPQALWTRMRTGFKLNYAESNPKIQK
                     FIREYSKNSDYVNRLSHNASPYLFFIVEELVKRNLPLEIALLPMIESDFNPFALSEKG
                     AHGMWQIMPGTAKILGLKQDAWYDGRRDITASTRAALNHLQYLNRTFQGDWFLTLAAY
                     NAGERRIIRAIENNKNHQKPTDYWHLKLPQQTLEYVPKLLAFVSIVKNPTKYGVQLPS
                     IANQPYLQTIDLDQQIDLKLVAHLSNTPLKEIKRLNPALQQHKTPPSGPHRIVVPIAI
                     ADHFQKIIKKTDPTVLANLKSYEIKDGDDLIKIAALFNADVEKIKELNHLISNKIDSG
                     QTLFIPPDTKVKIS"
     gene            complement(1996..2769)
                     /gene="gloB"
                     /locus_tag="VFP93_03520"
     CDS             complement(1996..2769)
                     /gene="gloB"
                     /locus_tag="VFP93_03520"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HET9843709.1"
                     /translation="MVSIHPIRALKDNYIWLLRSPKQYACVFDPGESAPVLTFLQKNK
                     LKLLSILITHHHWDHVSGVLELLQHYPNITVYGPAHDAINGLNNPVSEPNEIYLPELE
                     LSFKVLSIPGHTLDHIAYYNNKFLFCGDTLFSAGCGRVFEGTMPQMLQSLEKLKALDG
                     DILMFCAHEYTKKNLEFALTLEPENLAIKKRLCQVKEQLAQEKPSLPVRLREEKQTNP
                     FLRCREAKLIQAVIKKDPRIDPKDPIAVFTALRKWKDTY"
     gene            2820..3560
                     /locus_tag="VFP93_03525"
     CDS             2820..3560
                     /locus_tag="VFP93_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9843710.1"
                     /translation="MNPLEPWWFTTKAGKIFLNDEKDNLNTLLPSLYGYHLIQCAPPF
                     LGSFVSSSLISHRVLINIHGSSNWPCSLIRSEMECLPLLNDSVDVVLLTHTLELVSRP
                     HQMLREAHRALIAQGHVVITGLNPFSFWGILILIKRIFSRSPPYKMISPNRVKDWLKL
                     LDFEIKEYKMFYYRPPFSNVKFMNRLQFFEKMGQRFWPFFGGGYVIVAVKKVLRFTPV
                     RPRFIRPKKIWDAQGVTGSATYKKEGPM"
     gene            3557..>3978
                     /gene="rnhA"
                     /locus_tag="VFP93_03530"
     CDS             3557..>3978
                     /gene="rnhA"
                     /locus_tag="VFP93_03530"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET9843711.1"
                     /translation="MKKRVAIFTDGACSGNPGPGGFGVILRYGKHEKTLSGAEPMTTN
                     NRMELMAAIVGLRSLKESCYVDITTDSKYVKDGVTKWLAKWQQNGWRTSNKKPVKNQD
                     LWQQLILELHKHHIEWHWVKGHSNHPENERADLLARTA"
BASE COUNT         1207 a          720 c          789 g         1262 t
ORIGIN      
        1 gccccaaagc caaagcgacc aacccatttg gattttaatt gaaaacgtag atctataggg
       61 tcattgggca gagctgcgtg ataataaagt acggtacttt ttggttgtaa ggaagcgaca
      121 cctatatgaa aaactagctc aaaatttttg agaatttccc aataaaataa tccacttaaa
      181 tgccaagctt caatacgaaa atcggtttga atggcatctc caagaacggg tgtatcccca
      241 aataccggcg ttagggctcc tggggagtcg taactatgaa attcatcttt tcgaaccgat
      301 cgatcgtatc caaagtctaa accccaaatt ttgttgatgc gggtgccaac atataaagtg
      361 ccggcacgcg acatatacgg aaaaatgctg aaataatcgc gcgtgaaatc gagttgcttt
      421 tcttttattt cccaaaattt atagtcggcg ccaatatgtg gtttgagggt taaggcatgt
      481 gcttgaacta tacataaact ccctaggaca gagattgcga gcattttttt gaaataagtt
      541 aacacggtgc cttccttgct ggcgtggtta tttctatttt gtacagctaa aacccaagcg
      601 tcaactaaaa ttcacggaat tatattggat aaagcgagca aaataccaaa cttcgtggtg
      661 accattacga aattttgact ttggtatctg gcggtataaa cagcgtttgt ccagaatcga
      721 ttttgttcga tattaagtga tttaattctt tgattttttc tacatcagca ttaaagaggg
      781 ctgctatttt tattaaatca tcgccatctt taatttcata actttttaga ttcgcaagaa
      841 ctgtaggatc cgtttttttt attatttttt gaaaatgatc ggctatcgct attggaacca
      901 ctatgcgatg cggaccgctg ggtggcgttt tgtgttgttg cagcgcagga ttaagacgct
      961 taatttcttt taacggcgta ttcgaaagat gggcgactaa ttttagatca atctgttgat
     1021 ccagatcgat agtttgtaga taaggctgat ttgcaatact tggcagttgt acgccatatt
     1081 ttgtgggatt ttttacaatg gaaacaaatg ctaataattt tggcacatat tccaaagttt
     1141 gttgaggcaa tttgagatgc caatagtcgg tgggtttttg atgattttta ttattttcaa
     1201 ttgcacgaat aattctgcgc tcgcccgcat tataggcagc caaggttaga aaccaatcac
     1261 cttgaaaggt acggtttagg tattgtaagt ggttcagagc tgcgcgcgtg gaagcggtga
     1321 tatctcgacg tccatcatac caagcgtctt gtttaaggcc taagattttt gcagttcctg
     1381 gcataatttg ccacataccg tgcgcgcctt tttcactgag cgcgaaggga ttaaaatcac
     1441 tttcgatcat gggtaacaaa gcaatttcta aaggtaaatt tctttttacc aattcttcga
     1501 caatgaagaa taaataaggg cttgcattat gcgaaagtcg atttacataa tcgctatttt
     1561 ttgaatattc tcgaataaat ttttgtattt ttgggttaga ttctgcatag tttaatttga
     1621 atccggtgcg cattcttgtc caaagcgctt ggggtgtggt aactttctca aattcaagct
     1681 cgcgctcacc cagcggtgca ttatccttta aattaaataa accgtagcgt aacttgtttt
     1741 gcggtaaagg ttgaaaaaat tttttaccat tcaaatttga aaaatttgga atgaaattat
     1801 aggttttctc tccatcacaa cctgttaaga caaatacaaa aaataacaaa ataaatttga
     1861 aaataaccga ctttattttt ttcattctaa aatgctcgta ctaaaagttg actatcctca
     1921 aataaaaaag cctaacaata atgagttggt ctgcataata attcaatagc aactcctttg
     1981 caaatttttt tgtagttagt aagtatcctt ccacttgcga agggcagtga atacagcaat
     2041 gggatcctta ggatcgatac gaggatcttt tttaattacg gcttgaatta acttagcttc
     2101 tctacaacga agaaaaggat tggtttgttt ttcttcgcgc aaacgcaccg gcaagcttgg
     2161 tttttcctgc gccaattgtt ccttgacttg acataacctt ttttttatcg ctaaattttc
     2221 tggttcaagt gttaaagcga actctaaatt tttttttgta tattcatggg cgcaaaacat
     2281 taaaatatca ccatctagcg cttttaattt ttctagagat tgcagcattt gaggcatggt
     2341 accttcaaac acgcgcccac aacctgctga aaataaggtg tctccacaaa ataaaaattt
     2401 gttgttgtaa taagcaatat gatctagagt atgtccaggt atgcttaata ctttgaaaga
     2461 taactcaagt tctggcaaat aaatttcatt tggctcagat actggattat tcaatccatt
     2521 aatggcatcg tgagctggac catacaccgt gatatttgga taatgttgaa gcaactcaag
     2581 gactccgctt acatgatccc aatgatggtg cgtaattagt atactaagta actttaattt
     2641 gtttttttgt agaaaagtta agacaggagc tgattcacct gggtcgaaga cacaagcata
     2701 ttgctttgga cttctcaaaa gccaaatata gttatctttt aaagctcgaa tcggatgtat
     2761 ggaaaccata caatttaaag taaacgaaac gataccttaa ggcaagagaa atagacgacg
     2821 tgaatccatt agaaccttgg tggtttacca caaaagcggg aaaaattttt ctcaacgatg
     2881 aaaaagataa cttaaataca ctcctgccaa gcctctatgg ttatcactta atacagtgtg
     2941 cacctccttt tttgggttca tttgtaagtt caagcttaat atcgcatcgc gtgttaatta
     3001 atatccatgg ttccagtaat tggccatgtt ctttaattcg ttccgaaatg gaatgtctcc
     3061 ccctccttaa cgatagcgta gatgtggtat tacttaccca tactttagaa ctcgtgtcac
     3121 gcccgcatca gatgttacgt gaagcacatc gcgcattgat cgcgcaagga cacgttgtaa
     3181 tcaccggatt aaaccctttt agtttttggg gcatattaat tttaataaag cgtatttttt
     3241 cccgctctcc accctataaa atgataagcc ccaatcgtgt gaaagattgg ctaaaattac
     3301 tcgattttga aattaaagaa tataaaatgt tttattatcg tcctccgttt tcgaatgtaa
     3361 aatttatgaa ccggttgcaa ttttttgaaa aaatgggtca acgtttttgg cctttttttg
     3421 ggggcggtta tgtcattgta gcggttaaaa aagtgttacg ttttactcct gttcgtccaa
     3481 gatttatccg accgaaaaaa atttgggacg cacaaggtgt tacgggcagt gcaacgtata
     3541 aaaaagaagg tccaatgtga aaaaacgtgt cgctattttt acagatggcg cgtgttcggg
     3601 taatccaggc cctggaggct ttggtgttat tttacggtat ggaaaacatg aaaaaacgct
     3661 aagtggtgcc gaaccgatga caaccaataa tcgcatggaa ttaatggcag ctattgttgg
     3721 gttgcgttcg ttaaaagagt cttgctacgt ggatattaca acggactcta aatatgtaaa
     3781 agatggcgtt acaaaatggt tagcaaagtg gcaacaaaat ggttggcgca catccaataa
     3841 aaaaccggta aaaaatcaag atttatggca acaattaatt ttagaattgc acaaacatca
     3901 tattgaatgg cattgggtca aaggccattc taaccatccg gaaaatgaac gtgcagattt
     3961 gctggcgcga acagccat
//
LOCUS       DASTIB010000207         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1325, whole genome shotgun sequence.
ACCESSION   DASTIB010000207 DASTIB010000000
VERSION     DASTIB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..735)
                     /locus_tag="VFP93_03535"
     CDS             complement(130..735)
                     /locus_tag="VFP93_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9843712.1"
                     /translation="MLSEINLTVEAGETLCIMGASGSGKSTLLHILGLLDTPSSGTYI
                     FNNQNLRLMSKKEKNILRNKNIGFLFQSYHLLKGWSVLDNITLPLLYRNVPLHEARHT
                     AKTLLTHMNLTDLMNQKTQVLSGGQKQRVALARTLSIQPKLLLLDEPTGALDYENAQC
                     MMKLVFDLQVLWGFTLVMVSHDPTYANYCNKSFQLLAPLCN"
     gene            complement(773..1951)
                     /locus_tag="VFP93_03540"
     CDS             complement(773..1951)
                     /locus_tag="VFP93_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843713.1"
                     /translation="MWSKFFFPILILIIYPAFGNLTLYEVKKESIPEIFEIMGTWVPI
                     TWEPIMVPFPGVLKKIHCPFGSNVKKGDKLFELQDEELPLKIQEAHLAYQNKMQAYEK
                     LLHWSTSPEYLQAKRNLQLESQNLDEKRRHLQETETLVVMGGLAKEVLLQEQRDFAYL
                     KLRLSELQQRFDIIQKKGDSLALAQANIDATYAKMHFDKLKHQEQQLMQRAELDGTLY
                     SANLFQTGQDYRHVKEGMRIESDQWLGVLTSVDSFGATFEVSENWVDKIEQAGTVHLE
                     VKGELLAAKVYSIEPVGLSSQTPPKYIVKIQPIKSPRIRVKIGTFVKLQISNTLKHTG
                     IKIPLQLVFWDKNQPFVYKWNATKKHMQKHFVGLGALNPPWIEVTTGLLEGDKIGSQA
                     "
     gene            complement(1941..>2615)
                     /locus_tag="VFP93_03545"
     CDS             complement(1941..>2615)
                     /locus_tag="VFP93_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9843714.1"
                     /translation="LKEDAPEPISLSISAIPLEDALIEKALHYHLAPLLEELTHAKED
                     ERRLRDNFLWDIRLKGSWSIDKQRYFWMEDILNQEVYVKSRGSNHRYLGLFMTIPLSK
                     DYSTHIALYQNKVIQEKKHLDLKHKELMLKQEIHNLFQQFQQWQNQQSIVEKAFSFAQ
                     HMLRIEEEKYLAGRSSLLDLQRMQEIEMQSALDKISLQATLLLARVELDYCIGLLPNT
                     WREYVV"
BASE COUNT          758 a          508 c          464 g          885 t
ORIGIN      
        1 tcgaggcaaa ttcttttaaa ttggataatg tgcttaatgc cgtgatacca acccccataa
       61 gcacaataga aaaaatgctc cgaaggggtt gcagccaaag actacgcaaa ctatcccaaa
      121 tagttatctt tagttgcata ggggtgcaag taattgaaaa cttttattac aatagttggc
      181 atatgttgga tcgtgtgaca ccatcacaag cgtaaatccc cataacactt ggagatcaaa
      241 aacaagtttc atcatgcatt gtgcgttttc ataatctaat gcccctgttg gttcatctaa
      301 aagtaataat ttaggttgaa tactcaaggt gcgagccaaa gctacccgtt gtttttgtcc
      361 acctgacaac acttgggttt tttgattcat taaatcggtt agattcatgt gggttaacaa
      421 agtctttgcg gtgtgtcttg cctcatgtaa gggtacgttg cgatacaaaa gcggtaaggt
      481 gatattatcg agcacactcc aacccttgag tagatggtac gattgaaata aaaatccaat
      541 atttttatta cgtaaaatat ttttttcctt tttagacatt aaacgtaaat tttgattgtt
      601 gaaaatataa gtaccagagc tcggcgtatc taagagtcct aaaatatgca gcaacgtcga
      661 tttaccggaa ccactagcac ccataataca caatgtttca cccgcttcca cagttaaatt
      721 aatttcagaa agcacaagct tttttccgaa atacttcata atagtgtaca attcaagctt
      781 gactcccaat tttatctccc tctaataatc ctgtggttac ctcaatccaa ggggggttta
      841 gggctcctaa accaacaaag tgtttttgca tgtgtttttt tgtggcattc cacttataaa
      901 caaatggctg atttttatcc caaaatacca attgtaaagg tatttttatc cctgtatgtt
      961 tgagagtatt gcttatttga agttttacga atgtacctat ttttacccta attctgggtg
     1021 atttaatagg ttgaattttt acaatgtatt ttggtggcgt ttgtgaactt aaaccgacgg
     1081 gttcaatact atatactttt gctgcaagaa gttcaccttt cacttctaaa tgcacagttc
     1141 ccgcttgttc aattttatca acccaatttt cggatacttc gaatgtggca ccaaaagaat
     1201 cgaccgacgt gagaacgcct aaccactgat cggactcgat acgcatacct tctttgacgt
     1261 gtcgatagtc ttggccggtt tgaaaaagat tcgcagagta taaagtgccg tctaattctg
     1321 cacgttgcat gagttgttgt tcttggtgtt ttaacttgtc aaaatgcatt ttagcatacg
     1381 tagcatcgat gtttgcttgc gctaacgcca aagaatcccc ctttttttgg ataatatcaa
     1441 aacgttgttg caattcacta agacgcagtt ttaagtatgc aaagtcgcgc tgttcttgaa
     1501 gaagtacttc ttttgctaag cctcccatta ctactaacgt ttctgtttct tgtagatgtc
     1561 gcctcttttc gtccaaattt tgcgattcta actgtaaatt tcttttggct tgcaaatatt
     1621 cagggcttgt tgaccaatgc aatagttttt cataagcttg cattttattc tggtacgcta
     1681 aatgtgcttc ttgaattttt aaaggtaatt cttcgtcttg caactcaaaa agtttatccc
     1741 ctttttttac attactgcca aaaggacaat gtattttttt taaaacgccg ggaaatggga
     1801 ccatgatggg ttcccacgta atgggcaccc atgtacccat gatttcaaat atttctggta
     1861 tagattcttt tttaacttca tacaacgtga gattgccaaa cgcgggataa ataatcaata
     1921 ttaaaatggg gaagaaaaat ttagaccaca tattcccgcc atgtattcgg caataatcct
     1981 atgcaataat ctagctcaac cctagctaat aacaaagttg cttgcaaaga aattttgtct
     2041 aatgctgatt gcatctctat ttcttgcatt ctttgtaaat cgagtaaact cgatcgaccg
     2101 gcaagatact tttcttcctc aatccgtaac atatgctgag caaaagaaaa tgccttttcc
     2161 acaatggatt gttgattttg ccattgctga aattgctgaa agagattgtg aatttcttgt
     2221 ttaagcataa gctctttatg ttttaaatca agatgttttt tttcttgaat gactttgttc
     2281 tgatataatg caatatgggt gctatagtcc ttggaaagcg gtatcgtcat aaacaatcca
     2341 agatatcggt gattactgcc ccgtgatttc acataaactt cttggtttaa aatatcttcc
     2401 atccaaaagt aacgctgctt atcgatactc caactgcctt ttaatcgaat atcccataaa
     2461 aaattgtcgc gcaaacgtcg ctcatcttct tttgcgtgcg ttaattcttc taggagaggc
     2521 gcgagatgat aatgaagagc tttttcaatt aacgcatctt ctaaaggaat ggcacttata
     2581 cttaacgata tgggttctgg agcatcttct ttcaa
//
LOCUS       DASTIB010000208         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1250473, whole genome shotgun sequence.
ACCESSION   DASTIB010000208 DASTIB010000000
VERSION     DASTIB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250473"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFP93_03550"
     CDS             <1..1098
                     /locus_tag="VFP93_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843715.1"
                     /translation="QYNDALFHARTILEENITDEKFADYAHKVYFLSDGEPTANHSAL
                     DNQEWPEQWDSWQEFIQNPQSTIPNSFAQGIDAFAVSIGNSSKIEQALEPIVLDPKYV
                     FSAQIDLANLGEELLRTVPDYFEANLLHNDIDNNHPRLVNEISFKVEDATQYMLDHNL
                     YGTGAFASVDNTTVIIPVPPVDSYVEFPTPLGATCLLNPHGEIFYLPPKVEEDTFEIF
                     EYYAIDLTNNNVDSAKVYFHVLSNHEKLHYVTGNEPLTNRGLEDVIILEGKEGDHTFI
                     IDLENAKPQTIVIEDLFQNNNVLHFTHTQSVRLEELFTDFTVHGTLGPLEFTLADGPT
                     TLMLNNPGAYVGDNLLEALRFLEQHVQVDIL"
     gene            complement(1100..>2778)
                     /locus_tag="VFP93_03555"
     CDS             complement(1100..>2778)
                     /locus_tag="VFP93_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9843716.1"
                     /translation="WLKNYQAGVNSEIDPSQYHSLVEYAESCFNEFNDRTAYVNMGKS
                     ISYQALDHLSQCFANYLRCELKLKKGDRVAIMLPNVLQYPVAMVGIHRAGLVVVNLNP
                     LYTARELEYVLNDCEASTIIVLTNFAHTLQKALPKTQIKHIILTQIGDLLGTIKGNII
                     NFVVKNFKKMVPNYHLPQAEHFNKVLTLGKRHTYQKPNLTHEDIAYLQYTGGTTGRAK
                     GAILTHRNMVANVLQATEWINPLVQEKLEGGIITALPLYHIFSLTANCLTFLKVGIPN
                     ILITNPRDIPGFVKELKKHPFAVITGVNTLFNALLHNKDFASCDFSKFRFALGGGMAV
                     QKAVAQKWQKVTGIPLVEAYGLTEASPAVTINPMDTKEFNGSIGLPIPSTEISIRDDH
                     ENELPIGESGELCVKGPQVMRGYWNDEEKTKLVFTEDGWLKTGDIATIDAQGYIRIVD
                     RKKDMITISGFKVYPNEVEDVIASIPGVKEVAIIGIPDSEHGERVRAYVVKSDETLTE
                     QTILTTCKENLTHYKIPKEIVFRAELPKTNVGKVLRRALREDILREHSLS"
BASE COUNT          821 a          555 c          523 g          879 t
ORIGIN      
        1 caatataacg atgcactttt tcacgcgcgt accatacttg aagaaaatat taccgatgaa
       61 aagtttgcag attatgcaca caaagtttac tttttatcgg atggagagcc tacggccaac
      121 catagtgcgc tggacaacca agagtggccc gaacaatggg actcctggca agagtttatt
      181 caaaatccac aaagcacgat tcccaatagc tttgcgcagg gtattgatgc atttgcagta
      241 agtattggca acagtagcaa aattgaacaa gcattagagc ctattgtttt ggatccgaaa
      301 tacgtatttt ccgcacaaat agacttagcc aatcttggtg aggagctctt gcgcacggtt
      361 cccgattatt ttgaagcgaa tttattacat aacgatattg ataacaacca ccctcgcctt
      421 gttaatgaaa tttctttcaa ggtagaagat gcaactcaat atatgttaga tcataacctc
      481 tatgggactg gggcttttgc aagcgttgac aatactaccg tcattattcc agtgcccccg
      541 gtagatagtt atgtagaatt tccaacgcct ttaggtgcta cctgtttgct taatccgcat
      601 ggtgagatat tctacctgcc acctaaagta gaagaggata cttttgaaat ttttgaatat
      661 tatgcaattg atcttaccaa taataatgtc gatagtgcta aagtatattt tcacgtactc
      721 tcaaaccatg aaaaattaca ttatgtaaca ggcaatgaac cgctcaccaa tcgtggttta
      781 gaagatgtga taattttgga aggaaaagaa ggggatcaca cttttattat cgatctagaa
      841 aatgctaaac cacaaaccat cgtgattgaa gatttatttc aaaacaacaa cgtcctgcat
      901 tttacccata cccaatcagt gcgcttagaa gagttattta ccgattttac agtacacgga
      961 acattgggac ctttggaatt cacgttagca gatggcccta caacactcat gttaaataat
     1021 cctggagcct atgtaggtga caatctactt gaggctttac gatttttaga gcaacatgta
     1081 caggtagata ttctttaaat tagcttaaag aatgttcgcg caagatgtct tctctcaatg
     1141 cgcgtcgtaa aacttttcct acattcgttt tcggtaactc ggctcgaaat acaatttcct
     1201 tcggtatttt atagtgggtt aaattttctt tacatgttgt taggatggtt tgctcagtta
     1261 aggtttcatc ggattttacg acataggcac gcacgcgttc tccatgctca gaatcgggta
     1321 tgccaataat tgctacttct ttgacgcctg gaatggatgc aatgacatct tctacttcgt
     1381 ttggatacac tttaaaaccg gagatcgtaa tcatatcttt tttgcgatcc acgatgcgga
     1441 tataaccttg tgcatcgatc gttgctatat cccctgtttt aagccaacca tcttccgtaa
     1501 ataccaattt agttttttct tcgtcgttcc agtaacctcg cataacttgt ggtcctttaa
     1561 cacacaattc acccgattca ccgatgggaa gctcgttttc atgatcatcg cgtatgctaa
     1621 tttctgtaga gggtatgggt aaaccgatac tgccattaaa ttcttttgta tccatcggat
     1681 ttatcgtaac tgcgggactc gcttccgtta acccataggc ttcaaccaaa ggaattcctg
     1741 ttactttttg ccacttttgc gctacagctt tttgtacggc catcccgcca ccgagggcaa
     1801 aacgaaattt agaaaaatca cagctagcaa aatccttatt atgcagcaat gcattaaaca
     1861 aggtgtttac gcccgttatt actgcaaacg gatgtttttt taattctttc acaaatcctg
     1921 gaatatcccg cggattggta atgagtatgt tgggaatccc tacctttaaa aaagttaaac
     1981 aattggcggt gagagaaaaa atatgataaa gcggcaacgc ggtgataatg cccccttcta
     2041 atttctcttg cactaaaggg tttatccatt ctgttgcttg taacacattg gctaccatat
     2101 tacgatgcgt taaaatagca ccttttgctc tacctgtagt accacccgta tattgcaaat
     2161 aagctatatc ttcgtgagtt aaattgggct tttggtaggt atgtcgcttt cccaatgtga
     2221 ggactttgtt aaaatgttct gcttgtggta aatgataatt gggcaccatt tttttgaaat
     2281 ttttaacaac aaaattgatt atgtttcctt tgatagtgcc caataaatca ccaatttggg
     2341 ttaagatgat atgtttaatt tgggttttag gtagtgcttt ttgtaaagta tgggcaaaat
     2401 tcgtaagcac aataatggta gatgcttcac aatcattcaa cacgtattct aattctcgtg
     2461 cggtatataa aggatttaaa ttgacaacca ctaatcctgc acgatgaatt ccaaccatgg
     2521 caacaggata ttgcaataca ttggggagca tgattgctac cctatcgcct tttttaagtt
     2581 ttaactcgca acgcaaataa ttggcaaaac attgagacaa atggtctaac gcttgataag
     2641 aaatggactt gcccatgtta acgtaagcag tacgatcgtt aaattcgtta aaacaacttt
     2701 cggcgtattc tactaaagaa tggtattggc tgggatcaat ttcactatta acaccagctt
     2761 gataattttt taaccaaa
//
LOCUS       DASTIB010000209         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_708650, whole genome shotgun sequence.
ACCESSION   DASTIB010000209 DASTIB010000000
VERSION     DASTIB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708650"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1095
                     /gene="hflC"
                     /locus_tag="VFP93_03560"
     CDS             139..1095
                     /gene="hflC"
                     /locus_tag="VFP93_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014980558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET9843717.1"
                     /translation="MNQTKTLTLVLGILIAVFLFLSVFIVDQTERAIVLRLGQMQDDV
                     EGKIRILGPGLHFKVPFIDTVRFFDKRIQTLQIESSRIPTEEKKELIIDLFNKWRIKD
                     FSKFFRATGGNTRKAVQLLTEKTNDGLRAEIGRRQLNDVVSEDRDLIMAKISKDVNAA
                     AKTLGIEVVDARMVRIDLPREVSEAVFGLMRTERKRIAAEHRARGRSRAEAIQANADA
                     KVTVILARAHQEGRETRGQGDASAAKIYEASYGKNPEFFRFYRSLEAYKRTFQTKNDM
                     LVIKPDSDFFKYFQNIQGSSRNGNGNGNGNGNGNNGKKPENS"
     gene            1115..1309
                     /locus_tag="VFP93_03565"
     CDS             1115..1309
                     /locus_tag="VFP93_03565"
                     /inference="COORDINATES: protein motif:HMM:NF021366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HET9843718.1"
                     /translation="MSIAWQDVWAALALLFVFEGMMPFINPVRWKTMMQRISQQSDHS
                     LRIMGLTSMLGGVLILYFVR"
     gene            1317..>2591
                     /locus_tag="VFP93_03570"
     CDS             1317..>2591
                     /locus_tag="VFP93_03570"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9843719.1"
                     /translation="MGKSIVILGSQWGDEGKGKIVDLLTANAHAVVRYQGGHNAGHTL
                     VICGEKTVLHLIPSGILHPKVKCYLGQGVVISPKALLEEIQTLNLKGFDVENRLGISP
                     AATLILPYHIALDKAHESHKGIHAIGTTGRGIGPAYEDKVGRRSLRVSDLLNMDLFKD
                     KLEKVLAYHNCLLTQFYQEAPVSLELVLNEVYQTRETLLSLLVDVRGELEVLRSKGAN
                     IIFEGAQGALLDIDHGSYPFVTSSNTVAGAVTTGAGFGPLYIDEVIGVTKAYCTRVGS
                     GPFPTELSDATGELLRSRGQEYGATTGRARRCGWFDVCLMRHSAFINSISHCVITKLD
                     VLDECDEIKICTHYRLGEKLLTHPPVSSEDFGKCEPIYETLPGWKTSTLGVIDYRDLP
                     QNAQKYLERIAFLAKIKISMISTGAERAHNIKL"
BASE COUNT          806 a          450 c          615 g          720 t
ORIGIN      
        1 aaccgtcgat ccaaatcgca atatcacgga attaactata gacaaaaccc agctccctga
       61 ggatggcaat accataagac ctgctcggga acgatatgaa gacatcagca gatatcgtaa
      121 tatgagaggg ggttaaaaat gaatcaaaca aaaacattaa cgctagtact aggaatttta
      181 attgcggttt ttttattttt gagcgttttt attgtagatc aaacagaacg tgcgatcgtg
      241 ttacgtcttg gacaaatgca agacgatgtc gaaggaaaaa tacggatctt aggaccaggt
      301 ttacatttta aagtgccatt tatcgatacg gtgcgctttt ttgataagcg aattcaaacc
      361 ttacaaatag aatcttccag aattcctacc gaagaaaaaa aagaacttat aatcgattta
      421 tttaataagt ggcgtattaa agatttttcg aaattctttc gtgccacggg cgggaataca
      481 cgcaaagcgg ttcaactatt aaccgagaaa acgaatgatg gattgcgtgc tgaaattggt
      541 cgacgtcaat taaacgatgt ggtttcggaa gatcgtgatc tcatcatggc gaagattagc
      601 aaagacgtga atgcagcagc caaaactttg gggatcgaag tagtggatgc taggatggtt
      661 cggatagatt tacctcgcga agtaagtgag gcggtatttg gattaatgcg taccgaacgt
      721 aaacgtatag cagcggaaca tagagcaaga ggacgttccc gagctgaagc aattcaggca
      781 aatgcagatg ctaaggttac tgtgattttg gcacgcgcac atcaagaggg tagagaaaca
      841 cgtggtcaag gcgatgcatc agctgcaaaa atttatgaag cctcttatgg taaaaaccct
      901 gaattttttc gattttatag aagtttggaa gcgtacaaac gtacttttca gacaaaaaat
      961 gatatgctag taatcaagcc agatagcgac ttctttaaat atttccaaaa tattcaagga
     1021 tcttcgcgaa acggaaacgg aaacggcaat ggcaatggaa atggaaataa tggtaaaaaa
     1081 ccagaaaaca gttaattaaa attaggtagt aaatatgtcg atcgcttggc aagatgtttg
     1141 ggctgcgctc gcattgttgt ttgtttttga agggatgatg ccatttataa acccagttag
     1201 gtggaagaca atgatgcaaa gaatttcaca acaatcggat cattctttgc gtattatggg
     1261 tttgaccagc atgctgggcg gtgtcttaat attgtacttt gtacgttagg caaattatgg
     1321 gcaaaagtat cgttatcctg ggtagtcagt ggggcgatga aggtaaagga aaaattgttg
     1381 atcttctcac cgcgaatgcg cacgcggttg tacgctatca aggtggtcat aatgccggtc
     1441 acaccttggt aatttgtgga gaaaagacgg ttctgcacct gatcccatca ggaattttgc
     1501 atcctaaggt caaatgctat ctaggtcagg gtgttgttat ttcacctaag gcattattag
     1561 aagaaattca aactttgaat ttaaaagggt ttgatgtaga gaataggctg ggtatcagcc
     1621 ctgctgcaac tttgatcctg ccgtaccata tagcgcttga taaggcacat gaaagccaca
     1681 aaggaattca tgctatcgga acaacaggac gtggaattgg ccctgcttat gaagataaag
     1741 ttggtcgccg tagcctgaga gtaagtgatc ttttaaatat ggacctattt aaagacaagc
     1801 ttgagaaagt actggcttat cacaattgcc ttttaacgca attttatcaa gaagctccag
     1861 tgtcattgga gttagtgtta aacgaagtat accaaacgcg tgaaacattg ctttctttac
     1921 tggtcgatgt tcgtggcgag cttgaggtat tgcgaagcaa gggcgccaat attatttttg
     1981 aaggtgcgca aggtgcgtta ctagacatcg atcatggttc ttaccctttc gtaacatctt
     2041 caaacactgt ggcgggtgct gtaactacgg gggccggctt tgggccactt tatattgatg
     2101 aagttatagg tgttactaaa gcatattgta cgcgcgtggg ttcaggtccg tttccgacag
     2161 aattaagtga tgcaacaggg gaactacttc gatcacgcgg gcaagaatat ggtgcaacca
     2221 caggcagagc gcgtaggtgt ggttggtttg atgtttgttt aatgcgacat tcggcattta
     2281 tcaacagcat ttctcactgc gtaattacca agttggacgt tttagacgaa tgtgatgaaa
     2341 taaaaatctg tacacattac cgattaggag aaaaattgct tacacatcct cctgtcagca
     2401 gtgaagattt tggaaaatgt gagcctattt atgaaacatt gccaggttgg aaaacttcaa
     2461 ctttaggtgt aatcgattat cgtgatttgc cccaaaatgc tcagaagtat ctagaaagga
     2521 tagcattcct ggcaaaaata aaaatttcga tgatttctac gggtgctgag cgtgcgcata
     2581 atatcaaatt a
//
LOCUS       DASTIB010000210         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1417487, whole genome shotgun sequence.
ACCESSION   DASTIB010000210 DASTIB010000000
VERSION     DASTIB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417487"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFP93_03575"
     CDS             complement(<1..308)
                     /locus_tag="VFP93_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843720.1"
                     /translation="MAMRGPIQNILSAKARFNAVAVNPEQLGIRLDEIQSFIADFLYR
                     FKNDEKALLIHYKNNDKFKALRQDFIQKLNKLSIEYKNSHVDWYSSYNQPHMALEGKL
                     "
     gene            complement(408..1271)
                     /locus_tag="VFP93_03580"
     CDS             complement(408..1271)
                     /locus_tag="VFP93_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843721.1"
                     /translation="MLAFNAEVENQLIRKFSSRTVEQISAKMEKYQTSIQKFVAENGY
                     ISLAQFLVARNFPLLNPTDSGETLLHYAVQTPGNINMVRWVLEQNLSLSDKNELQWNV
                     FHYLACFCRDEIILDLIFEQNFSSKDLFAYDINDEQPLIIALKTDNHLFLNRALSKLK
                     SHIDAQESLYLWNLTKDQNNLMHLIVHYNAVHCADLCRKDPELQSMLNIPNSQGKLPL
                     QLPQAKEKMDAFLSLQGWKNERVFSLMWQATKELKKAGIANNLMSEYIDPIEKEQKEK
                     DAFLKGCRSSG"
     gene            complement(1402..3438)
                     /gene="uvrB"
                     /locus_tag="VFP93_03585"
     CDS             complement(1402..3438)
                     /gene="uvrB"
                     /locus_tag="VFP93_03585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET9843722.1"
                     /translation="MQKKFQLKAEFSPAGDQTQAIKGLSEGIKEGKKFQTLLGVTGSG
                     KTFTMAHVIQNIQKPTMILAPNKTLAAQLYGEMRNFFPNNSVQYFVSYYDYYQPEAYV
                     PSSDTYIEKDASINEHIEQMRLSATKSLLERKDTIIVGTVSAIYGLGDPNKFLSMMLH
                     ISRGDLVDQRRILLRLAELQYTRNDMELHRGTYRVRGDVIDVFPAESEKESIRISLFG
                     NEVESLHYFDPLTGELLYEMPRATIYPKTHYVTPRDQVLSAIENIKEELKIRLEELYQ
                     ANKLLEAQRLRERTMYDVEMLLELGYCNGIENYSRYLTGRKPNEPPPTLLDYLPKDSL
                     LMLDESHVLVPQLGGMYRGDRARKETLVEYGFRLPSAMDNRPLRFEEFETISPQTIYV
                     SATPADFEINRSERIVEQIVRPTGLVDPKIEIRPAHSQVEDILSEIHNRVKNQERILI
                     TTLTKRMAEDLTEYLAEHNVKVRYLHADIETVERVEIIRDLRLGVFDVLVGINLLREG
                     LDMPEVSLVAIFDADKEGFLRSTRSLIQTIGRAARNIQGTAILYADVVTNSMRNAIEV
                     TEQRRAKQLAHNEIHGIVPKTIKKEISDIMEGARWTKKRQQEFKNSSRQPLLPVMKEM
                     RELQYKALSPTQLNKYLKQWENQMFDYANNLQFEEAARVRDALQNFKLNLLSEQ"
     gene            3536..4717
                     /locus_tag="VFP93_03590"
     CDS             3536..4717
                     /locus_tag="VFP93_03590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9843723.1"
                     /translation="MAIRLSDRVGRIKPSATLAISQRAQNLKTQGKDIVDMGTGEPDF
                     DTPSHIKEAAIQAMRDGKTKYTAVDGISSLKEAILMKFANFNGLNFSADQVMVSSGGK
                     QCIYNLFQSTLNSGDEVIIPIPYWVSYPDIAILAQATPVFIETTMQTRFKLTPAQLDN
                     AITSKTKLLILNSPSNPTGIAYTENELKLLGEVLLKHPNVFVATDDMYEHILWTEKGF
                     CNILNTTPELYDRVVVLNGVSKAYAMTGWRIGYCAGPSHVIKAMKNLQSQSTSNPCSI
                     AQAAAEAAIRGPLDLVAMMVKSFKERHDYLVSALNNISGIKCASADGTFYVFPNVQQA
                     IEKIGIASDTEFADHLLSTVGLAVVPGSAFGAPGHLRLSVATSMDTLEKAVDRLNKAL
                     M"
BASE COUNT         1416 a          893 c          857 g         1569 t
ORIGIN      
        1 aaaagcttac cttctaaagc catatgaggt tggttatagc tggaatacca atcaacatgg
       61 gaatttttgt attcaatact taatttattt aatttttgaa taaaatcttg tcttaaggct
      121 ttaaatttat cattattttt ataatgaata aggagggctt tttcatcatt tttaaatcga
      181 taaagaaaat cggcaataaa gctttgaatt tcatctaatc gaattccaag ctgttcagga
      241 ttaacagcaa ctgcattaaa gcgtgccttt gcacttaaga tattttgaat gggaccacgc
      301 atagccataa tttttcccct aattgtctaa tttatttata taaacagctt ataaggttct
      361 aaaagtatgg ctcaacaaaa ttgcgactaa tggtttattt gagaggatta accagaagat
      421 ctacatcctt ttaaaaaagc atctttttct ttttgttcct tttctatagg atcgatgtac
      481 tctgacatca gattatttgc tattccggct ttcttcaatt ctttggttgc ctgccacatt
      541 aaagaaaaaa ccctttcatt tttccaacct tgcaaactta gaaaggcatc cattttttcc
      601 tttgcttggg gtaattgtaa tggtagtttc ccttgtgaat taggaatatt aagcatagat
      661 tgtagttctg gatctttgcg acacagatcc gcgcaatgaa ccgcattata atgcactatt
      721 aaatgcatta agttgttttg atctttagtt aaattccaaa gatataaact ttcctgcgca
      781 tcgatgtgag attttaattt tgacagggct ctatttaaaa aaagatgatt atctgtttta
      841 agtgctatga taagtggctg ttcgtcattg atatcatatg caaacaaatc ctttgaagaa
      901 aaattttgct cgaaaattaa gtctaaaatt atttcatcgc gacaaaagca cgctaaataa
      961 tgaaacacat tccattgaag ttcgttttta tccgatagag aaagattttg ttctaaaacc
     1021 catcgcacca tatttatatt tccaggagtc tggactgcat aatgtaatag tgtttcccca
     1081 ctatcggtgg gatttaacaa cgggaaattt cttgcaacta aaaattgcgc tagagaaata
     1141 tagccatttt ctgcaacaaa tttttgaatt gaggtttggt atttttccat ttttgcggaa
     1201 atttgttcga cagtacgaga agaaaacttt cgtattagct gattttcaac ttctgcgtta
     1261 aacgctagca tgtagcaccc tgggattaaa aacgactatt ttggcagtaa atatactaaa
     1321 attgtatcat caaaaagtca actgttattt atgcaactta ttaaagctct tcgggcaaaa
     1381 tttaccgtat aacaagtaat tttactgctc acttaataaa ttcaatttaa aattttgcaa
     1441 ggcatctcgc acccgtgcag cctcttcaaa ttgcaaatta tttgcgtaat caaacatttg
     1501 attttcccat tgcttcaagt atttattcaa ttgtgtagga cttaaagctt tatattgtaa
     1561 ctctctcatt tccttcatta ccggtaataa aggctggcga ctagaattct taaattcttg
     1621 ttgacgtttt ttcgtccaac gtgccccttc cataatgtca gaaatttctt ttttaattgt
     1681 ttttggcaca ataccatgaa tttcattgtg tgctaattgt tttgcgcgac gctgttcggt
     1741 aacttctatt gcattgcgca tcgaatttgt tacaacatcg gcatacaaaa ttgcggtacc
     1801 ctgaatattt cgtgcagctc gaccaatagt ttgaattaaa gagcgcgttg accgtaaaaa
     1861 accttcttta tccgcgtcaa aaatagcaac cagcgagact tcaggcatat ctaaaccctc
     1921 acgcagcaaa ttgattccca ccaatacatc aaaaacaccg agtcgcaaat cacggataat
     1981 ttccacgcgt tctacggttt caatatctgc atgtaaatag cgcactttta cgttatgctc
     2041 tgctaaatat tccgttaaat cttcagccat tcttttggtt aacgttgtaa ttaaaattcg
     2101 ctcttgattt tttacacgat tatgaatttc ggaaagaata tcttcaactt gagaatgcgc
     2161 gggacgtatc tcaatttttg gatccaccag tcctgtcggt cgaacaattt gttctacgat
     2221 acgttccgaa cgatttattt caaagtccgc aggcgttgca gatacataga ttgtttgtgg
     2281 agaaatagtt tcaaattctt caaatcgtaa aggtctatta tccatagcag aaggtaagcg
     2341 aaacccgtat tctacgaggg tttctttacg agctctatcc ccgcgataca tacctcctaa
     2401 ttgtggaact aatacgtggg attcatctag cattagtaat gaatctttag gtaaataatc
     2461 caataaggta ggcggtggtt cattcggctt tcttcctgtt aaatagcgcg aataattttc
     2521 aatcccgttg caatagccca attccaataa catttccaca tcgtacattg tgcgctcgcg
     2581 caaacgctgc gcttctaata atttattagc ttgatacaat tcttctaagc gaatctttaa
     2641 ctcttcttta atattttcta tagcgcttaa tacttgatcg cgcggagtta cataatgcgt
     2701 ttttggatat atggtggctc ttggcatttc atacaaaagc tcgcctgtca atggatcaaa
     2761 ataatgtaaa ctttctactt catttccaaa taaagaaatg cgtatcgatt ctttttccga
     2821 ctctgcagga aagacatcaa tcacatctcc acgtacacga taagtgcctc gatgtaattc
     2881 catatcattg cgagtatatt gcagttcagc taagcgtaat aaaattctgc gctgatcgac
     2941 caagtctcct cgtgaaatat gtaacatcat cgataaaaat ttattcggat cacctaaacc
     3001 gtaaattgca gagaccgtac cgacgataat ggtatctttg cgttcaagta atgatttagt
     3061 ggcgcttaag cgcatttgtt ctatatgttc gttgatagaa gcatctttct caatataagt
     3121 gtcagaggac ggcacatacg cttctggttg atagtaatca taataagaca caaaatattg
     3181 cacagaatta ttgggaaaaa aatttcgcat ctcaccataa agttgggcgg ctagggtttt
     3241 atttggcgct aaaatcatgg tgggtttttg aatattttgt ataacatgtg ccatagtaaa
     3301 ggttttaccg gagcctgtta cccccaataa ggtttggaat tttttgcctt ctttgattcc
     3361 ctcagataaa cctttaattg cttgtgtttg atctcctgca ggggaaaact cggcttttag
     3421 ttgaaatttt ttttgcatag taatttgcga tattagttac cataaattgt gaagctacac
     3481 tataatgggc aaaatgccca ttattacttt gtgccagttt aacataggag gtaacttggc
     3541 tatacgacta tctgaccggg ttggcagaat aaaaccatcg gcaacattag ccattagtca
     3601 acgtgctcaa aatttaaaaa cacaaggtaa agatattgtt gatatgggaa cgggtgagcc
     3661 cgatttcgac actccctcac atatcaaaga agccgccatt caggctatgc gcgatggcaa
     3721 gacaaaatat accgcggttg atggcattag tagtttaaaa gaagctattt taatgaaatt
     3781 cgccaatttt aatggtttga atttttctgc agatcaagta atggtgtcta gtggtggaaa
     3841 acaatgtatc tataacttgt tccaatccac gcttaattct ggcgatgaag taatcatacc
     3901 tattccttat tgggtttctt atcccgatat tgcgattttg gctcaggcaa ctccagtttt
     3961 tattgaaacc acgatgcaaa cgcgatttaa actaacgcct gctcaattag ataatgcgat
     4021 aacttcaaaa acaaaactgc ttattttaaa tagtccttca aatcctacgg gtattgccta
     4081 tacagaaaat gaattgaaat tattggggga agtattatta aagcacccca atgtttttgt
     4141 tgcgaccgat gatatgtatg agcatattct ttggacggaa aaaggttttt gtaacatttt
     4201 aaatactacg ccagaacttt atgatcgggt tgttgtttta aatggcgttt ccaaagctta
     4261 tgcgatgaca ggttggagaa ttggctattg tgccggtcct tcgcacgtaa taaaagctat
     4321 gaaaaatctt caatctcaaa gtacctctaa cccttgttct attgcgcaag cagctgctga
     4381 agctgctata cgcggtcctt tggatttggt tgcaatgatg gtcaaatctt ttaaagaacg
     4441 acacgattac cttgtcagtg ctttaaataa tatatcggga ataaagtgcg cttccgcaga
     4501 tggaacattt tacgtgtttc ctaatgtcca acaggcaata gaaaaaattg gcatcgcatc
     4561 tgacacggaa tttgccgatc atctgctgag cactgtaggg ctcgccgtag ttccaggctc
     4621 tgcctttggg gcccctggac atctgcgttt atccgttgca acgagtatgg acactttaga
     4681 gaaggcagtc gaccgattaa ataaagcgct gatgtaagct ttaccatggg agagg
//
LOCUS       DASTIB010000211         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1001075, whole genome shotgun sequence.
ACCESSION   DASTIB010000211 DASTIB010000000
VERSION     DASTIB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001075"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="lon"
                     /locus_tag="VFP93_03595"
     CDS             <1..1281
                     /gene="lon"
                     /locus_tag="VFP93_03595"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9843724.1"
                     /translation="GGVKDEAEIRGHRRTYIGSMPGKILQKLAKVEVRNPLFLLDEID
                     KMAQDFRGDPASALLEVLDPEQNSTFNDHYLEVDFDLSDVMFVATANSLNIPPPLLDR
                     TEVIRLAGYTEDEKLHIATKYLVPKQITNNGLQEDEIALPESSLLSIIRYYTREAGVR
                     NLEREISKICRKVVTRVSMDENKIKLTINPENLEEYLGVKKFRFGIAEEHDQVGQVTG
                     LAWTEVGGDILTIESVATEGKGKVTRTGSLGGVMQESIQAALTVVRSRAQRLGIMPNF
                     YEKKDIHVHVPEGATPKDGPSAGIAMCTALVSALTAIPVRADVAMTGEITLRGEVLPI
                     GGLKEKLLAARRAGIVHVIIPEENRKDLKEIPDNILEKLKIHCVKWIDEVFDLALSRP
                     PQPLSDDEIIKEESSGVKSKRKNKKQASDTLDKH"
     gene            1577..1894
                     /locus_tag="VFP93_03600"
     CDS             1577..1894
                     /locus_tag="VFP93_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9843725.1"
                     /translation="MSNAPGNKAALIEWMAQSSDISKTAATKALNAVIDGITQALREG
                     NSVAILGFGTFMVKTRAERTGRNPKTGEPIKIAAANIPSFKAGKALKDAVNSGSVVVE
                     EEV"
     gene            2008..2083
                     /locus_tag="VFP93_03605"
     tRNA            2008..2083
                     /locus_tag="VFP93_03605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2041..2043,aa:Val,seq:tac)
     gene            2101..2177
                     /locus_tag="VFP93_03610"
     tRNA            2101..2177
                     /locus_tag="VFP93_03610"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2135..2137,aa:Asp,seq:gtc)
     gene            2237..>3268
                     /locus_tag="VFP93_03615"
     CDS             2237..>3268
                     /locus_tag="VFP93_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET9843726.1"
                     /translation="MLQKIRDKSQGWFAWVVIGLIALTFILIGTGTYFGSEPNDVLAK
                     VNGQEITQADVNQYYERLLLQQGSNSSNFDAKQIKQYALEVLMQEAALKATAKEKGLR
                     VSKDQILQVIAQDNNFYDKGEFSNEKYHQLLAQNYFTPQQYQDLIEFQFLIAQLQHGL
                     MQTNFSLKNELNTLFKFLDQKRNFTYHIVNHKEFLNQVSVNDQEIKEYYDAHRDQFMT
                     EEQIKIQYLELNTKDLKKDISYTESDLEQFYQDKIDLYTEPQKIKVAHILVAFDAQDA
                     KAKDAAIAKINNIKTKLSQGESFAKVAQQYSDDKLSAKNGGELSWITQGEAALIADFE
                     KAAFAMKEVN"
BASE COUNT         1095 a          559 c          702 g          912 t
ORIGIN      
        1 gggggtgtta aagacgaggc tgaaattcga ggccaccgtc ggacttatat cggatctatg
       61 cctggaaaaa ttttgcagaa attggcaaaa gtggaagtta gaaatccatt atttttgtta
      121 gatgaaattg acaaaatggc gcaagatttt cgcggtgatc ctgcttcagc acttttagaa
      181 gtgttggatc ctgaacaaaa cagtactttc aatgatcact atttagaggt cgattttgat
      241 ttgtcagatg ttatgtttgt agcaacagca aattctttga atattccacc tccattatta
      301 gatagaacgg aagtcattag acttgcaggt tatacggaag atgaaaaatt acatattgca
      361 acgaagtatt tagtaccaaa gcaaattacc aacaacggtt tgcaagaaga tgaaattgca
      421 ctaccagaat cgagtttgtt aagtatcata cgatactata cccgtgaagc tggtgtgaga
      481 aacttagaaa gagaaatttc aaaaatttgt cgtaaagttg taacccgtgt ctccatggat
      541 gagaacaaaa taaaattaac cattaaccct gaaaatcttg aagaatattt aggcgttaag
      601 aagtttcgtt ttggcatcgc ggaagaacat gatcaagtag gtcaagtaac aggattggca
      661 tggacagaag taggtggaga tattttaact atcgaaagtg ttgcaacaga aggaaagggt
      721 aaagtaacgc gtacaggttc gcttggaggg gtaatgcagg aatccattca agctgcctta
      781 accgtcgtgc gcagtcgagc acaacgtttg ggaattatgc ccaattttta tgaaaagaaa
      841 gacatccacg tgcatgtgcc tgaaggtgca acacctaaag atggccctag cgcaggcatt
      901 gcaatgtgta cagcactcgt atctgcactt actgccatcc cagtgagagc ggacgttgca
      961 atgacaggag aaattacgtt acgtggtgaa gtattgccaa ttgggggatt aaaagaaaag
     1021 ttattagctg caagaagggc aggtatcgtt cacgttatta ttcccgagga aaatcggaag
     1081 gacttaaaag aaattccgga caatattttg gaaaagctaa aaatacattg tgtgaaatgg
     1141 attgatgagg tttttgattt agcattatct cgtccgccgc aacccttatc ggatgatgaa
     1201 atcatcaaag aagagagcag tggagttaaa tcaaagcgta aaaacaaaaa gcaagcgtct
     1261 gacacgttgg acaaacatta aaacttctct acttaatgta aataaagcca ggattttggc
     1321 tttatttaca tttctaaata aaaattttct tcatattttc atttttagtt aattcttacg
     1381 ggcaactatt ggagcgatag agataattct ttttagttag cccttgttcc ctaggtctgg
     1441 aagtgataat ttgccgttta ttgtgatgcc atgataccaa tcacaattta aataaataac
     1501 tatttttctt ggattccata tattggcatt ttggttatcg atgatatggc caagagttag
     1561 gcaaaagagg tgagatatga gtaacgcacc tggaaataaa gcggcattaa ttgaatggat
     1621 ggcgcaatct tcagacatct ccaaaacagc agcaactaag gctttaaatg ctgtaattga
     1681 tggtattacc caagctttaa gagaggggaa ttccgtggct attttaggtt tcggtacctt
     1741 catggtaaaa acaagggctg aaagaactgg acgcaatcca aagactggtg aacctattaa
     1801 aattgctgct gcaaatattc cttcatttaa ggctggtaaa gctttaaaag atgcggtaaa
     1861 ctccggctcc gttgttgtgg aagaagaagt ttagggatga agataggcag tagctctgcc
     1921 tggaaaaatg gacaaaatca agggttgaga taaaaaaatt tgtgttgact cgcaaaaatt
     1981 tttcactaaa atgctcatcc atgaatcggg tgcttagctc agctgggaga gcgtcgccct
     2041 tacaaggcga gggtcggggg ttcaagtccc tcagcaccca ccaaattttt gaccgtattt
     2101 ggagcggtag ttcagttggt tagaataccg gcctgtcacg ccgggggtcg cgggttcgag
     2161 tcccgtccgc tccgccaact cttaaggcgt gttgtattct tcgagtacac acgccttttt
     2221 tattcgggga gcagccatgt tgcaaaaaat tcgggataaa tcacaaggtt ggtttgcttg
     2281 ggttgtaata ggactaattg ctttaacgtt tatcttgatc ggtactggaa cttattttgg
     2341 ttccgagcca aacgatgtgt tggcaaaagt gaacggtcag gaaataacgc aagcagacgt
     2401 taatcaatac tacgagcgct tattattgca gcaaggttcg aattcctcta attttgacgc
     2461 taagcaaatt aagcaatatg ctttagaagt attgatgcaa gaggcggcct taaaggcaac
     2521 ggctaaagaa aagggcttga gggtatcaaa agatcaaatt cttcaggtaa ttgctcaaga
     2581 taataatttt tacgataagg gtgaattctc caacgaaaaa tatcatcaat tacttgctca
     2641 aaattatttt acacctcaac agtatcaaga tcttattgaa ttccaattcc taatcgcaca
     2701 attacaacac ggtttaatgc aaaccaattt ttcgttaaaa aatgaattaa acactttgtt
     2761 taaattctta gatcaaaaaa gaaattttac ttatcacatc gttaatcata aagaatttct
     2821 taatcaagta tctgtcaatg atcaagaaat caaagaatat tatgatgcgc accgcgatca
     2881 atttatgaca gaagagcaaa tcaaaattca ataccttgaa ttaaacacca aggatttaaa
     2941 aaaagatata tcctacacag aaagtgactt agagcaattt tatcaagata aaatcgatct
     3001 ctacaccgaa ccgcagaaaa ttaaagttgc acatatttta gttgcttttg atgcgcaaga
     3061 tgcaaaagca aaagatgcag ccattgctaa gatcaacaac attaaaacaa aattatcgca
     3121 aggtgagagc tttgcaaagg tagcacaaca atactctgac gataagctat cagcgaaaaa
     3181 tggcggtgag ttaagttgga tcacgcaagg tgaagctgct cttattgcag attttgaaaa
     3241 agctgcattc gcaatgaaag aagtaaat
//
LOCUS       DASTIB010000212        10025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_299868, whole genome shotgun sequence.
ACCESSION   DASTIB010000212 DASTIB010000000
VERSION     DASTIB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299868"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..2362
                     /gene="rnr"
                     /locus_tag="VFP93_03620"
     CDS             107..2362
                     /gene="rnr"
                     /locus_tag="VFP93_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET9843727.1"
                     /translation="MSKKKKINKYIKDPFGDREAKRYEKPIPSREFILEILLSNPQPI
                     PQKKLAKILALTDPDQQEALRRRLNAMVRECQIALMPKGYTALTMFKEQTGILKVEKD
                     GFGTVSGEETKVKLPPREIRGFYTGDKLRVRITRYDFEGQAFGSVIELIQATIVKLVG
                     RFTRENGHGYVVVLGKKYAQDIHIPQGKSKQAQDGEIVQVVVDRRASTLQVPVGHIEQ
                     VLGEPDREGIEIDVAILKYQVPYLWQKGVEHEAKKFGKSIPKREVSEREDLTHLPLVT
                     IDGEHAKDFDDAVFCETRKNGWRLYVAIADVSHYVLSESKLDLEASTRGNSVYFPGKV
                     IPMLPEHLSNGLCSLMPNVERLVLVCEMLITQSGSVTRSKFYPAVIRSRAQLTYTLVS
                     TLLKENQSVPKQYAELYPHLKALEQLYYALDGARRLRGAIDFNSVETQIVFDKKGKIA
                     GVVARERTIAHKIIEECMLAANVSAARFLKRHKIPTLYRVHLPPPEEKLNALRDFLAE
                     LGLGLRKRDFPTSKDYSELIHAVQTRDDVHIIETVMLRSMAQAEYNVTQEGHFGLAFP
                     AYLHFTSPIRRYPDLIVHRQIKAILAREKINRDAWHEKLSLLGSHCSMTERRADDATR
                     EVALSLKCQFMAERVGEIFQGRISAVTAFGLFVELEDFYVEGLIHVATLGDDYYFYEP
                     FKHRMIGERTRKIYSLGDKLEVQIMRVDVEDKKIDLEIVGAQKTNAQSAIRKRKGTKR
                     KRNTDKRKKRY"
     gene            2362..3105
                     /gene="rlmB"
                     /locus_tag="VFP93_03625"
     CDS             2362..3105
                     /gene="rlmB"
                     /locus_tag="VFP93_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET9843728.1"
                     /translation="MKTIMIGGIHAVKQALALAPTRVEKIFLDKKVPKQLKSLEDLAK
                     SLNILSQKCDRAVLDIKVEGAHHQGVVAQLKEIDPILEADALERLQGAFNPVIVLLDE
                     VQDPQNVGGILRSCAAFGANMLVVPERNSCKITASVRKTACGGDLIVPFLEVKNLARF
                     IRGLQEIGIWIVGTSIDPSSLKLTRCDFKGPIALVMGAEGSGIRQNTAKQCDFLVRLP
                     LSPNMESLNVSVATGICLYECWRQRQDNT"
     gene            3243..3599
                     /gene="rpsF"
                     /locus_tag="VFP93_03630"
     CDS             3243..3599
                     /gene="rpsF"
                     /locus_tag="VFP93_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9843729.1"
                     /translation="MRHYEIFLLVHPDQGEQLPAMIERYQGMITNAGGAIHRHEDWGR
                     RQLAYPIEKIHKAHYVLMNIECNNETLAQLKHAFKYNDAILRDLIIKRKQAITEPSAI
                     LKPDEDKDRAIDIDDI"
     gene            3609..3845
                     /gene="rpsR"
                     /locus_tag="VFP93_03635"
     CDS             3609..3845
                     /gene="rpsR"
                     /locus_tag="VFP93_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9843730.1"
                     /translation="MAKRFRGNKKKFCPFAEGKIPSSAIDKTNVRFLRKFITDTGKIV
                     PSRITGVSAKYQHAIVREIKHARFLALLPYCDRH"
     gene            3861..4316
                     /gene="rplI"
                     /locus_tag="VFP93_03640"
     CDS             3861..4316
                     /gene="rplI"
                     /locus_tag="VFP93_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9843731.1"
                     /translation="MEVILLENIKNLGRFGTKVKVASGFGRNFLIPQGKAVPATKENM
                     EKFEADRANLEKAAQTRLQKAQERAAEIEAAHITLHVRAGDEGKLYGSVGTLELARAF
                     KEQGIEISRQEILLPNGPLRELGDFEVLCQLHAEVKAKAKVSLIPEENK"
     gene            4475..5860
                     /gene="dnaB"
                     /locus_tag="VFP93_03645"
     CDS             4475..5860
                     /gene="dnaB"
                     /locus_tag="VFP93_03645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9843732.1"
                     /translation="MTQEQQVRQPPHSIEAEQAVLGGLMINNQAWDQIADRIQEHDFY
                     QNEHQVIFRAMQALTSANKPFDIVTIPTWLEEKQWLEKAGGWEYLKELAENTATAANV
                     VVYADIIRERSILRQLLRVGLEIQETASKPEGRTSSEILDEAEQKVFNIAEKRSRNDG
                     PENIKTIAARALTNIEIRKNNPDSLNGLPSGFKDLDKLTNGLQKGDLVIIAGRPSMGK
                     TILGINIAEHVAIKSKKAVLVFSMEMPSESLANRMLASLGRIDLSRLHNGKLKDDEWN
                     RLATGVQWLDQANMFIDESPALSPIEVRARARRLVRDGHTLGLIVIDYLQLMRCGWHA
                     ENRTAEISEISRSLKALAKELNVPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDA
                     DLIVFIYRDEVYNEESPDKGSAEILIAKHRNGPIGMVRLTFMGHYAKFENYLSSQAVI
                     MPQGVTTSVIE"
     gene            5862..6941
                     /gene="alr"
                     /locus_tag="VFP93_03650"
     CDS             5862..6941
                     /gene="alr"
                     /locus_tag="VFP93_03650"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9843733.1"
                     /translation="MSRNLIATISRHALKHNLQTVKTFAPHARIIAMVKANAYGHGLQ
                     EVCKTLQNADAFGVASLEEAILIREIGLKNPVVLMEGFFKESEIASIERFCFTCVVHS
                     DYQIKILEKYPGTYRIPFWIKINTGMNRLGLPFEKVNDAFDRLISKKNLHFLGFMGHF
                     PQADDISNPITLTQYQKFADYLKKYPGFRSLANSATIMQWPACHHDWVRPGLMLYGAS
                     PFSMRSAQELNLLPAMELRSEIIAIQQVKKGEQVGYGGTWVGKDSSHTIAIVAVGYGD
                     GYPWHAQNGTPILVQEKEALLCGRVSMDMIAIDITNIPAAIGESVTLWGGKLPIERIA
                     QHAKTVPWELFCRLTERVKFTWVDQ"
     gene            7058..7495
                     /locus_tag="VFP93_03655"
     CDS             7058..7495
                     /locus_tag="VFP93_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016212305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9843734.1"
                     /translation="MALKKKMTTKGRKPAKKTLAKVARNTNTVKKATTKVVNLPTAAI
                     TSKQTKAQIYNEIVEITGLSKPEVKKVTGALRNIIERHVKAKGSGEVTLPDLGIKVRR
                     IQKKATKARMGRNPFTGDEIRIAAKPPRKSVKATALKLLKALA"
     gene            complement(7544..8017)
                     /locus_tag="VFP93_03660"
     CDS             complement(7544..8017)
                     /locus_tag="VFP93_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9843735.1"
                     /translation="MTLSLSSSAFENFQEIPKMYTCQGDDISPPLAWQNIPEKAKSLV
                     LIVDDPDAPDPNDPKMTWVHWILYNLPPDIHALKEGITQEELPFGTLEGLNDWKKTGY
                     GGPCPPIGQHRYFHKLYALDIQLPDLKHPTKSELEKAMEGHILAKAEYLGTYIKQ"
     gene            8226..9005
                     /locus_tag="VFP93_03665"
     CDS             8226..9005
                     /locus_tag="VFP93_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET9843736.1"
                     /translation="MNVKKFGLGLVFTMLSTLASPLHAELSPTDKEEINKMIHQYLTQ
                     KPEVLKEALQALQTQEWESMESATQKAILEHSDILFQSKTSPTIAPAKPTVTVVEFFD
                     YQCKYCKKMRQDLNQFFEKNTDIRMVYKELPVLGETSIVASKAALAAHKQNKYMDFHN
                     RLMQEESITEQKIYEIAKELKMNLEQLKKDMESKEIMAELRSNIQLARDLGLRGTPAL
                     IVAPYPTKVQMKSMHVPGAITAEQLNEVVTRIKTDNNTQIN"
     gene            complement(9002..9550)
                     /gene="ppa"
                     /locus_tag="VFP93_03670"
     CDS             complement(9002..9550)
                     /gene="ppa"
                     /locus_tag="VFP93_03670"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET9843737.1"
                     /translation="MGLMRVPAGREVPEDVNVIIEISAHSEPIKYEVDKNTGALFVDR
                     FMGTPMRYPCDYGYIPQTLCQDGDPVDVLIITPFPVSRGCVLRCRPVGMLLMEDEEGP
                     DAKILAVPIEKLTPLYNHVKKPEDLPELLLKQIIYFFEHYKDLEPGKWVKLNGFTGAK
                     EAKVEILHSIERYKNLPDELAF"
BASE COUNT         3238 a         1845 c         2065 g         2877 t
ORIGIN      
        1 ccccacaagt acctgaaact tgcgtgtcta ccagtttcac cacctcggct tggaaaactt
       61 tgcaaaaatc taacctttat aatcctgaaa gtcaaccata aaaattatgt caaaaaagaa
      121 aaaaattaat aaatatataa aagatccatt tggggaccgt gaagctaaac gttatgaaaa
      181 acccattccg agtcgcgaat ttattctaga aatcttgctc agcaatccac agccgattcc
      241 tcaaaagaaa ttagccaaaa ttctagcgtt aactgaccca gatcaacaag aagcattacg
      301 acgtcgctta aatgctatgg tacgtgaatg ccaaatcgca cttatgccta aaggttatac
      361 tgccctgact atgttcaaag aacaaacggg tattttaaaa gttgaaaaag acggttttgg
      421 cactgtttct ggcgaagaga caaaagtaaa actaccgcct cgtgaaattc gagggtttta
      481 taccggggac aaattaagag tacgtattac gcggtatgat ttcgaaggcc aagcatttgg
      541 gagtgttatc gagcttattc aagcaaccat agtgaaatta gtgggacgtt ttacccgcga
      601 gaatggacat ggttatgttg tggtgttagg aaaaaagtat gcgcaggata tccatattcc
      661 ccaaggtaaa agcaaacagg cacaagatgg ggaaattgta caagtggttg tcgataggcg
      721 tgcttcgact ttacaagtac cggttggtca tattgaacaa gttctcggag aaccggatcg
      781 cgagggcatt gagattgacg ttgctatact aaaatatcaa gtgccttatc tttggcaaaa
      841 aggtgttgaa catgaagcaa aaaaatttgg caaatccatc ccgaaacgag aagtttcgga
      901 acgtgaagat cttacccact tacccttagt caccatagac ggtgagcatg ctaaagattt
      961 tgatgatgcg gtattttgcg aaacccgaaa aaacggctgg cgcttgtatg tagcaattgc
     1021 ggatgtaagc cattatgtat tatccgagtc gaaattagac ttggaagctt ctacccgcgg
     1081 aaattcagtt tattttcctg gaaaagtcat tcccatgctg ccagaacatc tatccaatgg
     1141 attgtgttct ttaatgccca atgttgaacg attagtgctt gtttgtgaaa tgttaattac
     1201 ccaatcaggc agcgttacac gatcaaaatt ttatcctgca gttattcgat ctcgtgcgca
     1261 actgacttat acgttagtga gcaccttatt aaaagaaaac cagtcagttc caaaacaata
     1321 tgcagaatta tatccacatc taaaagcttt agagcagttg tattatgcat tggatggcgc
     1381 tcgacgtttg cgaggtgcaa ttgattttaa tagtgttgaa acgcaaattg ttttcgataa
     1441 gaaaggtaaa atcgcaggtg ttgttgcgcg tgaaagaaca attgcccaca aaattatcga
     1501 agaatgtatg ttggccgcca atgtaagcgc tgctcgattt ttaaaacgcc ataaaatacc
     1561 aacactatac cgagtacatc tcccaccgcc tgaagaaaaa ttgaatgctt tacgcgattt
     1621 tttggctgag ttaggtttgg gtttgcgaaa aagagatttt ccaacatcca aagattattc
     1681 tgaattaatt cacgctgttc aaacaagaga tgacgtgcat attattgaaa ccgttatgtt
     1741 acggtcgatg gcacaggctg aatataatgt aacgcaagaa ggacactttg gtttagcgtt
     1801 tccagcctat ttacatttta cgtctccgat ccgacgttac ccagatttaa ttgtacatcg
     1861 tcaaattaaa gcaatcttgg ctagagagaa aataaacaga gatgcatggc acgaaaaact
     1921 ttctctgctg ggttcgcatt gttctatgac ggaaagaagg gcagacgatg ccacccgaga
     1981 agttgcctta tcactcaaat gtcaattcat ggcagaacgt gtaggagaga tttttcaagg
     2041 tcgcatttct gctgtcaccg catttggatt gtttgttgaa ttagaagatt tctatgtaga
     2101 aggtttgatt catgttgcaa cactcggcga tgattattat ttttatgaac cttttaaaca
     2161 ccgtatgata ggggagcgta cccgaaaaat ttattcctta ggggataaac tagaagtgca
     2221 aattatgcgt gtggacgtgg aagataaaaa aatagatttg gaaatcgtag gtgcccaaaa
     2281 aactaacgcc caatctgcta tccgaaaacg taagggtacg aaacgtaaga gaaacacaga
     2341 taagcgtaaa aagagatatt aatgaaaacg ataatgattg gtggcattca tgctgtcaaa
     2401 caagcgctag cgttggcacc tacccgcgtt gaaaaaattt ttttagataa aaaagtgcca
     2461 aagcaattaa agtctcttga agatcttgca aaatcattaa atattttatc gcaaaaatgc
     2521 gatcgcgcgg tattagacat taaggttgaa ggagcgcacc accaaggggt tgtagcgcaa
     2581 ttaaaagaaa tagatcccat acttgaggcc gatgcacttg aacgattgca gggggctttc
     2641 aatccagtaa tagtattact ggatgaagtg caagatccgc aaaatgtagg cggtattttg
     2701 cgtagttgcg ccgcatttgg tgcaaatatg ttagtagtcc cggagaggaa ttcttgcaaa
     2761 atcactgcga gcgtacggaa aactgcgtgc gggggggatt tgatagtacc ttttcttgag
     2821 gtaaaaaatc ttgcacgttt tataagggga ctccaagaaa ttggcatttg gattgtaggt
     2881 acaagtatcg atccatcctc tctaaaatta acgcgctgcg attttaaggg tcctatagcg
     2941 ttagttatgg gggctgaagg ctctggtata cgtcaaaata cggcaaaaca atgtgatttt
     3001 ttggtgcgat taccactatc ccccaacatg gaaagtttaa atgtatctgt ggcgacgggc
     3061 atttgtttat acgagtgttg gcgtcaacga caagacaata cgtaaggttt tagagttgta
     3121 agtgtagctt gcttgcctta taattctcag ccttggtatt tttaaccaag gcagttaatc
     3181 aattaacttc tccttgctct cactttcggg tagagctgat aaaagccaaa aggagcttat
     3241 caatgcgtca ttatgaaata tttttgttgg tacaccctga tcaaggtgaa caattacctg
     3301 caatgatcga acgatatcag ggtatgatta ccaacgcagg cggggcaata caccgtcatg
     3361 aagattgggg tcgcagacaa ttggcctatc ctatcgaaaa aattcacaaa gcccattatg
     3421 ttctaatgaa tatagaatgc aataatgaaa cgcttgcgca attgaaacac gcatttaaat
     3481 acaatgatgc gattttgaga gatttaataa ttaagcgcaa acaagcgatt actgaacctt
     3541 cggctatttt aaaaccagac gaagacaaag atcgcgctat tgatatagat gatatttaag
     3601 ggggcaatat ggcaaaacgt tttcgtggta ataaaaagaa attctgtccc ttcgcagaag
     3661 gaaaaattcc aagcagcgct atcgataaaa ccaatgtgcg tttcttaaga aaatttatta
     3721 ccgatacagg taaaattgtg ccaagtcgta tcacgggtgt atctgcaaaa taccaacatg
     3781 ctattgtccg tgaaattaag catgcaagat ttttagcact attaccttat tgcgataggc
     3841 actaagtaag gggattttag atggaagtta ttttactcga aaatattaaa aaccttggac
     3901 gctttggtac caaagtaaaa gtggctagcg gatttggacg taatttttta attccgcaag
     3961 gcaaggctgt tcctgctaca aaagaaaaca tggaaaagtt tgaagcagat cgtgctaact
     4021 tagaaaaagc ggcacaaact cgcttacaaa aagcgcagga aagagctgcc gaaattgaag
     4081 ccgcacatat aactttacac gttcgtgctg gggatgaagg aaagctttat ggttccgtag
     4141 gtacacttga acttgcgcgt gcctttaaag aacaaggtat tgaaatatca aggcaagaaa
     4201 tactattacc taatggtccc ctacgtgaat taggcgattt tgaagttctt tgccaattac
     4261 atgcggaagt aaaggcaaaa gctaaagtta gtttaattcc cgaagaaaat aaataatcta
     4321 aactctacca agtgtttact tggtagagct catattccat ttcttctatg atcttgcacc
     4381 ctcggtttag ttagtgacag aatgatgcca agtgattcct taacttgtta tgtagggtat
     4441 tttggtgacc gattcatctt ataaaaattt tggcatgacc caagagcaac aagtgcgcca
     4501 acccccacat tccatagagg ctgagcaagc agtgttgggt ggattaatga taaataatca
     4561 agcttgggat caaattgcag ataggatcca agagcatgat ttctaccaaa acgagcatca
     4621 agtcatattt agggcgatgc aagctttaac ttctgctaat aaaccttttg atattgtaac
     4681 tatacctacg tggcttgaag aaaaacaatg gttagaaaaa gccggcgggt gggagtattt
     4741 aaaagaactt gcagaaaata ccgcaactgc cgctaatgtt gttgtgtatg ccgatatcat
     4801 tcgcgaacga tccatattac gtcaattatt gcgtgtaggt ttggaaattc aagaaactgc
     4861 ttcgaaacca gaaggccgca ccagctcaga aattttagat gaagcagaac aaaaagtttt
     4921 taatatagcg gaaaagcgtt cgcgtaacga cggtccagag aatatcaaaa ctatagcggc
     4981 ccgagcactt acaaatattg aaattcgtaa aaacaatcca gattccctaa atggtttacc
     5041 ttccgggttc aaagatctcg ataaactaac caatggttta caaaaaggtg acttagtaat
     5101 aattgcaggt cgaccttcta tgggtaaaac tattttgggt ataaatattg ctgagcatgt
     5161 cgcaatcaaa tcgaaaaaag ccgtattagt attcagcatg gaaatgcctt ccgaaagctt
     5221 agccaatcgt atgctggctt cgttgggccg aattgattta agtcgtttac ataatggtaa
     5281 attaaaagat gacgaatgga atcgattagc gacaggtgtg caatggttgg atcaagccaa
     5341 tatgtttatc gacgagtcac ccgctttaag tcctattgaa gtgcgcgctc gggcgcgtag
     5401 attagtccga gatggacata ctttaggtct tattgtaatt gattatctgc aattaatgcg
     5461 ctgtggttgg catgcggaaa ataggaccgc tgaaatatca gaaatttccc gatcgctaaa
     5521 agctctcgct aaagaactta acgttcctgt tattgcttta tcccagctca atagaagtct
     5581 tgaacaacgt cccaataagc gacccgtgat gtccgatttg cgcgagtctg gcgcaattga
     5641 gcaagatgcc gatttgattg tgtttatcta tcgagatgaa gtttataatg aagaaagtcc
     5701 agataaaggt tctgcagaaa tccttattgc aaaacatcgt aatggtccca ttggcatggt
     5761 gcgcctaaca tttatgggac attatgcaaa atttgaaaat tatctgagtt cgcaagctgt
     5821 tataatgcct caaggtgtaa ctacttctgt gattgaataa aatgagccgt aatttaattg
     5881 ctactatttc tcgtcatgct ttaaaacaca atctacaaac ggtaaaaaca tttgcgcctc
     5941 atgcccgcat aattgcgatg gttaaggcaa atgcctatgg ccatggattg caagaagttt
     6001 gtaaaacctt acaaaacgcc gatgcttttg gagttgcgag tttagaagaa gcaattttga
     6061 ttcgtgaaat tggcttaaaa aatccggtag tgctcatgga aggttttttt aaagaatccg
     6121 aaattgcgag tatagaaaga ttttgtttca cttgtgtcgt ccattcggat tatcaaataa
     6181 aaattttaga gaagtatcca ggtacttacc gtattccatt ctggatcaaa atcaatacgg
     6241 ggatgaatag acttggttta ccttttgaaa aagtaaacga tgcgtttgat aggttaattt
     6301 ctaaaaaaaa tcttcatttt ttaggattta tgggtcattt tccacaggct gatgatattt
     6361 cgaatcccat aacacttacc caatatcaaa aatttgcaga ttatttaaaa aaatatccgg
     6421 ggtttagatc ccttgcgaat tcagccacaa ttatgcagtg gcctgcttgc caccatgatt
     6481 gggttcgtcc tggactcatg ctgtatgggg cctctccatt ttctatgcgt tccgcgcaag
     6541 aattgaatct tttgcctgct atggagttac ggtcagagat cattgccatt caacaggtaa
     6601 aaaagggaga gcaagtgggt tatggtggca cttgggtggg taaagattct tcccatacaa
     6661 ttgccattgt cgcggttggg tatggcgacg gttatccctg gcacgcgcaa aatggaacgc
     6721 ctattttagt acaagaaaaa gaagcgctac tttgtggtcg agtttctatg gacatgattg
     6781 ccatagatat cacaaatatt cctgccgcaa ttggtgaatc ggtaacgctt tggggtggaa
     6841 aattacccat cgaacgtata gctcagcatg ccaaaaccgt tccttgggag cttttttgtc
     6901 gtttaaccga gcgggttaaa tttacctggg ttgatcaata aacacttttc tccaaaaatt
     6961 cactatttgt actattttat taatttctct agcattaacc cctaattttc atttatacta
     7021 cgccgcgtca aaatatagtt attatttgga gattagaatg gcattaaaaa agaaaatgac
     7081 aacgaaaggc cgtaaacctg ctaaaaaaac tttagcaaaa gttgctcgta atacaaatac
     7141 cgtgaaaaag gctactacaa aagttgtcaa tttgcctacc gctgccatca ctagcaagca
     7201 aacgaaagcg caaatttata atgaaattgt agaaatcaca ggcttaagca aacctgaagt
     7261 aaagaaggtt accggtgctt taagaaacat tatagagcga cacgttaaag ctaagggctc
     7321 tggcgaagtt actttgcctg atttagggat aaaagtacgt cgtatacaaa agaaagcaac
     7381 taaagcgcgt atgggtcgta atcctttcac cggcgatgaa ataagaattg ctgctaaacc
     7441 accccgcaaa tccgtaaaag ctaccgcttt aaaactttta aaagcgttgg cttaatttaa
     7501 aaaaatccca gcactcgctg ggatttttgt aaagattacg tatttattgt tttatgtatg
     7561 tgcctagata ttcggctttc gctaaaatat gaccttccat agctttttct aattcacttt
     7621 tggttgggtg ttttaagtct ggcaattgaa tgtccagtgc ataaagctta tgaaaataac
     7681 ggtgttggcc aatgggcgga cacggaccac cataaccggt ttttttccaa tcgttcaatc
     7741 cttctaacgt cccgaaaggt aactcctcct gggtaatccc ttcctttaaa gcatgtatat
     7801 cgggaggcaa attgtaaaga atccaatgta cccatgtcat ttttggatcg tttgggtcgg
     7861 gcgcatcggg atcatccaca ataagcacga ggctttttgc tttctcagga atattttgcc
     7921 atgcaagagg aggtgaaatg tcatcgcctt gacaggtata catttttgga atttcctgaa
     7981 aattctcaaa agcagaagag gaaagtgata gggtcatacg tgctccatgt gcattggact
     8041 gaataaaaat taaggtgaaa aaatgcttac aaaaaaatta taaattctca cgtctaagcg
     8101 atctcgacac cattaggaaa ttccttattc tggataaaaa tcacgtcttt gtctatctgc
     8161 ctttagacta aacttttatc aatatttaat tttttgatac caaactaatt tacagaggtt
     8221 actgtatgaa tgtaaaaaaa tttggattag gcttggtgtt tactatgtta agtacgttag
     8281 cctctccttt gcatgccgaa ttatcaccta ccgataaaga agaaattaat aaaatgattc
     8341 atcaatattt aacacaaaaa ccagaagttt taaaagaagc tttgcaagcg ctacaaaccc
     8401 aggaatggga atctatggaa tccgccacgc aaaaagcaat tttggagcac agtgacatac
     8461 tctttcaaag taaaacgagc cctacaattg cccccgcgaa gcctacggta actgtggttg
     8521 aatttttcga ttatcaatgt aaatattgca aaaaaatgcg acaagatctg aatcaatttt
     8581 ttgaaaaaaa taccgatatt cgcatggttt acaaagagtt acccgtctta ggggaaacgt
     8641 ctatcgtagc gtcaaaagca gcactcgcag cgcataaaca aaataaatat atggactttc
     8701 ataatcgatt aatgcaagaa gagtctatta ccgagcaaaa aatttatgaa attgccaaag
     8761 aactcaaaat gaatttggag caattaaaga aagacatgga atctaaagaa attatggccg
     8821 aattgcgcag taatatccaa ttggcacgtg atttgggatt acgaggaaca cctgcactca
     8881 ttgttgcccc ttatcccacg aaagtgcaaa tgaaatctat gcatgtacca ggagctatca
     8941 ccgcagaaca gctaaacgag gttgttacac gaataaaaac ggataacaac acgcaaatta
     9001 attaaaatgc tagctcatcg ggtaagtttt tatagcgttc gatggaatgt aaaatttcaa
     9061 ctttcgcttc ttttgcaccc gtaaaaccat tcagttttac ccattttccg ggttctaaat
     9121 ctttatagtg ttcaaaaaaa taaataattt gttttaaaag taattcaggt aaatcttcag
     9181 gtttttttac atggttataa aggggagtaa gtttttcaat aggtacagcc aatattttag
     9241 catcaggtcc ttcttcgtct tccattaata acatgcctac cggcctgcaa cgtaaaacac
     9301 agcctcggct aacaggaaaa ggcgttatga taagtacatc aaccggatca ccgtcttggc
     9361 atagagtttg cggaatataa ccataatcac atggatatcg cattggggta cccataaaac
     9421 gatcgacaaa taaagcgccc gtatttttgt ctacttcata tttaattggt tcactgtgtg
     9481 cggaaatttc aatgatgaca ttaacatcct caggaacttc acggcccgct ggtactcgca
     9541 taagacccat aatttaccta cataacatgt tcgtggtttc tcatattata acccttgaaa
     9601 atgagcctgc actaacaatt tactttggaa atttaacttt ctgacggata aaagtgatga
     9661 ttttttctta cacctattaa aaaaactcag tacactgtcg ttctgaaagt tagtgttaaa
     9721 atcccaacag aaatgggatt agttccaagt tcatttttgt taagagcttg gggttaagct
     9781 gttgtaaatt cttgctccat cctggaacgt taaaaaaatt catagttgct acgtgaaatg
     9841 aataacgatg gaatcctttt tttattaatt taaaggatct tgcaattgaa tcgatgcgac
     9901 tatgttggcc gattaaagtt agtttgatac atatattaat aagaagattc gtaaaagtct
     9961 aaaatagaat agtctgtaaa acttgcatgc aagacatggt ttaacacttg cttagctagc
    10021 agtga
//
LOCUS       DASTIB010000213         9392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_644007, whole genome shotgun sequence.
ACCESSION   DASTIB010000213 DASTIB010000000
VERSION     DASTIB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644007"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..770
                     /locus_tag="VFP93_03675"
     CDS             168..770
                     /locus_tag="VFP93_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843738.1"
                     /translation="MRYAINVGLKIPNTLISNDPKEIINFIDSNNKNNANTVYKIFKP
                     VIWEEENKKYNFYTTTIERKALEGDYFLTPGIFQNYVKKAFEVRVTFFGSKYIAIKIN
                     NSETIDWRKIKFSDIRVEQFVLPTEIFFKCQLLMKKLGIIFGCFDFVVTRDNEFYFLE
                     VNEMGQFLWIDELLPELNLLDTFCNFMIDSQVKPLQPQIA"
     gene            879..1799
                     /locus_tag="VFP93_03680"
     CDS             879..1799
                     /locus_tag="VFP93_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843739.1"
                     /translation="MELDENLYAEFAGVEVISADKNVVNKIKKNISLKIGQKLRLAET
                     EQYKNTCEQLVTKYFDIPKLKCDFIYFSDGFLYLTINIVPKRTTRISQKNNYIIYKNS
                     LNVPNKLMFLLHKLEERQIFLINHGIPLTEKNFNNTLDFDDYILHSIAYQLRSLVPQY
                     NNTLLMILRDSNNPFERENAAQLLSWANEPKNLPFLLANHLFYDSALGVRNNLLRSYT
                     ITMKNKVFDNYKSELLVVSCNVAMLPSHFDRNKGLVLLKTILNSNPHMIYAIRPECSR
                     TIKLISEASVMDNVGGVAREILALSNAIKE"
     gene            2009..2182
                     /locus_tag="VFP93_03685"
     CDS             2009..2182
                     /locus_tag="VFP93_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843740.1"
                     /translation="MKKQVKTESTAKTKRAFARLLSNELPMEILSDISGGKWSCKGTV
                     NEKHVAVDCGNYS"
     gene            complement(2478..3242)
                     /locus_tag="VFP93_03690"
     CDS             complement(2478..3242)
                     /locus_tag="VFP93_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9843741.1"
                     /translation="MEIKNLLAVVTGAASGVGRACAETLGALGATVILIDINKEKIDE
                     IAKTIKGIACAIDITDPQAVEDAFAQIVANQTLPLRILINCAGIAPASRIFGKEGCKP
                     LDFFSKVINVNLIGTYNVLRVASFYMSKQEVLETTERGVIINTASIAAFEGQIGQTAY
                     SASKAAIVGLTLPAARELAQFGIRVMTIAPGLLDTPMLSTLPQDVRESLEKQTPFPKR
                     FGKPEEFAALAQSIIENPMLNGEVIRLDGGLRMQPS"
     gene            3397..4560
                     /locus_tag="VFP93_03695"
     CDS             3397..4560
                     /locus_tag="VFP93_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HET9843742.1"
                     /translation="MIPTVLFQYNETASLIRESVQNFAKHEIAPIAHKIDSDNQFPNH
                     LWKKMGDLGLLGITVDEQYGGTNLDYLAHVIAMEEISRASGSVGLSYGAHSNLCVNQI
                     HRNGNEEQKQNYLPKLISGEHVGALAMSEVHAGSDVMHLSLNVSEKSDHFVLNGTKMW
                     ITNGPDADIIVLYGRTLKNAGSKGITAFIIEKNFKGFHSAQKLDKLGMRGSNTCELVF
                     EDCEIPKENILGQLHEGSHVLMSGLNYERLILAGGPLGIMQSCLDVVLPYVRERKQFG
                     KAIGEFQLIQAKLADMYTRLQSARAFTYAVAKTADENHALTNKDAAAVILLAAESATQ
                     SALDAIQCLGGNGYINEYPTGRLLRDAKLYEIGAGTSEIRRLVIGKELFSASE"
     gene            4567..5754
                     /locus_tag="VFP93_03700"
     CDS             4567..5754
                     /locus_tag="VFP93_03700"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9843743.1"
                     /translation="MKHYPHIVIVGHARTPIGDLLGHFSNTPAYMLGAKAIQGAITKS
                     TLNPNDLDEVVMGCVLPAGQGQAPARQAAIHAGIPVHVGATTINKMCGSGMRSLMMCY
                     DSILAGSTAIAAAGGMENMSLAPFLLPKMRAGVRMGHSQIYDHMFLDGLEDAYEKSQL
                     MGIFAEQCVAHYQFTREEQDDFAIASLQRAQNAIKNGLFKQEIVSIEVQTKKETLIID
                     TDQLPGRAQPDKIKTLKPAFKKEGSITAANASSISDGASAHILMSIHEAEKRGIKPLA
                     RIVGHATHSQEPAWFTTAPVGAIQKVLEKAQWEIKDVDLFEINEAFACVTLAAMRELD
                     LPHDKVNVHGGACALGHPIGASGARITSTLIEALKHYGLKRGIAALCIGGGEATALAI
                     EIL"
     gene            5751..7358
                     /locus_tag="VFP93_03705"
     CDS             5751..7358
                     /locus_tag="VFP93_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET9843744.1"
                     /translation="MTLLKTQIDIHSQEFVERFELMQMHVKALHDLIEQLKQGGGHDA
                     QQKHVKKGKLFVRDRINHLCDPGAPFLELSQLAGYKLYEDEVAMGGIVAGIGRIERQE
                     CMIIANDATVKGGTYYPITVKKHLRAQEIAQQNHLPCLYLVDSGGANLPYQDEVFPDK
                     DDFGRIFYNQAIMSSLKIPQIAVVLGSCTAGGAYVPAMADQSIIVKNQGTIFLAGPPL
                     VKAATGEIVTAEELGGADVHCRISGVADYYAQDDQHALKIARECIRHLARKKNVPIAS
                     QESKPPLYDPNEIYGLIPIDLKTPMDVKEIIARIVDGSKFHEFKALYGATLVCGFAHI
                     HGYAVGIIANNGILFNESAQKGTHFIELCCQRNIPLIFLQNIAGFMVGKRYEAQGIAK
                     DGAKMVTAVACAKVPKFTVIIGGSFGAGNYGMCGRAYEPRFLWMWPNARISVMGGEQA
                     ASVLSQVKSTNQKKAQQAWDEQAQKDFTQQIIQQYEKQGHPYYSSARIWDDGVIDPAQ
                     TRNILGLSLSVAYHAPVESTQFGVFRI"
     gene            7355..8152
                     /locus_tag="VFP93_03710"
     CDS             7355..8152
                     /locus_tag="VFP93_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9843745.1"
                     /translation="MTNEQTDSILCRIESDVAHVILNRPDKSNALDIPMILQLNEVLA
                     NLRNMHTLRVLWLKGAGKNFCAGADLQWMQESIHFDKKQNYDESLQLAQLMYNLYTLP
                     FPSLVTVQGACFGGGVGLVASCKIALATPSTKFCFSEAKLGLIPAVISPYILQALGVR
                     ATEALFLTSEMFNGTRAHELGLIQKIVEDANLESEGKLLSQILTKQSPLALKAIRDLV
                     DRVHDKKIAQDLIEETSRNIAEIRVSSEAQEGVKAFLDKRIPKWVQP"
     gene            8149..>9392
                     /locus_tag="VFP93_03715"
     CDS             8149..>9392
                     /locus_tag="VFP93_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9843746.1"
                     /translation="MIRKILIANRGEIACRIIQTCKKLGIHSVAVYSDIDRNARHVQL
                     ADEAYYLGSNPAQQSYLNIEKILSVAKRAKAQAIHPGYGFLSEKASFAKACELAGLIF
                     IGPKADVIEAMGNKDRAKTLMENAKVPIMPGYHGSDQTLNRLLEEAKSLLPLMIKARA
                     GGGGKGMRIVHHVDDILDSLESAKRESAKNFHDEALVLEKYLPQARHIEVQVFGDNFG
                     NHVHLFERDCSLQRRHQKIIEEAPALNMGQEIKEALYQTALNACKAIQYTNAGTFEFL
                     FTPFDNKFYFLEMNTRLQVEHPVTEMITGLDLVEWQILIANNEPLPLKQEEIACFGHA
                     IEARIYAEDASNHFFPATGEVKALILPNDREVRLELGLLKNDKIDIHYDPILAKIVAY
                     EKDRTKAIQKLSRTLQQTEIIGV"
BASE COUNT         3099 a         1669 c         1865 g         2759 t
ORIGIN      
        1 gcttgactat ccctaaaata cccaaaaacg ttaacaagtt tgataagaaa tttattgaat
       61 cggaaatacg aacttatatc cgatccttat ggttaagcat aggtgaaaaa tcaaaataga
      121 ttaatccatt ttcttcctac gatgcatcga acagcaaagt aattcaattg cgatatgcta
      181 taaatgttgg attaaagatt ccaaatactt taatatcaaa tgatcctaaa gaaattatta
      241 attttattga ttcaaataat aaaaataatg caaataccgt ctataaaatt ttcaaacctg
      301 ttatctggga agaagagaat aaaaaatata atttttatac aacaacaata gaaagaaaag
      361 cattagaagg tgattatttt ttgacgccag gtatttttca gaattatgta aagaaggcat
      421 ttgaagttcg tgtaacattt ttcggcagta agtatatcgc aataaaaatt aataatagcg
      481 aaactataga ttggagaaaa attaaatttt ccgatataag agttgaacag tttgtcttgc
      541 caactgaaat attttttaaa tgtcaactat taatgaaaaa gttaggaata atttttggtt
      601 gttttgattt tgtagttact cgagacaacg aattttattt cttggaagta aatgaaatgg
      661 gccaatttct ctggatcgat gaactattgc cagaattaaa tttactggat actttttgta
      721 attttatgat agatagtcaa gtaaaaccgc tacaaccgca aatcgcttag acatgcgtat
      781 aatacgttat aaaattattt aaaattaagc tatgcatgaa aataaatata attcgattat
      841 tgttattaac aagtttgatt tgtagaactg tttattgtat ggaacttgat gaaaatttat
      901 atgcagaatt tgccggagta gaagtaatta gcgcagacaa aaatgttgta aataaaatta
      961 aaaaaaatat ttcattaaaa ataggtcaaa aattacgatt agcagagaca gaacaatata
     1021 aaaatacctg tgaacaatta gttacaaagt attttgacat tcctaagtta aaatgcgatt
     1081 ttatttactt cagcgatggt ttcttatatt tgacaataaa tattgttcct aaacgtacca
     1141 caagaatttc acagaagaac aattacatta tctataaaaa ttctttgaat gtaccgaata
     1201 aattaatgtt tttgcttcac aaacttgaag aacgccaaat ttttctgata aatcatggca
     1261 ttccacttac cgaaaaaaat tttaataata ctcttgactt tgatgactat atactgcata
     1321 gcatagcata tcaacttcgt tctttagtac cacaatataa taataccctt ttaatgattt
     1381 taagagattc gaacaatccc tttgaaagag aaaatgctgc gcaattactt tcctgggcta
     1441 atgaacctaa aaatttacca tttttattag cgaatcattt attttacgat tctgctctag
     1501 gagtgcgaaa taatttattg cgatcttata ctatcaccat gaaaaataag gtttttgata
     1561 actataaaag tgaattacta gtagtatcat gcaatgtagc aatgttgcca tctcactttg
     1621 atcgcaataa aggacttgta ctattaaaaa caattttaaa tagtaatcca cacatgattt
     1681 atgccataag acctgaatgt agtcgaacaa taaaattaat aagcgaagca agcgtaatgg
     1741 ataatgttgg cggagttgca agagaaatac ttgctttatc aaatgcgatt aaggaatgaa
     1801 gcattgataa ataatttaaa taaatactcg ccaatccatg gatgtaaatt gattaagtct
     1861 gttttacagg gattcacaat actaccacat aactatgcgc gaacgcattc gatttttttg
     1921 acactatttg tttaacatga aataattatt ttcgataact ttccaagttg tcgaaactga
     1981 aaaatataat tacgctagga gctaaacaat gaaaaaacaa gttaaaactg aatcaactgc
     2041 taaaacaaaa agagcttttg caagattact tagcaatgaa ttgccaatgg aaatattaag
     2101 tgatatttct ggaggtaaat ggagttgcaa aggaacagtc aatgaaaagc atgtagcagt
     2161 ggattgcggt aattattcgt gattcgtgat agcaaattta ttcttgctaa atacatatat
     2221 ggtaactaga aatgaataga tcatcctaat tacgcatcct tgtttcttac aaggatatct
     2281 aagaaacatt taaatataaa taataaacaa gacatagttt ttcaatattt ggaaataaaa
     2341 taaatttatt atcaaacagc ataatggctc aaattttatg aatcagcgag tattaactat
     2401 tacctatagt aatatatagc aacgaaatta atgtctaacc ctattttgtt tgagtaaata
     2461 ttttaaagcc agtgcaatta agaaggctgc attcttaatc caccgtctaa gcggatcact
     2521 tcgccattga gcatgggatt ttcgattatc gattgtgcca gcgctgcaaa ttcttctggc
     2581 tttccaaaac gttttggaaa aggagtttgt ttttctaaag attcacgaac gtcttgtggt
     2641 aaagtactga gcatgggagt atcaagtaaa ccaggcgcta tggtcataac acgaataccg
     2701 aattgtgcta attcccttgc tgcaggtaag gttaagccaa cgattgcagc tttagatgca
     2761 ctataagcag tttgccctat ttgcccttca aacgctgcaa ttgaggctgt attgattata
     2821 acacctcttt ctgttgtttc tagcacctct tgcttggaca tataaaaaga agctacgcgt
     2881 aatacattgt aggttccaat taaatttaca ttaattacct tagaaaaaaa atcaagaggc
     2941 ttacatccct cttttccaaa tatacgtgaa gcaggtgcta tacctgcaca attaattaaa
     3001 atccgtaatg gcagtgtttg attcgccaca atttgcgcaa atgcatcttc aaccgcttgt
     3061 ggatccgtta tatcgatcgc acacgctatg cccttaatgg tttttgcaat ttcatcaatc
     3121 ttctctttgt ttatatcaat cagtatcact gttgcaccaa gggcgcctaa tgtttcagca
     3181 caagctctgc ccactccact tgcagcgccg gtaactacgg ctagaagatt tttaatttcc
     3241 ataaatttct cgcaatttat tgcatactta agggtactaa gttaactcta catctacttg
     3301 agctttaagc aaaacaaagt tagagtgaat ttgatcccgc attttgttca agttattgag
     3361 cttgataaag gaagatatca caccagggag aatactatga tcccaactgt gctttttcaa
     3421 tacaatgaaa ctgctagtct catccgcgaa agcgtgcaaa attttgcaaa gcatgaaata
     3481 gcacccattg cgcataaaat cgattcggat aaccaatttc caaaccattt atggaaaaaa
     3541 atgggtgatt taggcttact cggcatcacc gtagatgaac aatatggcgg tactaactta
     3601 gattatttgg cgcatgtcat tgcgatggaa gaaataagtc gcgcttctgg ctcggttggc
     3661 ttaagttatg gtgcgcattc caatttatgc gtaaatcaaa tacaccgtaa tggtaacgaa
     3721 gagcaaaaac aaaattatct acccaaactt atttccggtg aacatgtggg tgcacttgct
     3781 atgtctgagg tacatgcagg ttctgatgtg atgcacttat cgcttaatgt aagtgaaaaa
     3841 tccgatcatt ttgtattaaa tggcacaaaa atgtggatca cgaatggacc ggatgcagac
     3901 atcatcgttt tatatggtag aactctaaaa aatgcgggct caaaaggtat tacggccttt
     3961 atcattgaga aaaattttaa aggttttcat agtgcacaaa aactcgataa gttgggcatg
     4021 cgcggttcaa acacctgcga actcgtgttt gaggactgtg aaatccctaa agaaaatatt
     4081 ttaggccaac ttcatgaagg atctcatgtt ttaatgagtg gattaaacta tgaacgttta
     4141 attttagcag gtggcccact tggaataatg caatcttgct tagatgttgt tttaccttac
     4201 gttcgggaac gcaaacaatt tggcaaagcc attggtgagt ttcaattgat acaggccaaa
     4261 ttagccgata tgtatacacg attgcaatcg gcacgcgcat ttacctatgc agttgcaaaa
     4321 accgcagatg aaaaccatgc attaaccaat aaagacgctg cagcggtaat attgttggct
     4381 gcggaatcgg caacccaaag tgctttggat gccattcaat gtttaggtgg taatggttat
     4441 atcaatgaat atcctacagg taggttatta cgggatgcaa agctgtatga gataggcgct
     4501 ggcacctcag aaatacgccg cttggttatt ggaaaggaat tattttctgc aagtgaatga
     4561 ggaattatga agcattatcc gcacattgta attgtaggac acgcaagaac acctatcggt
     4621 gatttgttag gacactttag caatacaccc gcctacatgt tgggagccaa agcaatacaa
     4681 ggcgcaatta caaaaagcac gcttaatcca aatgatttag atgaggtggt catgggttgc
     4741 gtattacccg cgggtcaagg gcaagcaccc gcacgccaag cagctattca tgcgggtatt
     4801 cctgttcatg tgggtgctac tacgataaac aaaatgtgcg gatcaggcat gcgatctctt
     4861 atgatgtgtt atgacagtat tttagcgggc tccactgcca tagctgctgc aggaggtatg
     4921 gaaaacatga gccttgcgcc ttttttactg ccaaaaatgc gcgcaggtgt acgtatgggg
     4981 cattcgcaaa tttatgatca tatgttttta gatggtttag aagatgcata tgaaaaaagt
     5041 caattaatgg gaatttttgc agagcaatgc gtagcacatt atcagtttac gcgagaagaa
     5101 caagacgatt ttgccatagc ttcattgcag cgtgcccaaa atgcgataaa aaatggtctt
     5161 tttaaacaag agattgtatc tattgaagta caaacaaaaa aggaaacatt aatcatagat
     5221 accgatcaat taccaggacg tgcccaacca gacaagataa aaacgttaaa gcctgctttt
     5281 aaaaaagagg ggtccattac tgctgcaaat gcaagttcca tttccgatgg tgctagcgct
     5341 catattctca tgtcgattca cgaagcggaa aaacgcggta taaaaccact agcgcgtatt
     5401 gttggacatg caacccactc gcaggagcct gcatggttta ccacggcacc tgttggagcc
     5461 attcaaaagg ttttagaaaa agcgcagtgg gaaattaaag atgttgatct ttttgaaatt
     5521 aatgaggcct ttgcttgtgt gacacttgcc gctatgcgtg agttagattt gccgcatgat
     5581 aaagtcaatg ttcatggcgg cgcttgcgca ttagggcatc ctattggggc atcaggtgcg
     5641 cgtattacat ccacactcat agaggcatta aagcactatg gtttaaaacg cggtattgct
     5701 gccttgtgta taggtggagg agaagccact gctttagcga ttgagatcct atgacattat
     5761 taaaaacaca aatagatatt catagtcaag aatttgttga acggtttgag ctcatgcaaa
     5821 tgcatgtaaa agctttgcat gatttgattg aacagcttaa gcaaggcggt ggtcatgatg
     5881 cccagcagaa gcatgtaaaa aaagggaaat tattcgtacg tgatcgcatt aaccatttgt
     5941 gtgatccagg agcgcctttt ttagagttgt cgcaattagc aggctataaa ctttatgaag
     6001 atgaagtagc catgggaggg attgttgcag gaattgggcg cattgaacgg caagagtgca
     6061 tgattattgc caatgacgct actgtaaaag gcgggacgta ttaccctatc accgttaaaa
     6121 aacatttacg cgcacaagaa atcgcccaac agaatcattt accctgcctc tatttagtcg
     6181 attccggtgg cgcaaatttg ccctatcaag atgaagtttt tcccgataaa gatgattttg
     6241 gcagaatttt ttataaccaa gccatcatgt ccagtttaaa aataccacaa atcgccgtgg
     6301 tgctaggatc gtgtactgcc ggtggtgctt atgtaccagc tatggcggat caatcgatca
     6361 ttgtaaaaaa tcaaggcacc atttttttag caggcccccc ccttgtgaaa gccgccaccg
     6421 gcgaaattgt aacggctgaa gaattaggtg gtgctgatgt gcactgtaga atatcaggcg
     6481 ttgcagacta ttatgcgcaa gacgatcagc atgccttaaa aattgcacgc gaatgtatac
     6541 ggcatctagc acgcaagaaa aatgttccca ttgcatccca ggaatcaaaa cccccgcttt
     6601 atgatccgaa tgaaatatat gggcttattc ccattgattt aaaaacacct atggatgtta
     6661 aagaaattat tgcccgcatc gtagatggat caaaatttca tgaatttaaa gcattatatg
     6721 gtgctacgct ggtgtgtggt tttgcacata ttcatggtta tgcagtagga attatcgcca
     6781 ataatggaat tctctttaat gaatctgcgc aaaaaggcac acattttatc gagctttgtt
     6841 gtcaacgcaa tattccgtta atatttttgc aaaatattgc tggctttatg gttggtaaac
     6901 gttacgaagc gcaaggaata gcaaaggatg gagcaaaaat ggtaacggcg gttgcctgtg
     6961 ctaaagttcc caaattcacc gttattatcg gcggtagctt tggtgctggg aattatggaa
     7021 tgtgcggcag agcttatgaa ccacgttttc tttggatgtg gcccaatgcc cggatatcgg
     7081 ttatgggtgg agagcaagct gcaagcgtgc tgagccaggt taaatctact aaccaaaaaa
     7141 aagcacaaca agcatgggat gaacaagcgc aaaaagactt tacgcaacaa attattcaac
     7201 aatatgaaaa acaaggtcac ccctattatt cgagtgcaag aatttgggac gatggtgtta
     7261 ttgatcctgc acaaacgcgc aatattttag ggctcagttt atccgtcgct taccatgctc
     7321 ctgtcgaatc tacccagttt ggagttttta gaatatgacg aacgaacaaa cagattctat
     7381 tttatgtcgc attgaatctg acgtggcaca tgtcattctg aatagaccgg ataaatccaa
     7441 tgccttagat atacctatga ttcttcagtt aaacgaagta ttagctaatc tgcgcaacat
     7501 gcacacgctt cgagtgcttt ggctgaaagg tgcaggtaaa aatttttgcg caggtgcaga
     7561 tttacagtgg atgcaagaaa gtattcattt tgataaaaag caaaattatg acgaatctct
     7621 acaactagcg caactgatgt acaacttata tactctaccc ttcccatcgt tagtgacggt
     7681 acaaggtgct tgttttggcg gcggtgttgg attagtggct agctgtaaaa tagcacttgc
     7741 cacaccatca accaaatttt gtttttctga agcaaagctc ggtttaatcc ccgcagtaat
     7801 tagtccctat atccttcaag ctttaggcgt gagagcgaca gaagctttat ttttaacatc
     7861 ggaaatgttt aatggcacgc gtgcgcatga acttggttta attcaaaaaa tagtagagga
     7921 tgcgaattta gaatccgaag gtaagcttct atcgcaaatt ttaacaaaac aaagtccttt
     7981 agccttgaaa gctattcgtg atctcgtgga ccgtgtgcat gataaaaaaa ttgcccagga
     8041 tctcatcgag gaaacttccc gtaacatcgc agagattcgc gtttctagtg aagcgcaaga
     8101 aggtgtaaaa gcctttttag acaaacgcat tcctaaatgg gtacaaccat gattcggaaa
     8161 atattaattg ctaatcgcgg cgaaattgcg tgtcgtatta ttcaaacttg caaaaaatta
     8221 ggcatacata gtgtcgctgt ttattccgac attgatagaa atgcgcgcca tgtccaatta
     8281 gcagacgaag catattattt aggtagcaac ccggcacaac aaagttatct caatattgaa
     8341 aaaattttaa gcgttgcaaa gcgcgctaaa gcgcaagcga ttcatccggg ctatggtttt
     8401 ttatcggaaa aagcatcttt cgcaaaagcc tgtgaactag cgggtttaat ttttattgga
     8461 cctaaagcag atgttattga agcgatggga aataaagatc gtgcaaaaac gctcatggaa
     8521 aatgcgaaag ttcccattat gcctggatat cacggcagcg atcaaacctt gaacaggctc
     8581 ttagaagaag cgaaaagttt actaccactc atgataaaag cgcgtgccgg cggtggtggt
     8641 aaaggaatgc gtattgtaca ccatgttgat gatattttag attcgctaga atctgccaag
     8701 cgcgaaagtg ccaaaaattt tcatgatgaa gctttggttt tggaaaaata tttaccacag
     8761 gcacgccaca ttgaagtaca agtgtttggc gacaattttg gaaaccatgt acaccttttt
     8821 gaacgcgatt gttccttaca aagacgacat caaaaaatta tagaagaagc tccagcctta
     8881 aatatgggtc aagaaataaa agaagctttg taccaaaccg cattaaatgc ttgtaaagcg
     8941 atccaatata cgaatgcagg tactttcgaa tttctgttca cgccatttga caataagttt
     9001 tattttttgg aaatgaacac acgcttgcaa gttgagcatc cggttaccga aatgattacc
     9061 ggccttgatt tagttgaatg gcaaatattg attgctaata atgaaccgtt gccattaaaa
     9121 caagaagaga ttgcgtgttt tggacatgca atcgaagcga gaatctatgc ggaagatgca
     9181 tctaatcatt tttttcctgc cacaggggaa gtgaaagctt taatattgcc caacgatcgc
     9241 gaagtacgat tagaattagg cttgctaaaa aatgataaaa tagatattca ttatgatccc
     9301 attcttgcaa agattgttgc ctatgagaaa gatagaacta aggctattca aaaattgtca
     9361 cgcaccttgc aacaaacaga aataattggc gt
//
LOCUS       DASTIB010000214         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1300570, whole genome shotgun sequence.
ACCESSION   DASTIB010000214 DASTIB010000000
VERSION     DASTIB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300570"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /gene="lolA"
                     /locus_tag="VFP93_03720"
     CDS             <1..608
                     /gene="lolA"
                     /locus_tag="VFP93_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HET9843747.1"
                     /translation="WVGIAFSAQSYPSQDLMKRLEEIQSYQAQFKQVIKDDKGKQIST
                     TEGDLQVARPGRFRWKSQTPDQVLVVADGKFIWTYDIELEQVVKNNQNTSLGQSPAAL
                     LAGEVVNLEKDYAVELAQPADCHRSVDACYKLVAKDQNNAFKNIKMGFSGKNLITIEM
                     QDALDQNILTEFKQVQVNQTMNNTIFQFKPPAGVDVIQGSN"
     gene            609..1889
                     /locus_tag="VFP93_03725"
     CDS             609..1889
                     /locus_tag="VFP93_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET9843748.1"
                     /translation="MQPLAIQLRPKTLEDIYGQEHLTAPGKPLNLVIEKKKLHSMILW
                     GPPGTGKTTLAQVLAQQVNIPFVALSAVMAGVKDIRAVVQKAQKDNTTSIVLFIDEIH
                     RFNKAQQDALLPYVEQGTIYLIGATTQNPSFEINQALLSRTRVYVLKPLSDDALKKVL
                     ERALQSESLGDANIQLSEGVDETLLEMASGDARRLLNLLEIIVQWIEAHSVESIITHE
                     IIAQVSDKKMAHFDKQGEHFYDLISALHKSVRGSEPNAALYWLARMLVGGVDPSYLSR
                     RIVRMASEDIGNADPCAMHLVLSAAETMERLGSPEGELALAQAVVYLALAPKSNAVYV
                     AFNAAMQTAESTSHFAVPLHLRNAPTHLMKKLDYGKGYQYDHNYPNAVAATQTYFPEK
                     LKTTSFYHPTSFGFEIQLKEKMQWLEELRKNAQE"
     gene            1892..3175
                     /gene="serS"
                     /locus_tag="VFP93_03730"
     CDS             1892..3175
                     /gene="serS"
                     /locus_tag="VFP93_03730"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9843749.1"
                     /translation="MLDPKKLRNNFEEIAQRLQLRGKTVDLNLFNLMEESRKKLQIET
                     QELQSERNKRSKEIGVLKSKGEDVSSFVKEMESLRDSLNDKEQALNALLGEQENFFLQ
                     LPNIPHASVPEGHNENANVEVRRWGNIPEFDFPVHDHIYLGELHQGIDFQTAALLSGS
                     RFVVLKGAIAKLHRALAQWMLDTHCNNGYQEVNVPFLVEEQSLFNAGQLPKFYDDLFH
                     LNGERKLSLIPTAEVPLANLLRDQIIDEKILPLKFVSHTPCFRSEAGSYGKDTKGMIR
                     QHQFEKVELFHFVHPTKSYEALEQITQDAETILQKLNLPYRVVSLCTGDLGFCASKTY
                     DLEVWLPGQNKYREISSCSNTEDFQARRLKARFRSGDGKPELLHTLNGSGLAIGRTLI
                     AVLENYQLPNGDIKVPDVLIHYMNGLHTLKAQNPE"
     gene            complement(3371..>3470)
                     /locus_tag="VFP93_03735"
     CDS             complement(3371..>3470)
                     /locus_tag="VFP93_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HET9843750.1"
                     /translation="TNYILATVSLYVAIYNLFLSLLQLLGALSGRE"
BASE COUNT         1161 a          634 c          702 g          973 t
ORIGIN      
        1 cttgggttgg gatcgctttt tctgcgcaaa gttatccttc tcaagactta atgaaacgtc
       61 ttgaagagat acaaagttac caagcacaat ttaagcaggt cataaaagac gataaaggta
      121 agcagatttc cacaaccgaa ggagatttac aggtggcacg acctgggcgt tttcgctgga
      181 aatcgcaaac acccgatcaa gtgctcgtcg tagcggatgg taaattcatc tggacatacg
      241 atattgaatt agagcaagtt gttaaaaaca accaaaatac ttctttaggg caatctcctg
      301 cggccttgtt agcgggcgaa gttgttaatt tagagaaaga ctatgcagta gaactcgcac
      361 aacctgcgga ttgccatagg tccgtcgatg cctgttacaa acttgtagca aaagatcaaa
      421 ataatgcttt taaaaatata aaaatgggtt tttcaggaaa aaatcttatt accattgaaa
      481 tgcaagatgc tttagatcaa aacattttaa ccgagtttaa acaagtacaa gtcaatcaaa
      541 ccatgaataa tacaatattt cagtttaaac caccggctgg cgtagatgtg attcaaggca
      601 gcaactaaat gcaaccattg gcgatccagc taagacccaa aaccttagaa gacatttacg
      661 gtcaagagca tttgacggct cctggtaagc ccttaaattt agttattgaa aaaaagaaat
      721 tacattccat gattttatgg gggcccccag gtacggggaa aacaacatta gcccaagttt
      781 tggcgcaaca agtcaacata ccatttgttg cgttatctgc agttatggca ggggttaaag
      841 acatacgtgc tgtagttcaa aaggcgcaga aagataacac cacctccatt gtcttattta
      901 tcgatgaaat tcatcgtttt aataaagcgc aacaagatgc gcttctacct tatgttgaac
      961 aaggtactat ttatttaata ggtgctacga cccaaaatcc aagtttcgaa attaatcaag
     1021 cattattatc tcgtacgcgt gtgtatgtat taaagcctct ctcagacgat gctttaaaaa
     1081 aagttttaga aagggcgctt cagagtgaga gtttgggtga cgcaaatatt caattaagcg
     1141 aaggtgttga tgaaacactt ttggaaatgg cgagtggaga tgcacgtcgg ttattgaatt
     1201 tactagaaat catagttcaa tggattgaag cgcatagtgt ggaatctata atcacccacg
     1261 aaattatcgc tcaagtatca gataaaaaaa tggcgcattt tgataaacaa ggagaacatt
     1321 tttatgattt aatatccgca cttcataaat ctgttcgtgg ttcagaaccc aatgccgctt
     1381 tatattggtt agcaagaatg ttagtcggtg gtgttgaccc gagttattta tcaagacgta
     1441 ttgtacgtat ggcctctgag gatataggca atgcggatcc ttgcgcaatg catttagtgt
     1501 tgagtgcagc tgaaaccatg gagcgcttag gctcacctga aggtgaattg gcattagcgc
     1561 aagcggtggt gtatttggca ctcgcaccaa aaagcaatgc cgtatatgtg gcttttaacg
     1621 cagcgatgca aaccgcggaa agcaccagtc attttgccgt tcctttacat ttaagaaacg
     1681 ccccaacgca tttaatgaaa aaactcgatt atggcaaggg atatcaatac gatcataatt
     1741 atcccaacgc cgttgctgct acccaaacgt atttccccga aaaattaaaa acgacttctt
     1801 tttaccatcc aacatccttt ggatttgaaa tacaattaaa agaaaagatg cagtggttag
     1861 aagaactccg caaaaacgca caggaataag tatgcttgat ccaaaaaaat tacgaaataa
     1921 ttttgaagaa attgcgcaac gcttacaatt acgcggcaaa accgttgact taaatttatt
     1981 taatcttatg gaagaatcgc gaaaaaaatt gcaaatagaa acgcaagaat tacaatcgga
     2041 acgtaataag cgttctaaag aaatcggtgt tttaaaaagc aagggtgaag atgttagttc
     2101 tttcgttaaa gaaatggaaa gtctgcgtga ttctttaaac gataaagagc aagcgttaaa
     2161 tgccttatta ggtgagcaag aaaatttttt tttacaatta ccgaatatac cgcacgcttc
     2221 ggtgccagaa ggtcataacg aaaacgccaa tgtcgaagta cgacgttggg gcaatattcc
     2281 tgaattcgat ttcccagttc acgatcacat ctatttaggc gagcttcatc aaggaatcga
     2341 ttttcaaaca gcggctttgc tttcgggatc ccgctttgtg gtattaaaag gggcgattgc
     2401 aaaattgcat cgagcgctag cgcaatggat gctagatacc cactgtaata acggatatca
     2461 agaagtaaac gtgccttttt tggttgaaga acaatctttg tttaatgcag gccaactacc
     2521 taaattttat gatgatttat ttcatctgaa tggtgagcgc aagctatcgc ttataccaac
     2581 tgctgaagtt cctttagcta atttacttcg tgatcaaatc attgatgaaa aaatccttcc
     2641 cttaaaattc gtaagtcaca caccttgttt tagaagtgaa gcagggtcct atggaaaaga
     2701 tacaaaaggc atgattcggc agcaccaatt tgaaaaagtt gagctttttc attttgtgca
     2761 cccaactaaa tcttatgagg cacttgaaca gataacacaa gatgcagaaa ctatattaca
     2821 aaagcttaac ttaccttatc gtgtagtaag tttatgtact ggcgatttag gcttttgcgc
     2881 aagtaaaacc tacgatttag aagtatggtt gcctggccag aataagtatc gagaaatatc
     2941 ttcctgttcc aacaccgagg attttcaagc acgtcgtcta aaagcgagat ttcgttctgg
     3001 cgatggtaaa ccagagctgt tacatacatt aaacgggtct gggttagcga ttgggcgaac
     3061 tttaattgcg gttctagaaa attatcaatt acctaatggt gatattaaag tgcctgatgt
     3121 gttaattcac tacatgaatg gattgcacac tttaaaagcg caaaatcctg aataaaaagc
     3181 tagattgatt tagaaggtaa ttatgtttaa agtccacttt agattaaata acaataacag
     3241 cacaaagtgc gctccgtacc cactacccca atggtgggaa taataagatg tcttgatgtt
     3301 taagatatct ataaaaaaga agtaaaataa aaaagccgct tttagcggct taatttgttg
     3361 ataaatacta ttattctctt ccgctaagtg caccgagcaa ttgaagcaat gataagaata
     3421 aattgtaaat agcaacatag agagacacag ttgctaaaat ataattcgtt
//
LOCUS       DASTIB010000215         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_948403, whole genome shotgun sequence.
ACCESSION   DASTIB010000215 DASTIB010000000
VERSION     DASTIB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948403"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="dnaA"
                     /locus_tag="VFP93_03740"
     CDS             complement(<1..867)
                     /gene="dnaA"
                     /locus_tag="VFP93_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9843751.1"
                     /translation="MDMSLWQNCLQRLKEEVSQSVFNQWLSPLQAETSPMGLTLWAPN
                     QYIFEGLTQDYLSRITQILSELNQGKTPRVVVGIGEKSLSKEDRAEVSEPTEVIQNVK
                     PVTTVQHQSNLNPSFVFDNFVVGKSNQLAEAAAKQVATNPGGAYNPLFLYGGVGLGKT
                     HLMHAIGNQILQQKVNGKVVYLHSERFVADMVKALQSNSINEFKRYYRSVDALLIDDI
                     QFFAGKERSQEEFFHTFNALLEGEQQIILTSDRYPKEIHGIEERLKSRFGSGLTVAVE
                     PPELETRVAILMS"
     gene            2012..2167
                     /gene="rpmH"
                     /locus_tag="VFP93_03745"
     CDS             2012..2167
                     /gene="rpmH"
                     /locus_tag="VFP93_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9843752.1"
                     /translation="MKRTLQRKSKLTRARTHGFRLRSRTKNGRKVLANRRRKGRKLLA
                     PTGGTTK"
     gene            2139..2549
                     /gene="rnpA"
                     /locus_tag="VFP93_03750"
     CDS             2139..2549
                     /gene="rnpA"
                     /locus_tag="VFP93_03750"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9843753.1"
                     /translation="MLQLVVPQNKQLGGLTIKLGFPRYRRLLKPDEFKRVFGKAKKVA
                     SGPFIILACPNQREYPRLGVVLSKKKIPKAVQRNRIRRLIRECFRKQTALGGVDFVVL
                     PKPGIDVLSNAQLEKGLLREWQQYTTWSSNRSLP"
     gene            2504..2749
                     /gene="yidD"
                     /locus_tag="VFP93_03755"
     CDS             2504..2749
                     /gene="yidD"
                     /locus_tag="VFP93_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9843754.1"
                     /translation="MATIHNVVKQSLITLIRGYQWCISPLIGPACRFYPTCSEYAKTA
                     ISDYGAIKGSWLIFKRLCRCHPLHPGGYDPVPRGTIE"
     gene            2746..>3226
                     /gene="yidC"
                     /locus_tag="VFP93_03760"
     CDS             2746..>3226
                     /gene="yidC"
                     /locus_tag="VFP93_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9843755.1"
                     /translation="MKDAINARVILLLTLVFLGMTLYGRWQEEHAPVSVNEPKQQSTT
                     PTTHEPDVPTDIPFSDAPTQTTAAPVETVQVDRLISVENNVLKLKIDKKGGDIVYAEL
                     KDYPKVKGKPEQGFVLLDKSKERFYIAQTGLIAKSGPDSRTLGRAEYTSQFSHYDISS
                     "
BASE COUNT         1001 a          619 c          606 g         1000 t
ORIGIN      
        1 gctcattaat atggccactc tggtttctaa ttccggaggt tcaacagcaa ctgttagacc
       61 agaaccaaat cgtgacttta accgctcctc aattccgtgg atctcttttg gataacgatc
      121 ggaggttaaa ataatttgtt gctcaccttc tagaagtgca ttaaaagtat gaaagaattc
      181 ttcttgagaa cgctctttac ccgcaaaaaa ctgaatatcg tcaataagga gcgcatctac
      241 agaacgataa tatcgtttga attcattgat tgaattgctt tgcaatgctt tcaccatgtc
      301 tgctacaaag cgttctgagt gcaaataaac tacttttcca ttcacttttt gctgaagaat
      361 ttgattgcca atagcatgca ttaaatgggt tttgcctaag ccaaccccgc catacaaaaa
      421 caagggatta taggcccccc ctgggtttgt cgcgacttgt ttcgctgcag cttctgccag
      481 ttgatttgac ttgccaacaa caaagttatc aaacacaaat gatggattta aattactttg
      541 atgttgaaca gtagtaacag gttttacatt ttggatgact tctgttggtt cgcttacctc
      601 tgctcgatct tctttggata atgatttttc cccaatgcca actacaactc gaggagtttt
      661 accttggttt aattcactta agatctgggt aattcgactc aaataatctt gggttaaacc
      721 ttcaaagatg tattgatttg gggcccataa cgttaagccc ataggtgacg tttcggcctg
      781 taacgggctt aaccattgat tgaaaacaga ttgggaaacc tcttctttca acctttgcaa
      841 acaattctgc cacaaggaca tatccagatc cacgcgcttc taaccccctc aataaagcta
      901 ggaaactagg cagtctttta agactgagtt ttggcttaag tttagaatta ataaaatttg
      961 ttatgacttt tgcttattat ttttttcttc taagcataaa atgtaaggtg catagttaaa
     1021 aaatcattca aaagccttcc tagtttatac gcagtcgtca aagttatcca cactgtattt
     1081 tctaaccaga aattaattca ttcaaattta ttacaatggt cattgtggat tattctgtta
     1141 ataagcagaa gttttagggg ttttttgatg tgctctttgt ggaaaaagat ttttttccac
     1201 actatatact agacaagacc aggcgttata cacaccattg cttgtatggc aaatgtttga
     1261 aaagcctaaa aaataacacc atatcctcga aatttcgcac ccttattatc ataaaaattt
     1321 ttttttttga tcgttattat ttttataaca accgatattt atccacagat cgagcacaaa
     1381 aacctgtgcg taactaggga gaaagtcttt aaaaaccctg tgtaccctca aagcacaaat
     1441 atttataagt gtttatccac cacttgttcc gtactaaaaa ctatgcccac ccacacagaa
     1501 tccatggagg taagtacctg atatttaaac gttaagatgg gttattaacc ataaatctgt
     1561 tcctaatcat tataaatatt ttcttaatag aaaatgataa ttgttattgg cgaagcagaa
     1621 cttaaaagtt tggaagtcga gcatttttta tacttcgagt atcttgtcca ccaacaatct
     1681 tgaagtttgt gtggataaat catgaaaaaa tgaacaatta cttcgaggcg ttcgattatt
     1741 ttttgaaaaa gcgcctttta tccccaggtt ttacaacgtt tatcggaaag ttttgcgcca
     1801 actttgctta gtgacaagcg tttgaattga aatacaaaat tagaataatc accaaaaagc
     1861 ttttcgctta taacaacaaa tattcttttt tttcaaagat gtatttattt ctttaccatg
     1921 gtattgttga catcgcagaa ttgattgcat atagttatcc gccttatcca gcattctggg
     1981 tatattcatt tggctaaata tttggtgtgt tatgaaaaga actttacaaa gaaagagcaa
     2041 actcacaaga gctagaacgc atggttttcg tttacgttcc agaacaaaaa atggaagaaa
     2101 agtgttagct aatcgtcgca gaaaagggcg taagcttctt gctccaactg gtggtaccac
     2161 aaaataagca acttgggggc ttaaccatta aattaggatt tcctcgctat cgtcgcttat
     2221 taaaacctga cgagtttaag agagtatttg gcaaggctaa aaaagtggcc tctgggcctt
     2281 ttattatctt ggcttgtcct aatcagcggg agtatcctcg attgggtgtg gtgctaagta
     2341 agaagaaaat tccaaaagca gtacagcgca ataggattcg acgcctaatt cgagaatgct
     2401 ttagaaagca aacagctctc ggaggggtcg atttcgtcgt attaccgaaa ccgggtatcg
     2461 atgtcttaag taatgcacag ttggagaaag ggttacttag agagtggcaa caatacacaa
     2521 cgtggtcaag caatcgctca ttaccttaat acgtggttac caatggtgca ttagtcccct
     2581 aatcggccca gcttgtagat tctatccaac ctgttctgaa tatgcgaaaa ctgctatttc
     2641 agattatggg gcaataaagg gaagttggtt aatttttaaa agattatgtc gttgtcatcc
     2701 cttgcatccc gggggatatg atccagtgcc aagaggaaca atcgaatgaa ggacgctatt
     2761 aatgcgcgag tgattttatt attaaccctc gtttttttgg ggatgacact ctatggtcgc
     2821 tggcaagaag aacacgctcc agtttctgta aatgaaccta aacaacaatc aacaaccccc
     2881 actacgcatg agccggatgt tccaaccgat atcccttttt ctgatgcacc tacgcagacc
     2941 actgcagctc cagtagaaac cgtacaggtt gatagattga taagcgttga aaacaatgtc
     3001 ttaaagctga aaatcgataa aaaagggggc gacattgttt atgcagagtt gaaagactat
     3061 cccaaggtaa aaggaaaacc agaacagggg tttgttttgc ttgataaaag caaagagcga
     3121 ttttatatag cacaaacagg gttaattgct aaatcaggtc cagactctag aaccttagga
     3181 cgtgctgagt atacctctca atttagtcat tatgatattt cttcct
//
LOCUS       DASTIB010000216         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1267947, whole genome shotgun sequence.
ACCESSION   DASTIB010000216 DASTIB010000000
VERSION     DASTIB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267947"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFP93_03765"
     CDS             <1..851
                     /locus_tag="VFP93_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9843756.1"
                     /translation="GKTTSVSNYTTDAYGFNVNYGIPMSEFNRLNFGVGVQSTRLKVG
                     SENELPIEIRNFLQSFQDPNSTHANTSENFQEVTVALGWSHNSLDRYVFPENGLSHGI
                     SSLVSAPGSDLQYYRLSYYLQYYKYLTHGFVGMLSTNLGYGNGYGKTKNLPFYKNFFA
                     GGSHSVRGFEESSLGPRDSRNNPFGGNFVATGTAALILPEFIAPGTRAVRTAVFVDAG
                     QAYFVHNRSDFAGPENPDELRYAAGVSLTWMTPMAPLVFSLATPLNEKPGDHVQKFSF
                     TFGTVF"
     gene            865..1392
                     /locus_tag="VFP93_03770"
     CDS             865..1392
                     /locus_tag="VFP93_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET9843757.1"
                     /translation="MMKRLLSALVLGSAVALAPMVHADKAPSKIGVLDVRQIIADSAE
                     TKSIEQALKKEYQPKIDEIQNLDKALRSNMEKLQRDQEVLSAAEKTKLEKKIKESQQE
                     LITKQNQYAQESAGKQQKMMQEFLDKVKKEVESFAQKEGYELILISDAVPYMSGKNDI
                     TSHIIKALKEKGSKS"
     gene            1408..2478
                     /gene="lpxD"
                     /locus_tag="VFP93_03775"
     CDS             1408..2478
                     /gene="lpxD"
                     /locus_tag="VFP93_03775"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET9843758.1"
                     /translation="MTTQKEFTLSELKNKFGGTIEGDSTVLIRGVGTVQSATASQIAF
                     VDNPKYQSHIPQSTAGAIIVSPNLKIETNKPLLITENPKLLFAKIVDYFHPKYVRDSG
                     IHPSAVIGKDVPIGKNVFIGAFCVIEEGVVIEDDVTLEPHCFIGRNAKIGKGSYLSAK
                     VTFYHHTQIGKYCHMHSGAVLGSDGFGFANDQGRWIKVPQVGRVVVGDHVEIGANTTV
                     DRGTLEDTIIEDGVIMDNLIHIGHNVRIGKFTAIAACCGIAGSCVIGAYCMIGGNSIL
                     NGHIQITDRCYFIPGSQVANSIKKPGVYSSGIPARDRNLWAKNVARFHQLDEMAGKIK
                     ALQKKIDAMRAVESLAKVEVKE"
     gene            2475..2921
                     /gene="fabZ"
                     /locus_tag="VFP93_03780"
     CDS             2475..2921
                     /gene="fabZ"
                     /locus_tag="VFP93_03780"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET9843759.1"
                     /translation="MTDTQLDINQILNYLPHRYPFLLVDKVLRYEDGKELVAIKNVTI
                     NEPFFTGHFPENAVMPGVLLVESLAQAGAVLAYITTRSNPRDSIFYLAALDNVKFKQI
                     VRPGDQLTLKVGILSQRSTFWKIYGEASVDNHVACTVEILSAMRKL"
     gene            2923..>3038
                     /locus_tag="VFP93_03785"
     CDS             2923..>3038
                     /locus_tag="VFP93_03785"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997004.1"
                     /note="catalyzes the addition of
                     (R)-3-hydroxytetradecanoyl to the glucosamine disaccharide
                     in lipid A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HET9843760.1"
                     /translation="MIADTARIDPSAKIAANVQIGHYSIIGPDVEIEKDCIIG"
BASE COUNT          969 a          537 c          656 g          876 t
ORIGIN      
        1 taggtaaaac cacaagtgtt tccaattata cgacggatgc ttatgggttt aacgtaaatt
       61 atggcatacc catgtcggaa tttaatcgtt taaatttcgg agtgggcgtt caatctaccc
      121 gattaaaagt agggtcagaa aatgaactac ccattgagat taggaatttc ttgcaatctt
      181 ttcaagatcc aaactccaca catgcgaata ccagcgaaaa ctttcaggaa gttaccgtgg
      241 ctctaggctg gtcgcacaat tcccttgacc gctatgtttt ccctgaaaat gggttatctc
      301 atggtatttc ttcactcgta tctgcaccag gttccgattt gcaatactat cgcttaagtt
      361 attatctgca gtattacaaa tacttaaccc atggttttgt aggtatgctt tccacgaatt
      421 tgggctacgg taatggttat ggtaaaacca aaaatttacc cttttataaa aatttcttcg
      481 caggtggttc acattctgta cgagggtttg aagaaagcag tcttggccca cgagattcga
      541 gaaacaatcc attcggtggt aattttgtgg cgacaggtac tgccgcttta atattgcccg
      601 aattcattgc acccggaact cgagccgtta gaacagcagt ttttgtcgat gcaggccaag
      661 cgtattttgt tcataacagg tcagattttg ctggtccaga aaatccagat gagcttcgtt
      721 atgcggcagg tgtatcgtta acttggatga cacctatggc ccctctcgtt ttttcattag
      781 caacacctct gaatgaaaag ccaggcgatc acgtacagaa attctcgttc acttttggaa
      841 cagtttttta acctaaggag cttaatgatg aaacgtttac tctcagcttt agttcttggt
      901 tctgcggtag ctcttgcgcc tatggtgcat gcagataaag caccaagcaa aattggtgtt
      961 ttagatgttc gacaaattat tgctgattct gcggaaacaa aaagtattga acaagcttta
     1021 aaaaaagaat atcaacctaa aatcgatgaa attcaaaatt tagataaagc tttacgatcc
     1081 aacatggaaa aattgcagcg tgatcaagaa gtgttgagtg ctgcagaaaa aactaagctt
     1141 gaaaagaaaa ttaaagaaag ccaacaagag cttatcacga agcaaaatca atatgcccaa
     1201 gaatctgcag gcaaacaaca aaagatgatg caggaatttt tggacaaagt gaaaaaagaa
     1261 gttgaatctt ttgctcaaaa agaaggttat gagttaatcc taatatcgga tgcagtccct
     1321 tatatgagtg gcaaaaatga tattacttct cacatcatca aagctttaaa agagaagggt
     1381 agtaaaagct aaagaggttg gcgcttcatg acaacgcaaa aagagtttac gctaagcgaa
     1441 ttaaaaaata aatttggtgg aacaatcgag ggagatagca cggtacttat ccgcggggta
     1501 ggaactgtcc aatctgctac agcttcgcaa atagcttttg tggataatcc gaagtatcaa
     1561 agccatattc cacaaagtac ggcgggtgca atcattgtaa gtcccaattt aaaaatagaa
     1621 acgaataaac ctttgcttat cacggaaaat cctaagctac tgttcgccaa aatcgtggat
     1681 tattttcatc caaaatatgt gcgtgacagt gggattcatc cgagtgcagt tattggaaag
     1741 gatgtcccga tcggaaaaaa cgtttttatt ggtgcgtttt gtgtgattga agaaggcgtt
     1801 gtcatcgaag atgatgtaac tctagagcca cattgtttta tcggtagaaa tgcaaaaata
     1861 ggaaaaggtt cttatttaag tgcaaaagtc accttttatc atcatacgca gattggcaaa
     1921 tattgtcaca tgcattccgg tgccgtttta gggagtgatg gatttggatt tgcgaatgat
     1981 caaggccgtt ggattaaagt tccgcaagtg ggtcgcgtgg tagtgggcga tcatgtagaa
     2041 attggcgcta acaccaccgt cgatagagga actttagaag ataccatcat agaagatggt
     2101 gtaattatgg ataatttaat acatattgga cacaacgttc gaataggaaa attcacggca
     2161 attgctgcgt gctgtgggat cgctggcagt tgcgttattg gagcctattg tatgatagga
     2221 ggcaatagta tactgaacgg gcatatccaa ataaccgatc gttgttattt tattcctggt
     2281 tcccaagttg ccaattccat aaaaaaaccc ggcgtatatt cttcaggaat tccagctcgc
     2341 gatcgaaacc tgtgggctaa aaatgtagca cgttttcatc aattagatga aatggctgga
     2401 aaaattaaag cgttacaaaa gaagattgat gctatgcgcg cagttgaatc attagcgaag
     2461 gtagaggtaa aagagtgaca gatacgcaat tagatatcaa tcaaatttta aactatttac
     2521 cgcatcgtta tccttttctt ttagtcgaca aagttttgcg ctatgaagac ggaaaagagt
     2581 tagtggcgat aaaaaatgta acgattaatg aaccattttt tacaggacac tttcctgaga
     2641 atgctgttat gcctggtgtt ttattagtag aatctttagc gcaagcagga gcagttctag
     2701 cttatattac tacccgctct aacccgcgcg atagcatatt ttatttagcc gcactcgaca
     2761 atgttaaatt taaacaaatt gtaagaccag gagatcaatt gacattaaaa gtcggtattt
     2821 tgagtcaaag gtccacgttt tggaaaattt atggagaagc atctgtagat aaccatgtgg
     2881 cttgtacggt agaaatctta agtgccatga ggaagctcta aagtgattgc tgatactgca
     2941 agaattgatc caagcgcaaa aattgctgct aatgttcaaa ttggtcacta tagtattata
     3001 ggtcctgatg ttgaaataga aaaagattgt atcattgg
//
LOCUS       DASTIB010000217         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1362722, whole genome shotgun sequence.
ACCESSION   DASTIB010000217 DASTIB010000000
VERSION     DASTIB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362722"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1999
                     /locus_tag="VFP93_03790"
     CDS             <1..1999
                     /locus_tag="VFP93_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9843761.1"
                     /translation="IEDAHSILWLTLNRADKSVNTFNEEVMRELDVFLNNVKQNKKIR
                     AIVIQSGKKTGFIAGADIEQFKTFQSANEATQLIQIGQKIFQKLEDLPQVTIAKIEGF
                     CLGGGLELALSCDYRIALESNKKTIGLPEVQLGIQPGWGGCVRLPDRIGAFKAFDLIL
                     SGRMLRPKEAKKMGIVDEAVPLRLLDKAVTEYATNKEKRAHTGWWPRITNFVAVRPLA
                     ARLFHYQVNKKVKKEHYPAPFQIINNWSKLGTQRPQAFTREAESIGELLVSNTSQNLV
                     RVFYLRERLKSLSRKTEYKPKHIHVVGAGVMGGDIAAWCALRGFHVTVQDKSPELLGS
                     CIKRTYELAKKQLKEKNLIQATLDRLLPDPEALGVHRADVILEAITEKLEVKQNFFKE
                     IEKKANKHAILATNTSTIPLEEIGAVLKHPERLVGIHFFNPVALMPLVEVVAGTQTSA
                     AFQDKAMAFVHAIDKLPLPVKSSPGFLVNRILLPYMLEAVLLLEEGISGPAIDKAAKD
                     FGMPMGPIELGDTVGLDVCLAACDKLSQSFGFTIPTKLKEQVLKGELGKKTQKGFYVW
                     KNGKPQKPKEQMQNLPEDIMNRMLFRMLNEAFTVLREGIVEEADLVDAGSIFGFGFPP
                     FRGGVLHYAKQRGIQTITDELQTLESRYGTRFAADKGVEVL"
     gene            2022..>2330
                     /locus_tag="VFP93_03795"
     CDS             2022..>2330
                     /locus_tag="VFP93_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9843762.1"
                     /translation="MGKYIIKKAAVLGAGVMGAQIAAFFVTAGIETLLFDLSGSKSDP
                     NSIAAGAINALKKLQPSPLGLKEEVNLIQPANYDHDLEQLKSCDFVIEAISERLEWKL
                     E"
BASE COUNT          787 a          407 c          501 g          635 t
ORIGIN      
        1 tattgaagat gcacattcta tactctggtt aactttaaat cgcgctgaca aatccgttaa
       61 taccttcaat gaagaggtca tgcgggagtt ggatgttttt ttaaataatg ttaaacaaaa
      121 taaaaaaatt cgagctattg tcatacaatc agggaaaaaa accggtttta tagcaggagc
      181 agatattgaa caatttaaaa cttttcaaag tgcgaatgag gcaacgcaac ttattcaaat
      241 aggccagaaa atatttcaaa aattagaaga tctaccccaa gtaacgatag cgaaaattga
      301 aggattttgt ttaggtgggg gattagaact tgcgttaagt tgtgattatc gaattgcatt
      361 agaatccaat aaaaagacca ttgggttacc cgaagttcag cttggcatac agccaggatg
      421 gggagggtgc gttaggttgc ccgatcgcat tggcgctttt aaagcattcg atttaatttt
      481 atcgggtcgc atgctccgcc ccaaagaagc aaaaaaaatg ggtattgtgg atgaagcagt
      541 acctttacgc ttgttagaca aagcagtaac agaatatgca acgaacaaag aaaagcgtgc
      601 gcatactggg tggtggcctc gtataacgaa ttttgtcgca gtacgtcctt tggctgcgcg
      661 tttatttcat taccaagtaa ataaaaaagt aaaaaaagaa cattacccag ctccctttca
      721 aattataaat aattggtcaa aacttggtac acaacgtcct caagccttta ccagggaggc
      781 agaatccatt ggtgaacttt tagtttcgaa tacgtcgcaa aatttagtac gtgtgtttta
      841 tttgcgcgaa agactaaaaa gcttaagcag aaaaacagaa tacaaaccca aacatataca
      901 tgtagtcggt gctggtgtta tgggtggaga cattgcagca tggtgcgctt tacgaggttt
      961 tcacgtcacc gtgcaagata aaagcccaga attacttggt tcttgcatca aaagaactta
     1021 tgagttagct aaaaagcaat taaaagaaaa aaatttaatc caagctacgc tggatagact
     1081 tttgcccgat ccagaagcat taggagttca tcgcgccgat gtaattcttg aagcaataac
     1141 cgaaaaatta gaagtaaaac aaaatttttt taaagagata gagaaaaaag ccaataaaca
     1201 cgcgatattg gctacaaata cttcaactat acctcttgaa gaaatcggtg cggtattaaa
     1261 acatccagag cgcttagtag gaattcattt cttcaatcca gttgctttaa tgcccttggt
     1321 agaagtcgtg gcaggtaccc aaacttctgc tgcgtttcag gataaagcaa tggcatttgt
     1381 acatgcaatc gataaactgc ccttgccggt aaaaagttca cctggatttt tagtgaatag
     1441 aattttattg ccttatatgc tagaagcagt gttattactc gaggaaggta tatctggacc
     1501 tgcgattgac aaagccgcaa aagattttgg catgcctatg ggccctatcg aattaggaga
     1561 cacggtgggt ctcgatgttt gtttggctgc gtgtgataaa ttatcacaat cttttggctt
     1621 tactataccc acaaaattaa aagaacaagt tcttaaaggg gaattaggta aaaaaacaca
     1681 aaaaggtttt tatgtgtgga aaaatggaaa gccccaaaag ccaaaagaac aaatgcaaaa
     1741 tttaccagaa gatattatga atcgaatgct ttttcggatg ttgaatgaag catttacggt
     1801 gctgcgcgag ggtattgtcg aggaagcgga tttggtagat gcaggatcca tttttggttt
     1861 tggttttcct ccctttaggg gcggagtatt gcattacgcg aaacaaagag gcattcaaac
     1921 cataaccgac gagcttcaga ctttagaaag ccgatatggc acacgtttcg cagctgataa
     1981 aggtgttgaa gtactctaat ttaattagaa ggaacgcatg tgtggggaaa tatattataa
     2041 aaaaagcagc cgttttaggc gcaggggtaa tgggagcaca aattgctgca ttcttcgtaa
     2101 ccgcaggcat tgaaacgctt ttatttgatc tatctggaag caaatcagat ccaaattcca
     2161 ttgcagcagg tgcaataaat gctttaaaaa aattacagcc atccccccta ggattaaaag
     2221 aagaagtaaa cctcatacaa cctgccaatt atgatcatga tttagaacaa ctcaaaagct
     2281 gcgattttgt catagaagct atttcggaac gattagaatg gaagctagag
//
LOCUS       DASTIB010000218         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_340072, whole genome shotgun sequence.
ACCESSION   DASTIB010000218 DASTIB010000000
VERSION     DASTIB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340072"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="secD"
                     /locus_tag="VFP93_03800"
     CDS             complement(<1..1136)
                     /gene="secD"
                     /locus_tag="VFP93_03800"
                     /inference="COORDINATES: protein motif:HMM:NF025098.4"
                     /note="part of the preprotein secretory system; when
                     complexed with proteins SecF and YajC, SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation, and appears to be required for the release
                     of mature proteins from the extracytoplasmic side of the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET9843763.1"
                     /translation="MNRFPLWKNILLLIILILGILYSIPNIFGEDPAVQILPNTGVTL
                     PTQLPTTLKNLFQEKNIDYKKIESDNDSLLIRFHDPNMQIKAKDLIKSVLQEEAIVAL
                     NLAPMTPKWLEFIGAKPMKLGLDLRGGVHFLLDVDVESAFQRRLESYVSDVKADLRDN
                     KIRWQDFEVHAKNYAFVLRFQDQETADEAYKLMRQNHQGLELTKSDDDNTTQIQATLA
                     ASLQQEISNYTVDQTISTLRNRVNELGVAEAVVQRQGLHHVVVELPGIQDTARAKDIL
                     GKTATLEFHMVNTEHDMREAIKGRTPPGNKIYYDKDKKPFLLNKRVLLTGESITGAVV
                     GTDAQGMPAVSIRVGGGGLNLFKKATRENIGKPMATVYLETKVD"
     gene            complement(1171..1533)
                     /gene="yajC"
                     /locus_tag="VFP93_03805"
     CDS             complement(1171..1533)
                     /gene="yajC"
                     /locus_tag="VFP93_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET9843764.1"
                     /translation="MFRRILFGLFMVSFSGMASADTAALTSGAGLGQFAILGVFIFFL
                     FWMMRSQNKRAKEHQNLINKLDKGDEVLINGGMVGKVTKVGDTFILVQFSEGFECLVQ
                     KNMIAASLPKGTIKSVKL"
     gene            1728..1814
                     /locus_tag="VFP93_03810"
     tRNA            1728..1814
                     /locus_tag="VFP93_03810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1762..1764,aa:Leu,seq:caa)
     gene            complement(1793..>2167)
                     /locus_tag="VFP93_03815"
     CDS             complement(1793..>2167)
                     /locus_tag="VFP93_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HET9843765.1"
                     /translation="DFTYIEDIIDGLLKVLENPPQKDNQWCADKPNPATSYAPYRLYN
                     IGNNHPIDLMDFVRAIEKATRKVAKINFMPMQNGDVVATHADISHLEKDFCYTPKTSI
                     QEGIEKFVEWYRDYYVGAGGET"
BASE COUNT          631 a          451 c          392 g          693 t
ORIGIN      
        1 tcgtctacct tcgtttctag gtaaacagta gccatgggtt ttccaatatt ttcacgcgtg
       61 gcttttttaa ataaattgag tccaccccca ccaacacgaa tgctaactgc aggcatgcct
      121 tgcgcatctg tccctaccac agcgcctgta atggattctc ctgtgagtaa aactctttta
      181 ttgagtaaga aaggtttttt atctttatcg taataaattt tattacccgg aggcgtgcgt
      241 ccttttattg cctctcgcat gtcgtgctct gtatttacca tgtgaaattc taaggtagcg
      301 gtttttccta aaatatcttt cgctctggct gtatcttgaa ttccaggaag ttctacgaca
      361 acatgatgta aaccttgacg ttgtaccacg gcttccgcaa cccctaattc atttactcga
      421 ttgcgtaacg ttgaaatggt ttgatctacc gtataattac taatttcttg ttgcaaactg
      481 gcggcaaggg tagcttgaat ttgcgtagta ttatcatcgt cactttttgt taattctaag
      541 ccttgatggt tttggcgcat gagcttataa gcttcatctg ctgtctcttg atcttggaaa
      601 cgcagcacaa atgcgtaatt ttttgcatgc acttcaaaat cttgccaacg aattttattg
      661 tcacgcaggt cggctttcac atcactgaca tagctctcaa gccgtctttg gaaagcgctc
      721 tcaacgtcta catccagcaa aaaatgcacc ccaccgcgta aatctaaccc taatttcatg
      781 ggttttgcac cgataaactc caaccatttt ggtgtcatgg gcgctaaatt taaggcaaca
      841 atagcctctt cttgcaatac ggattttatc agatcttttg ctttaatttg catattggga
      901 tcatgaaaac gaatcaacaa ggagtcattg tcagattcaa tttttttata atcgatattt
      961 ttttcctgaa aaagattttt taaagtagtg ggcaattgtg taggtaacgt cacaccggta
     1021 ttaggtaaaa tttgaacggc tgggtcttcg ccaaaaatat tagggattga atataaaatt
     1081 cccaaaataa gaataattaa aagtaggata tttttccaaa gaggaaaacg attcataccc
     1141 gatcccttat acgtttatag ggtgtttaaa ttagagttta accgatttga ttgtgccttt
     1201 tggtaaacta gctgcaatca tatttttttg aactaaacat tcaaaacctt ccgaaaactg
     1261 taccaaaata aaagtatcac cgactttagt gacttttcct accataccac cgtttattaa
     1321 aacctcatcc cctttatcta acttattaat aagattttga tgttcttttg cgcgtttgtt
     1381 ttgactacgc atcatccaaa ataaaaagaa aataaagaca ccgagaatag cgaattggcc
     1441 taagcccgct ccgctagtaa gcgccgcagt atctgcactc gccatacccg aaaaagatac
     1501 catgaataat ccaaatagaa ttctacgaaa catgtaatca ccaaaaaaag ttacaaaaca
     1561 aaattaaaca atgtgccctt taaagggcac gcatattcta gctttttgtg cttagttaaa
     1621 ccagtcagaa agcctgagat aagtaccgtt ggttatcaaa ctaatataaa tggtatctga
     1681 gaagcgtttg tgttcacaca aacctctgat ataatgcctc gcgattagcc ggagtggtgg
     1741 aattggtaga cgcgccagac tcaaaatctg gtgatggaaa catcgtgcga gttcaagtct
     1801 cgcctccggc accaacataa tagtctcgat accattcaac aaatttttct ataccctctt
     1861 gaatcgatgt ttttggcgta taacaaaaat ctttttctaa atgactaatg tctgcatggg
     1921 tagcaaccac gtccccgttt tgcatgggca tgaagttgat tttcgccact tttcgggttg
     1981 ccttttcaat agcacgtacg aaatccataa gatcaatagg gtggttatta cctatattgt
     2041 ataaccgata gggggcataa cttgttgcag gattgggttt gtctgcgcac cattgattat
     2101 ctttttgcgg tgggttttct aaaaccttaa gtaacccatc gatgatatct tcgatatacg
     2161 taaaatc
//
LOCUS       DASTIB010000219         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_956586, whole genome shotgun sequence.
ACCESSION   DASTIB010000219 DASTIB010000000
VERSION     DASTIB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956586"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFP93_03820"
     CDS             complement(<1..318)
                     /locus_tag="VFP93_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9843766.1"
                     /translation="MVKPQNIRILGIDPGSRVTGYGIIEGSANQWKVILSGCFILKGA
                     TYLERLQHLQHLLLALLSEYKPQECALEEVFLHKNANSALKLGQARGVVLANLLSFSI
                     SIFE"
     gene            complement(348..1076)
                     /locus_tag="VFP93_03825"
     CDS             complement(348..1076)
                     /locus_tag="VFP93_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9843767.1"
                     /translation="MAGHSKWANIKHRKGAQDAKRGKIFTRLIREITVAARLGGGDAT
                     SNPRLRTAIDKALASNMTRDTIDRAIKRGAGSEEADNLEEIRYEGYGPAGVAVMVDCL
                     SDNRNRTVSEVRHAFSKHGGNLGTDGSVSYLFNKLGQIIFAPGADEDKIMEVAIEAGA
                     EDVNIQEDKSIEVTTSAEAFDHVLGMLHEKQLKPAFAEVTMAPVTSIDLDADGSSSLT
                     KLIDALEDLDDVQEVYSNAQFPDN"
     gene            complement(1077..1508)
                     /gene="nudB"
                     /locus_tag="VFP93_03830"
     CDS             complement(1077..1508)
                     /gene="nudB"
                     /locus_tag="VFP93_03830"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HET9843768.1"
                     /translation="MLLKKPESVLVVVYTATGKILCLQRQDSTNFWQSVTGSLLEGES
                     PLAAATRELEEETGLTDPLPQALCYKTRWWFTIYPHWRYRYAPGVTKNLEHVFLCKIA
                     QECSVILSQEHLAYCWLDKKSALKKMSSDTNQRAIELFVEV"
     gene            complement(1498..>2214)
                     /locus_tag="VFP93_03835"
     CDS             complement(1498..>2214)
                     /locus_tag="VFP93_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET9843769.1"
                     /translation="GLEGLQSPIVKFLPENVVNQVLQRVKAQNGDIIFFGADKASVVT
                     EALGALRLKLGHDFSLAEKEWRPLWVVDWPMFGFNENTNHWDPLHHPFTCPVDENIEL
                     MKANPGKSKARAYDIVLNGVEIGGGSIRIYTPKMQSAVFDILGISKEEAEEKFGFLLE
                     ALQYGCPPHGGIALGVDRLVMLMTGAQSIRDVIAFPKTQTASCLLTGAPAPVPTAQLT
                     EVGIKLREKNLKEEEPSHTS"
BASE COUNT          594 a          528 c          430 g          662 t
ORIGIN      
        1 ttcgaaaatg gaaatagaaa aagataaaag attagccaac accacaccac gtgcttgccc
       61 aagcttcaag gcggaattcg catttttatg aagaaaaact tcttctaatg cgcactcctg
      121 tggtttatat tcgcttaata aagccaataa taagtgctgc aaatgttgga gtctttccag
      181 ataggtagca cctttcaaaa tgaagcatcc agaaaggata actttccatt ggtttgcgct
      241 gccttcaata atgccgtatc cggtaacgcg cgaacccgga tcaataccta agatacgaat
      301 attttgcggt tttaccaatt cgcgcttcag ataaatatcc ttatttttta attatctgga
      361 aattgtgcat tcgaatagac ttcttgcacg tcatcgagat cttctaatgc atcgattagt
      421 ttggttaagc tcgaactgcc atctgcatct aaatcgatgg aagtgacagg agccatagta
      481 acttcagcga atgcgggctt gagttgtttt tcatgtagca tgcctagtac atgatcaaat
      541 gcttccgcac ttgtcgtgac ctcaatggat ttatcttctt gtatgttaac gtcctcggca
      601 cctgcttcaa tcgccacttc cataatttta tcttcatcgg caccaggtgc aaaaatgatt
      661 tgccctagtt tattgaataa ataagaaacg gaaccatctg tgcctaaatt gcctccatgc
      721 ttgctgaaag catgtcgtac ttcggaaacg gttctattgc gattatcgct taagcaatcc
      781 accatgacag ccaccccagc agggccataa ccttcataac gaatttcttc aaggttgtct
      841 gcttcttcgc tcccggcacc gcgtttaatg gcacgatcga tagtatcacg ggtcatattt
      901 gacgcgagcg ctttatctat cgcagttcga agtcttggat tggaggtagc atccccaccc
      961 ccaagtcttg ctgccaccgt aatttctcta attaagcgcg taaaaatttt tccacgtttg
     1021 gcatcttggg cgcccttgcg atgtttaata ttcgcccatt tactatggcc tgccatctaa
     1081 acctccacaa atagttctat agcgcgctga ttggtgtctg aggacatttt tttcaaggcg
     1141 ctttttttat ctaaccaaca atacgctaaa tgctcttgcg ataaaattac ggagcattct
     1201 tgcgcaattt tacagagaaa aacgtgttcg agattttttg ttacacccgg cgcataacga
     1261 tagcgccaat ggggatatat cgtaaaccac caacgcgttt tgtagcacaa agcttgcggc
     1321 aaaggatcgg taaggcctgt ttcttcttct aattctcttg tggcggcagc aagaggtgat
     1381 tccccctcta gcaaacttcc cgtaaccgat tgccaaaagt tcgtgctatc ttgacgctgt
     1441 aaacaaagaa ttttaccggt agccgtgtat accactacca gtaccgattc cggtttttta
     1501 agaagtatgc gagggctcct cttcctttag gtttttttca cgtaatttta tgcctacttc
     1561 ggtcaactgc gcagtcggaa caggtgccgg agccccagta agtagacaac ttgccgtttg
     1621 cgttttggga aatgctataa catctcgaat actttgtgcg ccggtcatta acatcacaag
     1681 acgatcaaca cctaaggcaa taccaccatg aggtgggcac ccatattgca atgcttctaa
     1741 taaaaagcca aatttctctt ctgcttcttc tttactgatc cccaaaatat caaacacggc
     1801 agattgcatt ttcggcgtat aaattcggat agatcccccc cctatctcta caccgttgag
     1861 aacaatatca taagcacgtg ctttggattt acccggattt gctttcatta actcaatatt
     1921 ttcatccacc ggacacgtga aaggatgatg caatggatcc caatggtttg tgttttcgtt
     1981 aaaaccgaac ataggccaat ccactaccca taacgggcgc cattccttct ctgctaaaga
     2041 gaaatcatgg cctaatttca atcgtagtgc gcctaacgct tctgtgacta cacttgcttt
     2101 atctgcacca aagaagatga tgtcaccatt ttgtgccttc acgcgttgta aaacttggtt
     2161 tactacgttt tcaggtaaga attttacaat tggggattgt aagccttcca agcc
//
LOCUS       DASTIB010000220         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_436167, whole genome shotgun sequence.
ACCESSION   DASTIB010000220 DASTIB010000000
VERSION     DASTIB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436167"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFP93_03840"
     CDS             <1..370
                     /locus_tag="VFP93_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843770.1"
                     /translation="MQQEELLLEEAHRILKKSGIIRLDGLNVFCVGHMKEYLVKKFLR
                     KAEHLHFSNPWRVKQLLHNKQFTCIEIYWLVLLPKSLRKFQQIVLTAKAQKYLQKLYW
                     ITMWFSHTFYVQATKDNHAS"
     gene            360..1292
                     /locus_tag="VFP93_03845"
     CDS             360..1292
                     /locus_tag="VFP93_03845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9843771.1"
                     /translation="MLHKVLNYVLPHGRFASLLIILYHRVVWQSDPLNTDILHREKFK
                     AQIQFFKQFYQIMPINEGISRLLEGSLPYNALCITFDDGYQDNVDIALPILKSYKIPA
                     TFFIATGFLQGKLMFNDYITESIRGFHERLRCGEHQFDCSTLAGKRLAINFFINKIKN
                     LAHQERIAYLKDFLPVTSIKPLMMTEEDIQTLSKEKMLIGAHTVNHPVLSKEDDNTAY
                     EEIKASKNYLQNIIQSEIQWFAYPNGKWQQDFDQKHCEMVKNLGFMGAFTTDRAYAKR
                     AMDPFRIPRFTPWGRTPLKMFRQLSFYYFKNAFI"
     gene            2639..>3569
                     /locus_tag="VFP93_03850"
     CDS             2639..>3569
                     /locus_tag="VFP93_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843772.1"
                     /translation="MTKVITKQDILDLENDLRAEMIKKLNPSEKWNYCTFLPGDNQQA
                     IKDQMLKFLNNDLGSFLQASSDILVNFTQADADSIGSGKETSNALKFIDLMKGLEKSG
                     FNGTRNMPINFWSGNIGRTRAHLNPQQEFSDSEVTSLVVLFDLCGLIQKFESKSAQQD
                     PNFKKTLSSLLPEALSKLYANEARGNVNVYMASDKLSELSGLTAGNNFWNAELPTLQS
                     LLHLGKVKAILVHTYDHATKSWKSPVNINDREADVINIVRRCSHPGSGKLPADTDPQL
                     FVQDRSQKEYEVWCNTASARSLIQLGKLRKFTSK"
BASE COUNT         1148 a          678 c          645 g         1098 t
ORIGIN      
        1 tatgcaacag gaagaattgc ttctcgaaga agcgcataga attcttaaaa aatcaggtat
       61 tatcagactc gatggattga atgtattctg tgtgggtcac atgaaggaat atttggttaa
      121 aaaatttttg cgcaaagcag agcatttgca tttttctaac ccgtggagag ttaaacaatt
      181 acttcataat aagcaattta cctgcataga aatatattgg ttagtattgt tgccaaaaag
      241 tttacgaaaa tttcagcaaa tagttctgac tgcaaaagcc caaaagtatt tacaaaaatt
      301 gtattggatt acaatgtggt tttcgcatac gttttatgtc caggcaacaa aggataatca
      361 tgcttcataa agtattaaat tatgtattac cgcatggaag attcgcttca cttttaatta
      421 ttctttatca tcgcgttgta tggcaaagtg atcccttaaa cacagatata ttacaccgcg
      481 agaaatttaa agcacaaata caatttttca aacaattcta tcaaattatg ccgattaatg
      541 aaggtatttc tcgcttgtta gaaggcagct tgccctacaa tgcactttgc ataacctttg
      601 atgatggtta tcaagataat gttgacatag cgttgcccat attaaaatcc tataaaatac
      661 cagcaacttt ctttattgcc acaggatttc tgcaaggtaa gctcatgttt aacgattata
      721 ttacagagag tatacgaggt ttccatgaac gtttaagatg tggcgaacat caatttgatt
      781 gttcaacatt agctgggaaa cgcttggcta tcaatttttt tataaacaaa attaaaaatt
      841 tagcacacca agagcgcatt gcgtatttaa aagatttttt acctgtaact tcgataaagc
      901 cgctaatgat gactgaagaa gacatccaaa ctttgagcaa agaaaaaatg ttaataggcg
      961 cgcatacggt caatcacccc gttttatcta aagaagacga taatactgct tatgaagaaa
     1021 ttaaagcttc aaaaaattat ttacaaaata ttattcagtc tgaaatacag tggtttgcgt
     1081 atcccaatgg aaaatggcaa caagactttg atcaaaaaca ttgtgaaatg gtaaaaaatt
     1141 tgggatttat gggggcgttt actaccgatc gtgcctacgc aaagcgtgct atggatcctt
     1201 ttcgtattcc aaggtttacc ccttggggta gaacgccgct taaaatgttt cgacaactat
     1261 ctttttatta ctttaagaat gcatttattt aactaataaa taaagagtaa tgcatttttg
     1321 tgcattctgc tcttgtatcc gcatacgcgc gatacctcaa aggccgtgct cgcgaatatg
     1381 cgtttcctgc cattcccccg agcgcttatt ttcctcattc cccaaacact tcccttgtca
     1441 tttccgcaaa cgaatatctc gtcattcccg cgaacgctga agctattatg aaagttataa
     1501 ggttacttaa agttaacaga gtacaaattc tccatgcgga cgcttaaata caaccctgta
     1561 acgcttcgca agcgacatcc tgtcgtttga ggcagcttag agtattttac tctgttaact
     1621 tcataaattt tataaagatt tatgctatgg caaaaccggt cctcgtcttt cccgtgaacg
     1681 cgggaaacca tcataagtac tcaactttgt gtggagttag cagaacaatt tctccatgcg
     1741 gacgcttaaa tacaaccctg taacgcttcg caagcgacgt cctgtcgctt gaggccgctt
     1801 ggagtatttc tactctgtta actttcataa attttataaa gattacttat gctatgcaaa
     1861 aaaggttctc atctttcccg tgaacgcggg aaaccatcat aagtactcaa ctttgtgtgg
     1921 agttagcaga acaatttctc catgcggacg cttaaataca tccctgtaac gctccgcaag
     1981 cgacgcactg tcgcttgagg ccgcttggag tatttctact ctgttaactt tcataaaatt
     2041 tacaaagatt acttaagctt atgcaaaaaa gggttctcgt ctttcccgcg aatgcgggaa
     2101 accatcataa gtactcaact ttctgtggag ttagcagaag aatttctcca tgcggacgct
     2161 taaatacatc cttgcatcgc tctgttaact ttcatagtta ttacgcaata ttttctctcc
     2221 tcattcccgc aagcttcccc tcgtcattct cgcaagcgcg atcaatctta tcaaaattac
     2281 ttatttaagt aaggatgagt tgtcttgctc gcaaaagcac gtcaaagggc ccaaagcggg
     2341 aaggtagctt aattcggtaa agtgccaatt ttcgtttctc aaagtattta cgttacattc
     2401 tgatcttttt tctagattat ttaggcttag tctcatcgtt aaaaagcgaa caatttgcca
     2461 tacattctca tacatctatg gaataccttt tggagcatct attattttca cgaacaagcg
     2521 tttcaactac caattatctc ccgatgaacg gactaaaata cgtaatttat ttaggacgtc
     2581 acaaaatttt ctaaatcgta caattagcca tcggttaaat aatcaaatgg tgatgtttat
     2641 gacaaaagta attacaaagc aagatatact tgatcttgaa aatgatctaa gagcagaaat
     2701 gataaaaaaa ttaaatccaa gtgaaaaatg gaattattgc acgtttttgc cgggggataa
     2761 ccaacaagcc atcaaggacc aaatgcttaa attcctcaat aacgatttag gctcattttt
     2821 gcaagcgagt tctgatattt tagtaaactt tacccaagca gatgcagata gtataggctc
     2881 tggaaaagaa acttccaacg cgctgaaatt cattgaccta atgaaagggt tagaaaaaag
     2941 tggatttaac ggtacaagaa atatgcctat aaacttttgg tctggtaata taggtcgaac
     3001 tagagcgcac ctaaatccgc agcaagaatt ttcagattct gaggttactt ccttggtggt
     3061 attattcgat ttgtgtggtt tgattcaaaa atttgagtca aaaagtgcac aacaagaccc
     3121 caattttaag aaaacgttat cgagtttatt acccgaggca ctttcaaagc tatatgctaa
     3181 tgaagcacgg ggtaatgtca acgtttatat ggcatccgat aaactaagtg aactttccgg
     3241 gttaactgca ggtaataatt tttggaatgc ggagttacca actttgcaat cattattaca
     3301 cttgggaaaa gttaaggcta ttttagtaca tacttatgat catgcgacta aaagttggaa
     3361 atcgccagtt aatattaatg atcgtgaagc agatgttata aatatagtaa gacgttgctc
     3421 tcatcctggt agtggtaaat tacctgctga tacggatccg caactatttg ttcaagatcg
     3481 cagccaaaaa gaatatgagg tgtggtgtaa cacagcttca gcacgatctc taattcaatt
     3541 gggcaaatta agaaaattta ccagcaaat
//
LOCUS       DASTIB010000221         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1449885, whole genome shotgun sequence.
ACCESSION   DASTIB010000221 DASTIB010000000
VERSION     DASTIB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449885"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1969
                     /locus_tag="VFP93_03855"
     CDS             <1..1969
                     /locus_tag="VFP93_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9843773.1"
                     /translation="IKQEPDAANSVLLIAPRVIMHSLDLPKTKILQQGSRLSYQLWIA
                     GDRSSVEKYHQWLQAQHNLNWRIDTLKQGRSPIQRLFSYAQDYLGLIVLLVVIMSGLS
                     IQLATRFWIQERVQIISLYKVLGATPVQVVRIFALGLMSWSVLICIIFIFLGVLLCYV
                     FLLYLQTHYTIISVKISIYPILLSFFICIGLMLGFSLPPLWSAKHLLPSLVFRETLVK
                     HKTTWVFYSLSLAFCLSLLYFYVQQVTLTLYVIAGIGWAAFIAFMVLWAILIGLSKIP
                     TKPFVIRVSLRYLHKNLTNSAFQVVAFTLVLITMIVLYGIRGDFLQQWKAQLPHDTPN
                     YFLINIAPENVPPLTTLIQDHLQQSAQFYPIVRGRLTHINSRPVTSELEDNSQESIQR
                     PLNLTFGDAIPSDNQVVQGGAWNASLQNKPLISLERSLSHRLKVTLGDTLSFFIDGQT
                     ITGTIYQIRNVQWQSFRPNFFVIFPQGVIDNFPSMYITSFYIPDNKKDVLLTIHNTFP
                     EITTIDIEKIIKEIQYMMNIMIHGIESLIWIIFVFAFIIMATILMTTKPQRKKNVALM
                     KLLGTTRKNIILIIAFEYFLMGFIAGLVAISASIWIEYQLAHAIFDVNYHIPWFRLVI
                     FWIGSMIVLGLFGSISLKDVINQSPLLLLKS"
     gene            1988..>2569
                     /locus_tag="VFP93_03860"
     CDS             1988..>2569
                     /locus_tag="VFP93_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET9843774.1"
                     /translation="MIKEIRQIFLNGLKVFLPIVITFAIVIWIFYYIELFFGNIIRIF
                     IPDRYYFDGLGIIIGVLFVFILGVLLRAWIITKLYKLAEKFVKKIPLVKTIYGWVKDL
                     LTYFEKAQTSEEHQAVVVRFGKLRLLGFITRESFQGLPPEFGDNDHVLVYLPMSYQIG
                     GYMLSIPRDRVTPINLKVNKALPLIITAGMSGVD"
BASE COUNT          770 a          411 c          472 g          916 t
ORIGIN      
        1 tataaaacaa gagccagatg ctgcgaatag cgttttatta atagcacctc gtgtcattat
       61 gcatagttta gatctgccga aaactaaaat tttacaacaa ggaagtaggc ttagttatca
      121 attgtggatt gcaggcgata gatcatcggt agaaaaatat catcaatggt tacaggcgca
      181 acacaatcta aattggcgta ttgatacgct aaaacagggt agatctccta ttcagcgcct
      241 attttcttat gcccaagatt atctcgggtt aatcgtactc ttagtcgtta taatgagtgg
      301 cttaagcatt caattggcta cccgtttttg gatccaagaa cgggttcaaa tcattagttt
      361 gtataaggtt ttaggagcaa ctccggtaca agtcgtacgt atatttgctt taggtctaat
      421 gagctggagt gtgttaattt gtataatttt tatttttttg ggtgtacttc tttgctatgt
      481 ttttttgcta tatcttcaaa cgcattatac tattattagt gtgaaaataa gtatttatcc
      541 aattctatta tcatttttta tttgtatagg attaatgtta ggattttcat tgccaccctt
      601 gtggtctgca aaacatcttt tgccgagttt ggtttttcgt gaaacgcttg taaaacataa
      661 aaccacctgg gtgttttatt cgttaagctt agcattttgt ctcagtttac tgtattttta
      721 tgtacagcaa gttacattaa ctttatacgt aattgcaggt attggttggg cagcttttat
      781 agcgtttatg gtattatggg ctattttaat tggtttatct aaaataccaa caaagccatt
      841 tgtcatacga gtaagtttac gttatttaca caaaaattta acaaatagcg ctttccaagt
      901 ggtagcattc acgttggtgc tcattacaat gattgtttta tatgggatcc gtggcgactt
      961 tttacagcaa tggaaagcac aattaccgca tgataccccg aattattttt tgatcaacat
     1021 tgcacctgaa aatgtacccc ctctcacaac gttgatacaa gaccatcttc aacaaagcgc
     1081 ccaattttac ccgattgtgc gtggccgatt aacccacatt aatagtcggc ccgtaacgtc
     1141 agagctagaa gacaattctc aagagagtat ccagcgtcct ttaaatttaa cttttggaga
     1201 tgcaataccg agtgacaatc aggtggtgca aggtggcgca tggaatgctt ctttacaaaa
     1261 taaaccgctg atatcgctgg agaggagttt aagtcataga ttaaaagtaa ccttgggaga
     1321 tacactttcg ttttttatcg atgggcaaac cataaccggc acaatttacc aaattcgcaa
     1381 cgtacaatgg caatcattca ggcccaattt ttttgttatt tttccgcaag gggttatcga
     1441 taattttccg tctatgtata ttacaagctt ctatattcca gacaataaaa aggatgtatt
     1501 attaacaata cataatacgt ttcctgaaat aacgacaatt gacattgaga aaattattaa
     1561 agaaatacaa tacatgatga atataatgat tcacggtatt gaatctttaa tctggattat
     1621 attcgttttt gcttttatca ttatggcaac tatccttatg acaacaaaac cgcaacgtaa
     1681 aaaaaatgta gcactcatga aattattagg aacgacacgc aaaaatatta ttttgattat
     1741 tgcttttgaa tatttcttaa tgggttttat agcaggactc gttgccataa gcgcaagtat
     1801 ttggattgaa tatcaattag cgcacgcaat atttgatgtg aattatcata ttccttggtt
     1861 tagacttgta atattttgga ttggtagtat gatagtcttg ggtttatttg gaagtataag
     1921 tcttaaagat gtaataaatc aatctccttt attattatta aaatcgtgaa ttatacagtg
     1981 agattttatg atcaaagaaa taaggcaaat atttcttaac ggattaaagg tttttttacc
     2041 tattgtcatc acatttgcaa ttgttatttg gattttttat tacattgagc ttttttttgg
     2101 taatataatt cgcatcttta ttcctgatcg ttattatttt gatggcttag gaattattat
     2161 tggtgtttta tttgttttta tattaggtgt tttgctgcgt gcttggataa taaccaagtt
     2221 gtataaattg gccgaaaaat ttgttaaaaa aattccctta gtaaaaacga tatatggttg
     2281 ggtcaaagat ctacttacct attttgagaa agcccaaacc tcagaagaac atcaagcggt
     2341 ggtagttcgg tttggcaaat tgcgcttatt gggttttatt acgcgagaat cttttcaggg
     2401 attaccacct gaattcggtg acaatgatca tgttctagtt tatttaccca tgagttacca
     2461 aattggtggt tacatgcttt ctattccgcg cgatcgtgtt acacctatca atcttaaagt
     2521 gaataaagct ttgccgctta ttattacagc gggtatgtcg ggtgtagat
//
LOCUS       DASTIB010000222         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_749136, whole genome shotgun sequence.
ACCESSION   DASTIB010000222 DASTIB010000000
VERSION     DASTIB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749136"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFP93_03865"
     CDS             <1..192
                     /locus_tag="VFP93_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843775.1"
                     /translation="LILIGIVNQPAITGLIGSFTPAFQFYNVTQTVNDLGLMGIASFI
                     VLITGALGAMMGAIFGLKR"
     gene            295..654
                     /locus_tag="VFP93_03870"
     CDS             295..654
                     /locus_tag="VFP93_03870"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9843776.1"
                     /translation="MELPHDLVKAGHQVIGVDVKNNEGEDLGCIKEIILEKVEGNVRY
                     IVLECGSFLGLKGKYFAIPWNSIDYDKDEECFRLDIKKEDFKNAPGFDKDHWPNMADT
                     NWGKQVHAYFKSKPYWE"
     gene            complement(702..1319)
                     /locus_tag="VFP93_03875"
     CDS             complement(702..1319)
                     /locus_tag="VFP93_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843777.1"
                     /translation="MTQTKSSNAARNLRSKKSSTAMRSKSSTAKSKTKSAKGGLGKKT
                     SARGMKKSTSTSSRMGTTGKTSRTTSSKGTSSSGTRKTASSKGMGLSATSKKKTSTTG
                     RSTKSTTRGLSAGRGAKKGMGGDQEFAGTSMKKGTSKTAPKRSTRGQTSKGKSSRKST
                     ESRMGSTRSAAGGGKAKGRGRSISKTSGRIGSSRRSATRTTRPSI"
     gene            complement(1733..2188)
                     /locus_tag="VFP93_03880"
     CDS             complement(1733..2188)
                     /locus_tag="VFP93_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843778.1"
                     /translation="MTNIQKTTQQGRISNKTANKKKNTLKGSLKAKGRTTKQGDKRAV
                     VKKVKSIRTQEGKNFEGKIGDVRKRTNTKRDASKELSANEEKKSRKGKGPSSELQRER
                     AAKKKKAQSPAQHFKEGKNRVKTKIPKTSRPNTSVHKKPSKRSSSSSKH"
     gene            2409..2636
                     /locus_tag="VFP93_03885"
     CDS             2409..2636
                     /locus_tag="VFP93_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843779.1"
                     /translation="MVDKNDMNEDDDLTSTNDMDDDEEIGMGNKQKQGFGTQKQGQQN
                     QQGYGSQKPGQGQGSSQTSQKPNPKGQSGKL"
     gene            2692..3138
                     /locus_tag="VFP93_03890"
     CDS             2692..3138
                     /locus_tag="VFP93_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9843780.1"
                     /translation="MDAIALLLKDHQRLKKLLKQLVNTSQRATQKRHELFTIIRFEAK
                     LHETMEEKFLYKHLREMKQSFMNAFEHTEEVSLMNHVLLKMVNSDFSKDAWSAKANVL
                     MEISANHIAKEEDEKFPQAIKLFTQQDLDQIGAQMRRFKERHSIKK"
     gene            3227..>3757
                     /locus_tag="VFP93_03895"
     CDS             3227..>3757
                     /locus_tag="VFP93_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET9843781.1"
                     /translation="MRYLNFSLFWLLLLNHSTIVVAHNLPLEKIKLPPGFSITVYAEN
                     VKNARQMALGTQGTLFVGSKKAGKVYAITALTPGKKSTEVRTIADNLNLPSGIAFKDG
                     ALYIAEVSRILRYDNIEENIHNPGKPKVIYDKLPKEQHHGWKFIGFGPDNLLYVPVGA
                     PCDNCLSHDPRFASLLR"
BASE COUNT         1080 a          653 c          730 g         1294 t
ORIGIN      
        1 ttaattctta ttggtattgt taatcaacct gccatcacag gtttgattgg gagtttcacg
       61 cctgcttttc agttttacaa tgtaacccaa acggttaatg atttaggctt gatgggaata
      121 gcaagtttta ttgtattaat cacaggagca ctgggtgcaa tgatgggtgc aatatttggt
      181 cttaaacgtt aacaaatttt aaaagttcac gatggagaaa ggattaagct aaactagaaa
      241 cgttcgtttt tcgataaaac caatcactat ctgtttatga aaccaggagg gtttatggaa
      301 ctaccacatg atctcgtaaa agcaggacat caggtaatag gtgttgatgt taaaaataat
      361 gaaggtgaag atttaggttg cataaaagaa ataattttag aaaaagtgga aggtaatgtc
      421 cgttacattg ttttagaatg cggcagtttt ctgggactta aaggcaaata ttttgctatt
      481 ccatggaatt ccatagatta cgataaagat gaagaatgtt ttcgacttga cattaaaaaa
      541 gaagatttca aaaatgcacc tggttttgac aaagatcatt ggccaaatat ggcggatact
      601 aactggggca agcaagtaca tgcttatttt aaatcaaagc cctattggga ataaaaaaat
      661 gagaacgcgt aatgtgttct cattttttac ttttaaggtt tttatataga aggacgagtt
      721 gttcttgtgg cagatcttct ggatgaacca attcttcctg aagttttaga gatactgcga
      781 ccacgtcctt tagctttgcc accgccggca gcagaacggg ttgaacccat gcgactttcg
      841 gtggatttcc tactgctttt acccttactg gtttgaccac gggttgaacg tttaggagca
      901 gttttggagg ttcctttttt catggaagtt ccagcaaatt cttgatcacc acccatacct
      961 tttttagcgc cacgaccagc acttaaacct cgagttgtgc ttttagtgct tcttcctgtg
     1021 gtacttgttt tctttttgga agttgcactg agtcccatgc ccttactgct agctgtttta
     1081 cgcgtaccgc tagagctagt tcctttactg gaagtagttc ttgacgtttt tcccgttgta
     1141 cccatgcgag aggaagtgct tgtgcttttt ttcatgccgc gagcacttgt ttttttccct
     1201 aatccacctt ttgcactctt agtttttgat ttagctgtgg aggatttaga tcgcattgca
     1261 gtactcgatt ttttagatct gagatttcga gctgcgtttg aacttttcgt ttgtgtcata
     1321 gaggtctccg ttagacgaat cattaattac aaacttaata taacgaatta cgttataagt
     1381 gtataggggt ttatgcaaat gctgtaaact tttttcacct aattttaaat gacacaccga
     1441 ctatgatgca aatgaacatc ctttatcttt tttttctttc taatttttct ttttcatttt
     1501 ctgttttgcc cccatgcgtt tccccagttg gcgacctctt tctgcttgcg gtatagcaat
     1561 tttctttgat ttcgatcggg gatgattact tccggtatct tgatttttta cttgtccttg
     1621 tggttgtgtt ttaaaacgtt ctgcttctgt gtctcttttt tacttgtgtt aaattttcgt
     1681 acatattcat cgttgtgttt cttttacata gggtccttaa tgaatttcct gatcaatgtt
     1741 tgctgcttga actggatcgt ttactaggtt tcttatggac ggaagtatta ggtcttgatg
     1801 tttttggtat tttcgttttg acacgatttt tgccttcttt gaaatgctga gcaggggatt
     1861 gtgctttttt cttttttgct gctcgttccc tttgaagctc cgaagatgga cctttacctt
     1921 ttcgagattt tttttcctca ttggcggaaa gttctttgga agcgtcgcgt ttggtattgg
     1981 tacgtttacg aacatctcct atcttgccct caaaattttt accctcttgt gtgcggatcg
     2041 atttaacttt ttttacgact gcgcgcttat cgccttgttt tgtggtacgt cccttggctt
     2101 tcagcgaacc ttttagggta ttcttttttt tattagcagt tttgttagaa atgcgacctt
     2161 gttgcgtagt cttttgaata tttgtcatga tgattatcca ttattttctt aacattataa
     2221 tagctcaaat tagaataaat tacagagcgc ttttttatta ttactattct tctaagcaga
     2281 tgaattttaa tttccattgg aaatacctat aaaatattca acaatatgca cgtatttgct
     2341 ttctttaatc aaagagtcta ggattggtat tgcaaattaa cttggcttag aaattggagg
     2401 atatttttat ggttgataaa aatgacatga atgaagatga tgacttaacc agcaccaatg
     2461 acatggacga tgatgaagaa attggtatgg gtaataaaca aaagcagggg tttggaacgc
     2521 aaaaacaagg acaacaaaac caacaaggct atggtagtca aaagccagga caaggtcaag
     2581 gatccagtca aacttctcaa aaacctaatc caaaaggtca aagcggaaaa ttataaaaac
     2641 tttataagag aaatacgtcg tatttctctt atttaatttg taggaacaat tatggatgcc
     2701 attgcattac ttttaaaaga tcatcaacga ttaaaaaaat tgcttaaaca attagtaaat
     2761 acttcgcaaa gagccacgca aaagcgtcat gaattgttta cgattatacg ttttgaagcc
     2821 aaattgcatg agactatgga agaaaaattt ttatataaac atttgcgaga aatgaagcaa
     2881 agttttatga acgcattcga acatacggaa gaggtttctt taatgaacca cgtactgcta
     2941 aaaatggtta attccgattt ttcgaaagat gcatggtctg caaaagcaaa tgttcttatg
     3001 gaaattagtg ccaatcatat tgcaaaggaa gaagatgaga aatttccaca agcaataaaa
     3061 ttattcacgc agcaagattt agaccaaatt ggagctcaaa tgcgacgttt caaagaacgt
     3121 cattctatca agaaataaaa caacattagg ttcattctaa tccgattatg tctacctata
     3181 gggacatgcc ataatagtaa gaactaagat cacccggagg atttaggtga gatatttaaa
     3241 tttttcacta ttttggttat tgttattgaa ccattccaca atcgttgttg cacacaattt
     3301 gcccctcgaa aaaattaaat tgccacccgg gttttccata actgtttatg ccgaaaacgt
     3361 aaaaaatgct cgccaaatgg ctttaggtac gcaaggtaca ttatttgtcg gttccaaaaa
     3421 ggcgggtaag gtttacgcga taacagcctt aactcctggg aaaaaaagca cagaagtaag
     3481 aacaattgct gacaatttaa atttgccctc gggtattgct tttaaagatg gtgctttata
     3541 tattgcggaa gtttcgcgta tattacgtta cgataatatt gaagaaaata ttcacaatcc
     3601 gggaaagccc aaagtaattt atgacaaact accgaaagaa caacaccacg gttggaaatt
     3661 tatcggtttt ggtcctgata atttacttta tgtgcctgta ggtgcccctt gcgataattg
     3721 tttatcgcat gatccccggt ttgcttctct cctgcgc
//
LOCUS       DASTIB010000223         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_868641, whole genome shotgun sequence.
ACCESSION   DASTIB010000223 DASTIB010000000
VERSION     DASTIB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868641"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1621
                     /locus_tag="VFP93_03900"
     CDS             407..1621
                     /locus_tag="VFP93_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843782.1"
                     /translation="MKDKPSNALFKKKRKKSKDPAWSKRDEKRKKSATTHAKAFSSIA
                     QLNKILVSPAKQVKIVTEKGIHIAGSLCLDDLCQENLTLSTPTKGAVSSALLSNNNEK
                     LLRARTIKTHQGKVSIFLSEDKVDKDAYVKLPAPPQNLANLLKSINFPQHTNALGFSD
                     IEITLESLKAIRSQKRKHVIGGSSERQPTNAQVVHYNSANSIAKLLGLEGNDWQWWHL
                     VGHALQGDEAQNIENFVLGKLHENSKMNDLAEDLVEKILVKLKKLGQNPKLYLSVTPE
                     WVPGFEMARLAKKVTWTLKDSPTQNPKHCAIFEFDPLSTDVLSKSEQESVEKVLMHIF
                     LADIPVEVVSPGKKPKSTLLLSPAKNPKSFDMQPTNDFPKKNLLALFEKEQINLNSSL
                     VQSNQTKKNKIR"
     gene            1731..>2301
                     /locus_tag="VFP93_03905"
     CDS             1731..>2301
                     /locus_tag="VFP93_03905"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9843783.1"
                     /translation="MDFHPNILSAIGNTPLIKLNKVVDKKSATVLVKCEFMNPTGSIK
                     DRMASYIIEDAEKKGLLKPDGTIVENTSGNTGLSLAMVAAVKGYRCIFTMPDKMSIEK
                     INMMKAFGAQVIVTPTDVPGDSPDHYVNTARRIAQETHNGFYVNQYHNRANIDAHYYS
                     TGPEIWRQTQGKLDYFVAGTGTGGTISGVG"
BASE COUNT          797 a          415 c          427 g          662 t
ORIGIN      
        1 taattccagc ttttaagcct ggtcatttca ttgaactcga tggattagtg aaatactata
       61 ctattattcg tgtatatgat ggtttagaac agtttacgat cttattttta atgcaatcgc
      121 tttaaactcg aaggtgtagt gctaaagcac gcaatgggta aggctaaaca ttaaaacccg
      181 gttttacttt taaactattg tactttcggt agtattgtcg cagggcatac gtttgagccc
      241 caaaaagctt cttttgaggg catcaaaaaa ataccattgg agatctttgc gataataatg
      301 caattacacc cttaaaaact acgaaacctt cgatccatcc gtaaaatgtt gctttgctcc
      361 attaacaagg tacaataatc cctttagagc tcttattcga tttttcatga aagataaacc
      421 ttctaacgct ttatttaaaa aaaagcgtaa aaaatcaaaa gatcctgctt ggtctaagcg
      481 tgatgagaaa agaaaaaaat ctgcgactac gcacgctaaa gcatttagct ctattgcaca
      541 actcaacaaa atattagttt cacctgccaa gcaagtaaaa atcgttacgg aaaaaggtat
      601 tcatatcgca ggatccttgt gtttagatga cttatgccaa gaaaatttga ctttatctac
      661 ccctaccaaa ggtgcggttt cgagtgcatt attaagtaat aacaatgaaa aattattacg
      721 cgcaagaacg ataaagaccc atcaggggaa agtttccatt tttctgtccg aagataaagt
      781 ggacaaagat gcctatgtta aattacctgc cccaccgcaa aatttagcca atttgttaaa
      841 gtcaataaat tttccacaac atactaatgc actgggtttt tctgacattg aaataacttt
      901 agaaagttta aaagctatac gatcgcagaa aagaaaacac gtgatcggcg gcagtagtga
      961 acgtcaaccg acgaatgcgc aagtagtcca ttataatagt gccaactcta ttgcgaaatt
     1021 attaggactc gaaggtaatg attggcaatg gtggcattta gtaggccatg ctttacaagg
     1081 ggatgaagcg caaaatattg agaattttgt gttgggaaaa ttgcacgaaa attcaaaaat
     1141 gaatgatttg gctgaagact tagttgaaaa aattttagtt aaattaaaaa aattgggaca
     1201 aaaccccaaa ctgtatttat ccgtcacgcc agaatgggtt ccaggatttg aaatggcacg
     1261 attagctaag aaagttacct ggactttaaa agattcgcct acacaaaatc ccaaacattg
     1321 cgccatattt gagtttgatc cgctttccac cgatgtttta tcgaaaagtg aacaggaatc
     1381 cgtagaaaaa gtattaatgc atatattctt agcagatata cctgtagagg tcgtgtctcc
     1441 agggaaaaaa cctaaatcaa ccctcctatt aagcccagct aaaaatccga aaagttttga
     1501 tatgcaacca accaatgatt ttcctaagaa aaatttactg gcgttgtttg aaaaagaaca
     1561 aattaacctc aactcgagct tggtacaatc aaatcaaaca aagaaaaaca aaatacgtta
     1621 actatgtaaa gattttagta aactttccag taaattagca atttttttct tcctttgcga
     1681 tcataattat ctataatgaa ttatgctgtg aaaattctta taggatattt atggattttc
     1741 atccgaacat tttgtccgcg attggtaata ctcctcttat taaactaaat aaagttgtcg
     1801 ataaaaaatc tgctaccgta ctcgtaaaat gtgaatttat gaacccaact ggctctatta
     1861 aagatcgcat ggcctcttac attatcgaag atgcggaaaa aaagggatta ttaaaacccg
     1921 atggtacaat tgtggaaaat acttctggca atacgggatt gtcgctcgct atggtggcag
     1981 cagttaaagg atatcgctgc atatttacta tgcccgataa aatgagcata gaaaaaatca
     2041 atatgatgaa agcctttggt gcgcaagtca ttgtaacacc cacggatgtg cctggtgatt
     2101 cacccgacca ttatgtaaat acggctcggc gcattgcaca agaaacacat aatggttttt
     2161 atgtgaatca atatcataat cgtgcaaata tcgatgcaca ttactactct acgggtccag
     2221 agatttggag acaaacccag ggaaaattgg attattttgt cgcagggaca ggaacgggtg
     2281 gcacaatatc gggtgtggga a
//
LOCUS       DASTIB010000224         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_987805, whole genome shotgun sequence.
ACCESSION   DASTIB010000224 DASTIB010000000
VERSION     DASTIB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987805"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFP93_03910"
     CDS             <1..1244
                     /locus_tag="VFP93_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843784.1"
                     /translation="TLFKFQDVDRIMKMLQEGKDLKIFDDLFNSPQELIKKGESIRDI
                     TVLIFKNAFKHKEKLMISPEHLHPNDRTYTHATIANMHPSLKNAFKNIFIQLLEKMPL
                     GSTVLKLENEKKIYQKIIAFLNKQDRLFLTHLDRLDVAFQLIRMFRNPECLDQGYTHL
                     CGVATFLQFLIEHTPLSCLEAMMDYCETGYTKNPNLIKYSGKFADDSLVFQSFIQAFK
                     HTNSFYGYYPSYEPMEAITGATKSKSIAEWMEKAGFSDLEDTWALKNPQGQELPLLYR
                     GILGGMYSKYHRQRTHEEQLSALLEAHEQGKAIALNISPLLANRKLFLSTEEEAYRKE
                     MKTKSILGLVYSHWVNLKDIQFHAEEQLVHFKYYTYGKIYSGALPVQYFLKEIYGMVA
                     GTPPTQLEPFIECSTSLSPPL"
     gene            complement(1269..>2250)
                     /locus_tag="VFP93_03915"
     CDS             complement(1269..>2250)
                     /locus_tag="VFP93_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843785.1"
                     /translation="NIAGFNLNATYNRKQNTLFCKIVKLAKARPDVLIDILSTIELEN
                     IDFNEIQKNKYNDTVLSSIFKIAVEMQTPAVIDTLLHFAPFRKLDFVTAKKYAFSLQS
                     KNIIDWLSTTKYADSIPKIFEAGIRQGRKIWEALVKKLDDIEVYNRNFIIFKDHFIKL
                     EDFAHCAQHQQCNTLLSEFYQYILTETNNENPNLSALRNLVQNFSEAIKEKKNDLQLL
                     NDFCNTIACENSYFRKEIALTFAYRCNRERSSTCEYDEPLESLINIKTPVSTDDKKFL
                     LDALIAMREIEDLKSLPCSTKKMVRIKEEKIQDNLVILNNIEKTMASISL"
BASE COUNT          724 a          375 c          393 g          758 t
ORIGIN      
        1 caacattgtt taagtttcaa gatgttgatc gcatcatgaa aatgttacaa gaaggtaaag
       61 atttgaagat atttgatgat cttttcaact ccccccagga attaattaaa aaaggcgaaa
      121 gtatcaggga cataactgtg cttattttta aaaatgcatt taaacacaaa gaaaaattaa
      181 tgattagccc agagcatttg cacccgaatg atcgtaccta tactcatgcc actatcgcca
      241 acatgcatcc ttccttaaaa aatgctttta agaatatatt catacaatta ttagagaaaa
      301 tgcctttggg ttcaaccgtg ttgaaactag aaaatgagaa gaaaatttat caaaaaatta
      361 ttgctttttt gaataaacaa gatcgtctct ttttgaccca tttagatcga ttagatgtag
      421 cttttcagct catacgcatg tttagaaatc cagaatgctt agatcagggt tatactcatt
      481 tatgtggcgt agcaacattt ttgcaatttt taatagaaca tacgccgcta tcatgtttag
      541 aggccatgat ggattattgc gagacaggtt acacgaaaaa tccaaactta ataaaatatt
      601 ccggaaaatt tgcggatgat agtttagttt ttcagtcgtt tatacaggct tttaaacata
      661 ccaatagttt ttacggctat tatccttcct acgaacctat ggaagctatc acaggagcta
      721 ctaaatctaa atctattgcc gaatggatgg aaaaagcagg ttttagtgat ttagaagata
      781 cgtgggcttt gaaaaatcct caaggtcaag agttgcccct attatatcgt ggaattttag
      841 gcggtatgta ttctaaatac cacagacagc gcacgcacga agaacagctc agcgcactcc
      901 tagaagcaca tgaacaagga aaagccatag cacttaacat aagtccatta ttggcaaatc
      961 gtaagttatt tttgtcaacg gaagaagaag cttatcgtaa ggagatgaaa acaaaaagta
     1021 ttttgggtct ggtttatagc cattgggtta atttaaaaga tattcaattc catgcagaag
     1081 aacaattggt tcactttaaa tattatacct acggaaaaat ttattccggc gctttgccag
     1141 ttcaatattt cttgaaagaa atttatggca tggtagcagg aactccgcct acgcaattag
     1201 agcctttcat tgagtgttcg acttctttaa gcccacctct ttagaacgtg ggcttttatc
     1261 agcctctatt ataaggaaat cgaggccatc gttttttcga tattgttcag gataactaga
     1321 ttatcttgta ttttttcttc ctttattcgt accatttttt tagtggaaca cggcagggat
     1381 tttaaatctt caatttctcg catagctatt aacgcatcta aaagaaattt tttatcatcc
     1441 gtggatacgg gagttttgat attaattaag ctttctaatg gttcatcata ttcgcatgta
     1501 gaggatcgct ctcggttaca acgatacgca aaggttaaag cgatttcttt gcgaaagtac
     1561 gaattttcac atgcaatagt gttacagaaa tcgtttaaaa gctgcaagtc gtttttcttt
     1621 tctttaatcg cttctgaaaa attctgcact aaatttctta acgcgcttaa attagggttt
     1681 tcattgtttg tttctgtaag aatatattga taaaattctg atagtaaggt attacattgt
     1741 tgatgttgcg cgcagtgtgc aaaatcttct aattttataa agtgatcttt gaaaattata
     1801 aagttacgat tataaacttc aatatcatcc aattttttta ccaaagcttc ccaaattttt
     1861 ctgccttgac gaatacctgc ctcaaaaatt tttgggatac tatctgcgta ttttgtggta
     1921 ctaagccaat caatgatatt ttttgattgc agtgagaatg catatttttt cgccgtaaca
     1981 aaatctaact tacgaaatgg tgcaaagtgc aataaagtat cgattacagc cggggtttgc
     2041 atttctactg caatcttaaa aatagaagat agaaccgtgt cattatattt gtttttttgg
     2101 atttcattaa aatcaatgtt ttccagttca atggtagata aaatatcgat taaaacatcc
     2161 gggcgagcct ttgctaactt aacaatcttg caaaataatg tgttttgttt acgattgtaa
     2221 gttgcattga gattaaagcc tgcaatattt
//
LOCUS       DASTIB010000225         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_919149, whole genome shotgun sequence.
ACCESSION   DASTIB010000225 DASTIB010000000
VERSION     DASTIB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919149"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFP93_03920"
     CDS             <1..752
                     /locus_tag="VFP93_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843786.1"
                     /translation="NYVHCCHPLNVFQDAIALNYDVKNKKKLIMIRNIKVIESTNHHE
                     IILALEDLSIKGAHFTNGTYAYAAALIVPTVLAGVKRVIQTQMHLPIIIAVNSDESMC
                     RLGKQNFESQSIRSQKVAEPLAKAFPNNQIVVVYYDETTPTELYKVLSAKNFTRTLHK
                     WGYGTDPKQPKIEGAEYFEMVYAFPLPNDIKPICYYDTHIASEPQKIIVDDLRDNLIS
                     REGLILFDLPEELSQYQAKEKSTRKLSYSDS"
     gene            1328..1927
                     /locus_tag="VFP93_03925"
     CDS             1328..1927
                     /locus_tag="VFP93_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9843787.1"
                     /translation="MKKAIKLFLPTAQAIQRLLHPYAEVVIHDIKQNKIVAIYNPFSQ
                     RQVGDSSLLTPEEEMAIEEDYIGPYEKSNWDGKKLKSVSSIIRDENNKAIGMMCINLD
                     VTKLENYQKIIAEFITFNLAKPVPLFKDDWRERINQYVQNYLMEHHLTLETLRREEKK
                     KLIEHLHKVGAFGGKNSANYIAQIIKMSRATVYNYLSDS"
     gene            1943..>2271
                     /locus_tag="VFP93_03930"
     CDS             1943..>2271
                     /locus_tag="VFP93_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015849170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9843788.1"
                     /translation="MLDLKQEVLDASNKIHPYIRETPLEFSINLSKITQAHVYLKCEN
                     FQHTGSFKVRGAFNKLLSLSSIERKQGIVAASSGNHGAAIAFGCNKLNIPGIVFVPEN
                     TSVAKIE"
BASE COUNT          803 a          388 c          394 g          686 t
ORIGIN      
        1 ttaactacgt tcattgttgc catcctttaa atgtattcca agatgccatt gcattaaatt
       61 atgatgttaa aaataagaag aaattaatta tgataagaaa tataaaagtc attgagtcaa
      121 caaatcatca tgaaatcatc ctagcattgg aagatttatc cattaaaggt gcgcatttta
      181 ctaatggaac ctatgcttat gctgctgcgt taattgtacc tactgtttta gctggggtta
      241 aacgggtgat ccaaacgcaa atgcatttgc ccataatcat tgcggtgaat tcagacgaaa
      301 gtatgtgtag gcttggcaag caaaacttcg aatcacaatc aataagaagc caaaaagtag
      361 cagagccact tgcaaaggct tttcccaata accaaattgt agtcgtatat tacgatgaaa
      421 caacaccaac cgaactttac aaagttttaa gcgctaaaaa ttttactaga actttacaca
      481 aatggggata tggaactgat cccaagcagc ctaaaattga aggtgcagaa tattttgaaa
      541 tggtttatgc attcccatta cctaacgata taaagcctat ttgttattat gatacacata
      601 ttgcaagcga gccgcagaaa attatcgtgg atgatttgag agacaattta atttctaggg
      661 aaggactaat attatttgat ttacctgaag aattaagtca atatcaggct aaagaaaaat
      721 caacgcgaaa gctttcttat tccgactcat aatacttcaa tttaagaatt tttgaagcaa
      781 attccaactt ggtgggttca tttctgaaca tgaaagaggt cgataatctg tatcacctct
      841 gctaccttat aggattgttt tctggcgcat acattagctc gcctctgctc cttctggctt
      901 attcaggttt gggtgggcca gttagtgggg ctattataga tttatttaaa gaaccaactg
      961 aaagttgtgc cacacaaaat tcaaacttct cgatgtcaga tacgattcga cgaaacctga
     1021 tagttggaat gaaataagaa gaaacaaacc tgtgcattgg gcttttaaat cgaccaaaga
     1081 agaacacaag attccttctg aacctttacc tcatcaaaaa tcctacacat tataatcgaa
     1141 taattaaagc cggtcctttt ctaggtcgac acgcgctcaa tatacatacc acatgagttc
     1201 actttatata ttcttaaatt tttaaaactt aacaaaaagg tgtaatagca ctacgtaaaa
     1261 ctataaccca ttgacatgtt tgtctaattt agattaaaat gtcaaacatg acattcttct
     1321 aacgtgtatg aaaaaagcca taaaattatt cctacctacc gcacaggcaa ttcaaagatt
     1381 attgcaccct tatgcggaag ttgtaataca tgacattaaa caaaataaaa ttgttgcaat
     1441 ttataatcca ttttctcagc gccaagtggg tgattcttct ttgttaacgc cagaagaaga
     1501 aatggcaatt gaagaagatt atatcggtcc ttatgaaaaa agtaattggg atggtaaaaa
     1561 gctcaaaagt gtgagtagta tcatccgtga tgaaaataat aaagctattg gaatgatgtg
     1621 cattaatctg gatgttacaa aattagaaaa ttaccagaag ataattgcag agtttattac
     1681 ttttaattta gccaaacctg tcccattgtt taaagatgat tggcgagaac gcattaatca
     1741 atatgttcaa aattatctta tggaacatca tttaacgctt gaaacattgc gcagagaaga
     1801 aaaaaagaaa ctaattgagc atcttcataa agtaggtgct tttggtggta aaaattctgc
     1861 aaattacata gcgcaaataa taaaaatgtc tagagcaacg gtttataatt atttgtcaga
     1921 ctcctaagct gaggaaaaac gcatgttaga tttaaagcaa gaagtgctgg atgcgtccaa
     1981 taaaatacat ccttatatac gtgaaactcc gttagaattt tccataaatt taagtaaaat
     2041 tacccaggca cacgtttatt taaaatgtga aaattttcaa cacacgggtt cttttaaagt
     2101 acgcggagct tttaataaat tactttcttt atcatccatc gagcgcaagc aaggtatcgt
     2161 agcggcatcc tcgggtaatc acggcgcagc tattgcattt ggttgtaata aattaaacat
     2221 tcctggaatt gttttcgttc ctgaaaatac ttcggttgca aaaattgaaa a
//
LOCUS       DASTIB010000226         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_634067, whole genome shotgun sequence.
ACCESSION   DASTIB010000226 DASTIB010000000
VERSION     DASTIB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634067"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VFP93_03935"
     CDS             complement(<1..1141)
                     /locus_tag="VFP93_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9843789.1"
                     /translation="MQDTFQCRDQMTVNKKTYTYYSLAKLEKTYPLTRLPFCLKILLE
                     NLLRYEDGINVTRDDIEALLNWDPKQQPSQEIAFTPSRVILQDFTGVPAIVDLAAMRE
                     AMAMLNGNPKKINPLSPVELVIDHSVMVDYFGNNLAFRRNTHREFERNLERYAFLRWG
                     QEAFENFAVVPPGTGIVHQVNLEYLARVIFEKQIGDDLLAYPDTLVGTDSHTTMINGL
                     GVLGWGVGGIEAEAAMLGQPITMLIPQVIGFEITGSLPEGSTATDLVLVVCEMLRKHG
                     VVGKFVEFFGEGLNHLPLADRATIANMAPEYGATCGIFPIDQETLNYLALTGRVSEKI
                     AFIEAYCKNQHLFRDDTRRAEYSDIIQLDLSSVEPCIAGPKRPQDR"
     gene            1308..1757
                     /locus_tag="VFP93_03940"
     CDS             1308..1757
                     /locus_tag="VFP93_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9843790.1"
                     /translation="MEANEPIDYRRINAKCACFNLRKATRAVTQYYDHCLEPAGIRVT
                     QFTLLASLASVSARTLTEMANSLVMDRTTLTRNLKPLEKLGLIHTIEARDRRSKAYAL
                     TELGKETLNKGVPLWHEAQQKLIQGLGEEQYEKILVEMDSVTKVVGE"
     gene            1776..2801
                     /gene="rpoS"
                     /locus_tag="VFP93_03945"
     CDS             1776..2801
                     /gene="rpoS"
                     /locus_tag="VFP93_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018692345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HET9843791.1"
                     /translation="MTGWESVVENSELEPEVDEEIPEVVESLPSLALSDEQEMEFEWN
                     TMPSSFNEIEEAIRESSRKSLDVTQIYLGAIGFAPLLSAEEEIYYARKVLQGDAIAKK
                     RMVESNLRLVVKIARRYLNRGLSMLDLIEEGNLGLIRAVEKFDPEKGFRFSTYGTWWI
                     RQTIERALMNQTRTIRLPIHIVKEMNVYLRISRELAQKLDHEPSFEEIAIVAKKSAAE
                     VEKILSLNERVTSLETPVGGETEKYLMDVVGEDEFSNPLTELEDADFKRELEAWLDQL
                     SEKQTAVVVRRFGLRGFEQCTLEDVGKDIGLTRERVRQIQVEALEKLKEILKTRGLTP
                     EILFASF"
     gene            complement(2798..>4376)
                     /locus_tag="VFP93_03950"
     CDS             complement(2798..>4376)
                     /locus_tag="VFP93_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9843792.1"
                     /translation="HIQVEYKKDHWTIHANPVHWKHPQYGVLPEIELNFTLYDQKKYT
                     GTVEVLFSKNKIAGTYTIDPNQDHALEGLLKGDFNDISFLSKFSKDLKKLNGHIQATL
                     HLGGHLKKPKFWGESTLTKTQIPLPALGTDIKLQQLHLHSKGDGNLTITGTGQLGTGD
                     FSLNGSGNFLNDTPQMHLALTGKNLLVSKTPEYSIWANPNVTIQIKNGTPEITGSIFI
                     PKAKITMQSKQNYIGTSSDVIIVKNKQSLTPVEKPHPLQQFKTHITIELGEDVHFKGY
                     GLDSYFVGKVNVSGEQGKPWLGSGKLNLKEGKFKARNRVLHLTKGQIFYAETPLINPA
                     LDIRAQREVKPIVQHTTTTTRDLFAPYEMPVEKIIVGVALTGTLKSPHIRLYSQPSLP
                     EGDILSYLILDKPQKAASFTEGKILFEAASEMMHQFGLQKNDIRPSLVEKFHLDTLDL
                     KTANTRDPSKSALEDTVLVVGKQISDRLYAEYSVGLMDKASTLSLRYLVGKHITLETQ
                     ASKNSVSADILFIFESG"
BASE COUNT         1327 a          833 c          889 g         1327 t
ORIGIN      
        1 cgcgatcttg tggacgcttt ggtcctgcaa tacaaggttc aacgctagat aaatctaatt
       61 ggatgatgtc actatattct gcacgtcggg tatcgtcgcg aaataaatgt tgatttttac
      121 aatacgcttc gataaaagct attttttcgc tcacgcggcc cgtaagagct aagtaattta
      181 acgtctcttg atcaatgggg aaaataccac atgtcgcccc atattctggc gccatattag
      241 caatagttgc tctatctgcc aaaggcaaat ggtttaatcc ttcaccaaaa aattccacaa
      301 attttccaac aaccccatgc tttctcagca tttcacaaac aaccaatacg agatcggtag
      361 ccgtggaacc ttctggaaga ctaccggtta tctcaaatcc aatgacttgc ggaattaaca
      421 tggttatggg ttgccccagc atggccgctt ccgcttcaat cccgccaaca ccccaaccta
      481 aaacacctaa accgttgatc atcgttgtat gagaatcggt acccaccaaa gtatctggat
      541 atgccaatag atcatccccg atttgctttt caaatataac gcgcgctaaa tattctaaat
      601 tgacttgatg tacaattccg gtgccagggg gaactaccgc aaaattttca aaggcttctt
      661 gaccccaacg taaaaatgca tatctttcta ggtttctttc aaattcacga tgggtatttc
      721 tacgaaatgc aagattattg ccgaaataat ctaccatgac ggagtgatcg atgactaatt
      781 ccacaggcga aagaggatta attttttttg gattgccatt aagcattgcc atggcttctc
      841 gcatggcagc taaatccaca atagcaggaa cacctgtaaa atcttgcaag ataacgcggc
      901 taggtgtaaa agcaatttct tgactgggtt gttgtttagg atcccaattt aataatgcct
      961 caatatcgtc tcgcgtcaca ttaatgccgt cttcgtaacg caataaattt tctaataaga
     1021 ttttcaaaca aaaaggtaaa cgtgtgagtg gataggtttt ttctagtttg gctagcgaat
     1081 agtaagtata tgttttttta ttcactgtca tttgatcgcg acattggaat gtatcctgca
     1141 tcggttctcc tttttcttaa cctaaccctt ttgcctgcgc ccagtgtatg actttttcgt
     1201 taaaattgcg agttgtacca aaggcttaaa aattatttgt gaatctaaac taaaaactct
     1261 tgacagcttc ctacgagatc ctacactttt tgtgaggagg gtaactcatg gaagctaacg
     1321 aaccaattga ttatcggcgg attaatgcca aatgtgcctg ctttaattta cgtaaagcaa
     1381 cgcgtgctgt aacgcaatat tatgatcatt gcctagaacc tgcgggtatt agggtcaccc
     1441 aatttacctt attggcatct ttggcgtcag tttctgcacg aaccttaacc gagatggcaa
     1501 attctttggt tatggataga acgacactca cgcgcaattt aaaaccttta gagaagctag
     1561 gcctgattca tactattgaa gccagagatc gacgttctaa agcgtacgct ttaacggaac
     1621 tgggcaaaga aactttgaat aaaggtgtac cattatggca tgaggcgcaa caaaaattaa
     1681 ttcaaggatt aggggaagag cagtacgaaa aaattcttgt cgaaatggac tcggtcacca
     1741 aggtggtggg tgaataataa aaaaggtgca aaactatgac tggttgggaa agtgtagttg
     1801 aaaatagcga gcttgaacca gaagtggacg aagaaatccc agaggttgtt gaatcgcttc
     1861 cgagcttggc attatcggat gaacaagaaa tggaattcga atggaatacc atgccttcat
     1921 cttttaatga aattgaagaa gcgatacgcg aatcaagcag aaaatccttg gatgttaccc
     1981 aaatatattt aggggctatt ggatttgctc ctcttttatc tgcggaagaa gaaatttatt
     2041 atgcgcgcaa agttttacaa ggcgacgcaa tagcaaaaaa aagaatggta gaaagtaatt
     2101 tacgcctagt ggtaaaaata gctagacgat atttaaatcg aggattatcc atgctcgatc
     2161 ttatcgaaga aggaaattta gggttaattc gtgcggtgga aaaatttgat ccagaaaaag
     2221 gatttagatt ttcgacctat ggcacttggt ggattcgcca aacgattgaa cgcgccttaa
     2281 tgaatcaaac acgcaccata cgcttaccga ttcatattgt aaaggagatg aatgtgtatt
     2341 tgcgtattag ccgtgaatta gcacaaaaac ttgatcatga gcccagtttt gaagaaatcg
     2401 caattgtggc caaaaaatct gcagcagaag tcgaaaaaat tttaagttta aatgaacgtg
     2461 ttacctcgtt agaaaccccc gtcggtggag aaacagaaaa atatttaatg gatgttgtag
     2521 gagaagatga attctcaaat cccctcacag aactcgaaga tgcagatttt aaaagagaac
     2581 tggaagcatg gttagatcaa ttaagcgaaa agcaaaccgc cgtcgtagtg cgtcgatttg
     2641 gtttacgtgg ttttgaacaa tgtacattgg aagatgttgg taaagatata ggtttaaccc
     2701 gagaaagagt ccggcaaata caagtagaag ccttagaaaa gttaaaagaa attctcaaga
     2761 ctcgaggatt aacgccagaa attttattcg cgagttttta accactctca aaaataaaca
     2821 aaatatccgc gcttacggaa ttcttagatg cttgcgtttc taaggtaata tgtttcccca
     2881 ctaaataacg caagctcaaa gtgctcgctt tatccatgag gcctaccgaa tactccgcat
     2941 acaagcgatc ggaaatttgt ttacctacca ccagtaccgt gtcttctaat gcggactttg
     3001 aaggatcacg ggtattagcg gtttttaagt ccagggtatc taagtgaaat ttttcgacca
     3061 aacttggacg aatgtcgttt ttttgcagac caaattgatg catcatttcg ctagcagctt
     3121 caaataatat ttttccttcg gtgaacgaag ctgctttttg cggtttatct aaaataagat
     3181 acgataaaat atcgccttcg gggagcgaag gttgcgaata gagtcgaata tgcggagatt
     3241 tcagagtgcc ggtaagggct acacctacaa taattttttc cacaggcatt tcgtaagggg
     3301 caaataaatc tcgcgttgtt gttgtcgtgt gctgcactat gggttttact tccctttgtg
     3361 cgcgtatatc taatgctgga ttaataagag gcgtttcggc gtaaaaaatt tgaccttttg
     3421 ttaaatgtag cacgcggttt ctcgctttaa acttaccctc ttttaaatta agttttcctg
     3481 agcctagcca aggtttgccc tgttcgcccg aaacatttac tttgcccaca aaataggaat
     3541 ctaatccata ccccttaaaa tgcacgtctt ctcccaattc tattgttatg tgcgttttaa
     3601 attgttgtaa aggatgtggc ttttctaccg gggttaagct ttgcttattt ttaacaataa
     3661 tgacatcgga agaagtgcca atataatttt gttttgattg catagttatt ttagctttcg
     3721 gaataaaaat actaccagtg atctcaggtg taccattttt gatctggatg gttacattag
     3781 gattagccca tatcgaatac tctggagttt tgcttaccaa aagattttta ccggttagcg
     3841 ctaaatgcat ttgtggagtg tcgtttaaaa aattaccaga accgttcagc gaaaaatcac
     3901 ctgttcctaa ttgaccggtt ccggtaatag taagattacc atcacccttg ctatgtaaat
     3961 gtaattgctg gagtttaatg tctgtgccca aggcgggaag aggaatttgc gtttttgtta
     4021 atgtactttc accccaaaat tttggttttt ttaggtgtcc accaagatgc aaagtggctt
     4081 gtatatgacc atttaatttt tttaaatctt tagaaaattt ggataaaaaa cttatatcat
     4141 taaaatcgcc ctttagtaat ccttctagcg catgatcctg attcggatca attgtgtaag
     4201 taccagcgat tttatttttt gaaaataaga cttctaccgt acctgtgtat tttttttgat
     4261 catataaggt gaaattgagt tctatttcag gtagaacgcc atattgagga tgtttccaat
     4321 ggacaggatt ggcatgaatc gtccaatgat cctttttata ttctacttga atatgc
//
LOCUS       DASTIB010000227         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1315201, whole genome shotgun sequence.
ACCESSION   DASTIB010000227 DASTIB010000000
VERSION     DASTIB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315201"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFP93_03955"
     CDS             <1..1195
                     /locus_tag="VFP93_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9843793.1"
                     /translation="IKALKNAESANNAKTEFLANMSHEIRTPMNGIMGMLQLLQHTHL
                     TEKQISFVELSLASANSLLKILDDILDYAKIVSGEISLESIKFNIVDEIERMLNIFKI
                     QCDKKNIAIELNISDQIPATLIGDPLRLGQIIMNLVNNAIKFTPQGGKISISLSPQEM
                     HDNYIIMHCSVKDTGIGIPLEMQSKIFQKFSQADESITRIYGGTGLGLAICKQLVEIM
                     GGTIYVISKENEGSNFIFTFKIHYLSTSLENMSEEKQVLKNSLLPYAIQVLVVDDIES
                     NRILAREILTMLGCHVDMVESGELALRAIEYKDYHLILMDCQMPGLDGFKTTEFIRKI
                     ETHNHKRHVIIALTANALEQTRNRCLDAGMDDYLTKPIELDLLLIALKKNLHLSSVPE
                     IKEAK"
     gene            complement(1225..2130)
                     /locus_tag="VFP93_03960"
     CDS             complement(1225..2130)
                     /locus_tag="VFP93_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9843794.1"
                     /translation="MQRKILLVDDEKMNLEIMEGYLRLGENADHTIIKASNGQEGLEF
                     LKSQANEIDAILLDRMMPHFSGIDFLKERNKDEELKNIPVIMQTASTSKENMLESFKH
                     GAYHYLIKPYSPTIFNSIVRSAINFYSKYRDLTSVLQNSKTLFSYVNEAEFRIVTPED
                     ADLLSLSLARLFPNPDRVVFGIFEISLNAIEHGNLSITYDEKTELNISGKWREEVHRR
                     LKLEENVGKEVILRYFKKNGEIVLNIKDQGEGFDFKKYLDFDLSRSTDNHGRGIAFAK
                     ALSFDRLEYVEPGNEVNCIVKQLKF"
     gene            2302..>3579
                     /locus_tag="VFP93_03965"
     CDS             2302..>3579
                     /locus_tag="VFP93_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HET9843795.1"
                     /translation="MMEWYHQFFQTGPIPSNVMLGEYNYFLVFVSYLIASFASFVALD
                     MSAHLRRPASSLFRVCWLVGGAFAMGAGIWSMHFVGMLAFIMPMPMGYDLFWTGLSMV
                     AAIIAAALAFMFFIIPNPQPRHYILSGVILGSAIPTMHYLGMAGMRGVEIHYIPLYFY
                     CSILIAVIAATVGIWLSLKSDRRDFKTQFSWKLISALIMGFAITAMHYMGMYAAVFIP
                     DHSEHHMTTPVDQVLLALFITIIVCSILIVALILSTARYLISVTEKNKNFLKIVLDNM
                     REGVIACDASGKITLVNRAAKTLFEDFAPITSILSDWLLQFDYLNAKTDRIDTPSQFP
                     LNRALQGETVRDCEIIVKGAAQKKTILLIDGQPLVGMNNENLGAVIVWNDISSSTAAE
                     QANKLKTAFLANMSHEIRTPLNGVMGMLQLLMTT"
BASE COUNT         1116 a          655 c          641 g         1167 t
ORIGIN      
        1 aataaaagca ttaaaaaatg ctgaaagtgc aaataatgca aaaaccgaat ttctcgccaa
       61 tatgagtcac gaaatccgaa cacccatgaa tggtattatg ggaatgttac aattacttca
      121 acacacccat ttgacggaaa aacaaatttc ttttgtagaa ttaagtcttg cttccgccaa
      181 ctcactactc aaaatattag acgatatctt agactatgcc aaaatagttt ctggcgaaat
      241 ttcactggaa tctattaaat ttaacattgt cgatgaaata gaaagaatgt taaacatatt
      301 taagattcaa tgtgataaaa agaatatagc gatagaatta aatatttcgg atcaaattcc
      361 tgccacgctt atcggtgatc cactaagact tgggcaaata ataatgaatt tagtcaacaa
      421 tgcaattaaa tttactccgc aaggtggaaa aatttccatt tctttatcac cacaggaaat
      481 gcatgataat tatataatca tgcattgctc ggtaaaagat actggtattg gaattcctct
      541 tgagatgcaa tcaaaaatat ttcaaaaatt ttcgcaagcg gatgaatcta tcacgagaat
      601 atatggtgga accggccttg gccttgccat ttgcaaacaa ttggttgaga taatgggagg
      661 taccatttat gttatttcaa aggaaaatga aggttctaat tttatcttta cgtttaaaat
      721 ccattatctg agcacatccc ttgaaaatat gtccgaagaa aagcaggttt tgaaaaatag
      781 tttactgcct tatgctattc aagttttagt agtcgatgat attgaatcga atcgaatttt
      841 agcgcgtgaa atactaacca tgctgggttg tcatgtagat atggtagaat ctggagaact
      901 tgcgcttcgg gcaattgaat ataaagatta ccatctaatt ttaatggatt gtcaaatgcc
      961 ggggttggat ggctttaaaa caacggaatt cattagaaaa attgaaacgc ataatcacaa
     1021 aaggcatgtc attattgcct taactgccaa tgcattagaa caaacacgca accgctgtct
     1081 tgacgcagga atggatgatt atttaactaa accgattgaa ttagatctgc ttctcatcgc
     1141 acttaagaaa aatttgcatt tatcctctgt accagaaata aaagaagcaa aatagtaaaa
     1201 tattattttt ttatttagga atatttaaaa ttttaattgt ttaactatac aattaacttc
     1261 attgcctggt tctacatatt caagacgatc aaaactaagc gctttggcaa aggcgattcc
     1321 acgcccatgg ttgtctgtac ttcgcgataa atcgaaatca agatatttct taaaatcaaa
     1381 cccctccccc tgatccttga tatttaatac aatctcgccg ttttttttga aataacgaag
     1441 tatcacttct tttccaacat tttcttctaa tttcaaacga cgatgcactt cttcccgcca
     1501 tttcccagat atattcaact cagttttttc gtcgtaggta atagagagat ttccatgttc
     1561 aatcgcattt aaggatattt caaatattcc aaagaccacg cgatccggat ttggaaacaa
     1621 acgcgccaag cttaagctta agagatccgc atcttctgga gtgacaattc gaaattccgc
     1681 ttcgttcaca taagaaaata atgtttttga attctgtaag accgaggtga gatcacgata
     1741 tttggagtaa aaattgattg cagagcgtac aattgaatta aatatggtcg gtgaataggg
     1801 tttgatcaaa tagtgatacg ccccatgttt aaaactttca agcatatttt ctttacttgt
     1861 ggaagccgtt tgcatgataa caggtatatt ttttaattct tcatctttat tccgttcctt
     1921 aagaaaatct atacctgaaa aatggggcat catccgatct agtaaaatag catctatctc
     1981 gtttgcctga gatttaagga attctagtcc ttcctgtccg ttactcgctt ttataatagt
     2041 atgatcagca ttttctccta accgaaggta accttccatt atttcgagat tcattttttc
     2101 atcatctact aaaagaattt ttctttgcac tttatcctct acactggttt ttaagacaat
     2161 gataattttt ctttattaaa ataattttag tcaaaatact agcgataccg cttaaaaatt
     2221 acacttaata gacgattttt tttactatga gaatagaatt tgcctaatct ataaataaga
     2281 gctgtgaaag aagttaacta catgatggaa tggtatcatc agttttttca gacaggacct
     2341 atacccagta atgtaatgtt aggtgaatat aattactttc tggtatttgt gtcctattta
     2401 atagccagct ttgcctcttt tgttgcccta gatatgagcg cgcatctaag aagaccagct
     2461 tcttctttat tccgtgtatg ttggctggtt ggtggtgcat ttgctatggg tgccggtata
     2521 tggtctatgc attttgtggg catgttagcg tttatcatgc caatgcctat ggggtacgat
     2581 ctcttttgga caggtttgtc tatggtcgct gcaatcatcg cagcagcact tgcattcatg
     2641 ttcttcataa tcccaaaccc tcaacctcgg cattatattc taagtggcgt gattctaggc
     2701 tctgctatac ccaccatgca ctatttagga atggctggta tgcggggcgt tgaaattcat
     2761 tacatacccc tatattttta ttgctcgatc ttaatagccg tcattgctgc cactgttggg
     2821 atttggctat cactcaaaag tgatcggcgc gattttaaaa cgcagttttc ttggaagctc
     2881 attagcgcgc tcatcatggg atttgcgata acggctatgc attatatggg aatgtatgct
     2941 gctgttttta tacccgatca cagtgaacat catatgacta cccctgtcga tcaggtactt
     3001 ttagcattat ttattaccat aatagtttgc agtattctca ttgtcgcact tattctttcc
     3061 actgcgcgct atttaatatc ggttacagaa aaaaataaaa attttcttaa aattgtgcta
     3121 gataatatgc gcgagggtgt aatagcctgt gacgcatccg gcaaaattac tttggtaaat
     3181 cgggctgcga aaaccttatt tgaggatttt gcgcctatta cttctattct ttctgattgg
     3241 ttattgcaat tcgattactt aaacgcaaaa accgatagga tcgatacacc cagtcaattt
     3301 cctttaaatc gcgcactgca aggcgagacg gtaagagatt gcgaaattat agtgaaaggt
     3361 gcagcacaaa aaaaaactat attgctgatt gacggtcagc ctctcgtcgg tatgaataat
     3421 gaaaatttag gtgcagttat tgtatggaat gatattagtt ccagcactgc tgctgagcag
     3481 gctaataagc ttaaaacagc ttttcttgct aacatgagcc atgaaataag aacaccactc
     3541 aacggggtta tgggaatgtt acaactgctg atgacaaca
//
LOCUS       DASTIB010000228         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_139681, whole genome shotgun sequence.
ACCESSION   DASTIB010000228 DASTIB010000000
VERSION     DASTIB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139681"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..455)
                     /locus_tag="VFP93_03970"
     tRNA            complement(365..455)
                     /locus_tag="VFP93_03970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(419..421),aa:Leu,seq:taa)
     gene            complement(473..546)
                     /locus_tag="VFP93_03975"
     tRNA            complement(473..546)
                     /locus_tag="VFP93_03975"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Cys,seq:gca)
     gene            complement(571..646)
                     /locus_tag="VFP93_03980"
     tRNA            complement(571..646)
                     /locus_tag="VFP93_03980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(611..613),aa:Gly,seq:gcc)
     gene            complement(721..1269)
                     /gene="pgsA"
                     /locus_tag="VFP93_03985"
     CDS             complement(721..1269)
                     /gene="pgsA"
                     /locus_tag="VFP93_03985"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017444621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9843796.1"
                     /translation="MLSIPNMFTFSRIALIPFFVLVFYVPGPYSNYAAAIIFLIAALT
                     DWIDGFLARKLQQTSKFGAFLDPVADKLLVACALVLIAVEFRNFWITIPAIVIVCREI
                     AVSALREWMAEVGQRTRVKVSVWGKIKTVFQLVAVIILISQPPDWNIPLVWLGVFLMY
                     SAVLLTIWSMVEYLRAALVTLR"
     gene            complement(1340..>2132)
                     /locus_tag="VFP93_03990"
     CDS             complement(1340..>2132)
                     /locus_tag="VFP93_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9843797.1"
                     /translation="QKKKWQKLAHENAKLALKQRIGSRKVFFKRLQSLKEVLGVQELQ
                     HIECFDISHSGGEATVASCVVFDENGPNKSLYRQYNIQEGGSDDYKAMREVLIRRYKK
                     AKEENAKLPDLIVIDGGKGQLSTALSALKECQIVDVLVMGVAKGENRKPGLETLFIAT
                     SSNGEEVSVVTLAPTSEALHYIQHIRDEAHRFAITRHRTKISKKRVSSPLENIPGIGP
                     KRRQQLLMHFGGKQGLMGASEEAIAAVPGINKKLARLIYQSLHES"
BASE COUNT          620 a          483 c          414 g          615 t
ORIGIN      
        1 tccattacaa tccaaaatta cttttaacat aaaattcctt tttcataagg cataggaaga
       61 tatacgataa ttaacttgtt ttgtgttgtc taatgttttt aatcttattg tatccatcca
      121 tatcgcaaat tttaaaaaag aaatctgaag tgctcgagat ttgggataga gccaattaag
      181 ctcctttcag gcaggagcct ggattcccgt tttcacggga atgacgccat cagcttagac
      241 tcacgtcttt gagcatagtt tggagattga agtgcccccg gaattgggat agagccgagt
      301 taaagtgctt tgctcatagt ttggaaactg aagtgcccct ggaattggga tagagccaat
      361 ctaatggtgc ccggggccgg aatcgaaccg gcacggagtg ttaaatctcc gagggatttt
      421 aagtcccttg cgtctaccta tttcgccacc cgggcatatt taaaaaataa cttggaggct
      481 gaggccggaa tcgaaccggc gtccacggct ttgcaggccg ctgcatgacc actctgccac
      541 ccagccggtg cgctcagact caaaaagatt tggagcggga aacgagactc gaactcgcga
      601 ccccaacctt ggcaaggttg tgctctacca actgagctat tcccgcgtta caggtgtgca
      661 ttgtaaggat ttccttttcg ctgtcaagca ttgatttttg cctaataact tagaaaataa
      721 ctaacgtaat gtaactaacg cggcacgtaa atattccacc atcgaccaaa ttgtcaataa
      781 tacagcggaa tacattagaa aaacgcctaa ccataccaag gggatattcc aatctggggg
      841 ctgagaaatt aaaataataa cggcaaccaa ctgaaatacg gtttttattt tcccccacac
      901 actaactttt acgcgagtac gttgaccaac ctctgccatc cattctcgta aagcagatac
      961 agcaatttcc ctacatacaa ttacaatggc gggaatcgtg atccaaaaat ttctaaactc
     1021 aaccgcaata agcactaaag cacaagcgac gagaagctta tcagcaaccg gatccaaaaa
     1081 ggcaccgaat tttgaagtct gttgaagttt acgagctaaa aatccatcga tccaatcggt
     1141 aagtgctgca ataagaaaaa taatggcagc tgcataatta ctataaggtc ccggaacata
     1201 aaacactaac acgaagaagg gaatgagtgc tatgcgactg aaggtaaaca tattagggat
     1261 gcttaacatg agggctcagt ccatacattt aacttataag tttataacat gcagaggcgg
     1321 tagtggcaag taaagcttgt caactttcat gcaggctttg ataaataagc cgtgcaagtt
     1381 ttttatttat tccaggtacc gctgctatag cctcttcact tgcccccata agcccctgct
     1441 taccaccaaa atgcattaaa agctgttgac gtcttttggg gccaataccg ggtatatttt
     1501 ctaaaggtga gctcacgcgc tttttcgaaa tcttggttcg atgacgggtg atagcaaacc
     1561 gatgcgcttc gtctcgaata tgttgaatat aatgtaaagc ttcgctggtt ggtgctaatg
     1621 tcacaacact tacttcttct ccattggaac tggtcgcgat aaaaagcgtt tctaaaccag
     1681 gcttgcgatt ttctcctttt gcgacaccca tgaccaaaac atcaacaatt tggcactctt
     1741 ttaacgcaga aagggctgta cttaattgcc cttttccacc atcaattaca ataagatcgg
     1801 gcaacttcgc attttcttct ttggcttttt tataacgacg aattaacacc tctcgcatgg
     1861 ctttataatc gtcgctccca ccctcttgaa tattgtattg tcgatacaaa gatttatttg
     1921 gcccgttttc atcaaatact acgcaggatg caacggttgc ctcccctcca ctatggctta
     1981 tatcaaagca ttctatgtgt tgaagttctt gtactcctaa aacttctttt aaagactgca
     2041 accgtttgaa aaaaactttt ctactaccta ttctttgttt taatgctaat tttgcattct
     2101 catgcgctaa tttctgccac ttttttttct gt
//
LOCUS       DASTIB010000229         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1375499, whole genome shotgun sequence.
ACCESSION   DASTIB010000229 DASTIB010000000
VERSION     DASTIB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375499"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFP93_03995"
     CDS             complement(<1..997)
                     /locus_tag="VFP93_03995"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET9843798.1"
                     /translation="MASSGFSWKSPWTALAHDMLSIPIAWFFAYWVRFNLSTIPSEIL
                     HHAVEMLPWVMGAQACSFWFFGLYRGVWRYASLPDLVRIFKAVSIGCLFSLFLLFFLF
                     RLNHMPRTVFPMYGTLLLLLLGGSRFFYRWLKDFRSSLGIAYQRVLIVGAGTAGESLC
                     RDLLRDQTAYLPVAFVDNNPEDLGREIHGVQVKGNYVKIPDIVQTMAIDLILLAIPNA
                     NAKEIRYIHTLCQSTGKPVHTLPSIDDLFEGRVSVKNLRKISLSDLLGRDPVNLDKKA
                     IQQSLTHKVVLISGGAGSIGSELCRQVAKFQPQELVIIDNNEYHLFLLEQEFKTLN"
     gene            complement(1043..2095)
                     /locus_tag="VFP93_04000"
     CDS             complement(1043..2095)
                     /locus_tag="VFP93_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9843799.1"
                     /translation="MSWGNFCLLLFGLSWALTGIYRSHALSKGYVDEPNARSAHFVIT
                     ARGGGIVFASLWFVLLAVSGYMNWIHPKFLCIFGPAFFVALVGFKDDRKSASMWLRLA
                     VQMLATVASFYFLQLDPFQLITHFSLSESINLALLMLGVLWMTNLMNFMDGSDGLAAT
                     EAIMVLAAGGVLMSWAHGYSLTILLFGLVSLVAGFLTWNMPKAAIFMGDSGSGFLGYV
                     IAVFALVTYKWYKIPFEIWVILTGAFWFDASITLIRRVLAGDKWTQPHCLHAYQRLIH
                     GGWSHSAVLFGLICVNVVLISMAIWAFKEPRFLHMILGMSVCFLTVIYIMVEIYKPMY
                     KTWHNAPSKNMKKVTT"
     gene            complement(2092..2805)
                     /gene="pyrF"
                     /locus_tag="VFP93_04005"
     CDS             complement(2092..2805)
                     /gene="pyrF"
                     /locus_tag="VFP93_04005"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9843800.1"
                     /translation="MMGIHTDPRFIIAFDFNAIEEADTLLQHLDPRLTRIKVGKAMFV
                     KYGPNFVKYLQTKGFEVFLDLKFHDIPNTVYNACKEAAHLNVWMLNVHALGGRFMLEQ
                     AVKAVQSHPNAKKPLLIGVTLLTSLHQDDLTFFNQEGDLNSLTLRLAGLCYEAGLQGV
                     VCSAHEVPMIKQEFGKDFICVTPGVRLDTQEKQDQKRVATPKEAFELGADYIVIGRAL
                     TQSNDPKTVLEKFFPLGAV"
     gene            complement(2875..>3378)
                     /locus_tag="VFP93_04010"
     CDS             complement(2875..>3378)
                     /locus_tag="VFP93_04010"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HET9843801.1"
                     /translation="NIEKSQGKYKQALKAYKQVQYQDPDFVCEVIIPIQQCYEKLNAK
                     EDMLRYLDQVIKSCPHISVVLATAAQYKRFRGDREAAVFLAEHMREKPSIRGIRHLVD
                     YHLVQAKEEVRDDLLILRCMMDKLLENKPTYRCNHCGFSTKTLHWQCPSCRQWSSIKP
                     IHRIDGE"
BASE COUNT         1091 a          727 c          574 g          986 t
ORIGIN      
        1 gattaagcgt tttaaattct tgttctaata aaaacaaatg gtattcattg ttatcaataa
       61 taacgagttc ttgtggttga aatttcgcta cttggcgaca taattcggaa cctatagaac
      121 ctgctccacc actaatgagc acaactttat gggttaagct ttgttgaatg gcctttttgt
      181 caagattaac cggatcacgc cccaataaat cgcttaaaga aatcttgcgt aaatttttta
      241 cggaaacacg tccttcaaaa agatcgtcaa tactaggaag cgtatgtact ggtttacccg
      301 tggattgaca taacgtgtga atatatctaa tttctttggc attagcatta ggaattgcca
      361 gcaaaattaa atcaatagcc atagtttgca caatgtctgg gatttttaca tagtttcctt
      421 taacttgaac accatgaatt tctctcccta aatcttctgg attattatca acaaatgcaa
      481 caggtaggta agcagtttga tcgcgtagca aatctctaca taaactttca ccggcagtac
      541 ccgcgcccac tattaataca cgttgataag caattcctaa ggaagaacgg aaatctttaa
      601 gccagcgata gaaaaagcga gaaccaccca ataacaaaag cagcaaagtc ccatacatgg
      661 gaaaaacggt tcttggcata tgattgagtc tgaacaagaa aaataataaa aaaagcgaaa
      721 ataagcatcc gatacttacc gccttaaaaa tgcgtactaa gtccggtaaa gaagcatagc
      781 gccatacacc tcggtataaa ccaaaaaacc aaaaagaaca ggcttgcgcc cccataaccc
      841 agggtagcat ttcaacagca tggtgcagga tttcggaagg tatagtactt aagttaaaac
      901 gtacccaata cgcaaaaaac cacgcaatgg gtatgcttaa catgtcatgg gctaatgcgg
      961 tccagggact tttccaagaa aagccagagc ttgccatgta ttcctctttg ttattattat
     1021 ttccattttt aaatttttaa tcttacgttg tgactttttt catattttta cttggagcat
     1081 tatgccatgt tttatacatc ggtttataaa tctcgaccat aatatatatc acagtaagaa
     1141 aacatacaga catgcctaaa atcatatgta aaaatcttgg ttctttgaat gcccagatgg
     1201 ccatactaat aagtacaaca tttacgcata ttaagccgaa caataccgca gaatgtgacc
     1261 atccaccatg aattaagcgc tgataagcat gcaaacaatg tggttgagtc catttatcac
     1321 cagctaatac tctacgaatt aaagtgatgc tcgcatcaaa ccaaaatgct cccgttagta
     1381 taacccaaat ctcaaaagga attttatacc atttataagt aactaaggcg aatactgcaa
     1441 taacgtatcc aagaaaacca cttccagaat ctcccataaa aattgctgct ttgggcatat
     1501 tccacgttaa aaaacctgca actagagaaa ctaatccgaa tagtagaatt gtcaatgagt
     1561 agccatgcgc ccaactcatc aaaactccgc ctgcggctaa taccataatc gcttcagtcg
     1621 cagctaaacc gtcacttcca tccataaaat tcataaggtt ggtcatccaa agcacaccta
     1681 acattaaaag tgctaaattg atgctttcag ataaagaaaa atgggtaatt aactgaaaag
     1741 gatcgagctg tagaaaataa aaactcgcca ccgtagcaag catttgcaca gctaatctca
     1801 accacatcga tgcacttttc ctatcgtctt tgaagcctac gagggccaca aaaaatgcgg
     1861 gcccaaaaat acataaaaac ttaggatgga tccaattcat ataaccgctt acagctaaaa
     1921 gaacgaacca gagtgacgcg aaaacaatac cgccccctct tgctgtaatt acgaaatgcg
     1981 ctgatcgtgc attgggttca tcgacatagc ccttacttaa ggcatgtgat cgataaatac
     2041 ccgttaatgc ccagcttaat ccaaacaata aaagacaaaa attaccccaa ctcatactgc
     2101 tcctaaagga aaaaactttt ctaacacggt ttttggatcg ttactttgcg taagtgctcg
     2161 tccaataaca atataatctg ccccaagttc aaatgcttcc ttaggggtgg ctactctttt
     2221 ttgatcttgt ttttcttggg tgtctaagcg cacacccgga gttacgcaaa taaaatcttt
     2281 cccgaactct tgtttaatca taggaacttc gtgtgcagag caaacgaccc cctgtaatcc
     2341 tgcttcataa caaagaccag ccaatcttag cgtgagcgaa tttagatcgc cttcttgatt
     2401 aaaaaatgtt aaatcgtctt gatgcaaact ggtcaacaag gtaacaccta ttaataacgg
     2461 tttttttgca ttaggatgtg actgcacagc ttttaccgct tgttccagca tgaatctacc
     2521 acctaaagca tgcacattta acatccaaac gtttaaatgg gccgcttctt tacaagcgtt
     2581 gtacactgtg ttgggaatat cgtgaaattt taaatccaaa aaaacttcaa atcctttggt
     2641 ttgtaaatat ttcacaaaat ttggcccgta ctttacaaac atggctttac caacttttat
     2701 tcgggtaagt cttgggtcca agtgttgtaa aagcgtatcc gcttcttcta ttgcgttaaa
     2761 atcgaacgcg atgataaatc taggatccgt atgtatgccc atcataattc actctttctt
     2821 tatttaaaat tttatagttt ttcgtaactg ggtgatatgc tggttcatcc actactattc
     2881 gccatcaatc cggtgaatgg gttttataga tgaccattga cgacaactag ggcattgcca
     2941 atggagcgtt ttggttgaaa agccacaatg gttacatcgg tacgttggtt tattttctaa
     3001 taatttatcc atcatacatc gcaaaatcag taaatcatct cgtacttctt ctttggcttg
     3061 cactaaatga taatcgacca aatgtcgtat tccccgtata gatggtttct cacgcatatg
     3121 ctcagctaaa aacaccgcag cttcgcgatc gccacgaaat cttttgtatt gtgccgcagt
     3181 cgctaaaacc acactgatgt gcgggcacga tttaataact tgatccaaat aacgtaacat
     3241 gtcttctttt gcgttcaatt tttcatagca ttgttgaatg ggaataataa cttcgcatac
     3301 aaaatcggga tcttgatact gcacctgctt atatgctttc aacgcttgct tgtatttacc
     3361 ttggcttttt tctatatt
//
LOCUS       DASTIB010000230         5856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1274224, whole genome shotgun sequence.
ACCESSION   DASTIB010000230 DASTIB010000000
VERSION     DASTIB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274224"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFP93_04015"
     CDS             complement(<1..355)
                     /locus_tag="VFP93_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843802.1"
                     /translation="MIRNFTRFFIFLIVTSLFVLSSCSPPYEERDVAVKDLQTKNQAL
                     ASAEEYLQTSYYHSPPKSFEYTLMAIEMFLEANEIASAKHWLHQVPAHVPEYLQIHRN
                     MLKAQINLQEKNFLAA"
     gene            378..1253
                     /gene="rsmI"
                     /locus_tag="VFP93_04020"
     CDS             378..1253
                     /gene="rsmI"
                     /locus_tag="VFP93_04020"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9843803.1"
                     /translation="MCADSNFNSKLYVVATPIGNLEDLSERAKKTLRSVNWIASEDTR
                     HSQGLLIHFGIQKRCYSLHNFNEKKQTSALIGLLKEGQTGALISDAGTPLISDPGFHL
                     IQAAHEAHIEVISIPGPCAAISALSVLAIQEGPIHFEGFLPAKKGSRIKRVSELRTLT
                     SVLVFYESPHRIEALFEDLCQVLGGERFCGIGRELTKKFETLYRDSLENLFKKLQDQT
                     IPIKGEFVIVVSGAQPIPQWEGDQRQLIQKLLTYLPITKAAKLAHEITGMDRKACYAL
                     ALSLQPKPPEEEDSI"
     gene            1320..1680
                     /gene="rnpB"
                     /locus_tag="VFP93_04025"
     ncRNA           1320..1680
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFP93_04025"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2265..2723
                     /gene="mraZ"
                     /locus_tag="VFP93_04030"
     CDS             2265..2723
                     /gene="mraZ"
                     /locus_tag="VFP93_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET9843804.1"
                     /translation="MFRGVNELSLDNKGRVAMPTRYRELILQEADNQLVLTIDTEEKC
                     LLIYPLPEWEKIERQIESLPSFNKAARRVQRLLIGHATEINLDSHGRFLIPPPLRDYA
                     QLDKGIILIGQGKKFELWNEGYWSKCREQWLQEEVKREDDLPPSLLSISL"
     gene            2720..3655
                     /gene="rsmH"
                     /locus_tag="VFP93_04035"
     CDS             2720..3655
                     /gene="rsmH"
                     /locus_tag="VFP93_04035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016929092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9843805.1"
                     /translation="MMVLEQHIPVLQNDVIQYLMHDPNGIYIDATFGRGGHATALLQR
                     LSPEGRLIVIDKDPQAIECAHKLFQKDERVLIHQGCFSQVLPLCRQLGCSGAVDGILF
                     DLGVSSPQVNTPERGFSFMKDGPLDMRMDPTTGMSAKEWINKAQTQEISLVLKRFGEE
                     KYSKRIAEKIVEKRGKKPITSTKELVEVIEHSIPHRESLKKHPATKSFQAIRIHINQE
                     LTVLETALQGALKVLAPHGRLCVISFHSLEDRIVKRFMRSLAHTQLPAHLPITAQEDK
                     RDLAILAKKIKPSAEEIANNPRSRSAILRVAQKIG"
     gene            3661..3969
                     /gene="ftsL"
                     /locus_tag="VFP93_04040"
     CDS             3661..3969
                     /gene="ftsL"
                     /locus_tag="VFP93_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET9843806.1"
                     /translation="MAKSAAKSKFERPYSRFSLWLVISMVIVIMLSSLGVIYSKHLSR
                     KYFAQLQSLQKQRDDLHVEWSQLLLEQGTWATDVRVERVAREHMYMTVPNPEDVKVIR
                     "
     gene            3966..5672
                     /locus_tag="VFP93_04045"
     CDS             3966..5672
                     /locus_tag="VFP93_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9843807.1"
                     /translation="MNSLQSPQQVTFSRHLVVWGVLLSFSVILSWRLTDLQIISDDFL
                     QEEGNKRSLRTIPLSSHRGLILDRNGEALAISTQVHSVWVNPKEIEDGFASKWNTLCE
                     LLSLDPSVAQAKILRKKDRSFVYLKRHISPQLAQKIDRLNLPGIHLQNEYRRFYPNAE
                     VSAHVLGFTDIDHRGQEGLEFALDSILSGSPGRKTILKDRLGAQIESREGNANPLPGQ
                     DVQLSIDSRIQYLAYRELLKAVKKNNAKAGSVVILDADTFEVIAMANVPSFNPNLIER
                     PLDGSARNRAVTDQFEPGSLIKPFSMAHILASGRFYPHSPVDTNPGWLKVGNRLVRDV
                     RNFGFIDVGTVLQKSSNVGITKLILSLPPEKLYKTYQDLGFGDTTHSGFPGEAMGHLE
                     YAGIKNPFALATLAFGYGISATPLQIAQAYAILAARGVKRPISYLKVEDTPPGEQVLS
                     PGIASAITRMLISATQTEGTAHNAYIPGYIVAGKTGTARKIGAQGYDEDSHLALFAGF
                     APAQDPKLVMVVIIDEPSAGRYYGGEVAAPVFARVMQGALRLSNIAPFRSDVPELLLV
                     KK"
BASE COUNT         1729 a         1151 c         1281 g         1695 t
ORIGIN      
        1 ctgcagctaa gaaatttttt tcttgtaaat ttatttgcgc ctttagcata tttctatgta
       61 tttgaagata ttctggaaca tgtgctggca cctggtgcaa ccaatgtttt gcactagcaa
      121 tttcattggc ttctagaaac atttcaattg ccattaatgt atattcaaaa gacttcggcg
      181 gagaatgata ataagatgtt tgcaaatatt cttcggcaga tgctaaagct tgattttttg
      241 tttgtaaatc tttgacagca acgtctcttt cttcataggg tggtgaacag cttgataaga
      301 caaataagct agttactatt aaaaaaataa aaaatctggt aaagtttcgg atcatatttt
      361 tcactacccc aaaatacatg tgcgcagatt ctaattttaa tagcaaactt tacgtagttg
      421 ctaccccaat tggaaatttg gaagatttat ctgaaagggc caagaaaact cttagatcag
      481 tcaactggat agcttcggaa gatacgcggc atagtcaagg tttgttaatc cattttggga
      541 tccaaaagcg ttgttattcg ttgcataatt tcaacgagaa aaaacaaacc agtgcgctca
      601 ttgggttatt aaaagaaggt caaacgggtg ctttaattag tgacgcgggt acacccttaa
      661 ttagtgatcc gggttttcac ttaatccaag cggcgcacga agcacacatt gaagtgattt
      721 cgatcccagg accctgtgcg gctattagtg ctttatcggt tttagcgatt caagagggtc
      781 ctattcactt tgagggattt ttaccggcga aaaaaggaag tcgcattaaa cgtgtatcag
      841 aacttcgcac tttaaccagt gttttggtct tttacgaatc tcctcataga attgaagcct
      901 tatttgagga tttgtgtcag gtgttgggcg gtgagagatt ttgtgggatt ggtcgtgaat
      961 taaccaaaaa atttgaaacg ctttatcgcg attctctcga gaatttgttt aaaaaattgc
     1021 aagatcaaac tatccccatt aaaggagaat ttgtgatcgt cgtatccggt gctcaaccta
     1081 ttccccaatg ggaaggcgat caacggcaat taatccaaaa attattaacg tatttaccca
     1141 ttaccaaagc agcaaaatta gcgcacgaga tcacgggaat ggatcgcaaa gcttgttatg
     1201 cgcttgcttt aagtttgcaa ccaaaacccc ccgaagaaga agactccatt tagcactcaa
     1261 actatctgtt tgttagaatg ttcttgcatc tttgaacact tctcagtatc gtgcacctgg
     1321 gagttggctg gacaatcgct gcgtaaattt ttttatgtgg aggaaagtcc gggctccata
     1381 gggcaaagtg ccaggtaacc cctgggaggc gcgagcctac ggaaagtgcc acagagaata
     1441 taccgcctaa attattaatt taggtaaggg tgaaaaggtg cggtaagagc gcaccgcatg
     1501 catggtaaca ttgcatggca cggaaaaccc cacttggagc aaaaccaaat aggaatccaa
     1561 agctttagct taagcgtggc ccacgctgga ttcgggtagg ttgctagagg taaatggtga
     1621 catttatccc agatgaatgg ttgtcccaga cagaacccgg cttataggcc agctcctttt
     1681 ttattatgat aagtttttcc tgcgcatgca ctacttcgta ttttcgcgcg ggcggaccta
     1741 gtcttgaaat ccttaaagat ggtagatccc ggatattaag cgacattaaa gctcgcaagc
     1801 ttacgattcc tccgtcgttc tcggcgcatg cgggaatctc actcttggat cccgggtatt
     1861 aagcgacata agctcgcaag cttgcaaatt tcatcttcgt tcccgcgcat gcggaaccca
     1921 gtgtaaccgc gtactcgttt gtacaagata caattcaatc ttaaaatcat tccgtttgtg
     1981 gtatagcgac atagggttgt gatctcaccc gaacaacacc ttatagggat aacttgtgtt
     2041 gcgcagcaga aattaatcgc attcttcttg atcttcgggc aaaatataac acttccaact
     2101 ttggaaaatt aacaaactaa ccagtgttaa aattttctaa gtgtttgtga aatagaggaa
     2161 ctttcaactt agtctgtaga ctttccttga caagtgggct gacccttacc tatagtgtca
     2221 gtaggtggta tgaagtggat cattgtggga acataggagt tttggtgttt agaggggtaa
     2281 atgagctcag tttagataat aaaggtcgag tggctatgcc aacccgctac cgagagctta
     2341 ttttgcagga agcagacaat caacttgtcc ttactatcga caccgaagaa aaatgtttgt
     2401 tgatttatcc tttgccagaa tgggaaaaaa tagagcgtca aattgagtcg ttgcctagct
     2461 tcaataaagc cgcaagacgc gtgcagcgac tgttaatcgg acatgccaca gaaatcaatt
     2521 tagattccca tggacgcttt ctcattccac cccctttacg cgactatgcg caactagata
     2581 aagggatcat attaattggg caagggaaaa aatttgaact gtggaacgaa ggctattggt
     2641 cgaaatgtcg tgaacaatgg ttgcaggagg aggttaagag agaggacgat ttaccaccct
     2701 cactcttatc gatatcatta tgatggtttt agaacaacac atacctgttt tgcaaaacga
     2761 tgttattcaa tatcttatgc atgatccgaa tggaatttat attgatgcca cgtttggtcg
     2821 tggtggacat gcaaccgctt tgctacaaag attgagtccc gaaggacgat taattgttat
     2881 cgataaggat ccgcaagcaa tcgaatgtgc acataagctt tttcaaaaag atgaaagggt
     2941 tttaatccac caaggttgtt ttagccaagt actaccattg tgcagacagt tggggtgttc
     3001 tggtgctgtc gatggaattt tgtttgacct tggtgtttct tctccgcaag tgaatacacc
     3061 cgaaagaggc tttagtttca tgaaggatgg accactggat atgcgcatgg atcctactac
     3121 gggtatgtct gcaaaagagt ggattaataa agcccaaacc caggaaattt ccttggtact
     3181 aaaacgcttt ggcgaagaaa aatattccaa aagaattgca gaaaaaattg ttgaaaaaag
     3241 aggtaaaaaa cctataacaa gtacgaaaga actcgtggaa gttattgagc actccattcc
     3301 gcatcgagaa tccttaaaaa aacatccagc gacaaaaagc tttcaagcaa ttcgaatcca
     3361 tataaaccaa gagttaaccg tattagaaac ggcattacaa ggtgcactaa aagtacttgc
     3421 accccatggc cgcctgtgtg ttatcagttt tcattctcta gaagacagaa tcgtgaagcg
     3481 ttttatgcgc tcgcttgcgc acacacaact tcctgcccat ttacctataa ccgcgcaaga
     3541 agataagcgc gatttagcta ttttggctaa aaaaattaag cctagtgcag aagaaattgc
     3601 aaataatcca agatcaagaa gtgctatttt aagagttgct caaaaaattg ggtgaaatgt
     3661 atggcaaaga gtgccgctaa gtctaaattt gaaaggcctt attctcgttt ttccctctgg
     3721 cttgtgatta gcatggtgat agtcattatg ctttcaagtt tgggggttat ctattccaaa
     3781 catttaagcc gtaaatattt tgcacaatta caatcattgc aaaagcaacg cgatgatttg
     3841 catgtggaat ggagtcaatt attattggaa caaggaactt gggcaacgga cgtgcgtgtt
     3901 gagcgtgtgg caagagaaca catgtatatg accgtaccca atcccgagga tgtaaaggtt
     3961 atccgatgaa ttccctgcaa tcccctcagc aggttacttt ttccagacat ttagtcgttt
     4021 ggggagtttt gttatctttt tcggttattc tttcttggcg tttaacggat ctgcaaatta
     4081 taagcgacga ttttctgcaa gaagaaggca acaaacgatc gttacgtacc attccattaa
     4141 gctcgcatag gggccttatc ttagatagaa atggcgaagc cttagcaatt tcaacccaag
     4201 tacattccgt ctgggttaat cccaaagaaa tagaggacgg ttttgcctca aaatggaata
     4261 cactgtgtga attattatcc ttagatccaa gtgtcgccca agcaaaaatt cttcgcaaaa
     4321 aagatcgttc ttttgtttat ctaaaacgcc atatttctcc gcaattagct caaaaaatag
     4381 atagactaaa tctacccggt atacatttgc aaaatgaata tcgccgtttt tatcccaatg
     4441 ccgaagtcag tgcccatgtt ttaggtttta ccgatattga tcataggggg caagaagggt
     4501 tggagtttgc tttagactct attctctctg gaagtcctgg cagaaaaacc attttaaaag
     4561 ataggctagg tgcacaaata gaaagtcgcg aaggtaatgc taacccgttg cctggccaag
     4621 atgtgcaatt gagtatcgat tcaagaattc aatatcttgc ctatcgtgaa ttgttgaaag
     4681 cggtgaaaaa aaataacgcc aaagcaggat ccgtcgttat tttagatgcc gacacctttg
     4741 aagtcattgc tatggcgaat gttccttcgt ttaatcccaa tttaattgaa cggcctctgg
     4801 atggtagcgc tcgcaatcgc gcggtaaccg accagtttga acctggttct ttaattaaac
     4861 cttttagtat ggcgcacatt ttggcaagtg gtcgctttta tccacattct cccgtagaca
     4921 caaatcccgg ttggcttaaa gtgggtaacc gtttggtaag agatgtaaga aattttgggt
     4981 ttatcgatgt gggtacggtc cttcaaaaat cgagtaacgt agggatcacg aaattaattt
     5041 tatctttacc accggaaaag ctctataaaa cttaccaaga cttaggtttt ggtgacacaa
     5101 cccatagtgg ttttccaggg gaagcaatgg gacacttaga atatgccggt ataaaaaatc
     5161 cttttgcttt ggcaacctta gcatttggct acggtatttc cgccacccca ttacaaattg
     5221 cgcaagctta cgctatctta gctgcacgcg gtgttaaaag acccatcagt tacttgaaag
     5281 ttgaagatac gccacccggt gaacaagtac tatcacctgg tattgcttca gcaatcacga
     5341 gaatgttaat ttcagccacg caaacggaag gtactgcgca taatgcttac atacccggtt
     5401 atatcgtcgc agggaaaaca ggtactgcaa gaaaaatagg tgctcaaggt tacgatgaag
     5461 atagtcattt agctttattc gctgggttcg cgccggcgca ggatcctaag cttgttatgg
     5521 tggttattat tgatgagccc agtgcaggtc gttattatgg cggtgaagtt gcagcccctg
     5581 tttttgcgcg tgtcatgcaa ggagctttgc gattatcgaa tattgcacct tttcgtagtg
     5641 atgtgcctga acttttgttg gtgaagaaat aattaaatgg acgctcaaac cctgtttagc
     5701 ccttttgtac gagaatcctt gccatggaat tttatggtta atggattatc aaaccatagt
     5761 aaaaatattc aaccggggga agtatttttt gcttatcctg gggccacacg cgatggcaga
     5821 ttgtatattc cagaagtttt aacaaaacag cctgcg
//
LOCUS       DASTIB010000231         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1397080, whole genome shotgun sequence.
ACCESSION   DASTIB010000231 DASTIB010000000
VERSION     DASTIB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397080"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..2975
                     /locus_tag="VFP93_04050"
     CDS             198..2975
                     /locus_tag="VFP93_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843808.1"
                     /translation="MSQQGPHPKKHKSNAHEQKISIVSRWYIKFQGADNYLIENIGDL
                     ECIEKALECGANVNPKSPDAVSPLLQAIAKNAPVSVKKLIKAGADVNLPGKVLLAESY
                     SNSTLPIMKALELGNAKIVYYLLRSKAKLDFTYAIKNAVVQGNNPVLEILIQSIAHEE
                     AVLLVNKLTPALEFALNKKKLQALCLLMQYYPCKEQFVQKALTTGNKELLTTLVDLNI
                     DLNQPVYFSQFNETLTPFDFLLRNKDAEMLDFLLELNVALLDYDKESLVDLYASLNDS
                     QKTGYFKKLIVPFLKNLEIDGELKLPVLPFLNINNCIHLSKELLLLLLNNPTEFINMH
                     SKSELHHLFVPARLKAREILHASQAIDRERVRMQNALANNNESAMHDDNKLFTHYSRT
                     IKPHYQSEFLRRGGVAFIEAEIRGLLLRRILANGILPDFGKEIIRQNLDILVSGEEKE
                     AMDLIRPLLNSNTDSNQVAWRLYDKWAPFIGERNFMTEEPSGFGTNVREMIAYYFIGV
                     MDVNMQPECKEDRVANFILQLSEIRRGNNKDNPSEPMDVISCLTGYRSRIGQMGILHP
                     ILSLPELPLEEIMKNLVAAKFHEYLRTIPAYDDKARELLFNALVMLQESNAQETISGE
                     VFYSDEMLNVREAFIVDALGDRFNDIYREVEKQLISYMHGEDFMREMERPINQLKLQL
                     YRLDVSRGTIGDRLTELYNPYRERAITIEKIEALLKHILLNGVHKPHLRPIIYNFLDV
                     EQKTSEVFVDFFREVLENSNFIDDDIKKMHQIYLNNKDLLKALRPICQENLDYLKNYS
                     NRYLGSQDFLMTSLQDVFVKRKLQMAHYPEELKKTQIHKMNRQQERCIRVAESLCGEC
                     PNDLLKYQYLNLKNVIVDFIEYTTNQAIDELDEKFWNDFKTKHPIINQLGEDAITNFT
                     TEKPRFKRAYF"
BASE COUNT         1043 a          488 c          540 g          920 t
ORIGIN      
        1 atgacaggtt caaaattgtc tcatattttt ccctaattat cggccaaatc gttaaggtaa
       61 taacttttct ttttggcagc aaaaacagat agtattttat tatcgtacca attctacctc
      121 ctatagtatt ttcgttgtta ctaactctga aagtttttat cctcaaatca atattaaaaa
      181 catagcgaga gaaatgtatg agtcaacaag gtccgcaccc caaaaaacat aaatcaaatg
      241 cacatgaaca aaaaatcagc attgtttcca gatggtatat caaatttcaa ggtgcggaca
      301 attatttaat tgaaaacatt ggcgatttag aatgtattga aaaagcatta gaatgtgggg
      361 cgaatgtaaa tcctaaatcg cctgatgctg tatcgccttt acttcaagca attgcaaaaa
      421 acgctcccgt aagcgttaag aagcttataa aagcaggggc ggatgttaat ctccctggaa
      481 aggttcttct tgctgaaagt tattctaata gtacattacc tataatgaag gcacttgaac
      541 taggtaatgc aaaaattgtt tactatttac ttaggtcaaa agcaaaactc gactttactt
      601 atgctataaa aaacgcagta gtgcaaggaa ataatcctgt tttagaaatc ttaattcaat
      661 cgatagcgca cgaagaagct gtactgttag taaataagtt aactccagct ctagaatttg
      721 ccctaaataa gaaaaaatta caagcattgt gtttattaat gcaatattat ccctgtaaag
      781 agcaattcgt gcagaaagcg ttaactacgg ggaacaaaga attacttacg acattagttg
      841 atttaaatat cgatctaaac caaccggtat atttttcgca atttaatgaa accttgaccc
      901 cgtttgattt ccttttaaga aacaaagatg ccgaaatgct tgactttttg ttggaattaa
      961 acgttgcact tttggattac gataaagaat ctctcgtgga tctctatgct tccttaaacg
     1021 atagccaaaa aacaggttac ttcaagaaac ttattgtacc cttcttgaaa aatttagaaa
     1081 ttgatggtga actaaaactt cccgtgttac cttttttaaa tattaataat tgtatacatc
     1141 tttccaagga attattgtta ttgctattaa ataatccaac agaatttatt aatatgcatt
     1201 ccaaaagtga attacatcat ctatttgttc cggcacgttt aaaagcccgt gaaattttac
     1261 atgcatccca agctatcgat cgtgaacgtg taaggatgca aaatgcttta gctaataata
     1321 acgaatcagc gatgcatgat gataataagt tatttaccca ttattcgcgt actataaaac
     1381 cccattatca aagtgaattc ttgcgtcgcg gaggggttgc gtttattgag gcagagatta
     1441 gaggtttact tctaagaaga atcttggcga atggcatttt accggatttt ggtaaagaga
     1501 tcattcgaca aaatctagat atattagtgt ctggagagga aaaagaagcc atggacttaa
     1561 ttcgtccctt gctgaattca aataccgata gcaatcaagt tgcttggcga ctttacgata
     1621 aatgggcgcc gtttatcggt gaacgtaatt ttatgacgga agaaccttca ggattcggaa
     1681 ccaatgtaag agaaatgatt gcctattatt ttataggtgt tatggatgta aacatgcaac
     1741 ctgaatgcaa agaagacaga gttgcaaatt ttattctaca actttcagaa attcgtcgtg
     1801 gtaataataa agataatccc tctgagccta tggatgtgat ttcttgctta accggatatc
     1861 ggtcgcgtat aggtcaaatg ggtattttac atcccatatt gagcttgccc gaattgcctt
     1921 tagaagaaat tatgaaaaat ttagttgcgg ctaagtttca cgagtattta cgaactattc
     1981 ctgcgtacga tgacaaagcg cgtgagttgc tttttaacgc attggttatg ctgcaagagt
     2041 cgaacgctca agaaacaata tcaggggaag tgttttattc agatgaaatg ctaaatgtac
     2101 gagaagcatt catcgtagat gcattaggcg atcgatttaa tgatatttat agggaggtag
     2161 aaaagcaatt aatttcgtat atgcacggtg aagattttat gcgagaaatg gagcgaccga
     2221 ttaaccaatt aaaactacaa ctttatcgct tagacgtaag tcgtgggaca ataggagata
     2281 gattaacaga gctctacaat ccgtatcgtg aaagagctat tacgatagaa aaaatagaag
     2341 ctcttttaaa acacattcta ttaaacggtg tgcataaacc acacttgcgt cctatcattt
     2401 ataatttttt agatgttgag cagaaaactt ctgaagtttt tgtagacttc tttagagaag
     2461 ttctcgaaaa ttcaaacttt attgatgatg atattaaaaa aatgcatcaa atttatttaa
     2521 acaataaaga tctacttaaa gcattaagac ctatttgtca agaaaatttg gattatctga
     2581 aaaattatag caatcgatat ttagggtccc aagattttct tatgactagc ttgcaggatg
     2641 ttttcgttaa aagaaaacta caaatggcac attaccctga agagcttaag aaaacgcaaa
     2701 tacataagat gaacaggcaa caagaacgtt gcatccgtgt agcagaatcg ttgtgtggcg
     2761 aatgtccaaa cgatttgtta aagtatcaat acttaaattt aaaaaatgtt attgtagatt
     2821 ttatagaata cacaaccaat caggctatcg atgaattaga tgaaaaattc tggaatgatt
     2881 ttaaaacaaa gcatcccata atcaatcagt taggtgaaga tgcaataaca aattttacta
     2941 cagaaaagcc tcgattcaaa cgtgcctatt tttaaaattt aagacgcgct c
//
LOCUS       DASTIB010000232         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_689965, whole genome shotgun sequence.
ACCESSION   DASTIB010000232 DASTIB010000000
VERSION     DASTIB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689965"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2314
                     /locus_tag="VFP93_04055"
     CDS             <1..>2314
                     /locus_tag="VFP93_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HET9843809.1"
                     /translation="EDNQPLKGLLYIKARDQNLELFKNMLKQSPITVIGDLDLEMWNT
                     VKGFEILQSTLRFDANKLHLKSPQEAYYIDSLRGSVVYNKEEGQNRYQLIAKDLDFGA
                     DPKTSLFLEAMDFDTFSLKGSNINLAFLHFLVNGTSLSESLTNLQVKPHGLIPYFEAT
                     IDKNFRVKDLLLEGENLGFVHPKLKAYGLDGKFFLTENTMQADLKGHNLFVPESTYFS
                     QEFVIPSYQMHLVFDLYRQTIQLYNIFELPKGVIEQKLKVHVQNDVILSIDMHASTLS
                     VEALKTFVPSKIFNPSLQLWLEKALKSGDFSDISFKYFGLAQDLEKKNTTPIRLKANV
                     HNLTLDYAPPWPTLRQFEGQLSIDNHQLTMQGKGALPQGEVTNIAAIIPDLRAEPTNL
                     QIEAVVKADLTYGWNVLKESTILANDVYKMFDFRGKSVATLNFNIPLTEDKNYKTQYN
                     GTIHFDSATAQLSELHEPFQQMQGVINFQNELIKLENITTQWFNNPLQGNIMLTLGEN
                     PHLTIQSEGTLALKNQFENAFVKGDVPFTAAIDWYLKDQNFADAHIIADLTQVDSKLP
                     EPFDRLKSKSPLKIQTYFKPKDQIKMQLSLATALKAALKFDLKPTTGMRFEAGHIHFG
                     SEDPIELPIQGWKIDGVLDTIHLTDWLKLKNNFPTNPHLPIEFDLFVHTLGMQNNHFS
                     KMNIQGAFAVNANKWQIQIHSPQLEASISLPLDPNKAWEFAVSNVILEKSFENKDQQE
                     YPRRPLIFTIEKLQRPGMRIKDFSLQLFPN"
BASE COUNT          804 a          413 c          425 g          672 t
ORIGIN      
        1 ttgaggacaa ccaaccatta aaagggttgc tctatattaa ggctcgtgat caaaatctcg
       61 aactttttaa aaatatgctg aaacaaagcc ccattacagt tataggggat ctagatttag
      121 aaatgtggaa taccgtaaaa gggtttgaaa tactccagag caccttgcgt tttgatgcta
      181 ataaattaca tttaaaatcg cctcaggagg catattatat cgattcattg cgtggaagcg
      241 tcgtatataa caaggaagag ggtcagaatc gttatcaatt gattgcgaaa gacctagatt
      301 ttggcgcaga tccaaagact tccctatttt tagaagcgat ggattttgat actttttcac
      361 ttaaaggtag caatataaat cttgcttttt tgcattttct tgttaatggc acgtcgctgt
      421 cggaaagttt aacaaacttg caggtaaaac ctcacggctt aattccttat tttgaagcaa
      481 cgattgataa aaactttagg gttaaagatt tattattgga aggggaaaat ttaggttttg
      541 tgcatccaaa attaaaagct tatggactcg acggtaaatt tttcttaacc gaaaatacca
      601 tgcaagccga tctcaaagga cacaatcttt ttgtgccaga aagcacatac ttttcacaag
      661 aatttgttat acctagttac caaatgcatt tggtctttga tttataccga caaacgatac
      721 aattgtataa tatttttgaa ctacccaaag gtgtaattga gcaaaaattg aaggtacatg
      781 tacaaaatga tgtaatttta tctatagaca tgcacgccag tacactaagt gtggaagcgc
      841 taaaaacctt cgtacctagt aaaatattta atccatcgtt acaactgtgg ttagagaaag
      901 cattaaaatc gggagatttt tcggatattt cttttaaata ttttggcttg gcgcaagact
      961 tggagaaaaa gaatacaacc ccgattaggt taaaagcaaa tgtgcataac cttacgctag
     1021 attatgcacc tccgtggcca acacttcgtc agtttgaggg gcaattatcg attgataatc
     1081 atcaattaac catgcaagga aagggtgctt taccgcaagg agaggtgaca aacatcgctg
     1141 caattatacc tgatctacga gcagaaccta ccaacttaca aatcgaagct gtggtaaaag
     1201 cagacttaac ctacggctgg aatgtgttga aagaaagtac gatccttgca aacgacgtat
     1261 ataaaatgtt tgattttaga ggaaaatctg ttgccacttt aaatttcaac attcctctta
     1321 cggaagataa aaattataaa acgcaatata atggcactat tcatttcgat tctgctacag
     1381 cacaattatc cgaactacat gagcctttcc aacaaatgca aggggttatt aactttcaga
     1441 atgaactaat taaactagag aatataacaa ctcaatggtt taataatcct ctgcaaggaa
     1501 acataatgtt aacgttagga gagaacccgc atttaactat ccaatcggaa ggtactctag
     1561 cactcaaaaa tcaatttgag aatgcttttg taaaaggcga tgtacctttt acagctgcta
     1621 tcgattggta tcttaaagat caaaattttg cggatgcgca tattatcgcc gatctgacac
     1681 aggtagatag taagttgccc gaaccttttg ataggttaaa atcaaaaagt cccttaaaaa
     1741 ttcaaaccta ttttaaacct aaagatcaaa taaagatgca attaagctta gctacagcgt
     1801 tgaaagctgc attaaaattt gatctaaaac ctaccacggg aatgcgattt gaagcgggcc
     1861 atatccattt tggcagtgag gatccgatag agctacccat acagggttgg aaaatagatg
     1921 gggtgcttga tacaatccat ctcaccgatt ggctaaaact taaaaataat tttcctacca
     1981 acccccattt acctatagag tttgatttat ttgtgcatac gttgggaatg caaaacaatc
     2041 acttttcaaa aatgaatata caaggtgcat ttgcagttaa tgctaacaaa tggcaaatac
     2101 aaatccatag tccgcagcta gaagcatcga tatcactgcc gttggatccc aataaagcct
     2161 gggagtttgc ggtaagtaat gtaattttag aaaagagttt tgaaaacaaa gatcaacaag
     2221 aatatcctcg acgcccgtta atttttacga tagaaaaatt acaacgtcct ggtatgagaa
     2281 taaaagattt tagcttacaa ttattcccca atga
//
LOCUS       DASTIB010000233         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1033685, whole genome shotgun sequence.
ACCESSION   DASTIB010000233 DASTIB010000000
VERSION     DASTIB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033685"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1511
                     /gene="gshA"
                     /locus_tag="VFP93_04060"
     CDS             210..1511
                     /gene="gshA"
                     /locus_tag="VFP93_04060"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET9843810.1"
                     /translation="MHWKIIMKPAVFGVPHLKTIQDQLRFDLEKKIVKHQVEIECWFR
                     GQWLKHKPLFYCSVDLRNAGFKLAAVDTNVFPGGFNNLNPDFLPLCVQAATQTLSQLY
                     PNCNNILILAESHTRNPHYLKSLHILQTILTQAGFNIRIGTLRPIDDKETFNLPDATI
                     VQWPIVRKDNRIVCADFEPCIVLLNNDLSEGVPEILQNLKQPIIPELTMGWNIRSKYD
                     HFLHYELNASKLASILNIDPWLIVPLFSQCIDVDFLSSEKDACIIEKAEELFEAVVKK
                     YKEYQIKHKPFLVIKSDAGTYGMAVLMVKEVKEILNLNRKQRTKMSTRKGGMQVDKVL
                     IQEGVYTMEAKENQATAEPVVYMIGSYVIGGFYRIHTGRGMQDNLNAPGMHFEPLAFD
                     TCCNTPDERLSPHDSKNQFYAYSVIARLAHLATRHEQGSSK"
     gene            1508..>2319
                     /gene="gshB"
                     /locus_tag="VFP93_04065"
     CDS             1508..>2319
                     /gene="gshB"
                     /locus_tag="VFP93_04065"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET9843811.1"
                     /translation="MTKKLIVLMDPIEKIKVHKDSTYALMREAESRGWLIYYMQLTDL
                     ILQNGMICAQTQQISFKEDDTNWYRYLSSKETAPLQSFDIFFMRKDPPITASYIYATQ
                     LLEMAQKQGLLVVNDPRALRDVNEKIFATRFKELCPETLITAKKNLIHDFLRDQKKVI
                     LKPLNGMGGDSIFFLERGNPNFHVIVDILTVQETVPIVVQRFLAGIKKGDKRIFIIDG
                     KPYPYGLKRIPPKGEVRANLAAGGTGVGFALTKKDMQIADGLSETLKKLGLI"
BASE COUNT          783 a          404 c          434 g          698 t
ORIGIN      
        1 tgttttttga gcatctaact caaacgtaag atatacgctg tgaatatggt agtgtaatgg
       61 ctgaaaatct tttctatatt taattttttg catgttaatc ccttacttta tccataaagt
      121 tgtacatttt tttctaccat ggctgtactt tattacaagt gactctaacc ttgcaaggat
      181 aaacaatatg acaaataatg gtaaagaaga tgcattggaa aataattatg aaacctgccg
      241 tcttcggtgt accgcattta aaaaccattc aagatcaatt gcgttttgat ttagaaaaga
      301 aaatcgtaaa gcaccaggta gaaatcgaat gctggtttcg tggccaatgg ctgaagcata
      361 aaccattatt ttattgttca gtcgatttac gcaacgcggg tttcaaactg gctgcagtgg
      421 ataccaatgt atttcctggt ggatttaata atttaaatcc agatttttta ccactttgtg
      481 ttcaagccgc gacacaaaca ttaagtcaat tatatcctaa ctgcaataat attttaattt
      541 tagctgaatc gcatacacga aatccgcatt atttaaaaag cctgcatatt ctacaaacaa
      601 tccttacgca agcgggattt aatatacgta taggcacgct gcgacccatc gacgacaagg
      661 aaacgtttaa tttaccagat gcaactattg ttcaatggcc cattgttaga aaagacaata
      721 gaatcgtttg tgcagatttt gaaccttgta ttgtgctgtt aaataatgat ttatcggaag
      781 gtgttccaga aatattacaa aatcttaaac aacctataat tcctgaactt actatgggtt
      841 ggaatatccg aagcaaatac gatcatttct tacactatga attaaatgca tcaaaattag
      901 cttctatttt aaatattgat ccttggctta ttgttccact attttcccaa tgcattgatg
      961 ttgatttttt aagcagcgaa aaggatgctt gtatcatcga aaaagccgag gaattgtttg
     1021 aggctgttgt caaaaaatat aaagaatacc aaattaaaca caaacccttt ttagttataa
     1081 agtcagacgc aggcacgtat ggtatggcag ttttaatggt aaaagaggtt aaggaaatac
     1141 tcaacttaaa tcgcaaacaa cgtaccaaaa tgagcacgcg taaaggtgga atgcaagtgg
     1201 ataaagtatt aatccaggaa ggtgtataca ccatggaagc aaaggaaaat caggcaaccg
     1261 cagaacctgt tgtttatatg attggttctt atgtaatagg aggattttac cgcatacata
     1321 ccggtcgagg aatgcaagac aatctaaatg cacctgggat gcattttgaa ccgttagcat
     1381 ttgacacttg ctgtaacaca ccggatgaaa gattaagccc gcacgattca aaaaaccagt
     1441 tttatgctta tagtgtgata gcgcgtctgg cacatttggc aactcgtcac gaacaaggat
     1501 ccagcaaatg actaaaaaat taatcgttct catggatcct atagaaaaaa ttaaggtgca
     1561 taaagactca acctatgctt tgatgcgaga ggctgaatcc agaggttggt taatttatta
     1621 tatgcaattg acggatctaa ttctacaaaa tggcatgatt tgtgcccaaa cccaacaaat
     1681 aagttttaaa gaagatgata caaattggta tcgttatcta agttcaaaag aaacagcgcc
     1741 tttgcaaagt tttgatattt tttttatgcg caaagatccc cctataactg catcgtacat
     1801 ttatgcaaca caacttcttg agatggcgca aaagcaaggt ttacttgttg tcaacgaccc
     1861 aagagcactg cgtgatgtca atgaaaaaat ctttgcgaca aggtttaaag aattatgccc
     1921 tgaaactctt attacagcaa aaaaaaacct aatccacgat tttttacgcg atcaaaaaaa
     1981 agttattctt aaacctctta atggtatggg aggggattcc attttttttc tggaacgtgg
     2041 caatccaaac tttcacgtga ttgtcgatat tttaaccgta caagaaacgg ttcctattgt
     2101 ggtgcaacgt tttttagcag gtataaaaaa aggggataaa cgtattttta ttatagatgg
     2161 taaaccttac ccgtatggat tgaaacgaat tccacctaag ggagaagtgc gcgccaattt
     2221 ggcagcagga ggtacaggcg ttggttttgc acttaccaaa aaagatatgc aaatagccga
     2281 tggtttaagt gaaaccttaa aaaaattagg tcttatctt
//
LOCUS       DASTIB010000234         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_330381, whole genome shotgun sequence.
ACCESSION   DASTIB010000234 DASTIB010000000
VERSION     DASTIB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330381"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..934
                     /locus_tag="VFP93_04070"
     CDS             152..934
                     /locus_tag="VFP93_04070"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006794045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET9843812.1"
                     /translation="MCELMGMSANIPTDICFSFTGLMERGGRTGPHKDGWGVAFYEGR
                     GCRTFHDPFPSSHSEIAKFIKNYPIKSKTVICHIRKANRGRVCLENTHPFVRQLWGRN
                     WCFVHNGQLKGIKRSLKLKHFFPVGTTDSEHAFCWIMDRLHEKYPKKPPARKETLWKF
                     IEELCHKIAEHGVFNILLSDCRYLYAHCSTKMVWITRKAPFGKAKLIDAEMVVDFEKH
                     NSPNEIITVIATAPLTDNELWFQAEKKEFLVFEDGKLKNKNA"
     gene            complement(935..2461)
                     /gene="glpD"
                     /locus_tag="VFP93_04075"
     CDS             complement(935..2461)
                     /gene="glpD"
                     /locus_tag="VFP93_04075"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HET9843813.1"
                     /translation="MDIKFVDLLILGGGINGTGIAADAAGRGLHVVLCDKKDLGSGTS
                     SASSKLIHGGLRYLEHYALKFVKRSLREREILLHSAPHLIHPIPFILPHNPKLRNFWV
                     VRLGLFLYDILKAPSRLPFSTLVRFDHRQKNHPLSRSYARGFQYYDCMADDSRLVIAN
                     ALRAKDHGAHIMPYTEVLQIERKPAWWRILLRKDGTTFEVHAKACVNATGPWALETLH
                     KLFLLSSSVQIKLIKGSHIIVPKLYEEDVAYLLQNKDARIIFVLPFQKQFSLIGTTDI
                     VVDSPNQENTSSLEEIEYLCQCVSSYFHHPIKISDVIHSFCGIRTLVGTQNNPPASLS
                     REHHLELHSESPDSPPLLTIFGGKITTFRILAEEALEVLSPYLPTMGKPWTRNSILPG
                     GLIGKHSMDSFIETVKQSFPFLPFSLVERYAHAYGSSLKNVLANVRSMNDLGEHFGHG
                     LYEVEVKYLLDQEWATHADDILWRRTKLGLLFSPHEIQRLNQKISFYLSTKMHDKMVI
                     "
     gene            2647..>4080
                     /locus_tag="VFP93_04080"
     CDS             2647..>4080
                     /locus_tag="VFP93_04080"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HET9843814.1"
                     /translation="MIYYDLYPHRPKSHVFKVVMRVEPSCAKSETFFLPAWLPGSYMI
                     RDFAKHIINIQGYLAHNPQVPVPIRKIKTHTFEVQPWEGTLILEYEVYAWDRSVRGAH
                     FDLTHAFFNGSALFLCSNSFQDHSVQLVLHRPQETYQAKAWKVATSLKPLAIDAAGFG
                     SYSAQNYEDLIDHPTEMGTFEEVAFTLQDVKHRIIITGLQESDLERLAYDVKKLCEQQ
                     VNLFQDLPFENYVFLLTALEESYGGLEHLSSSALICQKRCLPSKNGFSLKDYRILLGL
                     FSHEYFHAWMVKRIKPQEFYDLNLREIHHTDQLWAFEGFTAYYDDLMLFRAQCITPFE
                     YLEVLSETMNRVYSTPGRFIQTLKESSFDAWIKFYQPNENSINSTINYYTKGTLAGLL
                     FDLHIRKISNNQYSLDDVMRKLWQDYGRNNIGVAPDAIEKILLEFGGPAMQDLIYLVL
                     ETTQELPLAQLLQDFGIALTYHYSEELK"
BASE COUNT         1278 a          766 c          807 g         1229 t
ORIGIN      
        1 gtgtgaaagg tttctttata agtatcataa cttcaagttt aggtggtata attccagctg
       61 cgatagccgc taacagtcag ccgactaagc tacttgttta tcaataatag ctgagcctac
      121 aatattttac aaaatttgag tttgaataac gatgtgtgaa ttaatgggta tgagtgcaaa
      181 tattccaaca gatatttgct ttagctttac cgggctcatg gaacgaggag ggcgtacagg
      241 tccacataaa gatggatggg gagtggcatt ttacgaaggg cgtggatgcc gtacttttca
      301 cgatcccttt ccaagcagtc actctgaaat tgccaaattt attaaaaatt atcccataaa
      361 aagcaaaacg gtcatttgtc atatacgtaa agcaaatcgt ggtagagttt gtcttgaaaa
      421 tacgcatccc tttgtgcgcc aactttgggg tcgtaattgg tgttttgtgc ataatggaca
      481 attaaaaggc attaaacgca gtttaaaact gaaacatttt tttccggtag ggacaaccga
      541 ctcagagcat gcattttgtt ggatcatgga cagattacat gaaaaatatc ccaaaaaacc
      601 gccggcgcgc aaagagactt tatggaaatt cattgaagaa ctttgccata aaatcgctga
      661 acatggcgtt ttcaatattt tattaagtga ttgcagatat ctttatgcgc attgttcaac
      721 aaaaatggtt tggattacgc gaaaagcacc ctttggcaaa gcaaaactta tcgatgccga
      781 aatggtagtg gattttgaaa aacataattc tccgaatgaa atcattacgg tgattgcaac
      841 tgcacccctt acagacaatg aactttggtt tcaagcagaa aaaaaagaat ttctcgtatt
      901 cgaggatggc aaattaaaaa ataagaatgc ttaattatat gaccatttta tcatgcattt
      961 ttgtgctgag ataaaatgag attttttgat taagtcgctg aatttcatgc ggcgaaaata
     1021 ataatcctag ttttgtacga cgccaaagaa tatcgtctgc atgcgtagcc cattcttgat
     1081 ctaataaata tttcacttct acttcataca acccgtggcc aaaatgttcc cccaaatcat
     1141 tcatacttcg gacattggct aaaacatttt ttaaagaaga gccataggcg tgtgcgtacc
     1201 gttccaccag cgagaaaggc aaaaagggaa aactctgttt tacggtttcg ataaatgaat
     1261 ccatggaatg cttccctatt aagcctccag gcaaaatact gttacgtgtc caaggctttc
     1321 ccattgtagg aagatagggg gataaaactt ctaaagcttc ttctgccaat atacgaaaag
     1381 tagtaatttt cccgccaaaa atcgtaagta aggggggact atccggagat tcactgtgaa
     1441 gttcaagatg atgttctctc gataggctag caggagggtt attttgtgta ccgaccaaag
     1501 tacgaatacc gcagaagcta tggatgacgt ctgagatttt aataggatga tgaaaatagc
     1561 tggaaacaca ctgacataaa tattctattt cttctaagga actggtattt tcttgattcg
     1621 gagaatccac aacgatatcg gtggttccaa ttaagctaaa ttgtttttga aatggcaaaa
     1681 caaaaataat acgcgcgtcc ttattttgta aaagatacgc cacatcttct tcatataatt
     1741 taggcacaat gatatgactg cctttgataa gtttaatttg cacagaagag gataataaaa
     1801 aaagtttatg caatgtttcg agtgcccacg ggccggtggc gtttacgcac gctttagcat
     1861 gcacttcaaa ggtcgtgccg tctttgcgca ataaaatgcg ccaccatgct ggttttcgtt
     1921 caatttgtaa cacttcggtg tacggcatta tgtgtgcgcc atgatcttta gcgcgcaatg
     1981 cattggcaat aacaagacgc gaatcatccg ccatgcaatc ataatattga aagcctcgcg
     2041 cgtaggaacg acttaatgga tggttttttt gtctatgatc aaatcgcaca agagtagaaa
     2101 aaggtaatcg tgaaggtgct tttaaaatat cgtacaaaaa caaacctaaa cgcaccaccc
     2161 aaaaatttcg caattttgga ttgtgtggca gaataaaggg tattggatgg atcaaatgag
     2221 gtgcactatg gagtaaaatt tcacgttctc ttaaagaacg ttttacaaat ttaagtgcat
     2281 aatgttcaag ataacgtaaa ccaccatgaa taagcttgct acttgcagaa gaagtgccac
     2341 tgcctaaatc ctttttatca cacagcacaa catgcaaccc tctgcccgca gcatccgccg
     2401 caatgccggt accgttaata cctccgccta aaatcaacaa atcaacgaat ttaatatcca
     2461 tattttagct atcggccttt tgttattgtt ataccttggt tatggtttat gtaagatata
     2521 tatataaccc gatttttaac ggtgctataa tttgttgaag ttataacaat agccaccctt
     2581 ttatgagtgt aacacgattt tttacacatt cgcaggataa atctaattgt cgtggatgga
     2641 ggatttatga tttattatga tttgtatccg catcgaccga aatcacatgt ttttaaagtc
     2701 gtgatgcggg tagagccctc ctgcgcaaaa tcagaaacct tttttttacc agcctggcta
     2761 cctggcagtt atatgattcg cgattttgct aaacatatca ttaatataca aggttattta
     2821 gcgcataacc cgcaggtgcc tgtacccatt cgtaaaatta aaactcatac atttgaagtg
     2881 caaccctggg agggaacatt aattttggag tacgaagttt atgcttggga tcgatcggta
     2941 cggggtgcgc attttgatct aacgcatgca ttttttaatg gttcggctct atttctttgt
     3001 tctaattcct ttcaggatca cagtgtacag cttgtgttac atcgacctca ggaaacctat
     3061 caagccaaag catggaaagt ggcaacgagt cttaagcctt tagcaattga tgcggcagga
     3121 tttggatcct atagcgcaca aaattacgag gatttaattg atcatcccac agaaatgggt
     3181 acttttgaag aggtagcttt tacgttacag gatgtaaaac acagaataat cattacagga
     3241 ttacaagaga gcgatttaga acgtttagct tacgatgtca aaaaactatg tgaacaacag
     3301 gttaatttat ttcaagatct tccgtttgaa aattatgtgt ttttattaac ggctttggaa
     3361 gaaagctacg gtggtttgga acatttatcg agctccgcat taatttgtca aaagcgatgc
     3421 ctgccctcca aaaatgggtt ttccttgaaa gattatcgaa tattactcgg tttatttagc
     3481 catgaatatt ttcatgcctg gatggtaaaa agaattaagc ctcaagaatt ttatgatctt
     3541 aatttgcgcg aaatccacca taccgatcaa ttgtgggcct ttgaaggttt tactgcatat
     3601 tatgatgatt tgatgttgtt tcgcgcgcaa tgtatcacac cctttgaata tttagaggta
     3661 ttaagtgaaa ccatgaaccg tgtgtattcc acccccggtc gttttatcca aaccttaaaa
     3721 gaatcaagct ttgatgcgtg gatcaaattt taccaaccca acgaaaattc cataaacagt
     3781 acaattaatt attatactaa aggtactttg gcaggtttat tgtttgatct gcatatccgg
     3841 aaaatttcca ataaccagta ttctctagac gatgttatgc gaaaattatg gcaagattat
     3901 ggtcgtaaca acatcggtgt agctcccgac gccatcgaaa aaattctctt agagttcggt
     3961 gggcctgcta tgcaagatct catttatctc gtgttagaaa caacacagga actaccactt
     4021 gcgcaactac tacaagattt tggaattgcc ttaacctacc attacagcga agagctaaaa
//
LOCUS       DASTIB010000235         4337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_961053, whole genome shotgun sequence.
ACCESSION   DASTIB010000235 DASTIB010000000
VERSION     DASTIB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961053"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..464
                     /locus_tag="VFP93_04085"
     CDS             117..464
                     /locus_tag="VFP93_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET9843815.1"
                     /translation="MQSGFDAIREKLLQKKETLQKRVEEIDHVLQRRSKPLDKDFAEQ
                     AVERENDEVLEALLQTSRNEIKQINRALNRIESGDYFVCARCGEDIPSGRLEAIPYTD
                     LCLECADHAQRRA"
     gene            579..848
                     /locus_tag="VFP93_04090"
     CDS             579..848
                     /locus_tag="VFP93_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843816.1"
                     /translation="MKRTIALTLISAFGLLGCATTHHHSHPEAVDLSSYEDGFRAGCE
                     SGIWYDLSEIKEKDFGRTMSDRAYMHGWDEGFSECRYNIYYNKNC"
     gene            complement(857..2086)
                     /locus_tag="VFP93_04095"
     CDS             complement(857..2086)
                     /locus_tag="VFP93_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9843817.1"
                     /translation="MNIASFNDKPLKIFIYLYRSYEKFTYDNCSFHAATLTFYTLFAL
                     VPFLGLMFGLAQSFGFESVLKTILKELLAAHPQVYENLLVFTNNLLLQTKEQIIAGVG
                     LLILIWSVYKVIWLIDHIFNDIWAIRFQKSPLYRVHQFAALLLLMPIILVILGGFLLQ
                     ILERITYYLPLIDSDISLSFPEFFRTKLVSVVLFTLLFGLSYWGLPAISVPIFPALIS
                     GFVGSLLFHASQLLYIHFQGWITEYGVVYGSFASVPLFMLWVYLSWMIYLFGAEMTHI
                     LESKITRSWEIKLNALPIRIQRVLALQIFEVIRERFSKGEPPLDAKMLAHTLKIPRAV
                     TRRILDLLIAAEVLTTTQRFNQAEPRYMPQKSIDLLDRDTIIKNLESSSLYGKPPETF
                     GQNIEQLLEPPPAKTEE"
     gene            2127..2933
                     /locus_tag="VFP93_04100"
     CDS             2127..2933
                     /locus_tag="VFP93_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843818.1"
                     /translation="MDTKKHYHILIATSCSNNTCHSQYTLSLANTIYHFANIKDVNLQ
                     VKLFNSEALLTKSRNICAAFVLSDTNITHLLFIDQNLQWEPAHVVRLLQHNKPIVAAT
                     YAKPKIYWERFDHDLFRKLNKLSDSTARQKSLQANLMNFQVKFADYKPPQQGLMEVVF
                     APVNFMLIQRDVFVKMSNQYPERKIAFDPSSEFKILNDYAYSLFDTEITDNNYWNSDA
                     SFCKRWIHLNEKIYLDLGIHLRYWGQYAFNGNILDLAPAKTFMPRDMKNE"
     gene            2926..3714
                     /locus_tag="VFP93_04105"
     CDS             2926..3714
                     /locus_tag="VFP93_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843819.1"
                     /translation="MSNEISQKILLATPCHQGMCHAQYASSLAETVLLLYKSNAITIQ
                     PKFLFEEPLISRARNILCAYFLAEPSFTHLLFIDADIQWKPSDILRLINHNKPIIAAT
                     YPKKSIHWERINTFFFKIANHIEDKKTLKNFILSYLLDFAVSFTDMQVTKDGLCEVNS
                     LANGFLLIQRQTLDLMIKSKMVHKTSFLDTEFAHLNTFAYNFFDYEHGENIYDAQEEY
                     FSYQWRKGGGKLYLDLSILLTRMGDLPFAGYIMDLLQKQDSKNA"
     gene            complement(3717..>4337)
                     /locus_tag="VFP93_04110"
     CDS             complement(3717..>4337)
                     /locus_tag="VFP93_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9843820.1"
                     /translation="KWVPNSMHIDPSDPSLKEFYETLIQNDMVLIVHTGQEAALDGSN
                     QELGNPLKLRFPLDLGVKVIMTHAASLGKCQDFESENTEYVPCFNLFIRLMEEEKYQK
                     KLYGDISATYFINRNLDHVIELLNRKDLHSRLLNGSDYPLIAVNILINLNKLLKAGLI
                     TETEKKALREIYLYNPFLFDFVLKRTLKHPKTKQRFLPIVFEDRKL"
BASE COUNT         1460 a          704 c          782 g         1391 t
ORIGIN      
        1 tgaaaatact taatatcgta attttataat aacaataaga ctaaattgta gtcacatttg
       61 acaagataaa tattaagttc taagataaac ttatatatta taatagtgga aggttcatgc
      121 aaagtggatt tgatgcaatt agagaaaagc tgcttcaaaa aaaggaaacg ttacaaaaac
      181 gcgttgaaga aattgatcat gttttgcaac ggcgcagcaa acctttagat aaagattttg
      241 cagagcaagc agttgagcgt gaaaatgacg aagttttaga agctttgctg caaacaagtc
      301 gtaatgagat taaacaaatt aatagagcgc ttaatcgcat agaatcaggt gattattttg
      361 tatgcgcgcg ctgcggcgaa gatattcctt ccggacgttt agaagctatt ccttatacag
      421 atttatgtct tgaatgcgct gatcatgcgc aaagaagagc atagtttgct gcttacgcaa
      481 tattaaaatt tcaaaatttc tgctcaattt ttctaaattt tctattcgga ccgccgatag
      541 aatggataga aaatttaatt gtcattgagg agaaattcat gaaaaggacg attgctttga
      601 ctcttatatc agcttttggc ttgctcggtt gtgcaacaac ccatcatcat tcgcatccag
      661 aggccgtcga tttaagttct tatgaagatg gttttagagc aggctgtgaa agtggcattt
      721 ggtatgattt atcggaaatc aaagaaaaag atttcggtag aactatgagc gatcgtgctt
      781 atatgcatgg atgggatgaa ggattttctg agtgccgtta taatatctat tacaataaaa
      841 actgctaatt gataaattat tcttcggttt ttgcaggagg aggctctaaa agttgttcaa
      901 tattttgtcc aaatgtttcc gggggctttc catagagaga agaactttct aagtttttta
      961 ttatggtatc acgatctaaa agatcgatgc ttttttgtgg catataccga ggctcggctt
     1021 ggttgaagcg ttgggtagtg gttaaaactt ctgcggcgat taaaagatcc aatattcttc
     1081 gtgtaacagc gcgaggaatt tttagcgtat gggctaacat ttttgcatct aaaggcggct
     1141 cgcctttgga aaatctttca cgtatcactt caaatatttg taacgcaagt actctttgaa
     1201 ttcgaatagg taaagcgttt aatttgattt cccagcttct tgtgatcttg ctttctagaa
     1261 tatgtgtcat ttccgcacca aaaagatata tcatccaact taaatatacc cagagcataa
     1321 agagaggtac gctggcgaaa cttccataaa caacaccgta ttccgtaatc catccctgga
     1381 aatgaatata caataattgt gaggcatgaa ataataaact tcccacaaaa ccggatatta
     1441 aagcggggaa aatgggcact gaaatggcgg gcaatcccca ataacttaaa ccaaatagca
     1501 aggtaaacaa aacgaccgat acaagttttg ttctaaaaaa ttcgggaaac gaaagcgaaa
     1561 tatcagaatc gatcaagggc aaataataag taatacgttc taatatttgc aataaaaatc
     1621 cacccagaat aacgaggatg ataggcatta agagcaataa tgcggcaaat tggtgcacac
     1681 gatagagtgg acttttttga aaacgaattg cccaaatgtc gttaaaaata tgatcaatta
     1741 accaaattac tttatatact gaccaaatta gtataagcaa acctacaccc gcaataattt
     1801 gctctttagt ttgcaataat aaattattcg taaaaacgag aagattttca tacacttggg
     1861 ggtgtgctgc aagaagttct tttaaaatgg tttttaaaac tgactcaaat ccgaagcttt
     1921 gtgccaaccc aaacattaaa cctaaaaagg gcactaaagc gaataaagta taaaatgtaa
     1981 gcgttgcagc atgaaaagag caattatcat aagtaaattt ttcatacgat cgatacagat
     2041 atataaatat tttgaggggc ttatcgttaa aacttgctat attcataccg aaacgtgagc
     2101 ctttatttta aaaataagtt gagataatgg ataccaaaaa gcattatcat attttgattg
     2161 ctacttcttg cagcaacaat acttgtcata gtcaatatac actttcattg gctaacacaa
     2221 tatatcattt tgcgaacatt aaagatgtta atttgcaagt aaagttattt aattcagaag
     2281 ctcttcttac taaatcaaga aatatctgcg cagcttttgt tctgagcgat acaaatatta
     2341 cccatctatt atttatcgat cagaatttac aatgggaacc ggcgcatgtt gtaaggttat
     2401 tgcaacataa taaacctatt gtggcagcta cttatgcgaa accaaaaatt tattgggaac
     2461 gttttgatca tgatttattc agaaaattaa ataaattgtc agactcaact gcgcgccaaa
     2521 aatcgttgca agctaatctt atgaattttc aagttaaatt tgctgattat aagccgcccc
     2581 aacagggact tatggaggtt gtttttgcac cggttaattt tatgctaatt caacgagatg
     2641 tgttcgtgaa aatgtcaaac caatatccgg agcgaaaaat tgcatttgat ccaagttctg
     2701 aattcaaaat cttaaatgat tatgcctata gtttattcga taccgaaatt acggataata
     2761 attattggaa ttctgatgcc agcttttgca aacgatggat ccatttaaat gaaaaaattt
     2821 atcttgattt aggcattcat cttcggtatt ggggacaata tgcttttaac ggcaatatct
     2881 tagatttagc accagctaaa acatttatgc ctcgagatat gaaaaatgag taacgaaatt
     2941 tcgcaaaaaa ttttattagc aaccccatgt catcagggga tgtgtcacgc ccaatatgca
     3001 tcatccttag cagaaaccgt gcttttatta tataaatcga atgctataac tattcaacca
     3061 aaatttttat ttgaggaacc tttaatttct cgagcgcgta atattttgtg tgcttatttc
     3121 ttagcagaac ctagctttac gcatttatta tttatcgatg ctgatatcca atggaaacca
     3181 agcgatattt tacggcttat aaaccataat aaaccaatta tcgcagcaac ttaccctaaa
     3241 aaatcgatac attgggaacg tataaatacg ttttttttta aaattgcaaa tcacattgaa
     3301 gataagaaaa cattaaagaa ttttattctc tcttatttgc ttgattttgc agtgagtttt
     3361 accgacatgc aagtaacaaa agatggatta tgcgaagtga atagtttagc caatggcttt
     3421 ctgttgattc aacgtcaaac attagatttg atgataaaat caaaaatggt tcataaaact
     3481 tcttttttag acacggaatt tgcacactta aatacttttg cttacaattt ttttgattat
     3541 gaacatggag aaaacattta tgatgcgcag gaggagtatt ttagctacca atggcgcaag
     3601 ggtggaggaa agctttactt agatttaagc attttgctaa cgcgtatggg ggatttacct
     3661 tttgcgggct atataatgga tttgcttcag aagcaagatt ctaagaacgc ataaaattac
     3721 aattttctat cttcaaaaac aataggcaaa aatctttgtt tggtttttgg atgttttaga
     3781 gttcttttta atacaaagtc gaaaagaaaa ggattataaa gataaatttc tcttaacgct
     3841 tttttttcag tttctgttat caagcctgct ttcaacaatt tgtttaaatt aatcaaaata
     3901 tttacggcaa ttagcggata gtcacttcca tttaaaagac gagaatgtag atctttgcga
     3961 tttagtaatt ctatgacatg atccaggttg cgatttataa aataggttgc agatatatcg
     4021 ccgtataatt ttttttggta tttttcttcc tccatgagtc ttataaataa attaaaacac
     4081 ggtacatatt cagtattctc actttcaaag tcttgacatt ttcctaaact agcggcatgt
     4141 gtcatgatta ctttgactcc taaatctaag ggaaaacgta atttgagggg attgcccaat
     4201 tcctgattgc ttccatcgag cgcagcttct tgacccgtgt gcacgattaa aaccatgtca
     4261 ttctgaatta aggtttcata gaattctttt aaagaaggat ctgatgggtc aatgtgcata
     4321 ctgttgggca cccattt
//
LOCUS       DASTIB010000236         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1405553, whole genome shotgun sequence.
ACCESSION   DASTIB010000236 DASTIB010000000
VERSION     DASTIB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405553"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2270
                     /locus_tag="VFP93_04115"
     CDS             <1..2270
                     /locus_tag="VFP93_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9843821.1"
                     /translation="EFSYNRFNPKYKSIYQVNSHITFLEGKALAWQGVPGPLSVKAKE
                     IPGVISVLRLVTYNENPTLSEKMLSNADQTRVFDDNSIVYADGNFLSFFNNKLLHGNR
                     DAFLPNAHAVALTQSTAKKLFGTEDAIGKIVRFDKNSFTVTAVLQDFPHNSSLQYDAI
                     FPVDYYAHQFTANGGNHGWKTIDEDLNAYSFRTFLQLNPNANTSNIETKLTHLFNATG
                     SGQAPTVFRLQNLGDLHLIGIDGNNSALQMVRIMALVAILILIIASINYVNLSTARAL
                     TRIREVSIKKIIGAKRSQLFLQFITETVLTFVFAVFVAITLIILLKPLYDRITGEQLT
                     LSLSNVSVLEILFLTLLVTIPISSIYPALLLSSFNPLGAVREKSFLGIKTSSFRKSLV
                     VLQFMISFILLVRTIIMGDQMAYIRNKDLGYDKNYVFTVSFPAEATKNTDAIENELLL
                     QKGILNVSFSSAEDITDVTEVSGDIVWDGKENNVPFMLWRVQADKNFIPTMKYQLIAG
                     TNFTGTSSDDNKYILNETAVKAMGLKPPYIGTKIGYGNADGEILGVIKDFNFKSLKDP
                     ITPLVIRTSGFKNILYVRTTGAGAQAAVKAAELQYKKYATHAPFSYGFLDKKFESHYQ
                     LQQRARTVFTTFAAIGILISSLGLFGLATYTAQVKTKEIGIRKVLGASVGSVIQLIGR
                     DFLKLVIIATVIATPLAYWAMNKWLQDFAYRIDISGWTFALAAIISLVIALVAISFQS
                     IKAGIANPVKSLRTE"
     gene            complement(2282..2356)
                     /locus_tag="VFP93_04120"
     tRNA            complement(2282..2356)
                     /locus_tag="VFP93_04120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2321..2323),aa:Arg,seq:cct)
BASE COUNT          765 a          449 c          482 g          734 t
ORIGIN      
        1 atgaatttag ctacaataga tttaacccga agtataaaag tatctaccag gtaaattcgc
       61 acattacctt tttagaagga aaagcacttg catggcaagg agtgccagga cctttatctg
      121 taaaggcaaa agaaattccg ggtgtaattt ctgttttaag actggtaact tataatgaaa
      181 atccaaccct ctccgaaaaa atgttgtcca atgctgatca gacaagagta tttgacgaca
      241 acagtattgt ttatgcagat ggtaattttt taagcttctt caacaacaaa ttgttgcatg
      301 gcaaccgtga tgcctttttg ccgaatgcgc acgctgtagc actcacacaa tcaacagcca
      361 aaaaattatt tggcacagaa gatgccattg gcaagatcgt tcggtttgat aaaaatagtt
      421 ttacggtaac cgccgtcttg caggactttc ctcataattc ttccctgcaa tacgatgcta
      481 tctttccggt agattattat gctcaccaat tcacagctaa tggcggtaat catggttgga
      541 aaacaatcga tgaagatcta aacgcttata gtttcagaac atttcttcag ctaaatccaa
      601 atgccaatac ttcaaatatt gagacaaaac ttacacattt atttaatgca acaggaagtg
      661 gtcaggctcc aacagttttc cgattacaga atttaggaga tcttcatctt ataggaattg
      721 acggaaataa ttctgcgtta caaatggtaa gaataatggc tcttgttgca atattaatat
      781 tgattattgc gagcattaac tatgtaaatc tttctacggc ccgggctctt acaagaatca
      841 gagaggtaag tataaagaaa atcatcggcg cgaaaagaag ccaactattt ttgcaattca
      901 taaccgagac ggtattgaca ttcgtatttg ctgtttttgt tgcaattact ttaatcattt
      961 tattgaagcc attgtatgat agaattacag gtgaacaatt aactctttct ctgtccaatg
     1021 tttctgtgtt agagatacta ttccttacgt tattagtgac aatacctatt tcaagtatct
     1081 atcccgcact gttgttgtcg tctttcaatc ccttaggcgc agtacgtgaa aaaagttttt
     1141 tgggtattaa aacgtcttca ttcagaaaat cattggtcgt tcttcaattt atgatctcgt
     1201 tcatactgtt ggttagaaca attataatgg gagatcaaat ggcgtatata agaaataaag
     1261 atttaggtta tgataagaac tatgttttta ctgtttcatt tcccgcagaa gcaacgaaaa
     1321 ataccgacgc tatagaaaat gaattattac tgcaaaaggg catattaaat gtttctttta
     1381 gcagcgcaga agacattaca gatgtgacag aggtgtcagg ggatattgtc tgggatggta
     1441 aagagaataa tgtaccgttt atgctttggc gtgtccaggc tgataaaaat ttcataccta
     1501 caatgaagta tcagcttatt gcaggtacaa attttaccgg aacgtcttca gatgataata
     1561 aatatatttt gaatgaaact gctgtaaaag ctatgggttt gaaacctcct tacatcggca
     1621 caaaaattgg ctatggaaat gcagatggag aaatattagg tgtaataaaa gattttaatt
     1681 ttaaatcgct aaaagatccc attacgcctt tggtaataag aacgtctggc ttcaaaaaca
     1741 ttttatatgt acgcacaaca ggtgcggggg cgcaggcggc cgtcaaagcc gcagaactgc
     1801 agtataaaaa atatgcaacc catgcacctt tctcttacgg ttttcttgat aagaaattcg
     1861 aatctcatta ccaattgcag cagcgtgcac gcactgtgtt tacaactttt gcagccatcg
     1921 gtattcttat ttccagtctt ggcttgttcg ggttggccac ttatactgcg caggtaaaga
     1981 ccaaagaaat cggcatcaga aaagtgctcg gcgcaagtgt cggcagcgtg atacaattga
     2041 ttggtagaga ttttttgaaa ttggtaatta tcgcgactgt aattgcaacg ccactggcct
     2101 actgggctat gaataagtgg ctgcaggatt ttgcttatcg catagacatc agcggatgga
     2161 cgtttgcttt agcagctatt atttccctgg taattgcttt agtagctatc agttttcaat
     2221 ccatcaaagc gggaattgcc aatcctgtaa agagtttaag aacggaatag atgttgcaca
     2281 ttggtagtcc cgacaggaat cgaacctgta tcaaaagttt aggaaacttc tattctatcc
     2341 attgaactac gggaccaggc ccgccttcac tatagcttcg gcggacaagg gcgccaaaaa
     2401 tacaaaagaa ttcctaatca acaggaacct
//
LOCUS       DASTIB010000237         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_447526, whole genome shotgun sequence.
ACCESSION   DASTIB010000237 DASTIB010000000
VERSION     DASTIB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447526"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFP93_04125"
     CDS             <1..631
                     /locus_tag="VFP93_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9843822.1"
                     /translation="THIKLAAVLEFIHSATLLHDDVVDQSQKRRGRYTANLIWGNAAP
                     ILVGDFLYSRAFQMIVSIKSMKILEILALATNVIAEGEVLQLAKRHDPHIDEETYLQI
                     IYAKTAKLFEVAAQLGALKQKNNFFEPCLQEYGKHVGIAFQLIDDVLDYSASPEIMGK
                     NVGDDLAEGKTTLPLLHALQKGTDAQKTFIQNTIRQGSCTILQLFKKLL"
     gene            960..>2419
                     /locus_tag="VFP93_04130"
     CDS             960..>2419
                     /locus_tag="VFP93_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843823.1"
                     /translation="MIKKEDIEAAREKLKKTTEKTPKKTLQSEILKGAPLRKTAPIQK
                     KVTNPIKESLSSRIKQFNGPVDETWEDTPDEVEKFEKEQNTSTPKPSSVLSNAQPQKD
                     VDSSSQENTKVESKTGKQDNEASIRGLEMEIALLNQRVHSKDRKKVKKLLKEKKMRLD
                     LLRNPLIEEKPKTPKLDSSRIKNLANKLKIRSNNLKPAENSSPSTTEKSTIIAKPDVK
                     DSGLKNPKKSRPIIKDTNVQDGEILRKDPPISSPDEPEQPLIHSITDKKTRRKEIKTL
                     ETKSDPDKSAQIRALEAQIQRLEMELKNGKGNPLTIQAELTKRNKEIDQLKPKPLSVE
                     RKAKNSNLISSLPTIVNPSMNKTIEKKVILNKEKSTMTAEPDMKDSVLANPQKSNPIL
                     KDITVKSKDIQEKIRLQMPRKTPQLRRDPPESKPDEPEQPLIYKKDPITHKKSIGVDT
                     TVKEPDRSVQIRAVEGQIRKLELQLKNGKADLSDIQ"
BASE COUNT          968 a          445 c          431 g          575 t
ORIGIN      
        1 aacccacatt aagctagctg ctgtattaga gtttattcac tcagctaccc tattacatga
       61 cgatgtggtg gatcaatctc aaaaaagacg tggtagatac acggcaaatc ttatttgggg
      121 caatgcagct cctattttgg tcggtgattt tctctattcg cgtgcttttc aaatgattgt
      181 atcgatcaaa tcaatgaaaa ttttagagat tttagcgtta gccaccaatg taatagcaga
      241 aggcgaagta ttacaattag ccaaacgcca tgacccccac atcgacgaag aaacctacct
      301 acaaatcatt tatgccaaaa ccgcaaaatt atttgaagtg gccgctcaat taggtgcgct
      361 aaaacaaaaa aacaattttt tcgagccttg tttgcaagaa tatggcaaac atgttggcat
      421 tgcatttcaa ctgattgatg atgtcttaga ttattcagct tctcccgaga taatgggtaa
      481 aaatgtaggc gatgatttag cggaaggaaa aacaactttg cctttactac acgcattaca
      541 aaaaggcaca gatgcgcaaa aaacttttat tcaaaatact attcgacaag gctcctgcac
      601 gattttacaa ttattcaaga agctattgta aacaccggtg ctatcgatta caccatgctc
      661 caagctgaaa aagaagtaga tcaagctttg caagtgttac acgatttgcc tgtaagcccc
      721 tatcgagatg ccttggcttc tttagcccaa tttgctattc atcgcaataa ttaacgggtg
      781 tagctcagcc tggtagagcg caattgcggg acgtagagat cgcaggatcg aagcctatca
      841 tttgaaaacc gcaaatttaa aaaaacatca aaatacgtac cattagtaac aactcgattg
      901 ggaacttagt ttggattctt gaggtctact tatatactgt tattggtaga cgtatactta
      961 tgatcaaaaa agaggatatt gaagctgcac gcgaaaaatt aaaaaaaaca acagaaaaaa
     1021 ctcccaaaaa aacgcttcaa tcagaaattc ttaaaggtgc acctttaaga aaaactgcac
     1081 ccatacaaaa aaaagtaaca aacccgatta aagaaagctt gagttctcga atcaaacaat
     1141 ttaatggacc ggtagacgaa acttgggaag atacaccaga tgaagtagag aaatttgaga
     1201 aagaacaaaa cactagcaca cctaaaccat caagcgtctt gagtaatgcg caacctcaaa
     1261 aagatgtaga ttctagtagc caagaaaata caaaagttga aagtaaaacc ggaaagcagg
     1321 ataacgaggc ttctataaga ggtcttgaaa tggaaattgc attacttaat caacgtgtac
     1381 attcaaaaga ccgaaaaaaa gttaaaaaat tacttaaaga aaaaaagatg cggcttgatt
     1441 tactgagaaa tccacttatt gaagagaaac caaaaacacc taaactagat tcgtctagaa
     1501 taaaaaattt ggcaaataaa ttaaagattc gcagtaataa tcttaagcca gcagaaaata
     1561 gttcgccatc gacaacggaa aaatcgacaa taattgctaa accggatgta aaagattcgg
     1621 gtttaaaaaa tccgaaaaaa agtaggccaa taataaaaga taccaacgtg caagatggag
     1681 aaattctgag aaaagatcca cccataagct cacccgatga acctgagcaa cctttgatac
     1741 atagtattac tgataaaaaa acacgtcgaa aagaaatcaa aacgcttgaa acaaaaagcg
     1801 atccggacaa atcggctcaa attagagcgc ttgaagcaca gatacaaagg ttagaaatgg
     1861 agctgaaaaa tggaaaagga aatcctctaa ctattcaagc ggaacttact aaaagaaata
     1921 aagaaattga tcaattaaaa cccaaacctc tttctgttga aagaaaggca aaaaattcaa
     1981 atttaatatc atcacttcct acaatagtaa atccttcaat gaataagaca attgagaaaa
     2041 aagttattct taacaaagaa aaatcaacaa tgacagccga accagatatg aaagattctg
     2101 ttttagcaaa tccgcaaaag agcaatccta ttttaaaaga tataaccgta aagagtaaag
     2161 atattcaaga aaaaattaga ctgcagatgc cacgtaaaac tccacaacta cggagagatc
     2221 cacctgaaag taaaccggat gaacctgagc aacctttgat atacaagaaa gatcctataa
     2281 cacacaaaaa gagtataggg gttgatacaa cagtaaagga acccgatcgt tcagttcaaa
     2341 tcagagctgt tgaaggccag ataagaaaac tcgaattgca gcttaaaaat ggaaaagcag
     2401 atctttcaga tattcaaga
//
LOCUS       DASTIB010000238         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_943524, whole genome shotgun sequence.
ACCESSION   DASTIB010000238 DASTIB010000000
VERSION     DASTIB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943524"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..935)
                     /locus_tag="VFP93_04135"
     CDS             complement(105..935)
                     /locus_tag="VFP93_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9843824.1"
                     /translation="MFFKFISGFLLILICIALQSCASGLKHTHRVLADAHHKGFKQEI
                     LSTSKFKLTTLHRITEKNAPLVVYLEGDGHSQSKKYRLSKDPTPHQPLALELALLDPR
                     PNVIYIARPCQYSIAEDAECNAMYWSTHKFAPEVINSTQEVINHFRSLHENKKIELVG
                     FSGGGGLAVMIAAQDPRIINVTTIAGDLDIELMQKHHNTTPLTGSKNPKRVATSINKI
                     PQRHFIGKKDVVVPLAVAESFVHQVGACAKIIALPKFEHHKGWKEAWPFLLTEPFPCG
                     "
     gene            1037..2083
                     /locus_tag="VFP93_04140"
     CDS             1037..2083
                     /locus_tag="VFP93_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HET9843825.1"
                     /translation="MIFSYPRVLTANLVIVGILSPVCGFCFDLQQFTNAQKFYFAGDE
                     QDQEENGDEPEYVPYIPKPYGAWFMTYGNHAKQEAQDATFGYSLGTLGFGLGLDWHYF
                     DGWIVGLGIRFDHTDLNSDNFVGTNIDFGSTRLFLYSRYNIAPHWFINGTAAYAFHTY
                     NTQRRGITFPFGVMEVTDIGGDIDGRQWFLELNTGYQWSCGNLQILPKIGMQYSNLSV
                     HEYSEFGVPGLNRFFPDHHLNSLKWNADLMLAYQNSFPNIQLLPFIHGKVFYDSEQDP
                     QELTANVLGGGSAFPTFGHLPPRTSYQVGAGFTLFGLHNMEVTMRYEYTFKHLYRDHA
                     ALIYIHHEWDANHK"
     gene            2096..>2324
                     /locus_tag="VFP93_04145"
     CDS             2096..>2324
                     /locus_tag="VFP93_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843826.1"
                     /translation="MTRMSNSLTYAVLSSSLFLSTLIYADITINNDVVTQQATSVSAN
                     ITIAPTGSVTVGGGDISVIVDTTNTLTLEPNT"
BASE COUNT          655 a          475 c          456 g          738 t
ORIGIN      
        1 aaatctaaaa aattctgcga cgctttttgg gtgagcgtac gcaataaatt ttgttcacga
       61 cgcttttcta gaaaatgttg atacgactcg taaatatgca taaactaacc acagggaaaa
      121 ggttcagtta ataaaaaagg ccaagcttct ttccagcctt tatgatgttc aaattttggt
      181 aaagcgatta tttttgcaca agcaccgact tgatgtacaa aactttcggc taccgcaagt
      241 ggcacaacaa catctttttt tccaataaaa tggcgctgtg gtattttgtt tatcgatgtg
      301 gcaacccttt tgggattttt tgatcccgtt aaaggcgtcg tgttatgatg tttttgcatc
      361 aattcaatat ctaaatcacc tgctatcgtg gttacattta taatcctggg atcttgagcc
      421 gcgatcatca ccgccaatcc tccaccaccg gaaaaaccca ccaattcgat ttttttattt
      481 tcatgtaagc ttctaaaatg gtttataact tcttgcgttg agtttattac ttctggcgca
      541 aatttatggg tcgaccaata cattgcgtta cactccgcat cctcagcgat tgaatactgg
      601 caaggccttg cgatataaat cacattgggc cgtggatcga gcaaagccaa ttctaaggct
      661 aaaggttgat ggggagttgg atccttagat aatcgatatt tcttgctttg actatgacca
      721 tccccttcta aatacacaac taagggagcg tttttttcgg taatacgatg cagggttgtt
      781 aacttaaatt tggaagtact caggatttct tgcttaaacc ccttatggtg cgcgtccgct
      841 aataccctat gtgtatgttt caatccagaa gcacaacttt gcagcgctat acagataagg
      901 atcaggagaa aacctgaaat gaatttaaaa aacataccac ggtagcctta caaacatagt
      961 atataataca ctggttctat tagaaattta aaatatgttt tttttcttac taagcattgt
     1021 tgcagggagt taattaatga ttttttctta tccccgcgtc ctcaccgcta atttggttat
     1081 agttggcatt ttaagccctg tttgtggatt ttgtttcgat cttcaacaat ttaccaacgc
     1141 ccaaaaattt tatttcgcgg gagatgaaca agatcaagaa gaaaatgggg atgagcccga
     1201 atacgtaccc tatattccca agccttatgg cgcttggttt atgacgtatg gcaatcatgc
     1261 gaagcaagaa gcacaagatg caacatttgg ctacagcttg ggcaccctag gatttggctt
     1321 aggactcgat tggcattatt ttgatggatg gattgtaggt cttggtattc gctttgatca
     1381 caccgatctc aattccgata attttgttgg caccaacatc gactttggca gcacacgttt
     1441 atttttatac tcgcgctaca atatagctcc ccattggttt atcaatggaa ctgctgctta
     1501 cgcattccac acatacaata cccaacgccg aggtattact tttccttttg gtgtcatgga
     1561 agttaccgat atcggtggcg atatcgatgg gcgacaatgg tttttagaac taaatacggg
     1621 ttatcaatgg tcttgcggaa atttgcaaat attgccaaaa ataggcatgc aatattctaa
     1681 tctttcagta catgaatatt cggaatttgg cgtgcctggc ttaaaccgct tttttccgga
     1741 tcatcatctc aactcgctta aatggaatgc ggatttgatg ctagcttatc aaaattcatt
     1801 ccccaatatt cagcttttgc cctttattca tggcaaagta ttttacgata gcgaacaaga
     1861 tccacaagag cttacggcca atgttttagg gggtggtagc gctttcccaa catttggcca
     1921 cctgccaccc agaactagct atcaagtggg ggctggtttt acgttatttg gtttgcataa
     1981 tatggaagtt acaatgcgct atgaatatac gtttaagcac ctatatcggg atcatgcagc
     2041 actcatttat attcaccacg aatgggatgc caatcataaa taaaagggat cgtgcatgac
     2101 aaggatgtcc aactctctca cttatgcagt gttatcttct tcgctttttt taagtacgtt
     2161 aatttatgct gacataacaa taaacaatga cgtggttacg cagcaagcta cttctgtctc
     2221 agcaaatatt accattgcac ctacaggctc tgttacggta ggagggggtg atatttctgt
     2281 catagtggat actacaaaca ctctaacttt agagccaaat actg
//
LOCUS       DASTIB010000239         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_588017, whole genome shotgun sequence.
ACCESSION   DASTIB010000239 DASTIB010000000
VERSION     DASTIB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588017"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFP93_04150"
     CDS             <1..679
                     /locus_tag="VFP93_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HET9843827.1"
                     /translation="EMDASIMDGRTLDAGAVSMIRTVKNPVVLSRIIMTKTKHVMFSG
                     YGALELAKRREVILEPESYFITDHQFDAFKKLAKPEEIQKAIEKKTHGTVGAVALDKK
                     GNLAAATSTGGTSHALPGRIGDSCIIGGGCYANNNTCAVSGTGQGEFLITGVVAHTIS
                     MLIELKRMPLQKACDEVIHERNKKQGEMGVIAVDKKGNVSFSFNTKIMRRGCIDAQGN
                     LFVSIYQ"
     gene            767..1327
                     /locus_tag="VFP93_04155"
     CDS             767..1327
                     /locus_tag="VFP93_04155"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET9843828.1"
                     /translation="MRRCSWVNLNNPLYVQYHDEEWGRPIHEEHQLFELLILEGAQAG
                     LSWETVLNKRVYYRKAFENFDPAKVARFTSKKVEKLLENPGLIRHRLKITSAISNAKF
                     FLAIQQEFGSFDNYIWSFVNFKPMVNRYRTTQEYPSKTPLSDTISKDLKKRGFKFVGS
                     TIIYAYLQAIGVVNDHVVSCSFKAQR"
     gene            1451..>2448
                     /locus_tag="VFP93_04160"
     CDS             1451..>2448
                     /locus_tag="VFP93_04160"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF039846.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET9843829.1"
                     /translation="MEIEAGPILSFREQKKKASKHFWEVSILVVVALAFKELFKIEGL
                     DAKKKVLYTDANSCFLRFDLSIEQGKTEKSVNYQIHNHTYAFTVPGTATSLHFGFASC
                     NGFSDLSLMKDIKNRSIMWERLQETHENKKYHILILGGDQVYADTVWSDVPSVREWKN
                     LPLERRRLESTNAAMEAELDNYYAKLYKRIWGYAPVQQTLASIPTLMMWDDHEIFDGF
                     GSYPDADQNCPVYQKIYEIARKYFLLFQQHISPDETEKRPGMLCSFSNISLGFKVNQY
                     LILLLDLRSQRTQKGIITHENWNAIFAWNETIKSGQYFHLLLVTSIPIVYPNFVFL"
BASE COUNT          796 a          414 c          481 g          757 t
ORIGIN      
        1 ggagatggat gcctcgatta tggatggtag aacgctcgat gccggagcgg tttctatgat
       61 acgcaccgtt aagaaccccg ttgtactcag tcgtataatt atgacaaaga caaaacatgt
      121 tatgttttcg gggtatggcg cgttagaatt ggccaaaaga cgcgaagtga tattagaacc
      181 tgaatcgtat tttattaccg atcaccaatt tgacgccttt aaaaaactgg caaaacccga
      241 agaaattcaa aaagcaattg agaaaaaaac gcatggcacg gttggagcag tagccctcga
      301 taaaaaaggg aatcttgctg cagcaacttc aaccggaggt acaagtcatg cattaccagg
      361 gcgcattggg gatagttgca ttattggtgg tggttgttat gctaacaata atacatgcgc
      421 agtttctgga acaggtcagg gggaattttt aattaccgga gttgttgcac ataccatctc
      481 aatgttgatt gaattaaaac gtatgcctct ccaaaaagcc tgcgatgaag taatccatga
      541 acgtaataaa aaacagggtg agatgggcgt aattgctgtt gataaaaaag gcaatgtaag
      601 tttttcgttt aatacgaaga tcatgcgacg aggatgtatt gatgcgcaag gaaatttatt
      661 tgttagtatt tatcaatagt ttctttaagg aaattcagta atacaatact tattatgctt
      721 ttctctttaa ttgcagtata attttcaaaa aaatattttc acaataatgc gtcgttgttc
      781 ttgggttaat ctcaacaatc ccttgtacgt tcagtatcat gatgaagagt ggggacgacc
      841 tatacatgag gagcaccaat tatttgagtt actcatttta gaaggtgcac aagctggctt
      901 aagttgggaa acagtcttaa acaaacgcgt ctattatcga aaagcgtttg aaaattttga
      961 tccggcaaaa gtagcaagat ttacctccaa aaaagtggaa aaattattag agaatcctgg
     1021 tttaattcgc catcgtttga aaattacttc tgcaatttca aatgctaaat tttttttagc
     1081 gattcaacaa gaatttggca gctttgataa ctatatttgg tcttttgtga atttcaaacc
     1141 tatggtaaac aggtatcgaa cgacgcaaga gtatcccagc aaaacaccgc taagtgatac
     1201 tatttccaaa gatttaaaaa aacgtggttt caaatttgtg ggaagtacta ttatatatgc
     1261 ctatttgcag gctataggtg tggtaaacga tcatgtcgta agttgcagtt ttaaagcgca
     1321 acgataattt ttttagcaat aattccaaac ttattcataa gttgtaatca taaattaaac
     1381 tataatttta agaacattgg caatcaagca tagtcaggtt accatagatt tttcacacaa
     1441 gctaagatct atggaaattg aagcaggccc catcttaagt tttcgcgaac aaaaaaagaa
     1501 agcatccaaa catttttggg aagtttctat tttagtagtg gtagcactgg ctttcaaaga
     1561 actatttaaa atagaaggtc ttgacgctaa aaaaaaggtg ctttataccg atgcgaattc
     1621 ttgcttttta cggtttgatc tgagtataga acaaggaaaa acggaaaaat ccgttaatta
     1681 tcagatccac aatcatacgt atgcttttac ggtgcctggt accgcaactt ctttgcattt
     1741 tggtttcgct tcttgtaacg gcttttccga tctttctctc atgaaagata taaaaaatcg
     1801 cagcattatg tgggaacgtt tacaggaaac acatgagaat aaaaagtatc atattctaat
     1861 cttaggaggc gatcaagttt atgccgatac tgtgtggagc gatgtaccct cggttcgtga
     1921 atggaaaaat ttacctctag aaagacgccg ccttgagtct accaacgctg ctatggaagc
     1981 agaattagat aattattatg ctaaacttta taaacgcatt tggggttatg cgcccgtgca
     2041 acaaacatta gcttccattc ctacgcttat gatgtgggat gatcatgaaa tttttgatgg
     2101 cttcggatct tacccagatg cagatcaaaa ttgtccggta tatcaaaaaa tatatgaaat
     2161 tgcgcgaaaa tattttttat tattccaaca acacatttct cctgatgaaa cagagaaacg
     2221 tcctggcatg ctctgctctt tttcgaatat ttctttaggt tttaaagtaa accaatacct
     2281 tattttactc ttagatctca gaagtcaacg aacgcaaaaa ggaattatta cgcacgagaa
     2341 ttggaacgct atatttgcgt ggaatgagac aatcaaatcg gggcaatatt ttcacttatt
     2401 gttagttact agtataccta ttgtttatcc taattttgtt tttttaca
//
LOCUS       DASTIB010000240         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_168659, whole genome shotgun sequence.
ACCESSION   DASTIB010000240 DASTIB010000000
VERSION     DASTIB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168659"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1208)
                     /locus_tag="VFP93_04165"
     CDS             complement(75..1208)
                     /locus_tag="VFP93_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9843830.1"
                     /translation="MDSKAVITGTGFYHPPFAITNHELVTSFNQYVDLFNTQNKDAIE
                     NGTKTPLEYSSAEFIEKASGIKNRYVIDKHGILDIHRMRPYFAQREDDALCLQAELAV
                     NAISPALQNAYKKNTDIDAIILSTSVLQRAYPNIAIEVQNALSATGFAFDMSVACSSA
                     TFGLSMANDLINSDQADLVVVVSPEISSAHLDFTLRDAHFIFGDAATAVIVENKHKVR
                     QKIAWDILSSHRTTEYSNHIRNNFGFINACELKNDESLAIKLFTQKGRKVFRDVVPKA
                     FEHILAHCEKHYLNSHDIKRYWLHQANRHMNDLIAKKLLNRDPTFEDAPLILDQYGNT
                     ASAGSIIAFHQYRDDLPIGTHGMICSFGAGYSIGSLLVKKINL"
     gene            complement(1239..1997)
                     /locus_tag="VFP93_04170"
     CDS             complement(1239..1997)
                     /locus_tag="VFP93_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9843831.1"
                     /translation="MSELIKTEIQEHILIITITRSEKLNALTQKMYHQLNEAFDELNQ
                     NPNVRVGLLHGEGDHFTAGNDLQDFLQAKNPEDLPHVVTFLQKLNHLDKPLVAALKGC
                     AIGIGTTLLSFCDLVYASKDAYFQLPFINLGIIPEAGSTEIFPRLIGYRKAAEYMFLG
                     EKFSAADALRYGLINAIIEEQDVVEFALQQAKKLAEKSPEALCTTKRLLRHTEKEIID
                     QAIERENHYLFKLLLSAYTKKNLQAFFANNLQKN"
     gene            complement(2029..2409)
                     /locus_tag="VFP93_04175"
     CDS             complement(2029..2409)
                     /locus_tag="VFP93_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843832.1"
                     /translation="MSVGDRLPNQVNLSVLKKLALFIQKEINILIDHYVKDSQKLMEK
                     LKVHITNADLKHIQRTVRDLRQSSQEIGAMQFSFLLLSLEIAVSEYRVREWQPAYNKI
                     EQYYNWVIDELATIRGTPSSILVK"
     gene            2591..>2891
                     /locus_tag="VFP93_04180"
     CDS             2591..>2891
                     /locus_tag="VFP93_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843833.1"
                     /translation="MNDLLQDKALLLHELKNWFETVRPTKLSPLQHSVKRDSVTLNDK
                     LILAIHFNSEDEIKRLLDIGADPNALSEKGFSVFQLALAYQREESVLDLLIEYGAK"
BASE COUNT          902 a          516 c          501 g          972 t
ORIGIN      
        1 cattttttcg agaggatcgt gttttttgca aaataataaa cgaagacaaa gccaaaaaaa
       61 tcgaacgtat aagttcataa gttaattttt ttaacgagta aacttccgat ggaataacca
      121 gccccaaaag aacatatcat accatgcgta cctataggta aatcatctcg gtattgatga
      181 aatgcaatga tgctgcctgc cgatgcggta ttaccatatt gatctaaaat aaggggagca
      241 tcttcaaagg taggatcacg attaagtaat tttttggcga ttaaatcgtt catatgtcga
      301 ttggcttgat gcagccaata gcgcttaatg tcgtggctat ttaaataatg cttttcacaa
      361 tgcgctaaaa tatgttcgaa tgctttggga acgacatcac gaaacacctt gcgccctttt
      421 tgggtaaaaa gcttaatagc tagggattca tcgtttttta attcacacgc attgataaat
      481 ccaaagttat tgcgaatgtg atttgaatat tccgtagttc tgtgcgaact aagtatgtcc
      541 caagcaatct tttgccgtac tttgtgttta ttttccacga tcacagcggt tgctgcatct
      601 ccgaaaataa agtgcgcatc acgcaaagta aaatctaaat gggccgatga aatttcagga
      661 cttaccacaa caaccaaatc ggcctgatcc gaattgatta aatcgttagc catgcttaaa
      721 ccaaacgtgg ccgaagagca cgcaacactc atatcaaacg caaaacccgt tgctgacaaa
      781 gcattttgta cttctattgc tatatttgga tatgcgcgtt gcaataccga agtggataaa
      841 ataattgcat caatatcggt atttttttta tatgcattct gtagcgctgg acttatcgcg
      901 tttactgcta gctctgcttg caagcacaaa gcatcatcct ctctttgcgc aaagtagggg
      961 cgcatcctat gaatatctaa aattccatgt ttatcaatta cgtaccgatt ttttatgccc
     1021 gaagcttttt caataaactc tgcagaggaa tattctagag gcgttttagt tccgttctct
     1081 atagcatctt tgttttgtgt attaaataaa tcaacatatt gattaaagga agtaacaagt
     1141 tcatgattcg taattgcaaa gggtgggtga tagaatccgg ttcccgtaat tacagccttt
     1201 gagtccatat tttatccaag ctcagtagtt acaaataatt aattcttttg caaattgtta
     1261 gcaaaaaaag cttgtaaatt ttttttagta taagctgaca atagtaattt aaataaatag
     1321 tggttttctc gttcgatagc ttgatcgata atttcttttt cagtatgccg taataatctt
     1381 tttgttgtac ataatgcttc gggagatttt tctgctaatt tttttgcttg ttgcagtgca
     1441 aattccacta catcttgctc ttcaataatg gcattgatca aaccatagcg taaagcatct
     1501 gcagctgaaa atttctcgcc caaaaacata tactctgcag cttttcggta accaattaag
     1561 cgtggaaaaa tttccgttga tcctgcttct ggaattatac ctaaattaat aaaaggcaat
     1621 tgaaaataag catctttgct agcataaact aaatcgcaaa atgataagag cgtggtacca
     1681 atgccaatag cacaaccctt taatgcggct accagtggtt tgtccaaatg attaagtttt
     1741 tgtaaaaaag ttacgacatg cggtaaatct tccggatttt ttgcttgtaa aaaatcttgc
     1801 aaatcatttc ccgcagtaaa atgatcgcct tctccgtgta gcaaacctac acgcacattt
     1861 ggattttgat taagctcatc aaatgcttca ttaagctgat gatacatttt ttgtgttaag
     1921 gcattaagct tttccgaacg tgtaatagta ataataagaa tatgttcttg tatttccgtt
     1981 ttaatgagct cagacatatt tcttccctaa aataatgacg taactttttt attttacaag
     2041 aatgcttgaa ggtgttcctc gaatggtagc tagctcatca ataacccaat tataatattg
     2101 ttcaatttta ttataagcag gttgccattc tcgaacgcga tattcactca ctgcaatttc
     2161 taaacttaac aataaaaaag aaaattgcat agcgcctatt tcttgactac tttgacgtag
     2221 atctcttacc gttctttgaa tatgcttgag atcggcgttc gttatgtgga cctttaattt
     2281 ttccattaat ttttgagaat ctttaacgta gtgatcaatc agaatattga tttctttttg
     2341 aataaaaagc gcaagttttt ttaatactga gaggttcact tgattaggca aacgatcccc
     2401 aacactcata gactacttcc taaaactcgt aaactatgga atggcagtat tatagtgcat
     2461 atacacaaat ttaacagtcc tgttttggga taagtggctg tataaagatg acttaaaatt
     2521 tggttactaa cttattattt attatcttat gatatactta cgcaatcgtt aacatgctag
     2581 tctaagtctt atgaatgatt tattgcaaga taaagctctt ttacttcatg agcttaaaaa
     2641 ttggtttgaa acggtaagac ctactaaatt gagtccttta caacatagtg taaaaagaga
     2701 ctctgtcacg ttaaatgata aattaattct tgctatccat tttaattctg aagacgagat
     2761 taaacgattg ttggatattg gtgcggatcc caatgcttta tccgaaaaag gttttagtgt
     2821 gtttcaattg gcactagcct atcaacgcga agaatcggtt cttgatttgc ttattgaata
     2881 tggtgcaaaa c
//
LOCUS       DASTIB010000241         4496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1249479, whole genome shotgun sequence.
ACCESSION   DASTIB010000241 DASTIB010000000
VERSION     DASTIB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249479"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2419)
                     /locus_tag="VFP93_04185"
     CDS             complement(<1..2419)
                     /locus_tag="VFP93_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005494416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET9843834.1"
                     /translation="MKRMLINATHGVRVALVDGQTLYDLDIEPTIQQPTVNNIYKGVI
                     NRIENSLGAAFINYGAERDGFLPIKEIAREYFKADYQYVQGERINISEVLKPGQEVIV
                     QVVKEQRGTKGAALRTFISLAGRYLVLMPNNPRAGGISRQIEGDEREELKTNLNQLHI
                     PEGMGIIVRTAGVGRSLEELQWDLEILLSQWKAILEAADTRPAPFLIYQESDVITRAL
                     RDNLRPEIEEILVDTPDAYQHARKHLEDTRPEFVSKLKFYDEADPLFNRYQVESQIES
                     AFRRVVGLPNGASIVIDPTEALTAIDINSAKATEGRDIEETALMTNIAAAKAIALQLR
                     LRDLGGLIVIDFIDMANPKHQKQVEYHLREELKKDRARVQMGRISRFGLLEMSRQRLQ
                     PSLGDVNLVPCPRCDGQGTIRGIESLTLSILRVIEEESMKPNTAQVRTQVPIEVATFL
                     MNEKREALIELERRHHVQILVITNQNITTPHYEVSRIRQDEASSRNIKLASYNLVKDI
                     EAPTYKSVQSNRTEEEPAIKYAPPSSPPPSPKPLQLQKESGLIRRLWDSLFGGAPKEE
                     VEKKFTPKPKLKDKSRSHRTKRDNRDIVDTRDKRDYVELTETREKHEEYDTVEAETQG
                     RKQVRRDNRRPHTSGQQNRRGSGNRAQPRNRQGRGRTSRPRSHEDYATEQSSYEHPRR
                     RSREMPSTTPASAPKISLEASTLEAQEVVIPTLDQGNASSSYFKENNRIEPEIRADHE
                     WSEKVADVIDEPPVSETMYEPKKPKDSRRDQTGFIKLTPEQAQSTRVEDTAQESSFHE
                     QPKLKQIV"
     gene            2789..3724
                     /locus_tag="VFP93_04190"
     CDS             2789..3724
                     /locus_tag="VFP93_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9843835.1"
                     /translation="MNNKVRKEVTYFEIKNENVDQRLDNFLLTKLKKITRPEVYKLIR
                     TGQVRVNSGRIKPSYRLQLGDKVRIPPFSLSTEKSKTPKQGVLDLIESRIAYENDSFI
                     VLNKPSGFAVHGGSGIQFGVIEAIRHLRPHAPFLELVHRLDRETSGCLLIAKTRKSLL
                     HLHKLLREETLTKIYYALVVGKWRGPKKVVMPLNKNQLKSGERVVKVDSEGKEASTKF
                     KILRQFEDCTLLEAMPITGRTHQIRVHAAFMGHPIIGDDKYGDKKINKEFSQRGIQRL
                     FLHAYSITIPQLEGKSETYICELDATLQNILEQLG"
     gene            3728..>4496
                     /locus_tag="VFP93_04195"
     CDS             3728..>4496
                     /locus_tag="VFP93_04195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET9843836.1"
                     /translation="MAEQYESEPSRWEQNVLQNILLTSVKEQRSGRRWGIFFKSAFLL
                     YLIFVLFLFWPSNRKKLEYRHPHTALVTIEGVISPTDEANADAIVTGLRNAFESGYSK
                     GIILRINSPGGSPVQSSYIYNEIRRLRARHPEKKLYAVISDVGGSGGYFVACAADEIY
                     ANESSIVGSIGVLMPNFGFTETIQKLGIEQRTITAGENKMFLDPFSPVVEQQQAFAQK
                     LVGNIHHHFIQAVKEGRGERLKEDPTIFSGLFWTGDQA"
BASE COUNT         1256 a          875 c          934 g         1431 t
ORIGIN      
        1 taacaatttg tttcagcttt ggttgctcat ggaatgaact ttcctgtgca gtatcttcga
       61 ctcgcgtgct ttgtgcttgc tcaggtgtga gcttgataaa acccgtttga tcgcgtctgc
      121 tatctttagg ctttttaggc tcatacatgg tttctgatac aggtggctcg tcaataacat
      181 ctgcaacctt ttcagaccat tcatggtcag cgcgtatttc gggctctatg cgattatttt
      241 ctttaaaata agaggaggac gcatttcctt ggtctagggt aggaatcacg acttcctgtg
      301 cttctaaagt agaagcttcc aaagatatct ttggcgcaga agcaggagtt gtgcttggca
      361 tctctctgga tcttctacgc ggatgttcat agctactttg ttccgttgca tagtcctcgt
      421 gcgatcttgg acgagaagtt ctacctctgc cctgacgatt acgcggttgg gcacgattgc
      481 cactaccacg gcgattttgt tgaccgcttg tgtgcggacg gcgattgtcg cgtcgtacct
      541 gttttctacc ctgagtttcc gcttctacag tgtcgtattc ttcatgcttt tctcgagtct
      601 ccgtgagctc aacataatcg cgtttatcac gggtgtctac gatatctcgg ttgtcgcgtt
      661 tggtgcgatg actacgagat ttatctttga gcttaggctt aggcgtgaat ttcttttcaa
      721 cttcttcttt tggagcccct ccaaaaagcg aatcccataa gcgtcgaatt aaaccagatt
      781 ctttttgcaa ttgaaggggt ttcggtgaag gtggtggaga ggaaggtgga gcatatttaa
      841 tagctggctc ttcttcggtt cgattggatt gcacactttt ataggttggc gcttctatat
      901 ctttaaccaa attataacta gcaagcttaa tattacgaga agaagcttca tcctgtctaa
      961 tgcgagatac ttcataatga ggcgttgtaa tattttgatt ggtaattact aaaatttgaa
     1021 catgatgacg tctttcaagt tcgattaagg cctcgcgttt ttcattcatc aaaaacgtgg
     1081 cgacttcgat aggaacttgt gttcttactt gcgccgtatt gggtttcatg ctttcttctt
     1141 cgattacacg aagaatggat agggttaagg attcaatacc tcgaatagta ccttgaccat
     1201 cacaacgagg gcatggcacc aaatttacat cgcctaagga tggttgtaag cgttggcgag
     1261 acatttctaa taaaccaaaa cggcttatgc gtcccatttg aacacgtgcc cgatcttttt
     1321 ttaactcttc gcgtaaatgg tattccactt gtttttgatg tttaggattt gccatatcga
     1381 taaaatcaat aacaattaat ccacccagat cacgtaaacg taattgcaac gcaatcgctt
     1441 ttgcggcggc aatattggtc ataagcgcag tctcttcgat gtctcttcct tcagtggctt
     1501 ttgcggaatt aatatcgatt gctgttaaag cttcggttgg atcaattaca atggacgcgc
     1561 cgttaggtaa acccactaca cgtctaaaag cagattcaat ttggctttca acttggtaac
     1621 gattaaacag gggatctgct tcatcataaa atttcaattt actgacaaat tccggacgtg
     1681 tatcttccaa atgctttcga gcatgctgat aagcgtctgg agtatctacg agaatttctt
     1741 cgatttcagg acgtaaattg tcacgtaagg ctcgcgttat aacatcgctt tcttgataaa
     1801 tgagaaatgg tgcaggcctt gtatccgctg cttctagtat tgccttccat tgactaagta
     1861 atatttccaa atcccattgt aattcttcta gacttctacc gacacctgca gttctaacga
     1921 taatgcccat accctcaggt atgtgaagtt gatttaaatt ggttttaagt tcttcacgtt
     1981 catcgccttc aatttgtcgt gaaataccac cagcacgcgg gttgttaggc attaatacta
     2041 aatatcgtcc cgctaaacta ataaaagtgc gcaatgcagc acctttggtg ccacgttgtt
     2101 ctttgacaac ttggacaata acttcttggc cgggctttaa tacttcacta atattaattc
     2161 tttcaccctg aacgtattga taatccgctt tgaaatattc gcgtgcaatt tctttgatgg
     2221 gtaaaaaacc gtctctttct gcaccataat taataaaagc agcgcctaaa ctattttcga
     2281 tgcggttgat tactcctttg tagatattat taacggtagg ttgttgaatg gtaggttcga
     2341 tatctaagtc gtataatgtt tgtccgtcta ctaaagcaac acgtacgcca tgtgtggcgt
     2401 ttattaacat tcttttcata ggttacaaaa ttctctattt tttttaagtt aaaaatgcgc
     2461 taacaatttg tcctaaaccg ccttttttgg cgtatttttg tactttctta tgactttaag
     2521 atcgcccgga aatggactta agagatgtgt tgttctaagc ccctacacgg ttactgtcaa
     2581 accatttagg atgatggcaa agtatgaggc tgcttgccgc atttctaaat gacgcagggc
     2641 aatccgcttt gtcttctaac taaattataa aaaaataggc acaaagagct aagcgcctgt
     2701 acaattggca ttttatcagc ttttccatag ctgaactagc gcattatatc ggtcaagccc
     2761 taccataagg aagccttttt gacacatcat gaacaataaa gttagaaaag aagttactta
     2821 tttcgaaata aaaaatgaaa acgtcgatca acgtttggat aacttccttt taacgaaatt
     2881 aaaaaaaatt accagaccgg aagtttacaa attaatacgt acggggcaag tgcgcgttaa
     2941 ttctggcagg ataaaaccta gttatcggtt acagctcggt gataaagttc gcataccgcc
     3001 attttcttta agcaccgaaa aaagtaagac acctaagcaa ggcgttttgg atttaataga
     3061 atcaagaatt gcttatgaga atgattcctt tattgtgtta aataaacctt ctgggttcgc
     3121 agtgcatggg ggaagtggca ttcaatttgg ggttattgaa gcaataagac atttacgacc
     3181 gcatgcgcct tttttagagt tagtgcatcg tttagatcgt gaaacctcag gttgtttgct
     3241 catcgcgaaa acacgcaaaa gtttattaca tttacacaaa ttgttacgtg aagagacttt
     3301 aactaaaatt tattatgcgt tagtagtggg aaaatggcgc ggtcccaaaa aagtagtgat
     3361 gccactaaat aaaaatcaac ttaaatcagg tgaacgtgtt gtgaaagtgg atagtgaagg
     3421 aaaagaggct tccaccaaat ttaaaatatt gagacaattt gaagattgta cactgttaga
     3481 agcaatgcct atcactgggc gcacccacca aattcgtgtg cacgcggctt ttatggggca
     3541 tcctattatt ggggatgata aatatggtga taaaaaaatt aataaagaat tttctcaacg
     3601 tgggatccaa agattatttt tacatgctta ttccattaca ataccgcaac ttgaaggtaa
     3661 atccgaaact tatatctgtg aattagacgc caccttacaa aatattttag aacaattagg
     3721 ataaagtatg gctgaacagt atgaaagtga accctctcga tgggagcaaa atgtgttaca
     3781 aaatattttg ctcacctcag ttaaagaaca acgatccgga agaaggtggg ggattttttt
     3841 taaaagtgct tttttattat atttaatttt tgttctcttt ttgttttggc cttctaacag
     3901 aaaaaaacta gaatatcgtc atccgcatac cgccttagtt accatcgaag gggttatttc
     3961 acctaccgat gaagcaaacg ccgatgctat tgtcactgga ttgcgcaatg catttgaatc
     4021 aggatacagt aaaggcatta ttttgcgcat aaattctccc ggcggtagtc ctgttcaatc
     4081 aagctatatc tacaatgaaa ttcgacgatt acgtgccaga catcctgaga aaaaactgta
     4141 tgcggtaatt tctgatgtgg gtggatcggg tggttacttt gtcgcatgtg ctgcggacga
     4201 aatttatgct aacgaatcaa gtattgtggg ctctattggg gtgcttatgc ccaattttgg
     4261 ttttacggaa accatacaga agcttggcat agaacaacgt acgatcaccg caggcgaaaa
     4321 taaaatgttt ttagatcctt tttctcctgt agtggagcaa cagcaagcgt ttgcacaaaa
     4381 attggtgggc aatatacatc atcactttat tcaggctgta aaagaaggta gaggagagcg
     4441 tttaaaagag gatcctacta ttttttccgg acttttttgg acgggtgatc aggcgc
//
LOCUS       DASTIB010000242         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1379021, whole genome shotgun sequence.
ACCESSION   DASTIB010000242 DASTIB010000000
VERSION     DASTIB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379021"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VFP93_04200"
     CDS             <1..1476
                     /locus_tag="VFP93_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET9843837.1"
                     /translation="VQRAGLTQNPLENAKLQFRVLAVPLSTLVFRTLASTTLSRAKQR
                     KCKNFFALGLVFWLYQRPMETTKKWIESKFKHQEDILQGNLLALEAGYRYAENSEMLE
                     EQPNIAKASLPQGTYRQITGNEALALGALLAQQQTGRSLLVAGYPITPASDILQWFAQ
                     HGIKTFQAEDEIAAIGAALGASFGGSLALTTTSGPGMDLKSEMLGLAVMVELPLVVID
                     VQRAGPSTGMPTKVEQSDLWMALYGRHGECPVPVIAASTPADCFHTILEVFKIAVTYM
                     TPVILLSDAYLANGAQPWLIPNLEELPKIATSIIPRITDFKPYKRNDNLARSWTTPGM
                     EGYEHRIGGLEKTDITGEISYDPVNHEKMVLLRKQKIENIAQNYSPTTIMGALEGSCL
                     VIGWGSTYGFILTAIQELQESNVSVSYVHLRHLYPLPLDLKPILSRFEKILVIEMNSG
                     QLRSILRAAYLLDIKGLNKIKGQPFTVTEIKQGILALLGDV"
     gene            1473..2477
                     /locus_tag="VFP93_04205"
     CDS             1473..2477
                     /locus_tag="VFP93_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET9843838.1"
                     /translation="MNPRSLTDKIILKRQVYASDQEVRWCPGCGDYAILATVQKTLPL
                     LNIPKENIVFISGIGCSSRFPYYLDTFGFHTIHGRAPTIATGLKITRPELTVFVVTGD
                     GDGLSIGSNHLLHLMRRNVNVVVLLFNNRIYGLTKGQYSPTSELGKQTKTSPLGTLEQ
                     NLEPLQFALGADCSFIARCLDIDSSGLQEILVAAVKHKGTSFIEILQNCNVFNDGAFK
                     EISDKLSRAQQSIFIKPGSPMIFGQGRDHALILEEGLLKTNTIDEAVKPYVHTTDMPK
                     NILCQLVAMKPPKFPLVTGILRQQERSVFEEELDKRFVTKPTPIVQNLLYQNSTWKVD
                     "
BASE COUNT          793 a          471 c          512 g          726 t
ORIGIN      
        1 gtgcaaagag caggtcttac ccaaaatcct ttagaaaatg caaagcttca atttcgtgtg
       61 ctcgctgtac ctttaagtac gcttgttttc agaactttag cgagcacaac tttatcgcga
      121 gccaagcaaa gaaaatgcaa aaatttcttt gccttaggtt tggttttttg gttgtatcaa
      181 agaccaatgg aaacaacaaa aaaatggatc gagtcaaaat ttaaacacca agaagacatt
      241 ttgcaaggga atcttttagc attagaagca ggttatcgct atgcggaaaa tagcgaaatg
      301 ttagaagagc agcctaatat agctaaagca tcgcttccac aaggcaccta tcgtcaaatc
      361 actggcaatg aagcattagc gttaggtgct ttattagctc aacaacaaac aggtcgatct
      421 ttgttagttg caggctatcc tattacacca gcatcggata ttttacagtg gtttgcacaa
      481 catggcataa aaacatttca agccgaagat gaaatagcag ccataggagc agcactcggg
      541 gcttctttcg gtggatctct tgcactaact actaccagtg gccctggtat ggatctaaaa
      601 tccgaaatgt taggattagc tgttatggtt gaattgcctt tagtggttat cgatgtgcaa
      661 agagcaggtc cttcaaccgg tatgccaacc aaagtagaac aatcggattt gtggatggct
      721 ttatatggtc gccacggaga atgtccagtt cctgtaatag cggcgagtac acccgccgac
      781 tgctttcaca cgattctcga agtttttaaa attgctgtga cctatatgac accggtaatt
      841 ttattgtcgg acgcatatct agcaaatggg gcccaacctt ggctcatacc taatcttgaa
      901 gaattgccta aaattgccac ttcgataata ccccgtatta cagattttaa accttataaa
      961 cgcaatgata atttagcacg ttcttggaca actcccggca tggaaggata tgaacataga
     1021 attggtggct tagagaaaac cgatataacc ggtgaaatca gttatgatcc cgttaatcac
     1081 gaaaaaatgg ttttgcttcg aaaacaaaaa atagaaaata tcgcgcagaa ttattctccg
     1141 actaccatta tgggagctct tgagggatcg tgtttagtga tagggtgggg gagcacctat
     1201 ggatttatat taacggccat acaagaatta caagaaagta atgtgtctgt cagttatgtt
     1261 cacttacgcc atctttatcc tttgccttta gatttaaaac ccattttatc aagatttgaa
     1321 aaaattttgg ttattgaaat gaacagcggc caactgcgaa gtatcttaag ggctgcgtat
     1381 cttttagata ttaaaggact taataaaata aaaggccagc catttaccgt aacggaaata
     1441 aaacaaggta ttctggcgtt attaggagat gtatgaaccc acgatccctc acggataaaa
     1501 taattcttaa acgacaagtt tatgcttccg atcaagaagt ccgctggtgt ccgggttgtg
     1561 gcgactatgc tattttggca acggttcaaa aaacattacc cttgttgaac ataccaaagg
     1621 aaaatatcgt atttatatcg ggcattgggt gttctagccg gtttccgtac tacttagata
     1681 cgtttggttt tcatacaatt catggtcgag cccccactat tgcaaccggt ttaaaaatta
     1741 caagacctga gcttacggta tttgtcgtga ccggagatgg cgatggttta agtattggaa
     1801 gtaaccattt gctacattta atgcgacgca atgtaaatgt tgttgtttta ctatttaata
     1861 atcgaattta tggtttaacc aagggccaat attctcctac ttccgaatta gggaagcaaa
     1921 caaaaacttc acccttgggt acacttgaac agaatttaga gccattacag tttgcattgg
     1981 gagctgattg tagttttata gcccgctgtc ttgatattga ttcctccggg ttgcaagaaa
     2041 ttttagtagc agccgtaaag cataaaggca cttcgtttat cgaaatcttg caaaattgta
     2101 atgtttttaa tgatggggca tttaaagaaa tatccgataa attatctcgc gcgcagcaaa
     2161 gtatttttat aaaaccagga tcacccatga tttttgggca gggacgggat cacgctctta
     2221 ttctagaaga agggttgttg aaaacaaata ctatagacga agccgttaaa ccatatgttc
     2281 atactacaga catgccaaaa aatatattgt gtcaattagt tgcgatgaaa ccaccgaaat
     2341 tccctttagt cacagggata ttgcgacaac aagaaagaag tgtttttgaa gaggaacttg
     2401 ataaacgttt cgtaactaaa ccgacgccca ttgtacagaa tttattatat caaaattcca
     2461 catggaaagt tgattaacat taaactctgg cacaagagaa tt
//
LOCUS       DASTIB010000243         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1379925, whole genome shotgun sequence.
ACCESSION   DASTIB010000243 DASTIB010000000
VERSION     DASTIB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379925"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VFP93_04210"
     CDS             complement(<1..1156)
                     /locus_tag="VFP93_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET9843839.1"
                     /translation="MTDQTATNKVSKTRTIFWPIFGIEHKKFLPMTFMIALILFIYTI
                     LRNMKDTLVVSSTGGSEVITALKLYAVLPSAIISFFIIAKLYNTFSREKVFYIIVTFF
                     IAFFGIFTLILYPNQAFFEPTNSSAWLREVLPQGKFYENFINMYQYWSFSLFYIFSEL
                     WGSIVASLLFWQFANSIVKVSEAKRFYAHFYLIANLATALSGVMGQYYSKLGKAMPTE
                     FERYAVTVQYTCWTVVVLGILVLFLYRYLNTVIVNDSRLVSAAEAIPEKKKLKLSMWE
                     SLKFIFSSKYLGLIALLVLSYGISINIVEVAWKHEIHMLYPAKNDYNHYMSMYSMING
                     LATFVVILIGSNVIRVLGWKVGALATPIMLGVTGLLFFYFMIFDETVTPMA"
     gene            complement(1366..2160)
                     /locus_tag="VFP93_04215"
     CDS             complement(1366..2160)
                     /locus_tag="VFP93_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9843840.1"
                     /translation="MSFIRYCLVSLFVLFASSCSKPRGDYEIYLGTISGPETELVEIA
                     KEVAKNKYNLHIKIVEFEDYNLPNVALVDGSIDANMFQHEPYLNLAMHYKGFDLAIVG
                     KTFVYPMGIYSKKYNSIDSLPHNATIAIPIDPSNGARALRLLANANLIEIPNVDDMHL
                     RPTNIVKNPKNLHIVELDAAQLPRVLTDVDAAIINTNFAIPAGLIPAKHAIFLEDKQS
                     PYANIVVVRKKDKDHPKFTLLMEALHSDEVATKARELFRDQAIQAW"
     gene            complement(2132..2773)
                     /locus_tag="VFP93_04220"
     CDS             complement(2132..2773)
                     /locus_tag="VFP93_04220"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HET9843841.1"
                     /translation="MWTEILKAIAETLEMVFISGFFCVIIGLPLGTLLYITRRGNLWE
                     NLPVNYLLGTLINATRSIPFIILMLALFPITRLIVGTSIGLYAALVPLSIAAIPFFAR
                     LVETSLNEVPQGLIEAVQSMGASKLQIIRKVLISEALPGITSSLTLTLVNLVGYSAMA
                     GAIGAGGLGKLAYHYGANRFDTMVMVSTVIIMIIIVQSIQSAGDYVVHKVLSR"
     gene            complement(2754..>3656)
                     /locus_tag="VFP93_04225"
     CDS             complement(2754..>3656)
                     /locus_tag="VFP93_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9843842.1"
                     /translation="IRCINRLEKPTSGSIVVNRYNITELKENDLRLARRQMGMIFQHF
                     NLLNRRNVFENISLPLELAGYSTNAIREKIEPLIELTELSKHVHKYPKQLSGGQKQRV
                     AIARALAPSPKVLLSDEATSALDPTSTQAIFELLRSINQELGITILLITHEMDVVKAI
                     CDRVAIIHDGSIIEQGSVLELFANPKTQHAKEFIKASSRMEAPTSLKEKMRDTPSAKC
                     GTILRISYHGESASQPIIGFLIQKYHITINIIQGNIEIIRGETMGIMIVEITGPKESV
                     QKSIEFLEKNHLRVEILGYVDRNS"
BASE COUNT         1206 a          681 c          674 g         1095 t
ORIGIN      
        1 cagccattgg cgtaactgtt tcatcgaata tcataaagta gaagaataac aagcctgtaa
       61 cgcctagcat aataggggtt gctaatgcac caactttcca tcccaaaaca cgaatcacgt
      121 tactaccaat gagaatgacc acaaaagtag caagaccatt gatcatagag tacatactca
      181 tgtaatgatt gtaatcattt ttggcgggat aaagcatatg aatttcatgc ttccaagcca
      241 cttctacaat attgatcgag attccataag aaagtaccaa tagagcaatt aaacctaaat
      301 atttagagct gaaaataaac tttaaacttt cccacatgct gagttttaat ttctttttct
      361 cagggatggc ttctgctgcc gataccaaac gcgagtcgtt aacaattacc gtgttcaaat
      421 accgatataa aaataatacc aaaataccta acaccacaac cgtccaacag gtgtattgta
      481 ccgttacggc ataacgttca aattctgtgg gcattgcttt gccaagcttg gagtaatatt
      541 gacccatcac accggataaa gcagtagcta aattcgcaat gagataaaag tgcgcataaa
      601 agcgttttgc ttctgatact tttacaatac tgttggcaaa ttgccagaat agaagcgacg
      661 caacgatact tccccataat tcagagaaaa tatagaaaag tgagaacgac caatattgat
      721 acatattgat aaagttttca taaaatttcc cttgcggtaa aacttctcgt aaccaagcac
      781 ttgagtttgt aggttcaaaa aaggcttgat taggatataa aattagcgta aatattccaa
      841 aaaacgcaat aaaaaaggtc acaataatgt aaaaaacctt ttctcttgag aaagtattat
      901 aaagcttagc tatgatgaaa aatgaaatta tcgccgaggg taaaacggca taaagtttta
      961 aagcggtaat aacttcagac ccaccagtgg aacttactac caaggtatct ttcatattcc
     1021 ttaaaatagt atatataaag aggattaaag caatcatgaa agtcatgggt aggaatttct
     1081 tgtgctcaat cccaaaaata ggccaaaaga ttgttcgggt tttgcttacc ttattggttg
     1141 ctgtttggtc agtcattgta tgatacctag cttatttcat attattatta gttaatcaaa
     1201 ttataaaata aatgtgaagt gacctattac tttagtcaca aaaaaaaagg gaaactcaga
     1261 aacgttcccc tgtttgggaa tgattctagc taaagaaata taaaatgcaa tacttttact
     1321 gagtatggat gataagtggt caagcaaaaa ggaccattag ctaagttacc aagcttgtat
     1381 tgcctgatct ctaaaaagct ctcgggcttt ggtcgcaact tcatcagagt gtaaagcttc
     1441 cattaataaa gtaaattttg gatgatcttt atcctttttt cttactacaa cgatattagc
     1501 ataaggcgat tgtttatctt ctagaaaaat ggcgtgttta gcaggaatta gacctgcggg
     1561 aatggcaaaa ttggtattta taatagcggc atccacatcc gttaagactc gtggcaattg
     1621 cgctgcatcc aactcgacaa tatgtaaatt ttttggattc tttacgatgt tggtagggcg
     1681 taaatgcata tcatcgacgt tgggaatttc aattaaattg gcattcgcga gtaaccgcaa
     1741 agcacgagct ccgttgctag gatcaatagg aatcgcaatg gtcgcattat ggggcaaaga
     1801 atcaatggag ttatatttct tagagtaaat acccatgggg tatacaaaag ttttacctac
     1861 gatagccaaa tcgaaacctt tgtaatgcat agctaaatta agataaggtt catgctggaa
     1921 catattagca tcgatagagc catcgactaa tgcaacattc ggtaaattgt aatcttcaaa
     1981 ttctactatt tttatgtgta aattatattt gttctttgct acttcttttg caatttctac
     2041 taattcagtt tctggacccg aaatggttcc taaatatatt tcataatcgc cacgaggttt
     2101 tgaacaagaa cttgcaaaca atacaaataa actaacgaga caatacctta tgaacgacat
     2161 aatcacctgc actttgaata ctttggacaa tgatgatcat aatgattacc gttgaaacca
     2221 tcaccattgt atcgaaacgg tttgccccgt aatggtaggc aagctttcct aaaccgccag
     2281 cgcctatagc tccagccatg gcagaatatc ctactaagtt aactaaagtc aatgttagac
     2341 tgctggtaat tcccggtaat gcttcggaaa ttaacacctt tcggataatt tgtaatttgg
     2401 aagctcccat cgattgcact gcttcaataa ggccttgcgg cacctcattt agcgaagttt
     2461 ctacgagacg tgcaaaaaaa gggatagctg caatagataa agggacaagc gcggcataca
     2521 ggccaataga cgttccaaca attaatcgtg ttatgggaaa taatgcaagc attaaaataa
     2581 taaaaggaat agagcgcgtt gcattgatga gcgttcctaa taaataatta acaggaagat
     2641 tttcccagag attgccccgg cgcgtaatgt acagtaaagt acccaaaggc aaccctataa
     2701 taacgcaaaa aaaacccgaa atgaaaacca tttcaagggt ttcggcaatt gctttaagaa
     2761 tttctgtcca cataccctaa tatctcaaca cgtaaatggt tcttttctaa aaattcaata
     2821 cttttttgga cactttcttt ggggccagtg atttcaacta tcataatacc catggtttca
     2881 ccgcgaatta tttctatatt tccttgaata atgttaatcg taatatgata tttttggata
     2941 agaaatccaa taattggttg agaagccgac tcgccatgat aagaaattct taaaatagtt
     3001 ccacatttag cgcttggggt atcacgcatt ttttctttta atgaggtagg agcttccata
     3061 cgcgatgagg ctttaataaa ttctttggcg tgctgcgtct taggattggc aaatagctcc
     3121 aatacggagc cttgctctat tatacttcca tcatgaataa tcgcaacgcg atcacaaatg
     3181 gcttttacca catccatttc gtgcgtaata agtaaaatcg ttatgcctaa ttcttgatta
     3241 atcgatcgta gcaactcaaa aattgcttgg gtggatgtgg gatcaagtgc ggaagttgct
     3301 tcatcactta ataagacttt tggagaaggg gctagcgctc ttgcaatagc cacacgttgc
     3361 ttttgaccac cacttaattg tttgggatat ttatgaacat gcttggaaag ctcagttaat
     3421 tctattagcg gttcaatttt ttcccgaatt gcattggtgg aatatcctgc caactctagg
     3481 ggtaaactaa tattttcaaa tacattacga cgattgagta aattaaagtg ttgaaagatc
     3541 atgcccattt gacgacgcgc aagtcggaga tcattctctt taagctctgt gatgttatat
     3601 cggtttacaa cgatcgatcc gctagttggt ttttctaaac ggtttataca gcgaat
//
LOCUS       DASTIB010000244         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_936934, whole genome shotgun sequence.
ACCESSION   DASTIB010000244 DASTIB010000000
VERSION     DASTIB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936934"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1890)
                     /locus_tag="VFP93_04230"
     CDS             complement(34..1890)
                     /locus_tag="VFP93_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843843.1"
                     /translation="MKYWPETTLSFLLFFTFILYWPGLYGPFLLDDLQTIEMARVQVF
                     HWNDWLSKIVSNDAGPLRRPISILSFILTNFFFGDSPFAYKCINVIIHLLNSVLVYVL
                     LQNLLPYFSKNSLQQNTWISIAITAFWTIHPLQVSTVLYTVQRMMLLSSFFFLLSLLC
                     YVKFRLELGSKKTKIFLFFSMLTSWILAIFSKENALLLPGILLAFEMLCYQTPQRSKV
                     FILGSSIICFVLVLGGLAYYVNQYPTYALSFAEKGFTLPHYIFLQMQVLVFYLKLIVT
                     PSLGLMGLYHDDFQLTQGTPIWLSVGILLLLCVIAIGFLKRYALLSFFIFWFFMHHSL
                     ESSILPLEPVFEHRNYLASLGIIAAPILFLVWKFQTKHARIALQVFGGSGILLIASLT
                     WARVESFSSSEKFLIEAHYAHPLSSRTHIEWANFLFAQNEFGMALMALNRAHQLNPHN
                     SGPLVHQFLTHCFIQAPSDTIYQESVTALSETSITPYTILVLDALVQNHFNHVCPNIP
                     LSKSLTLIHAAIKNPLLEHKPSYRATLFHLQAGLLLLNQEPDKAIFALEQSFQLNPNR
                     VQPLLEKIQLLINQGEYLRAKQALNFVKQNFDIFGDTLEKVQFLQHQITQKI"
BASE COUNT          705 a          350 c          386 g          578 t
ORIGIN      
        1 tttataggaa cattatattg tattgcagca aaattaaatt ttttgggtta tctggtgttg
       61 taagaactga accttttcta aggtgtcccc aaaaatatca aaattttgct tcacaaaatt
      121 gagtgcttgt tttgcccgta ggtactcacc ttgatttatt aataattgta ttttttccaa
      181 taagggctga accctatttg ggtttaattg aaaagattgt tctaaagcaa aaatcgcttt
      241 gtctggttct tgatttaata ataacaaccc agcttgcaga tgaaataatg tggcccgata
      301 acttggctta tgctcgagta aaggattttt gatagcagca tgtattaacg tgagtgattt
      361 acttaatgga atattggggc aaacatggtt aaagtgattt tgtactaaag catccaatac
      421 taaaatagtg taaggcgtaa tacttgtttc ggatagtgcc gttacgcttt cttggtagat
      481 cgtatcagaa ggtgcttgta taaaacaatg cgttaaaaat tggtgcacga ggggaccaga
      541 attatgcggg tttaattgat gtgccctatt taatgccatc aaagccatgc caaattcatt
      601 ttgggcaaaa agaaaattag cccattcaat atgggtgcga gaggaaagcg gatgcgcata
      661 atgcgcttca attaaaaatt tctcgctgga agaaaaactt tccacgcgtg cccaagttaa
      721 gctggcaatt aataaaatgc cgctaccgcc aaatacctgc aaagcaatgc gtgcgtgttt
      781 cgtctggaat ttccaaacta ggaagagaat gggggcggcg attattccaa gacttgctaa
      841 atagttacga tgctcgaata caggttccaa aggtaaaata ctggattcta gactgtgatg
      901 cataaaaaac caaaaaataa aaaaactaag taaagcgtat cttttcagaa atccaatagc
      961 tattacacat aaaagcagga gaatacctac gcttagccaa attggggtac cctgtgtgag
     1021 ttgaaaatcg tcatggtata aacccattaa tcccaaactg ggcgttacaa tcaattttaa
     1081 atagaatacc aatacttgca tttgtaaaaa aatatagtgt ggtaacgtaa aacctttttc
     1141 tgcaaaacta agcgcataag tagggtattg gtttacgtaa tacgctaaac cacctaagac
     1201 tagaacaaag caaataatgc ttgacccaag tataaaaact tttgaacgct gcggtgtttg
     1261 gtagcagagc atttcaaatg ccaaaagaat tcctggtaga agcagggcat tttctttcga
     1321 aaaaattgcc aatatccaac ttgtgagcat agaaaaaaaa agaaaaattt tggtcttttt
     1381 gctgcccaat tccaatcgaa atttcacata acataaaaga ctcaataaaa agaaaaaact
     1441 agacaacaac atcatgcgtt gtactgtgta gagcacagta ctgacctgca atgggtggat
     1501 tgtccaaaaa gccgtaatcg caatacttat ccatgtattt tgctgaagtg aatttttaga
     1561 aaaatacggt aaaagatttt gtaaaagcac gtataccaat acactattca agagatgtat
     1621 tattacattg atacatttat aagcaaatgg actatcccca aaaaagaaat tcgttaaaat
     1681 aaaacttaga atactaatag gtctacgtaa ggggcccgca tcattggaaa ctattttcga
     1741 taaccaatcg ttccaatgaa acacttgcac tcttgccatc tcgatcgttt gtagatcgtc
     1801 taataaaaag ggtccataga gacccggcca gtataaaata aatgtgaaaa acaaaagaaa
     1861 acttaaagta gtttctggcc aatatttcat gcaaatcctt caataaaaaa aggaggtata
     1921 aacctccttt tttggacatt tttttaacgg caggatgcgg gggcgtattg ggtagcaccc
     1981 gttgcagtac aagtccactg caccgcaccg ttttgaaaa
//
LOCUS       DASTIB010000245         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_599802, whole genome shotgun sequence.
ACCESSION   DASTIB010000245 DASTIB010000000
VERSION     DASTIB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599802"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..551
                     /locus_tag="VFP93_04235"
     CDS             138..551
                     /locus_tag="VFP93_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9843844.1"
                     /translation="MSILVVEDNPNDVFILQKCFELLPSPGKLIHQRTAMDALSYLQE
                     LHDKNALDEIQLILLDLNLPGIDGRGFLKEMKTNDRFKSIPVIILSTSNNYSDVAFCY
                     AQGANCYLQKPFDFDDMKILAKRIDEFWLHTAILP"
     gene            524..1471
                     /locus_tag="VFP93_04240"
     CDS             524..1471
                     /locus_tag="VFP93_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HET9843845.1"
                     /translation="MVTHRDTTLEDRMAHTLKTILLVDDNEDDLFFYKKHLSDYTDIK
                     EILEAKNIEDALSIIEHKNISCLLVDYMLRGESGLDLVKTLKHSDRSKDIPIILLTGQ
                     GNENVAIETMHLGVQDYMIKQNITKETLYAAIIKSIDKLHQEIKVEYLAFHDPLTGAY
                     NRAYFCDYAQSAIERAFRYGYEIALLFIDLDHFKPINDKWGHDIGDEVLRVVANRLKD
                     SIRKMDILARMGGDEFVILIENVKSYQVHYISQKILTAFHTPSILDGHSVPIQISLGF
                     SMLPSQAKTLDELLKQGDLALYSVKKQEPGFYHRFQGKR"
     gene            1481..3775
                     /locus_tag="VFP93_04245"
     CDS             1481..3775
                     /locus_tag="VFP93_04245"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET9843846.1"
                     /translation="MKELHNFLRTFQSIIRYLLRASMLFFIYVLLAEISLKFTVPPGF
                     STSMWLPSGFALSFLFLWGVHYLPSFFLGHFFSNILLLDAEQSFTNIYILSFFLAAGG
                     SIQCLIGYLIAHKKIKLPFYNNDWRAITFLLIIAGPLVCTTSPTVGTLSLYFMGLVED
                     KQLWINWLTWWVGDSLGVLVMTPFLLFILTHNKNFPWKKRFFIIFPLLVFFVCFLGMY
                     LGSQKWFISHQEQRQDRYLMAINSAMQSQLDSYLFALFSLANYFKAEDEVTQNAFSVF
                     ARELMIRYPVIESLEWAPIVTHQQREAYERTLALRGIENPIIRERNEQRLLKPAQNRE
                     VYSPITYIESNQFKNMIGYDLFSEKQHEKIYEDALKWKKPVTKDRIVTHKHPSGVFVV
                     LHPVFKKDVAVGFVIAAFATSKIFDQFLPLLSSTNITFKVTQNYETIFDNTGSDAYQN
                     VIYRSLLLNVANRVWKVNYHYTLVSLLGSELWIYSTLLFLGLISICFLSIILSYTLVN
                     YTLAENLVRELRLRTNALFRSNQALEDFAYIASHDLKEPLRGVSNFSQLLKEEYESIL
                     DSEALSYLERIKQLCLKMNNLITDLLNYSKLTYKFNPTELVDFNQLIHETFHYVHSLY
                     PQETIKFSTVLLPKIVCDKIQMQQVFYNLMVNSIKYNDNRTKKITIGYFYQDFPTFFV
                     KDNGIGIEEQHKEHVFKMFKRLHVSDYYGEGTGAGLSYVKRVIEMHGGKIWIENNKGS
                     GITIYFTLQQEYYARQANKNTSSF"
     gene            3822..>4471
                     /locus_tag="VFP93_04250"
     CDS             3822..>4471
                     /locus_tag="VFP93_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9843847.1"
                     /translation="MFELFNLFNTDPYPARWHCGNWTASVGWTHILSDVAIGLAYIGI
                     PSAIIYYAHKLKMLELNYILILFSIFIFSCGTTHIMEAIIFWWPGYNLAAVLKLLTAI
                     VSWATLIAIIILLPESINLLKAKAQAEKLQHDLDKMELRHKGLEEFAHIASHDLQEPL
                     RNIHSLVEMAQNVISNPDELRTYFESILNSSRKMHALIHDLLDFAKISKEQFAFKE"
BASE COUNT         1404 a          789 c          786 g         1492 t
ORIGIN      
        1 tctaccgttt atggcatcaa aaagatgaaa cactcgaaag gttaaaatcc ttctaacttc
       61 tataaactaa agcttctata attaaagagg gaaagactcc ggtttcccat tgcattgatt
      121 tggtatggga gttaaggatg tcaattttgg tagtggaaga taatccgaac gatgttttta
      181 tcttgcaaaa gtgttttgaa cttttgccct ctcctggaaa actaatacac caaagaaccg
      241 ctatggacgc actcagttat cttcaagaat tacacgacaa aaatgcactg gatgaaattc
      301 aactcattct actggatcta aacttacctg gtattgatgg acgtggattt ttaaaagaaa
      361 tgaaaaccaa tgaccgtttt aaaagtatcc cggttatcat attatcgact tcgaataact
      421 attctgacgt agcgttttgc tatgcccagg gcgcgaattg ctatttgcaa aagccctttg
      481 atttcgatga tatgaaaata cttgcgaaac gcattgatga gttttggtta cacaccgcga
      541 tactacctta gaggacagaa tggcgcatac gttaaaaaca atattattgg tcgatgataa
      601 cgaggacgat cttttctttt ataagaaaca tttatccgat tatacagata ttaaagaaat
      661 attagaagca aaaaatattg aagatgcatt gagtattatt gagcataaaa atatttcttg
      721 tttattagtt gattatatgt tgcgagggga atccggttta gacttagtaa agacattaaa
      781 acatagtgat cgatcgaaag atattcctat tatacttctt acgggtcaag gtaacgaaaa
      841 tgttgctatc gagaccatgc acttaggtgt tcaagattat atgataaaac aaaatataac
      901 gaaagaaaca ctgtatgctg cgattataaa atccatcgat aaacttcatc aagaaattaa
      961 agtagaatat ttggctttcc atgatccctt aactggtgct tataatcgtg cctatttttg
     1021 tgattacgcg caaagtgcaa ttgaacgcgc tttccgctat ggctatgaaa tagcattact
     1081 atttattgat ctggatcact tcaaaccaat taacgataaa tggggtcatg atataggaga
     1141 tgaagtttta cgcgttgtgg caaatcgatt aaaagacagt attcgcaaaa tggatatttt
     1201 agctcggatg ggtggggatg aattcgtaat ccttattgag aatgtaaaat cctatcaagt
     1261 ccattatatt tcgcaaaaaa tcttgaccgc atttcacacg ccttccatat tagatggaca
     1321 ctccgtccct attcaaatat ccctgggttt ctctatgctc ccttcgcaag caaaaacatt
     1381 ggatgaatta ttaaagcaag gtgatcttgc gctgtattcg gtcaaaaagc aggaacctgg
     1441 gttctatcat agatttcaag gaaaaagata gaaatcatgt atgaaagagc tgcataattt
     1501 tctaaggact tttcagtcta tcattcgcta tcttcttcgg gcttccatgc ttttctttat
     1561 ctatgtattg ttagcggaaa tttctttaaa atttactgta cctcccggtt tttctacctc
     1621 catgtggctt ccttctggtt ttgccttatc ttttttattt ttgtggggcg ttcactattt
     1681 accgtcgttt ttcttgggac attttttttc gaacattctt ttattagatg ctgaacaaag
     1741 ctttactaat atttatattc tatctttttt tctagctgcc ggcggatcta ttcaatgttt
     1801 aataggttat ttaattgcgc ataaaaaaat aaaattacct ttctataata atgattggag
     1861 agccatcact tttttactca ttatcgcggg gcccttggtt tgcacgacct ctcccaccgt
     1921 aggaacatta tccttatatt ttatgggact cgtagaagat aagcaattat ggataaattg
     1981 gcttacttgg tgggtaggtg attctctggg tgttctcgta atgactccat ttcttttatt
     2041 tattttaacg cataataaaa attttccgtg gaagaaaaga ttttttatta tatttccttt
     2101 gttagtgttt tttgtctgtt ttttagggat gtatttgggt tcacaaaagt ggtttatttc
     2161 tcatcaagag caacgccaag accgctattt aatggctata aatagtgcga tgcaatcaca
     2221 gcttgatagt tacttattcg ctcttttctc tctggctaat tactttaaag cggaagatga
     2281 agtgacacaa aatgcgttta gcgtgtttgc tcgagaactt atgatccgat atcctgtcat
     2341 agagtcttta gaatgggcac ccattgttac ccatcaacag cgagaggctt atgaacgtac
     2401 actcgcactc cgtggcattg aaaatcccat aattcgtgaa cgcaatgaac aaagactgct
     2461 caaacctgct caaaatcgtg aagtttattc acccattacc tatatagaat ccaaccaatt
     2521 taaaaatatg ataggatatg atttattctc ggaaaaacaa catgaaaaaa tatacgaaga
     2581 tgcacttaaa tggaaaaaac ccgttacgaa agatcgtatc gttacgcata aacaccccag
     2641 cggcgtcttc gttgttttac atcctgtatt caaaaaagac gtagcggtgg ggttcgttat
     2701 cgccgctttt gcaacttcta aaatattcga tcaattctta cccttgcttt caagtaccaa
     2761 cattactttt aaagtaacac aaaattatga gactattttt gataataccg gtagtgatgc
     2821 ttaccaaaat gttatttatc gttcactttt actcaatgtt gcgaataggg tgtggaaagt
     2881 gaactatcat tatactttgg tatctttatt aggaagtgag ctttggatct atagcacttt
     2941 actattttta ggccttattt ccatttgttt tttatccatt attttatcgt atacgttagt
     3001 caattacaca ttagccgaaa atttggtgcg cgagttaaga ttgcgaacca acgctctttt
     3061 tcgttcgaac caagcgttgg aagattttgc ctatatcgca tcgcatgatt taaaagaacc
     3121 actgcgcggg gtgagcaatt tttcacagct tcttaaagaa gaatatgaaa gtattttgga
     3181 ttccgaagct ttatcctact tagaacgtat caagcaatta tgtttaaaaa tgaataatct
     3241 tattacagat ttgctcaatt attcaaaatt aacttataaa ttcaatccta cggaattggt
     3301 agatttcaat caattaattc atgaaacttt tcattacgtg cattcgttat atcctcaaga
     3361 aaccataaaa ttttcaacag tactcttacc taaaattgtt tgtgacaaaa ttcaaatgca
     3421 acaagtattt tataatctga tggttaattc cattaaatac aatgataatc gcacaaaaaa
     3481 aataacgata ggatactttt atcaagactt tcccactttt tttgttaaag ataatggcat
     3541 tggaattgaa gagcaacaca aagaacacgt tttcaaaatg tttaaacgct tgcatgtttc
     3601 ggattactat ggagaaggta caggagcagg attaagctat gtaaaacgtg ttatagaaat
     3661 gcatggtgga aaaatttgga ttgaaaataa caaaggctcc gggatcacca tctattttac
     3721 attgcaacaa gaatactacg ctagacaagc taataaaaat acctcatcat tctaaaaaat
     3781 cacacgtgtc ccccttcttt gcgcaaaagg aatgctttac tatgtttgag cttttcaatc
     3841 tatttaatac ggatccatac cctgcgcgat ggcattgtgg taattggact gcctcagtag
     3901 gctggacaca tatcttatcg gatgtagcta ttggtttagc ttatataggc attccttcag
     3961 caataattta ttatgcacac aaactgaaaa tgcttgaact caattatata ttaattcttt
     4021 tttctatttt tattttctcc tgtggcacca cccatattat ggaagcgatt attttttggt
     4081 ggccaggcta taacttagca gctgttttga agttattaac cgctattgtt tcttgggcta
     4141 cgttgatcgc cattattata ttgctaccgg aatctattaa tttattaaaa gcgaaagcac
     4201 aagcagaaaa gttacagcat gacttagata aaatggaatt gcgccataag ggtttagaag
     4261 agtttgcgca catagcctct cacgatttgc aagaaccttt aagaaatatc cacagcttag
     4321 tggaaatggc acaaaatgtg atctccaatc cagacgaatt gcgcacctac tttgagtcca
     4381 ttttaaacag ttcgcgaaaa atgcatgcat tgattcatga tctgttagat tttgcaaaaa
     4441 tttccaaaga gcaatttgct tttaaagaaa t
//
LOCUS       DASTIB010000246         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_645476, whole genome shotgun sequence.
ACCESSION   DASTIB010000246 DASTIB010000000
VERSION     DASTIB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645476"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFP93_04255"
     CDS             <1..225
                     /locus_tag="VFP93_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843848.1"
                     /translation="FRLGLGVKQMLTPNFGAKIFYYWENTNRLTGSFAPAPHIGALIP
                     TIDTDFYNAQAKNNQLIGFGLFYQLCPSIF"
     gene            complement(255..>2116)
                     /locus_tag="VFP93_04260"
     CDS             complement(255..>2116)
                     /locus_tag="VFP93_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843849.1"
                     /translation="LKNTRVTMADIVLVALGLQFRAPKTEEKFQELRKKKYKELEELP
                     GDQFFAKSPSIELRNESNQVELIQESELIQWLNSRDRKEQLPFLQETFEQFEQTIKNQ
                     FVEKLQYLGTLTQQDEQAKTAYGVYKPFLTQHNFAKPILDCLAGEKYIYDNEDQQKAL
                     LIACLNASVTWEKLKKAILDVQKEIDGALQRIAAQKAALDRLSNVQVNQHNSDLFAIR
                     QSIKLDLLGDEQIEESEKQKALLDKLARHIFQQRYPEAILDAEDAILEEVQNKEAAIE
                     KVKDKIAVEKVRGTEEEVQRLQETLKKLEEENAMLKLELEALQKIQQEFVHNKEELAR
                     NKDELAQNQQELEIHKKLLANAQDELLCFKVIFEEQQQELMISTKDLANQKEQLHQVQ
                     MNLELQQKELEQSTFALSQQTALLSQTTVELEEQRKQLHNSRSVLEKQSNILQENKAA
                     FQHQAMELEKSKAILLEQNREIAKNKALLEQQQADLGKNKTAMQKQFVDLELKAAEVE
                     ENKRLLAQTQQALKSKQVELDKLKNTTYTPAMNMVRKETPAEVYLRTKSVDEMFGCAA
                     HMGYKTLEPLTYLAWMNVNRKDYSSTKIAVASQVWNYNATAGECFKNKYHPQS"
BASE COUNT          531 a          388 c          330 g          867 t
ORIGIN      
        1 ttccgattag gtttaggagt aaaacaaatg ctaaccccaa attttggggc taaaattttc
       61 tattattggg aaaatacaaa tcgattaact ggttcctttg ctcctgcacc tcatattgga
      121 gcattgattc ctaccataga cacggatttt tataatgctc aagcaaaaaa taatcaatta
      181 attggattcg gattatttta tcaattgtgc ccttcaattt tttaaaagta catctcttca
      241 gtcatgtact ttttttagct ttgtgggtga tatttatttt taaagcactc tccagcggtt
      301 gcattataat tccatacttg cgaagctact gcgattttag tagaagaata gtccttccta
      361 tttacattca tccatgcaag atatgttaga ggttctaaag ttttataacc catatgtgca
      421 gcacacccaa acatctcgtc cacactttta gttcgtaaat acacctctgc tggtgtttct
      481 tttcgaacca tattcatagc aggcgtgtac gtagtattct ttaatttatc taattctact
      541 tgtttggatt tcagcgcttg ttgggtttgt gccaataatc ttttattttc ttcaacttca
      601 gcggctttta attcaagatc aacaaactgt ttttgcatag cagttttatt tttccctaaa
      661 tccgcttgtt gttgttccaa taaagcttta ttttttgcta tttcacggtt ttgttccaat
      721 aaaattgctt tacttttttc taattccatc gcttggtgtt gaaaagcagc cttgttttct
      781 tgcaagatgt tactttgttt ttctaaaact gatctactat tatgtaattg cttcctttgt
      841 tcctctagtt caactgttgt ttgtgaaagc aaagctgttt gttgcgataa tgcaaacgta
      901 ctttgttcta attccttttg ttgcaattct aaattcattt gaacttgatg taattgttct
      961 ttttgatttg caagatcttt tgtactaatc attaattcct gttgttgttc ttcaaatata
     1021 actttgaagc agagtagctc atcctgtgca ttcgccaata attttttatg aatttctaat
     1081 tcttgttgat tttgggcaag ttcatcttta ttccttgcta gttcttcttt gttgtggaca
     1141 aactcttgtt gaattttttg caaagcttcc aactcaagtt ttaacattgc attttcttct
     1201 tcaagttttt tcaaagtttc ttgtaagcgt tgtacttctt cttctgttcc tcgtactttt
     1261 tctactgcaa ttttatcttt aaccttttca atagcagctt ctttattttg cacttcttct
     1321 aaaatagcat cttctgcatc taggatagct tctggatagc gttgttgaaa aatatggcgt
     1381 gcaagcttat ctaatagtgc tttttgtttt tcactttcct ctatttgctc atcacctaac
     1441 aaatccaatt ttatggattg tcgaattgca aataaatcgg aattatgttg atttacttgt
     1501 acattcgata atcgatcaag agctgctttc tgagctgcta ttctttgtaa cgctccatct
     1561 atttcttttt gaacgtccaa aattgctttt ttaagctttt cccaagtaac actggcgttt
     1621 aagcaagcaa taagaagcgc tttttgctga tcctcgttat cataaatata tttttctcct
     1681 gccaagcaat caagaatagg ttttgcgaaa ttatgttggg ttaaaaatgg tttgtatacg
     1741 ccataggcgg ttttcgcttg ctcatcttgt tgcgttaatg tgcctaaata ttgtaatttt
     1801 tcaacgaatt ggtttttaat ggtttgttca aattgctcga acgtttcttg cagaaaaggt
     1861 aattgttctt ttcgatctcg cgaatttaac cattgtatca attcgctttc ttgtatcaat
     1921 tcaacttggt tggattcatt cctcaattct atactaggtg attttgcaaa gaattgatcg
     1981 ccaggaagct cttcaagttc tttatatttc tttttacgca attcttgaaa tttttcctcc
     2041 gttttgggcg cgcgaaattg taaacctaat gctaccaaga caatgtcagc catagtcacg
     2101 cgggtatttt ttaact
//
LOCUS       DASTIB010000247         3962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1435984, whole genome shotgun sequence.
ACCESSION   DASTIB010000247 DASTIB010000000
VERSION     DASTIB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435984"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VFP93_04265"
     CDS             <1..1148
                     /locus_tag="VFP93_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9843850.1"
                     /translation="LRIIAGLIKPSKGQVLYKGKPVSRPVQGIAMVFQNFALLPWLTV
                     LQNVELGLEALGIPQAERRKRALKAIDTIGLDGFESAYPNELSGGMCQRVGFARALVV
                     EPELLLMDEPFSALDVLTAENLKGDLLDLWLNKSTRTKGILFVTHSIEEAALMADRIM
                     IFASDPGHIRAEIKVRTPLPRNAKDPHFRKLVDDIYTLMTTSQRLEEIRPEDAGYDYE
                     KMALSYRLPDVSISELTGLVDELAEYSTSVDLPDLADSLHLEADELFPLTEALEILRL
                     ATVSKGDIELTETGKEFASADILTRKKIFSHQLSQYVPLAQYIRKTLDESPNHVESEN
                     FFLGELQTSLSDTEAERVLKVIIDWGRYAEFFAYDYDTGNLSLENPK"
     gene            1157..2896
                     /locus_tag="VFP93_04270"
     CDS             1157..2896
                     /locus_tag="VFP93_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9843851.1"
                     /translation="MCHKKLLVLFLGIIIPAIAHAEKFVVFFQGVNQAQEQNIRNGLN
                     LLEQARTKELTRDEIRAFYESGKEEIRLALQPFGFYDVQVDSQFIDTKSPLQITYRIT
                     LNRVTRVDKLHIGLTGDGSENSQLFRSINVFPLKKGDPFNHQTYESGKNVLIQEAINQ
                     GFIKAYFNKHQVVVDLSEFTADIDLSLDTGPQYHMGEIHFNLGYYDHTFLQRFKTFSN
                     DDPYRLEEVVELESRLLQSNLFSSIYTTPSFDESQHSVPLNIVLEEAPANRYLVGAGY
                     DTNTRLRGRLGWERKKVNPFGHQVQTHFKVSEKDTLLNGHYIIPGKKPWVDQYRFSLG
                     YANEEYKDKPSELYTIEVSEIRLLSAWTRTLALRYLREDFKDSDFIKESSTFLLPSIT
                     FSRSKLDHVTTPRNGYRLQLSVRGGLDALLSDHDFIQFNAQYKWLKAISEEGTLHART
                     ELGATIPNQIAELPLSIRFYAGGNDSIRGFRYRSLPNEIDKEGNLQPVGGAYLLLGSL
                     EYDHTLWGPLKWAIFLDGGSALRKIEDDLQLGAGVGLRWQTPIGPIKLDVAKPLTNAA
                     EVLRLHFSFGPEL"
     gene            3109..>3962
                     /locus_tag="VFP93_04275"
     CDS             3109..>3962
                     /locus_tag="VFP93_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843852.1"
                     /translation="MRHIVKTVFLILGKIGFWVAFLIFGLWFTLNTTLGADLIFKTLS
                     WVTPWSIQYKALEGSLLSSLHFFDLSMRSSTMNLSAQRLDIDWDFHSLWGGTLHIRNF
                     QAAHVGIEPHISPSGPQKNFTLSEIRSEIHEAIPFPLRIDNFLATDVAIKTKKTILLK
                     HFSFNSANTTNSFVLNNLTVEGSFGQIKLEADQAIHAQWNIAIPDLSLWWNDLKGDFR
                     SMGKLDIRDIEQPLDSTIMELEVLSQSLVWNDLPFKDLKIHHKGSLKKTALSLEGIFK
                     DHLIKTHI"
BASE COUNT         1223 a          766 c          779 g         1194 t
ORIGIN      
        1 tgcttcgaat tattgcaggc cttatcaagc cttcaaaagg tcaagtactt tataaaggaa
       61 agcctgtatc aagacccgta caaggtattg ctatggtttt tcaaaatttt gctcttttac
      121 cttggcttac cgtactacaa aatgtggaac tcggtttaga agctttaggg atcccacaag
      181 cagaaagacg aaaacgtgcc cttaaagcta ttgataccat aggattagat ggatttgaat
      241 cagcttaccc caatgagctt tccggtggaa tgtgccaaag agtcggtttt gctagagctt
      301 tggtagtcga acctgaattg ctattaatgg atgaaccatt ttctgcctta gacgtgttaa
      361 cagcggaaaa tttaaaaggc gatcttctag atctatggct caataaatcg acccgcacga
      421 aaggcatttt atttgtaacc catagtattg aagaagccgc tttgatggcc gatagaatta
      481 tgatctttgc cagtgatccc ggacacattc gagcggaaat taaagtgcgt acaccgctac
      541 cacgcaatgc caaagatccg cattttagaa agttggtcga tgatatttat acgttaatga
      601 ccacaagtca aagattggaa gaaattcgtc ccgaagatgc aggttacgat tatgaaaaaa
      661 tggccttaag ttatcgcttg cccgatgtta gcatttcaga actcacaggc cttgtggatg
      721 aattggcaga gtattcaacc tcggttgact tacctgattt agcggatagc ttacatttag
      781 aagcagacga attattccct cttaccgaag cgctggaaat tttacgactt gcgaccgtgt
      841 cgaaaggtga tatagaactt acggaaacag gcaaggaatt tgcctcagct gacattttaa
      901 cacgtaaaaa aatattttct caccaactgt cgcaatatgt tccgcttgcg caatatatcc
      961 gtaaaacttt agacgaaagt cctaaccatg ttgaatcgga aaatttcttt ttgggagagc
     1021 tgcaaacttc gctgagcgac accgaagctg aacgggtatt aaaagttatt atcgattggg
     1081 gtcgttatgc ggaatttttt gcctatgatt atgataccgg gaatctcagc ctagaaaatc
     1141 ctaaataagt aacattatgt gccataaaaa attgctggtt ctctttctag gaattattat
     1201 tcctgctatt gcgcatgctg aaaaatttgt cgtttttttt caaggtgtca atcaagctca
     1261 agaacaaaac attcgcaatg gcttaaattt attagagcaa gctagaacaa aagaattaac
     1321 acgggacgaa attcgtgctt tttatgaaag tggcaaagag gaaattcgct tagctttgca
     1381 accattcggt ttttatgatg tacaagtgga ttcccaattc atcgatacga aatctccatt
     1441 gcaaattacc tatcgtataa ccttaaatcg tgtaacccgg gtggataaat tacatattgg
     1501 acttaccgga gatggtagcg aaaattctca attatttcgc agtatcaacg tatttccgtt
     1561 aaaaaaaggc gatcccttta accaccaaac atatgaaagt ggaaaaaacg ttttaatcca
     1621 agaagccatc aaccaaggtt ttattaaagc atattttaat aagcatcagg tggttgttga
     1681 cttaagtgaa tttactgcag atattgattt gtctttagac accggcccgc aatatcacat
     1741 gggcgaaatt cattttaact taggatatta cgatcatact tttttgcaac gtttcaaaac
     1801 tttttccaac gatgatccgt atcgcttaga agaagtagtt gaattagaat cgcgcttatt
     1861 acaaagcaat ctatttagca gtatttatac cacgccctcc tttgacgaaa gccaacacag
     1921 tgtaccttta aatattgttt tagaagaagc gcctgccaat cgttatcttg taggtgccgg
     1981 ctacgatacc aatacgcgtc ttaggggtag attgggttgg gaacgtaaaa aagttaaccc
     2041 ctttggacat caggtacaaa cccactttaa agtttccgaa aaagacacgc tgctcaatgg
     2101 ccattatatc attcctggca aaaaaccttg ggtggatcaa tatcgttttt cgttaggcta
     2161 tgccaatgaa gaatataaag acaaaccttc cgaattgtat accattgagg taagtgaaat
     2221 tcgattactg tccgcttgga cgcgcacttt agctttacgt tatttacgcg aagactttaa
     2281 agatagtgat tttataaaag aaagttctac ctttttacta cccagcatta cctttagtcg
     2341 atcgaaactg gatcatgtaa ccaccccaag gaatggttat cgtttacaac tatcggtacg
     2401 tggtgggtta gatgcacttc taagtgatca tgattttatt caatttaacg cgcaatacaa
     2461 atggttaaaa gcaatatctg aagaaggcac actccatgcg cgcacggaat taggcgcaac
     2521 catacccaac caaattgccg aactgccgtt aagtattcga ttttatgcgg gtggtaatga
     2581 cagtataaga ggatttcgtt atcgatccct ccctaatgaa attgataagg aaggaaattt
     2641 acaaccggtg ggaggcgctt atttattatt aggcagcctt gaatatgatc atacgctttg
     2701 gggtccctta aaatgggcga ttttcctaga tggtggtagt gcgttaagaa aaatagaaga
     2761 tgatttacaa ctgggtgcag gtgttggctt acgttggcaa actccgattg gacctatcaa
     2821 acttgatgta gcgaaacctt taaccaatgc cgccgaagtg ctgcgattgc attttagctt
     2881 cggtcctgag ttataaatag gagctcgcga taagcactct ttctgaaaag aacaccagga
     2941 aaagcctgag cacgaaatac aggcatgcta cacatattat gttcttacac taaataatgc
     3001 gttttctaaa aacctatcta tgccgaatat tttaagttac ttgaaaaact ttctatgcat
     3061 tctacgccat atcgcttaca ctgatttatt tattgcagac taggacctat gcgccacata
     3121 gtcaaaaccg tgttcttgat cctaggaaaa ataggatttt gggtagcctt cttgatcttt
     3181 ggcttgtggt ttaccttaaa taccacttta ggcgcagatt tgatttttaa aaccctatct
     3241 tgggtcacgc cttggtctat tcaatataaa gcattagaag gttccttatt aagttcactg
     3301 catttttttg atttatcgat gcgttcctca acgatgaatt taagtgccca acgtttagat
     3361 atcgattggg attttcattc tttatggggc ggtaccctcc acattagaaa ttttcaagca
     3421 gcgcatgttg ggattgaacc acacatatcc ccgtctggac ctcaaaaaaa ctttacgttg
     3481 agcgaaattc gatccgaaat acatgaagct attccctttc ctttacgtat tgacaatttt
     3541 cttgcgaccg acgttgcgat aaaaacaaaa aaaaccatcc ttttgaaaca tttttctttt
     3601 aattccgcga atacgactaa tagctttgtg ttaaataatt taaccgttga aggttcgttt
     3661 gggcaaataa aacttgaagc ggatcaagca attcacgccc aatggaatat agctataccc
     3721 gatttgtcgt tgtggtggaa cgaccttaag ggcgattttc gtagtatggg aaaattagat
     3781 attcgcgaca ttgaacagcc gctcgattca accataatgg aattggaagt tttaagccaa
     3841 tcgttagtct ggaatgattt accgttcaaa gatttaaaaa tacaccacaa aggttcttta
     3901 aaaaaaaccg cactttcttt agaaggtatt tttaaagatc atttaataaa aacgcatatt
     3961 ca
//
LOCUS       DASTIB010000248         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_616027, whole genome shotgun sequence.
ACCESSION   DASTIB010000248 DASTIB010000000
VERSION     DASTIB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616027"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /gene="thyA"
                     /locus_tag="VFP93_04280"
     CDS             <1..281
                     /gene="thyA"
                     /locus_tag="VFP93_04280"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008039363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET9843853.1"
                     /translation="GVPFNIASYALLTHMIAQVTGLQAHEFVHTFGDVHLYANHIEQA
                     KEQLKREPFTLPRLVINPNVSQIDDFVFEDFAIENYEAHPHIKAAVAV"
     gene            281..787
                     /locus_tag="VFP93_04285"
     CDS             281..787
                     /locus_tag="VFP93_04285"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET9843854.1"
                     /translation="MSIQLIVAMSENRVIGKDNKLPWHLPNDLKHFKNLTLGKAVLMG
                     RKTFESIGKLLPDRTNIVLTTDPAYVISSGIVVNSIEEALAIGETFPELMVIGGETLF
                     RQCISIADTMHLTLVHTTIDGDTFFPAFDQNIWQEVERIDMPKDEKHVFAYSFLTLKN
                     TEFLTTSE"
     gene            803..1942
                     /locus_tag="VFP93_04290"
     CDS             803..1942
                     /locus_tag="VFP93_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843855.1"
                     /translation="MLFMQAVELIKNQINHYMIKKLDLAKTLDPEAILSGLLARRDEQ
                     TQGIMNSQAKPSYLERLLRFNSELTWEKMMQCDFATFRSILNNNLTQELVVTNKLMVL
                     ARLKYQLQTKLEESKLQFQTRQSQSTLENVKTAEQASLHECIDEQLIAFTLKNIEEAE
                     ALLEVARSQSSQKIMVSQISVTAMPGNTELLLQACKSFIREFYHFCDDIDRKYNRLPG
                     SMYALRDYLPDQAKLDHPIVLTQNEASKYILHNLWEEAKQVQVKEKPKNKYLKLGGLL
                     ELGQDLWKQIRHDPQLACNKRELMYRLFENLSNINTDQTREEIQQQVLNVIDTHYGSA
                     KSYTPLRSLVSHQAIEGSFHKIHETLLEKAPDAVKIQHMRSCNFT"
     gene            1957..>2263
                     /locus_tag="VFP93_04295"
     CDS             1957..>2263
                     /locus_tag="VFP93_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HET9843856.1"
                     /translation="MKKYKNPEMLSSVVYFLSRCLHTSLRIQKFKHPTVDERKPAIYC
                     FWHGHLYLPIITVKKILQHPAAGFVSYSKDGEILARWLEKMGYSVVRGSSSKKGFAGM
                     "
BASE COUNT          771 a          416 c          426 g          650 t
ORIGIN      
        1 taggcgttcc gtttaatata gcttcttacg ctttgttgac tcatatgatt gcacaagtaa
       61 ccggtttaca agcacacgag tttgttcata cgtttggcga tgtacatttg tatgcaaatc
      121 acatcgagca agcaaaagaa caattaaaaa gagaaccgtt taccttgcct agacttgtaa
      181 taaaccccaa cgtttctcaa attgatgatt ttgtgtttga agattttgcc atagaaaatt
      241 acgaagccca tccacatatt aaagcggctg tggctgtgta atgagcatac aattaattgt
      301 tgccatgagc gaaaatcgtg ttattggaaa agataataaa ttgccgtggc atttgcctaa
      361 tgatttaaaa cattttaaaa atttgacgct aggcaaagct gtacttatgg gtcgaaaaac
      421 attcgaatcc attggtaagc ttttacctga cagaaccaat atcgttttaa caacggatcc
      481 agcctatgta atatcttccg gtatagtagt gaattccatc gaggaagcgt tggcgatagg
      541 agaaactttt ccggaattaa tggttatagg aggggaaaca ttgtttcgcc agtgtatcag
      601 catagcggat accatgcatt taactctcgt acataccacg atcgacggag acactttttt
      661 tccagctttt gatcaaaata tttggcaaga ggttgaacgc atcgatatgc caaaagatga
      721 aaaacatgtt tttgcgtatt cgtttttaac tttaaagaac accgaatttt taacaacctc
      781 agagtaaaat tcagggggac gtatgctttt catgcaagcg gttgaactga taaaaaatca
      841 aattaatcac tatatgataa agaaattgga tctcgcaaaa acattagatc ccgaagcaat
      901 attatctgga ctccttgctc gacgcgatga acaaacgcaa ggaataatga attcccaagc
      961 aaaaccttcc tatttggaaa gacttttgcg atttaatagt gagcttacct gggaaaaaat
     1021 gatgcaatgc gattttgcaa ctttccgatc cattcttaat aataatttaa cccaggaact
     1081 tgtagttacc aacaaattaa tggttttggc cagactaaaa tatcaattac aaacaaaatt
     1141 agaagaaagc aaactacaat ttcaaacccg ccagtcacaa tcaacgcttg agaatgtaaa
     1201 aactgcggag caagcatcgc tccatgaatg tattgatgaa cagctcatag cttttacgtt
     1261 aaaaaatata gaagaagcgg aagctttatt agaagtagcc cgcagtcaat ctagtcaaaa
     1321 aattatggta agtcaaattt cggttaccgc catgccgggt aacacagaac ttttattgca
     1381 agcgtgtaaa tcttttattc gagaatttta tcatttttgt gatgatatag atagaaaata
     1441 taatcgtctc cccggatcta tgtatgcatt acgcgattat ctgcccgatc aggcaaaatt
     1501 ggatcatcct attgttttga cgcaaaacga agcaagcaaa tatatattgc ataatttatg
     1561 ggaagaagca aagcaagtgc aagtaaaaga aaaaccaaaa aataaatatt taaaattggg
     1621 tggcttatta gaattgggac aagatttgtg gaaacaaatt aggcatgacc cacaacttgc
     1681 ttgcaataag cgcgaactca tgtatcgatt gtttgaaaat ttatccaata ttaataccga
     1741 tcaaacgaga gaagaaattc aacaacaggt attaaacgtt atcgacacac attacggttc
     1801 agccaaaagc tatactccat tacgtagcct agtttctcat caagctattg aaggaagctt
     1861 tcacaaaatt cacgaaacgc tcttggaaaa agcacccgat gctgtcaaaa tccaacatat
     1921 gcgttcatgt aattttactt aaagcactcg gctatcgtga aaaaatataa aaatcctgaa
     1981 atgttatcgt ctgttgtgta ttttttatcg cgctgtttac acacttcttt gcgcatacaa
     2041 aaatttaaac atcctacggt ggatgagcgc aagcctgcca tctattgctt ttggcatgga
     2101 catttatatt tgcctattat tacggtaaaa aaaattctgc aacatccggc agccgggttt
     2161 gtgagctatt ccaaagatgg agaaatttta gcacgttggc tcgagaaaat gggctattcg
     2221 gtcgtgcgcg gatccagctc aaaaaaagga ttcgccggta tga
//
LOCUS       DASTIB010000249         5312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1152415, whole genome shotgun sequence.
ACCESSION   DASTIB010000249 DASTIB010000000
VERSION     DASTIB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152415"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="der"
                     /locus_tag="VFP93_04300"
     CDS             complement(<1..851)
                     /gene="der"
                     /locus_tag="VFP93_04300"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9843857.1"
                     /translation="MKSLTLAIVGRANVGKSTLFNCLTRSRNALVADKEGLTRDRLYG
                     NCVLEGIPYQVIDTCGVDGEQNEELSHFMDQQVQNAVVEADCALMVVDGQKGLLAEDI
                     ALAKRLRIANKKTYIVVNKAEGKEPTYVCAEFYALGFDKVFAISAAHNQGILTMMEEV
                     AQDNQLSAPEETTSDSDLGLKIAILGRPNVGKSTLINRLLQEDRVVVFDMPGTTRDSI
                     YIPFKRHQKNYTLIDTAGVRRKSRVSDLVEKFSAIKTLQAMQDADCVILVLNAHEDLY
                     DQDLNLLR"
     gene            complement(848..2041)
                     /gene="bamB"
                     /locus_tag="VFP93_04305"
     CDS             complement(848..2041)
                     /gene="bamB"
                     /locus_tag="VFP93_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HET9843858.1"
                     /translation="MNNLVVKGFGCALVFLMMTGITGCNHSPIRNPFKSQKIVLGPLP
                     PLEEQTTAEIVWRQRTTKGAGDFAKIRPTLENNTIYQADHSGILVAINKANGRKFWRK
                     ITKHPIASGPVLIQNNLYVGTKNGKVVAFNPQSGEELWQARVPSEVLAPPQGNQEVIL
                     VNTIDGQLTALAADSGKILWSYQRVIPSLTLRGGSSPALAHDQALAGFANGKLVALEV
                     KTGSMAWERTITVPRGRSELHRMVDMNSNPIIVGDTVFVASYHGNVAGIDINSGQVKW
                     QHQLSSYRDMEADDKALYVTDNEHHVWALDLETGGTLWKQAALSQRKITGPALTDDYL
                     VVGDHGGYVHWLRKSDGQLLGRYGIGKQILERPVAQGNLVLVTAVYGEMTALRLPSMA
                     ENRSS"
     gene            complement(2038..2700)
                     /locus_tag="VFP93_04310"
     CDS             complement(2038..2700)
                     /locus_tag="VFP93_04310"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9843859.1"
                     /translation="METYVTEEQQLDAIRKWFKEHGKTLLTAISLAVLLALGIRYWLH
                     HKEVVRLKASDQYMAMVLNQNQEDVQSMQTKAQTLMKDFSHTPYASLAALQLAKHAVE
                     TQEFAKALQHLQWVIDNSSVVEFQLVARARAARILMHLGQYEKALQLCTVTNSDGYTS
                     LLEELKGDIYVAQNKPELAKKAYTAALKSTKEKDLEHPLLRMKLQELGGTEFQNGEET
                     TS"
     gene            complement(2738..4006)
                     /gene="hisS"
                     /locus_tag="VFP93_04315"
     CDS             complement(2738..4006)
                     /gene="hisS"
                     /locus_tag="VFP93_04315"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET9843860.1"
                     /translation="MALLQAIRGMRDLLPEETALWHAVEYSLWELMVSYGYQEIRMPL
                     LEKTQLFTRGIGEATDIVEKEMYTFDDRNGESVTLRPESTAQCIRAGLEHGLFYNQTP
                     RLWYYGPMFRYERPQKGRYRQFYQFGVETYGIATPDIDAEQILMMHRFWKQWDILSQI
                     TLEINSLGTDVCRKAYRVALVEYFTRYSADLDEDSQRRLGSNPLRILDSKNESMRDLI
                     NNAPKLMDFIHVEARDHFEQLRHYLDEASIAYKVNPNLVRGLDYYGLTVYEWTTTSLG
                     AQGTVCGGGRYDNLVGLLGGPETKAVGFAVGMERLISLIQTQTKHNTAHLDAYFIPMG
                     DKAQKSSIYIAEQIRNIAPHKTLLTHCGGGSVKSQFKKADKSGATIALIVGDLELENK
                     TVTVKYLREEKAQRTVALTHLPEIFKEMTD"
     gene            complement(4151..4564)
                     /locus_tag="VFP93_04320"
     CDS             complement(4151..4564)
                     /locus_tag="VFP93_04320"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HET9843861.1"
                     /translation="MPAAKGDKQVAGTFLGGVGGALIGSTIGAGSGQVIATAAGAVLG
                     ALVGNQMGQQLDETDTIVAHRSFYRATEGEIGHPHYWCDETTGTSGYIRPIRDGMYQG
                     LYCREFQTTIYIDGRTERLYGTACRLRDGRWVPVT"
     gene            complement(4742..>5312)
                     /gene="pilW"
                     /locus_tag="VFP93_04325"
     CDS             complement(4742..>5312)
                     /gene="pilW"
                     /locus_tag="VFP93_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HET9843862.1"
                     /translation="KLGPDLPEVHYALGYYKESMGEVDQAKDHYLEALRKKPKHGIAH
                     NNYGAFLCRQGHYQEAEKEFLTAVEDPEYTTPAEALENAGLCVLQIPDVKRAQEHLER
                     SVRMDPKRFSAILELSMINYQNKKYSTSLEYFSLFAQQADHSPRSLWLGIKLAEHYQD
                     QKRLLNYKKILKEKFPQSAEYKFLEKNTS"
BASE COUNT         1423 a         1186 c         1026 g         1677 t
ORIGIN      
        1 cgcagtaaat taagatcttg atcgtataaa tcttcatgtg catttaacac caaaataacg
       61 caatcggcat cctgcattgc ttgtaaggtt ttgatagctg aaaacttttc aactaaatcc
      121 gaaactctac ttttacgcct aacgccagcg gtatcgatca aggtgtagtt tttttggtgt
      181 cttttaaagg gaatgtaaat gctgtcacgc gtagtgccag gcatatcaaa cacgactacc
      241 ctatcttctt gtagcagacg gttaatgagg gtagatttcc ctacgttagg tcttcctaat
      301 atcgcaattt ttaaacctag atcactgtca gatgtagtct cctccggtgc acttagttga
      361 ttgtcttgcg ccacttcttc catcatggta agaatacctt gattgtgggc tgcggaaatg
      421 gcaaatactt tatcaaagcc taatgcataa aattccgcac agacataagt aggttctttc
      481 ccctcggctt tattgacaac gatgtaggtt tttttgtttg caatccgcaa tctttttgcc
      541 aatgctatgt cttctgccaa taatcctttt tgaccatcta ctaccatgag tgcacaatcg
      601 gcctctacta cagcgttttg tacttgctga tccatgaaat gggagagttc ttcattttgc
      661 tctccatcca cgccacacgt atcaattact tggtagggaa tgccttctaa gacacaattg
      721 ccataaagcc tatcgcgcgt taagccctct ttatcggcca caagcgcatt tctgctacgc
      781 gttagacagt taaataatgt tgatttgcct acattagcac ggcctacaat agctaacgtt
      841 aatgatttca cgaggatcga ttctccgcca tagaaggtaa cctaagggcg gtcatttcac
      901 cataaaccgc tgtaactaat actaaatttc cttgtgcaac tggtcgctcc aaaatttgtt
      961 ttccgatgcc ataacgacct aataactgac catcactttt acgcaaccag tgtacatagc
     1021 ccccatgatc gcccacaact aagtaatcat cggttaaagc agggcccgta attttacgtt
     1081 gacttaaagc tgcttgtttc caaagtgtac caccggtttc taaatctaag gcccatacat
     1141 gatgttcatt gtctgtgaca taaagcgctt tatcgtctgc ttccatgtcg cgataagacg
     1201 aaagctgatg ttgccatttc acttggccgc tattgatatc aatgcccgcc acgttgccgt
     1261 ggtaagacgc aacaaatacg gtatctccta cgataatcgg gttgctattc atatccacca
     1321 tacgatgtag ctcagaacgc cctctgggta ctgttatcgt gcgttcccaa gccatggagc
     1381 ccgtttttac ttctaaagca actaatttac cgtttgcaaa acccgctaac gcttgatcat
     1441 gggcgagagc aggcgaacta ccacctcgca atgttaagga aggaataaca cgttggtaac
     1501 tccacaaaat tttaccggaa tcggccgcaa gagcggttaa ttgcccatcg atggtgttta
     1561 ctaaaatgac ttcttgatta ccctgcggag gggctaaaac ttcgctgggt acgcgcgctt
     1621 gccataactc ctcgccggat tgcggattaa aagccacgac tttgccattt tttgtaccga
     1681 cgtataaatt attctgaatt aaaacagggc cacttgcgat cggatgtttt gttattttgc
     1741 gccaaaattt tctaccgttg gctttgttaa tcgctaccaa tataccggaa tgatcggctt
     1801 ggtaaatggt attattttct aatgtgggac gaatttttgc aaaatcgccc gcacctttgg
     1861 tagtacgttg acgccaaaca atttctgccg tagtttgttc ttctaaaggc ggtagaggac
     1921 ctaatactat tttttgtgat ttaaaaggat tgcggatagg actatggttg caacccgtaa
     1981 tccctgtcat cattaaaaaa acaagtgcac atccaaagcc cttgactact aaattattca
     2041 tgatgtggtc tcttccccat tttggaattc tgttccaccc aattcttgca atttcatgcg
     2101 taacaagggg tgttctaaat ctttttcttt tgtggatttc aaggctgcgg tatacgcttt
     2161 ttttgctaat tcgggtttat tttgtgcaac ataaatatcg cctttgagtt cttcgagcaa
     2221 ggaagtataa ccatcactgt tggttaccgt acacaattgc aacgcttttt catattgtcc
     2281 aagatgcatt aaaattcttg ctgcacgagc acgtgctacc aactgaaatt ctaccacaga
     2341 agaattatcg ataacccatt gcaaatgttg aagtgctttg gcgaattctt gcgtttccac
     2401 agcatgctta gctaattgta atgcagccaa agacgcatat ggtgtatgtg aaaaatcttt
     2461 catcaatgtt tgtgctttgg tttgcatcga ttgcacatct tcctgatttt gatttaaaac
     2521 cattgccata tattgatcgg atgcttttaa tcgaaccacc tctttatggt gtaaccaata
     2581 gcgtattcct aacgcaagca aaaccgctaa acttatggcc gttagtagtg ttttgccatg
     2641 ttctttaaac cattttcgga tggcatctaa ttgttgttct tcggttacgt atgtttccac
     2701 ttaagacctc ctcaattttg cgacacttag cgcgagatta atctgtcatt tccttgaata
     2761 tttcgggcag atgtgtaaga gctaccgtac gctgtgcctt ttcttcgcgc aagtatttca
     2821 ccgttacggt tttattttcc aactccaaat cgcccactat gagtgcgatt gtagcgcccg
     2881 atttgtctgc tttcttaaat tgcgatttta cgctaccccc gccacaatgc gttaatagcg
     2941 ttttatgagg ggcaatattg cgtatttgct ctgcaatata aattgaactc ttttgtgctt
     3001 tatcccccat gggaataaaa tacgcatcta ggtgtgcggt gttatgcttt gtttgtgttt
     3061 gtattaagct aataagacgt tccattccca ctgcaaaacc tacggctttt gtttcgggcc
     3121 cacctaataa acccactaaa ttatcataac gtccgccacc acaaacggtt ccttgcgccc
     3181 ctaaagaggt agttgtccat tcatatacgg tcagtccata ataatcaaga ccacgcacga
     3241 gatttggatt tactttataa gcaatactgg cttcatctaa atagtgacgc aattgttcaa
     3301 aatgatcgcg agcttccaca tggataaaat ccattaattt gggagcattg tttatcagat
     3361 cacgcatgga ttcattttta gaatctaata tgcgaagcgg gttactgcct aaacgccgtt
     3421 ggctatcttc atctaaatct gctgaataac gggtaaaata ctctacaagt gcaacccgat
     3481 atgcttttcg gcaaacatcc gttcctaggg aattaatttc aagtgttatt tgcgaaagaa
     3541 tatcccattg tttccaaaat cgatgcatca taagaatttg ctcagcatcg atatcgggag
     3601 tagcaatacc ataggtttct accccaaatt ggtaaaattg gcgataccga cctttttgcg
     3661 gacgttcata gcgaaacata ggaccataat accaaagacg gggggtttgg ttataaaaca
     3721 aaccatgttc caaacctgct cgtatacatt gggcagtact ttcggggcgc aatgtaacac
     3781 tttctccatt gcgatcgtca aaagtataca tttctttttc aactatatcg gtagcttcac
     3841 caatgccccg tgtaaataat tgggtttttt ccagtagggg cattctgatt tcttggtagc
     3901 cataacttac cattaattcc cataaagaat actcaactgc atgccaaagc gcagtttcct
     3961 ctggaagcaa gtcgcgcata ccgcgaatgg cttggagtaa agccatgatt gatgaacctt
     4021 aaaataccat taattgttgc atactttatc aggagcgcgg cttgaacgcg actctctgac
     4081 aaacatttgg ttgattaact tgtgatattt taaggtaaaa aaatagagct gcaattgtta
     4141 gttaattgaa ttaagtaact ggaacccaac gcccatccct taaacgacat gctgtaccat
     4201 aaagacgctc ggtccgacca tcaatataaa tcgtggtttg aaactcgcga caatataatc
     4261 cctgatacat gccatcgcga ataggtctta tgtatcctga agtgccagtg gtttcgtcac
     4321 accagtaatg gggatgtcct atttcacctt ctgttgccct ataaaaagaa cggtgggcaa
     4381 ctatagtatc ggtttcgtct aattgctgtc ccatttgatt gccgactaaa gcacccagta
     4441 ctgcacctgc agcggttgca atgacttgtc ctgaacctgc gcctatggtt gaaccaataa
     4501 gtgcccctcc tacaccccct agaaaagttc cggcaacttg tttgtcacct tttgcagcag
     4561 gcatttgatg acttgtgcac cctaccaaac tcacgctgga aacaactaaa caagttatca
     4621 gtatcgctcg tctcatcaac cctatccttt tttttggggc taatttgaat atagtcgatg
     4681 cttttatacc gtcgaaacct atttaagcaa ttttttcaag ttagagttat gctgcagtga
     4741 attaagaagt atttttctca aggaatttat actctgccga ttgtgggaat ttttctttga
     4801 gtattttttt gtagtttagt aagcgttttt gatcttgata atgctctgcc aatttaatac
     4861 cgagccatag cgatcttggg gaatgatcgg cttgttgcgc aaacaaacta aaatactcta
     4921 aagaagttga gtatttctta ttttggtaat taatcatact tagctcaaga atagcagaaa
     4981 atcttttggg atccatacgt acggatcgtt ccaggtgttc ttgcgcacgt tttacatcag
     5041 gaatttgcag aacacaaagt cctgcgttct ctaaagcttc tgccggcgtc gtatattcgg
     5101 gatcttctac tgcagttaaa aactcttttt ccgcttcctg atagtggcct tgtcggcaca
     5161 aaaaggcgcc ataattattg tgggctatgc catgtttagg tttttttcgg agtgcttcga
     5221 gatagtgatc tttggcttga tctacttccc ccatagactc tttatagtat cctaatgcgt
     5281 aatggacctc aggtaaatca ggccctaatt tt
//
LOCUS       DASTIB010000250         5111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_535521, whole genome shotgun sequence.
ACCESSION   DASTIB010000250 DASTIB010000000
VERSION     DASTIB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535521"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /gene="nuoL"
                     /locus_tag="VFP93_04330"
     CDS             complement(<1..1758)
                     /gene="nuoL"
                     /locus_tag="VFP93_04330"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9843863.1"
                     /translation="MQQTIFYYCVIITLAPLIAASFAGLLGRKISNVQAHSVTILGVA
                     IAFVFSCLALYTVKFTNTVSPINLTAYIWGTTGNLSLQIGFLIDELSTLMMVIVTFVS
                     LMVHIYTVGYMQGDPGYKRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLMSYLLI
                     GFWYKKPSAIQANLKAFIVNRVGDFGFLLGIAGVFLLFNSLDYTTVFNQVETIVGANQ
                     FFIFSDEVMFPAIHVICILLFVGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVT
                     AGVFMVARLSPIFEHSDVALNFILIIGSITAFFMGLIGLVNQDIKRIIAYSTLSQLGY
                     MMVAVAVSSYAVGIFHLMTHAFFKALLFLGAGSVIIGVHHEQNIWKMGGLRRKMPITF
                     LCMLIGTLAIVGMPFFSGFFSKDLIIESVAFSNLRAAKIAYWAVLAGVFVTSLYSFRL
                     LFVVFFGKTRMDSDSFHHVHESPSVVWLPLVTLAIPSIVSGYLFIKPLLHGYFGNALQ
                     ALPEHTPLPLFLEEFHGALSMALHGFATLPFFFMLAGLGCAVVLYVIIPSLPMHLKKV
                     LKPFYLILEHKFGFDLFYNW"
     gene            complement(1760..2065)
                     /gene="nuoK"
                     /locus_tag="VFP93_04335"
     CDS             complement(1760..2065)
                     /gene="nuoK"
                     /locus_tag="VFP93_04335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9843864.1"
                     /translation="MISLSDYLIFSAILFGIGMAGIFINRRNLIVLLMCIELILLAIN
                     TNFIAFSHFLENLDGQIFVFFVMTVAAAESAIGLAILVVLFRKKQTINVEEIDLLKG"
     gene            complement(2062..2667)
                     /locus_tag="VFP93_04340"
     CDS             complement(2062..2667)
                     /locus_tag="VFP93_04340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9843865.1"
                     /translation="MFESLIFYFFGLVALTASLGVIFSQNPVRAVLFLVLTFFTSAAI
                     WLMLEAEFLAIALVLVYVGAVMVLFLFVVMMLDIEVGTLKLSFKRHIPLGLFILCLMI
                     ATLILAIGPHHFGLDYIIVPERASASYSNIEALGELLFTQYILPFEVAGVILLVAMIA
                     AIALAFRGHRSRKQNIQKQIFTTKQDRLHMVKMPSETGEAL"
     gene            complement(2677..3168)
                     /gene="nuoI"
                     /locus_tag="VFP93_04345"
     CDS             complement(2677..3168)
                     /gene="nuoI"
                     /locus_tag="VFP93_04345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET9843866.1"
                     /translation="MLKRLKQYIKSFMLWELLLGLKLTGKYLFKRKITIYYPEETTPV
                     SVRFRGIHALRRYPNGEERCIACKLCEAVCPALAITIESYEREDGTRRTSRYDIDLFK
                     CIYCGFCEESCPVDSIVETDQLNFAILERGQNILDKQKLLAIGDKLEPSIAKNREEDA
                     PYR"
     gene            complement(3192..4220)
                     /gene="nuoH"
                     /locus_tag="VFP93_04350"
     CDS             complement(3192..4220)
                     /gene="nuoH"
                     /locus_tag="VFP93_04350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET9843867.1"
                     /translation="MFDTLFAVGWILLKIILIIVPVMLSVAYLTFVERKVLGYIQCRV
                     GPNRVGYYGLLQPIADAIKLLFKEIILPTHANKFLYIVGPILTVAPALIAWSVIPFDY
                     GLVLANLNIGLLFLLAMTSMGVYGIIIAGWASNSKYAFLGAMRSAAQVVSYEIAMGFC
                     LVGVLMASGSMNLTNIVLAQQGSIIHWYWLPLLPLCVIYWISAVAETNRNPFDVAEGE
                     SEIVAGFHVEYSGMTFALFFLAEYANMILLAALASIMFFGGWLSPFEGIPLLEEFFSY
                     VPGIFWLLLKIAFLVFLYLWFRGTFPRFRYDQIMRLGWKIFIPVTLVWIVVLAVAIKL
                     NLRPWFAA"
     gene            complement(4224..>5111)
                     /locus_tag="VFP93_04355"
     CDS             complement(4224..>5111)
                     /locus_tag="VFP93_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9843868.1"
                     /translation="ANITQAKIGEIPTGANASGAWIAGFIPHRGPVGETLDLNAGKTA
                     LEMLHEPLASMVLMNFEPEFDAYHPALSLETLTKVQEVICITPFVTDRMRSYASILLP
                     MALSSETDGTFINMTSDWQSFKAVAKPYGNSKPAWKILRVLANLFKLPGFQYNKAKEI
                     TFEIRKNFEQAPVIENQILHKIPEKIEPPLKDRLIRLAPMAMYRTDSLVRRAQCLQET
                     IETQSNKKIGLHPETADHLQIQEGAKVKAQQQGRITHEAFEVQLNPNVPKGSILLYAG
                     FEETLLGAPYSVITLERVG"
BASE COUNT         1786 a         1046 c          905 g         1374 t
ORIGIN      
        1 ccagttataa aaaagatcga aaccaaattt atgttccaaa ataaggtaaa agggttttaa
       61 tacttttttc aaatgcatgg gtaaagatgg aataatcaca tacaagacca cagcacaacc
      121 taagcctgca agcataaaaa agaacggtaa tgtggcaaaa ccatgtaacg ccatagacaa
      181 agcaccatga aattcttcta aaaataaagg taacggtgtg tgttctggca gagcttgcaa
      241 tgcattgcca aaatatccat gaagaagtgg tttgataaat aaatagcctg aaactataga
      301 cggaatagcc aaagtaacta aaggtaacca taccacactg ggcgattcat gcacgtgatg
      361 aaaggaatca gaatccatac gggttttacc aaagaatact acgaatagta atcgaaacga
      421 atacaaggat gtaacaaaca cgcctgctag tactgcccaa taggcaattt ttgcagcacg
      481 caaattgcta aaggctacag attcaataat taaatcttta gaaaagaacc ctgaaaaaaa
      541 tggcatacca acaattgcca aggtgccaat taacatacac aaaaatgtta taggcatttt
      601 tcgccgtaac ccacccattt tccaaatgtt ttgctcgtga tgtactccga taatgaccga
      661 acctgcaccg agaaagagaa gtgccttaaa aaaagcatgg gtcattaaat gaaaaatccc
      721 caccgcataa ctggaaaccg ctactgcaac catcatatag cctaattgtg ataaggttga
      781 ataggcaata atgcgtttaa tatcttgatt taccagtcca ataagtccca taaaaaaggc
      841 agtgatacta ccaataatta aaataaaatt taatgccacg tctgaatgtt caaaaatggg
      901 tgacagacga gcaaccataa aaacacccgc tgtaaccatg gtggctgcat gaatcagcgc
      961 ggaaataggc gtaggacctt ccatggaatc gggtaaccaa acatgcaaag gaacttgcgc
     1021 tgatttaccc atagccccaa caaaaagcaa aatacaaatc acatgaatgg caggaaacat
     1081 tacctcatcg gaaaagataa aaaattgatt agccccgaca atcgtttcca cttggttaaa
     1141 tactgtcgtg tagtctaaag aattaaataa aaggaatacg ccggcaatac caagtaagaa
     1201 gccgaaatct cctacgcgat ttacgataaa agcttttaaa ttagcttgaa ttgcactggg
     1261 tttcttatac caaaacccga taagcagata agacataagt ccaacagctt cccaaccaaa
     1321 aaataattgc aagaaattgt tagacataac taacatcaac atggaaaatg taaaaaggga
     1381 aatgtaagaa aaaaagcgtt tatacccggg atcaccttgc atatagccta cggtatatat
     1441 gtgaaccatc aaagaaacaa aagttactat caccatcatc agcgtgctga gttcatcaat
     1501 aagaaaaccg atttgtaagc ttaggttacc cgtggttccc cagatataag cagttaaatt
     1561 aatcggggaa accgtgttgg taaattttac ggtatagagc gctaaacaag aaaacacaaa
     1621 agcaatagca acccccaaaa tagtcacgct atgtgcttga acgttgctaa tttttctgcc
     1681 taaaagaccg gcaaaacttg cagcaattaa aggtgctaat gtgatgatta cacaataata
     1741 aaatatagtt tgttgcatgt tatcccttga gcaaatctat ctcttccaca tttatggttt
     1801 gctttttgcg aaatagcaca accaaaatgg ctaaaccaat ggcagattca gctgccgcta
     1861 cggtcataac aaaaaatacg aaaatttgac catctagatt ttctaagaag tgagaaaatg
     1921 caataaagtt ggtattgata gcgagcaaaa tcagttctat acacataaga agcacaatta
     1981 aattgcgacg attgataaaa atgccggcca ttcctatccc aaaaagtatg gcgctaaaaa
     2041 tcaaataatc ggataaggaa atcataatgc ctctcctgtt tcgctaggca tttttaccat
     2101 atgcagacga tcctgctttg tcgtaaatat ttgtttttga atattttgtt ttctgcttcg
     2161 gtgtccacga aatgccaggg ctatggcggc gatcatggcc actaataaaa ttactccggc
     2221 aacttcaaaa ggtaaaatat attgggtaaa caacaattca ccgagcgctt caatgttact
     2281 ataggaagcc gaagctcgct ccgggacaat gatatagtcc aaaccaaaat ggtgtgggcc
     2341 aatcgctaaa attaatgttg ctatcatcag acataaaata aataaaccta aaggaatgtg
     2401 gcgtttaaag gacagtttta atgtgccaac ttcaatatct agcatcataa cgacaaaaag
     2461 aaaaagaacc atcaccgcac ctacatacac aagcaccaaa gcaatggcta aaaattccgc
     2521 ttctaacatt agccaaatcg cagcggatgt aaaaaaagta aggactaaaa ataacaccgc
     2581 tctgacggga ttttgagaaa aaataactcc caaactcgct gtcagcgcca ccaatccaaa
     2641 aaaataaaat atcaatgact caaacatggt agattcttat ctgtagggtg catcttcttc
     2701 tcggtttttc gcaatcgatg gttcaagttt atcgccaatt gctaataatt tttgtttgtc
     2761 taaaatattt tggccacgtt caaggattgc aaaatttaat tgatccgttt caacaatgga
     2821 gtcaaccgga catgattctt cgcaaaatcc gcaatagata catttaaata aatcaatatc
     2881 atagcgactg gtacgacgcg ttccatcttc tctttcatac gattcaatgg taatagccag
     2941 tgccgggcaa actgcttcgc ataatttgca agcaatacaa cgttcttcgc cgttgggata
     3001 tctgcgaagc gcatgaattc ctcgaaaccg tacagatacg ggtgttgttt cttcaggata
     3061 ataaatagtt attttgcgct taaataaata tttacccgtt aattttaagc ctagcaaaag
     3121 ttcccaaagc ataaaacttt ttatatattg ttttaagcgc tttaacacaa aatactatcc
     3181 tttttaaaaa tttaagcagc aaaccacgga cgaagattaa gttttatagc tacagccaat
     3241 acaacaatcc atacgagcgt aacgggtata aatatcttcc agcccaagcg cataatttga
     3301 tcatatcgaa atctcggaaa ggttcctcgg aaccacaagt ataaaaatac gagaaaagct
     3361 attttaagta ataaccaaaa aatgccagga acatacgaaa aaaactcttc tagtaaaggt
     3421 atgccttcaa aaggagataa ccaaccaccg aaaaacatga tacttgcaag agccgcaagt
     3481 agaatcatat ttgcatactc tgctaaaaag aataaagcaa aagtcatgcc tgaatattca
     3541 acatgaaagc ctgctactat ttctgactca ccttccgcca catcgaaagg attacgattg
     3601 gtttctgcta cagccgaaat ccagtaaatc acgcataacg gtaaaagcgg cagccaatac
     3661 caatggatta tgcttccttg ttgcgcaagc acaatattgg ttaaattcat actgccggat
     3721 gccatcaaca ctcctaccaa acaaaatccc attgcgattt cataagaaac gacttgcgcg
     3781 gcagatcgca tagcacctaa aaatgcatat ttagaattag aagcccagcc ggcaataatg
     3841 attccgtaca cacccatcga tgtcatagct aaaagaaata aaagtccaat atttaaattt
     3901 gccaaaacta agccatagtc aaaaggaatc accgaccacg caatcaaagc gggggctacg
     3961 gttagtatgg gaccaacgat atataaaaat ttgtttgcat gcgtaggtaa aattatctct
     4021 ttaaataata atttaattgc atctgctatg ggttgtaata atccataata ccctacgcga
     4081 ttaggaccaa cacgacactg gatataacct aaaactttac gttccacaaa tgttaaatag
     4141 gctacactca gcatcaccgg cacaataatg agtataattt tgagtaagat ccaacctacc
     4201 gcaaatagtg tatcgaacat atcctatccc actcgttcta aggttatcac gctgtaggga
     4261 gcacctagca gggtttcttc aaaacctgca taaagcaaaa tggatccttt cggtacatta
     4321 gggttaagct gaacttcgaa agcttcatgc gttattctac cttgttgttg tgcttttact
     4381 tttgcgcctt cttgaatttg cagatgatct gccgtttcgg gatgcaaccc aattttttta
     4441 ttcgattgcg tttctatagt ttcttgtaaa cattgtgctc ttcttaccaa actatcggtt
     4501 ctatacatag ccattggcgc taatcgaatt aatctatctt ttaagggagg ttcaattttt
     4561 tctggtattt tatgtaaaat ttgattctct attacgggag cttgctcgaa atttttacgt
     4621 atttcgaaag ttatttcttt tgctttgtta tattgaaaac ctggcaattt gaacaaattg
     4681 gctaataccc tcaatatttt ccatgcaggt ttcgaattgc catacggttt agccacagct
     4741 ttaaatgatt gccaatctga tgtcatatta atgaatgtac catcggtttc gctacttaac
     4801 gccataggca ataaaatact ggcatacgac cgcatccggt cggtaacaaa aggtgttatg
     4861 catataactt cttggacttt tgttaaagtt tctaagctta acgctggatg atatgcatca
     4921 aattctggct cgaaattcat taataccatg cttgccagag gctcgtgtaa catttctagt
     4981 gccgttttac cggcgtttaa atcaagcgtt tccccaacag ggccacgatg gggaataaag
     5041 cctgcgatcc aggcaccact tgcattggcg cctgttggaa tttcaccaat cttggcttgc
     5101 gtaatgttgg c
//
LOCUS       DASTIB010000251         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_132360, whole genome shotgun sequence.
ACCESSION   DASTIB010000251 DASTIB010000000
VERSION     DASTIB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132360"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VFP93_04360"
     CDS             complement(<1..1017)
                     /locus_tag="VFP93_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET9843869.1"
                     /translation="MMDAKSISLKHWLKYIESIHPQQIDFGLQRIRPVAQKAALNQLG
                     CPVVLVGGTNGKGSTVALLAKLLQAMGLRVGAYFSPHLIYFTERIVLNGTTCAEAKLC
                     SAFAQVEKLRGKTPLTFFEFTTLAAFHIFKEEKLDVILLEVGCGGEKDAVNIVEPDLS
                     IITSVGLDHTEWLGDTIAEIGTAKAGILRAHKPAILGIEAQIPSILQKAQHLQSKLIL
                     EGRDFDWNDIYKSYWNYQSRFIQIPPHSIPSTSVSLALAALENLWSTFFSQKSLPYDH
                     LSYYLRTEQMPGRFQLVYTKCETIFDVAHNESGAIWLADKFKKLYPQVKTVGVWSSLS
                     DKNLE"
     gene            complement(1004..1936)
                     /gene="accD"
                     /locus_tag="VFP93_04365"
     CDS             complement(1004..1936)
                     /gene="accD"
                     /locus_tag="VFP93_04365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET9843870.1"
                     /translation="MSWFKKLVTSKIRTNTVTKKSIPEGLWTKCDACNAVLFKAELER
                     NLDVCPKCSYHKRIGGRQRLEHFLDDTIKEELGKHVEPQDRLKFKDTKRYKDRIIQAQ
                     KQTGEKDALVVMRGTLQEVPLVAAAFDFNFIGGSMGAVVGEKFVLAIDACLKHHTPFV
                     CFATSGGARMQESLISLMQMAKTAAALRKLSDAHLPYISILLDPCMGGVSASLAMLGD
                     INIAEPNALIGFSGPKVIQQTIRQTLPEGFQRSEFLLEHGVIDMIIDRRMMRDKIAGL
                     LAKLMHQPLPNRPIKPKSSMYEFSGHRELMHDGC"
     gene            complement(2008..>2262)
                     /locus_tag="VFP93_04370"
     CDS             complement(2008..>2262)
                     /locus_tag="VFP93_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9843871.1"
                     /translation="LEIVANAFLHHMVRNMVGVLLIIGRGLKPMEFAEEILHKRNRSH
                     GIMTAPPSGLYLSQVYFEGMDLPATNHYPWFLQQLSLQTF"
BASE COUNT          703 a          467 c          402 g          690 t
ORIGIN      
        1 ctctaaattt ttgtccgata aacttgacca aacacctact gtcttaactt ggggatataa
       61 ctttttaaac ttatccgcta accaaatcgc accagattca ttgtgcgcaa catcaaaaat
      121 agtttcacat ttagtataaa caagttgaaa acgacctggc atttgttctg tgcgcaaata
      181 ataacttaaa tgatcataag gtaatgattt ttgactaaaa aatgtactcc atagattttc
      241 aagcgcggca agagcaagag atacactcgt agaaggtatg gaatgcggag gaatctgtat
      301 aaaacgcgat tgataattcc aataactttt ataaatatca ttccaatcaa aatctcgacc
      361 ttctaagata agtttgcttt gcaaatgttg cgctttttgt aaaatggaag gaatttgggc
      421 ttctatacct aaaattgcag gcttatgcgc tcgtaaaatt cctgctttgg cagtacctat
      481 ttcggcaatc gtatccccta accactccgt atgatccaat cccacggaag taataatact
      541 taaatctggc tcgacaatat ttacagcatc cttctcgcca ccacacccta cttctaacaa
      601 aatgacgtct aatttttctt ctttaaagat atgaaaggcg gcaagcgttg taaattcaaa
      661 aaaagttaaa ggagttttac cacgaagttt ttcaacttgc gcaaaagccg aacaaagttt
      721 tgcctcagca caagttgtcc cgtttaaaac tatacgctcc gtaaaataaa ttaaatgggg
      781 tgagaaatag gcacctacgc gtaatcccat tgcttgcaac aatttcgcta acaacgcgac
      841 cgtagagcct ttaccattgg ttccacctac taacacaact ggacagccca gttgattcaa
      901 tgcagctttt tgtgccacag ggcgaatacg ttgtaaacca aagtcgattt gttgaggatg
      961 aatggattct atatatttga gccaatgctt tagagaaatc gatttagcat ccatcatgca
     1021 taagttccct atgtcctgaa aattcataca tagaagattt gggttttata ggacgattgg
     1081 gtaagggttg atgcataagt tttgcaagta atcctgcaat tttatcgcgc atcattcttc
     1141 gatcaatgat catatcgatt acaccatgct ctaataaaaa ttcagaacgt tgaaaacctt
     1201 cgggcaacgt ttgtcgaatc gtttgttgaa ttactttagg gcccgaaaaa ccaatcaagg
     1261 cgttgggttc tgctatattg atatctccga gcattgccag acttgcagaa acccccccca
     1321 tgcatggatc caataatatc gaaatataag gtaaatgggc atcactcaat tttcttaaag
     1381 cggcagcagt ttttgccatt tgcataagtg atattaagga ttcttgcatg cgagccccac
     1441 cactggtggc aaagcaaaca aaaggagtat ggtgtttaag acatgcatcg attgctaaaa
     1501 caaatttttc acctacgaca gctcccatgg agccaccaat aaaattaaag tcaaacgcag
     1561 cagcaactaa aggaacttcc tgtaaagtac ctcgcataac caccaaggca tccttttctc
     1621 ccgtttgttt ttgtgcttga attatgcgat ctttataacg ttttgtgtct ttaaacttta
     1681 agcgatcttg tggctctaca tgtttgccaa gttcttcttt aatggtatcg tctaaaaaat
     1741 gttctaatcg ttgacgtcca ccaatgcgtt tatgatagct acattttgga cagacatcta
     1801 aatttctttc taattctgcc ttaaataata ctgcgttaca tgcatcgcac ttcgtccata
     1861 gtccttctgg aatagatttc tttgtgacag tatttgtgcg aatttttgaa gtaacgagct
     1921 ttttaaacca actcatcgag caccatcgtc aagtagagtg ggttcattat aactctattc
     1981 gttatctcac ataatattcg atacaactta gaaagtttgc agacttaatt gttgaaggaa
     2041 ccaaggataa tggtttgttg cgggtaagtc catcccttcg aaatatactt gacttaagta
     2101 taaacctgaa ggaggtgcag tcattatacc atgcgatcga ttacgtttat gcaatatttc
     2161 ttcagcaaat tccataggct ttaagccgcg tccaattatt aaaagaacac ctaccatgtt
     2221 gcgtaccatg tgatgcaaaa aagcattggc gacaatttcc aa
//
LOCUS       DASTIB010000252         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_456012, whole genome shotgun sequence.
ACCESSION   DASTIB010000252 DASTIB010000000
VERSION     DASTIB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456012"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VFP93_04375"
     CDS             complement(<1..1262)
                     /locus_tag="VFP93_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843872.1"
                     /translation="MQLPWHINKLTLEFFLAHIHNDDQQVDKLIYYLKHASIEDIVAT
                     VCLNDRSEKYKNSLLEQIISVSKYQALLKIILERIPGHYLDFSNMKYYKLKKLVTLLI
                     KDKSSFYLDKLKNTPSFDLNAICDPYYQTTVLSEIVRATERNVDVLLKILATFSLEKV
                     NFNAAEKNTLNKDTLLFKLFSIAVSKNKPHIIDIILNSVPFRHLDFTNASVKAWPPTS
                     ENMIQWLNTTEFRDYIPKIIESAITQGKIFWDRLIHTADQDGNVDEVQNKIFEDHLEK
                     IKLFAEHSQSITCATRLEELQDYRNAKIYELHSDVENLEILANKISTVHRFYEEIYNI
                     LAESIAFSMHITEKPIELDPKAHEKQLLRALNCMLDASRDAELKKHIALTYVFRSLNG
                     GIPSAEKGTLIQLITKTKIPVSESGKKT"
     gene            complement(1359..1643)
                     /locus_tag="VFP93_04380"
     CDS             complement(1359..1643)
                     /locus_tag="VFP93_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843873.1"
                     /translation="MRDQNLEPENHKQTLMEDKASISVPCQTLPIPIKNPITSQFNRV
                     KQEATLKNNLEDSASQKHPMQMYLRERDELKKNTIGSPPGSRLKYNPRQI"
     gene            2262..2876
                     /locus_tag="VFP93_04385"
     CDS             2262..2876
                     /locus_tag="VFP93_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HET9843874.1"
                     /translation="MTSTSLIHGFSLTASLIVAIGAQNAFVLKQGLKKHHLLFTASLC
                     ILFDCILIALGVCGLGVFIEKYPAFTLWFRIFGASFLLFYGIKAFSSALNPQSYTLEL
                     HFQPTTRLRTFALLVTFTFLNPHTYLDTIVLIGAAGAQYEVNEQILFILGAMCASTLW
                     FFSLTFGAKKLSSHFQKPKTWQVLDFFVGVLMLFLAFSFVYPLL"
     gene            2941..>3656
                     /locus_tag="VFP93_04390"
     CDS             2941..>3656
                     /locus_tag="VFP93_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9843875.1"
                     /translation="MAQHIRTPLLYSHPLSEIVGKQVFLKLELLQPSGSFKDRGIGNL
                     CMHFAQKNDKGFIASSGGNAGMSVAFAGKKLKRKTTIIVPKTTSHMMIQKIRHLHAQV
                     IVHGDNWNEADELALQKVEEEKLNYIPPFDHPRIWQGYESLIDEFVQDNYKPDVIILS
                     VGGGGLFTGIVQGLVKHGWNAIPIITVEPEGAATLAKAIEKKEHITLTEIKTIAGTLG
                     AKKIANEAYLWCQKWDVLPQ"
BASE COUNT         1086 a          631 c          622 g         1317 t
ORIGIN      
        1 atagtttttt ttccactttc cgaaacaggt attttagttt ttgttattaa ttgaatcaat
       61 gtcccttttt ctgcggatgg aatccctccg ttcaaagatc gaaaaacata ggttaaggca
      121 atgtgtttct taagttcagc atctctactt gcatctaaca tgcaattaag cgcgcgtaaa
      181 agttgttttt catgcgcttt cgggtctaat tcgatgggtt tttccgtgat atgcatacta
      241 aaagctatcg attctgccaa tatattataa atttcctcat aaaatcgatg cacggtggag
      301 attttattgg ccaatatctc taaattctca acatccgaat gcaactcata aattttggca
      361 ttcctataat cttgtaattc ctctaacctt gttgcgcagg taatactttg tgaatgttcc
      421 gcaaagagct ttattttttc taaatgatct tcaaatattt tattctgcac ttcatccaca
      481 ttcccatcct ggtcggcagt atgtataaga cgatcccaaa atatttttcc ctgtgtaatt
      541 gcagattcta taatttttgg aatgtaatca cgaaattctg tggtatttaa ccactgaatc
      601 atattttcgc ttgttggggg ccacgctttc acagaagcat ttgtaaaatc taaatgtcgg
      661 aaaggtacgc tatttagtat aatatcaata atgtgtggct tatttttact taccgcaatg
      721 ctaaatagct taaacaataa agtatcttta tttagtgtat ttttctctgc tgcattaaaa
      781 ttaacttttt caagtgaaaa tgttgcgagt atctttaaca aaacatccac attcctctct
      841 gtggcgcgca cgatctctga caagacggta gtttggtaat aaggatcgca aattgcattc
      901 aaatcaaaac ttggtgtatt ttttaactta tccaaataga aagaactttt atccttaatt
      961 agtaaggtta ccaatttttt taatttataa tatttcatat tagaaaaatc taaatagtga
     1021 ccgggtatac gttccagaat tatttttaaa agcgcttggt acttacttac gcttataatt
     1081 tgttctaaca gtgaattctt atatttttca gagcgatcgt ttaaacacac cgtggcaacg
     1141 atatcttcaa tagaagcgtg ttttagataa taaattagtt tatcaacttg ttgatcatcg
     1201 ttatgaatat gtgctagaaa aaactctaag gttagtttat taatatgcca aggaagttgc
     1261 accgtaactc caccactatt tttttacttg tgaacatacc ataaaatatt catagtgcca
     1321 aagttgcatt tgattttatg aaatagttta atttaccttt aaatctgacg cggattgtac
     1381 tttagccttg aacccggtgg actgccaatt gtattttttt taagttcatc acgctctctt
     1441 aaatacatct gcatgggatg tttttgggat gctgaatctt cgagattatt ttttaaagtg
     1501 gcttcttgct tcactctatt aaattgagag gttataggat ttttgatggg tatcggaagg
     1561 gtttggcacg gaacacttat gcttgcttta tcttccatga gagtttgctt gtggttttcc
     1621 ggttcgagat tttggtcgcg catcatctac tccattcata atctccttct tttagttttc
     1681 ccataaatat gccattcgca caactgattt ttttagcttt ttagcaaaag taagatgaat
     1741 caatatatct ttttacttat ttccttaact ttattaaaca atacagatat acttggatta
     1801 tccacaataa gtgtggataa aggtggggat gtgttgcgaa cagttgttta ataagtcatt
     1861 ttatcaaaat atttcaataa cttatcaaac tgttcactat ttaaccaaga gaaatattta
     1921 tgagtactat ttttttactc tcgacagtgc cagttatatt acttttcttc tttaaaaaca
     1981 acatgagtag aaaagtcagt ctttattgta cactattctc attccctttc tttctttttt
     2041 tgctacattt taccttagcc gtgatttttg gccttgtcgc atttcttgtt tttgcgtttt
     2101 taatcaaaaa aacttttcgc aaaaaaaata tgcaatatct aaacatatat gggcgaaaga
     2161 ttaatcttta accttttatc gggcgataat taacttcata tcccgattgg ttttcaaaaa
     2221 aaagtatttt aaaatatttg aagttttata ataagtgctt tatgacatcc acttcgttaa
     2281 ttcacggttt tagtctcact gccagtttga ttgtagctat tggggcacaa aatgcctttg
     2341 tattaaagca aggattaaaa aagcatcatt tactatttac agcttcactt tgtattttat
     2401 ttgattgtat tttaattgca ttaggagtat gtggtttagg tgtatttatc gaaaagtatc
     2461 cagcatttac actgtggttt cggatctttg gagcaagttt cctattattt tatgggatca
     2521 aagctttttc atcggcttta aatccgcaaa gttacacact tgaactccat tttcagccta
     2581 cgacccgtct gcgtacgttt gctttgcttg tgacttttac ttttttaaat ccacatacct
     2641 atttagatac cattgtgctc attggtgcag cgggcgcgca atacgaagta aatgaacaaa
     2701 ttttatttat cctcggtgcc atgtgtgctt ctacactttg gttttttagc ttaacctttg
     2761 gtgcaaaaaa attatcgtcg cattttcaaa aacctaaaac gtggcaagtc cttgattttt
     2821 ttgtcggtgt gttaatgctt tttttggctt tttcatttgt ataccctctt ctttaaaaaa
     2881 cgctagccaa atttttaaat tagctctacg ataaattgca atgcaatacg taagagaatt
     2941 atggcacagc atattcgtac accactttta tattctcacc cattaagtga gatagttggc
     3001 aaacaagttt ttttaaagct ggaattactg caacctagtg ggtctttcaa agatcgtggc
     3061 attggcaact tatgcatgca cttcgcacaa aaaaatgaca aaggttttat tgcctcatcc
     3121 ggcggaaatg cgggcatgag tgttgcattc gctggaaaaa agctaaaacg gaaaactaca
     3181 attattgtgc ccaaaacaac ttcacacatg atgatacaga aaattcgtca tttacatgcg
     3241 caagtaatcg tacatggaga taattggaac gaagcggatg aacttgcact tcaaaaggtt
     3301 gaagaagaaa aattaaacta tattccaccc tttgatcacc cacgcatttg gcaaggttat
     3361 gagtctttaa tagatgagtt cgttcaggat aattataaac cggatgtaat tattttgtct
     3421 gtgggcggcg ggggtctttt tacgggtatt gtgcaaggtt tagtaaaaca tggctggaat
     3481 gctattccaa taattactgt agaacctgaa ggcgctgcaa ccttagcaaa agcaatagaa
     3541 aaaaaagaac atatcacatt gacagagata aaaacgattg caggaacgct tggagccaaa
     3601 aagattgcaa atgaggctta tctgtggtgt caaaaatggg atgttctacc gcaaat
//
LOCUS       DASTIB010000253         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_902079, whole genome shotgun sequence.
ACCESSION   DASTIB010000253 DASTIB010000000
VERSION     DASTIB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902079"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VFP93_04395"
     CDS             <1..1337
                     /locus_tag="VFP93_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843876.1"
                     /translation="PDKRMCIMMNPELRRNIWLEFTTHRLIAAPVVLGCILYIGYLTG
                     GMPYLKKISLQLFILLSFVWGTRTCSDGICSEINAHTWDWQRMSSLSPMKMSIGKLLG
                     STSFTWYCSLLVFMAYIACSTVLNGTPVLTSALVLFMGALFTQSLALVFSLNVLHYER
                     HEKILSFKYFVAAVFIGGNLTYYAYKSLNLTFLVPWFHYNFSVNYFAPISLTFFFLWS
                     LIALYRNLRIELQYKNIPWVWGAFTVFCVVYFSGFLYQPNLHIVPPGKWFKTLNFGSL
                     FFAFAVAMFLTYLSFLMEEIHLLRYKKLIISWSKGKISFLENLPRWVVSFIIMLFIGI
                     WIMLKPNDTAFRIDLFVMALTLLSIRDILLAHFFNFGINRKRTMLTILFYFAVLYILI
                     PTLLELMHLKPVIAFFIPAIEDYPQYSIMGPLFQVSIFGYLVMLRAKRLNQN"
     gene            complement(1334..>2530)
                     /locus_tag="VFP93_04400"
     CDS             complement(1334..>2530)
                     /locus_tag="VFP93_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9843877.1"
                     /translation="RKEEEKNYLILHCAGEGIFGIDAQNKITFVNPAAENMLGWKYRE
                     VHGQPFQGILQPATLDGTKLAIEDSPIYKTYQDGIVREVNNERFIRKSGVAFDVEYIA
                     RPLRDLHGALRGVVVTFKDITRRRELEKTLAKTNQELVTLSRYAGISEVSSGVLHNLG
                     NILNNVNTSFHWIKEKLIELKIKKIILALEQLKNSKVDTNKRKQFQEYIHSALLADQK
                     IIADSIQESKIVEDLITHAKNVLHTQQNFTKKITYKEEVSVEQLIQEAVALSYLGSDL
                     RITYLIKDNPFVLVDKHKAIQILINLIKNAYNAMKEYSDQKPELLFQCEKRNGRIRIK
                     IQDRGPGIPPEVAPNLFAFGVTSRKEGHGFGLHNSANLATEMQGRLFLESSTPAGTCF
                     VLEIPF"
BASE COUNT          700 a          459 c          425 g          946 t
ORIGIN      
        1 aaccagacaa aaggatgtgc ataatgatga atcctgaact aagacgtaac atttggttag
       61 aattcaccac acaccgactc attgcagccc ccgttgttct cggttgtatt ttgtacattg
      121 ggtatttaac cggcggtatg ccttatctta aaaaaatatc tttacaactt tttattttat
      181 tgagttttgt atggggtaca cgtacgtgtt cggatgggat ctgctcggaa atcaatgcac
      241 acacgtggga ttggcaaaga atgtcttcct taagccctat gaagatgagc attggaaaat
      301 tattgggtag tacttcattt acctggtatt gttctttact cgtttttatg gcctatatag
      361 cgtgtagcac ggttctcaat ggaactccgg tattaacctc tgcactcgtt cttttcatgg
      421 gcgctttatt tacgcaatct ttagcgcttg tatttagcct taatgtacta cactatgagc
      481 gacatgaaaa aatactttca ttcaaatatt ttgttgcggc tgtatttatt gggggtaatc
      541 ttacctatta cgcatataaa tcattaaatc ttacgttttt agtcccatgg tttcattata
      601 atttttccgt aaattatttt gcacctattt cattaacatt ctttttccta tggagcttaa
      661 tcgctttata tcgtaatttg cgtattgagt tacaatataa aaatattcca tgggtttggg
      721 gggcatttac tgtattctgc gtggtctatt ttagtggatt tctttatcaa cccaatttgc
      781 atattgtccc accagggaaa tggtttaaaa cgttaaattt tggtagtttg ttttttgcat
      841 ttgctgtagc aatgttttta acatatctca gttttcttat ggaagaaatt catcttttac
      901 ggtacaagaa acttattata agttggtcaa aaggtaaaat aagcttttta gaaaatttac
      961 caagatgggt tgtttcattt attattatgt tattcattgg gatctggatc atgctaaaac
     1021 ccaatgatac tgcattccgg atcgatttat ttgtcatggc acttacgctt ttatctattc
     1081 gcgatatatt actcgcacat ttttttaact tcggtatcaa tcgaaagcgt actatgttaa
     1141 ccattctttt ctattttgct gtactttata tattgattcc aactttattg gagttgatgc
     1201 atctaaaacc agttatagct ttttttatac ctgcaattga ggattatccc caatattcca
     1261 ttatgggccc cttgtttcaa gtctctattt tcggttattt ggtcatgttg cgggcaaaac
     1321 ggttgaacca aaattaaaaa ggaatttcta aaacaaaaca agtacctgca ggggtgctcg
     1381 attccaagaa taaacgcccc tgcatttctg tcgcaagatt tgcagaatta tgcaaaccaa
     1441 aaccatgccc ctctttgcga ctcgtgacgc caaaagcaaa taaatttggg gcaacttccg
     1501 gagggatccc aggcccccta tcttgtattt ttatgcgtat tctgccattc cgcttttcac
     1561 attgaaaaag gagctctggt ttttggtccg aatattcttt cattgcatta tatgcatttt
     1621 ttattaaatt gattaaaatt tgaattgctt tgtgcttatc gaccaaaaca aatggattgt
     1681 cttttataag gtaagttatg cgtaaatctg atccaagata ggataatgca acggcttcct
     1741 gtatgagttg ttccacagaa acttcttctt tatacgtaat ttttttagta aaattttgtt
     1801 gtgtatgtag tacatttttt gcatgtgtta ttagatcctc aacaattttc gattcttgta
     1861 tagaatccgc aataattttt tgatctgcca ataaagcgct atggatatac tcttgaaatt
     1921 gttttctctt attcgtatcg actttcgagt ttttaagttg ttcaagtgca agaatgattt
     1981 tcttgatttt caattctatt aatttttctt taatccagtg gaatgaagta ttaacattat
     2041 ttaaaatatt gcctaaatta tgcaatacgc cactcgaaac ttcggaaata cctgcatagc
     2101 gcgaaagtgt caccaattct tgattagttt tcgctaatgt tttttctaat tctcttcttc
     2161 tagtaatatc tttaaaggta accacaacac cacgcaaagc tccatgtaag tcacgtaaag
     2221 gccttgcaat gtattctaca tcaaatgcaa caccactttt acgtataaaa cgttcgttat
     2281 taacttcgcg tacgatacca tcttgatagg ttttatatat gggagaatct tctattgcca
     2341 atttggtacc atcgagcgtt gcaggctgca agatgccttg aaaaggttga ccatgtactt
     2401 ctcgatattt ccatcctaac atgttttcag cagctggatt tacgaaggtt attttatttt
     2461 gcgcatcaat cccaaaaatt ccttcaccag cacaatgtaa aattaaataa tttttttctt
     2521 cttcttttct
//
LOCUS       DASTIB010000254         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_913927, whole genome shotgun sequence.
ACCESSION   DASTIB010000254 DASTIB010000000
VERSION     DASTIB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913927"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2785
                     /locus_tag="VFP93_04405"
     CDS             <1..2785
                     /locus_tag="VFP93_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9843878.1"
                     /translation="DRVSGVIDDLPEEADPPEVQKTSADDQPILWLNLNSKTMSVMEL
                     TDYARRYLLDKFSSLDGVARVRIGGAKDKAMRIWLNRHEMAARNLTVQDIEQALRTEN
                     VELPAGQLHSKSRDFTVRMQRMFTSPDDFTKLVVARGERGHLVRLGDIAKVEVAPAEE
                     RGFFRGNGAAMVGLGIIKQSRANTLDVANSIKKRVTEVATTLPPGLNLDLSYDSSVFI
                     ESAIKEVYLTLFIAVFLVILVIYLFLGNLRATLIPAITVPVSLIGTFIILHILGYTIN
                     LLTLLALVLAIGLVVDDAIVVLENIYRRIELGEPRLLAAYRGANQVGFAVLAITLVLI
                     AVFIPITFLEGDVGRLFSEFAVAMAASVAFSCFVALTLSPMLASKIIQEKSKHNKLTI
                     WIDKNFERLRKIYRNSVHYFLLKPWPIMLTFFLTLLLIALLVQKIPGEFAPKEDRGVI
                     FLMINAPEGSSYEYTKKSMDVLEQKLMPFVESKEFELLLLRAPQALATTQSFNAGIGI
                     IVLSRWETGRKPIWFYIDKIMQVTSDMPGVNVFPVVRQALGGSFGKPVQFVIGGATYE
                     ELVQWRDILIEKARQNPKLVGLDYDYKETKPQLLVRINTDKAGDLGVSLIDINAALET
                     MLGSKRVTTFIDRGEEYDVLVEGIRNLQQSPTDMTNIYVRSKHSQALIPLSSIVTIEE
                     FADAASLQRYNKLRSITIDANLAPDYPLSEALNYLENLVHTELPPGVDIGYKGESLDY
                     KESSGGIYLIFALSLIVVFLVMAGQFESFVHPFVIMFTVPLAVFGALLGLWFFGLSLN
                     IYSQIGLIMLVGLASKNGILMVEFINQLRDEGFETIQAILDASSIRLRPIIMTSFTVI
                     MGAVPLILAHGAGAESRYVLGIVIFFGVLISLPLTLYIIPIVYLFIAKNTQSPKIIQE
                     KLESLLSDYKKVI"
     gene            complement(2821..>3872)
                     /locus_tag="VFP93_04410"
     CDS             complement(2821..>3872)
                     /locus_tag="VFP93_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843879.1"
                     /translation="LCKRIMLSSNQKVAMTVVKVIAPVLKNGLELHNGLEFDNGLEYA
                     NGFDNFENSLSKNDFINQFFEEFTLCCLEDPIFEFPFSEIIDKCFNYPTFKGSYTEIM
                     EKHQFDSTLKLPILPREIIDKYKSNHENSALNTIKTDYVQKLWAPKKIDWSIIPTPKR
                     NKPIRENSTTPNEINKETVQPNLAIAQSTSKPSETKFARTRGNTHNEKMRKFVPQKND
                     SGIINTEPLSDPYTESSLSSDSITQSDSMEQDVNINEKIIALLGIDYFQECSDMQSKK
                     RRRQTDRKRHRPHSWNNADKTPLAQNNGNNEVKGAVAESNCELKKPSLLNAFQNLRLK
                     RNHETKMHKRGMSNN"
BASE COUNT         1080 a          670 c          808 g         1314 t
ORIGIN      
        1 ggatcgcgta tctggagtca tagacgattt accagaagag gcggatcctc ctgaagtgca
       61 aaaaactagc gcagacgatc aacctatttt atggcttaat ttaaacagca aaacaatgtc
      121 tgttatggaa ttaaccgatt atgctagacg ttacttgctc gataaatttt catctttaga
      181 cggggtagct agagtaagaa tcgggggtgc caaagataaa gctatgcgaa tttggctgaa
      241 tcgacacgaa atggcagcac gcaatctaac agttcaagat atagagcagg cactgcgcac
      301 ggaaaatgtt gaattgccag cgggtcaatt acattctaaa tcacgtgatt ttacggttcg
      361 catgcagcga atgtttacct ctcccgatga tttcaccaaa ttggtcgtag ctcgaggcga
      421 aagaggtcat ctggttcgat tgggtgatat cgctaaggta gaagttgcgc ccgcggaaga
      481 gcgtggcttt tttcgtggaa atggcgctgc tatggtgggg ctagggataa ttaaacaatc
      541 gcgcgcgaac actctcgatg ttgcgaactc aataaagaaa agagttacag aagtcgctac
      601 aactttaccc cctggtttga atttagattt aagttacgat agcagcgtat ttatcgaaag
      661 tgctattaaa gaagtgtatt taacattatt tattgccgtt tttttagtaa ttctcgttat
      721 ttatttattt ctaggaaacc tgcgcgcaac cttaattcct gctattacgg ttccagtttc
      781 tttaatagga acctttataa ttctacatat tttaggctac accattaatt tattaacttt
      841 actagcttta gtattggcaa ttggtttggt agtagacgat gctattgttg tcttggaaaa
      901 tatttatcgc agaattgaat taggtgaacc gcgcttgctt gcggcctatc gaggcgcaaa
      961 tcaagtaggt tttgcagttc tggcgattac attagttctg attgctgtat ttattccgat
     1021 taccttttta gaaggtgatg taggacgttt attttcagaa ttcgcggtgg ctatggcggc
     1081 atcggttgca ttttcttgtt ttgttgcctt aacgctttct cccatgttgg cgtcgaaaat
     1141 catccaagaa aaaagtaaac acaataaact taccatctgg attgataaaa attttgaaag
     1201 attgcgtaaa atttatcgaa atagcgttca ctatttttta ttgaagcctt ggccgattat
     1261 gctcactttc tttttaacct tactgctaat tgctttatta gtacaaaaaa ttcctggaga
     1321 attcgcaccc aaagaagatc gaggcgttat atttttaatg attaatgcgc ccgaaggctc
     1381 ctcgtatgaa tacactaaaa aaagcatgga tgttttagaa cagaagttaa tgccatttgt
     1441 ggaatcgaaa gaattcgaac ttctcttatt acgtgctcca caagccttag ctacaaccca
     1501 gtcatttaat gcaggtatcg gtattattgt gcttagtcga tgggaaacag gcagaaaacc
     1561 catttggttt tatatagaca aaattatgca agtaacatca gatatgcctg gtgttaatgt
     1621 ttttcccgtc gttagacagg ctttaggagg aagtttcggt aagccagtac aatttgttat
     1681 aggaggcgca acctatgaag agttagtaca gtggcgagac atcttgatag agaaagcacg
     1741 gcaaaatccg aaacttgtag gcttagatta tgattataaa gaaacgaaac cacaactttt
     1801 ggttcgaatt aatacggata aagcgggcga tttaggagtt tcgttaattg atatcaatgc
     1861 tgccttagag actatgctag gttctaaacg ggttaccact tttatcgatc gaggtgaaga
     1921 atatgatgtt ttggtagagg gaatacgtaa tttgcaacaa agtcctaccg atatgaccaa
     1981 tatttacgtg cgttctaaac attcgcaagc acttatacca ctttctagca ttgtaactat
     2041 agaagaattt gcggatgcag cttccttgca acgctataat aaattaagaa gtataacgat
     2101 cgatgctaat ttggctcctg attatccact tagcgaagcg ctcaattatt tggaaaatct
     2161 tgtgcacact gagttgccgc cgggtgttga tattggctac aaaggtgaat ctttggacta
     2221 caaagaatcg agcggcggaa tttacctaat ttttgcttta tcgttaattg tggttttttt
     2281 agtgatggcg gggcaattcg aaagttttgt gcacccattc gttattatgt ttacggttcc
     2341 tttagcagta tttggtgcgt tgcttggtct ttggtttttc gggttgagct taaatattta
     2401 tagccagata ggattgatta tgctagtggg acttgcctct aagaatggga tcctcatggt
     2461 tgaatttatt aaccaattgc gtgatgaagg ttttgaaaca attcaagcta ttttagatgc
     2521 ttccagtatt cgactacgtc ccattattat gacgagtttc acggtaatta tgggagcagt
     2581 acctttaatt ttagcccatg gcgcaggggc agaatcgcgt tatgtattag gcatcgtaat
     2641 attttttggc gtgctcatat cgttgccgct cacactttac atcatcccta ttgtttattt
     2701 atttatcgct aaaaatacac aatcaccaaa aattattcaa gaaaaactag aatctctttt
     2761 aagtgattac aaaaaagtca tataagaaac ccttcgagca gagtttctta taacttgttt
     2821 ttaattattc gacattcctc ttttgtgcat tttggtctcg tggttgcgtt ttaaccgtag
     2881 attttgaaat gcgtttagta aggatggttt ttttaattca cagtttgatt ctgctacagc
     2941 tcctttaact tcattatttc cattgttttg cgcgagcggg gttttatctg cattattcca
     3001 agagtgcggt ctatggcgtt tacgatccgt ttggcggcgt cgttttttag actgcatatc
     3061 actacattct tggaaataat ctatgcctag aagtgcaata atcttttcat ttatattgac
     3121 atcttgctcc atactatcag actgcgtaat agaatcgctg gacaaagagg attccgtata
     3181 tgggtctgac aaaggctctg tgttaattat cccagagtca tttttttgag ggacaaattt
     3241 acgcattttt tcattatgtg tgttaccacg tgtgcgagcg aatttcgttt cacttggctt
     3301 actcgtactc tgcgcaattg ctaagtttgg ttgcaccgtt tctttgttta tttcatttgg
     3361 ggtagtgcta ttttcgcgga tgggtttatt tcgttttgga gtaggaataa tggaccaatc
     3421 tatttttttg ggagcccata atttttgcac gtaatcagtt ttaattgtat tcagggctga
     3481 gttttcatga ttggatttgt acttgtctat aatttctctg ggcaatatag gtagttttaa
     3541 cgttgaatca aattgatgtt tttccataat ttccgtgtat gatcctttaa aggtaggata
     3601 gttaaaacac ttgtctataa tttcagagaa gggaaattca aatattggat cttctaagca
     3661 acataaagtg aattcttcaa aaaattggtt aataaaatca ttctttgaca gggagttttc
     3721 gaagttatca aatccgttag cgtattcaag gccgttatcg aattcaagtc cgttatgcaa
     3781 ttcaagcccg ttttttaata ctggagctat aaccttaaca acagtcattg caaccttttg
     3841 attactcgaa agcatgattc ttttgcaaag gt
//
LOCUS       DASTIB010000255         4091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1222453, whole genome shotgun sequence.
ACCESSION   DASTIB010000255 DASTIB010000000
VERSION     DASTIB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222453"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1167
                     /locus_tag="VFP93_04415"
     CDS             85..1167
                     /locus_tag="VFP93_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET9843880.1"
                     /translation="MYPILIAGAGNIGKLIACLLAESGSYKVYLSDINLSHLPKNALG
                     TQKSNIEFIEFDATDKAMFGQIVGKTQIKAVVCALPYFCNVAVAESAREHHLHYFDLT
                     EDTYASEKVSELAANADAAFVPQCGLAPGFVSIVANEYMHRFDKIQDVLMRVGALPQH
                     ANNQLKYSLTWSTEGVINEYGNPCYALVGGETVPLQPLEGLEQVEIDGMVYEAFNTSG
                     GLGTLVFSFKHKVDTMNYKTLRYPGHCHQMKFLMNDLKLNEDRSTLQNILENALGKTK
                     QDVVIIYVAVKGIQNEELMEETFVKKIYPQKIAGKMWSAIQVTTASSLCAVVDKITQE
                     SPKGLVLQESIPLAAITDNRFGQCYR"
     gene            1179..2702
                     /locus_tag="VFP93_04420"
     CDS             1179..2702
                     /locus_tag="VFP93_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9843881.1"
                     /translation="MDVFAELGLKQVNAGTSLGDGHFLQSSLNSPPAIHSYNPTTESL
                     LGSIHATSLPEYEKVIEHSQQSFTWWRMTPAPKRGEFVRAMGEALRKKKDALGTLVSL
                     EMGKSKQEGDGEVQEMIDMADFAVGQSRMLYGLTMHSERPHHRMYEHWQPLGLVGVIS
                     AFNFPVAVWSWNAFLAAICGNTVIWKPSSKVGLCAIAVQNIANEVMQAMNCKGVFSLI
                     IPQDHDLAEHFVEDKRIPLISFTGSTKVGRKVGQQVASRLGRSLLELGGNNAIIIDET
                     ADLNLAIPAVLFGSVGTAGQRCTSTRRLFIKDSIFDEVMEKLIHAYKQVRIGDPLDRS
                     NLMGPLIDQSAVEQYQNTLQEIRKLGGEIVFGGNVLSRTGYFVEPTLVIADHDWPIIQ
                     HETFAPILYLIKYADFKEAITWHNAVPQGLSSAIFTNNLRNAEKFLSAQGSDCGIANV
                     NIGTSGAEIGGAFGGEKETGGGRESGSDAWKYYMRRQTTTINWGDTLPLAQGIKFEL"
     gene            complement(2686..3882)
                     /locus_tag="VFP93_04425"
     CDS             complement(2686..3882)
                     /locus_tag="VFP93_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HET9843882.1"
                     /translation="MKNRMPSVWLLIFIIGLPIFSETAYSTALVDIAKALSTSENMVE
                     HTLSTYLFGFALGTLIWGNLSDKIGRKPCLLGGYSIYILACLCCYFSPTIEILLLSRF
                     IQAFGGSVGSVIGLAICRDSFTDYNKGKIFSTITSTVAFVPALGPIIGGWVARSFGWP
                     AIFLLLLMIGLAIFCISCLQLPETYKPTLARNTFWNNLRRMLRDPKLMAFCCMVAGCN
                     GIMFSYFSEGAFYCIDYLNTSEKTFSYTFLGIAFSAFLGGRLSRWLHNWNTSFTLLKK
                     GAYTTCLGTMLFSGVAIGTLFLPVSSALHLTFYMITLCITLFGIGLMIPNVLNIALED
                     YQDNIGTASAIFGCGYYLLITLLIFILATLHNDTIFPLPFYQFCIAMGMVLIITLSTF
                     KPTIQT"
BASE COUNT         1369 a          758 c          849 g         1115 t
ORIGIN      
        1 catagtagca tcaaacgaga tcttatttgg tagtactcag cagattcaaa gtagttggaa
       61 aattaaataa aggagtatga atacatgtat ccaattctta tagcgggagc tggtaatatt
      121 ggaaaattaa ttgcatgttt attggctgaa tctggaagtt ataaagttta tctctctgat
      181 attaatttat cccatctacc aaaaaatgct ttaggaacac aaaaaagcaa cattgaattc
      241 attgagttcg atgctaccga taaggctatg tttggccaaa ttgtaggaaa aactcaaatt
      301 aaagccgtgg tttgcgcttt accttatttt tgcaatgtag cggtcgctga aagtgccaga
      361 gagcaccatt tgcattattt tgatctcacc gaagacacct atgcatcgga aaaagtaagt
      421 gagcttgcgg caaacgctga tgccgctttt gtgcctcaat gcgggctagc acccggtttt
      481 gttagtatcg tagccaacga atatatgcat cgttttgata aaatccaaga tgtattaatg
      541 cgagtcggtg cgttaccaca acatgcgaac aatcaattga aatattcttt gacatggtct
      601 accgaagggg taatcaacga atacggtaac ccgtgttatg ccttagttgg aggcgaaact
      661 gttcctttgc aacccttgga aggcttagag caagtggaaa ttgatggaat ggtgtatgaa
      721 gcttttaata cgtccggtgg attaggcacc ttagtgttta gttttaaaca taaagtagac
      781 acaatgaatt ataaaacgtt acggtatcct ggtcattgtc accaaatgaa atttctcatg
      841 aacgatttaa aattaaacga agatcgctca accttacaaa atattttaga aaatgcttta
      901 ggcaaaacta agcaagacgt tgtaattatt tatgtcgcag tcaaaggcat acaaaatgaa
      961 gaacttatgg aagaaacctt tgtgaaaaag atttatccgc aaaaaattgc aggcaaaatg
     1021 tggtcggcca ttcaagttac gaccgcgagc agtttatgtg cggtggtaga taaaatcact
     1081 caagaatccc cgaaaggttt agttttacaa gaatctatcc ccttagccgc tattaccgac
     1141 aatcgatttg gccaatgtta tcgctaggag tataaagaat ggatgttttt gcagaattag
     1201 gtttaaagca agtaaatgca ggcacaagtt taggcgacgg acattttcta caaagcagtt
     1261 taaattcacc acctgcaatc cattcttaca atcccacaac cgagagcttg ttaggctcca
     1321 ttcacgcgac cagtttgcca gaatatgaaa aggtcataga acattcccaa caaagcttta
     1381 catggtggcg tatgaccccg gctcctaagc gcggagagtt tgtacgtgct atgggtgagg
     1441 ctctgcgaaa gaaaaaagac gcgttaggta ctttagtatc tttagaaatg ggcaaaagta
     1501 agcaagaggg cgatggcgaa gtgcaagaga tgatcgacat ggccgatttt gcagttggac
     1561 aatcgcgcat gctctatgga ctaacaatgc attcggagcg cccgcatcat cggatgtatg
     1621 agcattggca gcccttaggt ctggtcgggg taattagtgc attcaatttt ccggtagcgg
     1681 tttggtcgtg gaatgcattt ttagccgcca tttgtggcaa tacagttatt tggaaacctt
     1741 cctccaaagt aggactctgt gcaatcgctg tacaaaatat cgccaatgaa gtcatgcaag
     1801 ccatgaattg taaaggtgtt ttttctctta ttattcccca agatcatgat ctggccgagc
     1861 actttgtgga agataaacgt atccccctca tttcttttac tggctctacg aaagtaggta
     1921 gaaaagtagg acagcaagtt gctagtaggc tgggacgatc acttctagaa ttaggtggaa
     1981 ataatgcaat tataattgat gaaaccgcag atttaaattt agctattcct gcggtacttt
     2041 ttggttcagt tggaactgcc ggacaacgtt gcaccagtac gcggcgtctt tttattaaag
     2101 atagtatatt tgacgaagtc atggaaaaat tgattcacgc ctataaacaa gtgcgtattg
     2161 gcgatccatt agatcgttct aaccttatgg gcccgcttat tgatcaaagt gcagtggagc
     2221 aatatcaaaa taccttacag gaaatacgca aattgggtgg tgaaatcgtt tttggcggaa
     2281 atgtattatc ccgaacaggc tattttgtag agccaaccct tgtgattgct gatcacgatt
     2341 ggccaataat acaacacgag acatttgcgc caattttata tttaattaaa tatgctgatt
     2401 tcaaagaggc cattacctgg cacaatgccg ttcctcaagg attatcctcg gcaattttta
     2461 ccaataacct aagaaatgca gaaaagtttt tatccgctca gggcagtgat tgtggaatcg
     2521 ctaatgtaaa tattggaaca tcgggcgcag aaataggagg tgcttttggc ggcgagaaag
     2581 aaaccggagg tggaagagaa tccggctctg atgcttggaa atattatatg cgacgtcaaa
     2641 ccactaccat caattggggc gatacattac ctttagccca aggcattaag tttgaattgt
     2701 aggtttaaac gtagaaagcg ttattatgag aaccattccc attgcgatac aaaactgata
     2761 aaaaggcaaa ggaaaaatgg tatcgttatg tagagttgcc aaaataaaaa tcaacaaagt
     2821 tataagcaaa tagtaaccgc atccaaaaat ggccgaagca gttccgatat tatcttggta
     2881 atcttctaag gcgatgttta gcacatttgg gatcataagg ccaattccaa ataaagttat
     2941 gcatagcgtg atcatataaa aagttaagtg aagggcggaa gaaacaggca aaaaaagcgt
     3001 tccgatcgct acgccagaaa aaagcatagt acccaagcac gtagtataag cacctttttt
     3061 taagagtgtg aaagaagtat tccaattatg cagccaacgc gataatctgc cgcctaaaaa
     3121 ggctgaaaat gctataccta agaaagtgta actaaaagtt ttttccgaag tatttaaata
     3181 atcaatacaa taaaatgcgc cttcagaaaa atagctaaac ataataccat tgcaaccagc
     3241 aaccatacaa caaaatgcca taagcttagg atcacgtagc attcttcgca ggttgttcca
     3301 aaatgtgttt ctagcaagtg taggcttata agtttccggt aattggaggc aagagataca
     3361 aaaaatagct aaacctatca tgagcaataa taaaaaaatc gcaggccacc caaatgagcg
     3421 tgcaacccat ccaccaataa tgggccctaa ggctggcaca aaagcaacgg tacttgtgat
     3481 cgtagaaaaa atttttcctt tgttatagtc ggtaaaagaa tctctgcaaa tggctaatcc
     3541 gataacgctt cctacactgc cgccaaaggc ttggataaaa cgactcaata ataagatttc
     3601 aatagtaggt gaaaagtagc aacataagca tgccaaaatg taaatgctat agcccccgag
     3661 caaacagggt ttgcgtccta ttttatccga taaattaccc caaataagtg tccctaaggc
     3721 aaacccaaaa agataagtgc ttaaggtgtg ttctaccata ttttcagacg ttgataatgc
     3781 tttggcaata tccaccaaag ccgtggaata ggctgtttcg gaaaatatgg gtaacccaat
     3841 aataaaaata agaagccaca cggaaggcat acgatttttc ataaaacctc gatgaaaatt
     3901 atatcaaaga ccgaaaaaaa ttatgctata aaaaaaatgt ttttcatgct aagtatctaa
     3961 acgaaatttt aacgataggt taaaaatttg atacaatagt ctacagtaac tagcttcata
     4021 agcaggtatt ttgtcatttt aatttcttat aaaacgaatc aaccagctgt ccgactataa
     4081 catagtaaat a
//
LOCUS       DASTIB010000256         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_735274, whole genome shotgun sequence.
ACCESSION   DASTIB010000256 DASTIB010000000
VERSION     DASTIB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735274"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFP93_04430"
     CDS             complement(<1..355)
                     /locus_tag="VFP93_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HET9843883.1"
                     /translation="MDITELLTFGVQNNASDLHLSAGMPPIIRVDGEMRRINLPPLDH
                     KAVQSLVYDIMNDKQRKEFEEKLETDFSFEVPGLARFRVNAFNQQRGCGAVFRTIPSK
                     VHSLKELQLPPIFQEI"
     gene            418..1122
                     /locus_tag="VFP93_04435"
     CDS             418..1122
                     /locus_tag="VFP93_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9843884.1"
                     /translation="MSVTQQWHKILKMIELYEQEFFRSKGSVTVLAVSKGQPLEKIQA
                     LHSAGQHHFGENYLVEMLTKLSALSSKNIIWHYLGNLQSKKLKKIATHCHWVHSITRI
                     KEIELLNQHIPKGTPKLNVCLQVNVQKTENKLGIGTKDLLRIAEHLLNFPKIRLRGLM
                     TFPDPSSSFEKQREPFKRLRELYEYLKDRGFLLDTLSMGTSADFKAAIAEGSTMIRLG
                     TILFGERYKGNLGAIE"
     gene            1115..1945
                     /gene="proC"
                     /locus_tag="VFP93_04440"
     CDS             1115..1945
                     /gene="proC"
                     /locus_tag="VFP93_04440"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET9843885.1"
                     /translation="MNKVTIGFIGAGNMGRSLIEGLLQSPSAPQEIFVCDHHPDRLQD
                     LIATQSITLLNAPKEVAHQSDVVVLAVKPQSMKEVLQQIQGFEVSSQPLLMSIAAGIT
                     IAQIVQFLHVSESYPIVRVMPNTPAIIKQGVSGLFAQKNLPLSYRSLAESIMLAVGKT
                     VWLSDEALMDVVTALSGSGPAYFFYFIEKLIKGAIELGLVDSTARELTLQTGIGALQM
                     ALHSEKSLSELRQQVTSKGGTTEAGIAILDQNNLETILEKALSAATRRAKELSQKLFN
                     "
     gene            2033..>2431
                     /locus_tag="VFP93_04445"
     CDS             2033..>2431
                     /locus_tag="VFP93_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET9843886.1"
                     /translation="MKKTFNVVFCLIISFLIVGTSLAQNVTPNTLTSPSGIPKNILFI
                     GPPGSGKGTQASVISKLYQIPTISTGDLLRKEALSGSHVGAQIKQTMATGMLVSDRVV
                     LELLENRLNQPDTKNGFILDGYPRTLQQAQT"
BASE COUNT          770 a          459 c          483 g          719 t
ORIGIN      
        1 caatttcttg aaatatagga ggcagttgta attcttttaa tgagtgtact ttagagggaa
       61 ttgtacgaaa tacagcacca caacctcttt gttggttaaa agcattgacc cggaatcgcg
      121 ccaatccagg aacttcgaag gaaaagtccg tttctaattt ttcttcgaac tcttttcttt
      181 gtttatcatt cattatgtca taaacgagac tttgaaccgc tttatgatca aggggaggca
      241 agttaatccg ccgcatttct ccatcgacac gaatgattgg aggcataccc gcagacaaat
      301 gcaaatcgga agcattgttt tgtacaccaa aagttaataa ctccgtaata tccatttcac
      361 actccgaaaa tatactttat ggtattatgt gtttctttat acatgcatct caacccaatg
      421 tctgtaactc aacaatggca caaaatttta aaaatgatcg aactctacga gcaagagttt
      481 tttcgctcca aaggaagtgt taccgtttta gcagtctcca aggggcaacc tctggagaaa
      541 atacaagctc tgcattctgc ggggcaacat cattttggcg aaaattatct tgtcgaaatg
      601 ctaactaagt tatcggcttt atcttctaaa aacattattt ggcactactt gggtaattta
      661 cagagtaaaa aattaaaaaa aattgcaacc cattgtcatt gggttcacag tataacgcgc
      721 ataaaagaaa ttgaattgtt aaatcagcat ataccaaagg gtacgccaaa attaaatgtg
      781 tgtttgcaag ttaatgttca gaaaactgaa aataaattgg gtataggtac taaagatctt
      841 ttgcgtatcg ctgaacatct gttaaatttc cccaagatac gtttgcgtgg cttaatgacg
      901 tttcccgatc cgagttcatc ttttgaaaag caaagagaac cttttaaaag attgcgtgaa
      961 ctctatgagt atttaaaaga tcgaggattt ttattagata cactgtccat gggaactagt
     1021 gcagacttta aagcagcgat tgcggaaggc tcaactatga tacgtcttgg aactatcctt
     1081 tttggtgaac gctataaggg aaatttaggt gcaattgaat aaggtaacaa ttggttttat
     1141 tggtgcgggc aacatgggac gtagccttat tgaagggtta ctacaatctc ctagtgctcc
     1201 tcaagaaatt ttcgtttgtg atcatcatcc cgatcggctt caagatttaa tagcaacgca
     1261 atctatcact ctgcttaatg ctcccaaaga agttgcacat caaagcgatg tggtggtttt
     1321 agccgttaaa ccgcaaagta tgaaagaagt tctgcaacaa attcaagggt ttgaggtcag
     1381 ctctcaaccg ctgttaatgt ccattgcggc gggaataacg attgcccaaa ttgtgcaatt
     1441 tttgcatgta tccgagtctt accccattgt gcgagttatg cctaacactc ctgcgattat
     1501 aaaacagggg gttagtgggc tttttgcgca aaaaaattta ccactttcct atcgatcgtt
     1561 ggcagaatct attatgttag ctgtgggaaa aaccgtgtgg ttatcagacg aagccttgat
     1621 ggatgtggta acagcattat caggatcagg acctgcttac ttcttttact ttatagaaaa
     1681 attaataaaa ggggccatag aattaggctt ggttgactct acagcaaggg agttaaccct
     1741 acaaacaggc atcggagcct tgcaaatggc gcttcattca gaaaaatcct taagtgaatt
     1801 gcgccaacaa gtgacatcga aaggcggtac gacagaggca gggatcgcta tcttagacca
     1861 aaataatctt gaaaccatat tagaaaaagc gttaagcgct gcaacacgaa gagcaaaaga
     1921 attgtcgcaa aagcttttta actgatcttt gttgtaaact atttacactg cagagcgatt
     1981 gactgtataa caatgtttgc tataatttac aattagataa aattattaga ttatgaaaaa
     2041 aacatttaac gtcgtatttt gtcttataat ttcgtttctt atcgtcggta ctagcttggc
     2101 gcaaaatgtt acaccaaaca ctttaactag cccaagtgga attccaaaga acattctttt
     2161 tattggtcca ccaggttctg gtaaaggtac gcaagcatcc gttatcagca aattgtatca
     2221 aatacctact atttcaaccg gggatttatt acgaaaagag gctttgtctg gttcgcatgt
     2281 aggagcccag attaaacaaa cgatggcaac gggtatgcta gtgtccgatc gcgtagtttt
     2341 ggaattgcta gaaaaccgcc ttaaccaacc tgatacaaaa aatggcttta tcttagatgg
     2401 ttatccaaga acattgcaac aagcacaaac t
//
LOCUS       DASTIB010000257         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1242170, whole genome shotgun sequence.
ACCESSION   DASTIB010000257 DASTIB010000000
VERSION     DASTIB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242170"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /locus_tag="VFP93_04450"
     CDS             <1..1700
                     /locus_tag="VFP93_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_process: GO:0007264 - small GTPase mediated signal
                     transduction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RasGEF domain-containing protein"
                     /protein_id="HET9843887.1"
                     /translation="LKPEEFKKASVNANQAAVVDVNKNNELKRSNENVSNPHRESTSN
                     LKQMQIWFDKITNYLIQLIFTTLPTEEMQTHLTNEEYQEKVHDHITYFLMIYIYTMDI
                     LLAENPDFNSANIVNAVLNSPAISRLKVPFMQLPDGMKTTFQSYQQYFSGANWENQRR
                     VLETNERAIPFPQIFVTDLLHSHDSSFLRTYDAKQQKSLDKVIHSQIMKTYKLGKIFN
                     FVGKIGTMQLENFPAASTDFLEEVNRTIMDRETYMQMSFKIQPPFPSVDPSEVDSAPA
                     LNPTQISNVFKWFKAHKLPLQLTINRTRQVGPYIYTPFSQYLQNKFQERFNVKWVTEC
                     SDEQIRSLSLAQIRKMSAEEIKSLSNKQIALFNINQLETFSTEQLQALTYDQVKALKQ
                     RKHAIFTKENLQALRIQNKNQSDSLLAYWDLYKEFPQPDYTLTEIERGKDNKEKEKPK
                     EYLMVNPLNLNERLRSHARKYFTKCKLNQKKSTLYTIKELKNAPGEISEDLGKSFAHL
                     TIRKSRKERNKEKSYPTKLHSESSVVNPKVVNEISFFASKDKKKERRKSKSNLHLSK"
     gene            1769..>2035
                     /locus_tag="VFP93_04455"
     CDS             1769..>2035
                     /locus_tag="VFP93_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET9843888.1"
                     /translation="MLNEQFIIERFQHDFKDLPFNTVGIGDDAAIIPIDQDECYVVTK
                     DLLIEDIHFRLHYFDAYSIAHKTLHANMSDIAAMGASPQYAILGL"
BASE COUNT          765 a          357 c          329 g          584 t
ORIGIN      
        1 agcttaaacc tgaagaattt aaaaaggcat ccgttaatgc gaatcaagcc gcggtagttg
       61 atgtaaataa aaataatgaa cttaaacgat cgaacgaaaa tgttagtaat ccccataggg
      121 aaagcacttc gaatttaaaa caaatgcaaa tctggtttga taaaatcacg aattatttaa
      181 tccaacttat atttacaacg cttcctactg aagaaatgca gacacactta acgaacgaag
      241 aatatcaaga gaaggtacat gatcatatta cctatttttt aatgatttat atttatacta
      301 tggatatttt gttggcagaa aatcccgatt ttaattcagc gaacatcgta aatgccgttc
      361 ttaacagccc agctatttct agactaaaag taccgtttat gcaattaccc gatgggatga
      421 aaaccacgtt tcaatcttat caacaatatt tttcgggtgc aaattgggaa aaccaacgaa
      481 gagttttaga aacaaacgag cgtgctatcc cttttccaca aatttttgtc accgatttat
      541 tacatagcca tgactcaagc tttttgcgta cgtatgatgc taaacaacaa aaatctttag
      601 ataaagttat ccatagtcaa attatgaaaa cctataaact cggtaaaatt ttcaattttg
      661 ttggaaaaat aggtacaatg caattggaaa attttcctgc ggcttctacg gattttttag
      721 aagaagtaaa tcgaaccatt atggatcgag aaacttacat gcaaatgtcc tttaaaatcc
      781 aaccaccctt tcctagcgta gatccaagtg aagttgattc tgctcctgcg ctgaatccta
      841 cacaaatttc taatgtgttt aaatggttta aagcacacaa actaccgctt cagttaacca
      901 taaatcgtac aagacaagta ggcccctata tatacactcc tttctcgcaa tatcttcaaa
      961 acaaattcca agagcgattt aatgtaaaat gggtaacaga atgcagcgat gaacaaattc
     1021 gaagccttag tttagcgcaa attagaaaaa tgtccgcaga agaaataaaa tctttatcca
     1081 ataagcaaat tgctttattt aatataaacc aattagaaac attcagtact gaacaacttc
     1141 aagcacttac ctatgatcag gtgaaggctt taaaacaacg taaacacgcg atttttacaa
     1201 aggagaattt acaggcttta aggattcaaa ataaaaacca atccgattct ttactggctt
     1261 attgggattt gtacaaagaa ttcccacaac cggattatac gttaaccgaa attgaaagag
     1321 gtaaagataa caaagaaaaa gaaaaaccca aagaatattt aatggttaat ccacttaatt
     1381 taaatgaaag gttgcgctca catgcaagaa aatattttac aaaatgtaaa ttaaatcaaa
     1441 aaaaatcaac gctttataca attaaggaat taaaaaacgc acccggtgaa atttctgagg
     1501 atttaggaaa atctttcgca cacttaacga tacgaaaatc gagaaaggaa agaaataaag
     1561 aaaaaagtta tcccacaaaa ctgcattctg aatcctctgt tgtcaatccg aaagtggtga
     1621 atgaaatttc gttctttgcg agtaaagaca aaaaaaagga gcgcagaaaa tcaaaatcaa
     1681 atctacattt atccaagtaa atagtaattt atttattgtt aagtaagtgc attaactctg
     1741 ttgatgcacc tctttaggaa gagagcccat gttaaatgaa caatttatta ttgagcgatt
     1801 tcaacacgac ttcaaggact tgccatttaa tacagtgggt attggcgatg atgccgctat
     1861 tatcccaatc gatcaagatg aatgctatgt ggtaaccaaa gacttattaa ttgaagatat
     1921 tcattttaga ttgcattatt ttgacgctta tagcattgcc cacaagacct tacatgcaaa
     1981 tatgagtgat atcgctgcga tgggcgctag tcctcagtat gcaattttag gatta
//
LOCUS       DASTIB010000258         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1174514, whole genome shotgun sequence.
ACCESSION   DASTIB010000258 DASTIB010000000
VERSION     DASTIB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174514"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="VFP93_04460"
     CDS             complement(<1..1747)
                     /locus_tag="VFP93_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RhoGEF domain-containing protein"
                     /protein_id="HET9843889.1"
                     /translation="MPLNEPPKLNMPFSEEELLAQRLKDERAQQLKNEQEQKLIADRN
                     ERVINAITTKIQAYIQEPTNKGFIDVVTDYLMYKAGDPISNKDIIDAFENEKNAIGKI
                     LSTMTKEMQTALLSSKKFARLKDFNPNIAKVADEVGIYEVKEKKLIIEQPRAEAPKSQ
                     LWNNLSKRLKRKSSFGKVNTTIQETTEAVAKVTEQPQEIESKTPEKPQSAFEEAESST
                     LDLVNFIDDLEKKEQKTVSILEELKATEQSFITGFLKPHEILSLSVYGSATKNTSLED
                     LARNATDIIESSKALQEVLSKVNAPEELGNILEMYKSHVQLLSQYSQNYETIQKQANP
                     EFYKFLDSFGLDPTTKLSMGSKLIVGAQRAPRYILLLTDLLKNSTTLNGDRVIKDLLA
                     ITQDAFAVFDSSKTAIKENKDTSKVEVESQNADIFEEFDKLIEDVKKPITENQDKNTN
                     EKTLVFETSTIEPPKVNDSTEALLNNAKAVAEAVQNLKKVVTPQNKEASIKETKPETI
                     LQETKKDNALNEQNVIKEQPKVNEPEAQLGISVSQTKDPTISLMENRLKTVTNDDYKD
                     LGAQILEDYTKAHQPN"
     gene            1924..>2985
                     /gene="htpG"
                     /locus_tag="VFP93_04465"
     CDS             1924..>2985
                     /gene="htpG"
                     /locus_tag="VFP93_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET9843890.1"
                     /translation="MAEAALKETRGFETEVKQLLHLMVHALYSNKEIFLRELISNASD
                     AAEKLRFEGIKDDSLYEGDVNLRIKVTYDKEARTITVTDNGIGMSKDEVIQNLGTIAK
                     SGTQAFLKSLTGDEAKDRHLIGQFGVGFYSSFIIADKVSVRTRRANLPASEGVLWEST
                     GEGEYTIETIEKQPRGTEITLHLKQGEEEFLNAMRLRHIITKYSDHISLPIVMDAESY
                     GEEKEKDEKKKQEPEEEVINKATALWTLPKADIDDEHYCEFYKHIAHDFEDPLTWAHN
                     KVEGKLEYTSLLYIPSKAPFDIWQPNKVRGLKLYVQRVFIMDDVEQFLPHYLRFVRGI
                     IDSNDLPLNISREILQHNRT"
BASE COUNT          843 a          540 c          547 g         1055 t
ORIGIN      
        1 cgtttggttg atgtgctttt gtataatctt ctaaaatttg agcacccaag tctttataat
       61 cgtcgtttgt aaccgttttt agccgatttt ccatcaagga aatagtagga tcttttgttt
      121 gcgatacgga aatcccaagc tgtgcttctg gttcattaac cttgggttgc tcttttatta
      181 cattttgctc gtttaaagca ttgtcctttt tagtttcctg gagaatagtt tctggcttgg
      241 tttctttaat tgaagcctct ttgttctgag gtgttaccac tttttttaga ttttgcaccg
      301 cttcggctac tgctttagca ttattgagta atgcttctgt tgaatcatta acttttggag
      361 gctcaatagt agaagtttcg aataccaatg tcttttcgtt agtattttta tcttgatttt
      421 ctgtgatggg ctttttaaca tcttcaatta gtttatcgaa ttcttcaaaa atatctgcat
      481 tttgagactc aacttctact ttggaagtgt ctttattttc tttaatggct gtttttgaac
      541 tatcgaaaac agcaaatgca tcttgtgtga tggcaagcaa atccttaatt accctatcac
      601 catttagagt ggttgaattt ttaagcaaat ccgttaataa caagatataa cgtggagctc
      661 gctgcgcacc aacaattaac ttcgatccca tactaagttt tgtggttgga tccagtccaa
      721 agctgtctaa aaacttgtag aattctggat tcgcttgttt ttgaatagtt tcgtaatttt
      781 gcgaatattg ggatagtaac tgcacatgac ttttgtacat ttctaaaata ttacctaatt
      841 cttcgggtgc gttaactttt gaaagtactt cttgcaatgc cttcgatgac tcaattatgt
      901 cggtagcatt tcgagctaaa tcttctaagc tagtattttt agtcgcggat ccataaacac
      961 ttaaagaaag aatttcgtgt ggttttaaaa accctgtgat aaaactttgt tcggtagctt
     1021 tgagttcttc taaaatactt acagttttct gttctttttt ttcaagatcg tctataaagt
     1081 taacaagatc taaagtagaa ctttctgcct cctcgaaagc tgattgtggc ttttctggcg
     1141 tcttagattc gatttcttga ggttgttcag ttactttggc gacagcttca gtagtttctt
     1201 gaattgttgt atttactttt ccaaaggaag actttctctt aagtctttta ctcaaattgt
     1261 tccacagttg cgattttgga gcttcagctc ggggctgttc aataataagt ttcttttctt
     1321 taacttcgta gatgccaact tcatccgcca ctttagcaat attaggattg aaatctttta
     1381 atcttgcaaa tttcttggaa gagagtagtg ctgtttgcat ttcttttgtc atggtgctta
     1441 gaatttttcc aatagcattt ttctcatttt caaatgcatc tattatatct ttattgctta
     1501 tgggatctcc tgctttgtac attagataat cagtcactac atcaataaaa cctttattag
     1561 tgggttcctg tatgtaggct tgtatttttg ttgttattgc atttattacc ctttcattgc
     1621 gatcagcaat taatttttgt tcttgttcat tctttaactg ctgtgcacgc tcatctttta
     1681 agcgttgcgc aagcaattct tcttcactga agggcatgtt tagttttggt ggttcattaa
     1741 gaggcattct cgcacctaaa atgagtacta ctcattacat tgtatcaagc caactaacta
     1801 aatcatatag tttatatatc cagcaaacgt gtagttatta agttctaaac ccttgaaatt
     1861 cataagaatg cccctatatc atgtttaacc tttcattttt tccaaattta ccaaaaggtg
     1921 tatatggcag aagcagcttt aaaagaaaca cggggttttg aaactgaagt gaagcaattg
     1981 ctccatttaa tggtgcatgc actgtattca aacaaagaaa tttttttacg agaacttatt
     2041 tctaacgcat cggatgctgc agaaaagtta cgttttgaag ggatcaaaga cgatagcctt
     2101 tacgaagggg atgtaaacct tcgcatcaaa gttacttatg ataaagaagc gcgtactatc
     2161 accgtaaccg acaatggcat tgggatgtca aaagacgagg tgattcaaaa tttaggtact
     2221 attgccaaat cgggtaccca agcattttta aagtcgctta caggcgatga agccaaagat
     2281 cgccacctca taggtcagtt tggggtaggt ttttattcaa gctttattat tgccgacaaa
     2341 gtaagcgtac gcacccgtcg tgctaatctt ccagctagcg agggagttct ttgggaatcc
     2401 acaggtgagg gcgaatacac cattgaaacc atcgaaaaac aaccacgcgg tactgaaata
     2461 acgttgcatt taaaacaagg cgaagaagaa tttctaaacg cgatgcgttt gcgccatata
     2521 attaccaaat attctgatca tatttcatta cccattgtga tggatgcaga atcatacggc
     2581 gaagaaaaag aaaaggatga aaagaaaaaa caagaacctg aagaagaagt catcaataaa
     2641 gccaccgctt tgtggacttt accaaaagcc gatatagacg atgaacatta ttgtgaattt
     2701 tataaacaca tcgcacacga cttcgaagat cctttaacgt gggcgcataa taaagtagaa
     2761 ggcaagcttg aatatacttc ccttttatat attccttcga aagcgccatt cgatatttgg
     2821 caacccaata aagtgcgtgg gttaaaatta tatgtgcagc gcgtatttat tatggacgat
     2881 gttgaacaat ttttacccca ttatttacgc tttgttcgag gtattatcga ttctaacgat
     2941 ctcccgttaa atatttcgcg tgaaatttta caacataatc gtacg
//
LOCUS       DASTIB010000259         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1035900, whole genome shotgun sequence.
ACCESSION   DASTIB010000259 DASTIB010000000
VERSION     DASTIB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035900"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFP93_04470"
     CDS             <1..466
                     /locus_tag="VFP93_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9843891.1"
                     /translation="ETLQKVTDDVGRRYTFNTAIAAIMELINDLTSFTSNTEIDKSIK
                     HETLEIVLQMLSPIVPHITHHLWHALGHESTIADAVWPQVDLEALIKESIELVIQVNG
                     KVRGKLEVPIHADQKAIESAVIENDNVKKHIEGKNIKKIIIIPNKLINIVAH"
     gene            466..966
                     /gene="lptE"
                     /locus_tag="VFP93_04475"
     CDS             466..966
                     /gene="lptE"
                     /locus_tag="VFP93_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET9843892.1"
                     /translation="MRVTYFITILAISLLFGCGFHLRGTHILDQTQLPFRIVTIEPYA
                     PEDRLQQDLALRLESYAVEIAKEPTPWILQWEPLLLETSPIAFGPNGELVREKLRLTL
                     RYRLKHDNSVVAEKTLWTERQHHLNVGSQLATQEERNAILKEMQTDLLAQMVLQLSKI
                     QYANNL"
     gene            950..1945
                     /gene="holA"
                     /locus_tag="VFP93_04480"
     CDS             950..1945
                     /gene="holA"
                     /locus_tag="VFP93_04480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET9843893.1"
                     /translation="MQIIYDNQLKSYLTPLAKCYILVGPEAFLIQESALQIRKAAALE
                     GFHERLLFTLEQVQTFAWNSLITTLKTPSLFQTKQIIDWFWHQIPQEKNKSDIESLFP
                     LYDNQILIIRAGYLTKAQMQSTWFQTIAQTAIVVNHWTPKAHALHFWIKERAQSKELI
                     LSQEIVQLLAEQYEGQLFALDQLFTKLRLEKSKTITLAHIQEHLALGEHPLGSLQSAL
                     LGQNPKKILEVLIYFKHTHLEMGLILWALHRLLQGLRIGHHKPNASHLLKSLGIFPKD
                     AQSFWKLVRINSPSKLEDYLQTLADIDKLHKTGAQQESWQKICDLALQIGGMYFH"
     gene            2099..2443
                     /gene="rsfS"
                     /locus_tag="VFP93_04485"
     CDS             2099..2443
                     /gene="rsfS"
                     /locus_tag="VFP93_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9843894.1"
                     /translation="MTPKQLLDLVLVDLDDLKAVNISPLDVRNMTPIADFLVIATGNS
                     SRHVASLCENLVRKMKARNITPLGVQGDKDEEWVLLDLGDVVVHIMQPRIRDFYNLEK
                     LWTFEVPQAANA"
     gene            2444..2908
                     /gene="rlmH"
                     /locus_tag="VFP93_04490"
     CDS             2444..2908
                     /gene="rlmH"
                     /locus_tag="VFP93_04490"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET9843895.1"
                     /translation="MALKIVAIGELTGWCELAFKDYQKRIRPPFTLECFQIPVSKRKE
                     KTPQKAVQEEAQKLLRHTQVRDFVFALDPNGQTISSVQFSQKINDLQNHWSNIVFLIG
                     GPDGFDSSIYNRANALLSLSSFIFPHTLARLMLIEQLYRAYTLASNHPYHRA"
     gene            2989..>4635
                     /gene="mrdA"
                     /locus_tag="VFP93_04495"
     CDS             2989..>4635
                     /gene="mrdA"
                     /locus_tag="VFP93_04495"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9843896.1"
                     /translation="MRPHLIKNQHLEKKLYLNRVFFILGIVILAFLTLLIRLSFLQIF
                     HHSRYASMSRSNVIRFEAIPPTRGLIYDRFGRVLAENITVYRLEIVPEKVKDLDQLLK
                     NIGQTIFLPEHDIMEFKKRAKRSPSFAPIPIKLRLTEKEVAILSVKKHKLPGVSISAG
                     MIRHYPYGDLFAHVVGYVGRVNERELRKLDKTRYVNTHFICKTGIEKFYENNLHGIPG
                     FREVETDAYGRPIRYLTTKPPEHGKNLVLTIDLTLQQRAIQTLQGKRGAIVALDPYTG
                     EVLALVSQPGFDPNLFVTGIDNETFNLLKNAQDKPLFNRFLKGQYPPASTIKPIFGLA
                     GLEKNIINPKTTIHDPGWYQLHEDSRVYRDWREHGHGFTNLHKAIKESCDTFYYILSE
                     KLGIDAMALWLKQFGFGEITGIDLPDENAGILPSRQWKQRTIGQSWYRGETLITGIGQ
                     GYMLSTPLQLAQSVALIANHGQKIQLHLLKEKKLSSVSQSLPIQKKNWDAIIEAMEAV
                     VHQPGGTAFQISAGLKYPMAAKTGTAQVFRLPDKKEELLLK"
BASE COUNT         1532 a          894 c          858 g         1351 t
ORIGIN      
        1 tgaaacatta caaaaagtta cggatgatgt aggcaggcgt tataccttta ataccgctat
       61 tgcagcaatc atggaattaa taaatgattt aaccagtttt acaagtaata cagaaataga
      121 taaaagtata aaacatgaaa cgcttgagat tgttttacaa atgttatcgc ccattgtgcc
      181 acacattact catcatttat ggcacgcttt aggtcatgaa agtacgattg cagatgctgt
      241 ctggccacaa gtagatttag aagcacttat taaagaatct atcgaactcg ttattcaagt
      301 taatggaaaa gttcgaggta aattagaagt gccaatccat gcagaccaaa aagccataga
      361 aagtgcggta atagaaaatg acaatgtaaa aaaacatatt gagggcaaaa atattaaaaa
      421 aattattatt atccccaata aattaattaa tattgtagcc cactaatgcg cgttacgtac
      481 tttatcacga ttttggccat ttctttattg tttggatgtg ggttccattt gcgtggcacc
      541 catatcctag atcaaaccca attacctttt cgcattgtta cgatagaacc ttatgcgccc
      601 gaagataggc tccaacaaga tttagcgctg cgattagaat cgtatgctgt tgaaattgca
      661 aaagaaccta ctccctggat attacaatgg gaacctttac tcttagaaac ctctcccatt
      721 gcttttggtc ccaatggaga gctggttcga gaaaagttac gattaacttt gcgctaccga
      781 cttaagcatg ataatagcgt tgtagcagaa aaaactcttt ggactgaacg ccaacatcat
      841 ttaaatgttg gatcacaact cgcaacgcaa gaagaaagaa atgctatcct aaaagagatg
      901 cagacagatt tgcttgcgca aatggtttta cagctaagca aaattcaata tgcaaataat
      961 ttatgataat caattaaaat cttatctaac ccctttggct aagtgctata ttctcgtagg
     1021 accagaggct tttttaattc aagaatctgc tttgcaaatt aggaaagcag ccgctttaga
     1081 gggttttcac gaacgactgc tttttactct cgagcaagtg caaacttttg catggaattc
     1141 ccttattaca actttaaaaa ccccctctct gtttcaaaca aagcaaatta ttgattggtt
     1201 ctggcaccag atcccgcaag aaaaaaataa atccgatata gaatcacttt tccctctcta
     1261 tgataatcaa atcctcatta tccgtgcagg gtatttaacc aaagcccaaa tgcaatcgac
     1321 ttggtttcaa acaatagcgc aaaccgctat cgtagtaaat cactggactc ctaaagcaca
     1381 tgctttgcat ttttggatta aagagcgcgc acaatcgaaa gaacttatcc tctctcaaga
     1441 aatcgtgcaa ttactagcag agcaatatga gggacaacta tttgctctcg atcaactatt
     1501 tacaaaattg cgtttagaaa aatccaaaac gatcacgctg gcacatatac aagaacattt
     1561 agcgctaggt gaacacccat taggttcttt gcaaagtgct cttttaggac aaaatcccaa
     1621 aaaaattcta gaagttctta tttattttaa gcatacccat ttagaaatgg gcttaattct
     1681 ttgggcatta caccgattgt tacaagggtt aagaatcggt catcataagc ccaatgcatc
     1741 acatttgtta aaaagtttag gaatttttcc caaagacgca caaagttttt ggaaactagt
     1801 gcgtataaac tcccctagta agttagaaga ttacttgcaa accttggcag atattgataa
     1861 attgcacaaa accggagcgc aacaagaaag ctggcaaaaa atatgtgatc ttgctctcca
     1921 aataggtgga atgtatttcc actgatcttt accgacaaat cacacaaaaa aatttcaact
     1981 tggaacgatt agggttatat ttaaagaagg aagcgcggta aaaataggag gatatacata
     2041 ttctgttaac ccaaaaatgg tgcacaccat ccaaaggtaa ctaacttaaa ggaaaatcat
     2101 gacaccaaaa cagttattag atttagtcct ggtcgattta gatgatctca aagccgtaaa
     2161 tatctcccca cttgatgtaa gaaatatgac gcccatagcg gattttctag tcattgctac
     2221 cggaaattct tctagacacg ttgcttcctt atgcgagaat ttagtgcgca aaatgaaagc
     2281 aaggaacatt acaccgttgg gcgttcaagg cgacaaagat gaagagtggg tcttactaga
     2341 tttaggcgat gttgtcgttc atataatgca accccgtata cgtgattttt acaatctcga
     2401 aaaactttgg acctttgaag tgcctcaagc tgcaaacgca taaatggcat taaaaattgt
     2461 tgctataggc gagctcaccg gttggtgtga gctcgctttt aaagattatc aaaaaagaat
     2521 tcgcccccct tttaccctcg aatgttttca aatacccgta agtaagcgaa aagaaaaaac
     2581 gcctcaaaag gcggtacaag aagaagcgca gaagttgttg cgtcacaccc aagtgcgcga
     2641 ttttgttttt gctttagacc caaatggtca aacaatttct agtgtgcaat tttctcagaa
     2701 aattaatgat ttacaaaatc attggagcaa tatagtattt ttaattgggg gacctgatgg
     2761 gtttgattct tccatttata acagggcaaa tgcattactt tcgctttctt ctttcatatt
     2821 tccacatact cttgcccgcc taatgcttat tgagcaactg taccgtgcct atactttagc
     2881 ctcaaatcat ccttaccata gagcataatt tcaaatgtta tttgattaac ccctgacttt
     2941 agatttaata atataataat aaggtttttt gtcgacaagg atgttggcat gcgcccacat
     3001 ctcatcaaaa atcaacatct agaaaaaaaa ctgtatttaa atcgagtttt ttttattctc
     3061 ggtattgtta ttcttgcttt tctcaccctt ttgatacggt taagtttctt acaaatattt
     3121 catcattccc gttatgcttc gatgtcgaga agcaatgtta ttcgatttga agctatcccc
     3181 cctacccgag gactcatcta cgatcggttt ggtcgtgtgc tagccgaaaa tattacggta
     3241 tatcggctag aaattgttcc cgagaaagtt aaagatttag atcagctgtt gaaaaatata
     3301 ggtcaaacaa tttttctgcc agaacacgat attatggaat ttaagaagcg agcaaaaaga
     3361 agtccaagtt ttgctcctat ccccattaaa ttgcgcttga cggagaaaga agtagccata
     3421 ctttctgtaa aaaaacataa actccccgga gtatctatta gtgctggaat gattcgtcac
     3481 tacccttacg gagatttatt tgcacatgtt gtgggttatg tgggacgagt aaatgaacgt
     3541 gagttaagga agttggacaa aactcgatac gtcaacaccc attttatctg taaaacgggt
     3601 attgaaaaat tttatgaaaa taatcttcac gggataccag ggtttagaga agtggaaacc
     3661 gatgcgtatg gccgaccaat acgatattta acaacaaaac cgccagaaca tggtaaaaat
     3721 ttagttttaa cgattgactt aactttacag caaagagcaa ttcaaacttt acaaggcaaa
     3781 cgcggtgcta ttgtggcact tgatccatat acgggtgaag tactggcgtt ggtgagccaa
     3841 cccggttttg atcccaatct ttttgtaacg ggtattgata acgaaacatt taatcttctt
     3901 aaaaatgcac aagataaacc tctctttaat cgatttttaa agggtcaata tccgccagct
     3961 tccaccataa aacctatttt tggcttggcc ggtttagaaa aaaatattat caatcccaaa
     4021 actactatcc atgatcccgg ttggtatcaa ctgcatgaag attccagagt gtaccgtgat
     4081 tggcgtgaac atggccatgg atttacgaac ttacataaag ccataaagga atcgtgcgac
     4141 accttctatt atattttgtc cgaaaagttg ggtattgatg ccatggcatt gtggttgaaa
     4201 caatttggat tcggagaaat tactggaatt gatttgcctg atgaaaatgc aggaatatta
     4261 ccttcgcgtc agtggaaaca acgcaccata ggacaaagtt ggtatcgtgg cgaaacgctt
     4321 attacaggaa ttggtcaagg ttatatgctt tccacacctt tacaacttgc gcaaagtgta
     4381 gcgcttattg ctaatcatgg tcagaaaata caactccatc tattgaaaga aaaaaagctt
     4441 tcaagcgttt ctcagtcttt accgatacaa aaaaaaaatt gggacgctat tattgaggct
     4501 atggaagctg tggtacacca accaggcggc actgcttttc aaatatctgc aggattaaaa
     4561 tatccgatgg ccgcaaaaac aggaacggcg caagtttttc gtttaccgga taaaaaagaa
     4621 gaacttttat taaaa
//
LOCUS       DASTIB010000260         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_332903, whole genome shotgun sequence.
ACCESSION   DASTIB010000260 DASTIB010000000
VERSION     DASTIB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332903"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1136)
                     /locus_tag="VFP93_04500"
     CDS             complement(84..1136)
                     /locus_tag="VFP93_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843897.1"
                     /translation="MIEWLNQNVYQNPRSLIPSLGLITTGSLALLSGIPLLTAGIIAL
                     SASGVSYLFVRAVQRVNGLLDTLEGTLEAAKKAAEDVDELKKEIVKDGKIDKLNTIEK
                     NIVEATALLKIALKEVVEKNPDTKQNKIDQISQVVKKFDSLVEKFEKNKNIEKFTEME
                     NNLIDITSNIKGLMLEIREKNPTTQKTKVEEVGDILHKLNMTVEQIKNITHNITDVNP
                     ETQKSNMDDLGDILHKISFITDKIKKITSDITDKNTETEKSKVDELVGTIDKLNGILD
                     AIRADAKIEKIGLIEKSAFETLTNVNGAIDDFRTGGVTFLMRGNGRSAPATNNASTGQ
                     NDDANNNTKSWFGWGN"
     gene            complement(1314..1862)
                     /locus_tag="VFP93_04505"
     CDS             complement(1314..1862)
                     /locus_tag="VFP93_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843898.1"
                     /translation="MAVAIVDPYPNWYTPLKWLIPLALSLSAGIGMGLLILNVVSVPV
                     AFGQLAWLPALYASMTGMKALTFLALSCGLVGTFTGVISAFLLRSTVLFFMNEYRGTQ
                     GQKAQERINELEDTIAQMAEARNAMEQEMRLLQHQVHNNELQNKNVHFQYPNFNDSQK
                     TTVNLEPTQNINTPVQTKQGLS"
BASE COUNT          672 a          431 c          357 g          789 t
ORIGIN      
        1 ggtctcgtaa agttcagcat tatttgcaat atttattaga aaagaaaaaa atgtaaatac
       61 ccgactcaag agtcgggttg catttaattt ccccagccaa accaactttt tgtattgttg
      121 ttggcatcat cattttgccc ggtagaagca ttatttgtag cgggagcgct tcttccattg
      181 ccacgcatca aaaatgttac acccccggta cgaaaatcat cgatggctcc gttcacattg
      241 gttaatgttt caaaagcact cttttcaata agtcctattt tttcaatttt tgcatcagct
      301 cgaatagcat ccaaaatgcc atttaattta tcaattgtac cgaccaactc atccaccttg
      361 gatttttctg tttctgtatt tttatcggta atatcgctcg ttattttttt gattttatcc
      421 gttatgaaag aaattttatg taatatatct ccaagatcat ccatgtttga tttttgcgtt
      481 tcaggattta catcggtaat gttatgcgta atatttttta tttgttctac tgtcatattt
      541 aatttatgaa gaatatctcc cacttcttct actttcgttt tttgggtagt aggatttttt
      601 tccctgattt caagcatcag acctttgatg ttactggtaa tatcaattaa attattttcc
      661 atttccgtaa atttttcgat atttttattt ttctcaaatt tttctactag agaatcaaat
      721 ttcttaacaa cttgggatat ttgatctatt ttattttgtt tagtatcggg attcttttcc
      781 accacttcct ttaatgcaat tttcaaaagc gcggttgctt ctacaatatt tttttcaata
      841 gtattgagtt tatcgatttt tccatcttta acaatttctt tttttaattc atcgacatct
      901 tctgctgctt ttttagcagc ttctaaagtt ccttccaagg tatctaataa tccattgaca
      961 cgctgaaccg cacgcacgaa taaatagcta actcccgagg cacttaaagc gataatacct
     1021 gcagttaaca acggaattcc ggaaagcaac gctaaactac ctgtagtgat taggcccaaa
     1081 cttgggatta aggagcgtgg attctggtaa acattttgat taagccattc aatcatataa
     1141 acctcttctt agattataac tgcgcatata cctaagacta tttggtaacc gcgctacttg
     1201 cactgcacca ttctaacagt taaggtaaac aatcgtaagc aagtaatcac tcaggaacta
     1261 ccgtatatcg gctcccactg atatttagtc gattaactga tgcgttttaa attttacgat
     1321 aatccttgtt ttgtctgcac gggagtattt atattttggg taggctctag atttaccgta
     1381 gttttttggg aatcattaaa atttgggtat tgaaaatgta cgtttttatt ttgtagttcg
     1441 ttattatgta cttgatgttg aagaaggcgc atttcttgct ccattgcgtt gcgtgcctct
     1501 gccatttgcg caatagtgtc ttctaattcg ttgatacgtt cttgggcttt ttgtccttgt
     1561 gttcctcgat actcattcat aaagaataat acagtagatc gtaataaaaa agcacttatc
     1621 acgccggtaa aggtacctac gagaccacaa gaaagcgcca aaaaggttaa tgctttcata
     1681 ccagtcatag atgcatacaa ggctggcaac catgctaatt gtccaaaagc cacaggtaca
     1741 gaaacaacat tcaaaattaa aagacccatc cctatccccg cacttaaaga tagagctaag
     1801 ggaatcaacc attttaaagg ggtataccaa ttgggatacg gatcaactat tgctacggcc
     1861 atcttaaatt cctttatatg ggtttgttgc taatattatt cacaaaattg tgagaattgc
     1921 attataaatt ttgcctacca tgataatggt ataattttta aaagctttac tgtccaacaa
     1981 atatacaaaa agctaaacac taaaaaattc caaaagataa ttcattgaaa taaaaacaat
     2041 ttttaaatgg catgctgatt gcattttaca taagtgtcaa ctgaaaaatc catccatgtt
     2101 aactcacccc acgtatggtg gggtttttta taatattttg gttcctcgtc caaagatcca
     2161 cacgaacaga caagcaaagg cgctggaact gaccatccac agtaaattat caagcgtagc
     2221 atcattaaat aatgtcacaa tatatgtcg
//
LOCUS       DASTIB010000261         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1059019, whole genome shotgun sequence.
ACCESSION   DASTIB010000261 DASTIB010000000
VERSION     DASTIB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059019"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1129
                     /locus_tag="VFP93_04510"
     CDS             167..1129
                     /locus_tag="VFP93_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET9843899.1"
                     /translation="MVLQFAVDANGILKEDKKNLEDPLWIDVCQPTSQDRIDLLEKLQ
                     VILPMHHELHQLEYSNRFYEEENNIYFSVNVITKALPIPESHVMTFILTKNTLVTLRY
                     ADPHPIRTFIDQLEKRSIHIQNPLDIFYILVEACVGRIADMFEVFDEKTNQLAQHLSS
                     IIKRTTKIKRNEALNQTLGEINDLENLLSKCFQSLSSLQFLIGFLKQVAPKIKLKIAN
                     GWPILNQDLQAMIDHGEYLTDKLSFLLESTLGLINIEQTNIIKSFTVLAMIFMPPTLI
                     ASVYGMNFRYMPEINLVWGYPFAMGVMIISAILPTVFFKLKGWI"
     gene            complement(1130..1723)
                     /locus_tag="VFP93_04515"
     CDS             complement(1130..1723)
                     /locus_tag="VFP93_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HET9843900.1"
                     /translation="MLNEYRGLSKIIFGSRWLQAPLYVGLIFAQCVYVYQFMIELYLL
                     IKDANHHNEAHVMLHVLGLIDVVMIANLIIMVIVGGYETFVTRIGIQHHPDLPEWLDH
                     VNSGTLKIKLSTALIGISSIHLLKTFINAENIEPQVMLWQVVIHLTFVISAILLAITD
                     KIMNNTSIKSRKHSIIDAKRWRKTLETPSNPKESFKM"
     gene            2001..3227
                     /locus_tag="VFP93_04520"
     CDS             2001..3227
                     /locus_tag="VFP93_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9843901.1"
                     /translation="MVRSNNFLRNASMWLLASLFYAYQFVLRVLPNIMMPDLMRQFNI
                     DASIFGQLSGVYYIGYAGMHIPIGILLDKFGPKTILPVCMVLAACGLLPFVYAQTWIY
                     PCLGRFIIGVSSSAAILGLFKIIRMAYREELFTRMLGASVTIGLLGAIYGGYPVRWLL
                     ENIGWYTTFHLLILVGVVMAVITFIIVPSIKNPTSTSVWTDLKNLFLNRKIVAVCFLA
                     GCMVGPMEGFADGWSTIFLTQIYHLGNAIAASLPSMIYLGMCIGAPLLSLLAEKTKAY
                     YEIIAMSAFIMCASFILLLAGSLQLSFVSLVFFIIGLLCAYQILAIYKASSYAKESNV
                     GITTAFANMIIMFFGYVFHTAIGKVAVHFWDGTVLQGIPFYSTFALRAALGIIPIMLF
                     VGLLGFSTLAIQEKRT"
     gene            3329..>3897
                     /locus_tag="VFP93_04525"
     CDS             3329..>3897
                     /locus_tag="VFP93_04525"
                     /inference="COORDINATES: protein motif:HMM:NF023645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HET9843902.1"
                     /translation="MELRNFNNKPRRVGFEIEYIGIPIRESANLIHKLYGGKIVEKNE
                     NLIIVETPWGPFQLEVDVYLLEKLAAASKKNIEEKKIDVQGAAQYILSNLLTAFMPNE
                     IVCPPIFAQDIEKLEPLIGNLREHGAKGTSASFAYAFGLHINVEPYSLETSMLLQYLR
                     AFVLLYDYLKETMHIDITRAITLFAKPYSL"
BASE COUNT         1190 a          665 c          725 g         1317 t
ORIGIN      
        1 atcaagcgat tccactccat gcacaagaag caatgcttct accttgggcg ttggatagtg
       61 ttttcgaatt atcgacagat cattcgcctt ttttaagtga cgtccaaggc ttacaaatta
      121 ttttgcaaca acaacttgaa aatatttaat tgagataagg atttttatgg ttttacaatt
      181 tgccgtggac gcaaatggta tacttaagga agacaagaaa aacttggaag atccattatg
      241 gatcgatgtg tgtcaaccca cgtcgcaaga tagaatagat cttttggaaa aattacaagt
      301 gattttaccc atgcatcatg agttgcatca actagaatat tccaatcgat tttatgaaga
      361 agaaaataat atttattttt cggtaaatgt gatcacaaaa gcattgccta ttccagaaag
      421 tcatgttatg acttttattt taacaaaaaa cacactcgtt actttgcgct atgccgatcc
      481 ccatccgata cgtactttta ttgaccagct agaaaaacgc tctatccata tacaaaatcc
      541 tctagatatt ttttatattt tagtggaagc ctgtgtcggt agaattgccg atatgtttga
      601 agtgtttgat gaaaaaacca accaacttgc tcagcattta agcagtatta taaaaagaac
      661 aaccaaaata aaacgcaatg aagcattgaa ccaaacattg ggcgaaatta atgatttaga
      721 aaatttatta tctaaatgtt ttcaaagttt atcgagtttg cagtttttaa tcggtttttt
      781 gaaacaagtt gcgcccaaaa ttaaattgaa aattgctaat ggttggccaa ttcttaatca
      841 agatttacag gcgatgatag atcatggtga atatttaacc gataaattaa gttttctttt
      901 agagtcgacc ttaggtctta ttaatatcga gcaaaccaat attataaaat catttacggt
      961 tttggctatg atatttatgc cacctacact gattgcaagt gtttatggta tgaattttcg
     1021 atacatgccc gagataaatt tagtgtgggg ttacccattt gctatggggg ttatgattat
     1081 ttctgctata ttacctaccg tattttttaa attgaaaggt tggatataat tacattttaa
     1141 aactctcttt cggattcgat ggcgtttcta gggttttacg ccagcgcttt gcatctatta
     1201 tgctatgttt gcgagatttt atggaagtgt tattcattat tttatcggta atagcaagaa
     1261 gaattgcaga aataacaaag gttaaatgaa taaccacttg ccaaagcata acctgcggct
     1321 caatattttc cgcattaata aaagttttga gtaaatgaat agaagatatt ccaatgagtg
     1381 ctgtggaaag ttttattttc agagtgcctg aattaacatg atcgagccat tcaggcaagt
     1441 cgggatgatg ttgaatacca attcttgtca caaacgtttc ataaccgcca acaataacca
     1501 taataattaa attggcaatc attactacat caattaatcc gagcacatga agcataacat
     1561 gggcttcatt gtgatgattt gcgtctttaa ttaataaata cagttcaatc ataaattgat
     1621 aaacgtaaac acactgggcg aaaataagac cgacataaag gggtgcttgc agccagcggc
     1681 taccaaaaat tattttactc aatccccggt attcattaag catactgatc cctaatagct
     1741 caaaaaaggc agtagagaga gaatattaac ataattaaaa atagaaacgc atggagatca
     1801 tgaacaaggc aaagtagtaa aaaccgttct aaacggtagc tgccaaactt aattaaagat
     1861 ttttacttga ttccattttt tgattatttt atattctcgg ggttatgctg gtgctctgta
     1921 atcttcttag caccaaactg ctaaagttgt ttacttatcc aaaaataatt taccacctag
     1981 taggtagggt gtaaaagtct atggttcgat ctaacaattt tttacgcaat gcctccatgt
     2041 ggctattagc ttccctattt tacgcctacc aatttgtttt acgggttttg cctaacatta
     2101 tgatgccgga tttaatgcgg caatttaata tcgatgctag catttttggg caattaagtg
     2161 gtgtttatta cataggatat gctggaatgc atattcctat aggcattttg ctcgataaat
     2221 ttggccctaa aaccatttta cctgtttgta tggttttggc agcttgcggc ttattacctt
     2281 ttgtttatgc gcaaacgtgg atttatcctt gcttgggacg cttcattata ggcgtaagtt
     2341 cttctgcagc tatacttggg ttgtttaaaa tcattcgaat ggcatatcga gaagaattgt
     2401 tcacccgtat gttgggtgca tcggtaacca taggtttgtt gggcgctata tacggtggct
     2461 accctgtacg ttggcttttg gaaaatatag gatggtacac cactttccat ctattaatat
     2521 tggtgggggt ggtcatggct gtcataacct ttatcatcgt ccccagtatt aaaaacccta
     2581 cttcaacttc cgtttggaca gacttaaaaa atttgttttt gaaccgtaaa attgtggccg
     2641 tttgttttct tgcagggtgc atggtcggac ccatggaggg atttgccgac ggttggagta
     2701 ctatttttct tacgcaaatt tatcacctgg gtaatgctat tgcagcgagc ttgccttcca
     2761 tgatttattt gggaatgtgc attggtgctc ctttattgag tttattggct gaaaaaacga
     2821 aagcgtatta tgaaattatc gcaatgtcag cttttatcat gtgtgcttct tttattctgc
     2881 tgttggcagg ttctctacag ttaagttttg tttctctggt attttttatc atagggctac
     2941 tctgtgctta tcaaattttg gccatttata aggcgtcttc ttacgcaaaa gaatccaatg
     3001 taggaatcac cacagcattt gccaacatga tcataatgtt ttttggctac gtatttcata
     3061 ccgcaattgg taaagtagca gtacattttt gggatggcac ggtactacaa ggtattccat
     3121 tttatagcac gtttgcgtta cgagcagcac ttggaataat tcctattatg ctcttcgtag
     3181 gtcttttggg ctttagcaca cttgctatac aagaaaaaag aacataatcg ataatttagt
     3241 gcgtttttaa ctagctattc tgctaaatct tgaaaaatca aggggttact gctatttttt
     3301 tattttaatt gcttaaaaat ttagcaccgt ggaactacgt aattttaata ataagcctag
     3361 aagagttgga tttgaaatcg aatatattgg tatccccatt cgagagtctg cgaaccttat
     3421 tcataagctc tatggtggta aaattgtaga aaaaaatgaa aatttaatta ttgtagaaac
     3481 accatggggt ccattccaac tcgaagttga cgtttactta ttagaaaaat tagcagccgc
     3541 atctaaaaaa aatatagaag aaaaaaaaat tgatgttcaa ggagctgcgc aatatatttt
     3601 atctaatctt ctgactgcat tcatgcccaa tgaaattgtg tgtcctccta tttttgcaca
     3661 ggacatagaa aaattagaac cgcttattgg aaatttgcga gaacacggag caaaaggcac
     3721 cagcgcttct tttgcttatg cttttggatt gcacatcaac gtggagcctt attctcttga
     3781 aacgtccatg ttgttgcagt atttacgtgc ttttgtttta ctttatgatt atttaaaaga
     3841 aacgatgcat atagatatca caagagcaat cactttattt gccaaaccct attctct
//
LOCUS       DASTIB010000262         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1421016, whole genome shotgun sequence.
ACCESSION   DASTIB010000262 DASTIB010000000
VERSION     DASTIB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421016"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VFP93_04530"
     CDS             complement(<1..122)
                     /locus_tag="VFP93_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET9843903.1"
                     /translation="MSKRAVEISVGLFILFGILSLIVLALRVSGLSDIYSGQEG"
     gene            complement(122..904)
                     /gene="mlaE"
                     /locus_tag="VFP93_04535"
     CDS             complement(122..904)
                     /gene="mlaE"
                     /locus_tag="VFP93_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HET9843904.1"
                     /translation="MRPIQYLGRNSLNFVQTTGKTGLFLFHALVGRPRPFVAFKLLIQ
                     QLYSVGHLSLLIIVVSALFIGMVLGLQGYTILVKFGADQALGQLISLSLVRELGPVVS
                     GLLFAGRAGSAITAEVGLMKATDQLSSMEMLGVDPLRRIIAPRFWAGQISMPLLALIF
                     SAVAIFGGYMVGVEWLGVDQGAFWGNMQAAVDFREDIVNGIIKSICFGTVVTWIAVYQ
                     GYECPPSSEGIGLSTTRTVVYSSLAILGMDFFLTAVMFGELR"
     gene            complement(901..1719)
                     /locus_tag="VFP93_04540"
     CDS             complement(901..1719)
                     /locus_tag="VFP93_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9843905.1"
                     /translation="MNSTTDSIIEIQNLGFKRGERSIFDKVNIQVARGQVTAIMGPSG
                     SGKTTLLRLIGGQLVPQEGTILVNGSNITKLKRDELYKVRKTMGMLFQSGALFSDLSV
                     YDNVAFPLREHTNLPETMIRDLVLMKLHAVGLRGARELMPAQLSGGMQRRAALARTIA
                     LDPNLLMYDEPFAGQDPISMGMLVQLIRLLNDALGLTSIIVSHDVMETCSIADYAYVI
                     AEGRVIGQGTPDELMKSQSSWLRQFMQGLPDGPVPFHYPAQNYATDLLEGREGL"
     gene            1898..>2841
                     /locus_tag="VFP93_04545"
     CDS             1898..>2841
                     /locus_tag="VFP93_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET9843906.1"
                     /translation="MDITKNKLLSRGKAVIHTEAEALFNLSKQLSSSFVSICEAILAS
                     RGRVVVLGMGKSGHIGAKIAATLASTGTPAFFVHPGEASHGDLGMITQDDIVLALSNS
                     GETLEILTLLPALKRFGISILAVTGNPKSTLAQNSSVHIHVPIEQEACTLGLAPTSST
                     TAMLALGDALAIALLESKGFSKENFALSHPGGSLGKRLLLTVESIMHKNAEIPAVKLG
                     ASLQDALIEMTRARLGTTAIVDENFCVKGIFTDGDLRRVFEKGVSIHVPIQDIMTTPC
                     YLIKKSTLAAEALRIMETRKINALLVVEDSGLLIGALN"
BASE COUNT          898 a          635 c          538 g          770 t
ORIGIN      
        1 tacccttctt gaccggaata gatatcactc aagccactca cacgcagagc taaaacgatg
       61 agacttaata taccaaatag gataaacaag cctacgctta tttcgacagc gcgtttactc
      121 atcagcgcaa ttctccaaac atgactgcgg ttagaaaaaa atccatgcca agaattgcta
      181 aggacgaata gacaacggtt cttgtagtgg ataaacctat cccctcagaa cttggtgggc
      241 attcataccc ttgataaaca gcaatccaag tgactacagt tccaaaacag atacttttta
      301 ttatgccatt gacaatatct tctcgaaaat caacagcagc ttgcatgtta ccccaaaaag
      361 caccttgatc cacgcctagc cattccaccc ccaccatata gcctccaaaa atagcaacgg
      421 cactaaaaat aagagcgagc aaaggcatag aaatttgtcc cgcccaaaaa cgtggcgcaa
      481 taatccgtcg caacgggtct actcccagca tttccatact ggatagttga tccgttgcct
      541 tcattaaacc aacctctgcc gtaattgcgg aacctgccct accagcaaat aacaagccac
      601 taacgacggg acctaactcg cgtaccaagc ttaaggaaat aagctgacct aaggcctgat
      661 cggcaccaaa ttttactaat attgtataac cttgaagccc aaggaccatt cctatgaata
      721 aggctgatac aacgatgatt aataaagaaa ggtggccaac agaatacaat tgttgtatta
      781 aaagcttaaa cgctacaaaa ggacggggtc gtccaaccag tgcatgaaac aaaaacaaac
      841 cggtcttgcc cgtagtttgt acaaaattta aagaattgcg acctaaatat tggatgggtc
      901 tcataaacct tctcttcctt ctaggagatc agtcgcatag ttttgcgcag gataatggaa
      961 aggaaccggg ccatccggta aaccttgcat aaattgtcgc aaccaacttg actgactttt
     1021 cataagttca tctggagtac cctgaccaat aactcgtcct tctgcaatga catacgcata
     1081 atccgcgata ctacatgtct ccataacatc gtgagaaaca atgatgcttg ttaaacctaa
     1141 tgcatcatta agtaaacgaa taagttgcac tagcataccc atagaaatgg gatcttgccc
     1201 agcaaaaggt tcatcgtaca taagaagatt tggatccaac gcaatagttc ttgctaatgc
     1261 agctctcctt tgcatgcctc ctgacaattg tgcaggcatc agttcccgtg caccacgtaa
     1321 tcccaccgca tggagcttca tgagaaccaa atcccgaatc atagtttcag gcaaattagt
     1381 atgttcgcgt aaagggaaag caacgttatc ataaacgcta agatcactaa ataaagcacc
     1441 gctttgaaat aacattccca tagttttgcg gactttataa agttcgtcgc gttttaattt
     1501 tgtaatatta gaaccattaa caaggatcgt tccttcttgt ggtactaatt gtccaccgat
     1561 caaacgcaaa agtgtggttt ttccactgcc actaggaccc attattgcag taacttgccc
     1621 ccgcgcgact tgaatattta ctttgtcaaa aatacttctt tcgccgcgct taaagcctaa
     1681 gttctgaatt tcaataatag agtcagtcgt tgaattcatt tttaccctat attcgaaact
     1741 taagctatca tacttttttc attgtgcatc agtaaaccta ttgatctcac cagctaccat
     1801 aaagtagtta actaacaaat ccgtagacca accaataatt gtggtaaaat aatcgaaaag
     1861 tccaggcttg tcattgatca aattttagga gcctttgatg gatataacaa aaaacaaatt
     1921 attatctcga ggcaaagcag taatacatac agaagccgaa gctttattta atttatccaa
     1981 acaactttct tcttcttttg tgtcgatctg tgaagcaata ttagcaagcc gcgggcgcgt
     2041 tgtagtatta ggcatgggaa agtctggaca tataggtgct aaaattgcag ctaccttagc
     2101 tagcacgggt acacctgctt tttttgtaca ccctggggaa gcgagtcatg gagacttagg
     2161 catgataaca caagacgata ttgtgttagc actgtctaat tcaggagaaa cgttggaaat
     2221 tttaacgctt cttcccgcat taaagcggtt tggtatttct attcttgcgg tcacgggaaa
     2281 tcctaaatct accttagccc aaaattcatc ggttcatatt cacgttccta tcgagcagga
     2341 agcttgtacg ttagggcttg caccgacttc gagcacaacc gctatgttgg cattaggcga
     2401 tgcattagcc atagcgcttt tagaaagtaa aggttttagt aaagaaaact tcgctttatc
     2461 gcacccaggt ggcagtttag gaaagcgctt attgttgacc gttgaaagta ttatgcataa
     2521 aaatgccgag attccggcgg ttaaacttgg cgctagttta caagatgcat taattgaaat
     2581 gaccagggca cgtttaggaa cgacagccat tgtcgatgaa aatttctgtg taaaaggaat
     2641 tttcaccgat ggtgatctaa gacgcgtgtt tgaaaaaggc gtatctatac atgtccccat
     2701 ccaagacatc atgactacac cttgctattt gattaaaaaa agtaccttag cagcagaagc
     2761 gttacgtatt atggaaacac gaaaaattaa tgcgttactc gtcgtggaag attccggttt
     2821 gttgattggt gctctaaaca t
//
LOCUS       DASTIB010000263         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_735662, whole genome shotgun sequence.
ACCESSION   DASTIB010000263 DASTIB010000000
VERSION     DASTIB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735662"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..905)
                     /locus_tag="VFP93_04550"
     CDS             complement(108..905)
                     /locus_tag="VFP93_04550"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9843907.1"
                     /translation="MRWILRPILLLCILALLAAFGVIAFKKQWDTSQSYTLSKPIKRG
                     NIIESVYGIGTVKANRKFELTSGITTTIKKLYVKEGDYINRGDKLVELQDATFIAPFS
                     GTITWLPFKEGEAIFAQSNILDLVDLNDRYLVVSLEQRGALKVRPQQKAKINFDGMRE
                     KTFEGLVQSVYSSGNDFLVRIQVKNLPPQVLPGMTGDVAIGIKEKQNVLLVPTVAIEN
                     NHVIIYGKNSSKPVPLTIGIVDAHMAEVVSGDISEHDILIIRDKLKP"
     gene            1109..2236
                     /locus_tag="VFP93_04555"
     CDS             1109..2236
                     /locus_tag="VFP93_04555"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET9843908.1"
                     /translation="MTGTYAYLESPGIHYKILQEKSHKIHIFTVDANEYQLDLVRAHD
                     KALGLETVFSMAARKGALLAINGGFFQVDGKFDGLPAGAIKINQQWFSLPQTPRSVLG
                     WNNSEWYFDRISTQTTCIIKNETLNVDGVNIPPRLKKNIFAYLPTFAPTTLTPSHFIE
                     IIVVDDKIQQIVKGGNAKIPPNGFVLSIRENAPLLHKFTVGSPITLKIKVKPLHSSPD
                     NWQRMDNIVNGIPLLIMDGKPLTDYEFESVDVENFINKSHARTAIGRTKTNQWLIVVA
                     EHQYDLSQSNRVLHHLKNLTSRNEKDQKNTHAIRGLTIPELREFMLQLGCHWAINLDG
                     GGSSTLVYNGKIMNNPYPELEEYFVKEWSRLVSDALILRKK"
BASE COUNT          742 a          452 c          393 g          706 t
ORIGIN      
        1 taaaaatcct gaaatcacca caaaagcaca cgcgcctaag aaaataccaa gtagcattag
       61 aaatgtttgc cagggttttg aaaacaaata ttttaaagca agtaggatca tggtttgagt
      121 ttatctctaa taatcaagat atcatgctcc gaaatatctc cagaaacaac ttcggccatg
      181 tgtgcatcaa caattccaat agtaagagga accggtttgg agctattttt cccataaatg
      241 attacatgat tgttttcaat agccactgtg ggaaccaaca aaacattttg tttttcttta
      301 atgccaatcg caacatctcc tgtcatacca ggcagaactt gtgggggaag attttttact
      361 tgaatacgta ctaagaaatc attgccactc gaatacaccg attgtactaa tccttcgaaa
      421 gttttttcac gcattccatc aaagtttatt tttgcctttt gttgaggtcg aacttttaaa
      481 gctcctcgtt gttccaagga aaccactaaa taacgatcgt ttaaatcaac gaggtctaag
      541 atgtttgatt gcgcaaaaat agcttcgcct tctttaaaag gaagccaagt gattgtgccg
      601 gaaaaaggtg cgataaaagt agcatcttgc aattctacta acttatcacc tcgattaatg
      661 taatcacctt ccttgacata taattttttt atcgttgtgg taataccgga agttaactcg
      721 aattttcgat ttgcttttac agtgcctatg ccataaacac tttctatgat attgccacgc
      781 ttgataggtt ttgaaagggt ataggattgc gacgtatccc actgtttttt aaaagcgata
      841 accccaaatg ctgctagaag tgccaaaatg cataatagga gtatagggcg aagaatccag
      901 cgcatgacta accttagtct aaattcagca agacgaactc tgtattttat aatagacttt
      961 ttttgctatt atctcgactg cacgatcttt gctaagaaaa ttagttgact aaaagaaggg
     1021 cgatctttta aaatttccca attctcttaa cacggtcaat gcatttatgt accgatgttt
     1081 catttttttt atgtgttttt ttttaaaagt gaccggaaca tatgcatacc ttgaatcacc
     1141 cggtatacat tataaaattc ttcaagaaaa atctcacaaa attcacattt ttactgttga
     1201 tgcaaacgaa tatcaactcg atttggttcg agcgcatgat aaagcattag gcctagaaac
     1261 cgttttctcc atggctgcac gcaaaggtgc acttttggct atcaatggtg gttttttcca
     1321 agttgatgga aaattcgatg gcttacccgc tggcgccata aaaatcaatc aacaatggtt
     1381 ttctttacca caaaccccac gttctgtcct tggctggaat aattccgaat ggtattttga
     1441 ccgaatcagc acccaaacca cctgtatcat aaaaaacgaa acattgaatg ttgatggcgt
     1501 taatatacca ccgcgactaa aaaagaatat tttcgcgtat ttaccaacat tcgctcctac
     1561 cacattaacc ccaagtcatt ttatagaaat tattgtagtt gacgataaaa ttcaacaaat
     1621 tgtgaaaggc ggtaatgcca aaatacctcc gaacggattt gttttatcca ttcgtgagaa
     1681 tgcgcctctt ctccataaat ttaccgtagg ctctccgatt acactgaaga taaaagtgaa
     1741 acctttgcat tcatcacccg ataattggca acgcatggac aatatagtca atggaatacc
     1801 cttattaatc atggacggta aacctcttac ggattacgaa tttgaaagtg tagacgtaga
     1861 aaattttatt aataaatcgc atgcacgaac cgctattggg agaacaaaaa ccaaccaatg
     1921 gttaattgtt gttgctgaac atcaatatga tctttctcaa tcaaatcgcg tactccatca
     1981 tttaaaaaat ttaacttcta gaaatgaaaa agaccaaaaa aacacgcatg cgatacgcgg
     2041 gttaactatc cccgaattaa gggaattcat gttacagttg ggatgtcatt gggccattaa
     2101 tttggatgga ggaggatctt caaccttagt ttataatggc aaaattatga acaatcctta
     2161 tcccgagctt gaagaatatt ttgttaaaga atggtctcga ctcgtttcag atgcattaat
     2221 tttgcgaaaa aagtaatacc ttgacctcaa ctttcgttct cacgcttcga ttttctaatc
     2281 gaaccgaaat taa
//
LOCUS       DASTIB010000264         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_795209, whole genome shotgun sequence.
ACCESSION   DASTIB010000264 DASTIB010000000
VERSION     DASTIB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795209"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFP93_04560"
     CDS             <1..207
                     /locus_tag="VFP93_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843909.1"
                     /translation="KINMPCIFNHFPNERGETINQVEYNKSVTRIYWKMRDRFWLNSC
                     SWSFSFLEKYLHRTYLIRMDQLLL"
     gene            233..1996
                     /locus_tag="VFP93_04565"
     CDS             233..1996
                     /locus_tag="VFP93_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9843910.1"
                     /translation="MKKIILFIKNIWPFLWSKEEGKRTLILCSFIATGIASLLTVASP
                     LLLKRIVDALLSPTQGYSSNAIFLTITYGLTWSLTQVLLHIRTVWVRKSCMLSVKNLI
                     YTLIKHLNRLPLSYHYDRKTGDVVSIIQRAEHGFSSFVQGVFWQIIPLTFEITLAIIC
                     ILKICGLAFGVGVFFILTAYLASLVVSVDKAALYQKQHTIQHAKASSHIVELLLNFEV
                     VRMFHSQDREENKLNNILNQKMQSEYLTIRKMETFGIIQTLILGTGLSILSIMAVFRI
                     QQGLLTPGDFILIVSYLLQFSLPVSYFGYVAREVRISLTNIKDFINILSIPTETEVHQ
                     IDTFPPSKEQVIQFKNVTFGYQKDRIILEDISFSLAKGKKLGIVGYSGEGKSTIAKLL
                     LRFFKVSKGDILINGYNINDIAIKSIRTKVGIVPQDIILFNDTLKNNLTYGHNNVSEQ
                     ELVNAIKLVRLDRLMNKLPDGLNTLVGERGIKLSGGEKQRIALVRTLLKNPELYIFDE
                     ATSSLDYATEKSILLDIKRISIGMTSIFISHRLSAVVDADEIIMIANGKIIERGNHNF
                     LLSQNGSYAALWNTQAAACEV"
     gene            complement(2231..2298)
                     /locus_tag="VFP93_04570"
     tRNA            complement(2231..2298)
                     /locus_tag="VFP93_04570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2271..2273),aa:Arg,seq:ccg)
BASE COUNT          727 a          392 c          435 g          750 t
ORIGIN      
        1 aaaatcaaca tgccatgcat tttcaaccac tttcccaatg aacgaggtga aacaatcaat
       61 caagttgaat ataataaaag tgttactcgc atttattgga agatgcgaga tcgtttttgg
      121 ttaaatagtt gttcatggtc attctctttt ctcgagaagt atttgcaccg cacctatttg
      181 atccgcatgg atcaacttct tctataaaga tgttcttgca acgttggaat atatgaaaaa
      241 aattatacta tttataaaaa atatctggcc atttctatgg tctaaagaag aaggaaaacg
      301 tacacttatt ttatgttcat ttatagccac cggaattgca agcctcttaa cggtggcttc
      361 tcctctttta ctaaaaagaa tagttgatgc tttattatct ccaacacagg gttatagtag
      421 taatgcaatt tttttaacca taacgtatgg cttgacctgg tcgctaacgc aggttttgct
      481 tcatattcgc acagtttggg ttagaaaatc ctgtatgctc agtgttaaaa atctgattta
      541 tacattaatt aaacacctta atcgattacc acttagttat cattatgatc gcaaaacagg
      601 agacgttgta agtattattc aacgggcaga acacggattt tcgtcatttg tgcaaggtgt
      661 attttggcaa attattcctc ttactttcga gatcacgctt gcaattattt gtatcctaaa
      721 aatctgtggg ctcgcttttg gagtgggtgt tttttttatt ttgaccgctt atcttgcctc
      781 acttgtagta agtgtcgaca aggcagcctt atatcaaaag caacatacaa ttcaacatgc
      841 aaaagcttct tctcatattg tcgaactatt attaaatttc gaagttgttc gcatgtttca
      901 tagtcaggat cgtgaggaaa acaagttaaa taatattcta aatcagaaaa tgcaaagtga
      961 atatttgact attagaaaaa tggaaacttt tggaattatt cagacattaa tattaggcac
     1021 gggtttatct attttatcta taatggctgt atttaggatt caacaagggt tgttgactcc
     1081 aggagatttt attttaatcg taagctactt attgcagttt tctttgccag taagctattt
     1141 tggatatgtt gcgagagagg taagaatttc attaacgaat attaaggatt ttattaacat
     1201 tcttagcata cctacagaaa ctgaagttca tcaaattgat acttttcctc cctcgaaaga
     1261 acaagtcatt caattcaaaa atgttacatt cggatatcaa aaagatagaa taattttaga
     1321 ggatatttct ttttcactgg caaaagggaa aaaattgggc attgttggct attcaggaga
     1381 aggcaaatcg acaattgcga aacttttatt aagatttttt aaggtgtcca aaggcgatat
     1441 tttaatcaat gggtataata ttaatgatat tgctattaaa tcaattcgaa caaaagtagg
     1501 aattgttccg caagatatta ttttatttaa tgacacgcta aaaaataacc ttacttatgg
     1561 ccacaacaat gtttcagaac aagaattggt taatgctata aaactagtac gccttgatag
     1621 acttatgaat aaactacccg atggtctcaa cacgttagtt ggtgaacgag gtattaaact
     1681 gtctggtggt gaaaagcagc gcatagcatt ggttcgtact ttgttgaaaa atccagaact
     1741 ttatattttt gatgaagcaa cttcatcttt agattacgcc accgaaaaga gtattttatt
     1801 agatattaaa agaatatcta taggtatgac ttcaatcttt atatcgcaca gactttctgc
     1861 tgtggttgat gccgatgaga ttattatgat agccaatggt aaaattatag aaagaggtaa
     1921 tcataacttt ttactatcac agaacggatc ttacgcagcc ctttggaata cccaagcggc
     1981 tgcgtgtgaa gtataaatga agtttctcgc tttcgcgggt gcgacggcat ccaggatcat
     2041 taagatagaa aactggattc ccgctttcgc gcgcaacacg atcatctaat tgcggcaccc
     2101 agcttttgtt cgagatgtta agacaagaaa aattgcattc gcggaagact aaggtgattg
     2161 atgaacttaa tcacgcgtaa caaagcttgg gaagtgaaat tctctgcgtg gaatgggacc
     2221 taccttttat tggcgcgccc tagaggattc gaacctctgg cctcagcctc cggagggctg
     2281 cgctctatcc actgagctac gggg
//
LOCUS       DASTIB010000265         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_228990, whole genome shotgun sequence.
ACCESSION   DASTIB010000265 DASTIB010000000
VERSION     DASTIB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228990"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VFP93_04575"
     CDS             complement(<1..1255)
                     /locus_tag="VFP93_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9843911.1"
                     /translation="MLIHLLITDFAVVKTLELDFSDGMTVITGETGAGKSILLNALSL
                     VMGERSDTHWVRPECDKAEIAATFDIAKLTGAILWLTDAELLNQNTTQECIIRRIIYK
                     NGRSKALINGKPVTSAQLRLLGENLLQIHGQHQHQLLLKPFEQLRLLDAFGQHQALLS
                     AVRKAYEVYEDLEQEIRHLRNVIADAHRISLLEYQLEELEQLEIASHECEDLCQEQRR
                     LSHAQSHLSSLTAAFEHLQHEDAGVMTLLQKALVHLQDLTMHYPKLQTVLDLLQTAQV
                     HVEESGIELNRLLNIIQLNPERLQEIEIRLSKMYAIARKHKVEPSQLRNFQNGLSEEL
                     TNIKESQARIGSLAEHLELTKQSYKKITHKLTKAREKAALHLAQEITHWFAPLGLKGA
                     SFRIDLEHDPENFSLTGLESVQYLVS"
     gene            complement(1262..2140)
                     /locus_tag="VFP93_04580"
     CDS             complement(1262..2140)
                     /locus_tag="VFP93_04580"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HET9843912.1"
                     /translation="MTMQFSRIGLMGKPNNPEVKSTFHKLIRSLRDFKLNLQIEEACA
                     PLASHLKVQTASAEDLAKESDLIIVVGGDGSLLNAARMVVNNNVPVIGVNRGRKGFLT
                     DITPQALTEELIPILNGEYFEEERFLLQANIYRDNHKVMEYIALNDVVLYSGHVARMI
                     EFEVNIDKSFVYRQRSDGLIISTPTGSTAYALSGGGPIIHPTLNAMVLVPMHPHTLSS
                     RPIVVDANSKLFIHLTKENELNPRLSWDGQVHADLNPNDAIVLEQYDKKLRLLHPKSY
                     DFYETLRGKLGWHIYQ"
     gene            2344..>3128
                     /gene="hrcA"
                     /locus_tag="VFP93_04585"
     CDS             2344..>3128
                     /gene="hrcA"
                     /locus_tag="VFP93_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET9843913.1"
                     /translation="MVIPSAIETVEENISDRAKSLLKHLVELYIQSGQPVGSKTLADS
                     AKISLSPATIRKVMNDLEHYGFVQSPHTSAGRVPTARGFRFFVDSLLTVHPLDQKQVR
                     QFQNQLNPNQTTNSLITTASSLLSEITQMIGIVTLPKQEKLILKYLEFIPLADNRTLV
                     VLVVNEKEVQNRIIVTEKPYSASELSQASNFLMQHFVGKDLSEARAELLQAIKREQLI
                     ANHLMETILEVSTKAFEKPKREEYVIAGEFNLLAQCDPPAMDH"
BASE COUNT          941 a          644 c          571 g          972 t
ORIGIN      
        1 tgcttaccaa atattgcacc gattcaagac cagtgagaga gaaattttcc ggatcatgct
       61 ctaagtcaat ccgaaaggaa gcacctttta aacccaatgg tgcaaaccag tgtgtaattt
      121 cttgtgctaa gtgcaaagcc gccttttctc gcgcctttgt caatttatgg gttatttttt
      181 tgtagctttg tttagtaagt tcaagatgtt cggcaagact acctatacgt gcttgcgatt
      241 cttttatgtt cgttaattct tccgataaac cattttggaa gtttctgagt tgcgagggct
      301 caactttatg ttttctagca atagcgtaca ttttagaaag gcggatttcg atttcttgta
      361 atcgctctgg attcagttga attatattca ataatcgatt taattctatc ccggattctt
      421 ccacatgcac ttgcgctgtt tgcaataaat ccaaaacggt ttgaagtttt ggatagtgca
      481 ttgttagatc ctgtaaatgt actaacgctt tttgcaaaag cgtcatcact cccgcatcct
      541 catgttgtaa gtgctcaaaa gcagccgtaa ggctacttaa atgcgattgc gcatgggata
      601 aacgtcgttg ctcttggcaa agatcttcac actcatgaga agcaatttcc agttgttcca
      661 attcttccaa ctgatattct aaaagactga tacggtgtgc atcggcgatt acatttctta
      721 aatgtcgtat ttcttgttca agatcttcgt aaacttcata ggccttacgc actgcactta
      781 gcagtgcttg atgttgacca aaagcatcta agagtcgaag ctgttcaaaa ggctttaata
      841 aaagttgatg ttggtgttgg ccatgtattt gcaataaatt ttcgcctaat aaacgcagtt
      901 gagcactcgt aacaggttta ccattaatga gcgctttgga acgtccattt ttatatatta
      961 tacgtcgaat gatacattct tgcgtggtat tttgatttaa aagctctgca tccgttaacc
     1021 ataaaatcgc acctgtcagt tttgctatat caaaagtcgc tgcaatttca gctttatcac
     1081 attcaggtct tacccaatgc gtatccgagc gctcgcccat aaccaaagac aatgcattta
     1141 ataatatact tttgccagca cccgtctccc cggtaataac agtcatgccg tctgagaaat
     1201 caagctccaa agtttttact actgcaaaat cagtgattaa taaatggatt aacataaagc
     1261 ctcattggta tatgtgccat cccaatttac cccgtaaagt ttcataaaaa tcgtaacttt
     1321 ttgggtgtag taatcgtaat tttttgtcat attgttctag aacaattgca tcgtttgggt
     1381 ttaaatctgc atgtacctgg ccatcccagc ttaatcgcgg gttcagttca ttttccttcg
     1441 tgaggtgaat gaaaagttta gaatttgcat caaccacaat aggacgcgaa cttaaggtat
     1501 ggggatgcat gggcactaaa accatcgcat tcaaagttgg atggataata gggcctcccc
     1561 ccgataaagc atacgcagta gaacctgttg gcgttgaaat aattaaacca tcggatcgtt
     1621 gtctataaac aaaggattta tcgatattaa cttcaaattc aatcattctc gctacatgac
     1681 ccgaataaag tacgacatca tttaatgcta tgtattccat taccttatgg ttatcacggt
     1741 aaatgttggc ctgtagtaag aagcgttctt cttcaaaata ctccccattt aaaataggaa
     1801 tcaattcttc cgttagcgct tgcggggtta tatccgttaa aaatcctttt ctcccacggt
     1861 taaccccaat cacaggtacg ttattgttaa ccaccatgcg ggcagcattc aaaagactgc
     1921 cgtcgccacc caccacgata ataagatcac tttctttagc caaatcttca gcggaggccg
     1981 tttgcacctt taaatgtgaa gcaaggggcg cacaggcttc ttcaatttgg agatttagtt
     2041 taaaatctct taaagaacga atcagtttat gaaaggtact tttaacttca ggattgttag
     2101 gcttacccat gagcccaatt cttgaaaatt gcatggtcat ttttttttcc tcacgaggga
     2161 cctcaccata caatgaacca aaactactga caagatagcg tttatcctga ttcaactaag
     2221 ttaaacaaca aaggtatttc agaatttttt tttaaatcta gctttcaagc ctagactcct
     2281 tttactccct attaacgcag tttagaataa acaacgttag aacattactt tactcaataa
     2341 actatggtaa tcccaagcgc tatcgaaaca gtagaagaaa atataagtga cagggcaaaa
     2401 tccttattaa agcacttggt agaattatat attcagtcgg gtcagccggt gggatcaaaa
     2461 acattggctg acagtgcaaa aatttcttta agtcctgcaa caatccgtaa agtgatgaat
     2521 gatctggaac attatggttt tgtgcaatct ccacatactt ccgccggtag ggtacctact
     2581 gcgcgtggtt ttcgtttttt tgtggatagc ttattaacgg tgcatccact tgatcaaaaa
     2641 caggtacgac aatttcaaaa ccaactaaat cccaaccaaa ccaccaatag tttaattacc
     2701 accgcctcca gtcttctatc tgaaataact caaatgattg gtatagttac cttaccaaaa
     2761 caagaaaaat taattctaaa atatttagaa ttcatcccat tggcagacaa tagaacgctt
     2821 gttgtgttag tcgtaaacga gaaggaagtg caaaatcgca ttattgttac agaaaaacca
     2881 tattcagctt ccgagctttc tcaagctagt aattttttaa tgcaacattt tgtggggaag
     2941 gatttaagcg aagcacgcgc tgaattattg caagctatta aacgtgaaca attaatcgca
     3001 aaccatctga tggagacaat tttagaagtt tcaaccaaag cttttgaaaa gcccaagcgc
     3061 gaggaatatg tgatagcggg tgaatttaat ttgcttgctc aatgtgaccc cccagccatg
     3121 gaccactt
//
LOCUS       DASTIB010000266         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_880830, whole genome shotgun sequence.
ACCESSION   DASTIB010000266 DASTIB010000000
VERSION     DASTIB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880830"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1656
                     /gene="ctaD"
                     /locus_tag="VFP93_04590"
     CDS             13..1656
                     /gene="ctaD"
                     /locus_tag="VFP93_04590"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET9843914.1"
                     /translation="MSNSSPISADISHEDEHHDHGPAQGITRWLFTTNHKDIGTLYLW
                     FSFLMFLSGGALAMLIRAELFQPGQHLLNPELFNQMTTMHGLIMVFGAVMPAFVGLAN
                     WQIPLMIGAPDMALPRMNNWSFWILPVAFAIMGSTLFMEGSAPNFGWTFYAPLSTTYG
                     PPSTDFFIFAIHLMGMSSIMGAINVIATILNLRAPGMTMMKMPIFCWSWLITAFLLIA
                     VMPVLAGVVTMVLTDRNFGTSFFNAGGGGDPVLFQHVFWFFGHPEVYIMILPAFGIIS
                     QIVPTFARKPLFGYASMIYAISSIAFLSFIVWAHHMFTVGMPLAGEIFFMYATMLIAV
                     PTGVKVFNWIATMFKGAMSFETPMLFALAFIILFTMGGFSGLMLAITPADFQYQDTYF
                     VVAHFHYVLVPGAIFSIIAAVYYWLPKWTGNYYSETLGKLHFWLSAISVNVTFFPMHF
                     LGLAGMPRRIPDYALQFTDFNQIVSIGAFVFGASQVLFLYIVIKTILGGIRASDKVWD
                     EPKGLEWTLPSPPPYHTFSTPPHLSFLEIPGETKIFPKN"
     gene            1660..2181
                     /locus_tag="VFP93_04595"
     CDS             1660..2181
                     /locus_tag="VFP93_04595"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET9843915.1"
                     /translation="MVQNTIRKHLLIMTIIVIGMFAFCFALVPLYNVLCTLTGLNGKT
                     SPNEVLMPQVNIDTSRYVNVELLATRNGNLSANFYPQTKKFRVHPGEFIDTAFWVENL
                     ESKPLVLQAIPSVAPGLAAEHIKKQVCFCFQHQELEGNQRMKMPLRFMVDSKLPKKYT
                     TITLAYTLFDVTS"
     gene            2193..3068
                     /locus_tag="VFP93_04600"
     CDS             2193..3068
                     /locus_tag="VFP93_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9843916.1"
                     /translation="MAQKYEHYYVPSQSHWPIVGSIGLGAIALGIANLLHEKGIGHYL
                     FFVGSLLIVWMMYGWFRDVIKESHKGLYSAQMDRSFRWGMAWFIFSEVMFFGAFFGAL
                     FYARLFSVPWLGGAIGGESTHQLLWENFQATWPLLKTPSPEGFTQPLKAMEAWGLPAL
                     NTLILLTSGVTITWSHWGLLKNKRNQLILGLFMTIALGCIFLGLQALEYHEAYHEYHL
                     RLDSGIYGTTFFMLTGFHGAHVTLGTIMLITIFLRSVRGHFKPESHFAFEAVAWYWHF
                     VDVVWVILFIFVYWL"
BASE COUNT          795 a          586 c          657 g         1068 t
ORIGIN      
        1 gaggattgtt ggatgagcaa ctctagccct atttctgcgg atattagtca tgaagatgaa
       61 catcacgatc acggacctgc tcagggtatt acacgttggc tgttcacaac taatcataaa
      121 gatataggca cactgtattt atggtttagc tttttgatgt ttttaagcgg tggcgcactg
      181 gccatgttaa ttcgagcaga attatttcaa cccggtcaac atttgttgaa cccggaattg
      241 tttaatcaaa tgactaccat gcatggtttg attatggtat ttggggcagt aatgccagca
      301 tttgtgggac ttgctaattg gcaaattcct ttgatgattg gcgctcccga tatggctcta
      361 cctagaatga ataattggag tttttggata ttacctgtag cttttgccat tatgggtagc
      421 actcttttta tggaaggatc ggcgcctaat ttcggatgga ctttctatgc gccattgtct
      481 acaacgtatg gaccacctag caccgacttt tttatatttg cgatacattt aatgggtatg
      541 tcttcgatta tgggggcaat taacgttata gctaccatat taaatctacg cgcaccaggc
      601 atgaccatga tgaaaatgcc cattttttgt tggtcttggc taatcaccgc atttttactt
      661 attgcggtaa tgcctgtatt agctggagtg gtgaccatgg ttttaacgga tcgcaacttt
      721 ggtacaagtt tttttaatgc gggaggtggt ggtgatcccg tgctgtttca gcacgtattt
      781 tggtttttcg gccatcctga agtttatatt atgattttgc ctgcttttgg gattatttcg
      841 caaattgttc ctacttttgc gcgtaaacct ttatttggtt atgcttccat gatttatgca
      901 ataagctcta ttgcatttct ttcttttatt gtttgggccc accatatgtt tacggttggc
      961 atgccattag caggcgaaat attctttatg tatgccacta tgttgattgc agtgcctacc
     1021 ggagttaagg tctttaactg gatcgcgacc atgtttaaag gtgcgatgag ctttgaaaca
     1081 cccatgcttt ttgcgcttgc ttttattatt ttatttacga tgggtggttt ttccggatta
     1141 atgttggcga ttacacccgc agattttcaa taccaagata cgtattttgt ggtagcacat
     1201 tttcattatg tgttagtgcc cggagctatt ttttccataa tcgcagcagt atattattgg
     1261 ctccctaaat ggacgggtaa ttattattcc gaaacacttg ggaaacttca cttttggtta
     1321 tcggcgattt ctgtcaatgt gacttttttt cctatgcatt ttttaggttt agccggtatg
     1381 ccacgccgaa ttcctgatta tgcgctgcaa tttaccgatt ttaatcaaat tgttagcata
     1441 ggcgcttttg tgtttggcgc ttctcaagtt ttatttttat acatcgtgat aaaaaccata
     1501 ctcggcggca ttcgagcttc ggataaagtt tgggatgaac ccaaaggttt ggaatggaca
     1561 ctaccatcac ctccccctta tcatactttt agcacaccac cacatctttc ttttcttgaa
     1621 ataccaggag aaaccaaaat ttttcccaaa aattagccca tggtacaaaa tactatacgt
     1681 aagcatctgc ttattatgac gattatcgtg ataggaatgt tcgctttttg ctttgcattg
     1741 gtgccccttt ataatgtttt atgcacgcta actggattga acggtaagac gtctccaaat
     1801 gaagtgctta tgccacaagt aaatattgac acctcaaggt atgttaatgt ggagcttttg
     1861 gcaactcgga atggaaattt gtcagcaaat ttttatccgc aaaccaaaaa atttcgggta
     1921 catccgggtg aatttattga tacagctttt tgggtagaaa accttgagtc gaagccttta
     1981 gttttacaag ctataccaag tgttgctcct ggtttagccg cggaacacat taaaaaacaa
     2041 gtgtgttttt gtttccaaca tcaagaactg gaagggaacc aacgtatgaa aatgcctctt
     2101 cgttttatgg tcgattcaaa acttcccaaa aaatacacta cgataacttt agcgtacact
     2161 ttatttgatg tgacctcata ggaagcaaaa aaatggcaca gaaatacgag cactattatg
     2221 taccttccca aagccactgg cccattgttg gctccatcgg attaggagcg atagcacttg
     2281 gcattgcgaa tcttttacac gaaaaaggta ttggtcatta tttatttttt gtgggttcct
     2341 tgctgattgt ctggatgatg tatggttggt ttcgagatgt tataaaagaa agccataaag
     2401 gattatatag cgcacaaatg gatcgctcgt tccgatgggg aatggcgtgg ttcatattta
     2461 gcgaagttat gttttttgga gctttttttg gcgcactttt ttatgcgcga ttattttctg
     2521 taccatggct tggcggtgct attggaggtg aatctaccca tcagcttctt tgggaaaatt
     2581 ttcaagcaac ttggcctcta ttaaaaacac ctagccccga gggctttacc caacctttaa
     2641 aagcgatgga agcttggggg ttaccggcac ttaatacttt gatcctctta acgagtggtg
     2701 taaccattac ctggtcacat tgggggttat taaaaaataa acgcaaccaa ttaatcttag
     2761 gtttatttat gactattgcg cttggttgta tttttttagg gctacaagcg ctcgaatacc
     2821 atgaagctta tcacgaatac catttacgat tagattcggg gatctacggc accacttttt
     2881 ttatgctgac cggttttcat ggcgcacatg taaccttagg tacgattatg cttattacaa
     2941 tcttcttacg aagtgttaga gggcacttta aaccggaaag ccattttgct tttgaagcag
     3001 tggcatggta ttggcacttt gtagacgtag tatgggtgat attatttata tttgtttact
     3061 ggctgtaatt actttaaacc atggggtgtt atccatccca tgaaaa
//
LOCUS       DASTIB010000267         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_155176, whole genome shotgun sequence.
ACCESSION   DASTIB010000267 DASTIB010000000
VERSION     DASTIB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155176"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFP93_04605"
     CDS             complement(<1..234)
                     /locus_tag="VFP93_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843917.1"
                     /translation="MPKEPIIPPYDPYDPRQNELLTIQPSLKQPVMQLEQVQAYLHNV
                     EEVLTHSKEYIRIRGAMNTLEYITSYNYYCLNPL"
     gene            451..2208
                     /gene="argS"
                     /locus_tag="VFP93_04610"
     CDS             451..2208
                     /gene="argS"
                     /locus_tag="VFP93_04610"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9843918.1"
                     /translation="MKSLKTALLELFTHTLHQVFPELQEQSIEITVSANEKFGAYQCN
                     SSMRFAKIVQMPARAIAEKWMDALQQSEHPYKKWIDKLEVAGPGFINIWLNPITLASF
                     CDAMFIHPQFGIETDTTQKVIVDFSSPNIAKEMHVGHLRSTIIGDSLCRLFEFFGLNV
                     LRLNHVGDWGTAFGMLIAYLKEEIKENIYDQDVQLSDLVQWYKASKARFDSDADFKKR
                     AQQEVVSLQSGDPENLKLWQLICNISERAYHAVYEVLGVTIQDRGESFYNPMLQTIVG
                     LLEQQALIEVSEGAKCVFLPGFKNREGEPLPFIIQKSDGGFNYATTDLAALYHRVHEE
                     KGDRLIYVTDAGQATHFAMVFEVAARAHFYEPQQVRCDHVPFGVVLGPDGKKFKTRSG
                     ETERLIDLLYTAITKAKELIQQRQMTQNNFLNDEEVEHLSYVLGIGAVKYADLSCNRI
                     NDYVFSYDRMLKFEGNTAAFMLYSYVRVHGIKRKIGDVTIDLQEIPLALQEPQEKLLG
                     QHLARFPDTLLGMAQDLMPHRLCEYLYDLAVHFNAFFRDCRVEGDSNQMPRLKLCELT
                     ARVLRCGLSILGLETVDKM"
     gene            complement(2211..2549)
                     /locus_tag="VFP93_04615"
     CDS             complement(2211..2549)
                     /locus_tag="VFP93_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843919.1"
                     /translation="MSSLQDPFILLCERYLLSKEQSERILLYVVTMIEDLNKKTALEG
                     AEKNLLVQDCIHDFMQKIERALELNEAEMADLKGSGLQPQVIAQKRDQNIHAILDTFI
                     PTQNKKTHAP"
     gene            complement(2635..>3169)
                     /locus_tag="VFP93_04620"
     CDS             complement(2635..>3169)
                     /locus_tag="VFP93_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9843920.1"
                     /translation="SYLINLSRGDVVDLVALEQCLQTKHLAGAALDVYPEEPQESSAP
                     FSLSLQNHPNVILTPHIGGSTQEAQENIGHFVLNRLHEYLELGATIGCLNLPQLQLPE
                     IQGSHRIIHIHRNVPGILAQINSILAKHDVNVVGQYLKTEEELGYVITDTNVLNGESF
                     LKELEHIEHTLRLRILY"
BASE COUNT          954 a          571 c          663 g          981 t
ORIGIN      
        1 taacgggttc agacaatagt aattatagga agttatatat tccaaggtat tcatagctcc
       61 acgaatgcga atatactctt tgctatgggt aagaacttct tcaacgttat gcaaataggc
      121 ttggacctgc tctaactgca tgacgggttg tttcaaggaa ggttggatgg ttagtaattc
      181 attttgcctt ggatcataag gatcatacgg aggaatgata ggttctttgg gcatataaca
      241 accgttctaa ttacatgttt ttagtttaac aagaaaaatt tagaatacca accaagttca
      301 agtaaatata agtttgaact gtaaaatcta atttcggcaa tataactata aatatatttt
      361 atttcaaata cttggaatgc attgtttgaa gcgtgtgcat gaagtacaat caaacaatta
      421 tacatataag tttaataact aataaaatta atgaaatccc tcaaaactgc acttcttgaa
      481 ttattcacgc acacgttgca tcaagtattt ccggaacttc aagaacaatc catagaaatt
      541 accgtcagtg ctaacgaaaa atttggtgct tatcaatgca atagtagtat gcgttttgca
      601 aaaattgtgc aaatgcctgc acgggctatc gcggagaaat ggatggatgc cttacaacaa
      661 tccgagcatc cctataaaaa gtggatcgat aagctcgaag ttgccggacc aggttttatt
      721 aatatctggc ttaatccgat tacgcttgca agcttttgcg atgctatgtt tatacatcca
      781 cagtttggga tcgaaaccga tacaacccaa aaagttattg tcgatttttc atcgcctaat
      841 attgctaaag aaatgcacgt ggggcatttg cgatccacca ttatcgggga ctcactgtgt
      901 cgtctttttg aattttttgg tttaaacgtg ttgcgcttaa accatgtggg cgattgggga
      961 acagcgtttg gtatgctcat tgcgtatctc aaggaagaaa ttaaagaaaa tatatacgat
     1021 caagacgtac aattaagtga tttagtgcaa tggtataaag catcgaaagc gcgttttgat
     1081 agtgatgcgg attttaaaaa gcgtgcgcaa caagaagtgg tatcattaca aagcggggat
     1141 ccggaaaatt taaagctatg gcagcttatt tgtaatataa gtgagcgcgc gtatcatgcg
     1201 gtttatgagg tgttaggcgt aacaatacaa gatcgcggtg agtcttttta taatcccatg
     1261 ttgcaaacta tagttgggtt attagagcag caagcattaa ttgaggtgtc ggagggtgca
     1321 aagtgtgttt ttttacctgg gtttaaaaac cgagaaggag agcctttgcc atttatcatc
     1381 caaaaatccg atggtggttt taattacgca acgacagatt tagcagcgct ttaccatcgg
     1441 gtccacgaag aaaaaggcga tcgactcatt tatgtcaccg atgcgggaca agctacacat
     1501 tttgctatgg tatttgaagt agcggcaaga gcacattttt atgaaccaca gcaagtacgt
     1561 tgtgatcatg tgcccttcgg cgtggtgctc ggaccggatg gcaaaaaatt taaaacacga
     1621 tcgggagaaa cggagcgtct tatcgattta ttatatacag cgatcaccaa agctaaagag
     1681 cttattcaac aacgtcaaat gacgcaaaat aatttcttaa acgacgagga agtggaacat
     1741 ctttcttacg tattagggat aggtgccgtc aaatatgccg atttatcctg taaccgcata
     1801 aacgattacg tttttagtta tgatcgcatg ttaaaatttg aggggaatac cgcagcattc
     1861 atgctgtatt cctacgtacg tgtgcatggc attaaacgca aaattgggga tgtaactatc
     1921 gatcttcaag aaatccctct agcgcttcag gagcctcaag aaaaattact tggtcagcat
     1981 cttgctagat tcccggatac tttactgggg atggcgcagg atttaatgcc gcatcgattg
     2041 tgcgaatatc tttatgattt agccgtgcat ttcaatgctt tttttcgaga ttgtcgtgtc
     2101 gaaggcgatt caaaccaaat gccgagatta aagctatgtg aattaacggc acgtgtacta
     2161 cgttgcggcc tatccatttt aggtttagaa acggtggata aaatgtaaat ctaaggggcg
     2221 tgtgtttttt tgttttgcgt tggaataaac gtatcgagaa tagcatgaat attttgatcg
     2281 cgcttttgcg caataacttg aggttgtaga cccgatcctt ttagatcggc catttctgcc
     2341 tcattgagtt ctaatgccct ttcaattttt tgcataaagt catgaatgca atcctgcact
     2401 aataaatttt tttcggctcc ttctaaagcc gtttttttat taagatcttc tatcatcgtg
     2461 accacataaa gcaaaatacg ttcggattgt tctttactta aaaggtaacg ttcacaaagc
     2521 aagataaatg gatcttgaag actagacata cgatcctttg ggttagttac acgcttgcag
     2581 ctattagctt atacttataa agtagcatac cttttttatt cacctaggaa ctgcttaata
     2641 tagaatgcgc aaacgtaggg tatgttcaat atgttccaat tctttcagga atgattcccc
     2701 gtttaacaca ttggtatcgg taataacata accaagctct tcttctgttt ttaaatattg
     2761 accaacaacg tttacgtcat gttttgctaa aatgctatta atttgtgcca ggattccagg
     2821 cacattgcga tgtatgtgta ttatacgatg tgagccttgt atttcgggca attgcagctg
     2881 tggtaaattt agacagccga tggttgcgcc aagctccaaa tattcgtgta aacggttcaa
     2941 cacaaagtga ccgatatttt cttgtgcttc ttgggtactg cccccaatat ggggtgttaa
     3001 aataacattg gggtgatttt gtaaagacaa agaaaatgga gctgaacttt cttgtggctc
     3061 ttctggataa acatcaagag cggcacccgc taaatgttta gtttgcaaac actgttctaa
     3121 tgcaactaga tcgaccacat cccctcgact taaatttatc aggtaactt
//
LOCUS       DASTIB010000268         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_868764, whole genome shotgun sequence.
ACCESSION   DASTIB010000268 DASTIB010000000
VERSION     DASTIB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868764"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..628
                     /locus_tag="VFP93_04625"
     CDS             23..628
                     /locus_tag="VFP93_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9843921.1"
                     /translation="MNSLAENLLNIHITPQAQAHFVELILKEDEPEMNLRIFLDRPGL
                     PMAEVGITFCLPGEHKATDLVMDFDNFKLYVDKISAPFLQNAKIDYQTDNLGGQLAVT
                     APNLRGSKPSDSASLSEKIEYVLNSEINPNLAGHGGMVSLVEISNNNDVILRFGGGCH
                     GCGMADVTLKQGIQKTLKEQFPEINQVIDATDHSLGENPYY"
     gene            640..831
                     /locus_tag="VFP93_04630"
     CDS             640..831
                     /locus_tag="VFP93_04630"
                     /inference="COORDINATES: protein motif:HMM:NF041950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBU_0585 family protein"
                     /protein_id="HET9843922.1"
                     /translation="MFWKSKKFGFRSDIDSFFHEFDKNRTQLPASRKAEIAKHQKIFY
                     KRDKPVEEHKDLLWQEFSN"
     gene            943..>2181
                     /locus_tag="VFP93_04635"
     CDS             943..>2181
                     /locus_tag="VFP93_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843923.1"
                     /translation="MEPSVKQVLAYWRWFAACELLNDFNVSSHQIMVANFDLVTGKCM
                     DQELQKQAPCEFWLCVFQIQHSGKIIPCVWLPVCLDPSGNLAPSNAFALPYCPSYLFD
                     SFAPLGGLFGKIEFFHNVLKDLFFENGSLNTQLTWQSYYQKTCDLIQYLHANQPNVQR
                     MHVLSDQHACFFSKNLTWLNEENYENSSPLAKSILEIDESPSFPFMDVPQLVQDAHLL
                     CGTIEQKSLTSTQMDIAAHALHFQNIQAVRTPVGSHFEDLLQVLIASIFIQKNLKNQE
                     FSVAILSDSLSENNFEALYIDRKPSSAFIDETATLLDRPVASEMEAFKLLKNYLEECV
                     KSYKKNILAASFAFTGDTQKLNTMRGQRETLQKIDEEHENLLDKLNEITQKWKEYSKP
                     SFFQNIKFFFLRVAKKERIST"
BASE COUNT          727 a          366 c          402 g          686 t
ORIGIN      
        1 ttccccaata aggcaatatt acatgaatag cttagcagag aaccttttga atatacatat
       61 cacaccacaa gcacaggcgc attttgttga attgatttta aaagaagatg agccagagat
      121 gaatcttcga atttttttag atagaccggg cttacctatg gctgaagttg gtataacatt
      181 ttgtttaccc ggtgaacata aagccacgga tttagtcatg gattttgata attttaaact
      241 ttatgtagat aaaataagtg cccctttttt acaaaatgca aaaattgatt accaaacgga
      301 caatttaggt ggacaattag ctgtgactgc gccaaattta cgcggttcta aaccttcgga
      361 ttctgcatct ctttcggaaa aaatagaata tgtgttaaat agtgaaatta atcctaattt
      421 agcaggacac ggaggtatgg taagcttagt agagatctcc aataataacg atgtaatctt
      481 acgctttggt ggtggttgtc atggatgtgg tatggccgat gttacgttaa aacaaggcat
      541 acaaaaaacg ctaaaagaac aattcccaga aattaatcaa gttatcgatg caaccgatca
      601 cagtcttggt gaaaatccct attattaaca aggacaacta tgttttggaa atcaaaaaaa
      661 ttcggatttc gaagtgatat tgatagtttt tttcatgaat tcgataaaaa tagaacgcaa
      721 ttgcctgcct cacgcaaagc ggaaatagca aagcaccaaa aaattttcta taaacgcgat
      781 aaacctgtag aagaacataa agatctttta tggcaggaat tttcgaatta attttatgga
      841 ttgtttaaac ttttattatc agtgtgccct cttttagctt gttggttggg tcatttattt
      901 tatgtaacat cgtataaata ataatgcaag aaaggaattc agatggaacc aagtgttaaa
      961 caagtactag cttactggcg atggtttgct gcttgcgaat tattaaatga ctttaatgtt
     1021 tcttctcacc aaataatggt tgccaatttc gatcttgtaa cagggaaatg tatggatcag
     1081 gaattacaaa aacaagcacc ctgtgaattt tggctatgcg tttttcaaat tcagcattcg
     1141 ggcaaaatta taccctgtgt ttggttgccc gtgtgtttag atccttcagg caatcttgct
     1201 ccttcgaatg cgtttgcttt accgtactgc ccatcgtatt tgttcgactc cttcgcaccg
     1261 cttggtggat tgtttggtaa aattgaattt ttccataatg tgctcaaaga tttatttttt
     1321 gaaaatggat cattgaatac gcaactgact tggcagtcct actatcaaaa aacgtgtgat
     1381 ttaattcaat acctgcatgc aaatcagcca aatgtacaga gaatgcatgt attgtccgat
     1441 cagcatgcat gttttttttc taaaaattta acgtggttaa acgaagaaaa ttatgaaaat
     1501 agttcaccgt tagcaaaaag cattctggaa atcgatgaat cgccaagctt tccttttatg
     1561 gatgtaccgc agcttgtcca ggatgcgcac ttgctatgtg gtacgataga acaaaagagt
     1621 ctaacatcta cacaaatgga tatagcggca catgcattac actttcaaaa tattcaagcg
     1681 gttcgcactc ccgtcggaag tcattttgaa gatcttttac aagtattaat tgccagtata
     1741 tttattcaaa aaaatctaaa aaatcaagaa ttttctgtcg ctattttaag tgattcctta
     1801 tccgaaaata attttgaagc gttatacatc gatcgtaaac cttcgtcagc ttttatagat
     1861 gaaacggcaa cattactcga tcgtcccgtt gcgagtgaaa tggaagcttt taaattgtta
     1921 aaaaattact tggaggagtg cgttaaatct tataaaaaaa atattctcgc ggcaagcttt
     1981 gcttttacag gggatacgca aaaattaaat accatgcgtg gacaacgtga aacattgcag
     2041 aaaattgatg aagaacacga aaatttactc gacaaattaa atgaaattac gcaaaagtgg
     2101 aaagaatatt caaaaccttc tttttttcag aatataaaat ttttcttttt aagggtcgct
     2161 aaaaaagagc gaatttcgac a
//
LOCUS       DASTIB010000269         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_274213, whole genome shotgun sequence.
ACCESSION   DASTIB010000269 DASTIB010000000
VERSION     DASTIB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274213"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFP93_04640"
     CDS             <1..639
                     /locus_tag="VFP93_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9843924.1"
                     /translation="QQGAPLEYVRALFDQYAFNYDTHVKTMLNYQVPEKMRQAIAPFA
                     TKATKAWDVLDLGCGTGLCAPYFTDVAGKMVGVDISPNMIEVANQKGGYHKLHVEDAL
                     NFLAKTQYTYDLIIAADVFVYFAELHAILSAIGLKLVPQGFCIFSIEKCEDSEGPFIL
                     RKTGRYAHSASYLQTLCAQLHLLVLFQEETILRKQEDQDVYGYIIIVQKISV"
     gene            700..1293
                     /locus_tag="VFP93_04645"
     CDS             700..1293
                     /locus_tag="VFP93_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9843925.1"
                     /translation="MEWLQELHFLHYILIGFGLVVLFYFMVVYNSLVALKNNVKKAWA
                     NIDVLLKQRHEELPKLVTTCKQYMGYERDVLEKITELRNTVNQMRTSGDILGLGSAES
                     HLRDALGRLFALSENYPDLKADSTFLHLQARISSLENAIADRRELYNENATLNNIRIE
                     QFPDIVVAFIFRFKHQPMLEFQVEELGPVDMNALFKD"
     gene            1296..2240
                     /locus_tag="VFP93_04650"
     CDS             1296..2240
                     /locus_tag="VFP93_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843926.1"
                     /translation="MHSHYQSNYDLDLTLLCEIPEMWSFGVLSIFVVFCIGFFYAFYK
                     NLLRAHAVRDTPTSKIRSAHQGYVELMGTVGKSPEGTLQGVLSKLPCAWYQYKIEHYH
                     DKKWTVLEQGYSQNILVLEDTTGLCYVDPLKAHITTHSYSRWQGTKRYPNRMPQGFWQ
                     NLFWKSGPYRYTEWRMEEGMPFYALGHFVTFSKAAGLEVNAQNVQQLLKKWEKKHKTL
                     LAQLLAQHSHATANQTEKTLSYQAKKEMLRDAYEKAHSTFNVLSGYGLDRRQPFIIST
                     KEVGRLSSGYFWDAMLWALAFIFLFGLTASVIGFRISC"
     gene            complement(2224..2607)
                     /locus_tag="VFP93_04655"
     CDS             complement(2224..2607)
                     /locus_tag="VFP93_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843927.1"
                     /translation="MGLKGIHFDGDELLDQIITEIYAYLHGTGKYRFDRWFGSRLHHI
                     QWVSWAEVTHELYHFIKDPPLQYSNFIHCLETQKKRFPPSNGYSRKLEELAHFLAKGP
                     SFSEEYQRNFKPIPMIKRKKFNKKF"
     gene            2937..3764
                     /locus_tag="VFP93_04660"
     CDS             2937..3764
                     /locus_tag="VFP93_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843928.1"
                     /translation="MSHLATKLKLFIVLSLCSQVSHALIIHSDPSHSLENLGDFQAEV
                     HWDGLGLNIQLTNLSEPTNGGYITGFLMNLPGDTTFQYATTDSKFSPLMPEKGPYYGT
                     KPYGYFDYGFTLGGSFLGGGKPHKGGIGVGQSANFTLAGWLNFPDDLDINNADVVENY
                     FFDALKSPELGSISFLTRFRGFEDEGSDKVPGVAQLHDLNFVPLESEIGSNTQVGPTA
                     PHDIAMDIPQHEPFLPEPLAAPSIGILQVPIPSTVFLILTGLIGLMVRSAKFRSLRG"
     gene            complement(3793..>4652)
                     /locus_tag="VFP93_04665"
     CDS             complement(3793..>4652)
                     /locus_tag="VFP93_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9843929.1"
                     /translation="RDLRLSGIVFTGSTNTAWHINQQLALRKGALIPFIAETGGQNAM
                     IVDSTALAEQVVADVIASIFRSAGQRCSSLRVLYLQEEIFDKVTTMLKGAMLELKVGN
                     PIQIDTDVGPVIDANAQKGLYQHLAWLDQHGQKICKLDLTEVCKEGYFVPPSVYEIQS
                     MEQLKQENFGPILHVIKFARKNLDRVLHEIENSGFGLTLGIHSRIDETIAYITHKARV
                     GNIYVNRNMIGAVVGVQPFGGERLSGTGPKAGGPNYIPRLATERTVSINTTAAGGNAS
                     LMALNDDTI"
BASE COUNT         1369 a          902 c          908 g         1473 t
ORIGIN      
        1 cagcaagggg cacctttaga atacgttcgg gccttatttg atcaatatgc atttaattat
       61 gatacccatg ttaaaactat gcttaactat caagttcctg agaaaatgcg ccaagctatt
      121 gcgccctttg ctaccaaagc aacaaaagcg tgggatgtgc tagatttagg ttgtggtaca
      181 ggtctatgtg ctccctactt caccgatgtc gccggaaaaa tggtaggtgt tgatatttcg
      241 cccaatatga ttgaggtggc gaatcaaaaa gggggttatc ataaactcca cgtcgaggat
      301 gctttaaatt tcttagctaa aacgcaatat acgtatgatt taatcattgc ggcggatgta
      361 tttgtctatt ttgcagaact gcatgctatt ttgtcggcaa tagggttaaa attagttcca
      421 cagggttttt gcatattctc tattgagaaa tgtgaagata gtgaaggccc ttttatttta
      481 cgcaaaacag gacgttatgc ccatagtgct tcttatttac aaacattatg tgcccaatta
      541 catttgttgg tactgtttca agaagaaaca attttacgta aacaagagga tcaagatgtt
      601 tatggctata taataatcgt gcaaaaaatt tctgtgtagc cacaccttaa attctatttt
      661 ttcacgatat actatatagt attctagctg gagataatta tggaatggct tcaagaacta
      721 cattttttgc attacattct cattgggttt ggtttagtcg tgctctttta cttcatggtt
      781 gtttacaaca gtctagtagc attaaaaaat aacgtaaaaa aggcttgggc taatatcgac
      841 gtactcttaa aacaacgtca tgaagaactc cctaaactgg tcaccacctg caaacaatac
      901 atgggctatg agcgggatgt tttggaaaaa attaccgagc tacgcaatac cgtcaaccaa
      961 atgcgtacct cgggagacat tttagggtta ggctcagcag aatcacatct acgcgatgcg
     1021 ctgggacgat tatttgcttt atcggaaaat tatccggatc ttaaggctga ttccactttt
     1081 ttgcatctgc aagcgcgtat ttcgagttta gaaaatgcta ttgccgatcg acgcgaactc
     1141 tataatgaaa atgccaccct caataatatt cggattgaac aatttccgga tattgtggtg
     1201 gcatttattt ttagatttaa acatcaaccc atgttagagt ttcaagtaga agaactaggt
     1261 cctgtagata tgaatgcttt atttaaagat tagctatgca ttcacactat caatcaaact
     1321 acgatttaga tttaacctta ctttgcgaaa tcccagaaat gtggtctttt ggagtgctat
     1381 ctatctttgt cgttttttgt attggtttct tttacgcctt ttataaaaat ttgctgcgcg
     1441 cgcatgcagt acgcgacact cccacttcaa aaattcgttc tgcacaccaa ggttatgtgg
     1501 aattaatggg aacggtaggc aaaagtcctg aaggtacttt acagggtgta ctttctaagc
     1561 ttccttgcgc ttggtatcaa tataaaatag aacattatca tgacaaaaaa tggacagttt
     1621 tagaacaagg atatagccaa aatatactgg ttttagaaga caccacgggt ttatgctatg
     1681 tggatccgct aaaagcgcat atcactaccc attcttactc gcgttggcaa ggtactaaac
     1741 gataccccaa tcgtatgccc caaggttttt ggcaaaattt attttggaag agtggtcctt
     1801 atcgttatac cgaatggcgt atggaagaag gaatgccttt ttacgcactt gggcattttg
     1861 tgactttctc taaagcggct ggattagaag tgaatgcgca aaacgtgcag caacttttaa
     1921 aaaaatggga aaaaaagcat aaaactttat tggcgcaact gttagcacaa cacagtcatg
     1981 ctaccgcaaa tcaaacagaa aaaactttaa gttaccaagc taaaaaagaa atgttgcgtg
     2041 atgcttacga aaaagcccat tccactttta acgttctcag tggttacggc ttagatcggc
     2101 gtcaaccatt tattatttca accaaagaag taggtcgttt aagttctggt tatttttggg
     2161 atgcgatgct atgggcacta gcttttattt ttttatttgg cttaactgca agtgttatcg
     2221 gatttagaat ttcttgttaa atttttttcg ttttatcatg ggaattggct taaagttgcg
     2281 ttggtattct tcggaaaaac tcggcccttt ggcaagaaaa tgtgctaatt cctctaattt
     2341 tctactataa ccattactgg gcggaaaacg ctttttttgt gtttctagac aatgtataaa
     2401 attggaatat tgcaaaggtg gatctttaat aaaatggtaa agttcatggg taacttcagc
     2461 ccaacttacc cattgaatat ggtgcaacct actgccaaac caacggtcaa aacggtattt
     2521 acccgtacca tggagataag cataaatttc ggtaattatt tggtctaata attcgtcacc
     2581 gtcgaaatgt atgcctttta atcccatggg aatctccaag atatcctctc cttggagtta
     2641 cttaaaatta gtttatcatt ataagttgct attctcaatt ttatatcttt aaaacatgca
     2701 ttaacatttt tttctaaaaa ttatgcgatg tagtgatcta cacttgtatt cttaaaaagc
     2761 aaactatatt tttttgggtt gaatggttaa cttcaatttt atgcgatggc gaatatttcg
     2821 ttattattcg ctaaaatctc gatacaacaa aaaatttaat ttttctgttc tttccaaaaa
     2881 aagcatgcta ttgtttattt gggatttata tttagaaaat catcatggag ggtggtatgt
     2941 cccatttggc gacaaagcta aaattattta ttgttctcag cctttgttcg caggtgagcc
     3001 atgcactcat catacattcc gacccctcgc acagcctcga aaatttgggt gatttccaag
     3061 ccgaagtaca ttgggatggt ctaggtctca atatacaact taccaacctt tccgaaccaa
     3121 ccaatggtgg ctacattact gggtttctaa tgaatctacc cggcgacaca actttccaat
     3181 atgccacaac agattcgaaa ttttcacctt taatgccaga aaaaggccca tactacggta
     3241 ctaaaccata tggttatttc gattatggtt ttacactggg cggtagtttt ctagggggtg
     3301 gcaaacccca taaaggtggg attggtgttg gccaaagtgc caattttacg ctggcaggat
     3361 ggttaaactt tccggatgat ttggatataa ataatgcgga cgttgtagaa aattattttt
     3421 ttgacgcatt aaaatcgcca gaattaggca gcataagctt tctcacacga tttcgcggtt
     3481 ttgaagacga aggcagcgac aaagtgcccg gtgtagcgca gcttcatgat ctcaattttg
     3541 tgcctctaga atctgagata ggatcaaata cccaggttgg gcctactgca cctcacgata
     3601 tagcaatgga tattccacaa catgaaccat ttttaccaga accattagcg gctccgagta
     3661 ttggaatttt acaagttcct attcctagta cggttttcct aattttaacc ggtttgattg
     3721 gcttaatggt gcgaagtgct aaatttagat cattgcgtgg gtaaatagga agtctaaaaa
     3781 tgatgacact ctttaaatag tgtcatcatt taaagccatt aaacttgcgt tgccaccagc
     3841 cgctgttgtg ttgatggata cagtacgctc ggttgctaga cgtggaatat aatttggacc
     3901 acctgctttg ggtccagttc cagacaaccg ttcaccgcca aaaggttgga caccaaccac
     3961 agcgcctatc atattacgat tgacataaat attaccaacg cgtgctttat gcgtaatata
     4021 agctatggtt tcatcgatcc gactatgaat acccaaggtt aggccaaatc cagaattttc
     4081 aatttcatgc aaaacgcgat ctaggttttt gcgagcaaat tttataacgt gtaaaatcgg
     4141 accaaaattt tcttgcttta gctgttccat agattgaatc tcataaacac tcggtggaac
     4201 aaaatagcct tctttgcaaa cttccgttaa gtcgagttta caaatttttt ggccgtgttg
     4261 atctagccat gctaaatgtt gataaagccc tttttgcgca ttagcgtcta tgactggccc
     4321 tacatcggtg tcgatttgta taggattccc cacttttagc tcaagcattg caccttttaa
     4381 catggtggta accttgtcaa aaatttcttc ctgcagatac aacacgcgca atgaagaaca
     4441 cctttgaccc gcacttcgaa aaatagaggc aatcacatct gcaaccactt gttctgcaag
     4501 cgctgttgag tcaactatca tggcattttg cccgccggtt tctgcaataa aaggaatgag
     4561 tgctcccttt cttagtgcta actgttggtt aatatgccac gcagtattcg tagagcccgt
     4621 aaatacaatg ccactgagtc gcaagtcacg cg
//
LOCUS       DASTIB010000270         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_72896, whole genome shotgun sequence.
ACCESSION   DASTIB010000270 DASTIB010000000
VERSION     DASTIB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72896"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1425..1781)
                     /gene="ssrA"
                     /locus_tag="VFP93_04670"
     tmRNA           complement(1425..1781)
                     /gene="ssrA"
                     /locus_tag="VFP93_04670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1833..2309)
                     /gene="smpB"
                     /locus_tag="VFP93_04675"
     CDS             complement(1833..2309)
                     /gene="smpB"
                     /locus_tag="VFP93_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET9843930.1"
                     /translation="MAKKKKDIQPDSTIAVNRKVRHEYSLEKFFEAGLVLQGWEVKSI
                     RAGKANISEAYIILKNGEAWIIGSQFVPLLSASTHVVAEANRTRKLLLHQSELNQLVA
                     AVERQGYTIVPLKLFWKNGRVKCDLAIAKGKKLHDKRQSIKDREWQRSKARVLKRN"
     gene            2392..2820
                     /locus_tag="VFP93_04680"
     CDS             2392..2820
                     /locus_tag="VFP93_04680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HET9843931.1"
                     /translation="MPVVRRKAIIPYTPSQIYSLVNDVSQYPNFLPWCNRVEILGHDH
                     HYMKARVFITQGIFKYSFTTMNYLTPHDTVQMALVEGPFKSLKGLWQFKELNSGTEVS
                     FFLDFEFASKWMELMLGPIFNQITDTMIGTFLKRAHELYG"
     gene            2813..>2977
                     /locus_tag="VFP93_04685"
     CDS             2813..>2977
                     /locus_tag="VFP93_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HET9843932.1"
                     /translation="MDKIQIEVAYASTTEHQTLLTLRVDSTCTVEKAILLSGILDIYP
                     EIDLSNQVVGI"
BASE COUNT          900 a          557 c          539 g          981 t
ORIGIN      
        1 ttccgctctc atttctgcaa tttttaaatt ttttcatttt atggaacaaa ggaagaagaa
       61 attaaaaaaa taatgtatgt ttagaatgaa gaattaaagt tccgctgttc catttttgcg
      121 aattttttaa aacgaaaatt agcattgatc aatcggatat aaaccctttt ccaattcaga
      181 gcaatgtaat aaaagagcaa aataattttt atacaaaatt tgtagggtgg tggcttttgt
      241 caaaatatca caatcctgct accagctaat catccgctat ataaaactct catcgtcctc
      301 gcaatatacg taaaatcacc atcaatgatt aatgaataga acgttagtag ctggagcttt
      361 taatggcgcg gagcctctgt tgaggttcat cttacaagcc tgctaatctt cattaagggc
      421 tttaacgtac aacttgaata atctttagaa ttcgtagcta gctgtttgcg gagagctgaa
      481 gcttacagag aaaatttaat ttagattaaa atcttttcaa aaatgcattt tttactgcaa
      541 gcaacttgaa agaagaaatt agattatcat cttgccaatt caaaaaactt tagatagtct
      601 aagtgacgtt atccacgtaa tttgtggatc gcgctccctt aagtggtgga acactcatga
      661 gagcacttac cattcaacta aatctgtgtt ggagattcag aaaaatgatc atcattattc
      721 taacgtaaac attttcaatt taaagtcaat ttcgataacc gaaagctata tggtgctaat
      781 tctttgtcta aagaaagttt ttagctcaaa attcttttgt attggttatc tgtcaattcc
      841 cttatcaaaa tggtaaggga tctaaaggtt tgcaggaact ggtgcttatg aatgaaaaaa
      901 taccgtatat tttgtttatc tgtaatacat ttataggttt aagattcttt agcaatttct
      961 ttgcacataa agattggaat cttttgatcc caatgttact ttgtgttttt gtttataact
     1021 ttcatgctct tatatatttt catgcggttt ttgatgccct atttttgctt tacatgagtg
     1081 tgttttcatt ttcgttggca gtgtatttgc atactcttat taaaatcact ctttgaagtt
     1141 taattgtgca agcaaagtat ctcgctgaaa gagtaatatg atggaatttt atagtgtggt
     1201 tggaatggat cccgcatatt aagcggcatt aatgctcgca agctcgctaa atgtcgctaa
     1261 attgcgggat gacgaatcat agagcacggt taagattacc ataagatttt ttcttgggat
     1321 cgctaacaaa acgggtataa atacggagat ttcgtcaaat agtgcctcta tagtgccgca
     1381 attcttgcgg ctgtctaaaa tacctttaaa atcagatatt tatatggtgg aggcggcggg
     1441 aattgaaccc gcgtccgcaa gccctaggct taaaggtcta catgtgtagc tgaatttttg
     1501 ttttaactag aacggcccaa cccagcaggg ggaactagcg agtctctctt tatttagcaa
     1561 tcgagtgccg agacaacctc ttttgcggtc ttgtgattgc gacctctgtt tccccgcgca
     1621 caagcacgca aagaacaaag gacagtagac taagctgcta ctgcgtatgt ttcgtcgttt
     1681 gcatttataa caaactgcag tctacttttt acgaggtaat tctgcataac tcgacatgca
     1741 ctttaagggt cgtgacctcc gtcgaagcca agatcgcccc caaaaataag ataacataaa
     1801 atctcaaaat ctaaccaatg cacatacact cgttaattgc gcttcaaaac ccgtgctttg
     1861 gagcgttgcc attccctatc tttaatagat tggcgcttgt cgtgtagctt cttgcctttg
     1921 gcaatggcaa gatcgcactt tacacggcca ttcttccaga ataacttcaa aggcacaata
     1981 gtatagcctt ggcgctctac cgctgccacc aattgattta actcagattg atgtaataat
     2041 aatttacggg ttctattggc ctctgctact acatgcgtag acgctgataa taatggcaca
     2101 aattgagagc ctattatcca agcttctcca ttttttaata taatataagc ttctgaaatg
     2161 ttggctttgc cagccctaat gctttttact tcccacccct gtaacactag acctgcttca
     2221 aaaaatttct ctaaagaata ttcgtgccgc acctttcggt taactgcaat ggtactatca
     2281 ggttggatgt ctttcttttt cttggccata agggaagcat tatacgatta cttttactgt
     2341 aaaataaagt gcaatctggt ataatgactt ataaattgtt taaatttttc tatgccagtc
     2401 gttcgacgaa aagcaatcat accctataca ccatctcaaa tttactcttt agtgaatgat
     2461 gtttcacagt atccaaattt tttgccctgg tgtaacaggg ttgaaatctt agggcacgac
     2521 caccattata tgaaagctcg agtttttata acacaaggca tatttaagta ttcttttact
     2581 accatgaact atttaactcc acatgatacc gtgcagatgg cattagttga gggtcctttt
     2641 aaaagtttaa aaggcctatg gcaattcaaa gaacttaatt cgggaacaga agtgagcttt
     2701 tttcttgact ttgaatttgc ctccaaatgg atggagttaa tgttagggcc aatttttaat
     2761 caaattactg acacaatgat aggaaccttt ttaaaaagag cacatgaact ttatggataa
     2821 aattcaaata gaagtagctt atgccagtac tacggagcac caaacgcttc ttacgttgcg
     2881 ggtggactct acttgcacag tagaaaaagc cattttgctt tcgggaattc ttgatatcta
     2941 tcccgaaatc gatttatcaa atcaagtggt tggtata
//
LOCUS       DASTIB010000271         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1064145, whole genome shotgun sequence.
ACCESSION   DASTIB010000271 DASTIB010000000
VERSION     DASTIB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064145"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFP93_04690"
     CDS             complement(<1..1175)
                     /locus_tag="VFP93_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9843933.1"
                     /translation="MKTTREILKLYANAHPFALTAIIIASFFWAVTWPASAFLLGILV
                     DTIQNTNQAALRNQSGSISSWLVNSFETIGWPVAAMIIINLLRNMVYFTIGVSFAKSI
                     PSSKASLIDKLFRYLVGQSYRYFENQYSGQLANKIVNAQSGLESAIQLLNLNIATLLA
                     LLISGALLASVSLFFTLLFWLWAISICYYSYRKALIGENKSKIYATSQSLLQGEMVDS
                     LSNMTTVVTDATGEYEIKRLNIFINQYIQEDRTLQSHFNIARLGHGLLTSLFIALMLI
                     GLLIGFYYQIVTPGDFAFILSLSWSMAGFVHDLGKQFLDLSQHLGRLSEGMSILQIPQ
                     NLKDKEDAKDYSLQEGSIKFDQVFFGYNHTNVFENFNVVIRSGEKVGIVGRSGCGKTT
                     "
     gene            complement(1825..>2083)
                     /locus_tag="VFP93_04695"
     CDS             complement(1825..>2083)
                     /locus_tag="VFP93_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843934.1"
                     /translation="KIVELKNYEKQIEILTEVEKNTKKLSASLDFVEKRKQHILKLLP
                     HVKHGEQIEKDLAALQVRIDEMNKNKPGSTELYFCKKGMSR"
BASE COUNT          658 a          360 c          365 g          700 t
ORIGIN      
        1 gtggttttgc cacaaccgga gcgacccaca attccaactt tttcacccga acgaatcact
       61 acattgaaat tttcgaaaac attcgtatga ttatatccaa aaaaaacttg gtcaaatttt
      121 atagaacctt cttgtaatga ataatctttt gcatcttctt tatctttaag attttgcggt
      181 atttgaagga tgctcattcc ttcagataat cgacctagat gttgggaaag atctaaaaat
      241 tgcttgccta aatcatgcac aaatcctgcc attgaccaac tcaatgaaag gataaatgca
      301 aaatccccag gagtaacaat ttgataatag aaaccgatta gcagtccaat cagcatcaat
      361 gcaataaaaa gggaagttaa taatccatgc cctaaacgcg ctatgttaaa atgggactgt
      421 aatgttcggt cttcttgaat gtattggtta ataaatatat ttaaacgttt aatctcatat
      481 tcacccgttg catcagtcac aactgtggtc atgttgctta ggctatcaac catttctcct
      541 tgcaaaaggc tttgacttgt tgcataaatt ttagacttat tctcacctat cagtgccttt
      601 cgataggaat aatagcaaat agatatcgcc cacagccaaa aaaggagtgt gaaaaataga
      661 ctaacgctcg ctaaaagagc gcctgagata agcagcgcaa gtaaagtggc aatatttaaa
      721 tttagcagtt gtatggcact ttccaatcca gattgggcat tgacaatttt attggcaagt
      781 tgaccactat attgattttc aaaatatcta taagattgtc ccacaagata gcgaaacaat
      841 ttgtcaataa gtgaagcttt ggatgaagga atagatttag cgaagcttac gccaatggta
      901 aaatacacca tattgcgcag aagattgatt ataatcattg cagcaactgg ccatccaatg
      961 gtttcaaaag agtttactaa ccaggaagaa atactgcctg attggttacg aagcgcagct
     1021 tgattggtat tttgaatagt gtcaactaaa atacctaaca aaaaagcact cgcaggccaa
     1081 gttaccgccc aaaagaatga agcgataatg atagccgtaa gcgcaaaagg atgtgcgttg
     1141 gcatatagct ttaatatttc acgtgtggtt ttcatgggct ttatcataat gagcatggga
     1201 ttgaatggat tgttattttc caaaatttga gttgatgttt ctaaccatta tcgtctaaca
     1261 catatacaat tcaatttctt aacaatactt catttaagca taatttatta ttgttcctgc
     1321 agctaacttt atccaaagag ctttattttt tgcaagatag ttgtcatgcc aagcagacaa
     1381 ttaacgtgag caactattta cttgtgtgaa tgtatttggt tgtttttgta caggggaaga
     1441 gtatttttta atcgagatct atattttagg ggattgtgat atttctggaa atcttccttt
     1501 ttgaatagtc tcccgacatt tatttgaaaa taactttaat gcgggggtca gtacaagtga
     1561 atgcgatcct aatgcagtag tcattatagt tccatcgaac caattatttt tatatcatct
     1621 ctccttaatt ttgttattaa atattgcgcg gtgaatagta ttacagagat actttattgt
     1681 caatgaactt gatgacgtga atttttaaat ttaaatgcaa attttttgca aatttacata
     1741 tctattaagc tttgatattt tataacaaat gagaaaaatc atgcaatttt gcgaacgccg
     1801 tctagacggc gtcgcaaagt tattttagcg gctcattcct tttttacaga aataaagttc
     1861 ggtgctaccg ggtttgtttt tgttcatttc atcgatacgc acttgtaaag cggctaaatc
     1921 cttctcgatt tgctcaccat gtttcacgtg aggtaacagt ttcaatatat gttgcttgcg
     1981 tttttctaca aaatctaacg aagcagataa tttttttgta ttcttttcaa cttccgtaag
     2041 gatttctatt tgcttctcat aatttttgag ttcaactatt ttg
//
LOCUS       DASTIB010000272         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_557630, whole genome shotgun sequence.
ACCESSION   DASTIB010000272 DASTIB010000000
VERSION     DASTIB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557630"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFP93_04700"
     CDS             <1..653
                     /locus_tag="VFP93_04700"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9843935.1"
                     /translation="IKNVGLQTAHLKAIHYFLNTLFFVQKDGPVGFNAFWQHAKASAT
                     LMQSLAQEAKHASLDPIIAYRCGLLHNIGLLLLGHLFPPEFCLLNKMLWQSKKSSIEM
                     LEKKLLGMGLAQKMFGCGHAWLGSKLLEYWQLPEPLIITAREHHHINYQGNYQEYVYL
                     QIIANSLLKHQNLGEGMVLTFPELPLHELGLSTSLLNEYLEKKSLFISEEEPIVEI"
     gene            714..1364
                     /gene="adk"
                     /locus_tag="VFP93_04705"
     CDS             714..1364
                     /gene="adk"
                     /locus_tag="VFP93_04705"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9843936.1"
                     /translation="MRLILLGPPGAGKGTQAQHIQEKYEIPTISTGEMLRQAAHDGSD
                     LGLQAKQIMASGRLVSDDIIIKLVQERIQKKDCERGFLLDGFPRTVTQAIALTNSSIV
                     IDYVIAMHAPDTIILERLSGRWIHAPSGRVYHEKTKPPVKRGVDDITGEPLVQRDDDK
                     ESTVRKRLEVYRVQTEPVIDYYKNLKGIIFVEIETTGTAQEISQQIDKIIGKVQWV"
     gene            1355..1726
                     /locus_tag="VFP93_04710"
     CDS             1355..1726
                     /locus_tag="VFP93_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9843937.1"
                     /translation="MGVIALTQKNFDEVTQTNHCVIVDFWAPWCSPCLTFAKIFEKLS
                     EKHPDIIFASVDIDKEPELADDFTIKAVPYLIIFREEFVIFAESGVQTEQGLEQLLAE
                     AKKIKIEDLRKQIQESPHSNS"
     gene            complement(2000..2416)
                     /locus_tag="VFP93_04715"
     CDS             complement(2000..2416)
                     /locus_tag="VFP93_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843938.1"
                     /translation="MLFFVAEDLRLAVPDALDFALLPFAELLVFFVVPRFAEVFLAEL
                     FFAPVLLEAPLVDFFAPVFFVAELLALVADDLALVVDDFFLALAPEDFFAPELLPEDF
                     DLDVLDFFIANLRFLFSCLRTLPALWNFQVKLRIYF"
     gene            2592..3287
                     /gene="tsaB"
                     /locus_tag="VFP93_04720"
     CDS             2592..3287
                     /gene="tsaB"
                     /locus_tag="VFP93_04720"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9843939.1"
                     /translation="MPKILSIDTSTEACSASLLIHNEMQVRFEICPRQHGVKILPMVD
                     DLLSSAQIALHNLDAIAVSQGPGSFTGVRLGISVAQGLSFGSDLPLVPVSTLKALAFG
                     AFHLASQADYIVAAMDAKMDEIYYAVYQTERQKTLKLVGEEQVIPPNFLPALPADFAT
                     KSIIGCGHGFKIYNETLCKRLGVPFVAKWPEALPNAENIGWLAKEAFLNHEIVSPQEC
                     MPVYLRNKVIQNI"
BASE COUNT         1123 a          642 c          696 g          959 t
ORIGIN      
        1 gtataaaaaa cgtagggtta caaacagcac atttaaaagc gatacattat tttttgaata
       61 cattattttt tgtgcaaaaa gatgggcccg taggttttaa tgccttttgg caacatgcca
      121 aagctagtgc gactctaatg caatcgttgg cgcaagaagc caaacatgct tccttagatc
      181 ctataatcgc ctatcgctgt ggcttgcttc ataacatagg cttattgtta ttgggccact
      241 tattccctcc ggaattttgt ttgcttaaca aaatgctgtg gcaatctaaa aaatcttcga
      301 ttgagatgtt agaaaaaaaa ttattaggta tgggcttagc ccaaaaaatg tttggatgtg
      361 ggcatgcctg gttaggcagc aaattactag aatattggca actgcccgag cctttaatta
      421 ttacggcgcg tgagcaccat catataaact atcagggtaa ttatcaggaa tatgtttatc
      481 tgcaaataat tgcgaactct ctcctgaagc atcaaaactt aggagagggg atggtgttaa
      541 catttccaga attaccgttg catgaattgg gtttgtctac aagcttactt aatgaatatc
      601 tagaaaaaaa gagcctattt atctctgaag aggaacctat tgttgagatt tagtgcccta
      661 catttcattt ctttatctga tatactttcc cactttagaa ggtggagtaa gttatgcgtt
      721 tgattttatt ggggcctccg ggtgctggaa aaggaaccca agcacagcat attcaagaaa
      781 aatacgaaat tcctacgatt tcaacaggcg aaatgttaag acaagccgcg cacgatggtt
      841 cagacctcgg attacaagcc aagcaaatta tggcaagtgg ccgtttagtt tcagacgata
      901 taattattaa actagtacaa gaacgcatac aaaaaaaaga ttgcgaacga ggatttttgc
      961 tggatggttt tccccgtacc gtaacgcaag ctatagcgct tacgaattct tctatagtaa
     1021 ttgactatgt tatcgcaatg catgctcctg acactattat tttagaaaga ttaagtgggc
     1081 gttggataca tgctccttcc gggcgtgttt accacgaaaa aactaaacca cccgtaaaac
     1141 ggggggtgga tgatataacg ggtgaaccgt tagtgcagcg cgatgatgac aaagaatcta
     1201 ctgttcgcaa acgacttgaa gtatatcgag tccaaacaga acctgttata gactattata
     1261 aaaaccttaa aggaataatt tttgtcgaaa tcgagactac gggcactgcg caggagattt
     1321 cgcaacaaat tgataaaatt attgggaagg tgcaatgggt gtaatcgctt taacccagaa
     1381 aaattttgat gaagttacac aaaccaacca ttgtgtaatt gtggattttt gggctccttg
     1441 gtgtagccca tgcctcactt ttgcaaaaat ttttgaaaaa ctgagtgaga aacatccaga
     1501 tattattttt gcttcggttg atattgataa agaaccagaa ctcgctgacg attttactat
     1561 taaggctgtg ccttatttaa ttatttttcg agaagaattt gtaatttttg ccgaaagtgg
     1621 ggttcaaacc gaacagggtt tagaacaatt attggcagag gctaaaaaaa taaaaataga
     1681 agacttgcga aaacaaatcc aagaatcccc gcattctaat tcataaaatt attctgaaca
     1741 aaaagtcagc attattaaaa taacttagat cgaaacaaaa atttatctct tttacacaca
     1801 agacgtaagc ctcatccctg cgaaccaaaa aaaaattatt ttctcaagaa attttttgaa
     1861 aaaatgtgaa aaatttttat aggtgtgcta tgttttcagt gtccgccctt ttgcaggaaa
     1921 gtgtcgttga agaaagataa cgagacttaa gaaataagag tcaggttaaa agtcttcacg
     1981 aatgtcggct aagatggaat caaaagtaga ttctaagttt tacttggaag ttccataaag
     2041 caggtaaagt tcgcaaacaa ctgaacagga agcgtaaatt agctatgaaa aagtcaagta
     2101 catctaagtc aaagtcctca ggtagaagtt ctggtgcaaa gaaatcttcc ggggcgagcg
     2161 ctaagaaaaa atcgtctaca accaaagcta aatcgtctgc aactaaagct aaaagctctg
     2221 caacaaaaaa aactggggca aagaaatcaa ccagaggagc ttctagcaaa accggtgcga
     2281 aaaaaagttc cgctaaaaaa acttcagcaa aacgcggtac aacaaagaaa acaagtagct
     2341 cagcaaaagg aagtagcgca aagtctaaag catcaggtac agctaagcgc aaatcttcag
     2401 ctacaaagaa aagcacgaaa gctgccggca caaaaaaaag aagtacgact cgcagaagta
     2461 ctaaaaaagc agcttaaagc atcttgataa aagccattgt tctacaatgg ctttttttct
     2521 atttaatagt tactcacctt attgaaaaca aagaatcttg ttgcgaatta tatctctcac
     2581 tataataccg catgccaaaa attttaagta ttgatacttc taccgaagcc tgttctgcat
     2641 cgcttttgat ccacaatgag atgcaagtcc gctttgaaat ttgcccaagg caacacggtg
     2701 tcaaaattct gcccatggtc gatgatttgc tttccagtgc gcaaattgcg ttacataatc
     2761 tggatgctat tgcggtgagc caagggccgg gtagttttac gggagttcgg ttaggtatta
     2821 gtgttgcaca agggttaagt ttcgggagtg atctgccctt ggttcctgta tctacattaa
     2881 aagctctagc atttggtgcc tttcatttag catcgcaggc cgactatatt gttgccgcta
     2941 tggatgcaaa aatggacgag atttattacg cggtatacca aacggaaaga caaaaaacac
     3001 taaaactcgt aggcgaagaa caagtaatac cccctaattt tcttccggct ttacccgcag
     3061 attttgcaac caaaagtatt attggttgtg gccacgggtt taaaatttat aatgaaacct
     3121 tatgtaaacg cttgggtgtt ccatttgttg ccaagtggcc tgaagcatta cccaatgcag
     3181 aaaacatagg ctggttagct aaggaagctt ttcttaatca cgaaattgtt tcaccccaag
     3241 aatgcatgcc tgtttattta cgcaataaag ttattcagaa tatttaaggc gttcggaatc
     3301 tttcggcgct acgttcgcga tggttccgta tacgcttcgc taggtttccg tcatcctcgt
     3361 gcgacgtgcg tttcgcacga agataggggt tccattctaa aaaatcccgc ataccgcttt
//
LOCUS       DASTIB010000273         7155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_184633, whole genome shotgun sequence.
ACCESSION   DASTIB010000273 DASTIB010000000
VERSION     DASTIB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184633"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFP93_04725"
     CDS             <1..493
                     /locus_tag="VFP93_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9843940.1"
                     /translation="MADGSDDAEDIVRFYRELQLGFDCVFGSRFLSKSLVVDYPLHKK
                     WINRLANHFIRILFHFRYNDITNAFKCYRREVIAGIQPLIAQHFNLTVELPLKAIIRG
                     YSYKVIPNAWYNRKYGVSKLKIREMGSRYLFIVLYCFIEKWLSRGDYHFSHALKKPYR
                     MPQ"
     gene            551..3127
                     /locus_tag="VFP93_04730"
     CDS             551..3127
                     /locus_tag="VFP93_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9843941.1"
                     /translation="MTATRRLALRLQQHLPQGYSKVYEAPAIFALSDWFQTLWNSYQI
                     RGATEDKILLTPFQELGLLNTLLQRSSHKVLLQLFPTAKLLRNTWKLLCQWQLDINTI
                     DFSQQEEGKFFYNLAHDFKEELKQRQWITSAELPNFLFQLIQNDQAQLVKNFSPSHCI
                     IFVGFDDYYPELLNFKHMMEALDWNIIFFQSNKQAKSIQRVVYQNFEQEIEAIARSAK
                     NAVQKKQSIGIVVPTLLENRTRIEDQFNKVFNEVSLLDPVWKISKFFNISAGLPLYAY
                     PVIADFFQSFSLIKEKIELDTLLGFLHSPFFLEGNETLCAQLSYHLKKESKKYWDHEE
                     VILLLSNLELEASESFIAFFEKIVLFKEKVLNNKNFSSCFDDLFFWIQLLNWPGPRTL
                     TSVEHQVVARFYNCIHEWHLLNLIINTITFEEAIDWLKRICIETTFQPESEVVPIHIM
                     GLLEASGQEFDQLYVTGLHGEILPALPSPNPFLPIGLQRALHMPHASSEREFIFAQSL
                     IESFLRSANEVNFSHHLNDAIYEFQDSALIAHIPSSGALDKNNLFLYELFFAENKTAT
                     CEPLMPFPLQEHEKVQGGTNVIRTQAMCPFQAFARYRLKAWEPEYFDDGITPIKRGIL
                     YHKALETIWKQLRNQQNLKKLSTHELEIFVTKIVDNLSNNRLLKDVSPIYWEVEKNSM
                     IILLLAWLELEKQRTDFKVLKQECLVQIHLKGLTLRAKIDRIDQSMEGIFIIDYKTSL
                     ISLSDTIGSRPKAVQLPLYCFADYPQNANGIFFAQITKEECLLRGVTSNTFESLKNAC
                     QHTDLSSLESWNDLLQYWRQTMDGLSLEFKEGHCKVEPLQPQSCKNCQLPGLCRIEKT
                     YI"
     gene            3124..6453
                     /locus_tag="VFP93_04735"
     CDS             3124..6453
                     /locus_tag="VFP93_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9843942.1"
                     /translation="MKDDFDQLDKKAREAALDPLRSFIVQAPAGSGKTQLLIQRYLKL
                     LSVVQRPEAILAVTFTRKAQQEMRQRIISQLQKAKGEKPTEEHEIKTWLLAKESLKRC
                     EQLRWNLLENPNRLKIMTLDAFCHHLVRRLPFLSQLGYDPEISEDPKAIYVKAIQAFL
                     LDVQRKESFLPALKKLLQHLDNSREKLIELLIKLLQKRDQWLKYIYPISKNSEQGLSI
                     LEEGLKNIVLGSLEKISNALSLQEKRYLLQEAQRSGEFVFRESADKPLARMREWQNFP
                     QVKIEDVAKWQLLKKWLLTEKKEIRKRFTKNEGFPVACTQKDKKVATLRKTEFEKFLN
                     QLQDSVFIEGLKSVEILPEGFTQQERIILTHIFELLPLLVAHVKVRFKEEGKVDFIEM
                     NEAALQALGSDDDVTDLGLSLDSQIHHILLDEFQDTSINQFLLLEKLVTSWSGQHDKS
                     LFLVGDPQQSIYGFRGAEVGLFLKVVQNGIQSIPLTFLQLRKNFRSSPSLIGWVNKLF
                     VDLFPVKSNITLGAVPYSASLSALKENINTEIQTLFFTHKDEEHRIITDIITQRLQAG
                     DKKIAILARTKDHFHRLIPWLRLWNIPFKATEILNLKHSLLIQDLRALLAATCDLSNR
                     VAWLSILRAPWCGLSIEDLWLIAHADEQPIIWDQLKNDAIIQTLTEEGQVNVLRLVKT
                     LNFWFSQKRRTDLSRALRSLWFTLGGNCCYSKEELPQAKLFFDVLYQHCQGGLLKNFE
                     QFNEKIESLFCSTEFSPQNQDAEVELMTIHKAKGLEFDTVIVPSLEKTTKNMDPELLL
                     WHELAHDNGTELLIAPVKEAGVSENTLYKYVQSILLEKNAFESQRLFYVACTRAKKNL
                     FLTATVSFDIQDNIKKPLHNSFLSYISSVETQVEFRKPQNINFTSAPAIRLSRDWEPP
                     RVYPELLPLINPTLPSLNRPIYAKTFESHIGILIHAILQNIAEEGLHKWNSSQLSKAQ
                     LAWRSWLTMQGITGSQLEQGLSFVMSAIQQTLNSPRGRWILSSHVQGKSEFRLIEKQH
                     PKDQIFIIDRTFLDDNIRWIIDYKVVFNPLTTLKIEDYLPQLFYYAKLMAHRENFPIR
                     CGLYFPFTDDWYETAVESIPA"
     gene            6514..>7155
                     /locus_tag="VFP93_04740"
     CDS             6514..>7155
                     /locus_tag="VFP93_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9843943.1"
                     /translation="MAAEKKQILGVFALAMINVAAIVSLRNLSIMVEYGFACVFYYVA
                     ATLCFFIPTALVCAELATGWPKAGGLYRWVSEAFTPEVGFLAVWYAWMLSIAWFPTVL
                     TFTASAIAYLIDPALIKNNHYMISVMLSIFWIATFINFRDMKTSSWVSSIGVILGTII
                     PGILIIGLGFVWLILGHPTKIETTWQSIIPEFNVGSMVFFAGVILSLAGMEMSA"
BASE COUNT         2310 a         1207 c         1351 g         2287 t
ORIGIN      
        1 aatggcagat ggatccgacg atgccgagga tatcgttcgc ttttatcggg aattgcaact
       61 aggttttgat tgtgtatttg gttcccgttt tttaagcaag agtttagtag tagattatcc
      121 cctgcataag aaatggatta atcgattagc aaaccatttt attcgcattc tgtttcattt
      181 tcgttacaat gatataacaa acgcatttaa atgttatcgg cgtgaagtta ttgcggggat
      241 acagcccctt atagcgcaac attttaatct aacagttgaa cttcccttaa aagctattat
      301 tcgtggttat tcctataaag tcataccgaa tgcatggtat aatcgcaaat atggtgtttc
      361 taaattaaaa attcgtgaaa tgggaagtcg ttacttgttt atcgttcttt attgttttat
      421 cgaaaaatgg ttgtcgcgcg gtgattatca cttttctcac gcattaaaaa aaccttatag
      481 aatgcctcaa tagttattta aactaataat gtacgatagc ttacgtaatc tatcatcgga
      541 atcgcttata ttgactgcga cgcgacgatt agcgctgcgg ttacaacaac atttacctca
      601 agggtattct aaagtttatg aagcgcctgc aatttttgca ttgagcgatt ggtttcaaac
      661 cctttggaat tcgtatcaaa ttcgtggtgc gacagaagat aaaattttac taaccccttt
      721 tcaagaactt ggactgctca atacgctttt acaacgttca tcgcataaag ttttattgca
      781 actatttccc actgcaaagt tattacgaaa tacgtggaaa cttttatgcc aatggcaatt
      841 ggatattaat accatcgatt tttcacaaca agaagaaggg aaattttttt ataatttagc
      901 acacgacttt aaagaggaat taaagcaaag acaatggata acgtcagcgg aactacccaa
      961 ttttcttttt caactaatac aaaacgatca agcgcaactg gtaaaaaatt ttagtccatc
     1021 gcattgtatt atttttgtag gatttgatga ttattatcct gagttactca attttaaaca
     1081 catgatggaa gcattagatt ggaacataat ttttttccaa tctaataagc aagcaaaaag
     1141 catacaacgc gttgtgtatc aaaacttcga gcaagagatt gaagctatcg cgcgttccgc
     1201 caaaaatgca gtacaaaaaa agcaaagcat tggaattgtc gttcccacat tactggaaaa
     1261 tcgcacgaga attgaagatc agtttaataa agtatttaac gaagtatctt tattagatcc
     1321 ggtatggaaa ataagtaaat ttttcaatat ttcagctgga ttacctttgt atgcttatcc
     1381 tgtgattgca gatttctttc aaagtttttc cttaataaaa gaaaaaatag agttagatac
     1441 cttgttaggg tttttgcatt ctcccttttt tttagaagga aacgaaacgc tttgtgccca
     1501 attaagttac catttaaaaa aagaatcaaa aaaatattgg gatcatgagg aagtcattct
     1561 tttattatcc aatttagaac ttgaagcatc tgagtctttt attgcgtttt ttgaaaagat
     1621 tgtacttttt aaagaaaaag tattgaataa taaaaatttt tcaagttgct ttgatgatct
     1681 atttttctgg atacaattat taaattggcc aggaccaaga acgcttacga gtgttgaaca
     1741 ccaagttgtt gcacgttttt ataattgcat acatgaatgg catttattaa atcttattat
     1801 aaataccata acttttgaag aagcgataga ttggttaaaa cgaatttgta tcgaaacaac
     1861 gtttcagcca gaaagcgaag tggtgccgat tcatattatg ggtttactag aggcgtcggg
     1921 ccaagagttt gatcaattgt atgttactgg cttacatgga gaaatccttc ctgcacttcc
     1981 cagtcctaat ccttttttgc cgattggttt acaacgcgct ttacatatgc cgcatgctag
     2041 ttccgaaaga gaatttattt tcgcacaaag cttaatagaa tcctttttac ggtctgccaa
     2101 cgaagttaat tttagtcatc atttgaacga tgctatttac gaatttcaag atagcgcatt
     2161 aatcgcgcat attccaagtt caggagcgct tgataaaaat aacttatttt tatatgaact
     2221 cttttttgct gaaaataaaa ccgctacttg tgaaccctta atgccttttc ctttgcaaga
     2281 acacgaaaaa gtacagggtg gtaccaatgt tataaggaca caggcaatgt gtccatttca
     2341 ggcattcgca cgctaccgtt taaaagcatg ggaacccgaa tattttgatg acggaattac
     2401 ccctattaaa cgaggtatac tgtaccataa ggcattggag actatttgga agcaattgcg
     2461 caatcagcaa aatctaaaaa agctttcaac tcatgaatta gaaatttttg tcacgaaaat
     2521 agtagataat ttatcgaata atcgactatt aaaagatgtt tctcctatct actgggaagt
     2581 ggaaaagaat agcatgataa ttcttctact tgcttggttg gaacttgaaa agcagcgcac
     2641 agactttaaa gttttaaaac aagaatgctt agtgcaaatt catttaaaag gattaaccct
     2701 gcgtgccaaa atcgatagaa ttgatcaaag tatggagggc atttttatta ttgattataa
     2761 aacttcccta atatcgctct ccgatacgat tggcagcaga cctaaagcag tgcaattacc
     2821 actctattgt tttgcggatt atccgcaaaa tgcgaatgga atcttttttg cacaaattac
     2881 taaagaagaa tgtttattac gtggagtcac gtcaaataca ttcgagagtt taaaaaacgc
     2941 ttgtcaacat accgatttat cttctctaga atcctggaat gatttgttac agtattggcg
     3001 tcaaacgatg gatggcttaa gcttggaatt taaagaaggc cattgcaagg tggaacctct
     3061 gcaaccacaa tcctgtaaaa attgtcaact tcctggtttg tgtagaatcg aaaaaactta
     3121 tatatgaaag atgactttga tcaattggac aaaaaagcac gtgaagctgc tttagatccg
     3181 cttcggtcat ttattgtgca ggcacctgcg ggctcaggaa aaacacaatt gttgattcaa
     3241 cggtatttga aattattaag cgtagttcaa agacctgaag caatacttgc agtaaccttt
     3301 acccgaaaag cacaacagga aatgcgtcaa cggattattt cacaattaca gaaagcgaaa
     3361 ggtgaaaaac ctacggaaga acacgagatt aaaacttggc ttttagccaa agaaagttta
     3421 aaacgctgcg agcaactccg ttggaattta ttggaaaatc cgaatcgact aaaaattatg
     3481 accttggatg cattttgtca tcatttagtt cgaagacttc cttttttatc gcagcttgga
     3541 tatgatcctg agattagtga agatccaaaa gcaatctatg tgaaggcaat tcaggctttt
     3601 ttactcgatg tccaaagaaa agaatcattt ctacctgcat taaaaaaatt gttgcaacat
     3661 ttagacaata gtcgtgaaaa actcatagaa ttacttatta agctccttca aaaaagagat
     3721 caatggttaa agtacatcta tcctatatct aaaaattctg agcaaggttt atctatttta
     3781 gaagagggat taaaaaatat tgtgttaggt tcgttagaaa aaatttctaa tgcactttct
     3841 ttgcaagaaa agcgttatct attgcaagaa gcgcaacgtt cgggagaatt tgtgtttagg
     3901 gaatctgcgg ataaaccttt ggcacgtatg cgcgaatggc agaactttcc gcaagtaaaa
     3961 atagaagatg ttgctaaatg gcaattgctc aaaaaatggc tccttacgga aaaaaaagaa
     4021 attcgaaagc ggtttacaaa aaatgaaggc tttccggttg catgcaccca aaaagataaa
     4081 aaagtcgcga cgctgagaaa gactgaattt gaaaaatttc ttaatcaatt gcaagactca
     4141 gtattcatag agggtttaaa atcggtagaa atccttcccg aaggctttac ccaacaagaa
     4201 cgcattattt taacccatat ttttgaattg ttaccactat tggtcgcaca tgtaaaagtg
     4261 cgttttaaag aagagggtaa agttgatttc attgaaatga atgaagcggc gttgcaagct
     4321 ttgggaagtg atgatgatgt aaccgatctt ggtttaagtc tagactcaca aattcaccat
     4381 attttattgg atgaatttca agatacttct attaatcaat ttttgttatt ggaaaaatta
     4441 gtcacctctt ggtcagggca gcatgacaaa tcattatttt tagtgggtga tccacaacaa
     4501 tccatttatg gttttagagg tgctgaagta ggtttgtttt taaaagttgt ccaaaatggc
     4561 attcaaagca ttccattaac gtttttgcag ttgcgaaaaa attttcgctc gagcccaagt
     4621 ctgattggat gggttaataa actttttgtt gatttgtttc ccgtgaaatc taacattacc
     4681 ctaggagctg taccttatag cgccagtctt tcagccctca aagagaacat caacacagaa
     4741 attcaaacct tattttttac ccataaagat gaggaacata gaataattac ggacataatt
     4801 acgcaacgtc ttcaagcggg cgataaaaaa attgctattt tggctcgtac taaggatcat
     4861 tttcataggt taataccttg gcttcgatta tggaatattc ctttcaaagc aacggaaatt
     4921 ttaaatctaa aacacagttt attgattcaa gatttacgtg cgttattagc tgctacctgc
     4981 gatttatcga atagggtagc ttggttgtct attttgcgtg caccttggtg cggtttgtct
     5041 atagaagatt tgtggttaat agcacatgca gacgaacaac ctattatatg ggatcaatta
     5101 aaaaatgatg caattattca aacactcacg gaagagggtc aagtaaatgt cttacgctta
     5161 gtaaaaacgt taaacttttg gttttcgcaa aagagacgca cagatttgtc gcgcgcactg
     5221 agaagtttat ggtttacatt agggggcaat tgttgttatt ccaaagaaga gcttccccaa
     5281 gccaagcttt tttttgatgt tctataccaa cattgtcaag ggggattgct caagaatttt
     5341 gaacaattta acgaaaaaat agaaagctta ttttgtagca ctgaattctc acctcagaat
     5401 caggacgccg aggttgaact tatgaccatt cataaagcaa aaggtttaga gtttgatacg
     5461 gtgattgttc ctagtttaga aaaaacaaca aaaaatatgg atccagaatt attgttgtgg
     5521 catgagttgg cacatgataa cggaacggaa ctgttaattg cccctgtaaa agaagcaggt
     5581 gttagtgaaa atactttata taaatatgta caatctattc ttttagaaaa aaatgcattt
     5641 gaaagtcagc gtttatttta tgttgcttgt actcgagcga aaaagaattt atttttgaca
     5701 gcaactgttt ccttcgatat acaagataat ataaaaaaac ctttacataa tagtttttta
     5761 tcttatatat catcagttga aacgcaagtg gaatttagaa aaccgcaaaa cattaatttc
     5821 acatcagctc ctgcgatacg attatctcgc gattgggaac caccgagagt ataccctgag
     5881 cttttaccgc ttattaaccc cacattacct tcgttaaata ggcctatcta tgcgaaaact
     5941 tttgaatcac acattggtat tttaattcat gcaatattac aaaatattgc agaagaaggt
     6001 ttacacaaat ggaattcttc ccaattatca aaagcacaac tagcttggcg ttcatggctc
     6061 actatgcagg gtataacggg atcgcaattg gaacaaggct tgtcttttgt catgtccgca
     6121 atacagcaaa cgttgaatag tccaaggggt agatggatat tatccagcca tgttcaggga
     6181 aaaagtgaat ttcgattaat tgaaaagcaa cacccaaaag accaaatttt tatcatcgat
     6241 agaacatttt tggatgataa tatacgttgg ataatcgatt acaaggtggt gtttaatcca
     6301 ctaactacct tgaaaattga agattattta cctcagctct tttattatgc aaaactgatg
     6361 gcacataggg aaaatttccc cattcgttgt ggcctgtatt ttccttttac agatgattgg
     6421 tatgaaacag cggttgaatc gattccagca taattgtact aaaaagtact ataggtgtag
     6481 ttagtattat caatattttt ttggagcttt gaagtggcag cagaaaaaaa acaaatctta
     6541 ggcgtatttg ctttagccat gatcaatgtc gcggcaattg tttccttacg gaatttatcc
     6601 attatggttg agtatgggtt tgcttgcgtt ttttattatg ttgcagccac actttgtttt
     6661 tttattccaa ccgcattagt gtgtgctgaa ctcgccacgg gttggcccaa agctggggga
     6721 ttgtatagat gggtttctga agctttcaca cctgaagtag gctttttagc cgtatggtat
     6781 gcttggatgt tgagcattgc ttggttccca accgttctaa catttaccgc cagcgcaatt
     6841 gcttatctca ttgatccggc cttgataaaa aataatcatt atatgattag cgtgatgtta
     6901 tcgatttttt ggattgccac ctttatcaat tttagagata tgaaaacttc cagctgggtt
     6961 agtagtattg gtgttatttt aggaacaatt attccaggta ttttaatcat tggtctggga
     7021 ttcgtttggt taattttggg tcaccctaca aagattgaaa caacatggca atctattatc
     7081 cctgaattta acgttggcag catggtcttt tttgcgggtg taatcttaag tttagcggga
     7141 atggaaatgt ctgct
//
LOCUS       DASTIB010000274         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1092222, whole genome shotgun sequence.
ACCESSION   DASTIB010000274 DASTIB010000000
VERSION     DASTIB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092222"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFP93_04745"
     CDS             <1..352
                     /locus_tag="VFP93_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET9843944.1"
                     /translation="NQQSANAIPNLFARNERVILFFDTEFGPMALVLVGAMIVASIST
                     VFAGLVAPNKIMLERLYSHTLEEKMSFKKGEEIAYFHLGSTVVALLGNKNIQWASSVT
                     NAHSLLLGQLLAHV"
     gene            437..1753
                     /locus_tag="VFP93_04750"
     CDS             437..1753
                     /locus_tag="VFP93_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HET9843945.1"
                     /translation="MTKKKVDENYLEKIRKISDAVVKAQMPIRILDIIKWPEHVKHKF
                     FENKCKQQPEITVADYHQNLKFDIRKKAEEFLDIEQMIIQQLGQFNTVGGILRRMCQE
                     YSTVLRMLNACGTSEFSKLSKLLYGSPKDVFHEGDPTITQLGSVMASALTKMGKSKHL
                     QEDPRNISAEKAVAMLQTRLNRHFHESDKTLRVLLDDGIISDAAAGADYIKIRQDAWF
                     NKRDIRILEVHEGFVHIATTLNGKLQPYCTFLSKGPPSSTITQEGLAVLMEIISLSSF
                     PARLKKIANRIRAIELAEDGATFLEVFNYFRQENFDDETAYVFTARVFRGSLPTLGPF
                     TKDLSYSKGFVTVYNFLQLAIKKGELHLIPLLFCGKTKLEDLRDLYQLQKQGIVKRAK
                     FLPPQIKDLNAICAWMCFTSFINTLTSEKIESDYDYLFSHKNMLRQ"
     gene            1759..>2243
                     /locus_tag="VFP93_04755"
     CDS             1759..>2243
                     /locus_tag="VFP93_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HET9843946.1"
                     /translation="MIPQKNPIGFEEWIALPELNLPAIKAKTDTGAQTSALHAFMVEK
                     FEDDEGIEKIKFGIHPIPEQPNIAVFCEANLVDERKIISSNGKMELRYIIRTLATFGE
                     HSWPIEISLTNRETMNYRMLIGRSAMEGKLVVVPDASFLLGKLDIEAYKDLKTKPHER
                     K"
BASE COUNT          730 a          395 c          443 g          675 t
ORIGIN      
        1 taatcaacaa agtgccaatg cgatccccaa tctgttcgca agaaacgagc gggttatttt
       61 attttttgat acagaatttg gaccgatggc tttggtattg gttggcgcca tgatagtagc
      121 gagcattagt accgtgtttg caggtcttgt tgcacccaat aaaattatgt tggaacgttt
      181 gtatagccat acgcttgaag aaaaaatgtc tttcaaaaaa ggagaggaaa tagcctattt
      241 tcatctggga tctaccgttg tggcattatt aggaaataaa aatatccaat gggcgtcttc
      301 tgttaccaac gcgcattcac tactattggg tcaattatta gcccatgtgt aacctctaaa
      361 acgtaataac tcgacaagcc ttttttacaa cgactatgat taaaaagagt tcgtggatta
      421 taatttttta tgttatatga ctaagaaaaa ggtagatgaa aattatttag aaaaaattcg
      481 gaaaatttcc gatgcagtcg ttaaagcgca aatgcccatt cgtattttag atatcatcaa
      541 atggcctgag cacgttaaac acaaattttt tgaaaataaa tgcaaacaac aaccagaaat
      601 tacggtagca gattatcatc aaaatctaaa atttgatatt cgtaaaaaag cggaagaatt
      661 tttagatatt gaacaaatga ttattcaaca attaggacaa ttcaatacag taggcggtat
      721 cttgcgccgt atgtgtcaag aatatagtac ggtgcttcgt atgttaaacg catgcggcac
      781 cagtgagttt tcaaaacttt ctaagttact ttatggttcc cccaaagacg tgtttcacga
      841 aggcgatccc acgattaccc aactgggatc ggttatggca agtgccttaa caaaaatggg
      901 taaaagtaaa catttacaag aagaccctcg taatattagt gcagagaaag cggtggccat
      961 gttgcaaaca cggttgaatc ggcattttca cgaatccgat aaaacattac gcgttttgct
     1021 agacgatggt attatttcag acgcagcagc aggtgcagac tatataaaaa ttcgtcaaga
     1081 tgcttggttt aataaaagag atatacgcat tctggaagtt catgaaggtt ttgtacacat
     1141 cgctacaaca cttaatggga aactgcagcc ctattgtact tttctcagta aagggcctcc
     1201 ttcttccacc attacccaag aaggtctcgc agtcttaatg gaaattatta gtttatcttc
     1261 tttccctgcc cgtttgaaaa aaatcgccaa tcgcattcgg gcgatagagc ttgccgaaga
     1321 tggtgcgact tttctagagg tatttaatta ctttcgtcaa gagaattttg atgatgaaac
     1381 ggcttatgtc tttacggctc gtgtctttcg aggtagtttg cctacactag gaccttttac
     1441 caaagactta tcttattcga aaggttttgt tacagtttat aattttttac agttagcgat
     1501 aaaaaaaggc gagcttcatc tgatcccttt gcttttttgc ggtaaaacaa aactcgagga
     1561 cttacgcgat ttataccaat tacaaaagca agggattgta aaaagagcaa aatttttgcc
     1621 gccacaaatt aaagatctca atgcaatttg tgcttggatg tgttttacca gttttattaa
     1681 tacactcact tccgagaaaa ttgaaagtga ttatgactat ttattttccc ataaaaatat
     1741 gttgcgtcaa taaattttat gattccacag aaaaacccca tcggctttga agaatggatt
     1801 gcattaccag aactgaattt gcctgccata aaagcaaaaa cggatacagg tgcacaaaca
     1861 tcggctttac acgcatttat ggtagaaaaa tttgaagatg atgagggcat tgaaaaaata
     1921 aagtttggaa ttcatcctat tcctgagcaa ccgaatattg cagtattttg cgaagccaat
     1981 cttgtggatg aacgtaaaat tataagttcc aatggcaaaa tggaattgcg ttatattatt
     2041 cgaacgctgg caacatttgg agagcattct tggcctatcg agatatcgct tacgaatcga
     2101 gaaaccatga attatcgaat gttgattggc cgatctgcca tggagggtaa attagtcgtt
     2161 gtgccggacg cttctttttt gttgggaaaa ctagatattg aagcgtataa ggatttaaaa
     2221 acaaagccac atgaacgaaa att
//
LOCUS       DASTIB010000275         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_242802, whole genome shotgun sequence.
ACCESSION   DASTIB010000275 DASTIB010000000
VERSION     DASTIB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242802"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /gene="gatB"
                     /locus_tag="VFP93_04760"
     CDS             complement(<1..1171)
                     /gene="gatB"
                     /locus_tag="VFP93_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET9843947.1"
                     /translation="MQWETVIGLEVHAQLNTKSKIFSGAPTAYGAAPNTQACAIDLGL
                     PGVLPVLNLEAVTKAITFGLATHSTIAKRSVFARKNYFYPDLPKGYQISQYELPIVHS
                     GYLDIEVQGQIKRIGITRAHLEEDAGKSLHEDFHGQTGIDLNRAGTPLLEIVSEPDMR
                     SAEEAVTYLKALHALVRYLDICDGNMQEGSFRVDANVSIRPKGAPEFGTRTEMKNINS
                     FRYVEQAIHHEVARQIALIESGKKVIQQTRLFDPDKQETRPMREKEEANDYRYFPDPD
                     LLPVIVEESFIAQIQSSLPELPHQKRARFIESYKLSDYDAGVLTASRPLADYFESALR
                     ASENSDPKLVTNWVMGDLLGALNKVNLSIDQSPVSAQQLGELLSFISKDTISGKIA"
     gene            complement(1187..2641)
                     /gene="gatA"
                     /locus_tag="VFP93_04765"
     CDS             complement(1187..2641)
                     /gene="gatA"
                     /locus_tag="VFP93_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET9843948.1"
                     /translation="MYQKTLKELAEDLKSKKVSSVEITEYYLNRIKNLNSQINAFITV
                     TEETALQQAKEADKKFANNTATILTGLPLAHKDIFCTSGIKTSCASKMLDNFIAPYDA
                     TLVTRLNEQGMVLLGKTNMDEFAMGSSNETSFYGPVKNPWDLERVPGGSSGGSAAAVA
                     ARMVPVATGTDTGGSIRQPAALSGITGLKPTYGRVSRYGMIAFASSLDQAGPMTLNAE
                     DAALMLSGMAGFDPLDSTSVEKEVANYSASLHDSLKGKRIGIPKEYFEAGLNSDVANI
                     ILEAINILKSLGAQVKEISLPNSAFSVPVYYVVASAECSSNLSRYDGVRFGYRCNNPQ
                     NLEDLYKRSRSEGFGSEVKRRIMMGTYTLSAGYYDAYYLKAQKVRQLIADDFKKAFEL
                     VDCIVGPTSPTPAFKLGEKMSDPVSMYLNDIYTIATNLAGLPGISVPAGFVDGLPIGL
                     QLIGRHFEESTLLNIAHQFQQETDWHLQLPPMIA"
     gene            complement(2652..2960)
                     /gene="gatC"
                     /locus_tag="VFP93_04770"
     CDS             complement(2652..2960)
                     /gene="gatC"
                     /locus_tag="VFP93_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET9843949.1"
                     /translation="MSKFEEKDIQKVAHLARLSLEAHEITAYAENMRNILQLIEQMNQ
                     VNTEKVSPMAHPMENVRQRLRKDTITEPNLREQFQSIAPSTEAGLYLVPQVIEDLVDH
                     "
     gene            3108..>3215
                     /locus_tag="VFP93_04775"
     CDS             3108..>3215
                     /locus_tag="VFP93_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9843950.1"
                     /translation="MFNAIRGIFSNDLSIDLGTANTLVYVRGQGIVLDEP"
BASE COUNT          907 a          674 c          654 g          980 t
ORIGIN      
        1 tagcaatttt gccagaaatt gtgtctttac taataaagct tagtagttcg ccaagttgtt
       61 gggcagatac aggactttga tctatcgaca ggtttacttt attaagagct cccaataaat
      121 ctcccataac ccaatttgta accaattttg gatcggaatt ttcgctagct cgtaacgcag
      181 attcaaaata atccgccaat gggcgcgaag cagttaatac tccagcatcg tagtcactta
      241 atttatagct ttcgataaag cgtgctcttt tttgatgcgg taattcgggt aagctagatt
      301 gtatttgagc aataaaagat tcctctacaa ttacgggtag taaatctgga tcgggaaagt
      361 aacgatagtc gttggcttcc tctttttccc gcatgggtct ggtttcttgt ttatccggat
      421 caaataaacg cgtttgctgg atcacttttt taccggattc gataagagca atttggcgtg
      481 cgacttcatg atgaattgcc tgctctacat aacgaaaaga attaatattt ttcatttcgg
      541 tacgcgtacc aaattctggt gcgccttttg gtcggataga gacattcgca tccacccgga
      601 aagatccttc ttgcatatta ccgtcacaaa tatctaaata gcgtacgagc gcatgtaatg
      661 cttttaaata agtaactgct tcctctgcag agcgcatatc gggttcggag acaatttcta
      721 agagaggcgt accggcgcga tttaaatcga ttccggtttg accgtgaaaa tcttcgtgta
      781 aagatttccc tgcatcttct tcaagatgcg cgcgcgttat gccaatgcgt tttatttggc
      841 cttgcacttc aatatcaaga tatccactat gaacaatcgg taattcgtat tgtgaaattt
      901 gatacccttt gggcaaatcg ggataaaaat aatttttgcg tgcaaaaacc gatcgctttg
      961 cgatggtgct atgtgtcgcc aatccaaaag taatcgcttt tgtgacggct tctaaattta
     1021 ataccggcaa tacgcctggc aaacccagat caatagcgca tgcttgggtg ttgggcgctg
     1081 cgccataagc ggtaggagca ccggaaaaaa ttttcgattt tgtgtttaat tgcgcgtgaa
     1141 cttcaaggcc aataaccgtt tcccattgca tagataaaac tcctacttaa gcaatcattg
     1201 gtggtaattg taggtgccaa tcggtttctt gttgaaactg atgtgcaatg ttgagcaaag
     1261 tactttcttc aaagtgtctg cctatcaatt gcaatccaat tggaaggcca tccacaaaac
     1321 ccgctggaac actaatacca gggagccctg caagatttgt tgcaatagta taaatatcgt
     1381 ttagatacat actcacggga tcggacatct tttcacctaa cttgaatgca ggtgtagggc
     1441 ttgttggtcc aacaatacaa tcgactaatt caaaagcttt tttgaaatcg tctgcaatta
     1501 gctggcgtac tttttgtgct tttaagtaat acgcatcgta atatccggcc gacaaagtat
     1561 aagttcccat catgattcgg cgtttgactt ccgagccaaa accttcggat cttgagcgct
     1621 tgtacaagtc ttctaaattt tgcggattat tgcagcgata gccaaaacgt accccatcgt
     1681 agcgcgaaag attggacgaa cactccgcag aggcaacaac ataataaacc ggcacggaaa
     1741 aagcactatt gggcaaggaa atttctttca cttgcgcgcc tagacttttc aaaatattaa
     1801 tggcttctaa aataatattg gcaacatcgg aatttaaacc tgcttcgaaa tattcttttg
     1861 gtatgccaat acgctttcct tttaacgaat catgtaaaga agcactataa ttcgcaactt
     1921 ctttttccac actcgtggaa tcaagcggat caaaacctgc cataccagac aacatcaggg
     1981 cagcatcttc tgcgttaagt gtcatagggc ctgcttggtc taaactcgaa gcaaaggcaa
     2041 tcatgccata gcgcgaaacc ctaccatagg tgggctttaa gccggtaatt ccgcttaacg
     2101 ctgcaggttg gcgaatagat cccccggtat cggtaccggt agctaccggc accatgcgag
     2161 cggcaactgc cgcggcggat ccacccgaag aaccaccggg aacgcgttct agatcccacg
     2221 gattttttac gggaccataa aagcttgttt cgttcgatga acccatagcg aattcatcca
     2281 tattggtttt tcctaaaagc accatgcctt gctcatttaa tctggtaacg agtgtggcat
     2341 cgtatggtgc aataaaatta tctaacattt tagatgcgca agaagtttta atgcctgaag
     2401 tgcaaaaaat atctttgtgt gccaaaggta atccggtgag gatggtagcg gtattgtttg
     2461 caaatttttt atccgcttcc ttggcttgtt gtaaggcagt ttcttcggta accgtaataa
     2521 aagcatttat ctgcgaattt aaatttttaa ttctatttaa ataatattcg gtaatttcaa
     2581 cactggatac ttttttactt tttaaatctt ctgccaattc ttttaatgtt ttttggtaca
     2641 tgctcaaatc ctcaatgatc cactaaatcc tcaattactt gaggtactaa ataaagacct
     2701 gcttcagtgg aaggggcaat actttgaaat tgctcacgca aatttggttc ggtgattgta
     2761 tccttacgta atcgctggcg tacattttcc ataggatgag ccataggaga tactttttcg
     2821 gtattaactt gattcatttg ttcgattaac tgcaaaatat tgcgcatatt ttctgcatag
     2881 gcagtaattt catgtgcctc aagacttaac cgcgcaagat gtgccacttt ttggatatct
     2941 ttttcttcaa atttcgacat gaaaccctct ttgacaatag gaattgtcaa ctagcttact
     3001 taaagaaata ctataaacca cactctatca tagagctgag cttggccaaa actacccatt
     3061 ttgcgatatg ctataaggat agaatctagg gacggagata gggatccatg tttaacgcga
     3121 tcagagggat tttctcaaac gatttatcta tcgatttagg cactgcaaat acgttggtat
     3181 atgtacgtgg ccaagggatt gtgctcgacg aacct
//
LOCUS       DASTIB010000276         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_185514, whole genome shotgun sequence.
ACCESSION   DASTIB010000276 DASTIB010000000
VERSION     DASTIB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185514"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFP93_04780"
     CDS             <1..530
                     /locus_tag="VFP93_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET9843951.1"
                     /translation="SFKLKELQKKYNFLRKGMYVVDLGAAPGGWSQVAVEYILPNGKL
                     WALDLLEMTPIPNVQFIQGDFTDPKIVDTLIQAMGVETKVDWILSDLAPNLTGLIDVD
                     QPKIIHLLESVFNFSNQYLKPQGGLLVKAFQGEGIDTYVKSLKQRFSQVLVRKPDSSR
                     AESREIYVLARGYNI"
     gene            641..>2493
                     /gene="ftsH"
                     /locus_tag="VFP93_04785"
     CDS             641..>2493
                     /gene="ftsH"
                     /locus_tag="VFP93_04785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9843952.1"
                     /translation="MNDMAKNLILWLIIAVILISVFSNFGTRQANINKIPYSQFLEYV
                     RSDNVQEVLIDDNQVRGQTRGDEPFISYIPQSVDPYLIAEVAQNKNVKILGKAREQQS
                     LLMSIFISWFPILLFIGVWMYFMRQMQGGGGRGALTFGKSRARMLGEDQVKVTFADVA
                     GVDEAKEEVKELVEFLKDPEKFQKLGGRIPRGVLMIGQPGTGKTLLAKAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNEGIIVIAATNRPDVLDKALLRPGRFDRQVVVGLPDIRGREQ
                     ILKVHMRRVPMAENVDAKIIARGTPGFSGADLANLVNEAALFAARHNKRVVDMEEFER
                     AKDKIMMGVERKSMVMSPQEKKLTAYHEAGHAIVGLSVPDHDPVYKVSIIPRAQALGI
                     TMFLPEADRYSYTRRRLESQIAALFGGRIAEEIIFGADHVTTGASNDIQRATEIARNM
                     VTRWGLSDKLGPLTYNEDEQEVFLGHSVGTRKEVSAPTASLIDQEIRNIIDRNYILAK
                     KILEENLEILHKMAQALIKFETISNDQIQDLMAGKEPREPSDWQPPPADSGT"
BASE COUNT          795 a          440 c          566 g          692 t
ORIGIN      
        1 caagttttaa attaaaagaa ctacaaaaaa aatataattt tctgcgaaaa ggcatgtatg
       61 ttgtagactt aggagcagct ccaggaggat ggtcgcaagt tgccgttgaa tatattctac
      121 ctaatgggaa gctttgggcc ttggatttgc tcgaaatgac ccccattcct aatgtacagt
      181 ttattcaagg ggattttacc gatccaaaaa tcgttgatac tttaattcaa gcaatgggtg
      241 ttgagacgaa agtcgattgg atattatcgg atctcgcacc aaacctcaca ggtctaatag
      301 atgtagatca accaaaaata attcatttat tagaaagtgt ttttaatttt tcaaatcaat
      361 atttaaagcc acaaggaggt cttttagtaa aagcctttca aggggaaggt attgatacct
      421 atgtaaaaag tttgaaacaa cgcttttctc aggtcttagt tagaaagccg gattcttctc
      481 gggctgaatc gagggagatc tatgtgctag cgagaggcta caatatatag taaggtgctg
      541 aaaacttaaa aacacatagt tgtgcaacgc aaaagaattc tggaagccga actctaggag
      601 gttcgataat agcttcccca aatttgataa gggttgagtt ttgaatgaca tggcaaaaaa
      661 tttaattctc tggctaatta ttgcagtaat tttgatatct gtcttttcta attttggtac
      721 tcgccaggcc aatattaata agattcctta ttctcaattc ttagaatatg ttcgttccga
      781 taatgttcaa gaagttttaa ttgacgataa tcaagtaagg ggtcaaactc gcggcgacga
      841 accttttata agttatatcc cacaatctgt ggatccttac cttatcgcag aagttgcaca
      901 aaataaaaat gtcaaaattt tgggtaaggc acgcgaacag caaagcttat taatgagtat
      961 ttttattagc tggtttccca ttcttctttt tattggtgta tggatgtatt tcatgcgtca
     1021 aatgcaagga ggtggtggtc gtggtgcttt aacatttgga aaaagtcgcg cacgcatgct
     1081 aggagaagat caagttaaag taaccttcgc agatgttgca ggtgtagacg aagcgaaaga
     1141 agaagttaaa gaattggtcg aatttttgaa agatccagaa aagtttcaaa agctaggggg
     1201 acgcattcca agaggcgttc ttatgatagg acaacccggt actggtaaaa ccctattggc
     1261 aaaagctatt gccggcgaag cgaaagtgcc ttttttcacc atttccggat cagactttgt
     1321 ggaaatgttt gttggtgtgg gtgcctctcg tgttcgagat atgtttgaac aagctaaaaa
     1381 gaacgcccca tgcataatat ttattgatga aatcgatgcc gtaggccgcc accgtggagc
     1441 aggtttaggc ggtgggcacg atgaaagaga acaaacgtta aatcaactac tcgttgaaat
     1501 ggatggattc gaaggtaatg aaggaatcat tgtgattgct gctaccaatc gtcccgacgt
     1561 attagataaa gctttattgc gacctggacg attcgatcga caagtagtag ttggattgcc
     1621 tgatattaga ggtcgtgagc aaattttaaa agttcatatg cgtcgcgttc caatggcgga
     1681 aaacgtcgat gccaaaatca tagcacgagg cacccctggc ttttccggtg cggatttagc
     1741 taaccttgta aatgaggcag cgttatttgc ggcacgacat aataaacgcg ttgttgacat
     1801 ggaagaattc gaacgcgcca aagataaaat catgatgggc gtagaaagaa aaagcatggt
     1861 catgagtcca caagagaaaa aattgactgc ttatcatgag gcaggacatg caattgtagg
     1921 attaagtgta ccggatcatg atccagttta taaagtaagc ataatccccc gtgcacaagc
     1981 tttagggatt acgatgttct taccagaagc agatcgctat agttatacac gaagacgtct
     2041 tgaaagccag attgctgctc tatttggtgg tcgaattgct gaggaaatta tttttggcgc
     2101 cgatcatgtt acgacaggtg ctagcaatga tatacagcgt gcaacagaaa ttgcgagaaa
     2161 tatggtcacg cgttggggat tgtctgacaa actaggaccc ttaacataca atgaagacga
     2221 acaagaagtc ttcctaggac attcggtagg aacgcgtaaa gaagtttccg cacccacggc
     2281 aagtcttatc gatcaagaaa tacgcaatat tatcgatcga aattatattt tggcgaagaa
     2341 aatccttgaa gaaaaccttg aaattttaca taaaatggca caagccctta taaagtttga
     2401 aaccattagt aatgatcaaa ttcaagattt aatggcaggt aaagaacccc gtgagccaag
     2461 tgattggcaa ccgccaccgg ctgactcagg aac
//
LOCUS       DASTIB010000277         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_247560, whole genome shotgun sequence.
ACCESSION   DASTIB010000277 DASTIB010000000
VERSION     DASTIB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247560"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="xrtA"
                     /locus_tag="VFP93_04790"
     CDS             complement(<1..793)
                     /gene="xrtA"
                     /locus_tag="VFP93_04790"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HET9843953.1"
                     /translation="MPKNWTINFKNDPLKVHAINFVIILLFFLYFYRSTLFTLIEKYA
                     TSGTYAHGFLILPIVLWLIFQRRHYFKISIPNYEPLGFIGILVLSLLWYVAGAAFVDI
                     IQQLCLCLMLIVLFISFFGLKISLKFLFPLAYLLFAIPFGNFLTPFLQHITTHVSAHI
                     LHVFGIPVYFEGLTISTSQGEFEVAEACSGIRYIIATVTLGVLYAYYTYQNIWKRIIF
                     SVICLVFPIIANCIRVFFIILIAHGSGMKYALGVDHLIYGWIFFGI"
     gene            complement(786..2003)
                     /locus_tag="VFP93_04795"
     CDS             complement(786..2003)
                     /locus_tag="VFP93_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET9843954.1"
                     /translation="MTGVLFLCHRIPYPPNKGDKIRSYHILKKLAEQYSVFVGTFIDD
                     PLDWNYVAKIQALATDSCILERTSLQKMILPAISTLFSTKPLTNKLYFSLQMRLWVEN
                     LIQSKSISKIVVFSVSMAQYVLKFHTTIPIVTDFVDVDSEKWSAYSQYRRFPLNIFCK
                     LEAKRLAAYETLVAQHSSTNIFVSPQEVNLFKKLSPSVTKPIMCVSNGVDSQFFNPNL
                     KYPNPYHGAKKRILFTGLMNYWPNVDAVSWFVKYVFTILAQQDKHIEFYIVGANPTKA
                     VFQLMRENIIVTGRVPDVRPYLKHADVVVAPLRVAQGLQNKVLEAMSMNKSIVASAQA
                     LEGIRVPKKGSIWVEITSKNWINRIENILYQIQDQPIDMHPWIVNHYSWENNLTSLSH
                     ILEGLNVQSDSHA"
     gene            complement(2000..>2193)
                     /locus_tag="VFP93_04800"
     CDS             complement(2000..>2193)
                     /locus_tag="VFP93_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation protein FemAB"
                     /protein_id="HET9843955.1"
                     /translation="FHFKRHWGFEAQTLPYRWILFTKKEKPNINPENPKYQWMIKCWQ
                     RLPLYVANTVGPLIARRLG"
BASE COUNT          765 a          397 c          379 g          652 t
ORIGIN      
        1 ctataccaaa aaagatccat ccatatatta aatgatcaac acccagcgca tacttcatcc
       61 ccgacccatg ggcgattaaa ataataaaaa aaactcgaat acaattggca ataatgggaa
      121 atactaaaca aattacagaa aaaataattc ttttccaaat attttggtaa gtataatagg
      181 cataaaggac acctaaggtt acggttgcaa taatatagcg aatgccactg cacgcttctg
      241 caacttcaaa ttctccttgg gaggtagaaa tggttaagcc ttcaaaatac accggaatgc
      301 caaatacatg taaaatgtgg gctgaaacat gtgtggtgat atgttgcaaa aatggtgtta
      361 aaaaattacc aaaagggatc gcaaataata aataagccaa tggaaataag aattttaaac
      421 ttatttttaa accaaagaaa ctgataaata acactatcaa catcagacac aaacacaatt
      481 gctgtataat atcaacaaac gcagcgcccg caacgtacca taataacgac aagacaagga
      541 tacctataaa acctaaaggc tcgtaatttg ggatgctaat tttaaagtag tgtcgtcgtt
      601 gaaaaattaa ccaaagcacg ataggcaaaa tgagaaatcc atgtgcgtag gtgcctgaag
      661 ttgcatattt ttcgatcaac gtaaaaagtg tggatcgata aaaatataaa aaaaataata
      721 ggataattac aaaattgatc gcatgcactt tgagtggatc atttttaaag tttattgtcc
      781 aatttttagg catgactatc cgattgaacg tttaagcctt ctaatatatg acttaaagac
      841 gttaaattat tctcccaaga atagtggtta acgatccaag gatgcatatc gattggctga
      901 tcctgtattt ggtaaagaat attttctatt cggttaatcc aattcttgga tgttatttca
      961 acccaaatcg aacccttttt tggcacgcgt atcccttcta gcgcttgtgc actcgcaaca
     1021 atcgatttgt tcatactcat tgcttctagc actttatttt gcaatccttg cgcaacacgt
     1081 aagggtgcaa caacaacatc cgcgtgtttc aaataaggtc ttacatcggg aacacgacct
     1141 gtaacaataa tattttcacg cattaactga aaaacagctt tggtcggatt agcacctaca
     1201 atataaaatt caatgtgttt atcttgttga gctaatatcg taaatacata ttttacaaac
     1261 caactgactg catctacatt aggccaatag ttcattaatc cagtaaataa aatccgtttt
     1321 tttgcaccat gataaggatt cggatatttt aaatttggat taaaaaactg actatcaact
     1381 ccattactta cgcacataat aggtttggta acactgggtg ataacttttt gaagagattc
     1441 acttcttggg gcgacacaaa gatattcgta gaagaatgct gtgcaaccaa agtctcataa
     1501 gcagctaatc ttttggcttc aagtttacaa aatatattaa gaggaaaccg tcgatattga
     1561 ctataagccg accatttttc tgaatctaca tctacaaaat cggttacaat gggaatagta
     1621 gtgtgaaatt taagcacgta ttgcgccata ctaaccgaaa atacaactat ttttgatata
     1681 gattttgact ggattaaatt ttctacccat aagcgcattt gcaagctaaa atacagttta
     1741 ttggtaaggg gtttagtaga aaacagcgtg ctaatagccg gaagaatcat tttttgtagg
     1801 cttgtacgtt caagtataca ggaatctgtg gccaatgctt gaattttggc aacataattc
     1861 cagtctaaag gatcgtctat aaacgtgccc acaaataccg aatattgctc ggcaagcttt
     1921 tttaaaatat gataagaccg aattttatca cccttattag gagggtatgg tattcgatgg
     1981 cacaaaaata aaacgccagt catcctagcc tccttgcgat gagaggaccc actgtgttag
     2041 caacataaag tggtaacctt tgccaacatt taatcatcca ttgatattta gggttctccg
     2101 gatttatatt cggtttttcc ttttttgtaa agagtatcca gcgatagggt aatgtttgtg
     2161 cttcgaaacc ccaatgtctt ttaaaatgaa aag
//
LOCUS       DASTIB010000278         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_825796, whole genome shotgun sequence.
ACCESSION   DASTIB010000278 DASTIB010000000
VERSION     DASTIB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825796"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFP93_04805"
     CDS             complement(<1..527)
                     /locus_tag="VFP93_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET9843956.1"
                     /translation="MEAMVYRKDLLDYLQSTLKPELFQDYGPNGLQVEGAEVVRKIIV
                     GVTANKALIQKAIESKADTLLVHHGIFWKNTPAPIIGVQYQRIKALMDYNINLIAYHL
                     PLDAHQTLGNHVQLAEKLGIKIEQFLDLENQPGLVVKGSIGQHTPQVFVRNVENVLQR
                     KPLYISGVNPNITQIA"
     gene            complement(539..1801)
                     /gene="murA"
                     /locus_tag="VFP93_04810"
     CDS             complement(539..1801)
                     /gene="murA"
                     /locus_tag="VFP93_04810"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9843957.1"
                     /translation="MEKHLIMGGTPLRGEVRISGAKNSVLPILTAGLLADEPIVIGNI
                     PHLNDVTTTMELLGQMGVKLTVLEGLTIEVDARRASNFVAPYDLVRTMRASILVLGPL
                     TSRFGYALVSFPGGCAIGPRPVDFHVEGLQKMGADIDIKGGYIEAKAPKGLKGATIDF
                     KVVSVTGTENLMMAATLAKGTTVLKNAACEPEVVDLAQMLNSIGAKIEGAGTKTIVIE
                     GVERLAGARYDVLPDRVETGTYLIAGAITGGKVTARNTRANLLVSVLEKLEEVGAKIT
                     TGEDFITLDMEGKRPKAVDITTEPFPGFPTDMQAQFMALNCVAEGQGIITENVFENRF
                     MHAGELRRLGADITIAGNQAIIKGTPFLTGAPVMATDLRASASLVLAGLVAKGTTLVD
                     RIYHIDRGYECIEEKLSQLGASIRRVPG"
     gene            complement(1806..2042)
                     /locus_tag="VFP93_04815"
     CDS             complement(1806..2042)
                     /locus_tag="VFP93_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HET9843958.1"
                     /translation="MDPNTLKSLIKKAIPDAEIFVEGDGYHYQTTVISDVFKDKSLVE
                     RQKLVYKAVSEHIQSGELHALTIKTKTTHEWGLK"
     gene            complement(2239..2538)
                     /locus_tag="VFP93_04820"
     CDS             complement(2239..2538)
                     /locus_tag="VFP93_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9843959.1"
                     /translation="MSTIKVTLQNNLFCYEGVLTFDTTLKALTETLKNFPKEGVVELD
                     LKNVSKSDSSGLALLTNLLSKAKEKNLHLRIKNTPKKMRDLARVSGLDELLPFSN"
     gene            complement(2540..3151)
                     /locus_tag="VFP93_04825"
     CDS             complement(2540..3151)
                     /locus_tag="VFP93_04825"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9843960.1"
                     /translation="MHLVVRSFVVLLLSLYACIALAQERPEVLLERVTQEMLTALKQN
                     DAEIKRNHAKLVEIIDKILVPHVDLHDMSRWVVGRNAWLKASASEKTRFAQEFKDLMI
                     RTYSSSLTAYSNQEIIYKPIRGSLEGKKRVQVSSQIIEPGRSPIQVSYRLVLKGGGWK
                     VYDIIIEGVSLLKGFKSQFEADIQEHGLEKVIEKMKTHNQKPR"
     gene            complement(3170..>3357)
                     /locus_tag="VFP93_04830"
     CDS             complement(3170..>3357)
                     /locus_tag="VFP93_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET9843961.1"
                     /translation="ILTAGLLGDNYIELDPGFADEEFESQLLVEEGHISKENTLSPIV
                     LEEIIAKFVSSEASGME"
BASE COUNT          974 a          722 c          622 g         1039 t
ORIGIN      
        1 gcgatttgtg taatatttgg gttaacaccc gatatatata aaggcttgcg ttgtagtaca
       61 ttttcaacgt tacgtacaaa aacttgtggc gtatgctgac caatgctccc ttttactaca
      121 agcccaggct gattttccag atcaagaaat tgctcaattt taatgcctaa tttctcggcc
      181 agttgcacgt gattgcctaa tgtttgatgc gcatcgagcg gtaaatgata ggcaattaag
      241 tttatattgt aatccattaa cgctttaatg cgttggtatt gcaccccgat tatgggtgcg
      301 ggagtatttt tccaaaaaat accatgatgc accaaaagtg tatcggcttt gctttcgatt
      361 gctttttgaa ttaacgcttt atttgcggta acacccacga tgatttttcg aacaacctct
      421 gccccttcaa cttgcaaacc attcggacca taatcttgaa aaagttctgg ttttaatgtg
      481 ctttgcaagt aatccagcaa gtctttgcga taaaccatcg cctccatatc gtttcctatt
      541 atccgggaac tcttcggata gatgcaccta gttgggaaag tttttcttca atacactcat
      601 aaccacgatc aatatgatag attctatcga ctagtgtggt tccctttgct actaatcctg
      661 caagtactaa actcgctgag gcacgcaagt ccgtggccat aacaggggct cccgttaaaa
      721 aaggagtgcc ttttataata gcttggttac cagcaatggt aatatccgca ccgagtcttc
      781 ttaattctcc ggcatgcata aaacgatttt caaaaacatt ttctgtaatg ataccctgtc
      841 cttccgccac gcaatttaat gccataaatt gggcttgcat atccgttgga aaaccaggga
      901 aaggctcggt cgtgatgtct acagctttag gtcttttacc ttccatgtct aacgtaataa
      961 aatcttctcc ggtggttatt tttgcaccta cttcttcgag tttttcgagt acagaaacaa
     1021 gaagattcgc gcgagtattt cgcgcagtga cttttccgcc cgtaattgcg ccagcaatta
     1081 agtaagttcc ggtttcaacg cgatcgggta aaacatcgta gcgcgcacca gccagtcttt
     1141 caacaccttc aatcacgatg gtttttgtgc cggcaccttc aattttggcg ccaatgctat
     1201 ttagcatttg tgctaaatcg acaacttcag gctcgcaagc cgcatttttc aatacggtag
     1261 ttcctttcgc caaagttgcg gccatcataa gattttcggt tccagtcacg gaaacaactt
     1321 taaaatcgat ggttgcacct tttaaacctt taggcgcttt tgcttcgata tagccgccct
     1381 ttatatcgat atcggctccc attttttgca agccttccac atgaaaatca accggtcttg
     1441 gccctatagc acaacctcca gggaaagata ccaatgcata accaaatcga ctggtcagag
     1501 gccccaaaac taaaatggat gcacgcatgg tgcggactaa atcataaggg gctacaaaat
     1561 tcgatgctct tcgcgcatcc acttcaatcg ttaaaccttc taataccgta agtttaacgc
     1621 ccatttggcc taaaagttcc atggtggtgg taacgtcatt taggtgggga atgttcccaa
     1681 taacaatagg ttcatccgcg agtaatcccg cagtcaatat aggaagaact gaattttttg
     1741 cgccagatat gcgaacttcg ccgcgtaagg gtgttccacc catgattaaa tgtttttcca
     1801 ttattttact tcaaacccca ttcatgcgta gttttggttt ttattgtgag agcatgtaat
     1861 tctccagact gaatatgctc acttactgct ttataaacca atttttggcg ttcgacgagg
     1921 gatttatctt taaaaacgtc tgaaatgacc gtagtttgat agtggtatcc atccccctct
     1981 acaaagattt cagcatcagg aattgctttt tttatcaaag atttaagtgt atttgggtcc
     2041 ataaagttgg aaaatcaaca tgctcaaaaa atcaatcttg cgctcatatt aatctttcag
     2101 cacaagaaag ggaatgttcg aagacacaac caatgttagt tgacaaaagt ttaacccgtt
     2161 gcattccaat tgaggaagag ctaatgcaga caataggtaa acaaaacata gcaagaaaaa
     2221 atataacgca agatagcact aattgctgaa aggaagcagt tcatccagcc cactcacacg
     2281 ggccaagtcg cgcatttttt tgggcgtatt tttaatacgt aggtgaagat ttttttcttt
     2341 ggctttactt aataaattcg tgagaagcgc aagaccagaa ctatcgcttt tagaaacatt
     2401 ttttagatcc aattccacaa caccttcttt gggaaaattt tttaatgttt ccgtaagggc
     2461 tttgagggta gtatcaaaag ttaatactcc ctcataacaa aataaattat tctgtaatgt
     2521 tactttaatc gttgacatat taacgcggct tttgattatg tgttttcatt ttttcaataa
     2581 ctttttccaa gccatgttct tgaatatcgg cttcaaattg tgatttaaac cctttgagca
     2641 aactaacacc ctcaataatg atgtcataaa ctttccagcc accaccttta agaactagac
     2701 ggtaagaaac ttgaataggt gagcgcccag gctcgataat ttgactagaa acttgcacac
     2761 gttttttgcc ttcgaggctg ccccgaatag gcttataaat gatttcttga ttagagtagg
     2821 ccgttaacga agaagagtaa gtacgaatca taagatcttt aaattcttgc gcaaaacgtg
     2881 ttttttcgga agcagaggct tttaaccacg catttcttcc cacaacccaa cgcgacatat
     2941 cgtgcaaatc gacatgcgga actaaaattt tatcgataat ttcaactaat tttgcgtgat
     3001 tgcgtttgat ttctgcatca ttttgtttca aggcggttaa catttcttgt gtgacgcgct
     3061 ctaaaaggac ttctggacgt tcctgcgcaa gcgcaataca agcatacaaa gacagcaata
     3121 aaacaacaaa tgagcgaaca acaagatgca tactattctc caaatataat tattccattc
     3181 cactagcttc agaggaaaca aattttgcaa taatttcttc taatacaata ggggataagg
     3241 tattttcttt tgaaatgtgg ccttcttcta cgagaagttg gctttcaaat tcctcgtctg
     3301 caaagccagg atctaattct atgtaattat cccctaataa acctgcagtt agaattt
//
LOCUS       DASTIB010000279         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1441758, whole genome shotgun sequence.
ACCESSION   DASTIB010000279 DASTIB010000000
VERSION     DASTIB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441758"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFP93_04835"
     CDS             <1..1003
                     /locus_tag="VFP93_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9843962.1"
                     /translation="IHTCDLSFSFYSRDLISYKGWNLGNPFSPRKNLFSKKSYQLFME
                     IIRFNNLGKNIVLLGTYKELFGDFLKKNAFTALFIEGYLYPLMTILWSCPFDNLYELP
                     TDFVLMYLKQNGLLETLGSPTWYFLRGGALAYTEPLTQDFRQNIHLNTPVIKILRDKY
                     HITLQTQHQAYTFDTLILATQADQALKLLQNPSPLERHLLSSFKYVTRNVYLHTDKTV
                     MPPQKQYWAALNYINKDKQLLTYHANRVQKFVSKMDYFITMHEGKPIIPGKIVIKTTL
                     NRPQYTAQNIANQARFNEINGILNTYYCGSYWGHGTLEDAVKSVYRALLPLQTVESL"
     gene            1031..1669
                     /gene="pdxH"
                     /locus_tag="VFP93_04840"
     CDS             1031..1669
                     /gene="pdxH"
                     /locus_tag="VFP93_04840"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET9843963.1"
                     /translation="MTNLNDLGRQYELSHLEEHDLKPQPLEQFQLWIQDAIEGKVKDP
                     NAMVLSTVNAQGLVSSRLMLLKYFDKNGLVFFTNYHSPKAQALQEHPQATLLFWWSEF
                     ERQVRIDGLVEKLDSEHSDAYFQKRERESNIAAMLSKQSTPLNDKKSFIDLFEKERNK
                     SSDDALIRPPFWGGYLLKPVYYEFWQGGPHRLHDRLVYRKIKEDWEITRLYP"
     gene            complement(1653..2504)
                     /locus_tag="VFP93_04845"
     CDS             complement(1653..2504)
                     /locus_tag="VFP93_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9843964.1"
                     /translation="MPFIIHRHRRLWYQDVGSGFPLLFGHGFLWDRKMWQPQIEILSQ
                     HYRCIVPDLWSHGASESVPQRPYSIELLSKDFYYLMDKLNLPEFAVIGQSISGMWGAQ
                     LSLNHANAVKALIMMDTFVGEEPIERKLMYSEILHSWEHKQHLSDEIIEKILPFFFTP
                     STFHTKPELIKDFKTKLKQLPVEQIEGLIEIGHTIFSRRSILDKLGLLKMPVLIMVGE
                     NDYPRPIHEAETMARYIPHAKLKIIADSGHVPSVEQPEIVTKILQNFLSEALADIVIP
                     AKIMDKV"
BASE COUNT         1002 a          530 c          501 g          939 t
ORIGIN      
        1 tattcatacc tgcgatttaa gttttagttt ttattcgaga gacttaatta gttataaagg
       61 atggaattta ggaaaccctt tttctccacg aaaaaatctt ttttcaaaaa aaagttacca
      121 gcttttcatg gagataattc gctttaataa tcttggtaaa aatattgtat tactgggaac
      181 ttataaagaa ttattcggag attttttaaa aaaaaatgct tttaccgcat tatttattga
      241 aggatacttg tatcctctca tgaccatttt gtggagttgc ccttttgata atctttatga
      301 gttaccgacc gactttgtat taatgtattt aaaacaaaac gggcttttag agactttagg
      361 atcgcctacg tggtacttct tgcgcggtgg tgctttagcc tataccgaac ctcttacaca
      421 agatttccga caaaatattc atctcaatac gcccgttatt aaaattttgc gtgacaaata
      481 tcacataacc ttgcaaaccc aacaccaagc ctatacgttc gatacactca ttttggccac
      541 ccaagcggat caagcattaa aacttttaca aaaccctagt cccctagagc gacatttgtt
      601 aagtagtttt aaatatgtaa cgcgtaatgt ttatttacat acagacaaaa ccgtaatgcc
      661 accgcaaaaa caatattggg cggccctgaa ttatatcaat aaagataaac aactacttac
      721 gtatcacgct aatagagtac aaaaattcgt ttcaaaaatg gattatttta tcaccatgca
      781 cgagggcaaa cctataatac ctggaaagat tgtgataaaa acaaccttga atcgtccaca
      841 atatacagcg cagaatatag caaaccaagc acgttttaac gaaattaacg gaattttaaa
      901 tacgtattac tgtggcagtt attgggggca tggaacttta gaagatgccg taaaaagtgt
      961 ttatcgcgca cttcttccct tgcaaactgt agaaagttta taatgatgca ttttaaaaaa
     1021 gagttgtggt atgactaatt taaatgatct cggacgtcaa tatgaattat cccatttaga
     1081 agaacacgat ttgaaaccac aacctcttga acaatttcaa ttgtggattc aagatgctat
     1141 tgaaggaaaa gtgaaagatc ccaatgctat ggttctctct accgtaaacg cccaaggtct
     1201 agtcagttct cgtttaatgc tattgaaata ctttgacaaa aatgggttgg tattttttac
     1261 taattaccat agtcctaagg cacaagcatt acaagaacat ccacaagcca cccttctctt
     1321 ttggtggtcg gaattcgaac gtcaagtgcg tatcgatggt ttagtggaaa aattagatag
     1381 tgagcattcc gatgcttatt ttcaaaaacg cgaacgtgaa tcgaatattg cggctatgct
     1441 ctcgaagcaa agcactccgc taaacgataa aaaaagtttt atcgatttat ttgagaaaga
     1501 acgcaacaaa tcgtcggacg atgcattgat acgacctcct ttttggggag ggtatcttct
     1561 aaagcccgta tactatgaat tttggcaagg tggccctcat cgattacatg ataggcttgt
     1621 ttatcgaaaa ataaaggaag attgggaaat aactagactt tatccataat ttttgcggga
     1681 ataacaatat ctgctaaagc ttcactcaaa aaattttgca gtattttagt aacgatttca
     1741 ggctgttcca cggaaggaac atgacctgaa tctgcaataa tttttaattt agcatgggga
     1801 atatagcgtg ccatagtctc tgcttcgtgt ataggccttg gataatcgtt ttcgccaacc
     1861 ataatgagta cgggcatttt taataaacct aatttatcta aaatggatct acgcgaaaaa
     1921 atcgtatggc ctatttcaat taaaccttct atttgctcga caggtaattg ctttaatttt
     1981 gttttaaaat cttttataag ttccggcttt gtatgaaaag tactcggtgt gaaaaaaaat
     2041 ggtaaaattt tttcaataat ttcatcggat aagtgctgtt tatgttccca ggagtggagt
     2101 atctccgaat acatgagctt tctttcaata ggctcttcac cgacaaaagt gtccatcata
     2161 attaatgctt taaccgcgtt tgcatgattt aaactcagtt gagctcccca cattccactg
     2221 atcgattgcc cgatcacagc aaactcaggc aaatttaact tatccatgag ataataaaaa
     2281 tctttactta acagctcgat gctgtaagga cgttgaggaa cactttcaga agcaccatga
     2341 ctccaaagat cgggaacgat acaacggtag tgttgagaaa gtatttctat ttgtggctgc
     2401 cacattttcc tatcccacaa aaaaccatga ccaaataata gtggaaaacc ggatcccaca
     2461 tcttggtacc atagtcgtct atgtcgatgg atgataaaag gcataatcac taatttaaag
     2521 aaagatactt aagtttatcg cgcgatcccc agtagatcaa atacctcgaa atattgttaa
     2581 ttatataagt tgatttactt aatttcagaa catcggggat attcatggat ttgctaaaaa
     2641 atgaagttat taattttttt aaaataatga caggtctaaa atactaagat ttaacttgtt
     2701 aaaaataata aattgtcatg ccgataaaaa ctataaagaa tttataacct aaataaaagg
     2761 cagtgattaa tttctgactt acttccttct tatggatcga gaagaattga gtttacctaa
     2821 atattctgta tacaatataa aaaaaggaag tttgtaaaaa agaaattttt aatggaaata
     2881 gcgttttttt atccctacga caaaatttac agtccattca aacacaaaac tgtattcatt
     2941 atatctttta ttcttattct atttacgctt ta
//
LOCUS       DASTIB010000280         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1306077, whole genome shotgun sequence.
ACCESSION   DASTIB010000280 DASTIB010000000
VERSION     DASTIB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306077"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFP93_04850"
     CDS             <1..1155
                     /locus_tag="VFP93_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843965.1"
                     /translation="KMNFLSQLNDIRNAHGPDDPTCYPGAITRSADMGSFHEVAQLPI
                     TTKEWLSNYFKTQALAALDQEFAKLTSFEEKQDLTHSLTQLTEGSVKTIIQDPANYPQ
                     EWLTLRKRFIQSLGTAQENLEKIFLEDTTPITQADLIYIEQCLLDIGAGDRGQAIATF
                     FNRNTDHIPSQEEIEQANPFKTHENAYALSKMMLDIILHLVPSYTKSMRQLKALTEFL
                     QQKVPALLQGQPLSEVIDDMRLAPESQAAVFQAIDANWKFAPAVINPFSAKIEAARKQ
                     IELKANQPQFQMVFKRQLDNLLRKSELFLNHYPMIKEFVENANPKLDATDAIRIAEEL
                     ANYFAVEETFNQVEFQKQFSEAYVNKNAALFETLSLTLTKPADQTPRASL"
     gene            1314..>2066
                     /locus_tag="VFP93_04855"
     CDS             1314..>2066
                     /locus_tag="VFP93_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9843966.1"
                     /translation="MKRGPELDLNSISKREKTENIPQEQLNKELLAAVKAGDLQRVES
                     LLQTGANPNHCAYIQRIFIREIINGELIKIPPDPSASTKSVPLLAIACAKNYLPIVKT
                     LIQFGADKKITIESTPALHYVMRMGQIALAEVLIESGFDLEETDHDRTALHHAIELNN
                     HVLVKKLLEHGANTEALYDGQTALHVAVRRNFVEVVKLLCENHANLEALYNEQTPLHS
                     AIQYGIEDSAKILIDANCNLESIYKNATPLHNA"
BASE COUNT          711 a          383 c          395 g          577 t
ORIGIN      
        1 aaaatgaatt tcttaagtca attaaatgat attcgaaacg cgcatggtcc tgatgatcct
       61 acatgttatc ctggcgcaat tacgcgtagt gcggatatgg gttcttttca tgaggtggca
      121 caattaccca ttactaccaa agagtggtta tccaattact ttaaaaccca agctttagca
      181 gcattagatc aagaatttgc aaagctcaca agttttgaag aaaaacaaga tttgacccac
      241 tctttaacac aattaacaga gggatctgtt aaaacgatta tacaagatcc tgcaaattat
      301 ccacaggaat ggttaacatt aagaaagcgt tttatccagt cgttaggaac tgcccaagag
      361 aatttagaaa aaatcttttt agaagataca actccgatta cccaggccga cttaatttac
      421 attgaacaat gtttattgga tattggcgca ggggataggg gacaagcaat tgctacattt
      481 tttaatcgaa ataccgatca tattccaagt caggaggaga tcgagcaagc gaatccattt
      541 aaaacgcacg aaaacgccta tgcactcagt aaaatgatgt tagatattat tttacatttg
      601 gtaccaagct ataccaaaag tatgcgtcaa ttaaaggcac taacagaatt cttgcaacaa
      661 aaagtacctg ctttactgca aggacagccg ttaagtgaag tgattgatga catgcgtctt
      721 gcacctgagt cacaggctgc tgttttccag gctattgatg ctaattggaa atttgcacca
      781 gcagtaatca atcctttttc ggcgaaaatt gaagctgcta gaaaacaaat agaattaaaa
      841 gcaaatcaac cccaatttca aatggtattt aagcgccaat tagataactt attgcgaaaa
      901 agcgaactat tccttaatca ttatccgatg ataaaagaat ttgttgaaaa cgcgaatcca
      961 aaattggatg ctacggacgc gattcgtatt gcggaagagt tggcgaatta ttttgcggtc
     1021 gaagaaactt ttaaccaagt tgaattccaa aagcaattta gtgaagcata cgttaataaa
     1081 aatgccgcgt tgtttgaaac cttgtctctc acattgacga aacctgcaga tcaaacaccg
     1141 cgtgcttcgc tttaaaattt gtcactagcg ctaaggggat catttaggga tccccacctt
     1201 gcgttagaaa atttgggaac actgcggtat ctttctttaa taccaaaaaa atccagaagt
     1261 ttttgtcgag acacctgctc aatgcagtta aagaaattat tagaggtatt tttatgaaac
     1321 gtggacctga acttgatttg aattcaatct caaagcgtga aaaaacagaa aatattcccc
     1381 aggaacaatt aaataaagag ctcttggcag cggtgaaagc tggtgattta caaagggtag
     1441 aatctctttt gcagacaggt gctaacccta atcactgtgc atacatacaa agaatattta
     1501 tacgcgagat aatcaatggc gaacttataa aaataccacc cgacccaagt gcatcgacga
     1561 agtcggtacc gctattagca atagcatgcg ccaaaaacta tcttccaata gtaaaaacac
     1621 ttattcagtt tggagctgac aaaaagataa ctattgaatc aactccggca ttgcactatg
     1681 tcatgcgaat ggggcaaatt gcattagctg aagttcttat tgaaagtggt tttgatcttg
     1741 aagaaacgga tcatgataga actgcgttac atcatgccat agaattgaat aatcatgtac
     1801 tcgtgaaaaa attacttgag catggtgcaa ataccgaagc actttacgat ggtcaaactg
     1861 ctttacatgt tgcggttcga cgtaattttg ttgaagttgt taaattgctt tgtgaaaatc
     1921 atgcaaactt agaagcgtta tataatgagc agacaccgct gcatagtgct atccaatacg
     1981 gtattgaaga ttctgcaaaa atattgatcg acgctaattg taatttagaa tctatatata
     2041 agaatgctac accgctacat aacgca
//
LOCUS       DASTIB010000281         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_274711, whole genome shotgun sequence.
ACCESSION   DASTIB010000281 DASTIB010000000
VERSION     DASTIB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274711"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..833
                     /locus_tag="VFP93_04860"
     CDS             129..833
                     /locus_tag="VFP93_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843967.1"
                     /translation="MNTYKYLLHHLYYYKSDICQFILKPTHDAIAIEAGQYVLFHLPC
                     GINAPFSIAGFQHHHLEFHVRINEANIFIQEFLTHLQQVESIEISGPYGKCVLPQNKH
                     IPLIFAAGGTGIAPHKALIDEALKSNYASIRLYWGIKTLNDLYLKSWLQRCESFENFQ
                     ATVVLSEVPEDGLFERGLVHHYVLQEPKLSQGVLYASGPFAMIKALQLGSQQLGLKNI
                     HVKSDMLNFNSDEPTS"
     gene            complement(830..1579)
                     /locus_tag="VFP93_04865"
     CDS             complement(830..1579)
                     /locus_tag="VFP93_04865"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET9843968.1"
                     /translation="MTGWISGRVVSQHKWTDSLYSLYVQTDPIPFIAGQFCRIALDID
                     DKRIARPYSFVNSPNDSIHEFYYNHVPNGLLTEQLIKLSTDDTLWVASKGAGLFTLKQ
                     VQDAKTLWLFASGTALGVFLSILKTAEPWIRFQRIVLMHAVRFTQELTHQALIEELQA
                     THPEQLLYFPCVSRETMAQGFHGRITHALQTGALEDKAGLLLNPATSSAMICGNPAMV
                     KDTFELLKEKGLSVSHPKTPGQITIENYWKD"
     gene            complement(1580..>2349)
                     /locus_tag="VFP93_04870"
     CDS             complement(1580..>2349)
                     /locus_tag="VFP93_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843969.1"
                     /translation="SELAIGLARAEMDRQSNQLDLSLTTLEQLQKKYPKNKEILRLIK
                     ETALLLRDWNTLIKFLPTLKRHEILPAEEFRQLQTHVYLHWLNESAVSTSPDNFQESW
                     QSLPKDVQYSPEIIQVYIQKMMDRLDHEELANIIRITLKKQWNEQLILLFGQLKTNQS
                     AKRLSIAEGWLKTHAQSANLLLTCGRLAAQNDLFGKAERYLEACVHLTNNPEAYATLG
                     LLYEKMGKTDLSADCFRKGMLETFSQPTNLLMHHQDL"
BASE COUNT          691 a          439 c          475 g          744 t
ORIGIN      
        1 agatgggatg ggatgccacg acaaaatggt atggcgaaac cgagcgtacc tggggtattc
       61 cgatagccca agatcccaag attgctgcga ggattgatag catttggcaa gaattgggat
      121 tataaatttt gaatacctat aaatatcttt tgcaccattt gtattattat aaaagtgata
      181 tttgtcaatt tatactcaag ccaacacacg atgcaatagc aatagaagct ggtcaatatg
      241 tgctttttca tttaccctgc ggtatcaacg ctcctttttc catcgcaggc tttcaacatc
      301 accatttaga atttcatgta cgtatcaatg aagcaaatat ttttatccaa gaatttttaa
      361 cacaccttca acaagttgaa agtattgaaa taagtggacc ctatggaaag tgtgttcttc
      421 cccaaaataa gcatattcca cttatttttg cggcaggcgg taccggaatt gcaccgcaca
      481 aagctttaat tgatgaagct ttaaaatcaa actatgcatc gattcgtttg tattggggca
      541 tcaaaaccct taacgattta tatttgaaat cttggttaca aagatgtgaa agctttgaaa
      601 actttcaagc aacggttgtt ttgtctgaag taccagaaga tggtttattt gaaaggggtc
      661 tggtacatca ttatgtttta caagagccaa agctgtccca gggtgtgctc tatgcaagtg
      721 ggccgtttgc tatgataaag gcactccaat taggatccca acaactgggt ttaaaaaata
      781 ttcatgtaaa atcggatatg ttgaacttta attcggacga gcccaccagt taatctttcc
      841 aataattttc aatggtaatt tgaccgggag ttttaggatg cgaaacgctt aacccttttt
      901 cttttaataa ttcaaatgta tctttcacca tagcagggtt gccacaaatc atagcactgg
      961 aagttgctgg atttagtaat aaaccggctt tatcttccaa cgcacctgtt tgcaaagcat
     1021 gtgtgatccg gccatgaaag ccttgtgcca ttgtttctct acttacacaa gggaaatata
     1081 ataattgctc aggatgggtt gcttgtaatt cttctattaa agcttgatgg gttagttctt
     1141 gcgtaaaacg cacagcgtgc attaaaacaa tgcgttgaaa tcgtatccaa ggttcagccg
     1201 tttttaaaat agagagaaac accccaaggg cggtacccga tgcaaacagc cataacgttt
     1261 tggcgtcttg tacttgtttt agcgtaaaaa gacctgctcc tttagatgca acccataggg
     1321 tgtcatcggt ggatagctta attaattgtt cggttaacag gccattggga acatggttat
     1381 aataaaactc atggatggaa tcattaggcg agttaacaaa agagtaggga cgcgcgatgc
     1441 gtttgtcatc gatatccaat gcgatacgac aaaattgtcc cgcaataaat gggataggat
     1501 ccgtttgcac ataaagagaa taaagagagt cggtccactt gtgctgtgaa accacacgtc
     1561 ctgagatcca gcctgtcatt tataaatctt ggtgatgcat taataagtta gttggttgag
     1621 aaaaggtttc taacatccct tttctaaaac agtcagcgct caaatcggtt ttacccattt
     1681 tttcataaag aagccctaat gttgcatacg cttcagggtt attcgttaaa tggacacacg
     1741 cctcgagata acgttctgct ttgccaaaaa gatcattttg cgctgcaaga cgaccgcatg
     1801 tcaagagtaa gtttgcactt tgcgcatggg tttttaacca accttctgca atacttaaac
     1861 gtttagcgga ttggtttgtt tttaattgcc caaacagcag tataagttgc tcattccatt
     1921 gtttttttag agtaattcga atgatattcg ctaattcttc atgatctaac ctgtccatca
     1981 ttttttgaat atatacctgt ataatctctg gagaatattg cacgtctttg ggtaaggatt
     2041 gccaactttc ttgaaaatta tcgggagaag ttgacactgc agattcgttt aaccaatgta
     2101 aataaacatg cgtttgtaat tgtcgaaatt cttccgcagg caaaatttca tggcgtttta
     2161 gggtgggtaa aaattttatt aacgtgttcc aatcccgtag taaaagtgca gtttctttta
     2221 ttagccgtaa aatttcttta ttttttggat attttttttg caactgttcc agggtggtta
     2281 aactcagatc gagttggtta gattgacgat ccatttctgc tcgcgcaagt ccaatggcaa
     2341 gctcagact
//
LOCUS       DASTIB010000282         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_915635, whole genome shotgun sequence.
ACCESSION   DASTIB010000282 DASTIB010000000
VERSION     DASTIB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915635"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="hslV"
                     /locus_tag="VFP93_04875"
     CDS             complement(<1..478)
                     /gene="hslV"
                     /locus_tag="VFP93_04875"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET9843970.1"
                     /translation="MDQYRGTTIVVVRRNGQVALGGDGQVSLGNTILKGNARKVRRLY
                     QNRVLAGFAGGTADAFTLFERFERKLEMHHGQLVRAAVELAKDWRTDRILRRLEALLA
                     VADKTSSLILTGNGDVIEPEKGLIAIGSGGPFAQAAATALLEETDLNAQTIVEKALS"
     gene            complement(510..1106)
                     /locus_tag="VFP93_04880"
     CDS             complement(510..1106)
                     /locus_tag="VFP93_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET9843971.1"
                     /translation="MTRDYAKKRGRGRNYSGSQRAKYSVSALIPGWVWLLIGIISGIC
                     LACFISWKWSQKIESRPPAPPIIISDTNTLKEEAEENKPRFDFYTLLPNLKVDLPDVA
                     TPPSARTQTNTKQQDASLAYIIQAGSFKTQDQADKRKATLALQGIESQIQTVNIRPEE
                     TWYRVYIGPFKTKEEAQNVQQNLEQNQALNSLILKIRV"
     gene            complement(1190..>3008)
                     /gene="priA"
                     /locus_tag="VFP93_04885"
     CDS             complement(1190..>3008)
                     /gene="priA"
                     /locus_tag="VFP93_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET9843972.1"
                     /translation="SQLKNAFQLLDNMPIFPSNYWLFLNKAHRYYHHPLGEILITGMP
                     LALRKGEPLAHSMPLTKNAFVSGENQQPSFNLNPEQKNTLDHIRKCIGRYQTFLLEGV
                     TGSGKTEIYRQLIQTCQKQNQSVLVLLPEIALTPQTLARFQQGLSGNLLIYHSKLSQK
                     TRVSVWAHIKNNPNSVVIGTRSALFLPFEKLGAIIVDEEHDPSFKQQEGFKYSARDLA
                     VLRAQIDQCPIVLGSATPSFESIFNANVGKYKKVVLSTRANQQILPKIQIIDIRHKQL
                     KDGLSNHVIESIRSHLHKQYQVLIFLNRRGYAPVWMCYTCGHIAQCERCDVKLTFHKH
                     KSTLICHHCNKKYSLIEKCVHCQNSMLEVGVGTERLEIGLQELFPEIPIVRIDSDTTR
                     KKGVLSNKLQQIHESTAQILIGTQLLAKGHHFPNVTLVAIVDVDAGLFSNDFRSAERM
                     GQLITQVAGRAGRESLSGEVLLQTTQPEHPYLNVLIEQGYGAFASALLQERKQTLLPP
                     YCSLAMVRASAQKNVLPEQFLTKLKHALTAVGTIDVWGPFPSSISRKQGYYRYHLMLQ
                     AATRNQLHRILAKLNGIILTIPESKKVRWSLDVDPIEMG"
BASE COUNT          854 a          605 c          593 g          956 t
ORIGIN      
        1 tagataatgc tttttccact atggtttgtg cgtttaagtc tgtttcttct aggagagcgg
       61 ttgcagctgc ttgggcaaat ggaccgcccg aaccaatcgc aattaaacct ttttccggtt
      121 caataacatc gccatttcca gttaaaataa gtgaggaagt tttatccgca actgctagta
      181 aagcttctaa tcttcgcaag attcgatcgg tacgccaatc tttcgctaat tcaactgcag
      241 ccctcacgag ttggccatga tgcatttcca gcttgcgttc gaaacgctca aaaagcgtaa
      301 aggcatctgc ggtcccaccc gcaaaacccg ctaaaacacg attttgatag agcctgcgta
      361 cttttcgggc attgccttta aggatagtat tgcccaagga aacttggcca tcaccaccta
      421 aggcaacttg accattgcgt ctgacaacca caatcgtagt tccacgatat tgatccaaag
      481 atcctccaac aaattttaaa aattaaacat catacgcgaa tttttaagat caggctattt
      541 agcgcctgat tttgttctaa attttgctga acattttgtg cttcttcttt ggttttaaaa
      601 ggtccgatat aaacacgata ccaggtttct tctggacgta tgttaacggt ttgaatttga
      661 gattcgattc cttggagtgc caacgttgct ttacgtttgt cagcctgatc ttgggtttta
      721 aacgaacctg cctgaatgat atatgctaaa gaagcatcct gttgcttggt atttgtttgc
      781 gtgcgtgcgg aaggcggagt tgccacatca ggcaaatcta cttttaagtt tggtaacaaa
      841 gtataaaagt caaagcgtgg tttattttct tccgcttctt cttttaaagt attagtatcg
      901 ctaataataa taggtggagc aggaggacgg ctttcaattt tttgactcca tttccaagaa
      961 ataaagcaag ctaaacaaat tccagatata ataccaatta ataaccaaac ccaccctgga
     1021 attagtgccg aaacggagta ttttgccctt tgcgagcccg aataattacg accgcgtccg
     1081 cgttttttag cataatctct tgtcattttg gtttgttttc ttaatattta gaataaagtg
     1141 gcgaaaaacc atgccgtatg gcaaagtgtc gtacagtgca aaatgtattc taacccattt
     1201 caatcggatc cacatctaaa gaccatcgca cctttttgct ttcaggaata gttaggatga
     1261 tcccatttag cttagctaaa atacgatgta attgatttcg agtagcggct tgtaacatca
     1321 aatgatagcg ataataacct tgttttcttg aaattgaaga tggaaaaggc ccccaaacat
     1381 caatagtgcc taccgcagtc aaagcatgtt tcaatttcgt taaaaattgt tctggtaaaa
     1441 catttttttg cgcgctcgcg cgtaccatag ctaagctaca ataaggtggc agcaaagttt
     1501 gttttctttc ttgcagaagt gcactggcaa aagcgccata tccttgttca attaaaacgt
     1561 ttaaataagg atgttcaggc tgtgtcgttt gtaataaaac ttctcctgag agagattctc
     1621 gaccggctct acctgctacc tgcgtaatta attgccccat gcgttcagca gatcgaaaat
     1681 cgttactgaa caaccctgca tctacgtcga caatagcaac caaagtcaca ttcggaaagt
     1741 gatgaccttt tgctaatagt tgcgtaccaa ttaatatttg tgcagtggac tcgtgaattt
     1801 gctgaagctt attcgacaat acgccttttt ttcttgtcgt gtctgagtcg atgcgaacga
     1861 taggaatttc aggaaaaagc tcttgtaagc ctatctctaa tcgttcggtg ccaacaccaa
     1921 cctcaagcat gctattttgg caatgcacac acttttcgat taagctgtat tttttattgc
     1981 aatgatgaca aattaacgta cttttatgtt tatggaaggt aagttttaca tcacatcttt
     2041 cacattgtgc aatatgaccg caggtataac acatccaaac gggcgcgtaa ccgcgtcgat
     2101 tgaggaaaat taacacttgg tattgcttat gtaaatgcga acgaatggat tctataacat
     2161 gattacttaa accatctttt aactgtttat gtcgtatgtc gattatttgt atttttggta
     2221 agatttgttg attggcacgc gtagacaata ctactttttt atatttgcca acgttagcgt
     2281 taaaaatgga ttcaaacgat ggtgtagccg aacccagtac aatcggacat tgatcgattt
     2341 gggcgcgcag cactgccaaa tctcgcgcag aatatttaaa accttcctgt tgtttgaatg
     2401 aaggatcgtg ttcttcatcg acaataattg cacctaactt ttcaaaaggt aggaacaatg
     2461 cagaccgtgt gccaatcact actgaattcg gattattttt aatatgtgcc cataccgaaa
     2521 ccctggtttt ttggcttaac ttggaatgat aaattagcaa gtttccgctt aaaccctgtt
     2581 gaaatcgtgc taaggtttgc ggcgtgagtg caatttctgg aagcaaaacc aataccgatt
     2641 ggttttgctt ctggcaagtt tggatcaatt gccggtaaat ttctgttttt ccacttcctg
     2701 ttacaccttc caacaaaaaa gtttgatacc gtcctataca ttttcgaata tgatctaaag
     2761 tatttttttg ctctggattc agattaaaag agggttgttg attttcgcca ctcacaaaag
     2821 cattcttggt aaggggcatg gaatgcgcta gaggctctcc ttttctcagt gcaagtggca
     2881 tgcccgtaat taaaatttca cctaagggat ggtgatagta acgatgtgct ttgtttagaa
     2941 aaagccagta attggatgga aaaattggca tgttatctaa tagttgaaat gcatttttta
     3001 attgcgac
//
LOCUS       DASTIB010000283         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_356113, whole genome shotgun sequence.
ACCESSION   DASTIB010000283 DASTIB010000000
VERSION     DASTIB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356113"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..147)
                     /locus_tag="VFP93_04890"
     CDS             complement(4..147)
                     /locus_tag="VFP93_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843973.1"
                     /translation="MVSLNSAMTVPRLPEFSETTREIERLFRLTSENQYPLQEWIADI
                     KRH"
     gene            complement(239..673)
                     /locus_tag="VFP93_04895"
     CDS             complement(239..673)
                     /locus_tag="VFP93_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843974.1"
                     /translation="MLLTTLFLHALRFGPHIQLNLQKIPIVGFFGPLIISLIILTFIP
                     VGYAYQSTISLLLSLSVLYRIDTPDTKTPSSAFAKFITLGFVALGYFLLTNTPTPFVI
                     NCFLYIVLLEIARRYILIDTSVTLTRLQSILKFREDSNSRSA"
     gene            1339..>2965
                     /locus_tag="VFP93_04900"
     CDS             1339..>2965
                     /locus_tag="VFP93_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9843975.1"
                     /translation="MKYDYIIITHLPSFYKVNLYNKLAQRLRICVIFLGRTSCIRTED
                     FILEDFQFDAVYLSNGNFEQRNKLQTVARLVKNLKQHSFKKLLVSGWDQIEFWIALLF
                     SKKEKNCLSLESGFESQRYGLKKLLKQIFLAKIHQVFATGLPQIELLKSLHYKGAMLR
                     TLGVGIFHTNHSKMPTSFKRKFIYVGRLAPEKNLPTLIEAFKALPEFNLTIVGYGSYR
                     VPKLPNIHLLGHVPQAQMGKLYTEYDVLILPSLHEPWGLVVEEALAYGLPVIASERVG
                     SKEIIEKYKCGLLFDPNKVESLIECIQFIAKADMYSAYQANTQSNWKQIRDAIQVNAY
                     MEGLWATSCKPSSDAVFSIEQPANTKLTLPIRLGQKAKSTDILSPKTLQLKILLIHNY
                     YKTAYKGGEDVVFEREVEALKNILGEENVFVYTVHNNDVKLWHLPFTIWFNYTHYNNV
                     LKLCRTHNIQLVHVHNFFPALTFAPFLAAKRSGCKVVHTLHNYRFWCLNGSLFYKNAI
                     CETCTHKKIALQGIVRGCYRGSKMQSLLTALSFA"
BASE COUNT          994 a          486 c          578 g          907 t
ORIGIN      
        1 catttagtgt cgtttaatat ccgcgatcca ttcttgtaaa ggatactggt tctcggatgt
       61 taagcgaaac aaacgctcga tttctcgcgt tgtttcgcta aactccggga gacggggtac
      121 cgtcatcgcg gaatttaacg acaccatgcg agcttgcgag catttagtgt cgtttaatat
      181 ccgcgatcca ttcttggtcg gacttgattc cggatgtcaa gcgaaactga acgctcgatt
      241 atgcggatcg actgttactg tcttcgcgga atttcaagat actttgcaag cgtgtgagcg
      301 ttaccgatgt atcgattagt atgtaacgcc tagctatttc tagcaatacg atatacaaaa
      361 agcaatttat cacaaaaggc gtaggggtat ttgttaacaa aaaataacct aaagcgacaa
      421 atcccaaggt tatgaacttt gcgaaagcag acgaaggtgt ttttgtatca ggtgtatcta
      481 ttctataaag cacagaaagg cttaataaaa ggctgattgt ggattgataa gcatatccca
      541 cgggtatgaa tgttaaaata attaaactga ttatcagtgg accaaaaaat ccaacaatgg
      601 gaattttttg taaatttagt tgaatgtggg gaccaaagcg tagcgcatgt aaaaaaagcg
      661 ttgttaaaag caacccgatt actaatacgg caaaaaaaca tcactgataa aatgcatgcc
      721 tacgtaaaca cgcgatatgc aaattaaggt aacatagagc attgccagat aaaatacgat
      781 tttattttta caatgataaa ttaacaagag cgctagtgat atgcacattt gcgcaccgcc
      841 acttggtaat ccatagagtg aatccgtttg tactaaacct aaatttttat cgagccataa
      901 tggacggaat gtaaaataaa agtttaaaaa ataattagag gctgtactga gtgctaataa
      961 taaaaagtag cgtttgccga taagaggatt tccaaaaata tataaaagtg taccaacgag
     1021 acaataaaag ccttgactcc caatgatgca aaaatataaa aaaaggatct aaaaatgcaa
     1081 ttctatacga tagcagtgct tgtagtaaat ttaattcgta aggaatataa ctgaacaatg
     1141 aggtgtccta gaataactgt agctttagat cttaaaaatc taattttatt gtgttataaa
     1201 gcgtgtcatc ttaaattact aaacttgaaa taattttgca gtattttcgc aagagtttgt
     1261 gatctgaata atacttttct atcgtttccc atagaatctt tatggaagtg aatttatgaa
     1321 tgtgggctgc aagaagcaat gaaatacgat tacattatca taacgcattt acctagtttt
     1381 tataaagtaa acctttataa taagcttgca caaagattgc gtatatgtgt tatttttctg
     1441 ggcagaactt cttgcattcg caccgaagat tttattttag aagattttca atttgatgca
     1501 gtatatttat caaacggtaa ttttgaacaa agaaacaaat tacaaacggt agcgcgatta
     1561 gtaaaaaact taaagcaaca tagttttaag aagctgcttg ttagtggttg ggatcaaatt
     1621 gaattttgga tagctttact ttttagtaaa aaagagaaaa attgcctatc tttagaatcc
     1681 ggctttgaaa gccagcgcta tgggttaaaa aaattattaa agcaaatttt tttggcgaaa
     1741 atacatcaag tatttgcaac aggtttacca caaatcgagc tgttaaaaag tttgcactat
     1801 aaaggcgcga tgcttcgtac tttaggcgta ggtatatttc acacaaatca ttcgaaaatg
     1861 ccaacctcgt ttaaaagaaa atttatctat gtggggcgat tagccccaga aaagaattta
     1921 cctacattga tagaagcttt caaagcactt cctgaattta acttaactat tgtaggatac
     1981 ggttcttatc gtgtgccaaa gcttccgaat attcacttat tgggtcatgt gcctcaagcg
     2041 caaatgggta aactttatac cgaatatgat gtattgattt taccttcctt acatgagcct
     2101 tgggggttgg tggtagaaga agctttagcc tatggattgc ccgtcattgc ctcagaacgg
     2161 gtgggttcaa aggaaattat tgaaaaatat aaatgtgggt tattatttga tccaaacaaa
     2221 gtggaatcct taattgagtg tattcaattt atagcaaaag ctgatatgta ttctgcgtat
     2281 caagcgaata cacagagcaa ttggaaacag attcgagatg cgattcaagt gaatgcctat
     2341 atggaagggc tttgggctac ctcgtgtaaa ccttcatccg atgcggtgtt ttctatagag
     2401 caaccggcga ataccaagct gacattaccc atacgtcttg ggcaaaaagc aaaaagtaca
     2461 gatatccttt cgccaaaaac tttacaattg aaaatcttgt tgattcataa ttattataaa
     2521 actgcttata aaggtggtga agatgttgtt tttgagcgcg aggttgaggc gttaaaaaat
     2581 attttgggcg aagaaaatgt tttcgtatat accgtacata ataatgatgt aaagctttgg
     2641 catttacctt ttaccatttg gtttaattat acacactata acaatgtatt aaagttatgc
     2701 cgtacgcata acattcaatt ggtccatgtc cataattttt tcccagcact tacctttgcg
     2761 ccttttcttg ctgcaaaaag atccggttgc aaagtggtgc atactttaca caattatcga
     2821 ttctggtgct taaatggaag tttattttat aaaaacgcta tatgtgaaac gtgtacccat
     2881 aaaaaaattg cgctgcaagg aatagtgcga ggttgttaca gaggctcgaa aatgcaaagc
     2941 ctgttaaccg cactaagctt tgctt
//
LOCUS       DASTIB010000284         6575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_8952, whole genome shotgun sequence.
ACCESSION   DASTIB010000284 DASTIB010000000
VERSION     DASTIB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8952"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFP93_04905"
     CDS             <1..767
                     /locus_tag="VFP93_04905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9843976.1"
                     /translation="ETDDDSTIIDIIDAGADDFLKKPVSLGLLKAKLKSMQRLMAMRQ
                     NLIDYGQQLRTVNDNLLSSTQLLNELSLKDPLTLIGNRRAFQEIFDKALRSAIRNGEP
                     LSMVMIDIDYFKLYNDTLGHQTGDFCLKEVAQLFANAMHRPKDFIARFGGEEFAVLLP
                     ETDLKGACQVADRLRIILEDKKIEHPASTFRYVTASFGVACTIPFRGFRSEDLLRIAD
                     LALYQAKQEGRNCVVHCAETVESLKFHAEPPLQKYA"
     gene            809..1636
                     /locus_tag="VFP93_04910"
     CDS             809..1636
                     /locus_tag="VFP93_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005493350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HET9843977.1"
                     /translation="MSTQVGIISKLTAEHTTPINYFLHLGADPISLNGLLGKKLRLRF
                     TGKIHCNHCARPIKKTYRGYCFPCSQKLASCDLCIVKPELCHFHLGTCRDPRWASQHC
                     MIPHYIYLANTSELKVGITRKNHIPNRWLEQGATSALPIIEVKTRQQSGFVEEIFKQF
                     VKDKTNWRQLLRCENENVDLRALASILLEKADTGLQKVLEYYGKENITLLKDPFLFQF
                     SYPVIQYPRKLKSLALDKEKEISDTLLGLKGQYFIFDEGVLNIRKHTGFEIEFEAIN"
     gene            complement(1629..3164)
                     /locus_tag="VFP93_04915"
     CDS             complement(1629..3164)
                     /locus_tag="VFP93_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET9843978.1"
                     /translation="MKSKSAKSSRPLATSNEWNFELLTTFDQELGKLAKTFGLDTYPN
                     QIEVISAEQMMDAYSSTGMPLGYNHWSFGKQFLTVEKNYKRGHMGLAYELVINSDPCI
                     AYLMEENTLTMQVLVLAHACYGHNSFFKNNYLFKTWTSPDSIVDYLVFARNYIAECEE
                     RYGMDEVETILDACHALMTQGVDRYKRPNKISLREEKIRQAKRADYMQSLVNELWRTL
                     PQRKKETKDVKEEIYPAEPQENILYFIEKNAPLLEPWQREIVRIVRKMAQYFYPQRQT
                     KVMNEGWATFWHYTLLNQLYDEGLVNDDFMMEFLHSHTNVIYQPTYESPYYNGLNPYT
                     LGFSIYSDLRRLCENPTEEDKRWLPDFAGQPWIQTLDFAMKNFKDESFIAQFLSPKVV
                     RDLKLFCILDDDQKEDLEVSFIHNEEGYHYVRRLLSENYNIGNIDPDIQVYKVDKRGD
                     RSLTLRYTQNKRRPLSSEDTEEVLRHVHTLWGFPVKLETVQHDGTIELVYQCPPVSEA
                     NVS"
     gene            complement(3172..4446)
                     /locus_tag="VFP93_04920"
     CDS             complement(3172..4446)
                     /locus_tag="VFP93_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HET9843979.1"
                     /translation="MSRFIDRRPNGRHKSAVNRQRFMRRYKRQIQEAVRHAITGRSIK
                     EVDESGEKIYIPAKDINEPQFRYGDGGERHLILPGNKEFVPGDLLKRPPRNSGNGRGG
                     KASNGAEGMDDFVFEISKEEFLELFFEDLALPNLIKTQIAKIPAFKMIRAGFTHEGNP
                     SNINIVRSLKGAVARKVALSAPSKEKLEQLELELANLLAIHPESPKVTELKEEIELLK
                     KRLHAIPFIDPFDLRYNNRVRQPKPTTQAVMFCLMDVSGSMDEAKKDIAKRFFILLYL
                     FLSRNYENVEVVFIRHHTSAKEVDEEEFFYSRETGGTVVSSALELMKKIMMSRYPTTD
                     WNIYAAQASDGDNWNNDSPRCRDLLLYDILPHVQYFAYVEIMPRHHQSLWEAYLTVKE
                     SSPQFAMQHIEEIKDIYPVFRELFKRQESITS"
     gene            complement(4558..6483)
                     /locus_tag="VFP93_04925"
     CDS             complement(4558..6483)
                     /locus_tag="VFP93_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HET9843980.1"
                     /translation="MDVLSNFKDRYYGKTQEEELTLQEYLERCKHDRMAYANAAERLL
                     HVIGEPRLVDTSKDPRLSRIFSNKVIKLYDSFHEFYGMEEPIDQIVSFLKHAAQGLEE
                     KKQILYLLGPVGGGKSSLAERLKSLIEHVPFYALKGSPIFESPLGLFDIIEDGPILEE
                     EYGVPRRYVEAIMSPWAVKRLNEYNGDINQFKVVKLWPSRLRQIAIAKTEPGDENNQD
                     ISSLVGKVDIRKLEKYAQDDADAYSFSGGLCKANRGMLEFVEMFKAPIKVLHPLLTAT
                     QEGNYNGTEGSASMPFDGIIMAHSNESEWQTFRNNKHNEAFLDRVYIVKVPYCLRVTE
                     EIKIYDKLLQNSSLRDAPCAPNTLKMMAQFSVLSRLKEPENSSIFSKMRVYDGENLKD
                     TDPKAKSYQEYRDFAGIDEGMTGLSTRFAFKILSKVFNFDNTEVAANPVHLLYVIEQQ
                     IEREQFPPELHERYMSYIKGFLSPRYVEFIGKEIQTAYLESYSEYGQNIFDRYVTYAD
                     FWIQDQEYRDPDTGEILERRSLNDELEKIEKPAGISNPKDFRNEIVNFVLRARANNRG
                     KNPSWTSYEKLRAVIEKKMFSNTEDLLPVISFNTKASADEKRKHQDFVDRMVDKGYTP
                     KQVRLLCEWYLRVRKSS"
BASE COUNT         1932 a         1288 c         1238 g         2117 t
ORIGIN      
        1 gtgaaacgga tgacgattcc actatcatag acattattga tgcgggtgcc gatgactttt
       61 taaaaaagcc cgtatcgtta ggattattaa aagcaaaatt aaagtctatg caacgattga
      121 tggcaatgcg tcaaaatttg attgattacg gtcagcaatt gcgtaccgta aatgataatt
      181 tgctttcttc tacacaattg ttaaatgaat tatcacttaa agatcctctt acgttaattg
      241 gtaatcgccg tgcattccaa gaaatttttg ataaagcgtt acgaagtgca attcgcaatg
      301 gcgagccttt gagcatggtc atgattgata ttgattattt taaactttat aacgatacct
      361 taggccatca aacgggagat ttttgtttaa aagaagtagc acaattattc gcaaatgcta
      421 tgcaccgacc caaagatttt atcgcgcggt ttggtgggga ggaatttgca gttttattgc
      481 cagagactga tttaaaaggt gcctgtcaag tggcagatcg tcttcggatc attttggaag
      541 acaaaaaaat cgaacatcct gcgagtactt tccgttatgt gactgcaagc tttggtgtgg
      601 cctgtaccat tccttttaga gggttccgta gtgaagattt attaagaatt gcagatttgg
      661 ctctttatca agccaagcaa gagggcagaa actgcgtagt ccattgcgca gaaactgtag
      721 agagtttaaa attccacgct gagccacctt tacaaaaata cgcctaattc ggttacaata
      781 gccaacaata tcttctattg ggttgcgtgt gtctacacag gtcggaatta tttccaagct
      841 aacagcagag catactactc ctattaacta ttttttgcac ttaggggccg atcccatttc
      901 cttaaatggt ttgttgggaa aaaagctacg tctgaggttt acgggcaaaa ttcattgtaa
      961 tcattgcgca cgaccgataa aaaaaaccta tcgaggctat tgttttcctt gcagccaaaa
     1021 attagccagt tgtgacttat gtattgttaa accagaactt tgtcattttc atttaggtac
     1081 ctgtcgagat ccccgatggg cttcccaaca ttgcatgatc ccgcattata tctatttagc
     1141 caatacgtcc gagttgaaag ttggaataac gagaaaaaat catattccga atcgttggtt
     1201 agagcagggg gcaacttcag ctttgcctat tatcgaagta aaaacgcgtc aacaatctgg
     1261 ttttgtcgaa gagattttta aacaatttgt taaggacaaa accaactggc gccaattatt
     1321 gcgttgtgag aatgaaaatg tggatcttcg tgcgctagcc agtatcttac ttgaaaaagc
     1381 tgatactgga ttgcagaaag ttttggaata ttatggcaaa gagaatataa ctctattgaa
     1441 agatccgttt ctctttcaat ttagttatcc agtaatccaa tatccacgaa aactgaagag
     1501 tttggcatta gacaaagaaa aagaaataag tgataccctt ttaggattaa aaggccaata
     1561 ttttattttt gatgagggcg ttttaaatat acgtaaacat acgggatttg aaattgagtt
     1621 tgaagcaatt aactaacatt tgcttcgcta acaggtggac attgatacac taattcaatg
     1681 gttccatcat gttgaaccgt ttctaatttt actggaaatc cccaaagcgt atgcacatga
     1741 cgtaaaactt cttcggtatc ctcagaagat aaaggccttc ttttgttttg ggtataccgt
     1801 aaagtaagag aacgatcgcc gcgtttatct actttataaa cctgtatatc gggatcaata
     1861 ttgccgatat tataattttc agacaataaa cgtctgacat agtgataacc ttcttcatta
     1921 tgaataaaac taacttctaa atcttctttt tgatcgtcat ctaatatgca aaataatttt
     1981 aaatcgcgta ctacctttgg tgaaagaaat tgagcaataa agctttcatc tttaaagttt
     2041 ttcattgcaa aatctagtgt ctgaatccaa ggttgccctg caaaatcggg gagccatcgt
     2101 ttgtcttctt ctgtggggtt ttcacatagg cgacgtagat cactatagat cgaaaaccct
     2161 aaggtgtagg gatttaagcc attatagtaa gggctttcat acgtaggctg ataaatgacg
     2221 ttggtatgcg aatgtaaaaa ttccatcata aaatcatcat taaccaatcc ttcatcgtat
     2281 aattggttta ataacgtata atgccaaaaa gtagcccagc cttcattcat gactttagtt
     2341 tgtcgttgcg gataaaaata ttgggccatt tttcttacaa tacgcacaat ttcacgttgc
     2401 caaggctcta gaaggggggc atttttttca ataaaataaa gaatattttc ttgtggttct
     2461 gctggataaa tttcttcttt cacatctttg gtttcttttt tgcgttgggg taaagtgcgc
     2521 caaagttcat tgaccaacga ttgcatataa tctgcccgtt ttgcttgacg aattttttct
     2581 tcacgcaaag agattttatt gggccgtttg tagcgatcaa ccccttgggt catcaaagca
     2641 tgacatgcgt ctaaaatggt ttctacttcg tccatgccat atctttcttc gcattcggca
     2701 atataatttc gcgcaaatac cagataatcg acaatggaat ctggactagt ccatgtttta
     2761 aataagtagt tatttttaaa aaaagaatta tggccatagc acgcatgggc aagcactaaa
     2821 acttgcatgg ttaaagtgtt ttcttccatg agataagcaa tacatggatc ggaattaata
     2881 accaattcat aggccaatcc catatggcct cttttataat ttttttctac cgttaaaaat
     2941 tgctttccaa aagaccagtg attatagcct aagggcattc cggtcgatga ataggcatcc
     3001 atcatttgtt ctgcagatat tacttcaatt tggtttgggt acgtgtcaag tccaaatgtc
     3061 ttagcgagct tgcctaattc ttgatcaaat gttgtgagta attcaaaatt ccattcgtta
     3121 gaagtggcta aaggtctcga tgatttagct gatttgcttt tcatacattt cttacgatgt
     3181 tatagattct tgacgcttaa ataattctcg aaatacggga taaatgtctt taatttcttc
     3241 tatatgctgc atagcaaatt gtggcgagct ttcttttacc gttaaatacg cttcccacaa
     3301 actttggtga tggcgcggca taatttctac gtaagcaaaa tattgtacat ggggcaaaat
     3361 atcgtataaa agtaaatcgc gacagcgcgg agaatcattg ttccaattgt caccatcgga
     3421 tgcttgggcc gcatagatat tccaatcggt ggtagggtat cgactcatca taattttttt
     3481 cattaattca agcgcactag aaacgactgt acctcccgtt tcgcgtgaat aaaaaaattc
     3541 ttcttcatcc acttctttgg ctgaggtatg atgtcgtata aaaaccactt ccacattttc
     3601 ataattacgt gataaaaata aatataacaa tataaaaaaa cgttttgcta tatctttttt
     3661 ggcttcatcc atagagccgg aaacatccat caaacagaac ataacagctt gtgtcgtggg
     3721 ctttggttgt ctaacacgat tgttatagcg caaatcaaaa ggatcgatga aggggatcgc
     3781 gtgtaaacgt tttttgagta attcaatttc ttccttcaat tcggtaactt tgggcgactc
     3841 cggatgaatc gctaataagt ttgctaattc gagctctagt tgctccaatt tttctttaga
     3901 aggggcactt aaggccactt ttctagcaac ggcgcctttc aaagaacgta ctatattaat
     3961 attgctcgga tttccctcat gggtaaatcc agcacggatc attttaaatg cgggtatttt
     4021 tgcgatttgt gttttgatta aattaggtaa ggccaagtct tcaaaaaata attctaaaaa
     4081 ttcttctttc gaaatttcaa aaacaaaatc gtccatacct tcggcaccgt tcgaggcttt
     4141 accgcctcgc ccattaccac tattgcgtgg aggacgtttt aataaatccc cgggcacaaa
     4201 ttctttattg cctggcaata tgagatgacg ttcacctcca tcaccataac gaaactgcgg
     4261 ttcattgata tcttttgcag gaatataaat tttttctccg ctttcatcta cctctttgat
     4321 ggaacgtccg gtgatagcat gtcgaaccgc ttcctgtatt tgccgtttat aacggcgcat
     4381 gaaacgttgc cgatttacgg cacttttatg tctaccattc ggtcgtcgat caataaaacg
     4441 tgacatataa gttcctttat actcttcgca gatgcatttt cgagggtgtt gtctctcctc
     4501 ctgcgcgcag gagctcgatg tctacccgaa aatcatctac tttgagtata ttaacgttta
     4561 ggaagatttt cgcacgcgca agtaccattc gcataataaa cgcacttgtt tgggcgtata
     4621 ccccttatct accatccgat ccacgaaatc ctgatgttta cgtttttcat ccgcggaagc
     4681 ttttgtgtta aaagaaataa ccggtaacaa atcttcggtg ttggaaaaca ttttcttttc
     4741 gatgacagca cgcaatttct cgtaactagt ccaacttggg tttttgccac ggttatttgc
     4801 tcgcgcacgc agtacgaagt taacaatttc gtttctaaaa tctttcggat tactgatacc
     4861 cgcgggcttt tcgatttttt cgagttcgtc atttaaggaa cgacgttcca aaatttcacc
     4921 ggtatcggga tcgcgatatt cttgatcttg aatccaaaaa tctgcataag tgacatagcg
     4981 atcaaaaatg ttttggccat attcagagta cgactcaaga tatgcagttt gaatttcttt
     5041 accgataaat tcaacataac gcggtgataa aaatccttta atataagaca tatagcgttc
     5101 gtgcaattcg ggaggaaatt gttctcgttc gatctgttgt tcaattacgt atagaaggtg
     5161 cacagggttc gccgccacct cggtattatc aaaattaaat actttcgata aaattttgaa
     5221 agcaaatcgc gtagaaagtc cggtcatgcc ttcatcaata cctgcaaaat cgcgatattc
     5281 ttgataagat tttgctttgg gatcggtatc ttttaaattt tcgccgtcat aaacgcgcat
     5341 ttttgaaaaa atgctagaat tttctggctc ttttaatctg gaaagtacag aaaattgcgc
     5401 catcattttt aaggtgttgg gagcacaagg cgcatcgcgt agagaactgt tttgtaataa
     5461 cttatcataa atttttattt cttcggtcac acgaagacaa taaggcacct tgacaatata
     5521 aacacgatcc agaaatgctt cattgtgctt attattgcga aaagtttgcc actcactttc
     5581 gttggaatgt gccataataa tgccatcaaa tggcatcgaa gcagagcctt ccgttccatt
     5641 ataatttcct tcctgggtag cagttaggag cggatgtaag actttaatgg gagccttaaa
     5701 catttccaca aattccaaca tacctcggtt tgccttacaa agcccacccg aaaaactata
     5761 cgcatcggca tcgtcttgtg catatttttc gagttttctt atatcgacct tgcccactaa
     5821 agaggaaata tcttgattat tttcatcccc tggctctgtt ttggcgatag caatttgtcg
     5881 aagtcgactc ggccacaatt taacaacctt aaactgatta atgtcaccat tgtattcatt
     5941 taaacgcttt acagcccaag gtgacattat cgcttctacg taacgtctag gaacaccgta
     6001 ttcttcttct aaaatgggac catcttcaat aatatcaaac aagcctaaag gagattcgaa
     6061 aataggtgaa ccttttaagg cgtagaaagg cacatgttca ataagcgatt ttaatcgctc
     6121 agctaaagaa gatttgccac ccccaaccgg acctaacaaa tataaaatct gctttttttc
     6181 ttctaagcct tgggcagcat gttttaaaaa agaaactatt tgatcaatgg gttcttccat
     6241 gccataaaat tcatgaaaag aatcgtagag ttttatgact ttgtttgaaa aaattctgct
     6301 gagtctggga tctttgctgg tatcaactaa gcgaggttcg ccaataacat gcaataatcg
     6361 ttcagcagcg tttgcatagg ccattcgatc gtgtttgcaa cgttccagat attcctgaag
     6421 agtcaattct tcttcttgcg ttttgccata atagcgatct ttaaaattgc tcaatacgtc
     6481 catgaggtct ccttcctggc aattttccca tcttaagaac attgtatgtc cctaatctgc
     6541 ggtgaatcaa gctctttaat attatctttc gtgcc
//
LOCUS       DASTIB010000285         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_806965, whole genome shotgun sequence.
ACCESSION   DASTIB010000285 DASTIB010000000
VERSION     DASTIB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806965"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VFP93_04930"
     CDS             <1..1343
                     /locus_tag="VFP93_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843981.1"
                     /translation="LYDLKLQNAHISFFSGLYLQGNVIEALASGNPITNALSTGLDDF
                     KKAAQYYYHDKPIHAYEALFNGLKSIVMSAAPFAALLQAGLSNHQTMVAVLSLHVAIF
                     LKWHNKLIDFKKIFTKDRQQTLYNYYLALMEGKLTKAERIAKELLTKHQLDVNDFSLL
                     SEIEEVAITHVREQMLNILVRGLLNNIQKCQRSSAQNFATFDRSLRSAKYLKEQTKYI
                     KRLQPFVDLTANNDAELDNLIKKTNKPAVQGLFQQLKHARHQSAITSLSQPTRFQVPH
                     LKKQLANNKQIGKELNILLENYTYTSELYKDIYAVMSQLKQTDPFKFLSFQRKIAECD
                     PLLSLKIFQNLTDQNSEMVYCNQDTLSTCLQQLDNYALLQDPFYEQECTQIFGQLLKQ
                     PHLQNAFKQSLMQASKTLQENLLGIAQKYKAKALYLCITDALNTSTSRAMAGCK"
     gene            1593..2987
                     /locus_tag="VFP93_04935"
     CDS             1593..2987
                     /locus_tag="VFP93_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9843982.1"
                     /translation="MSLGNHDKSKDAKTLTRHPTGSIRELWTLSYPLMLSALSGTLML
                     FCDRIILAHYHTDAMNAAASASMVFFTFIFAGISIAGIAEVLVGQYNGAQKWEKIAEP
                     VWAMIWFSSFLVIPFIGIGIFGSPYLLANNMQEYGVPYLTGLMFSAPLFALVAALSAF
                     FIGRGQVALVTYSVAIGNVLNFVLNLVFVFGVEGYIPAMGTLGSSLGTGIAQATIALI
                     LFALFLSKTNRVKFGTGNWRLHWGSFRQCMRIGLPNSLGHMAAITAWTIVLHLLASTS
                     FEHITVFTIGQSIWILFSFITDGSSKAMSAVAANLIGSNNIHKIKKVLVSGILLQCIY
                     AVFLAVPLLITPHLMIDLFIPDVTDTHIQSIKSLLAAGCFWLWIAFVGDAITWVIAGI
                     LTAAGDTIFIMIMSTLASWIFGMLPIYIMVVKYHGAPNVSLNLIALFTTLTAISFYLR
                     YKTNHWERKALLIA"
     gene            3068..>3277
                     /locus_tag="VFP93_04940"
     CDS             3068..>3277
                     /locus_tag="VFP93_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843983.1"
                     /translation="MVRDLTSCLIAFVLLCTSTLSLAQMEMPPVPVEAMHPKKEKILD
                     TIETTGVLKSQSSIWITSETPGRITH"
BASE COUNT          987 a          634 c          595 g         1061 t
ORIGIN      
        1 ccctctatga tttgaaatta cagaatgcgc acattagttt ttttagtggt ttgtatctcc
       61 aaggtaatgt tattgaggca ctagcaagtg gaaatcctat taccaatgcg ctcagcacag
      121 gtctggatga ttttaaaaaa gcagcacaat attattatca tgataaaccc atccatgcat
      181 acgaagcact ttttaatggt ttaaaatcga tagttatgag tgctgctcct tttgctgctt
      241 tactgcaagc ggggttaagt aatcatcaaa ctatggttgc tgtgctctct cttcatgtcg
      301 ctattttttt aaaatggcat aataaactca ttgatttcaa aaaaatcttc accaaagatc
      361 gccaacaaac actttataat tattatttgg cactcatgga aggaaaactt accaaggcag
      421 aacgtattgc aaaagaattg ttaacaaaac accaactgga tgtaaacgat tttagcttac
      481 ttagtgaaat cgaagaagtt gcaattaccc atgtgcgtga gcagatgctg aatattcttg
      541 ttcgaggctt gttaaataac atacaaaaat gtcaacgcag tagtgcacag aattttgcca
      601 cttttgatag gtccttacga agcgccaaat atttaaaaga acaaactaaa tatataaagc
      661 gcttgcaacc cttcgtggat cttactgcga ataacgatgc agagttggac aatttaatta
      721 aaaaaacaaa caaacctgct gtacaaggtt tattccagca gttaaagcat gcgcgccatc
      781 aaagcgcaat aacctcgctt tcgcaaccca cgcggtttca agtgcctcat ttaaaaaagc
      841 agttagcaaa taataaacaa ataggtaaag aattaaacat tttattggaa aattatacct
      901 atactagcga attgtataag gacatttatg ctgtaatgtc gcaattaaag caaactgatc
      961 cattcaaatt tttaagtttt caacgcaaaa ttgccgaatg tgatccctta ctttctttaa
     1021 aaattttcca aaatttaacg gatcaaaatt ccgaaatggt gtattgtaat caagatactt
     1081 tatcaacgtg tttacaacaa ttggataact atgctctttt acaagatcct ttttatgaac
     1141 aagaatgcac acagattttt ggtcaattgc tgaaacaacc acacttacaa aatgctttca
     1201 aacaaagctt aatgcaagca tcaaaaacac tacaagaaaa tttacttgga attgcgcaaa
     1261 aatataaggc taaggcgctt tacctgtgca ttacagacgc tttaaataca tctacttctc
     1321 gggctatggc gggatgcaag taaccttgat tttccaaacc ttagtctatt tttggaataa
     1381 tttatcttaa aaacataatg tcttatccaa agttcttaaa aaaatagtga cataatttaa
     1441 agattatgtc ataatttccc ctattttccg cattatacac ttgttttcat taaaaaagga
     1501 gtttctcatg caaaccaacg ttttacgcat taatgcgaat ttatcaaaca actttttagc
     1561 tgcgcgaaaa ccggtaacgt tatggagatt ttatgtcttt aggaaaccac gataaatcca
     1621 aagatgctaa aacacttacc cgacacccca cgggaagtat tcgtgaatta tggacacttt
     1681 catacccttt aatgctctca gcgctgtctg ggacattaat gctcttttgt gatcgaataa
     1741 ttttagcgca ttaccataca gacgcaatga atgctgccgc aagcgcgtcg atggtgttct
     1801 tcacctttat ttttgcaggt atttctatcg caggtattgc ggaagtattg gtgggacaat
     1861 acaacggtgc gcaaaagtgg gaaaaaattg cagagccggt ttgggccatg atttggtttt
     1921 cttcattttt ggttattcct tttatcggca ttggaatttt tgggtcccct tacttacttg
     1981 ccaataatat gcaggaatat ggcgtcccgt atttaacagg attaatgttt tcagccccct
     2041 tatttgcgct ggtagctgca ttatcggcct tctttattgg tcgtggccag gttgccttag
     2101 taacttactc cgtcgccatt ggtaatgtat taaattttgt gcttaatctt gtgttcgtat
     2161 ttggcgttga aggctatata ccggctatgg gcactttggg ctcaagtctg ggcacaggaa
     2221 tcgcccaagc aacgattgcg cttattttat tcgctttatt tttaagtaaa accaatcgcg
     2281 taaaatttgg cacaggtaat tggcgcttac attggggttc ctttcgccaa tgcatgcgca
     2341 ttggtttacc caattcttta ggtcatatgg ctgctatcac tgcttggacg atagtattac
     2401 acttattagc atccaccagt tttgaacata taacggtttt tacgattggc caaagcattt
     2461 ggatattatt ttcgtttatc accgatggta gttctaaagc catgagtgcg gtggcggcca
     2521 atttaattgg ctcgaataac atccataaaa ttaaaaaagt gttagtttct gggattttat
     2581 tacaatgcat ttatgctgtt tttttagcag tacctcttct aatcacgcca catctgatga
     2641 tcgatttatt tattccggat gtgactgata cccacatcca atcgatcaaa agcctattag
     2701 cagcgggatg tttttggctc tggattgctt ttgtaggcga tgctattacc tgggtcatag
     2761 caggtatttt aaccgcagcc ggtgatacca tttttattat gattatgtcg acgctggcta
     2821 gttggatctt tggtatgctg cccatttata ttatggttgt gaaatatcac ggtgcaccga
     2881 atgtctccct taaccttatt gcgttgttta ctacgttaac agcaatttcg ttttatcttc
     2941 gttacaaaac aaatcattgg gaacgtaaag cacttttaat tgcgtaagag ctaccgaaaa
     3001 tctgcttttt tactagaagt taggaatcga atttgatata agctatcaaa attgtccaag
     3061 gattcgtatg gttagagact taacttcttg tttgattgct tttgttcttt tatgcaccag
     3121 cacactctcc ttagcacaaa tggaaatgcc accggtgcct gtcgaagcga tgcaccctaa
     3181 aaaagaaaaa attcttgata ccattgaaac taccggcgtt ttaaaatccc aaagttccat
     3241 ttggataact tccgaaaccc caggtcgtat tacccat
//
LOCUS       DASTIB010000286         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_541929, whole genome shotgun sequence.
ACCESSION   DASTIB010000286 DASTIB010000000
VERSION     DASTIB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541929"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..911
                     /locus_tag="VFP93_04945"
     CDS             153..911
                     /locus_tag="VFP93_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET9843984.1"
                     /translation="MKKFITMVFGIIFLCGCQKETADNTWVVATNATYAPYVSIKDGK
                     IVGFDMDVAKIIAEKLHKKIEIKDMGFDSLILALQQNKVDMIIGGISITPSREKEIFL
                     IPYQGKPVTHFTLVLWEKPNRSIEKFEDLVGLHIAVQTGTIQQTFLERYPSIKIDSLE
                     GNTETLMNVQYKKSFAALVEPVIAQEMQLKFPHLTLIDLPLPEEFWALGNGIGVAKNN
                     PEKRDRVAQLIEDMQKEGILAQLEQKWLKVQTHD"
     gene            904..1563
                     /locus_tag="VFP93_04950"
     CDS             904..1563
                     /locus_tag="VFP93_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET9843985.1"
                     /translation="MIDYGLIIQSVPLMLQGALVTLEICAVALMIGFVAGVFLGTLNS
                     PKVARKPIRLMIKAYVAIIRGTPLFVQVLIVYFGLPELTHINLSPFAAGVLALSCNTS
                     AYLAEIVRAGMVSVPYQQWDAGFTLGYKKIHIITLLILPQALRKIWPAITNEFVTLIK
                     ESSILMILGVSEITKISKDIVSRELKPMTIYVLTGCIYFALTYTISKLASKWEAKHEY
                     S"
     gene            1550..2272
                     /locus_tag="VFP93_04955"
     CDS             1550..2272
                     /locus_tag="VFP93_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9843986.1"
                     /translation="MNILEVKNLSFNATHQIILQQINLTIEKGRSTILIGQSGSGKTS
                     LLKCMVGLIQNYQGNILLQGKEVKSLPPQERVNWLGFVAQGFHLFPFMNILENCMHPL
                     QKVFKKNYSDAKQKALEVLSELKIEHLAHAYPSTLSGGQQQRAAIARALVMDSKILMM
                     DEPTSALDPNATANLHQLLQQLQERGLTLVITSHDMNFVKKFKDRVFFLEQGLLVESW
                     DFRHSLDSCGTRIQSFLDMESN"
     gene            2345..2560
                     /locus_tag="VFP93_04960"
     CDS             2345..2560
                     /locus_tag="VFP93_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843987.1"
                     /translation="MDNLIMKNILSNELSLISGGNQEEVMPVQNTNIEPAGMKSIFVF
                     FMDNNPEESMKVLFSEIENQTCQDDEV"
BASE COUNT          834 a          474 c          521 g          804 t
ORIGIN      
        1 tttagggtta agtatcaaca attccgcaat cgctactcgc tctatccgac cacgccgtat
       61 ccgcggctag aatttctttt tccgtacatc accttcttca tggtttaatt ttattccatt
      121 aagaatcata tgctcagcgc ggggtttttt ttatgaaaaa atttattact atggtatttg
      181 ggattatttt tttgtgtggt tgtcaaaaag aaactgcgga taacacgtgg gtggtggcaa
      241 ccaatgcaac gtatgcgcct tacgtgtcta ttaaagatgg aaaaatagtg gggtttgata
      301 tggatgttgc aaaaattatc gccgaaaaat tacacaagaa aatcgaaatt aaagatatgg
      361 gatttgattc cttaatactc gctttacaac aaaataaagt agatatgatc atcggtggga
      421 tttccattac accaagtcgc gaaaaagaaa tttttttgat tccctatcaa ggtaaacccg
      481 ttacccattt tacgttagtt ttatgggaaa aaccgaatcg ttcgatcgaa aaatttgaag
      541 atttagtagg tttacatatt gctgttcaaa cgggaactat tcaacaaact tttctggagc
      601 gctatccttc tataaaaata gatagtttag aaggtaatac agaaacttta atgaacgtcc
      661 aatataaaaa atcgtttgcc gctttagttg aacctgtgat cgcgcaagaa atgcagttga
      721 aatttccgca tttaacatta atcgatttgc ctttgcctga agaattttgg gcattaggga
      781 atggcatagg cgtagcgaaa aataatccag aaaaaagaga tagggttgcg caattaatag
      841 aagacatgca aaaagaagga attttagcac aattagaaca gaaatggctg aaggtgcaaa
      901 cccatgattg attatggttt aattattcag agtgttcccc tcatgttgca aggggcgctg
      961 gttacccttg aaatatgcgc ggttgctctt atgatagggt ttgtagcggg cgtttttttg
     1021 ggtactttaa actccccgaa agtggcgcgc aaacccatac gtcttatgat caaagcgtat
     1081 gttgctatca ttcgaggcac acctttattt gtgcaggtat taattgtcta ttttgggcta
     1141 cccgaactta cgcatataaa tttaagtcct tttgccgcgg gtgtgctcgc actcagttgc
     1201 aacacttcgg cgtatttagc agaaatcgtg agagcaggta tggtaagtgt gccttaccaa
     1261 caatgggacg caggctttac gttaggctac aaaaaaatcc acatcatcac cctacttata
     1321 ttaccacagg ctttgcgcaa gatttggcct gcgattacca atgaattcgt gacgcttatt
     1381 aaagaaagta gtattttgat gattttggga gtttctgaaa ttacgaaaat tagtaaagat
     1441 atcgtatcca gagaattaaa acccatgacc atctatgtgt taacaggttg catatatttt
     1501 gcattgactt atacgataag caaactagcg agcaagtggg aagcaaaaca tgaatattct
     1561 tgaagtaaag aatttgtcct tcaatgctac ccatcaaata attttacagc aaattaatct
     1621 cactattgaa aaagggcgca gtactatact tattggacaa agcggttcgg gcaaaacctc
     1681 ccttttaaaa tgtatggtcg gtttaattca gaattatcaa ggtaatattt tacttcaagg
     1741 gaaagaggtt aagagtctac ccccgcaaga aagggttaat tggttaggat ttgtggcgca
     1801 aggttttcat ctattcccct ttatgaatat cttagagaat tgcatgcatc cactgcaaaa
     1861 ggtgttcaaa aaaaattaca gcgatgcaaa gcagaaagca ctcgaagtac tcagtgaact
     1921 taaaattgaa catcttgcgc atgcttatcc ctctacgcta tccgggggac aacagcaacg
     1981 tgctgctatt gcccgcgcat tagtcatgga ttccaaaatt ttaatgatgg acgaacctac
     2041 ctcggctttg gatcccaatg caaccgctaa tctgcatcaa ttgttgcaac aacttcaaga
     2101 acgtggcttg acgttagtta taacttcgca cgacatgaat tttgtaaaaa aattcaaaga
     2161 ccgtgtgttc ttccttgagc aagggctcct agttgagtct tgggatttta gacatagtct
     2221 ggattcttgt ggtactagaa ttcaatcttt tttagatatg gaaagtaatt gagattcgat
     2281 taaccaacag tttacaggtg aagctacacg agattataat agtcctgctt attaactaaa
     2341 ttaaatggat aacctaatca tgaaaaatat attaagtaac gaattatctt taatcagtgg
     2401 cggaaaccaa gaagaagtaa tgcccgtgca gaatacaaac atcgaacctg ctggcatgaa
     2461 gtctatcttt gttttcttca tggataacaa tccagaagaa tccatgaaag ttttattttc
     2521 cgaaattgaa aatcaaactt gtcaagacga cgaagtataa ttaaattgca ttcagttttt
     2581 actgaatgca atttctatcc tatccctttt tcaatagctt cattcacgtt gtg
//
LOCUS       DASTIB010000287         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_140733, whole genome shotgun sequence.
ACCESSION   DASTIB010000287 DASTIB010000000
VERSION     DASTIB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140733"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VFP93_04965"
     CDS             complement(<1..111)
                     /locus_tag="VFP93_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140371.1"
                     /note="porin involved in osmoregulation allowing water to
                     move into and out of the cell in response to osmotic
                     pressure; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET9843988.1"
                     /translation="MSLIKRMSAEFIGSAWLVLGGCGSAVLSAAYPELGIG"
     gene            complement(237..>2068)
                     /locus_tag="VFP93_04970"
     CDS             complement(237..>2068)
                     /locus_tag="VFP93_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HET9843989.1"
                     /translation="LEANFNIKFDRKQSSVLDSIFQSFIGAKHIEKVAHDVGGKYAAI
                     TISGKAHQPETQGFNPGDATKGRSSNKRTTDGDGFPTVALYYGHNKNITQQMVIQSGS
                     TIVGGESGLINLTNGDLNAIVSFYNDTISKNIPVVVNCTDGIDRTGVIMAALQILHAY
                     KTGKDITQLLQTQQQQEMLTIIDDLRRDRGPKFLQGTEYVAMAVTLGYSLVQTQKQIE
                     FQEKNHAKLVELIPTYKDLDPVVLQQEIKKTLNKKLLKSSDKQLLKDWNILVRDRNDA
                     SNQFDVTNADEFCKLFRNFGVDRKKVDQHLHLATVGTHEIDKAPISKYNEALKAGFDI
                     GSYYINGQNIYNFAIKTLRAKPDEGDNAAEFARLLHHTVKMKDKTNLTVLKNELANFG
                     YSEMKMVKNALVDYEKKHKDALQSDFKNGLKALNKLLPSDKQAQANDKKIVIDQFFKS
                     SWTKELTPSEAINVAPIKTNESTMTKNSQNHEGANTNNGARRTLIYQFSQKMETTPLT
                     KASPIPPIQKLLDILETGLNALVNSTHNDSVGREALSKIMQAFKSNGVQGEDENKQKL
                     KDLVKELKAIPPSETQEKIAKILETLHVDHILSKLDNRPSLRK"
BASE COUNT          576 a          378 c          328 g          786 t
ORIGIN      
        1 tccaatgcca agctcagggt aggctgcaga aagtaccgca ctcccacatc ctcctaaaac
       61 caaccaagca ctgccaataa attcagcaga catccgtttt attaacgaca taacctattc
      121 tccttgtaag attgctattt catagcatgc ataaatgatg gaagcaagaa gtctttttat
      181 ttgattttaa caaactaact gaatgcgagc ttgaagttat taaggcatag gaggtactat
      241 ttacgtaacg aaggacgatt atctagttta gataatatat gatcaacatg aagagtctct
      301 aaaattttcg ctattttttc ctgcgtttct gacggaggta ttgctttcaa ttcttttacc
      361 aaatctttta gtttttgttt attttcatct tcaccttgca caccattgct tttaaatgcc
      421 tgcattattt tagatagagc ttctcttcct acgctgtcat tatgggtaga gtttacaagt
      481 gcatttaatc cagtttccaa aatatctaat aatttttgaa tagggggaat aggagatgct
      541 ttagtcagtg gagtagtttc cattttttga gaaaattgat aaataagcgt tcgacgtgca
      601 ccgttattgg tatttgctcc ctcatgattt tgcgaattct tcgtcattgt tgattcattt
      661 gttttaattg gagctacgtt tattgcctct gaaggagtta attcttttgt ccatgatgat
      721 ttaaaaaact gatcaattac aattttttta tcatttgctt gagcctgctt gtcagaaggt
      781 aacaatttat ttaacgcttt taatccattt ttgaaatctg attgtaaagc atctttatgt
      841 tttttttcat aatcaactaa agcatttttt accattttca tttcgctata gccaaaattt
      901 gctagttcat tttttagtac agttaagtta gttttatctt tcatcttcac cgtatgatgg
      961 agcaaacgcg caaattccgc tgcattatca ccttcatccg gttttgcacg taaagttttt
     1021 atggcgaaat tataaatatt ttggccattt atataatacg agcctatatc aaaacctgct
     1081 tttagcgctt cattgtattt actaataggt gctttatcaa tttcatgcgt acctacagta
     1141 gctaaatgaa gatgttgatc cactttcttc ctatcaacac caaaatttct aaataattta
     1201 caaaattcat cagcattggt cacatcaaat tggttgcttg catcatttct atctcggact
     1261 aaaatattcc aatctttaag taattgttta tctgaagatt ttaataattt tttattaagt
     1321 gtttttttta tttcttgttg aagtacaacg ggatccagat ctttgtatgt cggaatgagt
     1381 tctactaatt tagcatggtt tttttcttga aattcaattt gtttttgcgt ttgaactaag
     1441 gaataaccaa gggttactgc catggctaca tattccgttc cttgtaaaaa tttaggacct
     1501 ctatctctgc gtaaatcatc aataatcgtt aacatttctt gttgctgctg cgtttgcagt
     1561 aattgtgtta tatcttttcc agttttatag gcatgtagta tctgtaatgc ggccataata
     1621 acacccgtgc gatcaatacc atcggtacaa tttactacta ccgggatatt cttcgaaatt
     1681 gtatcattgt aaaacgacac tattgcattt aaatcaccat tcgttaaatt tattagacca
     1741 ctttcgccgc caactatagt actcccactt tgaataacca tttgctgtgt aatatttttg
     1801 ttatgaccat agtataatgc tacggtagga aagccatcac catcagtagt gcgcttgttt
     1861 gagctcctac ccttcgttgc atcccctgga ttaaaacctt gtgtttcagg ttgatgtgct
     1921 tttccagaga ttgtaatggc tgcgtacttt ccacctacat catgtgcaac tttttctatg
     1981 tgttttgcac ctataaagga ttgaaatatt gaatctaata cgctgctttg ttttcgatcg
     2041 aatttaatgt taaaattcgc ttctaata
//
LOCUS       DASTIB010000288         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1219104, whole genome shotgun sequence.
ACCESSION   DASTIB010000288 DASTIB010000000
VERSION     DASTIB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219104"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="VFP93_04975"
     CDS             complement(<1..1886)
                     /locus_tag="VFP93_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9843990.1"
                     /translation="MLSNELEMTITSAFKEAKDKRHEFLTVEHLLLALLNDSAASNVL
                     RSCGADVDRLRRDLQTFVDETTPLIPSSEVHRETQPTLGFQRVLQRAVFHVQSAGRNE
                     VTGANVLAAIFSEQESQAVYFLKQENITRLDVVNFISHGISKVEESRELDEPDLEQHI
                     EQESTQEPSTRNPLESFAVNLNTLAKLGKIDPLVGRDHEIERTIQILCRRRKNNPLFV
                     GEAGVGKTAIAEGLAQYIVDGKVPDALVNCTVYALDLGSLLAGTKYRGDFEKRLKSLL
                     QHLRRDSGSILFIDEIHTIIGAGAASGGVMDASNLIKPMLASGDLRCVGSTTYQEYRG
                     IFEKDRALARRFQKIDIREPSVEETVKILKGLKGHFEKHHQIRYTSRALHAAAELAGR
                     YISDRFLPDKAIDVVDEAGAFQRLVPPKKRKKVIGVKEVEQIVAKMARIPSKSVSTSD
                     KTLLQNLETELTQKVYGQEKAINALATAVKMGRSGLGETDKPVGCFLFAGPTGVGKTE
                     VCKQLAHALGVELLRFDMSEYMEAHTVSRLIGAPPGYVGYDTGGLLTEAVNKAPHSVL
                     LLDEIEKSHPDVFNLLLQVMDHGTLTDTNGRKADFRHVTLIMTTNAGAQLMNRSSIGF
                     KEQD"
     gene            complement(1920..2240)
                     /gene="clpS"
                     /locus_tag="VFP93_04980"
     CDS             complement(1920..2240)
                     /gene="clpS"
                     /locus_tag="VFP93_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET9843991.1"
                     /translation="MSKEKRHENASELLLEAAKPQLKRPSMYSVIMLNDDYTPMEFVI
                     HVLESFFHMTAEVAVKVMLDVHHKGRAHCGTYSRDVAETKVAQVINFARRNDHPLLCK
                     IEKI"
BASE COUNT          626 a          520 c          429 g          722 t
ORIGIN      
        1 tggtcttgtt ctttgaatcc aatactactt cgattcatca attgcgcccc cgcattcgtg
       61 gtcataataa gcgttacatg tcggaaatcg gccttgcgac cgttggtgtc ggtgagcgta
      121 ccatgatcca taacttgcaa taataaatta aaaacatcgg gatgcgactt ttctatttca
      181 tctaacagta acaccgagtg tggtgctttg tttacagcct ccgttaacaa tcctcccgta
      241 tcatagccca catatcctgg aggagcaccg ataagtcgag ataccgtgtg tgcttccata
      301 tattcagaca tatcaaaacg caataattct acccccaaag catgggcaag ttgcttacaa
      361 acttccgttt ttccaacacc tgttggtcct gcaaaaagaa aacagcctac cggcttatcg
      421 gtttccccaa gccccgatct ccccattttc accgcggttg ctaatgcatt gattgctttt
      481 tcttgaccat aaactttttg ggttaattcg gtttctagat tttgtaacaa cgttttatcc
      541 gaagtagaaa cacttttcga gggtatacga gccatctttg ctacgatttg ctcaacttct
      601 tttacgccaa taactttttt tcgttttttg ggtggaacca gtctttgaaa ggcaccggct
      661 tcatctacca catcaattgc tttatcgggc agaaaacgat cgctgatata acgaccggct
      721 aattcggccg ctgcatgtaa agcccgtgag gtataacgaa tttgatgatg cttttcaaaa
      781 tgacctttta atccttttaa aatttttacc gtttcttcta cggaaggttc acgaatatca
      841 attttttgaa agcgtcttgc taaagcgcga tctttttcaa aaattcctcg atattcttga
      901 tacgtggtgg aaccaacgca tcggagatca ccactggcta gcattggttt tattaaatta
      961 gaagcatcca tcaccccgcc ggatgcggcc cctgcaccaa taatagtatg aatctcatca
     1021 ataaataaaa ttgatcccga atcgcgtctt aaatgttgta aaagcgattt taaacgcttt
     1081 tcaaaatcac ctcgatattt ggtaccggct aaaagagagc ctaagtctag agcatacaca
     1141 gtgcaattaa ctaaagcatc cggcacttta ccatctacaa tatattgggc taatccttcg
     1201 gcgattgcgg tttttccgac gccagcctct cctacaaata acggattatt tttgcgccga
     1261 cggcataata tttggatcgt gcgttcaatt tcatgatcgc gccctactag ggggtcgatt
     1321 ttacctaatt ttgctaaggt gtttaaatta acagcaaaac tttctaaagg gtttcttgta
     1381 ctgggttctt gggttgattc ttgttcaata tgttgttcta aatcgggttc atctaactca
     1441 cgcgattctt ctacttttga aatcccgtgt gagataaagt taactacatc aagtcgcgtg
     1501 atattttctt gctttaaaaa atacacggcc tgactttctt gttcactaaa aattgcagcc
     1561 agtacatttg cgcccgtaac ttcattacgt cctgctgatt gcacatgaaa aaccgcgcgt
     1621 tggagtacac gttggaaccc taaagtaggt tgtgtttcgc gatgaacttc agaacttgga
     1681 atcaaaggcg tagtttcatc gacaaaggtt tgcaaatctc tacgtaacct atcgacatca
     1741 gcaccacacg atcgtaagac attggaggcg gcggaatcgt ttagaagggc caataacagg
     1801 tgctctaccg ttaaaaactc atgacgttta tccttagctt ccttaaaagc tgaggttata
     1861 gtcatttcta gttcattgct caacatagca ttatctccta atgcacatcg gttgggcttc
     1921 taaatttttt caattttgca caacaaggga tgatcattac gcctcgcgaa atttatcacc
     1981 tgtgctactt ttgtttcggc aacatcacga ctgtatgtgc cgcaatgcgc cctaccttta
     2041 tggtgcacat cgagcatcac ttttactgca acttctgccg tcatatgaaa aaaactttcc
     2101 aaaacatgaa ttacaaactc catcggtgta tagtcgtcat tcagcataat aactgaatac
     2161 atcgaaggac gtttaagctg tggtttggca gcttcgagca aaagttctga cgcattttca
     2221 tgtcgttttt ctttgctcat atcagttatc ttagtcaaag tttcaaagtt ccgcagattg
     2281 agtttataaa ctaaata
//
LOCUS       DASTIB010000289         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_543203, whole genome shotgun sequence.
ACCESSION   DASTIB010000289 DASTIB010000000
VERSION     DASTIB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543203"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..396)
                     /locus_tag="VFP93_04985"
     CDS             complement(217..396)
                     /locus_tag="VFP93_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843992.1"
                     /translation="MRTLNMKEQTSVSGGAYVPSENEIEDLSNSLGLGTWSTLFLLGT
                     LEGAVVVLMEYCFNI"
     gene            complement(585..845)
                     /locus_tag="VFP93_04990"
     CDS             complement(585..845)
                     /locus_tag="VFP93_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843993.1"
                     /translation="MRTLNFNEQIAISGGATKAEIIIGADSIATKIGVVTGWVIGGAS
                     GFEAGGAPGSFMGIALGGILGLANAKLFAPLVSIPMACVFGE"
     gene            complement(954..1211)
                     /locus_tag="VFP93_04995"
     CDS             complement(954..1211)
                     /locus_tag="VFP93_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843994.1"
                     /translation="MRTLNFIEQNTISGGATKTEVFRDTHSTIRIACIEGGAIIGGVC
                     GLAGGPIGAVAGVAVGYIGGAIYGAVLAPVLSLPIALIYGE"
     gene            1448..1957
                     /locus_tag="VFP93_05000"
     CDS             1448..1957
                     /locus_tag="VFP93_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843995.1"
                     /translation="MHLLERMIRANISEQEFLKYFEMFAKCSDVNAQVAPNIFLIHLA
                     IAHKKPLFLKALIKFGADIEALGLQNITPLQTAVALNDVESFKILVKAGANVQSLRFT
                     EYISFADFFREKAANDEKGAAAPILFANTNKNVENKNASKTKNEKYEHDVSKLKSFCN
                     KILNRPAKL"
     gene            complement(2008..2247)
                     /locus_tag="VFP93_05005"
     CDS             complement(2008..2247)
                     /locus_tag="VFP93_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843996.1"
                     /translation="MRILNIEEQKILCGGVIDYQEIVVAEKTLDILKSCLSGFFSEES
                     TARIANAAVGGAVSCAGYILYKPYMQRPLHHFFGI"
BASE COUNT          811 a          443 c          364 g          683 t
ORIGIN      
        1 agcgacaaaa gataaaaaaa tatctacagc acctgtttta ttttctcata ctaataatct
       61 ggcaaattca aatagaccga aaaacgaagc atataaacca ggtgtgccaa aatttaaaaa
      121 aggattttta gaaaaaggat tataaaatag gagttagtat aagggagaaa acatccctta
      181 tactctcaaa gttttttata gatgatttgt gtatttttag atgttgaagc agtattccat
      241 aaggaccact acagcacctt caagagtgcc cagtaaaaat agggtactcc atgttccaag
      301 tccaagtgaa ttacttaaat cctcaatttc attttcactt ggaacatatg caccaccgga
      361 tacactagtt tgttctttca tgtttaaagt gcgcatcatc caatcctcta tttcattaga
      421 aatcgcattc cgtttagata cattttgcac gtttgtctta tctaagataa aaaatccatc
      481 gattgcaaag cgtaggaata caattatgtt acgattcttt attcttacta gaaaaaatag
      541 ggaaatgtta aatgaacact tccctaaaat ttaaaatttt gcaattactc gccaaaaacg
      601 caggccattg gaatgcttac taaaggcgca aataatttag catttgctaa acctaaaata
      661 ccacctaacg caatacccat gaaactacca ggagcaccac ctgcttcaaa gccactcgca
      721 ccgccgatga cccaaccagt tactacaccg atttttgttg cgatactatc tgcaccgata
      781 ataatctctg ctttagtagc accaccagaa atcgcaattt gttcgttaaa attaagagtt
      841 ctcatattac ctcacatata ttttacagtc gtcaaggatg acttgttcgc agtatccatg
      901 aatctggatt ccgcgaataa atcgcggaaa gtagaatgtt tgaaattttt caattattcg
      961 ccataaatta gggcaattgg aagactcaat acaggcgcta atacagcacc ataaattgca
     1021 ccaccaatgt aaccaacagc cacccccgca acggcaccaa taggcccacc tgcaagaccg
     1081 caaaccccac caataatagc tccaccctct atacaagcta tacgaattgt ggaatgtgta
     1141 tcacgaaaaa cttcagtttt agtagcacca ccagaaatcg tattttgttc gataaaatta
     1201 agagttctca taaagtctca caagtattta acagtcgaca agcttaattc agctggttaa
     1261 atatgtcaac tatcgtttta ttaactaact tttgtaaggt ttttttggat tatttaacca
     1321 tgactaagcg ttttcatgat ttaagtacaa cttccgtaag gttaaagctt acaaaaatgt
     1381 gacagtttca ttaatattat acagccccgt tccactacca aattaatcaa atgcttaagg
     1441 tataaatatg catcttttag agagaatgat tcgagcgaat atttctgaac aagaattttt
     1501 aaaatatttt gagatgtttg caaaatgctc tgatgtcaat gcgcaagttg caccgaatat
     1561 ttttctcatc catttagcaa ttgcccacaa aaaaccttta tttttaaaag cgctaattaa
     1621 attcggagcc gatattgaag cattaggact tcaaaacatt acccccttac agactgcagt
     1681 agctttaaat gatgttgaat cgtttaagat cttggtaaaa gcaggtgcta atgtgcaatc
     1741 gttacggttt acagagtata tatcctttgc agattttttt agggaaaaag ctgctaatga
     1801 tgagaaaggt gctgcagcac ccatattgtt tgctaatact aataaaaatg tagaaaataa
     1861 aaatgcaagt aaaaccaaaa atgaaaaata tgaacacgat gtgtcaaaat taaaaagttt
     1921 ttgcaataaa atattgaata gaccggctaa actatagggg aatatcttta cgcgcgaaca
     1981 aaaaattccc caaatttatt agtatagtta aattccaaaa aaatgatgaa gaggtctttg
     2041 catataaggc ttatatagta tataccctgc gcaactaacg gcaccaccga cagccgcatt
     2101 ggcaatgcga gcagtagact cttcagaaaa aaagccactc aagcaactct ttagaatatc
     2161 aagtgttttt tcagctacaa caatttcttg gtagtcgatt acaccaccac agaggatttt
     2221 ttgttcttca atattaagaa ttcgcatgga tctacctctt ataattgggt gaaaagaatc
     2281 gtaattcagt tattaaattt g
//
LOCUS       DASTIB010000290         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1223509, whole genome shotgun sequence.
ACCESSION   DASTIB010000290 DASTIB010000000
VERSION     DASTIB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223509"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..760)
                     /gene="bioD"
                     /locus_tag="VFP93_05010"
     CDS             complement(80..760)
                     /gene="bioD"
                     /locus_tag="VFP93_05010"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET9843997.1"
                     /translation="MKGVFVTATGTDIGKTYLLEHLIPELALTGLVRAIKPLISGFES
                     TVDSDLHRLLRAQKIDLLPKYFEEIACYRFSAPLSPDQAASQENKKIQYEVLIDFCLK
                     ELNAKEEGLLFIEGIGGIMVPLNAEKLVIDWIGALKIPVLLIAGSYLGSLSHTLTAIH
                     ALKTQNIKILAVLVNESRDSVGLDATCNSLSPFAKYIPILSWKRGAIKTPHPLKELLQ
                     RYRFDIAN"
     gene            845..2110
                     /locus_tag="VFP93_05015"
     CDS             845..2110
                     /locus_tag="VFP93_05015"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET9843998.1"
                     /translation="MKQFNNPLWLPYTQMHNVELPPVVVATSGSQIHLEDHKILIDGI
                     ASWWSACHGYNHPCILQAMQEQLSRMPHVMLGGFTHAPALTLAQKLSELLGPDLNHVF
                     YSESGSVAVEVALKIALQYWINQDNFAKTTFMHFQHSYHGDTFLTMSLCDPEQGMHRK
                     FNHILNKHYCLSFPANVEDLLQFEVFLEKNHHMLAGLIVEPLVQAAGGMKFHTMDMLK
                     ALVLRVQDFGIPVIFDEIFTGFGRTGSLFAFEQIGVKPDIICLGKALTGGVTPLSATI
                     ASTKLYNQFLSDKVSDALMHGPTFMGHALGCAAALASCALFSQEPRLAQVQTIETYFK
                     EIFPPFARYAMVKETRVKGAIGVIQLHARLTTKQLKWLNHYFLQQGVWIRPFGDIIYL
                     TPAFTIPMHELEILIQALHFALSGLQNLL"
     gene            2172..2711
                     /locus_tag="VFP93_05020"
     CDS             2172..2711
                     /locus_tag="VFP93_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9843999.1"
                     /translation="MTWEEIKQKSAAIRAKVTNWFKSQEIFMSTAFKKYQKNIDPWTK
                     HTYRFSIAFYTICAAYQACVTLIPWALSVGALFVTFPQFFFTLPTPLATLLFLGVSLV
                     VGYFKYKNLLHQERLEQLVEEHEEKIHQLVKQIQELQPKVPVFTPAHDHVKSRTMIVN
                     NENPSIDPQNAQFPILKPH"
     gene            2864..3250
                     /locus_tag="VFP93_05025"
     CDS             2864..3250
                     /locus_tag="VFP93_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET9844000.1"
                     /translation="MTYAYWYLFLVMLLPYSFALLAKFGHTEFNNKKPRDFLENLEGW
                     RKRSHWVQLNSYEMFAPFAASVIIAHLAQVPQQRIDVVALVFLILRVLYGLFYIWNKS
                     LLRSLSWFCALGCIVTLYILASWVTA"
BASE COUNT          962 a          662 c          650 g         1043 t
ORIGIN      
        1 attaatggaa tcaagaaatc aagtagttta tcaaaaaagc taacctgtgt gaaaataaaa
       61 ataatcccgg attttcattc taattagcga tatcgaacct atatctttgt aatagttctt
      121 ttaacgggtg aggggttttt attgcccccc ttttccatga caggatcgga atatattttg
      181 cgaaaggcga aagactgttg caagtcgcgt ctaagccaac agaatcccta gattcattta
      241 cgagtaccgc taaaatttta atattttgtg tcttgagtgc atgaatcgcc gttaacgtat
      301 gacttaacga tcctaaataa cttcctgcta taaggagaac cggtattttc aaagcaccta
      361 tccaatcgat cactaatttt tctgcattaa gcggcaccat tatgccacca atcccctcaa
      421 taaaaagcaa accttcttct ttcgcgttaa gctcttttaa acaaaaatcg atcagcacct
      481 catactgaat ttttttattt tcttgcgagg cggcttgatc cggcgataag ggtgccgaaa
      541 atcggtagca tgcaatttct tcgaaatatt ttgggaggag atctattttt tgtgcacgta
      601 aaagccggtg gaggtccgaa tccactgtgg attcaaaacc ggatatcaaa ggctttatgg
      661 ctcgcaccaa acccgtcaaa gcgagttctg ggatcaggtg ttctaatagg taagttttgc
      721 ctatatctgt acccgttgca gtgacaaaaa ctcctttcat gataagttaa ccaactcgct
      781 attgtgtttt tctattttaa cgaacaatcc aattttggaa aagttgttcg gtataatgct
      841 gggtatgaaa cagtttaaca atcccttgtg gttgccctac acccaaatgc ataacgtgga
      901 gttacctccc gtggttgtcg ccacttcggg ctcgcagata catttggaag accataaaat
      961 attgatcgat ggtattgcct cttggtggag cgcctgccac ggatacaatc atccttgcat
     1021 actgcaagca atgcaagagc agctttcgcg catgccccat gtgatgttgg gcgggtttac
     1081 ccatgcgcct gcattaacgc tggcgcagaa gctcagtgaa ctcttagggc ccgatctcaa
     1141 tcatgtattt tattctgaat ctggttccgt agccgtcgaa gtcgctttaa aaatagcgtt
     1201 gcaatattgg ataaatcaag ataatttcgc caaaacgacg tttatgcatt ttcagcattc
     1261 ctaccatggg gacacttttt taaccatgtc cttatgtgat ccagaacaag gtatgcatcg
     1321 aaaatttaac catattttaa ataaacacta ttgtttatcc tttccagcta acgtagagga
     1381 tttattgcaa ttcgaagttt ttttagaaaa aaatcatcat atgcttgcag gcctcatcgt
     1441 ggaaccgtta gtgcaagctg cgggtggcat gaaatttcac accatggaca tgctcaaggc
     1501 gctagtatta cgcgttcaag attttggcat tcccgtcatt tttgatgaaa tttttacagg
     1561 ttttggaaga acaggaagtt tatttgcctt tgaacaaatt ggcgttaaac cggatattat
     1621 ttgtttaggg aaagcattga ctggcggtgt tacgccttta agtgcaacca ttgcaagcac
     1681 taaactttat aatcaatttc tttccgataa ggtaagtgat gctttgatgc atggtccaac
     1741 ttttatgggg catgcgcttg ggtgtgcagc agcgctagct tcgtgcgcac tgttttcgca
     1801 agaaccaaga cttgcacaag tgcaaaccat agaaacctat tttaaggaaa tttttccacc
     1861 cttcgctcgc tatgcgatgg ttaaagagac gcgcgtgaaa ggtgctatag gcgttattca
     1921 attgcatgca aggcttacaa caaaacaact gaaatggtta aatcactatt ttttgcagca
     1981 aggcgtttgg atcaggcctt ttggcgacat aatatattta acgcctgctt ttaccattcc
     2041 tatgcatgaa ttagaaatac ttattcaagc gttacatttt gccctaagtg ggttacaaaa
     2101 cttactttaa tttgagttta aaggataact ttgctactat tgccttcttt tcaatgagca
     2161 ggtcttacag catgacttgg gaagaaatta agcaaaaaag cgcagcaatt agagcaaaag
     2221 taacgaattg gtttaaaagc caagaaatat ttatgagcac ggcttttaaa aaataccaaa
     2281 aaaatatcga tccctggacc aagcatacct atcgttttag cattgcgttc tatacaattt
     2341 gtgctgctta tcaagcatgc gtcactctca ttccctgggc actttcggta ggtgcacttt
     2401 ttgtaacttt tccccagttc ttttttaccc ttcctacacc gttagctacc ttattgtttt
     2461 tgggtgtatc tttggtggtt ggttatttta aatataaaaa tttattgcac caagaacgtt
     2521 tagaacaatt agtggaagag catgaagaaa agattcatca attagtcaaa caaatacaag
     2581 aattgcaacc taaagtacct gtgtttactc cagcgcatga tcatgtgaaa tcgcgtacaa
     2641 tgatagtaaa taacgaaaat ccaagcattg atccacaaaa tgcccaattt cctattttaa
     2701 aaccgcatta aatactaaag gtatctagag ttttactcga tccctctttc ataattaaga
     2761 gggatgacta tctacttggg gaagttttcc gcaccactgt ggcatacaaa gattgcttag
     2821 cggtaaaata gtccgaaggg taacgtgtaa caaaggattt agaatgacct atgcatattg
     2881 gtatttattt ctcgtaatgc ttcttcctta tagctttgct ttattagcta aatttggtca
     2941 caccgagttt aacaataaaa agccacgtga ttttctcgaa aatttagaag gttggcgcaa
     3001 acgctctcat tgggtacaat taaattctta tgaaatgttt gcacccttcg cagcaagcgt
     3061 cattatcgcc catctagcgc aagttcccca acagcgaatc gacgtggtag cccttgtgtt
     3121 cttaatttta cgcgttttgt atggattatt ttacatttgg aataagtctt tattaagatc
     3181 attatcctgg ttttgcgcac tcggatgcat cgttactctt tatattttgg caagttgggt
     3241 aacggcttaa tgttgcacgg gttgcaatga aaaattcttc acggtatccg gtgttaaaag
     3301 tcgtattgcc aagaccg
//
LOCUS       DASTIB010000291         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1214518, whole genome shotgun sequence.
ACCESSION   DASTIB010000291 DASTIB010000000
VERSION     DASTIB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214518"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1310
                     /gene="rsmB"
                     /locus_tag="VFP93_05030"
     CDS             30..1310
                     /gene="rsmB"
                     /locus_tag="VFP93_05030"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET9844001.1"
                     /translation="MNYRLYVALLLKNVLGENKNFETSDPIFKNIDAKEQSKIKFLCF
                     TLLRNYYYFEEIAKRLLSKPLKKKDYDIFGLLLLGLYELSQSMDTAYAIVHETVACAN
                     HVKKDWSKGLLNATLRRFIREHDTLCQNLPKIAQTNHPQWLLTKIQKQWPEYEKQVLE
                     ANQKLPPLTLRVNQQQIARKEYLTVLNQEHIEALPLDDANHAVMLKQALNVLEIPGFA
                     NGAVSIQDLSAQFAPDLLALTPHLKVLDACAAPGGKTAHLLETEPTLQLDALDIHEER
                     VGKLTSTLARLGFTATIKTADARETEKWWDQEPYDRILLDAPCTGSGVIKRHPEIKIK
                     LKPEDLKQTCALQEQLLDALWPTLKLNGILLYITCSIFQEENVMQIEKFLQKQGNAKT
                     IPLNVSWGIPQRHGQQILPTTQHDGFYYALLTKI"
     gene            1314..>2052
                     /gene="trkA"
                     /locus_tag="VFP93_05035"
     CDS             1314..>2052
                     /gene="trkA"
                     /locus_tag="VFP93_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET9844002.1"
                     /translation="MKIVILGAGQVGKALTEHLTSEENDITIVDIDAERLTELQERFD
                     VRTVQGSAAYPSTLMNAGAEDADILIAVTNSDETNMIACQVAYTLFRTPTKIARVRSS
                     EYVNYDELFGNDALPIDMRITPEHLVMEYIKRLILNPDALQVVDFANGRVQLVAVRAI
                     YGGPLVGHMISDLKKHMPHIETKIVAIYRRGHSLIPKDDTSIEPDDEVFFLATPNNIH
                     AVMSELRELGEPNRRVIIAGGGTIGYKL"
BASE COUNT          679 a          401 c          423 g          549 t
ORIGIN      
        1 tcttacttta acataggcga agtattgaca tgaactatcg tctgtatgtc gcactattat
       61 taaaaaatgt gttgggagaa aataaaaatt ttgaaacttc tgatcctatt tttaaaaata
      121 tcgatgcgaa agaacaaagc aaaataaaat ttttgtgttt taccctattg cgtaattatt
      181 attactttga agaaatagca aagcggttgt tgtcaaagcc tctcaaaaaa aaagattatg
      241 atatttttgg acttttgtta ttaggacttt atgaactttc tcaatcgatg gatacggcct
      301 atgcgatagt tcatgaaacc gtggcatgtg caaatcatgt aaaaaaagat tggtccaaag
      361 gattgttaaa cgcaacccta agacgcttta ttcgcgagca tgatactctt tgtcagaact
      421 tacccaaaat agcacaaact aatcatcccc aatggctttt aacaaaaatt caaaaacaat
      481 ggccagagta tgaaaaacaa gttttagagg ctaatcaaaa acttccgcct ttaacgttgc
      541 gtgtaaacca acagcaaatt gcccgcaaag agtatttaac agtactgaat caggagcaca
      601 ttgaagcttt accactcgat gatgcaaatc acgcagtaat gttaaaacaa gcactaaacg
      661 tactagaaat tcctggattt gctaacggag cagtatcgat tcaagatcta agtgcacaat
      721 ttgcacccga tttgttagcg ttgacgcccc acttaaaagt attggatgca tgtgcggcac
      781 cagggggcaa aacagcgcac ttattagaaa ctgaacccac cttgcagttg gatgctttgg
      841 atattcatga agaacgagtc ggaaaactaa caagtacctt ggctcgttta ggttttaccg
      901 ccacaattaa aaccgccgat gcgagagaaa ccgagaaatg gtgggatcaa gaaccctatg
      961 atcgcatcct gttagatgca ccttgcacag ggagcggggt aataaaacgt catccagaga
     1021 tcaaaattaa actaaaaccc gaagacctaa agcaaacctg tgcgctccaa gaacaattat
     1081 tagatgcttt gtggccgacc ttaaaactca atggcatact attatatatt acatgctcga
     1141 tttttcagga agaaaacgtg atgcaaatcg aaaaattctt acaaaagcaa gggaatgcaa
     1201 aaaccatacc gttaaatgtg tcctggggaa tcccgcaaag gcatggccaa caaatacttc
     1261 caacaaccca gcatgacggg ttttattacg cactcctaac caaaatataa acgatgaaaa
     1321 tcgttatttt aggggcaggt caggtcggga aggcactgac cgagcacctt acctcagaag
     1381 aaaacgacat cacgattgtt gatattgatg cagagcgtct aactgagcta caggaacgtt
     1441 ttgacgtgcg tacagtccaa gggtctgcag cttatcctag tactttaatg aatgctggcg
     1501 ctgaagatgc agatattctt attgcagtaa caaactcaga tgaaaccaat atgattgcat
     1561 gccaagttgc ttatacgctt tttagaacac ccaccaaaat tgcgcgcgta cgttccagtg
     1621 agtatgtaaa ttatgacgag ttgtttggga atgacgccct tcccatcgat atgcgtatca
     1681 cgcctgagca tttggttatg gaatacatta agcgtttaat tttaaatccg gatgcgttgc
     1741 aggtagtgga ttttgcgaat ggcagagttc aattagtagc agtgcgcgct atttatggag
     1801 gccctttagt gggacacatg ataagcgatt taaaaaaaca catgccacat atagaaacaa
     1861 aaattgtagc aatttatcgt cgaggacatt cgttgatccc gaaagatgat accagcatcg
     1921 agcctgacga tgaagttttt tttctagcca cacccaataa tattcatgct gttatgagcg
     1981 agttgcgaga attgggagaa ccgaatcgtc gcgttattat cgcaggtggt ggcactattg
     2041 gttataaatt gg
//
LOCUS       DASTIB010000292         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1361768, whole genome shotgun sequence.
ACCESSION   DASTIB010000292 DASTIB010000000
VERSION     DASTIB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361768"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFP93_05040"
     CDS             complement(<1..736)
                     /locus_tag="VFP93_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="involved in the sodium dependent uptake of proline;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HET9844003.1"
                     /translation="MSILTIIAFCAYLLVVVIIALTAYFVTKNLSDFVLGGRSLGGPV
                     AALSAGASDMSAWLLLGLPGAVFSFGLNQIWLPIGLTIGAYISWRILAKPLRLFSEIA
                     QDSLTVPAYLDNRFFDRSGIIRISLALITLTFFAFYTASGLVAAAMLMQQFGLNYLQA
                     LFIGTSIIVAYTFIGGFLAVSLTDFFQGTLMFLFLLIVPYMASKALGGWQPTLDMITQ
                     EGPEYLDPMNQFNGLLFVNLMAWGLGY"
     gene            979..1881
                     /locus_tag="VFP93_05045"
     CDS             979..1881
                     /locus_tag="VFP93_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9844004.1"
                     /translation="MIKPTENLKNRKSTASAKATSLGRMGVPFYCLFVAYCALIPFSN
                     WRVPPGNIFDTLVYGWFDKIFLFDIIQNLLLYIPLGFFAVFAFRNPRRSPWLALLAAF
                     IISCSMCSLLEFLQSFNPARVPSALDIMLNTLSGVFGGILALLFYNEWIKSLHFLSEE
                     VLFPISLRQPIPLLGLMIILAWGLYQWYPFLPSLHPEHLQYGFAPFKKVLHNFSLLNE
                     QKLCSYATQAVILYCCALLACKKNHFLFIFWYILIILSFKALIIGRVLSFEAFFGSFA
                     GLFIAGVLHQLLITLTRVFEKQQS"
BASE COUNT          632 a          381 c          362 g          669 t
ORIGIN      
        1 aatatcctaa tccccatgcc ataagattta caaataaaag accattaaat tggttcatag
       61 gatcaagata ctctgggcct tcctgcgtaa tcatatcgag tgttggctgc catccaccta
      121 acgctttaga agccatataa gggacgataa gtaaaaataa gaacattaaa gtaccttgga
      181 aaaaatcggt caaacttacc gctaaaaatc caccgataaa agtgtatgcc acgataatac
      241 ttgtaccaat gaataacgct tgtaaataat ttaaaccgaa ttgctgcatg agcattgctg
      301 cagccactaa acccgaagcc gtatagaaag caaaaaatgt taacgttatt aacgctaaag
      361 aaatacgaat gatgccagat ctatcgaaaa agcgattatc caagtaagcg ggtacggtta
      421 aggaatcttg cgcgatttca gaaaataaac gcaaaggttt tgctaagatg cgccaactaa
      481 tataggcacc tatggttagg ccaataggca accagatttg attgaggcca aaactaaata
      541 ctgcacccgg taaacccaat agaagccaag cactcatatc cgacgccccc gcacttaatg
      601 ctgcaaccgg gccacctaag cttctaccac ctagtacaaa atccgataag ttttttgtaa
      661 caaaatacgc tgttagagca ataattacga caactaggag ataagcacaa aatgcgataa
      721 tagttagaat gctcatattt ctttattttt taaaatttcg aacttaggta tttaacaaga
      781 aaaatcttat caaagcaatg tactcttaag tttgtcatga aactgcattt tattatgagg
      841 acatccacga ttttgagaaa atttaagagg acagtcataa gaagatttaa gaggacagtc
      901 ataataaaaa gaataaagac atccatgatt tgatactgtt gctatgcagt attcggagtt
      961 gtgctaaacc tttagtaaat gataaaacca actgaaaatc tgaaaaatag aaaatctacc
     1021 gcatccgcga aggctacaag cctcggacgc atgggggtac ctttttattg tttgtttgtg
     1081 gcttattgtg cactcattcc cttttcgaat tggagggtgc cgccgggcaa catattcgat
     1141 acgttagtat atggttggtt tgataaaatt tttctattcg atattattca aaacttactg
     1201 ttgtacattc cattaggttt ctttgcagta tttgcgtttc gaaatcctcg gcgttctccc
     1261 tggttagcgc tattggcggc atttattatt tcttgctcta tgtgtagtct tttagaattt
     1321 ttacaatctt ttaatccagc gcgtgttcct tccgcattgg atataatgtt aaacacactc
     1381 agtggcgttt ttggtggcat cttagcgcta ctgttttata acgagtggat caaaagttta
     1441 cattttctaa gcgaagaggt gttgtttccg atctctttaa ggcaacctat tccgctacta
     1501 ggtcttatga tcattttagc ttggggttta tatcaatggt accctttttt gccctcattg
     1561 catcctgagc atcttcaata tggttttgca ccttttaaaa aagttctgca taatttttct
     1621 ttactgaatg agcaaaaact ttgttcttat gcaacgcaag ctgtgatttt atattgttgt
     1681 gcacttttag cctgcaaaaa aaatcatttt ttgtttattt tttggtatat attaatcatt
     1741 ctatcgttta aagcattaat tattggaaga gttttatcgt ttgaagcgtt ttttggaagt
     1801 ttcgccggcc tttttattgc cggcgtattg catcaattac tgataaccct aacgcgtgtg
     1861 tttgaaaaac aacaatctta atccacgcaa ttccaattag gggaataacc atattgtttt
     1921 aatattttct gcaatacaac atttttttct ttatatacat gttgccatgc aattgcaaat
     1981 atttgccaat cttctttttt ctccgcatag tccaataatt gtttggtgtt atgtgttaac
     2041 agca
//
LOCUS       DASTIB010000293         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_797213, whole genome shotgun sequence.
ACCESSION   DASTIB010000293 DASTIB010000000
VERSION     DASTIB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797213"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFP93_05050"
     CDS             complement(<1..418)
                     /locus_tag="VFP93_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9844005.1"
                     /translation="MSKKPLLMVVEDDIGIREQLQWCFEEFEVIQAGDRHEAITKLKR
                     FDPAIITLDLGLPPDTLGVEEGMATLNEILRLKSTAKVIMVTGQSDRTYALQAINLGA
                     YDFYLKPIHKEELNFIANREYYLYQLEAENTKLEYCR"
     gene            complement(411..2498)
                     /gene="prsK"
                     /locus_tag="VFP93_05055"
     CDS             complement(411..2498)
                     /gene="prsK"
                     /locus_tag="VFP93_05055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HET9844006.1"
                     /translation="MTEVAVISHGMGAAAFFAIVLVLLFHPPTRQNSPWIILGCFFTA
                     IWSTLATLYVEQFITNYQWIALAEVFQDGSWCFALTKMLTSNEPDLRAPLLRKNFLKG
                     LLLTGLSLMLVNALLIHQQWLPGIKETSFVHFDFIGHLLLSILGLSLVEQLMRGIKPE
                     RLWGMKFFCLGIGTLFCYDFILYSHSLLFKNMDSTLWGVRGALNLFVAPMIVLSTRRT
                     IKWHLDIVPSRLLVYRSTAIIVCGIYLFTVGLIGYFLRELSGNWGKALQVLFIIASAL
                     FLFLLVFSGSARAWIKMFLARNVFKLYYDYREEWLRFNNLLSTPKQDTPLGARIIIAF
                     ANMVESPEGVLFERQGNSFRIKNTWNVNLEGSFTDAKFNKFLDSLREPLLMENFQSLV
                     QNYALEFPSQLGNYHRAWLFLPLHINNQCEAFIIIGQPRVTLKLNWEICDLLKTAASC
                     AAIFLYEQKLEEALHIAKQFEGYHRVSAFMLHDIKNVISSIQLILQNRKFANDVKFID
                     SLFITLENIHLKLSNLRKQLYNPNYESITPPFNIVSMLLDIVKEYQQLKKPVQFNPAS
                     FSKIVVMGNSEKLKDCIKHVIDNGLDACRNQTPVELVLHEMKAEVVLAIIDKGQGMDT
                     HFLQTKLFKPFVSTKGTRGMGIGVYQTKTYLESIGGKIFVESAEGKGTTFKLIIPKSA
                     DKVKEKLLEKSYE"
     gene            complement(2570..3955)
                     /locus_tag="VFP93_05060"
     CDS             complement(2570..3955)
                     /locus_tag="VFP93_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HET9844007.1"
                     /translation="MESIKSFFYCLSPAYIALAAVEIFVFFVSLYVGVSLELHNFYHL
                     LEYFEVLIPEASLFALVMFLSLAAAGLYQRRLKEGRSGMLLRLITGIIVGVLMISQIS
                     YTIPALDMGHTIWLSVIGIAFSSIFLLRMALFPRLEAIALRRRVLILGEGRVSKILQE
                     DLTSLRKPAYELVDILPLQILANPQRKRKNFDLLALAQQKKIDEIIVVLHAHDQTFNT
                     EPLLQCKFYGISVTDIVAFYERESGKIMLSCLFPSWFLLAKGFQRGAWVKAIKRCTDL
                     LFSILIFIISLPVILLVALAVWVEGGGKAPVVFKQKRVGENGKLFELLKFRSMRVDAE
                     KNGPQWASDNDARVTRVGRFIRKTRLDEFPQLFNVFKGEMSFVGPRPERPEFVIELAK
                     KIPFYHERHVIKPGITGWAQVCYSYGSSAQDSYEKLQYDLYYVKNYSLFLDFVILLQT
                     VEVLLLRRGAR"
     gene            complement(4059..>4307)
                     /locus_tag="VFP93_05065"
     CDS             complement(4059..>4307)
                     /locus_tag="VFP93_05065"
                     /inference="COORDINATES: protein motif:HMM:NF045154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844008.1"
                     /translation="PNLRHETLVLLPVERILLQYLDGKRKISKINRILEEKVLNKELN
                     LTDEQNNPIVEKKQIHERIQKLTLSTLENLARKAYLLA"
BASE COUNT         1414 a          835 c          706 g         1352 t
ORIGIN      
        1 gtctacaata ttctaacttg gtattttcgg cttccaattg ataaagataa tattcgcgat
       61 tcgctataaa atttaattct tccttatgta taggtttgag ataaaagtca taagcaccca
      121 aattaattgc ttgcaatgca taagttcgat cgctttgtcc ggttaccatg ataacttttg
      181 ccgtactttt taaacgcaag atttcattta aagtagccat tccttcttca acaccgaggg
      241 tgtcaggagg taatcctaaa tctaacgtga taattgcagg atcgaatctt ttaagttttg
      301 taatggcctc atggcgatcg ccagcttgaa taacttcaaa ttcttcaaaa caccattgaa
      361 gttgttcacg aataccaata tcatcttcca caaccattaa aagcggtttt ttactcatag
      421 cttttctcaa gtaatttctc ttttacctta tcagcggatt ttggaattat aagtttaaaa
      481 gtagtaccct ttccctctgc actttcaacg aaaattttac cgcctataga ttccagatac
      541 gttttagttt ggtaaactcc aatccccatg ccacgtgttc ctttggtaga aacaaatggt
      601 ttaaacaatt tggtttgtaa aaaatgggta tccatacctt gtcctttatc gattatggct
      661 aagacgactt ctgctttcat ttcgtgtaaa actaattcta ccggtgtttg attacgacaa
      721 gcatctaacc cattgtcgat cacatgttta atgcaatctt ttaacttttc actattgccc
      781 attacgacta tttttgaaaa actagctggg ttaaattgaa ccggcttttt aagttgctga
      841 tactctttaa cgatatccag caacatggat actatattaa aaggtggcgt tatactttca
      901 taattgggat tatacagttg tttgcgtaaa ttgcttaact taaggtgtat attttccaaa
      961 gtaataaaaa ggctatcaat aaattttaca tcgttagcaa attttctatt ttgtagaata
     1021 agctgtatag aacttattac attttttatg tcgtgtagca taaaagccga aactcgatga
     1081 tagccttcga attgtttcgc aatatgtaaa gcttcttcta atttttgttc gtataaaaat
     1141 attgccgcac aactcgctgc ggttttaaga agatcacaga tttcccagtt taattttaag
     1201 gtaaccctag gttgcccaat aataataaaa gcttcacatt ggttgttgat gtgcaagggc
     1261 agaaaaagcc atgcacgatg ataatttccc aattggcttg gaaattccaa agcataattt
     1321 tgaacgagtg attggaaatt ttccatcaat aatggttcac gcaagctatc taaaaattta
     1381 ttaaattttg catccgtaaa gctaccttct aaattaacat tccaggtatt ttttattcta
     1441 aaactatttc cttgacgctc aaacagtaca ccttccggac tttctaccat atttgcgaaa
     1501 gcaataataa ttcgtgcgcc taagggtgta tcttgtttcg gtgttgatag taaattattg
     1561 aaacgaagcc attcttcgcg ataatcataa taaagtttaa atacattacg cgccaaaaac
     1621 atttttatcc aagcgcgagc actacctgaa aaaacgagca aaaataaaaa caaggctgag
     1681 gcaataataa atagtacttg aagcgccttt ccccagttgc ctgaaagctc gcgcaaaaaa
     1741 tatccaatta atccgacggt aaataaatag atgccacaaa ctataatggc agtagatcga
     1801 tacacgagta ggcgagaagg cacgatgtcc agatgccatt taatggttcg tcgtgtggaa
     1861 agcactatca taggagcaac aaaaagattt aaagcacctc gcacacccca caaagtgcta
     1921 tccatattct tgaaaagaag cgaatgcgag taaaggatga aatcataaca aaatagagtg
     1981 cctatgccta aacaaaaaaa tttcatgccc catagtcgtt ctggtttgat accacgcatt
     2041 aattgttcca ctaagctcag tccaagaata ctgagtaata agtggccaat aaaatcaaaa
     2101 tgcacaaagg aagtttcttt aattccaggt aaccattgct gatggatcaa taaagcattg
     2161 acaagcatta aacttagacc cgttaacaat aatcctttga gaaaattttt gcgtaacaac
     2221 ggcgcgcgca aatcaggttc gttggatgta agcatcttcg ttaaagcaaa acaccaactt
     2281 ccgtcctgaa aaacttctgc taaagctatc cattgataat tggtaatgaa ttgttccaca
     2341 tataacgtgg ctaacgtgga ccaaatagca gtaaaaaagc agccgagtat gatccaggga
     2401 gaattttgac gtgtaggtgg atgaaaaagt aatactaata ctattgcaaa gaaggccgca
     2461 gctcccatac catggcttat aacagcgacc tcagtcatat cgtttccttt gatattcaaa
     2521 aataaattat actatgcgaa gaaaaagtat gcttttcttt attgaaattt tatctggcgc
     2581 cgcgtcgcag aagtaagact tctactgttt gcaataaaat aacaaagtct aagaatagac
     2641 tgtaattttt tacataatac agatcatatt gtaatttttc gtaagaatct tgcgctgaag
     2701 aaccgtaaga ataacacact tgagcccacc ctgtaattcc aggctttatt acgtgtcttt
     2761 catgataaaa tggaattttt tttgctaatt caatgacgaa ttcaggacgt tctggcctcg
     2821 ggcctacaaa actcatttcg cctttaaaaa cattgaataa ttgcgggaat tcgtccaatc
     2881 ttgttttgcg aatgaaccta ccaacacgcg ttacgcgcgc atcattatca cttgcccatt
     2941 gcggtccatt cttttcagca tctacgcgca tgctgcgaaa tttgagcaac tcaaagagtt
     3001 tcccgttttc accgactcgt ttttgtttga aaacgaccgg tgcttttcca cctccttcaa
     3061 cccaaacagc aagagctacc aacagaatca ctggcaaaga aataataaaa ataagtatac
     3121 taaaaagtag atcggtacat cgtttaattg cttttaccca agctcccctt tgaaagcctt
     3181 ttgctaagag aaaccaagag ggaaataaac aactcaacat gattttgcca ctttctcgtt
     3241 cgtaaaacgc tacgatatcg gtaacactaa ttccataaaa cttgcattgt aataagggct
     3301 ctgtattaaa tgtttgatcg tgtgcgtgta aaaccacaat aatttcatca attttttttt
     3361 gttgtgctag cgccaacaag tcaaaatttt ttctttttct ttgtgggttt gcaagaattt
     3421 gcaagggtaa aatatctact aattcataag ctggctttct taaagacgtt aaatcttctt
     3481 gcaaaatttt tgatactcta ccttctccta atattagaac tcttcgtcgt aacgcaatcg
     3541 cttctagtcg tgggaacaaa gccatgcgta ataaaaaaat cgaagaaaaa gctattccaa
     3601 tgacagataa ccaaatggta tggcccatgt ctaatgcagg aatcgtatat gaaatctgac
     3661 ttatcattaa tacaccgaca ataattccgg taattaagcg aagcagcatt ccacttcgcc
     3721 cctcttttaa acgtcgttgg tataagccag ctgccgccaa agataaaaac ataactaaag
     3781 cgaataatga agcttcgggt attaaaacct cgaaatactc taaaagatga taaaaattgt
     3841 gtaattctaa gctgacacca acgtataaag aaacgaaaaa tacgaatatc tcaacagcag
     3901 ccaatgctat ataggcaggc gataaacaat aaaaaaatga ttttatagat tccataaacc
     3961 accttccgtg gtagttactt atcataacta atagttatta gaaatgatat gcaataatac
     4021 tagtcagcca aattactttt accctaaaag ggtcctttct aagccaacaa ataagctttt
     4081 cgcgctaaat tttctaatgt cgataatgtc agtttttgta tacgttcgtg gatttgtttt
     4141 ttttctacaa taggattgtt ttgttcatct gttaaattta attctttatt caatactttc
     4201 tcctccaata ttctgttgat tttagaaatc tttctttttc catctaaata ttgtaaaagt
     4261 atacgttcta cgggcaacag cactaaagtt tcatgacgta aatttgg
//
LOCUS       DASTIB010000294         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_872101, whole genome shotgun sequence.
ACCESSION   DASTIB010000294 DASTIB010000000
VERSION     DASTIB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872101"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..142
                     /locus_tag="VFP93_05070"
                     /pseudo
     CDS             44..142
                     /locus_tag="VFP93_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025021.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            218..1012
                     /locus_tag="VFP93_05075"
     CDS             218..1012
                     /locus_tag="VFP93_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9844009.1"
                     /translation="MQNLPALHKAAKKGDLQKIEELLEANAIAINEQDQNGRTAMHYA
                     AKNGNLEVCKKLASAGIDIDAADFSGLTSLYSAVKNQDKELTEFLINSGSNVHFVYGS
                     ARNIMDRAKVAKKHTPFSKWLKNKISYPKDLLESLSQQMSLLISESLTLAKSQGKQLL
                     VMLGECHGEYPIQQAEDILLKIASNLGIKKLLIESDTILNNNLLEIKAREKLGFIITP
                     VDMHPERLLVTAPDWMFKRNVFMTDIINRTIMEDSVFITGSAWFAQ"
     gene            1002..1208
                     /locus_tag="VFP93_05080"
     CDS             1002..1208
                     /locus_tag="VFP93_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844010.1"
                     /translation="MLNEEGSTIDSHKFHVLPINLAALSIGASEITEEEKTPEDFFAD
                     DPNKVIQIDSNLNVSDAKKIFSKI"
     gene            complement(1262..2188)
                     /locus_tag="VFP93_05085"
     CDS             complement(1262..2188)
                     /locus_tag="VFP93_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9844011.1"
                     /translation="MSEIDLKKINLNLILALDALITEQNVTRAGEKLGLGQSAMSHSL
                     HQLRSILNDHILVRGQSSKMMLTPLAKTLGPKAKQITNEIKSLFAGSHPFLAAESKRV
                     YHVGMSDYLAMVLLPKLMQQITNEAKNIQIVVRHLNFLNNIAIFEKEECDLVLGNFDE
                     ASELLFKQKLFTDEAVCVAHKTHPAFKKALITLDEYAKYPHVLVGLEEDPGVSFIEKI
                     MKHKGYSIQIASIVPHTLTALYTLLPHTNFIASTVLRIAKEAKNYAELAIRPMPFQVG
                     KYVCSQYWHPKDNDDPGHRWLRVLVKKVSEDI"
     gene            complement(2281..>2463)
                     /locus_tag="VFP93_05090"
     CDS             complement(2281..>2463)
                     /locus_tag="VFP93_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844012.1"
                     /translation="CSFKDKTDSNTLEAMGEVPGTYFLRVSQKNYKALFVQLFFVNDP
                     FLAVCCVEDIFYIATA"
BASE COUNT          814 a          425 c          458 g          766 t
ORIGIN      
        1 cagctctttt gcctaaaaat ccgacccaaa ttggacagtt cttcatgctg ctgctgagtt
       61 gaatgatttg gacgaagtag agagaattct agaaaaatcg cctgaattgt taaacgttaa
      121 agataaaaat ggtcaaagtg catgtgatgt tgcgggtgtg tgcggaaatt tggaaaccgg
      181 tgtttatatg ataaattttc ctggcattta tcaatccatg caaaatttac cggctctaca
      241 taaagcagct aaaaagggag atttgcaaaa aattgaagag ttattagaag caaacgcaat
      301 tgccataaat gaacaagacc aaaatggaag aacggcaatg cattatgcag caaaaaatgg
      361 aaatcttgag gtgtgcaaaa aattagcttc ggcaggcata gatatagatg cagctgattt
      421 cagtgggctt acttctttat atagtgcagt taaaaatcag gataaagaac ttacggagtt
      481 tttaataaat agcgggagta acgtacactt cgtatacgga agtgctagaa atattatgga
      541 tcgcgcgaaa gttgcaaaaa aacatacccc tttttcaaaa tggttaaaaa ataaaataag
      601 ttatccaaaa gatttattgg aatccttatc acaacagatg tcacttctta tttccgaatc
      661 attaacttta gcaaaatcac aaggaaaaca attacttgtt atgcttggag aatgtcatgg
      721 agagtatccc atacaacaag ctgaagatat tcttttaaaa attgcttcta atttaggaat
      781 taaaaaactt ctcatcgaat cagatacgat actcaacaat aatttactcg aaatcaaagc
      841 acgtgaaaaa cttggattta tcattacacc ggttgatatg catccagaac gtcttttggt
      901 tacggctcca gattggatgt ttaaaagaaa tgtatttatg acagacatca ttaatcgcac
      961 tataatggaa gatagtgttt ttataacagg atctgcttgg tttgctcaat gaagaaggta
     1021 gcacaattga ttcgcataaa tttcatgtat tacccatcaa tttagcggca ttatccatag
     1081 gggcctcaga aataacagaa gaagaaaaaa ctcctgaaga tttttttgcg gatgatccaa
     1141 ataaggtaat tcaaatagac tctaatttaa atgtaagtga tgcaaaaaag attttttcaa
     1201 agatttaaaa tatactatga agttattttg ccaagagcag aataacttct ccatgaatgg
     1261 atcaaatatc ttcgcttact ttcttgacta aaactcgaag ccatcgatga ccaggatcat
     1321 cattatcttt tgggtgccaa tattgggaac aaacatattt acctacttgg aatggcatag
     1381 gcctaattgc caattcagcg taatttttag cctcttttgc gatgcgcaaa actgtggaag
     1441 caataaagtt cgtatgcggt aatagcgtat agagcgcagt taatgtgtgc ggtacgatgg
     1501 atgcaatttg tatagaatat cctttatgtt tcataatttt ttctataaag cttacgcctg
     1561 gatcttcttc aagccccact aacacatgag gatacttggc atattcatct aatgtaatca
     1621 aagccttttt aaaagccgga tgtgtcttgt gcgccacaca tacggcctca tctgtgaata
     1681 acttctgctt gaatagtaat tctgatgctt cgtcaaaatt tcctaataca agatcgcatt
     1741 cctccttctc aaatatagct atattgttaa ggaagtttaa atgtctgacc actatttgta
     1801 tattttttgc ttcgttcgtt atttgttgca ttaatttggg aagtaaaacc atggctaaat
     1861 aatccgacat tcccacatgg taaacgcgtt ttgattctgc cgctagaaaa ggatgtgatc
     1921 ccgcgaataa tgacttaatt tcattcgtaa tttgttttgc ttttgggcct agggtttttg
     1981 ctaaaggggt gagcatcatt ttacttgatt ggccacgtac caatatgtga tcgttgagaa
     2041 tggatcttaa ttggtgcagg gaatggctca ttgccgactg tcctaaccct aacttttctc
     2101 ccgcgcgggt cacattctgc tccgttatta gagcatcaag cgccagaatt aaatttagat
     2161 ttatcttttt taaatcgatt tcactcatat atattattat tataatcgat tgaaataatg
     2221 aattaaagca gttaatctat acatattcca ggtagataac aaacttttta ggagaaatca
     2281 tcatgctgtg gcaatataaa atatatcttc aacacaacat acagctaaga agggatcgtt
     2341 tacgaaaaat agctgaacaa acagagcctt gtaattcttt tggcttacac gtaaaaagta
     2401 ggttccaggc acctcgccca tggcctccag ggtattggaa tccgttttgt ccttaaaaga
     2461 gca
//
LOCUS       DASTIB010000295         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1245377, whole genome shotgun sequence.
ACCESSION   DASTIB010000295 DASTIB010000000
VERSION     DASTIB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245377"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..820
                     /gene="atpB"
                     /locus_tag="VFP93_05095"
     CDS             53..820
                     /gene="atpB"
                     /locus_tag="VFP93_05095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9844013.1"
                     /translation="MIEAHPVTQTEYINHHLTNLHFGEGFWSIHLDTLIISWILGLGF
                     LFLFHYAAKRATSGVPGALQNFVEVIYDFVDRQVKDTFHGKSDFIAPLGMTIFVWVFL
                     MNLMDLLPVDLLPVIGSYMGLSHLKVVPTTDPNLTLGMSLTVFLLTIFYSIKWKGLGL
                     LKEIFTQPFGPWLFPFNFLLRMVDECAKPLSLGLRLFGNLYAGELIFILIALLPWFIQ
                     WPLGWTWAVFHILIITLQAFIFMMLTIVYISQAHEAH"
     gene            856..1140
                     /gene="atpE"
                     /locus_tag="VFP93_05100"
     CDS             856..1140
                     /gene="atpE"
                     /locus_tag="VFP93_05100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET9844014.1"
                     /translation="MDTASLVSQLNGFTAVAVALLIGLGALGTAVGFGLLGGKFLEGA
                     ARQPEMVPMLQVKMFIVAGLLDAVSMIGVGIALYFTFASPFLAELKNYIQ"
     gene            1155..1625
                     /locus_tag="VFP93_05105"
     CDS             1155..1625
                     /locus_tag="VFP93_05105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET9844015.1"
                     /translation="MNINATLLGQMITFLLFVLFTKRYVWPYLQNSLRERQEKIAEGL
                     AAAERGHHDLVLAQEAAVKKIKEGKEEASRIIEEAKKQATHLIEIAKQNAITEADRLR
                     GKAQVDIEHMVTQAQETLRRQAAKIAVLGAEKILARQIDEAANQELLEDLVREI"
     gene            1633..2172
                     /locus_tag="VFP93_05110"
     CDS             1633..2172
                     /locus_tag="VFP93_05110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET9844016.1"
                     /translation="MSKKYTIARPYAKAAFSLAQTSSQYEAWSQMLQLGALMAQDPAF
                     VELLKDPKFPDAMALEWLLSIGKELITKEGEQFFNLLSDNQRLLFLPEIAELYEELKL
                     EAEKVIKASVISAQPLDENNKARIREALQSRFHASEIELDCKVDKNLLAGAIVRAGDL
                     VIDGTARGRLRRIEEALGI"
     gene            2195..>2970
                     /locus_tag="VFP93_05115"
     CDS             2195..>2970
                     /locus_tag="VFP93_05115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639836.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9844017.1"
                     /translation="MRLNPSEISELIKKRIEHSEIKAQSRTVGTIISSGDGIVKIHGL
                     KDVMQGEMLKFPGDTYGMALNLERDSVGAVVLGAYEHLMEGQTVECTGRILEVPVGHA
                     LLGRVVNALGEPIDGKGEIKYETTSPIEKVAPGVITRKSVSQPVQTGLKAIDSMVPIG
                     RGQRELIIGDRQTGKTAIAIDAIINQKDTGIKCIYVAIGQKASSVASVVRKLEEHGAL
                     AHTIIVVASAAESAALQFIAPYSGCSMGEFFRDRGEDALI"
BASE COUNT          822 a          537 c          716 g          895 t
ORIGIN      
        1 gattgtcccc attttttggg ggtttcgtag gcaataaaaa gtgtgctgaa ttatgattga
       61 ggctcatcct gtgacacaaa cagaatacat caaccaccat ttaaccaact tgcattttgg
      121 tgagggtttt tggtctattc atcttgacac cttaattatt tcctggattt taggtttagg
      181 ttttcttttt ttgtttcact atgctgcaaa acgggcaaca tccggtgtgc ctggtgcttt
      241 acaaaatttt gttgaggtca tttatgattt tgttgaccgc caagtaaaag atacttttca
      301 tggaaaaagc gactttatag cgccattggg gatgactata ttcgtttggg tttttcttat
      361 gaatttgatg gatctgttgc cggttgattt gttgcctgta atcggcagct acatggggtt
      421 gtcacattta aaggttgtgc ctaccaccga tcccaacctt accttgggga tgtcacttac
      481 ggtatttctt ttaaccattt tttacagtat caagtggaag ggattgggac tgctcaaaga
      541 aatatttaca caaccttttg ggccgtggtt gtttcctttt aattttttat taagaatggt
      601 cgatgaatgt gccaagcctt tgtcgcttgg gttgcgatta tttggcaact tatatgcggg
      661 cgagctcatt ttcatattga tcgctttgtt gccgtggttt attcaatggc ccttaggctg
      721 gacttgggcg gtgtttcata ttcttattat aactttgcaa gcatttattt ttatgatgct
      781 tacgattgta tatataagcc aagcacatga agcacattaa gtttttgttc aattggtaaa
      841 agatgaggag taacgatgga tacagctagt cttgtttcac agcttaatgg ctttacagcc
      901 gttgcggtgg cgttattaat tggtcttggt gctttgggca ctgccgtcgg gtttggactt
      961 ttgggcggta agtttttgga aggcgctgcg cgccagcctg aaatggttcc gatgttgcaa
     1021 gtcaaaatgt ttattgttgc aggcctatta gacgctgttt ctatgattgg tgtgggtatt
     1081 gccctctact ttacttttgc aagtccgttc cttgctgagc taaaaaatta tattcaataa
     1141 tttggagagg ctttgtgaat atcaatgcca cccttttggg acaaatgatc acctttttgt
     1201 tgtttgtttt atttacaaaa cgttatgttt ggccttatct gcaaaattct ctaagagaac
     1261 gccaagagaa aattgcagaa ggtttggcag cagcagaacg gggacatcat gatttggtgc
     1321 ttgcccaaga agcagccgtt aaaaaaatta aggaaggcaa agaagaagcg tcacgcatta
     1381 tagaagaggc gaaaaagcag gcaacacacc tgattgagat cgccaagcaa aatgcaataa
     1441 cagaagcgga tcgtctcaga ggaaaagcgc aagtagatat tgagcatatg gttacacaag
     1501 cccaagaaac gttgcgtcgg caagcagcga aaattgcggt gctgggagcg gagaaaatat
     1561 tggcacgaca aattgatgaa gcagctaatc aagagttgct agaagatttg gttcgcgaaa
     1621 tatagaggac tcatgtcaaa aaagtacacc attgcaagac cctacgcgaa agctgcattc
     1681 tctttggcac aaacttcgag ccagtatgaa gcttggtctc agatgctaca attaggcgct
     1741 cttatggctc aagatcctgc gtttgttgaa ctgttaaaag atcctaaatt tccggatgca
     1801 atggcgcttg aatggcttct ttctattggt aaagagttaa tcacaaagga aggagagcag
     1861 ttctttaatc tcttatccga taatcaacgc ttgttgtttc ttcctgaaat tgcagagtta
     1921 tacgaagaat taaagcttga agctgaaaaa gtaataaagg cgagtgttat ttctgctcaa
     1981 ccgctggatg aaaataataa agctcggatt cgtgaagcgc tccaaagccg tttccatgcc
     2041 agtgagatag agcttgattg taaagtagat aaaaatttac tagcgggtgc cattgtgcgt
     2101 gcaggcgatc tagttattga tggcactgcg agaggtagat tgcgacgtat tgaagaggcc
     2161 ctagggattt aaataataga agtgagattt agacatgcga ttaaatccat ccgaaatcag
     2221 cgaactcatt aaaaaacgga ttgaacattc tgagattaaa gcccaatctc gtaccgtggg
     2281 cactattatt agttcgggcg atggtatcgt taaaattcat ggcttaaaag atgttatgca
     2341 aggggagatg ttaaagttcc ccggcgatac ctatggtatg gcgctgaatt tagaacgcga
     2401 ttctgtaggt gctgtggttc taggagctta tgagcacctc atggaaggtc aaacggttga
     2461 atgtacgggc agaattctgg aagttcccgt gggtcacgcg ttgttaggtc gtgtggtaaa
     2521 tgccttaggt gaacccatag atggtaaagg ggaaatcaaa tatgaaacta cctcccccat
     2581 tgaaaaagta gcaccgggcg ttattacgag aaaatcggta agccagcctg tgcaaactgg
     2641 tttaaaagca attgactcca tggtacccat tggccgtggt cagcgcgaat taattattgg
     2701 cgatcgtcaa accggtaaaa ccgcgatagc gattgacgct attatcaacc aaaaagacac
     2761 tggcataaaa tgtatttatg tcgctatcgg tcaaaaagca tcttcagttg cctcagtagt
     2821 acgtaaattg gaagagcatg gagcgttagc ccataccatt attgtggtcg catctgcggc
     2881 agaatcggca gcattacaat ttattgcgcc ttattcgggt tgctcgatgg gagagttttt
     2941 tcgcgatcgc ggtgaagatg cccttataat
//
LOCUS       DASTIB010000296         4270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1200002, whole genome shotgun sequence.
ACCESSION   DASTIB010000296 DASTIB010000000
VERSION     DASTIB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200002"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..734)
                     /locus_tag="VFP93_05120"
     CDS             complement(21..734)
                     /locus_tag="VFP93_05120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET9844018.1"
                     /translation="MPYLKIFDQFVPFQLRRSARSTRLRLEWNGGDAKVIAPTKVDNQ
                     QILHFLFHHKPWLEKQIRKRRYKSAELHAMHWPSFYASGTELPYVGVRVRLHICYSEK
                     VRVQRLKRQLFIYSPKIDQSTIKIIVKQWFYKQALMHANHWLRYYRNRLQRAPRSLTI
                     KKTKTLWGSCGIHGDIYLNWQLVCMPLEIFHYVFLHEMCHLFYRGHGKKFWGMVRKWM
                     PDYKEKEKRLRHYAPLENF"
     gene            834..1679
                     /locus_tag="VFP93_05125"
     CDS             834..1679
                     /locus_tag="VFP93_05125"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HET9844019.1"
                     /translation="MKSGPYNCKYFRPFLKWPGGKYRLLQHLQPQFPQASLLIEPFVG
                     AGAIFLNTDYANNWLNDINADLIGLYMHLQKEGEIFIKEAKKFFSQKNNTPGRYYLLR
                     DHFNQSNDTYEKALLFLFLNRFGFNGLCRYNNKGIYNVPFGKYKKPYFPEIELFRFLE
                     RSKQLQFFNVDYQEMMQKAANHTKEAVIYCDPPYVPLSKTANFTQYSGKIFDFTDQIN
                     LARLSEQLAKQGKTVIISNHDLPATRLLYQKAQIFSIKVSRVISAIAKKRILVPELIA
                     VFHRH"
     gene            complement(1653..2336)
                     /locus_tag="VFP93_05130"
     CDS             complement(1653..2336)
                     /locus_tag="VFP93_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HET9844020.1"
                     /translation="MRKFIWGIILLCSCFYVFASTDFQEGKHYQQVPIEAFQNELVQE
                     LKAKYPKKILVVEFFSYGCHWCHELEPHLKTWLETHKADYVEFVRIPVVFQSSWLNLS
                     KAYYSAEQLGILDKFHAAMFTAIHEEKLQSSTQEALASFFETQGVAKKDFLKVFRSFG
                     VEQQAKQANQLSMIFKITAIPTFVIQGENNALITSTRMVGGEAQVFDLISALVKEEHR
                     VLMSVENSN"
     gene            complement(2347..3132)
                     /locus_tag="VFP93_05135"
     CDS             complement(2347..3132)
                     /locus_tag="VFP93_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9844021.1"
                     /translation="MKSSWIVVLLFILHTPLFAANASNGAQTGDTNASATQPNNTNVS
                     EEAQKNSVEEPNPSTNTSAESTNGTQGEIETTQSSQTHAQGFDKASTCVACHGPDGNS
                     VVPMWPKIAGQHLPYLVKQLKDYRLGENGPRFDPQMYSMVMQLTDEDILQLAQYYAGL
                     KQTPGKALADYLELGQRIYRGGLLEKGISACTACHGPNGMGNDLAGFPRLSGQHAAYT
                     ESQLKRFKDGQRKNDKNSIMRDIAVRMSDEEMKAVSEYIAGLH"
     gene            3315..3947
                     /gene="yihA"
                     /locus_tag="VFP93_05140"
     CDS             3315..3947
                     /gene="yihA"
                     /locus_tag="VFP93_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET9844022.1"
                     /translation="MTQVPMVPLVYKLNLNNTTFLQSAPNLLTSPPDKGIEVAFVGRS
                     NAGKSSAINAIVGKNGLARTSKTPGRTQVMNYFTIDDDRRLVDLPGYGYAKVPTRTKQ
                     QIEQLLSEYLSDRQCLHGFFMLMDVRLPLTSYDKQLIDFAAQTGKLIHILLTKCDKIK
                     RGAANSTLLSVKKSLSTYEDQLSIQTFSALKNLGIEQAREVLSDWFNFDE"
     gene            complement(4114..4269)
                     /locus_tag="VFP93_05145"
     CDS             complement(4114..4269)
                     /locus_tag="VFP93_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET9844023.1"
                     /translation="MQVHDELVFELSDTILEIALGTIKELMESAAILSVPLEVSVGYG
                     KNWQEAH"
BASE COUNT         1280 a          873 c          785 g         1332 t
ORIGIN      
        1 tgtttgcacc ttttattgca tcaaaaattt tccagtggag catagtgacg caatcgtttt
       61 tctttttctt tgtagtccgg catccatttt ctcaccattc cccaaaattt ttttccatgc
      121 cctcgataaa acaaatgaca catctcatgt aaaaaaacat agtgaaaaat ttctaaaggc
      181 atgcaaacga gttgccagtt taaataaata tccccatgta tgccacaact accccacagt
      241 gttttcgttt ttttaatcgt taaggatcgg ggagcacgtt gcaaacgatt acggtaataa
      301 cgcaaccagt gattggcatg cattaaagct tgcttataaa accattgctt aacaataatc
      361 ttaatagtag attgatcgat tttgggacta tagataaaaa gttgtcgctt taaccgttgc
      421 acccgaactt tttccgaata gcaaatgtgc aaacgtacgc gtactcctac ataaggaagc
      481 tccgttcccg aagcataaaa cgatggccaa tgcatagcat gcaattctgc acttttgtag
      541 cgtcgtttgc gaatctgctt ttcaagccat ggcttgtgat ggaaaagaaa atgtaatatt
      601 tgttggttat caacctttgt gggagcgata acttttgcat cccctccatt ccactctagt
      661 cgcaacctag tagatcgcgc tgatctgcgt aactgaaatg gcacaaattg gtcgaaaatt
      721 ttcaagtagg gcatattgtt acttaactaa acgcatataa atttacttgg cgctatgcac
      781 gtaacattta taatagggga tatttccttt ttaagttact gcatttgact gttgtgaaaa
      841 gtggccccta caattgtaaa tatttccgtc cttttttaaa atggcctggc ggtaaatatc
      901 gactattgca acacttacaa ccccaattcc ctcaagcttc attgcttatt gagccttttg
      961 tgggcgctgg cgcaattttt ttaaacaccg attatgccaa caattggctg aatgatatta
     1021 atgccgattt aatcgggtta tatatgcatt tacaaaaaga aggtgaaatt tttataaaag
     1081 aagccaaaaa attttttagc caaaaaaaca atacccctgg ccgttattat ttattacgcg
     1141 atcactttaa tcaaagcaac gatacctacg aaaaagcact attgtttcta tttttaaatc
     1201 gttttggatt taatgggctt tgtcgctaca acaataaggg tatttataat gtgccctttg
     1261 gaaaatataa aaaaccgtat tttccggaaa tagagttgtt taggttttta gaaagatcaa
     1321 aacagttaca attctttaat gtggattatc aagaaatgat gcaaaaagct gcaaaccata
     1381 cgaaagaagc tgttatttat tgtgatccac cttatgtccc tttaagtaaa acggcaaatt
     1441 tcacacaata tagtggcaaa atctttgatt ttaccgatca aataaattta gcccgcttgt
     1501 ctgagcaatt agctaagcaa gggaaaacgg tgatcattag caatcatgat ctaccagcaa
     1561 cccgcctttt atatcaaaaa gcacaaattt tttctattaa agtttcgcgt gttattagtg
     1621 ctatcgcaaa aaaaagaata cttgtgccag aattaattgc tgttttccac agacattaat
     1681 acacgatgct cctctttcac caaagcgctt attaaatcaa atacttgtgc ttccccccct
     1741 accatgcgcg tggaagttat taaagcatta ttctctcctt ggattacgaa agtaggaatg
     1801 gccgtaattt taaagatcat ggacaattga ttggcttgtt ttgcctgctg ctcgacgcca
     1861 aatgaacgaa acacttttaa aaaatctttt ttagcaacac cttgagtctc aaaaaaagaa
     1921 gctaaagcct cctgcgtact agattgtaat ttttcctcgt gaatggcagt aaacattgca
     1981 gcatgaaatt tatcaagaat tcccaattgt tcggcagaat aataggcttt cgataagttg
     2041 agccaagaac tttgaaaaac aacgggtatg cgaacaaact cgacgtaatc ggctttgtgt
     2101 gtttcgagcc atgttttaag atggggctct aattcatgac accagtggca tccgtaacta
     2161 aaaaattcaa ccacaagaat ttttttggga tatttcgctt ttaattcttg aactaattca
     2221 ttttgaaatg cttcaatggg aacttgttga taatgtttgc cttcttgaaa gtcggtagat
     2281 gcaaaaacat aaaagcagct acacagtaaa attattcccc aaataaattt tctcatactt
     2341 agtcctctag tgcaggccgg caatatattc acttacggct ttcatttctt catcactcat
     2401 acgtacagct atatcacgca ttatggaatt tttgtcgttt ttacgttggc cgtctttaaa
     2461 acgtttaagt tggctttcgg tgtaagctgc atgctggccg cttaagcgtg gaaatcccgc
     2521 taaatcattg cccataccgt tagggccatg gcaagctgtg cacgcgctta ttcctttttc
     2581 cagtaagccc ccgcgataaa ttcgttgccc taactctaaa taatctgcta atgccttgcc
     2641 aggggtttgc ttaagaccag catagtactg tgccaattgc aaaatatcct catcggtaag
     2701 ttgcattacc atgctataca tttgtggatc aaagcgagga ccattttcac cgagacgata
     2761 atcttttaac tgctttacca aatagggtaa gtgttgacca gcgatttttg gccacatggg
     2821 caccacactg ttaccatctg gaccatgaca agcaacacag gtagacgctt tgtcaaaacc
     2881 ttgagcatgc gtttgcgaag attgggtagt ctcaatctcc ccttgggtac cgttagtcga
     2941 ctccgctgat gtatttgtgg atggattggg ctcttcaacg gaattttttt gtgcttcctc
     3001 ggatacattt gtgttgttgg gttgtgtagc agaagcgttt gtatcaccag tctgcgcgcc
     3061 attgctagca tttgctgcaa ataacggtgt gtgtaaaata aaaagtaaga ctactatcca
     3121 gctcgatttc atgataaact tccaagaatt tttgttattg ttataactgc tgcgcgttta
     3181 ggcacgttgt tataaaacta gtcccacact ttagctccat gctagactga ggttatcaat
     3241 taaagactgc caacttatac cagcttgtct tgtaaatggt caacagttag cagatctgca
     3301 cttaaggtga gaacatgact caagttccaa tggtcccact cgtctataaa ttaaatttaa
     3361 ataacaccac ttttttgcaa agtgcaccta acttattgac cagtccaccc gataaaggta
     3421 tcgaggtagc atttgttggt cgctcaaatg caggtaagtc tagtgctatt aatgcaattg
     3481 tcggcaaaaa cggtttagca agaaccagca aaacaccggg tcgcacccaa gtcatgaatt
     3541 attttaccat cgacgatgac agaagattag tcgacttgcc aggctatggc tatgcgaagg
     3601 taccaacacg cactaagcaa caaatagaac agctgttaag tgagtattta agcgatcgtc
     3661 aatgtttaca tggttttttt atgcttatgg atgttcgact accgttaaca tcctatgata
     3721 aacaactcat tgattttgcg gctcaaacgg gcaagcttat tcatatactc ttaaccaagt
     3781 gcgacaaaat taagcgaggc gctgcaaact ccaccttact atccgtcaag aaaagtttaa
     3841 gcacgtatga agatcaactc tctattcaaa ccttttctgc tttgaaaaat ttaggtattg
     3901 aacaagcccg cgaagtactt tctgattggt ttaactttga tgaataaata aaaaacctgg
     3961 ctgtagaaag ccagggttaa cgtgcccgat cctgggggaa gatcgagcgt ttgccgtcca
     4021 tggtatactt tcaaattttc gtcaatttaa tgactttcca gggctttttt aagttcccca
     4081 aaagtaaaat atttgatcct tgctaaaatc tatttaatgc gcttcctgcc aatttttacc
     4141 gtatcccacg ctcacttcta gagggacact taaaattgca gcggattcca ttaattcttt
     4201 gatcgtaccc aatgcaattt ccaatattgt gtctgaaagt tcaaatacga gttcatcgtg
     4261 cacttgcatg
//
LOCUS       DASTIB010000297         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_267726, whole genome shotgun sequence.
ACCESSION   DASTIB010000297 DASTIB010000000
VERSION     DASTIB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267726"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="groEL"
                     /locus_tag="VFP93_05150"
     CDS             <1..1009
                     /gene="groEL"
                     /locus_tag="VFP93_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9844024.1"
                     /translation="NPYILLVDKKISNIREMLPILENVAKAGRPLFIVAEDVEGEALA
                     TLVVNNIRGIVKVCAVKAPGFGDRRKAMLQDIGVLTKANVISEEIGITLEKAALPDLG
                     EAKRVVISKENSIIIDGVGESKEIKARVDSIRKQVEETTSDYDREKLQERVAKLSGGV
                     AVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVPGGGVAFVRAIDAVKKMKVDNH
                     DQELGVNIIARALQAPLRQIVANAGGEPSVILNRVAESKGNFGYNAATGEYGDMLQMG
                     ILDPTKVSRSALQNAASVAGLMITTDCMIADLPKEEQNEAPSDMGMGGMGGMGGMGGM
                     M"
     gene            complement(1059..1697)
                     /gene="coq7"
                     /locus_tag="VFP93_05155"
     CDS             complement(1059..1697)
                     /gene="coq7"
                     /locus_tag="VFP93_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HET9844025.1"
                     /translation="MRKYSIADKVILGLQQFLHSTLPTSNPSARPNPSTLIEQDDLSF
                     EHRGLSQRLMRINHRGEVCAQALYQGQALAARDKTLRCNLLQAAQEETDHLLWCQTRI
                     RELQGRTSYLNTLWWAGSYSIGIIAGFVGDRWSLGFLAETENQVYTHLQKHLQQLPQE
                     DYKSRAILEQMQWDEQQHAISAIKQGAYDLPQPIVWMMSSTARIMTTLTYYA"
     gene            1767..2024
                     /locus_tag="VFP93_05160"
     CDS             1767..2024
                     /locus_tag="VFP93_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HET9844026.1"
                     /translation="MRFNIKKLVSGAISTALAVGVISAPSQVMGAEMEKCYGVAKAGK
                     NDCSAKGHACAAQAQSDGDPEEWVYVPKGTCEKIVNGVVKK"
     gene            2024..2893
                     /locus_tag="VFP93_05165"
     CDS             2024..2893
                     /locus_tag="VFP93_05165"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9844027.1"
                     /translation="MDSVKVTSKIVGGAQNRAISSIAGVGLGFRQEHFPLISQQLPKI
                     PWFEILTENFIQQSISQRKTLDFLRAHYPIALHGVSLSIGSTDPINFTYLRALKALIT
                     RYEPCWVSDHLSWVSLADHYVPDLLPLPFTHESLQHIISRIHRVQDYLQRPILLENIS
                     AYIQFEASSIPEWEFLELLCKATRCFLVLDINNIYVNAKNHTLCVKDFLNKITPSYVQ
                     EFHLAGFEENNTLLIDTHGAPIHTEVWELYTFALQRFGNKPTLIEWDTNIPEFSVLDQ
                     ERRKAEILQCQNV"
     gene            2878..3648
                     /locus_tag="VFP93_05170"
     CDS             2878..3648
                     /locus_tag="VFP93_05170"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9844028.1"
                     /translation="MSECLAKIQRQWMDLVNLGKNCSKKSMQGIIAPNPQAINVYRNT
                     IRISQLRCLEQTYPVCVQLVGNTYFTFLFEQYRIAYPQRNFDLHHYGHTFAAFLKDFE
                     LPHNILYLGDVAQLEWAIHHAEMCASEPQAVLDKLARIDPEVLDTLQFQLIHSATLLE
                     SAYPIKKIWELNQHAIVEEMIALDQESMKIIVWHNGQAVHFESLQDAEWDFLRSLQKN
                     FTLEQIFDHLHYVYRLQECEAQTMLAKFLHMNCLHISY"
BASE COUNT         1148 a          698 c          788 g         1180 t
ORIGIN      
        1 aaatccgtat attttgttag tagacaagaa gatttctaac attcgtgaaa tgttacccat
       61 cttagaaaac gttgctaaag caggtcgtcc attatttatt gtcgctgaag acgtagaagg
      121 cgaagcgtta gcaaccttag ttgtaaacaa tattcgtggt atcgtcaaag tatgtgcggt
      181 taaagcacct ggctttgggg atcgtcgcaa agctatgttg caagatatcg gagttttaac
      241 taaggctaat gtcatttctg aagaaattgg tatcacctta gaaaaagcag ctcttccaga
      301 tttgggtgaa gcaaaacgcg tagttatcag caaagaaaac tctatcatca ttgacggagt
      361 tggcgaaagc aaagaaatta aagcgcgtgt tgattctatc cgtaaacaag tcgaagaaac
      421 tacttccgac tacgatcgtg aaaaattgca agaacgtgtt gctaaacttt ctggtggtgt
      481 tgcggttatt aaagtaggtg ctgcaaccga agtggaaatg aaagagaaaa aagcacgtgt
      541 agaagacgct ttgcatgcaa cgcgtgctgc agttgaagaa ggtgtggttc ctggtggtgg
      601 cgttgctttc gttcgtgcta tcgatgcagt taaaaagatg aaagttgaca accatgatca
      661 agaattaggt gttaacatca ttgcacgtgc attgcaagct cctttacgtc aaatcgttgc
      721 caacgcgggt ggtgaacctt ctgttatatt aaaccgtgtt gcagaatcca aaggaaactt
      781 tggttataac gcagcaacgg gtgaatatgg cgacatgtta caaatgggta ttctggatcc
      841 aactaaggta tcccgtagtg ctcttcaaaa cgcagcttct gttgctggct taatgattac
      901 taccgactgc atgattgctg atctaccgaa ggaagaacaa aatgaagctc cttcagatat
      961 gggcatgggc ggtatgggtg gtatgggcgg catgggcggc atgatgtaat ttgtccctga
     1021 cccaaactct ctctaaaacc ccgcaattgc ggggtttttt atgcgtagta cgtgagtgtc
     1081 gtcataattc ttgcagtact cgacatcatc caaacaatgg gttgtggtaa atcgtatgca
     1141 ccctgtttta ttgcagaaat agcatgttgc tgttcgtccc attgcatttg ttctaatatg
     1201 gctctacttt tataatcttc ttgtggcaat tgctgtaaat gtttttgcaa atgggtataa
     1261 acctgattct cagtttctgc taaaaatccc aagctccatc tatcgccaac aaatcctgca
     1321 ataatgccta tggaatacga tccagcccac caaagcgtat ttaaataact cgtacgacct
     1381 tggagttccc ttattctagt ttgacaccaa agtaaatgat cggtttcttc ttgcgccgct
     1441 tgtagcaaat tacaacgtaa agttttatca cgagcagcta gtgcttggcc ttgataaagg
     1501 gcttgagcac acacttctcc cctatggtta atacgcatta accgttgact caagccacgg
     1561 tgttcgaaac ttaaatcatc ttgttctatt aaagtcgaag gatttggacg cgctgaaggg
     1621 tttgaggttg gtagagtaga atgaagaaat tgttgtaacc ctagtatgac tttatctgct
     1681 atagaatatt ttcgcaccac ttttaaaatt ttaccgattt taagctatga tagcctcatg
     1741 ttaatatact aataatggag atccaaatgc gttttaatat taaaaaatta gttagtggcg
     1801 ctatttctac tgctttagcc gttggagtta taagtgctcc aagccaagtt atgggggctg
     1861 agatggaaaa atgttatggc gtagctaagg ccggtaagaa cgattgtagt gctaaaggac
     1921 atgcatgtgc agcgcaagcg caaagtgatg gtgatccaga agaatgggtt tatgtaccga
     1981 aaggaacttg cgaaaaaatt gttaatggtg ttgtcaagaa atagtggatt ccgtaaaagt
     2041 tacctccaaa attgttggag gtgctcaaaa ccgcgcaatt tcttctatag cgggagtcgg
     2101 gcttggtttt cgccaagagc attttccctt aatttctcaa caattgccca aaataccctg
     2161 gtttgaaatt ttaaccgaaa actttattca acaaagtatt tcccaacgca aaactttgga
     2221 ttttttacgc gcgcattatc ctatagcatt gcatggggtt agtttatcga ttggatctac
     2281 cgatcctatt aattttactt atttgcgtgc attaaaagca ttgattaccc gctatgagcc
     2341 ttgttgggtg tccgatcatt tgtcatgggt atcgcttgca gatcattatg tgcctgattt
     2401 gctgccttta ccgtttacgc atgagtcgtt gcagcatatt atatctcgta ttcacagggt
     2461 tcaagactat ttgcagcggc caatactttt ggaaaatatt tcagcttata ttcagtttga
     2521 agcttctagc attccagaat gggaattttt agagttattg tgcaaagcta ccagatgttt
     2581 tcttgtgctc gacattaata atatttatgt caacgcaaaa aatcatacat tgtgtgtgaa
     2641 agactttttg aataagatta cacctagtta tgtgcaagaa ttccatcttg ctggatttga
     2701 agagaataat acactgctca tcgataccca cggtgcgcca attcatacag aagtttggga
     2761 attatatact tttgcattgc aaagatttgg taataaaccg actttgatag aatgggacac
     2821 aaatattcct gaattttctg tactagacca agaacgcaga aaagcggaaa tcttacaatg
     2881 tcagaatgtt tagcgaaaat tcaacgtcaa tggatggatc ttgtgaattt aggcaaaaat
     2941 tgcagcaaaa aaagtatgca aggtattatt gcacctaatc cacaagcaat aaacgtttat
     3001 agaaatacca taagaattag ccaattacgt tgtctcgaac aaacttatcc tgtttgtgta
     3061 caattagtgg gtaataccta ttttaccttt ctttttgaac aatatcggat cgcatatccg
     3121 caaagaaatt ttgatttaca tcactatggt catacctttg ccgcattttt aaaggatttt
     3181 gaattgccac acaacatact atacttaggt gatgtggcac aattggaatg ggcaatacat
     3241 catgcagaaa tgtgtgcatc ggaaccgcaa gcggttctag ataagcttgc tcgcattgat
     3301 ccggaagtat tggatacttt gcaatttcag ttaatacata gtgcaacatt actcgaatcc
     3361 gcatacccga taaaaaaaat ctgggaatta aatcaacatg caatcgtaga ggagatgatc
     3421 gctttagatc aggaaagtat gaaaataata gtttggcaca atggtcaagc tgtgcatttt
     3481 gaatctctgc aagatgcaga atgggatttt ttgcgtagct tgcaaaaaaa ctttacctta
     3541 gagcaaatat ttgatcattt acactatgtt tatcgtttgc aagaatgcga agcccaaact
     3601 atgttagcaa aatttttaca catgaattgt ttgcatatct cttattagtg caaacgcaat
     3661 tgtcctaatt actaccgaaa cgctagaatc agctctttct tgtcttttga catcattatt
     3721 acaaaacaca tattccgtgt agataatctt attttttctg aacttaatca ctttcaactc
     3781 gatcatacat tcagtgatac tttattatag gaat
//
LOCUS       DASTIB010000298         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1237581, whole genome shotgun sequence.
ACCESSION   DASTIB010000298 DASTIB010000000
VERSION     DASTIB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237581"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1685
                     /locus_tag="VFP93_05175"
     CDS             225..1685
                     /locus_tag="VFP93_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HET9844029.1"
                     /translation="MLNFGVIQNPVLDSIPLEVWGELLSFLTPGEIANLGLVCKTFHA
                     INTSESLDVVWQPFINRLKKIELSLETMPPDGVLRHAYFKKHFHRINKLQLQEIQHFQ
                     ALDQENAFAPVAQAILQKLEATMLPQGSTKLAMLEKRHELLDDLNAELIKLALHTTIL
                     DISDKGLTRFPQQLIDDPLRQEYWSALKILRCDQNQLKSLPSLDNCSSLQHLICSYNQ
                     LTSLPSLKNCQSLIELQCGNNQITSLPSLDNCRELLFLTCDHNNLKSLPNLENCLRLS
                     QLDCSNNRLTTLPNLRNCQNMTKLTCRYNQLTALPNLNHSAMLYELRCNNNLLTALPN
                     LDNCAALQWLICHDNQIVTLPSLSNCQQFKMLDCGNNKLTTLPNLDNCLGVEAIFCQN
                     NQLSVLPNLENFQAFNRLECKYNKLWTLPYSLAALFGPEWYVETLFFQKVSYLKWASE
                     KIAAYLPTFNATNANTSSAAANNMDEVDNNEPSFKM"
     gene            complement(1877..>2528)
                     /locus_tag="VFP93_05180"
     CDS             complement(1877..>2528)
                     /locus_tag="VFP93_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9844030.1"
                     /translation="EGIFEIFSQADESVSRKFGGTGLGLAISKQLVEMMGGQISLESK
                     EGVGSTFTFTIKVGVKKLKTNDVAKTIESNKEEKTQMPLHVLVVEDNELNQDVATEML
                     TNLGFEVDVADSGESALDCLTKDTYDLILLDCQMPGLDGFTTAQMIRKQESEESSSPE
                     EKKIIIAVTANALKGTKERCIEAGMDDYLTKPINFDQLKSTLKKHLNEKPNNPSLG"
BASE COUNT          800 a          515 c          398 g          815 t
ORIGIN      
        1 gttgtacgac agaccatcaa cgcgattctg acattgaagc aatagatgca tctctcctag
       61 atttaaagaa aaaaatgatc gagttaacac tataatcttt aaaaacaaaa aaaaaattga
      121 taaaatttcc ttttaacagg acaaaaggta cttgaacacg aagtattgat aagttcattc
      181 ctctataatc atgtctgatt tagtattatt gttgagacaa atctatgctt aatttcggtg
      241 ttattcaaaa cccagtatta gactccattc cgctcgaagt ttggggtgaa cttttatctt
      301 tcctaactcc tggtgaaata gcaaatttag gtttagtatg caaaacgttt catgcaatta
      361 atacgagcga atcactcgat gttgtatggc aaccctttat taatcgcttg aaaaaaatag
      421 agttaagtct tgaaaccatg ccgcccgatg gcgtattaag acacgcttat tttaaaaaac
      481 attttcatcg aattaataaa ctacaacttc aagaaattca acattttcag gcactcgatc
      541 aagaaaatgc atttgcacct gttgcccagg ccattctcca aaaacttgaa gcaacgatgt
      601 tgcctcaagg ttcaacaaaa ctagctatgc ttgaaaaaag acacgagctt ttagatgacc
      661 ttaatgccga actgataaaa ttggcactgc atacaactat tttagatatt tctgacaaag
      721 gtcttacgcg ttttcctcag caacttattg atgatccatt gcgtcaagaa tattggtctg
      781 cgttaaaaat attacgttgt gatcaaaatc aacttaagtc cttgcctagt ttggataact
      841 gctcttcatt acaacatctc atttgcagtt ataatcaact cacatcttta cccagtctta
      901 aaaattgtca atcacttata gaactacagt gcggtaataa tcaaatcacc tcgttgccga
      961 gtctagacaa ttgtcgtgag cttcttttcc taacttgcga tcataataat ctcaaatcgt
     1021 tacctaatct ggaaaattgc ttgagacttt cacaacttga ttgtagtaac aatcgactca
     1081 caactttacc aaacttgcgc aattgtcaaa acatgacaaa acttacttgt cgttacaatc
     1141 agctcacagc attacctaat cttaatcata gcgcaatgct ttatgaactg agatgtaata
     1201 ataatcttct taccgcttta ccaaatttag acaactgtgc tgcactccaa tggctaattt
     1261 gtcatgataa tcaaatcgta acattaccca gcctgagcaa ttgtcaacaa tttaagatgt
     1321 tagattgtgg taataataaa cttacaacct tacccaacct ggataattgt ttgggtgttg
     1381 aagcgatttt ttgtcaaaac aatcaactta gcgttcttcc caatttggag aactttcaag
     1441 catttaatcg gcttgaatgt aaatataata aactttggac cttaccctat agcctggcag
     1501 cactattcgg acctgaatgg tatgttgaaa ctttattttt tcaaaaagta agttatttga
     1561 agtgggctag cgaaaaaata gcggcatatc ttcctacatt taatgccact aacgcaaata
     1621 cttcgtcagc agcagcaaac aacatggacg aagtagataa taacgagcca tcattcaaaa
     1681 tgtaaatcca ctctgtaatc tattttaaag gaaggtcgcg cacagcgacc ttccttccat
     1741 attaaaagcc aagaatctca tttactctta atgtttgaat gaaggatagc ctttaacagg
     1801 acgcatgcat cctttacttt cggaaagcta agcgattttt cttaacaaca tgacattttc
     1861 tgagaccaaa aacacactaa cccaaggaag gattattggg tttttcgttt agatgttttt
     1921 taagtgtgct tttcaattga tcaaagttga taggttttgt aagataatca tccattcctg
     1981 cctcaataca acgttctttc gtgcctttta aagcattcgc tgttactgca ataattattt
     2041 tcttttcctc gggggatgaa ctttcttcgg attcttgttt gcgaatcatt tgtgcggttg
     2101 tgaatccatc taatccgggc atttggcaat cgagtaaaat taagtcatag gtatctttcg
     2161 taaggcaatc caaggctgat tcaccagaat cagccacatc tacttcaaaa ccgagattgg
     2221 ttaacatttc agtcgccaca tcttgattta attcattgtc ttcaacgact aaaacatgca
     2281 gaggcatttg tgttttttcc tctttgttac tctcaattgt tttagcaacg tcattcgttt
     2341 ttaatttctt tactcctacc tttatggtaa aagtgaaagt gctaccaacg ccttctttag
     2401 actctaaact tatttgacct cccatcatct ccaccaattg ctttgagatg gcaagaccaa
     2461 gtcccgtgcc tccaaatttt cgagaaacac tttcgtctgc ttgggaaaaa atttcaaata
     2521 taccttct
//
LOCUS       DASTIB010000299         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_886921, whole genome shotgun sequence.
ACCESSION   DASTIB010000299 DASTIB010000000
VERSION     DASTIB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886921"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFP93_05185"
     CDS             complement(<1..219)
                     /locus_tag="VFP93_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HET9844031.1"
                     /translation="MVALLAILAAIALPKYMGFTQEARTAVVEATGGSLSAGLNLAHA
                     KWEVSDTQALLDLNGDGINDTKFNQKGWP"
     gene            complement(322..1557)
                     /locus_tag="VFP93_05190"
     CDS             complement(322..1557)
                     /locus_tag="VFP93_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9844032.1"
                     /translation="MPYYFYKGRNKQGQNIEGVLEASNEHIVFVQLGRLEIIPVSIQK
                     TTKPLYVQDLSRYLPFLRPKLTELAIFAKQMYSLLKANVPVNHALQVVIESNKNAILN
                     NALTNIILDLESGFSLARCFQKQGFLFPPFISAMIDIGENTGQLEEAFHQIHHYLEAD
                     ILTRKRIKSAFRYPLIVLVAIIFALSVINFLVVPAFQRFFESLDAQLPFATRWLIEIS
                     RFSLTYWPLLVLALAVFIGVYFFIMNQPAYRYYWDKVKLQIPLVGSIIHRSILARFTR
                     SLSAAHSHGLPLLDAIQVVAKSTDNLYITDKILQMRTGLERGETLAITAKNTKVFSSL
                     VIQMIIVGEETGNIDANLMEIAEFYESDVDYDIKRLSDALEPVLVAIIAGVVLILALG
                     VFLPLWDLSSVAMHKIRGG"
     gene            complement(1566..3254)
                     /locus_tag="VFP93_05195"
     CDS             complement(1566..3254)
                     /locus_tag="VFP93_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET9844033.1"
                     /translation="MLKKIRLGDLLLKHNVLSQNEIESAVNMQSETGMKLGNVLIELG
                     FIDETNLLRFLAEQLNISFIEIKNYQIKPEILRMIPETLARRHHAVPIDKVQGSFLLA
                     MADPTDVVAFDEMSKRLPGTVEIAVVREADLLRVIDEVFRRTDEIATLAGEIEEEIGD
                     INIISEAQASEDVPIFKLLNSIFEDAIQVNASDIHIEPDKDLIRIRHRVDGVLQEQIV
                     KGKSILPALVLRLKLMAKLNISERRLPQEGRFTLKIKNKKVDIRMATMPIEGGEAIAM
                     RIFDQTGQLKKLEELGMPPNILERYRELLAMHHGMILVTGPTGCGKSTTLYASLMELN
                     KPEQKIITVEDPVEFHFSRINQVQVVPKIGLTFSRVLRSVLRHDPDILMVGEIRDEET
                     ASIALRAAMTGHLVLSTLHTNNAISSPGRLLEMGIPGYLVASALKLVIAQRLVRRVCL
                     SCQKPYRPSPQETLMLNSLMAANTEGIHFLKGKGCMHCNNTGYKGRIGIYEVLEVNLP
                     MMEALRSNNIQSFTDEARKTELFRSLIHWGLDYACMGLTTIDEVFRLLQDIDDE"
     gene            complement(3256..3870)
                     /locus_tag="VFP93_05200"
     CDS             complement(3256..3870)
                     /locus_tag="VFP93_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844034.1"
                     /translation="MKNVKEKISYYGKQIDIRSLRERFFIFLLFSILLYLLFQFLFFD
                     PLLGALNRLSANVLEKKQTLEALHIQKELQEKKHTSQAQRLNHEIAIMRKKMENKKIA
                     LVPNTLMLTHLDTFFKHESALEVVEITNKEPERIESSKFYRHPIHIILLGTYFDLLSF
                     ITHLEKFTMPVLFESIQYEVGVYPKARVELVISLITMDEDFIKF"
     gene            complement(3867..4424)
                     /locus_tag="VFP93_05205"
     CDS             complement(3867..4424)
                     /locus_tag="VFP93_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET9844035.1"
                     /translation="MKQHINLLLEDEAIFLSAKRMGFLLAFFALMLSVLVTFIIAMLF
                     LRFLELKNLEETYTKALNEVSLQPIKPDSKALQEYEGLKSKLAYVEGIQAVQSQTNKT
                     KFSAIFRALAQSKVPKTWINQIRLSNREKSIILRGNTTNPQSLIEMLKLMQSQPGLQE
                     KGITAFTLKRDKDPNAYSFKMQGHL"
     gene            complement(4421..>4985)
                     /locus_tag="VFP93_05210"
     CDS             complement(4421..>4985)
                     /locus_tag="VFP93_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844036.1"
                     /translation="IEIPSKRSIESSMIYVIVANVAHIQNILEITKNADLKVSVIDIP
                     ELALLNVFESLPSPNKTRVFISFQNDATQLIIFKNKKLLFMRRLDLVYQKSQNLEKIL
                     NDVALQVQRSLDYCSSNIMDVSSATIYLDLNEPNPDLPEKLSAILGLRVVYFDIKELL
                     DIEYSEKIPHNYTSLASVGGILRANVI"
BASE COUNT         1557 a          986 c          837 g         1605 t
ORIGIN      
        1 cggccaccct ttttgattaa atttggtatc gtttatgccg tccccattta agtccaaaag
       61 cgcttgcgta tcgctcactt cccattttgc gtgcgccaaa ttaagtccag cacttaatga
      121 accacccgtt gcttcaacaa cagcggttct ggcttcttgg gtaaaaccca tgtatttggg
      181 caaagctata gctgccaaga ttgcaagtag tgcaaccact atcaccagct caataagggt
      241 aaaccccctc aaaaaattat ttattttcat ttgcctaaac tcttactagc taatactatt
      301 atagtcttct tataaaatcc ctcagccacc tctgatctta tgcatagcta ctgaagataa
      361 atcccataat ggtaagaaca ccccaagcgc tagaataagt accacgcccg caataatggc
      421 aacaagaaca ggctctagtg catcgctcaa tcgctttata tcgtaatcaa catccgattc
      481 ataaaattct gcaatttcca ttaaattggc atcaatattt ccagtttctt ctccaactat
      541 aatcatctgt atcacaagag acgaaaaaac ctttgtgttt tttgcagtta ttgccaaggt
      601 ttctcctctt tctaaccccg tgcgcatttg taaaatttta tcggtgatat aaaggttatc
      661 tgtcgattta gcaacaactt gaatcgcgtc cagtaagggc aatccatgcg aatgggccgc
      721 agaaagcgaa cgcgtaaatc ttgccaaaat agagcgatga ataatggagc ccaccaaagg
      781 aatttgtaat ttaaccttat cccaataata gcgatacgca ggttgattca taataaaaaa
      841 ataaacacct atgaaaaccg caagcgctaa aaccaatagc ggccaatacg ttaaagaaaa
      901 tcttgaaatt tcgattaacc accgtgttgc aaaaggtagc tgtgcatcca gggattcaaa
      961 aaagcgctga aacgctggca ctaccaaaaa attgataacg cttaaagcaa aaataatcgc
     1021 tactaaaaca attaatggat accgaaatgc cgatttaata cgttttctcg ttaagatatc
     1081 ggcttcaaga taatggtgta tttgatgaaa agcttcttcg agttgtcctg tattttctcc
     1141 aatatcgatc atggcagaga taaaaggtgg aaataaaaat ccttgttttt gaaaacatcg
     1201 tgctaaagaa aaaccagact ccaaatcaag aattatattt gtcaatgcgt tatttaatat
     1261 cgcattttta ttggattcaa tcacaacctg taatgcgtga ttgaccggaa catttgcttt
     1321 cagcaaactg tacatttgtt ttgcgaaaat ggcaagttct gttagctttg gacgcaaaaa
     1381 aggcaaatag cgcgataaat cttgaacgta tagtggtttt gtagtttttt gtatagaaac
     1441 aggaataatc tctaaacgac ccaattgaac aaaaacaata tgttcattac ttgcctcaag
     1501 cactccctcg atgttttgac cttgcttgtt gcgaccttta taaaaataat atggcatttt
     1561 tacctttatt cgtcatcgat gtcttgtaat aatcgaaaaa cttcatcgat cgtagttaac
     1621 cccatacaag cataatctaa tccccaatgg attaagctac ggaaaagctc cgttttacga
     1681 gcctcatcgg taaatgattg tatattgttt gatcgtaagg cttccatcat aggaagattc
     1741 acttctaaaa cctcataaat gccaattcgg cctttgtagc cagtattgtt acaatgcatg
     1801 catcccttac cttttaaaaa atggatacct tcagtatttg cggccattaa actattgagc
     1861 ataagcgttt cttgaggtga aggtctgtag ggtttttgac aacttaaaca tactctgcgc
     1921 actaagcgtt gtgcaattac cagttttaaa gcggaagcca ccaaatagcc aggaatgccc
     1981 atctctaata ggcgccctgg acttgaaata gcattattcg tgtgcaacgt acttagaaca
     2041 aggtgtcctg tcatcgctgc acgcaatgct atactagcgg tttcttcatc gcgtatttca
     2101 ccaaccatta aaatatccgg atcgtggcgc aaaaccgatc gaagtacgcg agaaaatgta
     2161 agtcctattt taggaacaac ttgtacttga ttgattcgag aaaaatgaaa ttcgacagga
     2221 tcttctaccg ttataatctt ttgctcgggc ttgtttaact ccatgagtga ggcatagagt
     2281 gtggtagatt ttccgcagcc tgtaggtcct gtcaccaaga tcataccgtg gtgcatagct
     2341 aaaagttcgc gatatcgttc caaaatattg ggtggcatcc ctaattcttc taattttttt
     2401 aattgaccag tctgatcaaa aatacgcatc gctatagctt ctccgccctc gatcggcata
     2461 gtagccatcc gtatatctac ttttttattt ttaattttta aggtaaaacg accctcttgg
     2521 ggcaaacgtc tttctgatat attgagctta gccataagtt ttaaccgcag cactaacgcc
     2581 ggcaagatac tttttccttt cacaatttgc tcttgtaaaa ccccatccac ccgatggcgt
     2641 atacgaatga gatctttgtc cggttctata tgaatatcgg aagcgtttac ttgaatagca
     2701 tcttcaaaaa tagaattaag taatttaaaa ataggtacat cttcggacgc ttgagcctca
     2761 ctaataatat tgatatcacc aatttcttct tcaatttctc ccgcaagcgt cgcaatttca
     2821 tccgttcttc ggaatacttc gtctatcact ctaagcagat ccgcctcacg cactacagca
     2881 atttcaaccg ttccaggtaa tcgtttactc atttcatcaa atgccaccac atcggtggga
     2941 tctgccatag caagaagaaa agacccctgt actttatcaa tcggtactgc atgatgtctt
     3001 ctggctagcg tttctggaat catccttagt atttcaggtt taatttgata atttttaatt
     3061 tcgataaacg aaatatttaa ttgctcagcc aagaatcgca ataaattggt ttcatcgata
     3121 aaacctaatt caattagaac atttcccaac ttcatgccag tttcactttg catattcacg
     3181 gcagactcta tttcgttttg tgataaaacg ttatgtttta ataacagatc acctaaacga
     3241 atttttttta acatactaaa atttaataaa atcctcatcc attgttataa gactgataac
     3301 taattccacc cgggcttttg gatatacccc tacttcatat tgaatggatt caaacagcac
     3361 tggcatggtg aatttttcca aatgggtaat gaaactgagc aaatcaaaat aagtaccgag
     3421 taaaattatg tgtatcgggt gtcgataaaa tttactactc tcaatgcgtt ctggttcttt
     3481 attggttatt tcaaccactt ccaaagcact ttcatgttta aaaaaggtgt ctaaatgtgt
     3541 gagcatcaag gtattgggaa ctagcgcaat ttttttattt tccatttttt tgcgcataat
     3601 agcgatttca tgatttaatc tttgcgcttg acttgtgtgt tttttttctt gcaattcttt
     3661 ttgaatatgt aacgcttcta aggtttgttt tttttccaac acattagcac tcaagcgatt
     3721 taatgcacct aaaagcggat caaaaaataa aaattgaaaa agcaaataca ataaaatact
     3781 aaataaaaga aaaataaaaa agcgttctct tagagatcgt atatctattt gcttaccata
     3841 atagcttatt ttttctttta catttttcat aaatgacctt gcattttaaa agaataagca
     3901 ttgggatctt tatctcgttt taaggtgaat gctgtaattc ctttttcctg taacccgggt
     3961 tgagattgca tcaacttaag catttcaata agcgattgag gatttgtggt atttcctcgt
     4021 aatataatac ttttttcgcg gttgctgagt cgtatttgat taatccaggt tttaggtacc
     4081 tttgattgcg ccagcgctct aaaaatagca gaaaatttag ttttattcgt ttgggattgt
     4141 actgcttgga taccttcaac atacgcaagc ttacttttta agccttcata ttcttgcaaa
     4201 gccttgctat caggcttgat gggttgcaga gaaacttcgt taagcgcctt ggtatatgtt
     4261 tcctctaaat ttttcaattc taaaaagcgc aagaataaca tcgcgataat gaaagtaaca
     4321 agaacgctta acataagcgc aaaaaatgct aataaaaatc ccatgcgttt tgcacttaaa
     4381 aaaattgctt cgtcttctaa caataaatta atatgttgtt tcatataacg tttgctcgca
     4441 atattccgcc aacagaagct aaacttgtgt aattgtgggg aattttctca ctgtactcga
     4501 tatctaataa ttcttttatg tcaaaatata ctacgcgcaa gcccaaaata gcagataatt
     4561 tttcaggcaa gtctggattc ggctcgttta aatcaaggta aatagtggca cttgaaacat
     4621 ccataatatt agaggaacaa taatcgagag aacgttgaac ttggagtgct acgtcattaa
     4681 gtattttttc caaattttga gatttttggt aaaccaaatc caatcgccgc ataaaaagta
     4741 attttttgtt tttaaaaata ataagttgtg tagcatcgtt ttgaaagctg ataaaaacac
     4801 gtgttttatt tggagaaggc agagactcaa atacatttaa taatgctaat tcgggaatat
     4861 ctataacgct gacctttaaa tctgcatttt ttgttatctc taaaatattt tgaatatgcg
     4921 caacattggc aacgatgaca taaatcatgc ttgattcaat acttcttttt gatggaatct
     4981 caata
//
LOCUS       DASTIB010000300         6235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1394344, whole genome shotgun sequence.
ACCESSION   DASTIB010000300 DASTIB010000000
VERSION     DASTIB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394344"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFP93_05215"
     CDS             complement(<1..473)
                     /locus_tag="VFP93_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844037.1"
                     /translation="MIGIINALKHSKPFLHKIYKAKNGNLEQRSSDVEHFDVTGQIPR
                     SIVFDKTGNIYSILERIGQGAFGVVKLAFNVTTAQLAAVKIQKISSDQRSAQDQLQIE
                     EDLTKNLGRFYGSLYRTNPKGVKKHYLFMEYLRSDQNIFTKIKEASFEDKIKIAL"
     gene            664..1275
                     /locus_tag="VFP93_05220"
     CDS             664..1275
                     /locus_tag="VFP93_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844038.1"
                     /translation="MFSKACILLTEKGTELKIFMGITNSRYYEIKNCVYLEAYQNFIM
                     VKNHYRLFEKILQSNNTPRENLEILREFQDKIRLEKGSPSNSLKQNLLFLHENTKLHP
                     DHAREMVMLHGISGWHGSPYDVHDERLCDMIKFLEEYCFAKYESAARRNYINGFKYQQ
                     IPWTDPKWILDYCQPSRSSDFDNGRKQAKITSKSAKGHNIKFV"
     gene            1360..2265
                     /gene="glyQ"
                     /locus_tag="VFP93_05225"
     CDS             1360..2265
                     /gene="glyQ"
                     /locus_tag="VFP93_05225"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET9844039.1"
                     /translation="MKTIAKPPQTFDELVQAFHKFWADQGCVILQPLDMEVGAGTFHP
                     ATFLKAIGPEPWYAAHVQPSRRPTDGRYGDNPNRTQMFHQYQVVLKPHPLEIQELCID
                     SLRAIGIDPNQNDIRFVEDNWESPTLGAWGLGWEVWLNGMETIQFTYFQQVGGLECKP
                     VMGELAYGLERLAMHTQNKESMFDVVWAQTPHGMVTYGDIFHQNEVEMSAYNFEYADI
                     AILLQHFDDYEGVANKLIAAQKPLPAYELVLKASHTFNLLDARNAIGVTERQRYILRV
                     RTLAKAVAETYYKMRESLGFPLLKA"
     gene            2270..4366
                     /gene="glyS"
                     /locus_tag="VFP93_05230"
     CDS             2270..4366
                     /gene="glyS"
                     /locus_tag="VFP93_05230"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET9844040.1"
                     /translation="MKQHEDLLIEIGAEELPPEALDTLAANWAKHFSMELEKQNIVTT
                     IEHFSTPRRLALLAKNIPYQQTDRIIQKRGPALSAAFDAQNNPTKATLGFAKSNNVQV
                     EQLEKLETEKGSWLIYNAQEKGQAFAQLLPQLLHNSLQATFKGKKTMRWADKDVDFLR
                     PVHWCVILHGTQVITLPVLGVESGQETYGHRVHHPKNIRLNHPQDYVNLLRESYVLAS
                     YAERKAAIEEQINKIATSLHATPVVEPKLLDLVTNLTEWPKALVANFDPAFLEVPAEV
                     LITAMQHHQKSFALRDHNGNLLSQFILIANIDAKVTDKVIRGNERVMQARLNDARFFY
                     VQDKKIPLIDRLTELKHVIFQHKLGTLFDKTQRVKNLMIEIYSRIAQHTSKSSVDKGI
                     SSAERAALLSRCDLLSKMVLEFPELQGIMGSYYAKQDNEETEVAQAIQEMYYPRFSGD
                     KLPESEVGLALSISDKLDTLVGIFSIGQAPTGDKDPFALRRQAVSIVRMLLEKSLALN
                     LQELISLAASNYASNISKEVQAAILNFCLERARSLFVEQGIQPKIFEAVHAICDFSVP
                     LDFQQRLHAVANFTKLPESESLAASDKRVRNILLKSNFSTNENGVLTEFLIENAEKAL
                     FEEIENLKITTQPLLQQRKYQEALSKLAKIRTCVDAFFDNVMVNVEDPKLRQNRIHLL
                     ANLNQLFSSVADISKL"
     gene            4363..4914
                     /gene="gmhB"
                     /locus_tag="VFP93_05235"
     CDS             4363..4914
                     /gene="gmhB"
                     /locus_tag="VFP93_05235"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HET9844041.1"
                     /translation="MKNTWVLLDRDGVINFDSEDYIKSPEEWIPIPNSLEAIRLLNQK
                     GYSVAIATNQSGIQRGLYSEQILEQIHQKMTDSLSKLGGAIAAIHYCPHGPNDGCMCR
                     KPKPGLLMQIAHKFSLDLTKTPYVGDSWRDCQAAISAGCKPVLVKTGNGLITIKKYPN
                     EIEHIPVFSDLYNFAYSLPFVKE"
     gene            5184..5738
                     /locus_tag="VFP93_05240"
     CDS             5184..5738
                     /locus_tag="VFP93_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9844042.1"
                     /translation="MAKHICGIRYEIEGLENLPATNAVVLCNHQSTWETLFLQTLLPM
                     QTWVLKKELLWIPFFGWGLGLLQPIAIDRNKKSSIKSLMETGRQRLTEGRYIIVFPEG
                     TRLKPGEQRKYSRSGAALAQATSSYILPIAHNAGNFWPKNSFLKYPGVIKVCIGKVLD
                     PKHFTVDSLTQSAQEWIYSQVAEA"
     gene            complement(5735..>6235)
                     /gene="gyrB"
                     /locus_tag="VFP93_05245"
     CDS             complement(5735..>6235)
                     /gene="gyrB"
                     /locus_tag="VFP93_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065835.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HET9844043.1"
                     /translation="KKSLKGFSVKFDIKEDEIEGLYAPIARLLKHGIAYEYPFLPDFF
                     SSKEYQVLTNLGTKINNLLQVGSFVKRSEKQKPVESFQDALSWLMEEAKRGQQIQRYK
                     GLGEMNAEQLWETTMDPSSRRLMKVTIEDVISADQIFATLMGDQVEPRRDFIEHNALY
                     VENLDV"
BASE COUNT         1958 a         1187 c         1194 g         1896 t
ORIGIN      
        1 cttaacgcaa tttttatttt atcttcaaaa gacgcttctt ttatctttgt aaaaatattt
       61 tggtccgatc gcaaatattc cataaataaa taatgctttt tgacaccttt tgggttggtg
      121 cgataaaggg atccataaaa acgacctaga ttcttggtaa gatcttcttc aatctgtagc
      181 tgatcttgtg cagacctctg atcagaagaa attttttgaa tttttacagc agccaattgg
      241 gcagttgtta cattaaaagc aagcttcact acaccaaagg ccccctggcc tattctttct
      301 aaaatcgagt agatattgcc tgttttatca aaaacgatgg aacgtggtat ttgtcctgta
      361 acatcaaaat gctcaacgtc agaactgcgt tgttcgaggt tgccattctt tgccttatat
      421 attttatgta aaaaaggttt actgtgtttt agtgcgttta ttattccaat catgaacatt
      481 accttagatt ttttttgaac aatgatatac tattttagat aattgcgaac cgttttgtgc
      541 aagtaagata tactttatca aagtatttaa aggatgcctt atggttacta ttggtgattt
      601 gtcatcacgc catggagtta tttcgttttt agcctagaat aaaactctac ttaggaaaaa
      661 attatgtttt caaaagcatg tattttatta acagaaaagg gaactgaact caaaatcttt
      721 atgggaataa ctaatagtcg ttactacgaa ataaaaaatt gtgtttactt ggaggcctat
      781 caaaatttta ttatggtgaa aaatcattat cgattattcg aaaaaatttt acaaagcaac
      841 aacactccca gagagaatct tgagatttta cgagaatttc aagataaaat acgcctggag
      901 aaaggttcac cttccaattc tttaaaacaa aatttattat ttttacatga aaacacaaag
      961 ttacatcccg accatgctcg tgaaatggtt atgcttcatg gtatttccgg ctggcacggt
     1021 tcaccttacg atgtacatga tgaacgcttg tgcgacatga taaaatttct cgaagagtat
     1081 tgttttgcta agtatgaaag tgcggcaagg cgaaactata ttaacggttt taaataccaa
     1141 caaataccct ggaccgatcc aaaatggatc ttagactatt gccaaccatc gagaagttcg
     1201 gactttgaca atggccgcaa acaagcaaag atcacgagca agtcagccaa ggggcataat
     1261 ataaaatttg tttaagtacc cggcatgaaa actaatttaa gtaccaacca attccttgta
     1321 tactatttag ttttctacgc cataaaatca gatagctcca tgaaaacaat agcaaaaccg
     1381 ccgcaaacat ttgatgaact ggtgcaggca tttcataaat tttgggctga tcaaggttgt
     1441 gtgatcctac aacctttaga catggaagtg ggtgcaggta catttcatcc agcgacattt
     1501 ttaaaagcta ttggaccaga accttggtat gcagctcatg tgcaaccctc acgtcgccca
     1561 actgatggta gatatggcga taacccaaac cgcacccaaa tgtttcatca ataccaagtg
     1621 gtattaaagc cgcatccctt agaaatccaa gaattgtgta tagattcctt gcgcgccatt
     1681 ggaattgatc ccaaccaaaa cgatatccgt tttgtagaag ataattggga gtcaccaaca
     1741 cttggagctt ggggtctagg gtgggaagtt tggctcaatg gaatggaaac tattcaattt
     1801 acctattttc aacaagtggg tggtttagaa tgtaagccgg tgatgggtga gttagcctat
     1861 ggcttggagc gcttagccat gcacacccag aacaaagaaa gcatgtttga tgtggtttgg
     1921 gcgcaaacac cccatgggat ggtaacgtat ggcgatattt ttcatcaaaa tgaagtagaa
     1981 atgtccgcgt acaattttga gtatgctgat atagctattt tattacagca ttttgatgat
     2041 tatgaaggag tagccaataa actaattgca gcgcaaaaac ctctgcctgc ctatgaatta
     2101 gtgttaaaag catctcacac gtttaattta ctcgatgcac gtaatgctat tggtgtgaca
     2161 gaacgccaac gctatatctt acgcgttcgc acccttgcta aagcggtggc agaaacttac
     2221 tacaaaatgc gcgaatctct tggttttcca ttgttaaaag cctaaaacta tgaagcaaca
     2281 cgaagattta ttaattgaaa ttggtgcgga agaattacca cccgaagcgc tcgacacttt
     2341 agctgcaaat tgggccaaac atttcagtat ggagcttgaa aaacaaaata tagttacaac
     2401 tattgagcac ttctctacac cgcgtagatt agcattatta gccaagaata taccgtatca
     2461 acaaaccgat agaattatcc aaaaacgggg gcccgcatta agtgccgcat ttgatgcaca
     2521 gaataatcct accaaggcga ctttgggttt tgcaaaaagt aataatgtac aagtagaaca
     2581 gctcgaaaaa cttgaaaccg aaaaaggttc ttggttaatc tacaatgcgc aggaaaaagg
     2641 ccaagctttt gcacagttgt taccgcaatt attacacaat tccttgcagg caacattcaa
     2701 aggcaaaaaa accatgcgat gggcagataa agacgtagat tttttgcgtc ctgtgcattg
     2761 gtgtgtgatt ttgcatggca cgcaggtaat tacgttgcct gttttagggg ttgaatcggg
     2821 tcaagaaacc tatggtcata gagtgcatca tccgaaaaat attcgtttaa accatcctca
     2881 agattatgta aatttattgc gagaaagcta tgtattagct tcttatgccg aacgcaaagc
     2941 agcgattgaa gagcaaatta ataaaatcgc cacctctttg catgcaacac ccgtagtaga
     3001 gcctaaatta ttggatcttg taaccaattt aaccgaatgg ccaaaagctc ttgttgctaa
     3061 ctttgatcca gcatttttag aagtccccgc agaggtgctc atcacagcca tgcagcatca
     3121 ccaaaagagt tttgccttaa gagaccacaa tggcaactta ttgtctcaat ttattttgat
     3181 cgcaaatatc gatgccaaag ttacggataa agtgatccgc ggtaatgagc gcgtaatgca
     3241 agcacgatta aatgatgccc gattttttta tgttcaagat aaaaaaatac ctttaatcga
     3301 tcgtctaacc gaattaaagc atgtcatttt tcagcataaa ttaggcactt tgtttgataa
     3361 aacacaacgt gttaaaaatt taatgattga aatttacagc cgcatcgctc agcacacctc
     3421 gaaatcttcc gtggataaag gaatctcgag cgcagaacgt gcagcgcttt tatcaagatg
     3481 cgacctactt agtaaaatgg tattggaatt cccggaactc caaggaatca tgggctcgta
     3541 ttatgcaaaa caagacaatg aggaaacaga agtcgcccaa gctatccaag aaatgtacta
     3601 tccaaggttt tcaggcgaca aattgccaga atctgaagtt ggactagcgc taagcatttc
     3661 agacaaacta gacacattag tgggtatttt tagcattgga caagcgccaa ccggcgataa
     3721 agatcccttc gcgctacgac gccaagcagt cagcatagtg cgcatgcttt tagaaaaatc
     3781 gttggctctt aatttgcaag aactaatatc tctcgcggca tccaattatg caagtaacat
     3841 atccaaagaa gttcaagctg ccattttaaa tttttgctta gagcgcgcca ggtctttatt
     3901 tgtagaacaa gggattcaac ccaaaatttt tgaagccgtg catgccattt gtgatttttc
     3961 ggttccattg gattttcaac aacgtttaca cgctgtagca aatttcacca aactgcctga
     4021 gtcagaaagt ttagctgcca gtgacaaacg tgttcgcaac attttattga aaagcaattt
     4081 ttctaccaac gagaatggtg tcctaacaga atttttgata gaaaatgcag aaaaagctct
     4141 ctttgaagaa attgaaaatt taaaaataac aacacagcca ttgctgcaac aaagaaaata
     4201 ccaagaggca ctttcaaagc tggctaaaat tcgcacctgt gttgatgcgt ttttcgataa
     4261 tgttatggta aatgttgagg atccgaaact gcgacaaaat cgtatccatt tacttgcgaa
     4321 tttaaatcaa ctgtttagca gtgtagctga tatatcaaaa ctatgaagaa tacttgggta
     4381 ttattagatc gagatggtgt tataaacttc gattctgagg attatataaa atcacccgaa
     4441 gaatggatcc ctatacctaa tagtttagaa gccattcgct tgttgaatca aaaaggctat
     4501 tcggttgcaa tagctacgaa ccagtctggc attcaaagag ggctttatag cgaacaaatt
     4561 ttggagcaaa ttcaccaaaa gatgaccgat tctctatcga aactaggggg agctattgct
     4621 gcaattcatt attgtcctca tggcccaaac gatggatgta tgtgtcgcaa accaaaacca
     4681 ggtctgttga tgcaaattgc gcacaaattt tccttagatt taaccaaaac accctatgtt
     4741 ggcgattcct ggcgcgattg tcaggcagcg atttctgctg gctgtaagcc cgttttagta
     4801 aaaactggaa atggattaat tacaataaaa aaatacccta acgaaataga acatattccg
     4861 gttttttccg atttatataa ctttgcttac tcattaccct ttgttaaaga ataatttgtg
     4921 ctattagaaa atgactcttg agttggtatt ctgtagtcat tgagttagaa tatcctctaa
     4981 atttcaaacg cttcttatta gggttttgaa tgaagaaaaa aaggcagtcg tattcttgga
     5041 tgatcttctt tcgttccttc ctttttaata tcttgcttgg tttgcttacg attttctttt
     5101 cgacaaccgg ttttcttttt ttattagccc cttttaaagt gcgttatttt tatataactc
     5161 gctggtctca tttggttatt tttttggcca aacatatttg tgggatccga tatgaaattg
     5221 aaggattaga aaatttaccc gcaaccaatg ctgtcgtatt gtgtaaccat caatccacct
     5281 gggaaacatt atttttacaa accttactac ccatgcaaac ttgggtttta aaaaaggaat
     5341 tattgtggat cccgtttttt ggttggggct tagggttatt acaacccatc gccatcgata
     5401 gaaacaaaaa gagctcaatt aaatctttaa tggaaacggg tagacaaaga ctaaccgaag
     5461 gtcgttatat tattgttttt ccagaaggca ctcggcttaa gcctggagaa caaagaaaat
     5521 attctcgaag tggtgcagct ttagcgcagg ctacttcttc ttatatttta cctattgcgc
     5581 ataatgctgg taacttttgg ccgaaaaata gttttttaaa gtatccaggc gtgattaagg
     5641 tgtgtattgg gaaagttcta gatcctaagc actttacggt cgatagtctt acgcaaagtg
     5701 ctcaagaatg gatttattct caagtagcgg aagcttaaac gtctaaattt tcgacgtata
     5761 aagcattgtg ttcaataaaa tcacgtcttg gctcaacttg atctcccatc aatgtggcga
     5821 atatttgatc ggcagagatc acatcttcaa tggttacttt catgagacga cgagagctag
     5881 gatccatggt tgtctcccaa agttgctcag cattcatctc gcctaaccct ttatagcgtt
     5941 ggatctgttg accacgtttg gcttcttcca tgagccagga taatgcatct tggaaacttt
     6001 ccaccggttt ttgtttttca cttcgtttta caaaagagcc tacttgtaac aaattattaa
     6061 tctttgtgcc taaatttgtt aaaacttgat attcttttga actgaagaag tctggtaaaa
     6121 aagggtactc atacgctata ccatgtttta gtaggcgcgc tataggggca taaagtcctt
     6181 ctatttcatc ttcctttata tcaaatttta cgctaaatcc tttcaacgat ttctt
//
LOCUS       DASTIB010000301         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_649090, whole genome shotgun sequence.
ACCESSION   DASTIB010000301 DASTIB010000000
VERSION     DASTIB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649090"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="rapZ"
                     /locus_tag="VFP93_05250"
     CDS             <1..723
                     /gene="rapZ"
                     /locus_tag="VFP93_05250"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET9844044.1"
                     /translation="SFAHPRLAVSIDARNLPKDLNELKCVISELKKPEMHCEILYLDS
                     DENVILARFSETRRRHPLTRDGLSLQEAIRQEKFLLSPLSHMADVTIDTTNLTKHQLC
                     SLIFQKLANEPIDHLQVLLQSFGFKNGIPSDADFIFDVRCLPNPYWAPNLRLLTGLDK
                     EVSHFLQSQPEVNLMIEDILVFLNNWIPKFKANNRSYLTVGIGCTGGQHRSVYIAEKL
                     SHSIHGQHKQVHIRHRDLLQVK"
     gene            723..1205
                     /locus_tag="VFP93_05255"
     CDS             723..1205
                     /locus_tag="VFP93_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET9844045.1"
                     /translation="MVGILLITHSGIGRALTKTLALTFGTIPLRIEELAIESDPDPEK
                     LVQEAAKIAQRLDQGSGVLILTDMYGSTPSNIALKLQTLKLQTRVIAGLNLPMLFKIL
                     NYSHLSLPLLAAKAMHGGREGIVETKSHRGLIYVNIDRLKKHKYRLLKTKTFKRLKHV
                     "
     gene            1202..1471
                     /locus_tag="VFP93_05260"
     CDS             1202..1471
                     /locus_tag="VFP93_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9844046.1"
                     /translation="MIRKRVQIINKLGLHARAAAKFVQLASSFYSEIEISHAGKTVNG
                     KSIMGIMMLAASQNTWIELTISGSDAEKAMITLEELIQNRFGESS"
     gene            complement(1545..>2107)
                     /locus_tag="VFP93_05265"
     CDS             complement(1545..>2107)
                     /locus_tag="VFP93_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844047.1"
                     /translation="WKFHALSVFMTEKSIYHKPHFGLILNNHVELSERIVQNLSRVES
                     LKLLRLIPACFQKLKQQLCFVVQSKFGTNSFEGYRALASILQEVSFNDTGSIDKAFLN
                     QWPQWLKKGIKENKNLEELLILEESKEKSALISESKNFCKNLEQDWIIDVKESDPIVS
                     PHSSINRLNEEFLKIDLTHSSKHKFP"
BASE COUNT          667 a          386 c          379 g          675 t
ORIGIN      
        1 tcatttgccc accctcgtct cgctgttagc attgatgcca gaaatctacc taaagactta
       61 aatgagctta aatgtgtaat ttccgaacta aaaaaacctg aaatgcactg cgaaatatta
      121 tatctcgatt cggatgaaaa cgttattctt gcccgtttta gtgaaaccag acgacgacat
      181 ccgttaacac gtgatggttt atccctacag gaagcaatcc gacaagaaaa atttctgtta
      241 agcccactgt cgcatatggc agatgttact attgatacaa ccaatttaac taagcatcaa
      301 ttatgcagtt taatatttca aaaacttgcg aacgaaccta ttgaccatct gcaggtattg
      361 ttacaatctt ttggttttaa gaatggtata cctagtgatg cagattttat ctttgatgtt
      421 cggtgtttgc caaaccctta ttgggcacct aatcttcgtc tactcacagg actggataag
      481 gaggtgagtc attttttgca atctcaaccc gaagttaact taatgattga agatatttta
      541 gtttttttaa ataattggat ccccaagttt aaagcgaata atcgcagcta cctcaccgta
      601 gggattgggt gtactggggg acaacatcgc tcggtttata tagcggaaaa attgtcgcac
      661 tccattcatg gacaacataa acaagttcat atacggcatc gagatttgtt acaagtaaaa
      721 taatggtcgg aatactttta attacacata gtggaattgg tcgcgcttta actaaaacgc
      781 ttgctctaac ctttgggact attcccttgc gtattgaaga gcttgctata gagtcagatc
      841 cagatccgga aaaattggtg caggaggcgg caaagatagc acagcgctta gatcaaggta
      901 gtggggtttt gattttaacc gatatgtatg gttcaacacc ttccaatata gcgctcaaac
      961 ttcagacttt aaaattacaa acacgtgtta tcgctggttt gaatttgcct atgctgttta
     1021 aaattttaaa ttattcgcat ctttctctac cacttttggc tgcaaaagcg atgcatggtg
     1081 gacgtgaagg tatcgttgag actaaatcgc atcgaggatt aatttatgta aatatcgatc
     1141 gattaaaaaa acataaatat cgtttattaa aaaccaaaac gtttaaacgt cttaaacatg
     1201 tatgatacgt aagcgtgtac aaatcattaa caaattaggt cttcatgcac gcgctgctgc
     1261 aaaatttgtt caattggctt caagctttta cagtgaaatt gaaatctcac atgcaggaaa
     1321 aactgtgaat ggcaaaagta tcatgggaat tatgatgctt gctgcaagcc aaaacacctg
     1381 gattgaattg accatatctg gctctgacgc agagaaagct atgattacat tagaagaatt
     1441 aattcaaaat cgatttggcg aatcttccta atgacaatta atacatctag acttgcgaag
     1501 gcaagattta tatttaattg cgaaaattgg aacgcagcca tggattaagg aaatttatgc
     1561 ttcgagctat gagttaaatc tatctttaaa aattcctcat ttaacctgtt tatagaacta
     1621 tggggtgaga cgataggatc agattctttt acgtcaatga tccaatcttg ctccaagttc
     1681 ttacaaaaat ttttgctttc acttattagt gctgattttt cttttgattc ttctaaaatt
     1741 aataattctt ctaaattttt gttttctttt atgcctttct tcaaccattg tggccattgg
     1801 ttcagaaatg ctttatctat actgcccgta tcgttaaaag acacctcttg cagaatcgag
     1861 gctaaagcac gatacccttc aaaactgttc gttccaaatt tactttgtac cacaaaacat
     1921 agttgctgtt ttaatttttg aaagcaagca ggaattaatc gcaatagttt taaactttct
     1981 acacgcgata aattttggac aatccgctct gataattcca catgattatt aagaataagg
     2041 ccaaaatgtg gcttatggta aatagatttt tccgtcatga aaacactaag agcatggaat
     2101 ttccaat
//
LOCUS       DASTIB010000302         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_23144, whole genome shotgun sequence.
ACCESSION   DASTIB010000302 DASTIB010000000
VERSION     DASTIB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23144"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFP93_05270"
     CDS             complement(<1..543)
                     /locus_tag="VFP93_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET9844048.1"
                     /translation="MKQAGRSKIQKNMQIGRRLREGALILLISLAIYILIALVSYHVN
                     DPGWSYSGQTQQVSNLAGKAGAWLSDILYSLFGYIAYLFPLAVVFIGIAIYQGQEFLN
                     KHQNSYSKSFWLLRIVGLTTALISGCGLTTLHFYYPINPLPMSSGGLLGAAVKMGLVS
                     QLSYLGSTVILLAFFLTGITL"
     gene            747..1709
                     /gene="trxB"
                     /locus_tag="VFP93_05275"
     CDS             747..1709
                     /gene="trxB"
                     /locus_tag="VFP93_05275"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET9844049.1"
                     /translation="MAEVKHCKLIILGSGPAGYSAAIYAARANLKPTLIAGLQKGGQL
                     TTTTDVDNWPGDVEGLQGPDLMQRMEAHAKKFATEIIEDHIMKVDLSQRPFLLEGDSK
                     NVYTAQALIIATGASAKYLGLPSEEKFLGKGVSACATCDGFFFRKQRVAVIGGGNTAV
                     EEALYLSNLAAQVTLIHRRDKLRAEKILIDKLMHKVQNGNVDILWNYTVDEIVGNEKG
                     VQGIRLQHVHSSTMHTMDLEGVFIAIGHTPNTKIFEGQLVMDHGYIQINSGLKGNATA
                     TSIPGVFAAGDVCDQIYRQAISSAGFGCMAALDAEKYLDDLDLE"
     gene            1717..2406
                     /gene="aat"
                     /locus_tag="VFP93_05280"
     CDS             1717..2406
                     /gene="aat"
                     /locus_tag="VFP93_05280"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET9844050.1"
                     /translation="MKIPFLHNNDYSFPDPNLALKEPNGLLAVGGDLSMGRLLNAYRQ
                     GIFPWYEESDPIVWWSPDPRAILFFEDFIITKRFSRTLHSESYTIFLDRNFDAVITAC
                     QNPRPKQESTTWITPEMKKAYCALFEAGFCHCIEVLQENTLIGGIYGVSLGHAFFGES
                     MFSFKSNGSKIALFYLIEKLKTLGFSWLDCQIGSPHLERLGAKLIPRSDFHRLLQAAN
                     VFPTILGPWTY"
     gene            2553..2813
                     /gene="infA"
                     /locus_tag="VFP93_05285"
     CDS             2553..2813
                     /gene="infA"
                     /locus_tag="VFP93_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9844051.1"
                     /translation="MAKEDHIEMEGTVVENLPNTTFRVELDNKHVVTAHISGRMRKNY
                     IRILTGDRVTVELTPYDLTKGRIIFRNKDKDGKMGRGERDRE"
     gene            complement(2814..>3112)
                     /gene="clpA"
                     /locus_tag="VFP93_05290"
     CDS             complement(2814..>3112)
                     /gene="clpA"
                     /locus_tag="VFP93_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577549.1"
                     /note="ATPase and specificity subunit of the ClpA-ClpP ATP
                     dependent serine protease; directs protease to specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HET9844052.1"
                     /translation="ETTICKVVDKFIHELEEKLLDKDVTLHVDEAARTWFVKNGYDHK
                     MGARPMARLIQEELKKPLADELLFGSLHAGGHIDVTVKNNKLNLNICEKVQDKT"
BASE COUNT          993 a          577 c          603 g          939 t
ORIGIN      
        1 taaagtaata cccgttaaaa aaaatgccaa taatattacc gttgatccta aatagcttaa
       61 ttgagaaaca agccccattt ttaccgcagc accgagcaag ccacctgaac tcatcggcaa
      121 ggggttaata ggataataaa aatgtaaagt agttaaacca caacccgata tcaatgcagt
      181 cgttaaacca acaattcgta acaaccaaaa agatttagaa tacgaatttt ggtgtttatt
      241 taaaaattcc tgaccttggt aaatggcaat tccgataaat acaactgcca atggaaacaa
      301 atacgcaata tagccaaaaa gcgagtataa gatatcagaa agccaagccc ctgctttgcc
      361 tgccaagtta gatacttgtt gggtttgacc actataagac caacccggat catttacatg
      421 ataactaact aaggcaatta aaatataaat tgccaaggaa attaacagga ttaaggcacc
      481 ttctcgaagt cggcgcccta tttgcatatt cttttgaatt ttacttcgtc ctgcctgttt
      541 cattctaact cttatatttt gattttattg ccaagttatt ttaccactaa gttatactag
      601 gccacagcgc gtcatacttc cgagtattta ataatgaata ctttaagaga agtacttcgg
      661 ctaacaatat ataatacttt gatgaccttt gacatcgctt tcattatcgt acaaaaaaac
      721 aaaccttcac tatatatagg tgttctatgg cagaagtaaa gcactgcaaa ttaattattt
      781 taggctcagg accggcgggt tatagcgcag ctatttatgc tgctagagca aatttaaaac
      841 ccacgctaat agctggactc caaaaaggcg gacaacttac aactaccaca gatgttgata
      901 actggccagg cgatgtcgaa ggtttacaag gtccggatct tatgcaacgt atggaagcgc
      961 atgcaaaaaa atttgccaca gaaattattg aagatcacat aatgaaagtc gatttatccc
     1021 aacgtccttt tttattagaa ggtgattcaa aaaatgtgta tacagcacaa gcccttatta
     1081 tcgccacggg tgcttcggct aaatatttag gcttaccatc agaggaaaaa tttctcggca
     1141 aaggtgtttc cgcttgtgca acctgtgatg gattcttctt tcgaaaacaa cgagttgccg
     1201 tgattggagg tggtaatacc gccgtggaag aagcactgta tttatccaat cttgcggccc
     1261 aagtaacttt gatacatcgg agagataaat tgcgcgcaga aaaaatattg atcgataaac
     1321 tgatgcataa agtacaaaat ggaaatgtgg atattttgtg gaattataca gtcgatgaaa
     1381 tagtaggcaa tgaaaaaggt gttcaaggca ttcgtttgca acatgtgcat tcctcaacta
     1441 tgcacactat ggatttggaa ggcgtattta tcgcaatcgg tcacacacct aatacaaaaa
     1501 tttttgaagg tcagctcgtg atggaccatg gctacattca aataaattct ggtttaaaag
     1561 gaaatgccac tgcaacaagt attccgggtg tttttgccgc gggagatgta tgtgatcaaa
     1621 tttatcgtca agctatttct tcagcaggat ttggttgtat ggcagcttta gatgccgaaa
     1681 aatatttgga tgatttagat ctggaataga atacatttga aaattccttt tttacataac
     1741 aacgactata gcttcccgga tcctaaccta gcgttaaagg aacccaatgg gttgctagcg
     1801 gttggtggag atttgtcgat gggcagatta ttaaacgctt atcgacaagg aatttttccg
     1861 tggtacgagg aatcggatcc cattgtatgg tggtcaccgg atccgcgcgc tatattattc
     1921 tttgaagatt ttatcataac aaaaagattt tctcgtactt tacatagcga atcatacact
     1981 atatttctcg atagaaattt tgatgcagta attaccgcgt gtcaaaatcc aaggccaaaa
     2041 caagaatcga caacctggat tacaccggaa atgaaaaaag cttattgcgc tttatttgaa
     2101 gctggatttt gccattgtat agaagtcttg caggaaaata ctttaatcgg tggtatctat
     2161 ggcgtaagtt taggacatgc tttttttggc gaatctatgt tttcgtttaa aagtaatggt
     2221 tcaaaaatcg ctttatttta cttaatagaa aaacttaaga ccctaggctt ttcatggctg
     2281 gattgtcaaa ttgggtctcc acatctggaa aggctaggtg ccaaacttat tcctaggtcc
     2341 gactttcata ggttattgca agcagccaac gttttcccaa cgattcttgg accatggaca
     2401 tactaacctt ctgaatttaa tatatatatt taattaatta ttttgcaatt ggtgtgtcct
     2461 agcatatttt tattgtggat aatcctaacc agaatggttt ttagaatagg tttttggtag
     2521 catagcgaac gtaaaaacta taaaggtaat gtatggcgaa agaagatcat attgagatgg
     2581 aaggaactgt agttgaaaac ctccccaaca ccacatttcg cgtcgaattg gataataagc
     2641 atgtagttac agcgcatatt tccggtagaa tgcgtaaaaa ttatattcga attttaacgg
     2701 gagatagagt aacggttgaa ctcaccccct acgatttaac gaaaggtaga attattttta
     2761 gaaacaaaga caaagatggg aaaatgggtc gtggtgagcg ggatagagaa taattacgtt
     2821 ttgtcctgca ctttttcaca aatattgaga ttaagtttat tattttttac ggtgacatcg
     2881 atgtgccctc ccgcgtgtaa gcttccgaac aataactcat cggcgagagg tttttttaat
     2941 tcttcttgta tcaaccgtgc catgggccgc gcacccattt tatgatcata cccattcttt
     3001 acaaaccacg ttcgcgctgc ttcatcaaca tgtaatgtaa catctttatc gagcagcttc
     3061 tcttccaatt catggatgaa tttgtccact actttacaaa tagttgtttc at
//
LOCUS       DASTIB010000303         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_905845, whole genome shotgun sequence.
ACCESSION   DASTIB010000303 DASTIB010000000
VERSION     DASTIB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905845"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..541
                     /gene="ampD"
                     /locus_tag="VFP93_05295"
     CDS             17..541
                     /gene="ampD"
                     /locus_tag="VFP93_05295"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005502096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HET9844053.1"
                     /translation="MSNARYTFSPNQDERPKHCVLDTLVIHCISLPPGEFGGSSIEDL
                     FMNRLTIQAHPFYAMLKGLKVSAHLLIQRNGEIIQFVPFHKRAWHAGVSEFLGRTQCN
                     DFSIGIELEGTDSSPFEIAQYESLIACTLAICKSYNKITKDRIVGHSEVAPGRKTDPG
                     PFFNWDFFKNHLNL"
     gene            658..3321
                     /gene="aceE"
                     /locus_tag="VFP93_05300"
     CDS             658..3321
                     /gene="aceE"
                     /locus_tag="VFP93_05300"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET9844054.1"
                     /translation="MVHESQNKDIDPIETQEWLDALDSIVHVEGPQRAKFIIEQLLGK
                     AQLAGFSTDLCRNTPYCNTIHPEQEPQMPGDSALEQRSRALTRWNALAMVVRAQKVDP
                     SIGGHIGTFASSATLFDVGLNHFWKSPKEEGGGDLIYFQGHASPGMYARSFLLGDLTE
                     EQLVHFRRETDKKGLSSYPHPWLMPDYWQFPTVSMGLGPLQAIYQARFMKYLEHRGLS
                     NNPDRKVWCFCGDGEMDEPESTGALSIASREKLDNLIFVINCNLQRLDGPVRGNGKII
                     QELEAIFKGAGWEVIKVVWGDLWDPLFARDKSGKLLQRMEEAVDGDYQNYKAKGGAYT
                     REHFFGIYPETQKLVAEMSDEEIWHLNRGGHDPKKVYAAYAKAIKTKGKPTVILAKTI
                     KGYGLGKAGEALNIAHNVKKIDAEDIMAFRDRFNIPIRDEDIEKVPFYRPSEDSPEIR
                     YLRKLREKLGGPLPFRRRKASEAILPIPDLAIFDNQLKSTGDREISTTMAFVRILSAL
                     IKDKSLSKRIVPIVPDEARTFGMEGLFRQLGIYAPEGQKYEPVDKDQVMFYKEMESGQ
                     ILEEGITEAGSFCSWLAAASAYSSHNLVMIPFFIFYSMFGFQRIGDLAWAAGDMRSRG
                     FLLGGTSGRTTLNGEGLQHQDGHSHIMAGLVPNCISYDPTFAYELAVIIHHGLQRMIS
                     NEEDVFFYITLMNENYAHPDMPQGVEEGIIRGMYLFKKDEEHTLKVQLLGSGAIFQEV
                     IEAAHLLKKDFQVSADIWSVTSFNELQRNGREVARHNLLNPDAKQTSYVETCMKDTKG
                     PVIAATDYVKLYAEQIRGFIPRRYICLGTDGFGRSDTRTTLRNHFEVDRFHIAYAAIQ
                     SLAEDGHLDQNVVKKAKQLYQIHDKPDPAYC"
     gene            3334..>3701
                     /locus_tag="VFP93_05305"
     CDS             3334..>3701
                     /locus_tag="VFP93_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9844055.1"
                     /translation="MTLSVTEIKVPNIGDFNDVEIIDVLVEPGQTIEREQSLITLETD
                     KAAMDVPSPQAGTIQELKVKKGDKVSQGSVILLLEAQKQQIKQDNPEKNAEEVQTASV
                     GVSESESHTELESEDLVASET"
BASE COUNT         1128 a          682 c          839 g         1052 t
ORIGIN      
        1 ttcaagccag catttaattt cgaatgcgcg ctatacattt tcgccaaatc aagatgagcg
       61 tccaaagcat tgtgtgctcg atacccttgt tattcattgc ataagtttac ctccgggaga
      121 gttcggtgga tcttcgatag aagatttatt tatgaatcgt ttaactattc aggcacatcc
      181 tttttatgcg atgttaaaag gtttaaaagt atccgcacat ttattgattc aacgtaatgg
      241 cgaaattata caatttgttc cttttcacaa acgggcttgg catgctggcg tatcagaatt
      301 tttgggtcga acccaatgta atgatttttc cataggtatt gaattagaag ggacggattc
      361 cagcccattt gaaattgctc aatatgaaag tttaatagct tgtactctgg ctatttgtaa
      421 gtcttacaac aaaataacta aggatcgtat tgtaggacat agtgaagtgg ctcccggaag
      481 aaaaaccgat ccagggcctt tttttaattg ggattttttt aaaaaccatt taaatttgta
      541 gcgcgatcaa ataagttaga caagtaaagt agttgatcaa ttcattttgt ggtgcctcaa
      601 cacttttcgt aagcatggta taatcgcgcc aaactgaatg ttctcaggaa tgatttcatg
      661 gttcatgaaa gccaaaataa agacattgat cctattgaaa cacaagaatg gcttgatgct
      721 ttagattcaa ttgtacatgt tgaaggccca caacgtgcta agttcatcat agaacaatta
      781 ctagggaaag cgcaattagc tggattttct acggatttgt gtagaaatac tccttattgt
      841 aatacgattc atccagaaca agaaccacaa atgcccggcg atagtgcttt ggagcaacgc
      901 agtcgcgcac taacacgatg gaatgctttg gcaatggttg tccgtgccca aaaagtggat
      961 ccaagtattg gcggccatat tggtacgttt gcatctagcg ccacgttatt cgatgtggga
     1021 ttaaatcatt tttggaaaag tcccaaggag gaagggggcg gagatctgat ttatttccaa
     1081 gggcatgctt ctcctggtat gtacgctcgc agttttttat taggtgattt aaccgaggaa
     1141 caattagtac atttccgacg cgaaaccgat aaaaaaggcc tgtcttctta tcctcaccca
     1201 tggttgatgc ctgattattg gcaatttcca accgtttcca tggggctagg tccattgcaa
     1261 gccatctacc aagcgcgttt tatgaagtat ctggagcacc ggggtttatc caacaatccc
     1321 gatcgtaaag tctggtgttt ttgtggcgac ggcgaaatgg acgaaccaga atcgaccggc
     1381 gctttatcga ttgcatcccg cgaaaagttg gacaatctta tttttgtaat caattgtaat
     1441 cttcagcgtt tggatggtcc cgtacggggt aatggcaaaa ttatccaaga attagaagcc
     1501 atttttaaag gtgctggttg ggaagttatt aaagtcgtat ggggtgatct gtgggatcct
     1561 ttgtttgctc gagataaatc gggcaaatta ttgcaacgta tggaagaagc ggttgacggc
     1621 gattatcaaa attacaaagc taaaggtgga gcctataccc gagagcactt ttttggcata
     1681 tatccggaaa cccaaaaact tgtggccgaa atgagcgatg aagaaatttg gcatttaaat
     1741 cgcggtgggc atgatccgaa aaaagtgtat gcggcttatg ctaaagctat aaaaaccaaa
     1801 ggaaaaccaa cggttatttt agcaaaaacc atcaaaggtt atggtttagg caaagcagga
     1861 gaagcactca atattgcgca taatgtaaaa aaaattgacg cggaagatat tatggcgttt
     1921 cgggatcgct ttaatattcc tatacgggat gaagatattg agaaagttcc tttttatcgc
     1981 cccagtgaag atagtcctga aattcgctac ttacgaaaat tacgcgaaaa gctaggcggg
     2041 ccacttccct ttagacgtcg aaaagcatcg gaagcgatct tacccattcc tgatctcgct
     2101 atttttgaca atcaattaaa aagcacaggc gatcgagaaa tctcgaccac tatggcattt
     2161 gtgcgtattt tatctgctct tattaaagac aaatcgctga gcaaacgtat tgtaccgata
     2221 gttccagatg aagcgcgtac gtttggcatg gaagggttat ttcgccaatt gggcatttat
     2281 gcgcctgaag ggcaaaaata tgagccggta gacaaagatc aagtaatgtt ttacaaagaa
     2341 atggaatccg ggcaaattct tgaagaaggt attaccgaag cgggttcctt ttgttcgtgg
     2401 cttgccgcag cgagtgcata tagttcacac aacttagtca tgattccctt ctttattttt
     2461 tattccatgt ttggttttca acgtattgga gatctagcgt gggcagcagg tgacatgcga
     2521 tcgagaggct ttttgttagg cggtacctcg ggacgcacaa cattaaacgg ggaaggttta
     2581 cagcaccaag atggtcatag tcacattatg gcaggccttg tccctaattg tattagctat
     2641 gatcctacgt ttgcttatga attagccgtg attattcatc atggtttaca gagaatgatt
     2701 agcaacgaag aagatgtttt cttttacatc actttgatga atgaaaatta tgcgcacccg
     2761 gatatgccac aaggtgtaga ggaagggatc atacgtggta tgtatttatt taaaaaagac
     2821 gaggaacaca cacttaaggt gcaactttta ggcagtggcg caatctttca agaagtaatc
     2881 gaagcagcgc acttgttaaa aaaagatttt caagttagtg ccgatatttg gagtgtaacg
     2941 agctttaatg aattgcaacg caacggtcga gaagttgcaa gacataattt attgaacccc
     3001 gatgcaaagc aaacctctta tgtggaaacc tgtatgaaag acactaaggg gccggttatt
     3061 gcggctacag actatgtaaa attgtatgca gaacaaattc gtggctttat tccccgacgt
     3121 tatatttgtt taggcacaga cggctttggt agaagtgata cccgtacaac gttgcgcaac
     3181 catttcgaag tcgatcgatt ccatattgct tatgctgcga tacaaagttt ggccgaagat
     3241 gggcatttgg atcaaaatgt ggtgaaaaag gcgaagcagt tatatcaaat acatgataaa
     3301 ccagatccgg cttattgtta attgattggg agaatgacct tgtccgtaac agaaatcaaa
     3361 gttcccaata ttggtgactt caatgatgtc gaaattattg atgttctagt agagccggga
     3421 caaaccatcg aaagagaaca atcccttatt acgcttgaaa cagataaagc ggcaatggat
     3481 gtacctagcc cacaagcagg tacgatacaa gagctgaagg tgaaaaaagg tgataaagta
     3541 tcgcaaggct ccgttatttt attgctggag gcacaaaagc agcaaataaa acaggataat
     3601 ccagaaaaaa atgctgaaga agtacaaact gcttccgttg gcgtatcaga atctgaatcc
     3661 catactgagt tagaatctga agatcttgtc gcttctgaaa c
//
LOCUS       DASTIB010000304         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1110782, whole genome shotgun sequence.
ACCESSION   DASTIB010000304 DASTIB010000000
VERSION     DASTIB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110782"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>2401
                     /locus_tag="VFP93_05310"
     CDS             53..>2401
                     /locus_tag="VFP93_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9844056.1"
                     /translation="MELDYIFRILIDHGADKEETFMNMTPLHYAIYKKIESLACILVE
                     NGANLDAMYNDQTPLHHAIATQCTTVVKKLLESKVDTERLYNGMTPLHHAIKQKQSHI
                     VELLLHKNIDTEFHYNGMSPLHHAIKKGDTKSVKLLLDFKVNYESEYHGMTPLHHAIQ
                     SNCADIAEFLIDFGVDLERKYKGLTPLAHAIAKNSYFIAALLLEDNVNSEGIPDIIVA
                     IIANYIEKFEDCLSKKESIGEKYENKLPIHFAIICNRPTMLQRLIKSGASIESKYKDK
                     SPLHLAISYRNLDVVNILLEAGANTESTYKRKTALQYAVVKQTKEIAFALLKHGANKD
                     TPFEILKNANSKILSILKPYCTIFGVPYRFIFNNIKSENIDLDAFLAADDPILFLEEH
                     NYSTEVTLQALRLLNGANVIDICENNRRLDEENVMEDSKVLRANHFFKNTIQPHFKNT
                     LLSYSKNHDYEEALNTISTAIKELILDNVLAEAKDHNNSTLIHFIISNRDKLIQSDES
                     VLKESIPIFSCANSSTQAAWRGFNPFAPVAGDWPNLLIKAQESTTIFTGIAHTEDLDL
                     RTAYEILRERIAYYYLAVIDPHDGDDATRATRVQNFIQQLYSIRNAHGLDDPSCFPGC
                     VTRIADMGAFHSVAQLPITIKDWARNYFTEKALGVFRASLEELGSEETKQNLLNALIT
                     MTTDNAQSIIVKPENYNFSLLALRHEFIAKLGTAEENFDFISHNNLYPIVEQDQIYIE
                     HTINDIGSGGTGIALAEQFDKYIDKVPTSEDIACANPFSPVHN"
BASE COUNT          851 a          402 c          414 g          734 t
ORIGIN      
        1 aactcctcta aatagaacta tttacgatta taatttcgat gattgcattt caatggaatt
       61 ggactacata tttaggatat taatcgacca tggagcggat aaagaagaaa ctttcatgaa
      121 tatgacaccg cttcattatg caatttataa aaaaattgaa agtctagctt gtatccttgt
      181 tgagaatggt gcaaatttag acgctatgta taatgatcaa actcctttac atcatgctat
      241 tgctacccaa tgtaccactg tagtcaaaaa attacttgag tcaaaagttg atacagaaag
      301 actctataat ggtatgacac cactacatca tgctattaaa cagaaacaat ctcacattgt
      361 ggaattgttg ttacataaaa atattgatac agaatttcat tataacggca tgagtccatt
      421 acaccacgca ataaaaaaag gagataccaa aagcgttaaa ttattattag attttaaagt
      481 aaattatgaa tctgaatatc acggcatgac accgctgcat catgcaatac aaagcaattg
      541 tgctgatatt gcagaattct taatagattt tggagttgat ctagaaagaa agtataaggg
      601 attaaccccg cttgcgcatg ctattgctaa aaattcctat ttcattgcag ctttattatt
      661 agaggacaat gtaaattcag aaggtattcc tgatataatt gttgcaatta ttgcaaatta
      721 catagaaaaa tttgaagact gtttaagtaa gaaggaaagt ataggggaaa aatatgaaaa
      781 caaattgccc atacattttg ccattatctg caatcgaccg actatgttac aaaggttaat
      841 taaatctggt gcatcgatcg aaagtaagta caaagataaa tcccccttgc atttggcaat
      901 ttcttatcgc aatttagatg tcgtaaatat tcttttagaa gcaggcgcca atacagaatc
      961 aacttataaa aggaaaacag cacttcaata tgcggtagta aaacaaacaa aggaaattgc
     1021 attcgcctta ctcaaacatg gtgccaataa agatacaccc tttgaaattt taaaaaacgc
     1081 taattccaaa atattaagca ttttaaaacc ttattgtaca atttttggtg taccttatcg
     1141 gtttatattt aataatatta aatctgaaaa catagactta gacgcatttt tagcggcaga
     1201 tgatcccatc ctttttttag aagaacataa ttacagcaca gaagtcaccc tccaggcact
     1261 gcgtttgctc aatggagcta atgtgattga tatttgtgaa aataatcggc gtttagatga
     1321 agaaaatgtt atggaagatt ctaaagtttt gcgtgcgaat cactttttta aaaacacgat
     1381 ccaaccgcat tttaaaaata cattgttaag ttattcgaaa aatcatgatt atgaagaagc
     1441 tttaaatact atctctactg ctattaaaga gttaatttta gataatgtat tagcagaagc
     1501 taaagatcat aataattcaa ctctaattca ttttataatt tctaatagag ataaacttat
     1561 ccaatcggat gaaagtgttt taaaagaatc tatacctata ttttcttgcg caaactctag
     1621 cacacaagcc gcatggcgcg gatttaatcc ttttgcacct gtggcaggag attggccaaa
     1681 tttgttaata aaagctcagg aaagcactac aatttttact ggaattgcgc atactgaaga
     1741 cttagacttg cgtactgcgt atgaaatttt gcgcgaaaga attgcctatt attatttggc
     1801 tgttattgat ccccacgatg gggatgatgc gacacgagcc acgcgtgtgc aaaattttat
     1861 tcagcagcta tattccatac gtaatgcaca cggtttggat gatcccagtt gttttccagg
     1921 atgtgtaaca cgcattgccg atatgggtgc gtttcattct gtagcgcaat tgccgatcac
     1981 tattaaagat tgggcacgaa attattttac agaaaaagca ttaggtgtat ttcgcgcgtc
     2041 actcgaagaa ttaggttctg aggaaacaaa gcaaaattta cttaacgcat taataactat
     2101 gaccacagac aatgcgcaaa gtattatagt aaaacccgag aattataatt tttctttgct
     2161 ggcgttgcgg catgaattca ttgcgaagtt gggtaccgct gaagaaaatt ttgattttat
     2221 atcacacaat aatttatatc ccattgttga acaggatcaa atatatatag aacatactat
     2281 aaatgacata ggatcgggag gcactggaat agcattagca gaacaattcg ataaatatat
     2341 tgacaaagta ccaacatccg aggatatagc ttgcgcgaat ccattttcac cagtacacaa
     2401 t
//
LOCUS       DASTIB010000305         4674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_857463, whole genome shotgun sequence.
ACCESSION   DASTIB010000305 DASTIB010000000
VERSION     DASTIB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857463"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3841)
                     /locus_tag="VFP93_05315"
     CDS             complement(<1..3841)
                     /locus_tag="VFP93_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9844057.1"
                     /translation="MPKFHPALLKEYYLTHVNFLKNLEFSENTRPPIENIVFEGGGTR
                     GVIYAGVIETLEAYQILPNVQRVAGSSAGAIAATLVAVGFTGAELTKAFTEEINFKDF
                     MDHRSKWDPKFIKIQGFRLGISSFFNLFKRFGIYKGDAFKKTLGKLIKNKIERKLEEF
                     IRTNYSPESPHAILSPEFIEKKLAEIRDQFYIQDFKYITFGQLHALKEAFPTLELKDL
                     YLTGTNLTDASLKVFSFEHHSNMSILEAMRISMSFPLGFEPVYYQGHYYSDGGIANNY
                     PMSIFDKEPFLSHGLNDAGANPCTLGFLVDTKEEIKKRWGILDQVPSSLSFINFISNV
                     LSCIEYKMDIVKHKYALNSVQIRDKDIQGLDLEISPQQIQRGINAGKKYAQEYIQLYL
                     GQDVLYQEKNYDDLYQKYFSMNKTQLLKSYDDLIESLLNIETLQIKLKKIIDQNLSQK
                     LERLVMNFSPELVQEQEALFNHAQNLHESLAKILEDITAIQIRKANKELQQSSIIKKI
                     QTLIQLLNNSRSEDRSWLNEEIIKLYKNFEHSQNELNEISQELQEHEKNVHNISTTLN
                     TIDRKKNRIIYDLLFQIKTLNYIQEHEVLSDLARINEIYHEHFDILLDALEAKREKSD
                     DPRINPTSEMSSIVSALDTATSSPKNLAFNHFLSLRKLTKKLKKESDADFIQYENAQD
                     LTANATKFKQQGFGKKISDYDVATIKIKTKEKMHWKSYTRSEKASMILAHILVPSKEA
                     LANANKATKDILIVFPSTVSSGQSNFSSLQKNYSAHNKVFEENRERFLTQLIWAIRQI
                     KSFSEPKDAKIQLTIFGEGIGGLDAQLMLIDILKEYESLETLRALTNIELILQDAPKV
                     QERQAIEFSQTINKLKITHKHPALSIEHYKHKNYTKGLTASNYFGECDLANFTQENDL
                     ACRAHVLSPRGLPIKQFIKNKDSKEYDSFLNISQSHFKQQILQNVSSFIKNNKKMTNY
                     VRKHFLSELKNIAYHGAKNGLLVPYSFLKIPYYTGRRLIAEIKSWFRSEQITPIPNWI
                     AQPQRVEFESSYSIQDEAFDRFMRSTTLEQRLSRGEKPPVENLVLAGSGLNSLTYIGA
                     LNSLSKSGILKTIKRIAATSWGGFIAGLTAIGFHPSEITQVIEQLKLSKVYDKSLFFD
                     PPFFKVREHLIGIGDLISLFKNKGLYKGEVFTHMMQSLIQNKLLENLKKMVWDQITPE
                     MLALVSTQVLDPDKLNIFMDKYLAWEVKKILAKLHITNLGEITFGQLKQLSELYPQLQ
                     IKELYISATNLEQGGLTI"
     gene            complement(3921..>4674)
                     /locus_tag="VFP93_05320"
     CDS             complement(3921..>4674)
                     /locus_tag="VFP93_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9844058.1"
                     /translation="AKEAKNPQVSYPLSMLVSSGIILFIYIFGSLSIAFVVSPRDINL
                     FAGLMQAFTEFFTAFNLRPILPIVVFLAVVGSLAGINTWIIGPAKGILASAHYGFLPP
                     WMQKTNKKGMPIGTLFVQAIIASLLSMVFYFMPSVNAAFWIITALTVQFAMIMYILIF
                     CACIKLRYSQPDQYRHYRIPGGKLGVWLVAGVGSLASSFAFFICYVPPSQIDTGNIWF
                     FESFLLLGLIGLSLPPIYFIFFRKAHWQLKAL"
BASE COUNT         1442 a          820 c          790 g         1622 t
ORIGIN      
        1 agattgtaag accaccttgt tctaaattgg ttgccgaaat atagagttct ttaatttgca
       61 attgcggata taactcactt aactgcttta attgcccaaa agtaatctcc ccaagattag
      121 ttatatgtaa tttagctaaa atcttcttta cttcccacgc taaatattta tccataaaaa
      181 tatttaactt atctggatcc aaaacttgcg tgcttacaag ggctagcatt tcgggtgtga
      241 tttggtccca caccattttt tttaagtttt ccaacagttt gttttggata agggattgca
      301 tcatgtgcgt aaaaacttca cctttatata aacccttatt tttaaataag gaaattaaat
      361 ctcctatgcc aattaaatgt tctcgtactt taaaaaaagg aggatcaaaa aaaagtgatt
      421 tatcataaac tttagaaagc ttaagttgtt cgatgacttg cgtaatttca gagggatgaa
      481 aaccaatagc cgttaaaccc gcaataaatc ctccccatga agtggcagca atgcgcttta
      541 tcgtttttaa tattccggat ttgctcaacg agttaagtgc cccaatgtaa gtaagactat
      601 ttaaacctga tcctgctaac actaaatttt ctacaggagg cttttcaccg cgactcaatc
      661 tttgttccaa ggttgtggat cgcataaacc gatcgaaagc ttcgtcttga atagaatagg
      721 agctttcaaa ttccacgcgt tggggttgtg ctatccaatt gggaattggg gtgatctgct
      781 ctgatcgaaa ccaggatttt atttctgcta tcagacgacg tcccgtataa taaggaattt
      841 taagaaacga ataaggtacc aaaagaccat tttttgcacc gtgataggca atattcttta
      901 gttcagataa aaaatgcttt cgaacatagt tagtcatttt tttattattt ttaatgaaac
      961 tcgaaacatt ttgtaaaatt tgttgtttaa aatgcgattg agaaatattg agaaaagaat
     1021 catattcctt ggaatcttta ttttttataa attgcttgat aggcaatcca cgtggtgata
     1081 gtacgtgtgc acgacatgcc aaatcatttt cttgcgtaaa atttgctaga tcacactctc
     1141 caaaataatt gctggctgtt aatccttttg tataattctt gtgtttataa tgctcaatag
     1201 ataatgcagg atgtttatgt gtaattttta atttgttaat ggtttgtgaa aactcaatag
     1261 cttgtcgttc ttgaactttc ggcgcatcct gaagtatcaa ttcaatattg gttaatgcac
     1321 gcaatgtttc caaggattcg tactctttta aaatgtctat taacattaat tgtgcatcta
     1381 atccacctat gccttcacca aatattgtca attgtatttt tgcatccttc ggttctgaaa
     1441 acgacttaat ttgtcgaatt gcccaaatca attgggttaa aaaacgctct ctattttctt
     1501 caaaaacctt attatgtgcg ctataatttt tttgtaaaga gctaaaatta ctttgacccg
     1561 aagataccgt ggatgggaat actattaaga tatcttttgt cgctttatta gcattcgcta
     1621 aagcttcctt agagggaact aatatgtggg ctagaatcat tgatgccttt tctgatcgcg
     1681 tatagctttt ccaatgcatc ttttcttttg ttttgatctt tattgttgcg acatcgtaat
     1741 cagaaatttt ttttccaaaa ccctgttgtt taaactttgt tgcattagct gtaagatctt
     1801 gtgcattttc atattgaata aagtcggcat cactttcttt tttaagtttt tttgttaatt
     1861 ttctcagtga aagaaaatga ttaaatgcta aatttttcgg tgaactcgtt gccgtatcga
     1921 gtgcactaac gatagaggac atttctgatg ttggatttat tctcggatca tccgatttct
     1981 ctctttttgc ctccagcgca tccagtagaa tatcaaaatg ttcgtggtat atttcattta
     2041 ttctagctaa atcggataag acttcatgct cttgaatata attcaaagtt ttgatttgaa
     2101 ataacaaatc gtatattatt ctattttttt tcctatcaat agtgtttaat gtcgtcgaaa
     2161 tattgtgcac gtttttttca tgctcttgca attcttgtga aatttcgtta agttcatttt
     2221 gagagtgttc gaagttttta tataatttaa taatttcttc atttaaccat gatctatcct
     2281 cagatcggga gttatttaag agttgaatta acgtttgaat tttcttaatt atggaacttt
     2341 gttgtaattc tttattggcc ttacgtatct gtattgctgt gatatcttca agaattttag
     2401 ctaaagattc gtgcaaattt tgtgcatggt taaacagtgc ttcttgttct tgaactaatt
     2461 caggtgaaaa attcataacc aatcgctcta gtttctggct taaattttgg tcgataattt
     2521 tttttagttt gatttgtaaa gtttctatat tcagcaacga ctcgattaaa tcatcatagg
     2581 attttaacaa ttgtgttttg ttcatggaaa aatatttttg atataagtcg tcataatttt
     2641 tttcttgata cagtacatct tgtccaagat aaagttgaat atattcttgt gcgtattttt
     2701 ttcctgcgtt aataccacgc tgaatttgtt gcggactaat ttctagatcc aggccttgga
     2761 tgtctttgtc tcgaatttgc acggaattta aagcatattt atgcttcaca atatccattt
     2821 tgtattcaat acagcttaga acattgctaa taaaattaat aaaacttaag ctagatggta
     2881 cctgatctag gattccccat cgttttttga tttcttcttt tgtgtcgact aaaaagccta
     2941 acgtgcaagg attagcacct gcatcgttca aaccatgaga caaaaagggt tctttgtcaa
     3001 aaatactcat cggataatta ttggcaattc ctccatcgga ataataatgt ccctgatagt
     3061 aaacaggttc gaagcctaac ggaaagctca tcgaaattcg cattgcttcc aatatagaca
     3121 tgttagaatg atgttcaaaa gaaaatactt tcaaacttgc atcggtgaga ttagtacccg
     3181 tcaagtataa gtctttgagc tctaacgtgg gaaaagcttc ttttaacgcg tgtagttggc
     3241 cgaaggtaat gtatttgaaa tcctgaatgt aaaattgatc acgtatttct gctaactttt
     3301 tttcgatgaa ctcaggtgac agtattgcat gaggagactc tggactatag tttgtacgta
     3361 tgaattcttc caatttgcgt tcaattttat tttttattaa tttacccaat gtttttttaa
     3421 acgcatcccc tttataaatt ccaaagcgct taaataggtt aaaaaaactg gagattccta
     3481 atctgaaacc ttgaattttt atgaatttag gatcccattt acttctatga tccataaaat
     3541 ctttaaaatt tatttcttct gtaaaagctt ttgttaattc tgctccggtg aagccaacgg
     3601 ctactaaggt agcggctata gcaccagctg aagagcctgc tactctttgc acattgggaa
     3661 gaatttgata ggcttcaagt gtttctatga cacccgcgta aatgacacct cttgtcccac
     3721 ccccctcaaa cacaatattt tcaatgggtg gacgtgtatt ctcagaaaat tcaagatttt
     3781 ttaaaaaatt tacatgggtt aaataatatt cttttaataa cgctggatga aatttgggca
     3841 taaatccgtg taattgccac aatgcaaata actaacttaa ttctaagata tcataatctc
     3901 acccaacgcg aatactgtac ttacagagcc tttagctgcc aatgcgcttt tctgaaaaat
     3961 ataaagtaaa tgggcgggag acttaaccca attaaaccca atagtaaaaa gctttcaaaa
     4021 aaccaaatat tgcctgtatc tatttgtgag ggtgggacat agcaaataaa aaaagcaaaa
     4081 ctagaagcca aactcccgac ccccgctact aaccacaccc ctaatttacc tccgggaatt
     4141 cgatagtgtc ggtattgatc aggttgtgaa tagcgtaatt taatgcacgc gcaaaatatt
     4201 aaaatgtaca tgatcatagc aaactgcacc gtcaatgccg taatgatcca aaatgcagca
     4261 ttcacgcttg gcatgaaata aaataccata gaaagtaagg aagcaataat cgcctgcaca
     4321 aataatgttc caataggcat acctttttta ttggtttttt gcatccaagg aggtagaaac
     4381 ccgtaatgtg cagaagctaa aattccctta gcaggtccta tgatccaagt atttattccc
     4441 gcaagagagc caacaacggc aagaaatacc acgataggta aaatgggtct taagttaaag
     4501 gccgtaaaaa attcagtgaa agcttgcatt aagccagcaa aaagatttat atctcgaggc
     4561 gaaaccacaa aggcaatact taaagatcca aagatgtata taaataagat aatgccagat
     4621 gacaccagca tagataaagg ataactgacc tgaggatttt tggcttcttt cgca
//
LOCUS       DASTIB010000306         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_367763, whole genome shotgun sequence.
ACCESSION   DASTIB010000306 DASTIB010000000
VERSION     DASTIB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367763"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..396
                     /locus_tag="VFP93_05325"
     CDS             79..396
                     /locus_tag="VFP93_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844059.1"
                     /translation="MPSDVIIIGTITEAITNYSELIYLYFWHDKHGTITGCYECEAKY
                     YDTVDEIYSHPKLMDNVRWLLTECQNRRLLISFRNLHIFILVLLIMVVTTIELSTWEV
                     KCL"
     gene            387..934
                     /locus_tag="VFP93_05330"
                     /pseudo
     CDS             387..934
                     /locus_tag="VFP93_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            988..1164
                     /locus_tag="VFP93_05335"
     CDS             988..1164
                     /locus_tag="VFP93_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844060.1"
                     /translation="MHLDEETWARARGWAMWKALYEILVLEDKSGAALAKQQQIIDAV
                     MKEDKSSPKTSREQ"
     gene            1368..1901
                     /locus_tag="VFP93_05340"
     CDS             1368..1901
                     /locus_tag="VFP93_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844061.1"
                     /translation="MKFEELEPAEVPLYNPALYHNIRQAIEQEVNKSPNKIIYSIAIH
                     LSFCVQNLHKNMEKRLRDLLEYHNSHNGKLPSEIYFEPSRPNIFPPPPKKPDDLPHLN
                     KLLVHHANFYPMKSMRVTWAYLVTQYLETLAFSIENKRLNECLQNGQDGCFDKCISDM
                     GEWKTLQTSMATAKPGL"
BASE COUNT          704 a          438 c          508 g          636 t
ORIGIN      
        1 gggggcgaga tagcgactag tgaaacgcca atagaagctt tagtccgtga attacatgaa
       61 tagttaggtg caaaagtaat gccgtcagat gtgattatta taggaaccat tacggaagca
      121 atcacaaatt acagtgaatt gatttatctt tatttttggc atgataagca tggcactatt
      181 actggctgct atgaatgcga agcaaaatac tacgatacag ttgatgaaat ttattcccat
      241 ccaaagctga tggataatgt gcgatggttg ttgacggaat gccaaaatag aagattgctt
      301 atcagtttcc gcaatttgca catcttcata ttagttctgt taatcatggt ggtaacgaca
      361 atagaacttt ccacctggga agtgaaatgt ctataagatt accaagtgcg caagaatatg
      421 aaagacaggt gcaaaaagaa caaacatggc tgcctaagat cgcgccattc ttaccacttc
      481 ctatccctcc gcctattgct aaggggaagc ctacagaaat ctatccttgg agttggtcga
      541 tatacaaatg gatagaggag agaccgcaaa tagcctagac cttagtgatg cgcatttaga
      601 gacaatcgcc gcgcagcttg cgcaattctt aactaagttt cataagtttg aagctgcagg
      661 cgcttctgtt ccgggacttc ttaactggtg gcgtgcggct catgccttag tttatgatgc
      721 gcagacgaag tcactcattg aagcactcaa agattttgtt gatgttggta acgcaagatc
      781 gctttggcaa agagccataa attctaaatg gaatagagat ccagtatggg ttcatggaga
      841 tgttgcgagc ggtaatctct tggtaaaaga taataggctt gttgctgtaa ttgactttgg
      901 ttgcatggga attgggatcc agcctgcgat ttaacaatag cttggacatt ttttagaggc
      961 gagagtagaa agatttttaa ggcgaatatg catttagatg aggagacttg ggcaagggca
     1021 cgtggttggg ctatgtggaa ggcactttat gagattttgg tgcttgagga taaaagcggt
     1081 gcagctttag cgaagcaaca gcagattatt gatgctgtta tgaaggagga taaatcaagc
     1141 cccaaaacga gtcgtgagca atgaatactg gaggagtgaa gtttcgccag ctcgttggag
     1201 atgggaatat acattaaatt tggaacccat aactataaaa aaaaacatta atcgataaaa
     1261 aggcttgttc atcaacaaaa agaatacgat tggaattttt ggcttgaaaa tatagttttc
     1321 aaactttaaa atgcaatttt tactttcccc gcagagcaat taaagttatg aaatttgagg
     1381 aattagaacc tgctgaggtt ccgctttata atcccgcctt gtatcataat atccgtcaag
     1441 cgattgaaca ggaagtcaat aaaagcccta ataaaataat ctattcaata gcaatccatt
     1501 tgtccttttg cgtgcagaat ctgcacaaaa acatggagaa acggttgagg gacttgttgg
     1561 aataccacaa ttctcataac ggtaaacttc cctcggaaat ttattttgaa ccctctcgcc
     1621 ccaatatatt cccgccacca cccaagaagc ctgatgattt accgcatttg aataagcttt
     1681 tagtacatca cgcaaatttt tatccgatga aatccatgcg ggttacttgg gcatacctag
     1741 tcacccaata cctggaaacc ctagcttttt ccattgaaaa taagcgattg aatgaatgtc
     1801 tccagaatgg ccaggatggc tgtttcgaca agtgcatcag cgatatgggt gagtggaaaa
     1861 ctttgcaaac cagcatggct actgcaaaac caggtttata aaatattagg cgggcttcca
     1921 tagttttctg gaatgcccat tttttgttgc cgcttccgaa tcttccccgc gaatggaaag
     1981 caaaaaaagg taattttatt ttcgcgcggg cgtccacagc ggcttaagaa acgcttgaac
     2041 ttgatcttgt gtaccgatcc ccgcgagcca atttcccaga ttatttcacg aggcagcgaa
     2101 aggacttggg gaatcaaaaa gaggttactc cccagaaaca attagagagc gcagcctttt
     2161 ttaaaacagt taaccctact tggccaaaac ttgcaaacca gcatagctac tgcaaaacca
     2221 ggtttataaa attttaggcg ggcttccata gttttcttga tgcacatttt ttgttgccgc
     2281 ttccga
//
LOCUS       DASTIB010000307         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_776248, whole genome shotgun sequence.
ACCESSION   DASTIB010000307 DASTIB010000000
VERSION     DASTIB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776248"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFP93_05345"
     CDS             complement(<1..253)
                     /locus_tag="VFP93_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9844062.1"
                     /translation="MTHKILLLAGDGIGPEIMAQAKKILLTLQKNSALAFAWEEGLIG
                     GAAIEAFGNPLPDATIQRARNSDAILLACVGGPKWDNLPS"
     gene            complement(250..906)
                     /gene="leuD"
                     /locus_tag="VFP93_05350"
     CDS             complement(250..906)
                     /gene="leuD"
                     /locus_tag="VFP93_05350"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9844063.1"
                     /translation="MKPFTVHTGKVVPLMQANIDTDIIMPKQFLTSIERTGYGDFIFD
                     AWRYLDKGEPGMDCHNRPRNPNFPLNFTRYLGATILLTGPNFGCGSSREHAPWGLFEY
                     GFHVISASSFADIFYNNCIQNGILPIVLPQHIVESLCHEVEAQIGYLLTVDLAAQSLR
                     KPNKETLTFSIDSMQKNKLLSGKDSIEYILNFKEKIVDFEHRHRQEAPWLYNRLKEEK
                     "
     gene            complement(909..2306)
                     /gene="leuC"
                     /locus_tag="VFP93_05355"
     CDS             complement(909..2306)
                     /gene="leuC"
                     /locus_tag="VFP93_05355"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET9844064.1"
                     /translation="MIPQTLYDKIFANHVVHQMDDQTFLLYIDCQLLHEVTSAQAFAN
                     LRLKQRKLWSPTANIAVPDHNIPTIERFTIQDAIAKQQILKLTENCKENGILLFDLAD
                     HRQGIVHIIGPEQGITLPGMTIVCGDSHTSTHGALGTIAMGIGTSEVEQVLATQCLII
                     KKPKNMRVLFEGSMPRGVYAKDLILALIGKMGTQGGTGFAIEYSGGVISDFSVEERMT
                     LCNMSIEAGAPCGLTAVDETTLQYIKGKPFAPKEHLWQQAIQSWKKLKSDRGAYFDKE
                     VRFFVSDLAPQVTWGTSPDMVVGINQKIPDPEQEPNLIKREHMNRALKYMGLKPALPV
                     TQIPLEKIFIGSCTNARIEDLRVAASILKGHKISKKIKQALVVPGSGLIKKQAEEEGL
                     DQIFKESGFEWRDPGCSMCLGMNPDRLSPGEHCASTSNRNFESRQGLGGRTHLCSPAM
                     AAAASIAGHFIDIKE"
BASE COUNT          755 a          569 c          463 g          835 t
ORIGIN      
        1 cagaaggtag gttatcccat tttggacctc ccacgcatgc caatagaata gcatcactgt
       61 ttcgtgccct ttgtattgtt gcatctggta gcgggttacc aaaagcctca atggctgccc
      121 cgcctatcag tccttcttcc catgcaaacg caagagcaga atttttctgt aaagtaagca
      181 aaatcttctt tgcttgcgcc ataatttcag gaccaattcc atcacctgct aacaataaaa
      241 ttttatgggt catttttctt cctttaatcg gttgtacaac cagggagctt cctgtcggtg
      301 tctatgttca aaatctacga ttttttcttt aaaatttaaa atgtattcga tagaatcctt
      361 accgcttaat aatttgtttt tttgcataga atcgattgaa aaagtaagcg tttctttatt
      421 gggcttgcgc aaactttgtg ctgccaaatc aaccgtgaga aggtatccta tttgtgcttc
      481 aacttcatga caaagggatt cgactatgtg ttgaggtaag actatgggaa gtattccatt
      541 ttgaatacaa ttattataaa aaatatcagc aaaacttgat gcactaatga catgaaatcc
      601 atattcaaac agaccccagg gtgcatgttc cctagaagag ccacaaccaa aattaggccc
      661 ggttaataaa attgtagcac ccaagtatct tgtaaaattc aggggaaaat ttggattgcg
      721 aggccgattg tgacaatcca tacctggctc tcctttatct aagtatctcc atgcatcaaa
      781 aatgaaatcc ccatatcctg ttcgctctat ggatgttaaa aattgtttag gcataataat
      841 atcggtatca atattcgctt gcattaaagg tacgactttg ccggtatgta ctgtgaaagg
      901 tttcataact actccttaat atcgataaaa tgtcctgcaa tacttgctgc agctgccatt
      961 gcaggcgaac ataaatgcgt tctaccacct aaaccttgcc tactttcaaa atttctattg
     1021 gatgtggaag cgcaatgctc gccgggtgat aatcgatccg gattcatacc taaacacatt
     1081 gaacaacctg ggtcacgcca ttcaaaccca gattctttaa atatttgatc taagccttct
     1141 tcctccgctt gcttttttat taaccccgaa cccggaacca ccaacgcttg ttttattttt
     1201 ttggagattt tatgcccttt gagaatcgat gctgccacac gcaaatcttc tattctggca
     1261 ttggtacaag atccaatgaa tattttctct aaaggaattt gcgtcacagg taatgctggt
     1321 ttaaggccca tatattttaa ggcgcgattc atatgttcgc gctttatgag gttaggttct
     1381 tgttcaggat cgggtatttt ttgatttata cccacaacca tatctggaga tgtcccccaa
     1441 gttacttgag gggctaaatc gcttacaaag aaacgtactt ctttatcaaa ataggcccct
     1501 ctatcgcttt ttagcttttt ccaactttgg attgcttgct gccacaagtg ttccttaggt
     1561 gcaaacggtt tcccttttat atattgcaac gttgtctcat ccacagcagt taagccgcat
     1621 ggggctcctg cttcaatcga catattacat agcgtcatgc gctcttcgac actaaaatcg
     1681 cttataactc cacctgaata ttctattgca aaacctgtcc ctccctgagt acccattttt
     1741 cctatgagtg ctaaaataag atctttagca taaactccgc gtggcattga gccttcaaaa
     1801 agcacgcgca tatttttagg tttttttatt attaagcatt gcgtcgctaa tacttgttcc
     1861 acttcggacg ttccaattcc catcgctata gtaccaaggg caccatgggt agaagtatgt
     1921 gaatctccac aaacgatagt cattccaggt aaggtaatgc cttgttctgg tcctatgata
     1981 tggacaatgc cctgacgatg atcggctaaa tcaaacagta aaataccatt ttctttacaa
     2041 ttttcggtaa gcttcaaaat ttgttgtttg gctattgcat cttggatcgt aaaacgttct
     2101 atggtaggaa tattatggtc aggcacggca atatttgcgg tgggcgacca tagctttctt
     2161 tgttttaacc gcaaatttgc aaaagcttgc gcagaagtga cttcatgcaa aagttggcaa
     2221 tcgatataaa gtaaaaaagt ttgatcatcc atttggtgta cgacatgatt agcaaaaatt
     2281 ttatcgtata aggtctgtgg tatcattccc tgatccttct gcttaactgt tgcgtaacaa
     2341 aaccactaac atagtgacaa aaccaattaa aattagggcc aagataccca cggttgaaaa
     2401 actttttgtt aagttttctc tcgacataac ttccggattt gtcttaataa ctttgtaaat
     2461 tctccatacg ataaagatac acagtattaa aaaactaatt tgaacaaata gttgcataga
     2521 taaagccttc tcaaaaacac aaggtattat gcccagcctt gctcaaaaac ctcaacacct
     2581 tcgacctaac ccgattcatc tcctagtgct taagtccaat tt
//
LOCUS       DASTIB010000308         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_306552, whole genome shotgun sequence.
ACCESSION   DASTIB010000308 DASTIB010000000
VERSION     DASTIB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306552"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="mutL"
                     /locus_tag="VFP93_05360"
     CDS             <1..1172
                     /gene="mutL"
                     /locus_tag="VFP93_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9844065.1"
                     /translation="INDIFFNAPARRKFLKSSRTEFQHIERVVKNMALCNPQVQMRLV
                     HNDKQVRYYKKTHRKQRIQQIAGNHFLQNAVEMQVEHQNWKITGYVGNADALRKNADL
                     QYVFINNRCVKDKIIYHAIKNAFYEQNSLHNAFLSFILFLECPFDEIDINVHPTKAEV
                     RFHDPTWVHDFVQANVEKAYLASMPKTQAFCVSGINPTIYYKQEETKKEEERFCSSNT
                     IEPPITLMQFFWEHFYFEGGKDLYIIDLKKNQQAIWEHLIQERIHCPFPRNLILFPKK
                     LRLEYQSTLLLEKFGFTFRDNAKETSLLSEKPAFLHEEEMQRLLLNLTHATPATRERS
                     VYNFFKQLPWPVFLRHDFFKSRFSAWCKARSKHDLVVLMDPNTLQDWIALKIKAQ"
     gene            complement(1159..1401)
                     /locus_tag="VFP93_05365"
     CDS             complement(1159..1401)
                     /locus_tag="VFP93_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844066.1"
                     /translation="MSWEAVSWVLVVSSLIGNIFIIKKHVFGQWLWAVSDLGWIFYNI
                     HLGATAQAFLFFIYTTLCIWGIITWSRESNHPIIAP"
     gene            1526..2473
                     /gene="miaA"
                     /locus_tag="VFP93_05370"
     CDS             1526..2473
                     /gene="miaA"
                     /locus_tag="VFP93_05370"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET9844067.1"
                     /translation="MAQANHKSYKVICIMGPTASGKTNLAIELFCRVASMLISVDSAQ
                     VYRGLNIGAGKPSPKILDKVPHALINVVEPSQVYNAHQFCEDAKEIIKRANQEGQIPI
                     LVGGTMLYYRALLQGLSELPGANLLIREQLVQDLCVHGLSFLYEKLQFVDPVISQRIK
                     PNDSQRIQRALEVFYSSGKPLSVWQSEHKLSKTELNALCIGLLPEDHERPRLHQQITT
                     RFLKMMDQGLVEEVKNLLEDPKNSSELPAFKSVGYRQVVDYLIGTYDFQEMQEKAIIA
                     TRQLAKRQLTWLRSWPNLLRFNCFDSQLLKKVYKTICKF"
     gene            2637..3050
                     /locus_tag="VFP93_05375"
     CDS             2637..3050
                     /locus_tag="VFP93_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844068.1"
                     /translation="MEEFYKQLPASMQKNHTELAHTIALLKKFNFAKVRDKASAQNFS
                     DTYNSFFEALLELSKEMEALEEKNLELKKNWLKIQKEANHNLIELYSLESSINKYNSI
                     IERGVTSWINVLFVNKKAMPTLRIPIDYLMGEASL"
     gene            3166..>3362
                     /gene="hfq"
                     /locus_tag="VFP93_05380"
     CDS             3166..>3362
                     /gene="hfq"
                     /locus_tag="VFP93_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET9844069.1"
                     /translation="MPKGQSLQDPFLNALRKEKVSVSIYLVNGIKLQGQIESFDQFVI
                     LLKNSVNQMVYKHAISTIVPAR"
BASE COUNT         1177 a          573 c          598 g         1014 t
ORIGIN      
        1 aaattaatga catttttttc aatgcgcctg cgcgaagaaa atttttaaaa agctcacgaa
       61 cggaatttca gcatatcgaa cgggtggtta aaaatatggc attatgcaat ccacaagttc
      121 agatgcgact ggtgcacaat gacaagcaag ttcgatacta taaaaaaacg catcgaaagc
      181 aacgtattca gcaaattgca ggcaaccatt ttttgcaaaa tgctgtggaa atgcaggtag
      241 agcaccaaaa ttggaaaata acaggctatg taggaaacgc ggatgcctta agaaaaaatg
      301 ccgatttaca gtatgtattt atcaataata gatgcgtgaa agataaaata atttatcatg
      361 caataaaaaa tgcgttctat gaacaaaata gcttacataa cgctttttta agttttattc
      421 tttttttaga atgtccattc gatgaaattg atattaatgt ccatcccaca aaagcggaag
      481 tacggtttca tgatcccacg tgggttcatg attttgttca agcaaatgtc gaaaaagcgt
      541 atctggcatc catgcctaaa acgcaagctt tttgtgtttc tggtataaat ccgactatct
      601 attataaaca agaggaaaca aaaaaagagg aagaacggtt ttgttctagc aataccattg
      661 aaccaccgat aactttgatg caattttttt gggaacattt ttattttgaa ggaggcaaag
      721 atctttatat tatcgatttg aaaaaaaatc aacaggctat ttgggaacac ttaattcaag
      781 aaagaatcca ttgcccattt ccaagaaatt taattttatt tccaaaaaag ttgcgtttag
      841 aataccaaag tactctttta ttagaaaaat ttggttttac gtttagagat aatgcaaaag
      901 agacttcttt gctttctgaa aaacctgctt tcttgcacga agaggaaatg cagagattat
      961 tattaaatct tacccatgca actcccgcga cacgggaacg tagtgtttac aattttttta
     1021 aacaattgcc ctggcccgtt tttttgcgtc atgatttttt taaatcacgc ttctctgctt
     1081 ggtgtaaagc acgatcaaaa cacgatttgg ttgtgcttat ggatcccaat acgttacaag
     1141 attggatcgc tttgaaaatc aaggcgcaat aataggatga ttgctttctc tagaccaagt
     1201 gattatcccc caaatacaca aggtagtgta gataaaaaat aaaaaagctt gggctgttgc
     1261 tcctaaatga atattataaa aaatccagcc taagtcgctt acagcccaaa gccattgacc
     1321 aaacacatgt ttttttatga taaaaatatt gcctattagc gatgaaacca ctaaaaccca
     1381 actgacagct tcccaactca tacattattc cttataaaat tttttaatta agtcatacat
     1441 gcaaaattct aagaatgtga attgacttaa tcattctttc aacataatat gaattctaac
     1501 ttaatcgata aaatatatcc cagttatggc tcaagctaat cataagtctt ataaagttat
     1561 atgcatcatg gggcctactg cttctggtaa aacgaattta gccattgaat tattttgtcg
     1621 tgttgcaagt atgctgatta gtgttgactc agcacaagtg tatcgtggtt taaatattgg
     1681 cgcaggaaaa cccagcccta aaatattaga caaggtcccg catgcactta ttaatgttgt
     1741 tgaaccatcc caggtttata atgcgcatca attttgtgaa gatgcaaaag aaattattaa
     1801 aagagccaat caagaaggac agatcccaat tttggtgggt gggacaatgc tctattaccg
     1861 tgcgctactt cagggcttat cggaactacc gggtgctaac ttgttaatcc gggaacaact
     1921 ggttcaggat ctgtgtgttc atggtttatc ttttttgtac gaaaaattac aatttgttga
     1981 tccggtcatt tctcaacgaa ttaaaccaaa cgactcacaa agaatccagc gcgccttaga
     2041 agttttttat agctccggta agcctctttc agtttggcaa agcgaacata aacttagcaa
     2101 aaccgaattg aatgcattgt gcattggatt acttcccgaa gatcatgaac gtcctcgtct
     2161 ccatcagcaa attacaacgc gtttcttaaa gatgatggat caagggttag tcgaggaagt
     2221 taaaaattta ttagaggatc caaaaaattc ttcagagtta cctgcattca aatcggtagg
     2281 atatcgacaa gtggtggatt atttaatagg aacttacgat ttccaagaaa tgcaagaaaa
     2341 agcgatcata gcaactcgac aattggcaaa acgtcaatta acgtggttac gttcctggcc
     2401 caatttattg aggtttaatt gttttgattc ccaacttctc aaaaaggttt ataagacaat
     2461 ttgcaagttt tagaagtttt tttaaaacta aagctttttt taccctcgta attttgttct
     2521 taggcttcac acttatagca attttttcga cgcttaatgc aatcgatcat catttaaaaa
     2581 aaattcatgc aacaattgaa gatttatcgc ataagcaaga cgactactat agaaaaatgg
     2641 aagagtttta taaacaatta ccagcttcga tgcaaaaaaa ccatacagag ttagcacata
     2701 ctattgcact tttaaagaaa tttaattttg caaaagttcg cgataaagca agcgcccaaa
     2761 atttcagtga tacatataac tctttttttg aagcgttact ggagctttct aaagaaatgg
     2821 aagccttgga agaaaaaaat ctcgaactta aaaaaaattg gctaaaaata caaaaagaag
     2881 ccaatcacaa cttaatcgaa ctatatagtt tggaatctag cataaataaa tataatagta
     2941 ttattgagcg tggagtaaca agttggatta atgtgctttt tgtgaataaa aaagcaatgc
     3001 ccacattacg cattccgatt gattatttaa tgggagaagc gagtttatag ctgtctaaga
     3061 caggtagagt ttagtaagag catggaatat gctaaactaa gctggttatt agggaagatg
     3121 cctgtaaacc gaaaatagaa caagcagaat aacagggagg cttagatgcc aaaagggcaa
     3181 tcactacagg acccattttt gaacgcactt cgcaaagaaa aagtgtcagt ttcaatttat
     3241 ttagttaatg gaataaaatt acaagggcaa attgaatcat ttgatcaatt tgttatttta
     3301 ttaaaaaata gtgtaaacca gatggtttac aagcacgcta tttcaactat agttccagca
     3361 cg
//
LOCUS       DASTIB010000309         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1125659, whole genome shotgun sequence.
ACCESSION   DASTIB010000309 DASTIB010000000
VERSION     DASTIB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125659"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1592)
                     /gene="ahcY"
                     /locus_tag="VFP93_05385"
     CDS             complement(276..1592)
                     /gene="ahcY"
                     /locus_tag="VFP93_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9844070.1"
                     /translation="MQMTAKKSSSTQDYWIQDINLAAWGRKEVAIAEMEMPGLMALRK
                     EYSSTKPLKGAKIAGCLHMTVQTAVLMETLIALGAEIRWSSCNIFSTQDHAAAAMAAK
                     NIPVFAKKGETEEEFWWCIEQTLDGPDGWRPNMILDDGGDLTQLLHEKYPHYLKEIKG
                     ISEETTTGVQRLYERMREGKLLVPAINVNNSVTKSKFDNLYGCRESLIDGIKRATSVM
                     IAGKICVVLGFGDVGKGCAQAFRGLGATVWITEIDPICALQAAMEGYRVVSMDYAASL
                     GDIFITATGNIKVINQWHLAKMKTHAIVANIGHFDAEIDLEYLKQYPWENVKPQVDQV
                     IFPDGKRIIVLAEGRLVNLGCASGHPSFVMSISFSNQVLAQMELWQHAEKYQKEVFCL
                     PKHLDEQVARLHLDKVGAVLTELTSEQADYIGVAKSGPFKPDYYRY"
     gene            complement(1602..>2107)
                     /locus_tag="VFP93_05390"
     CDS             complement(1602..>2107)
                     /locus_tag="VFP93_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HET9844071.1"
                     /translation="KNTIYHINPTGRFVLGGPQADSGLTGRKIIVDTYGGMARHGGGA
                     MSGKDASKVDRSGAYAARYVAKNIVAAGLADRCEIQISYAIGVAKPTSIYIETFGTGK
                     ISEEKLIQLIEQHFDLRPRGIIDMLSLLNPIYLPTATYGHFGRTEKNFTWEKTDKAEV
                     LKNDANL"
BASE COUNT          592 a          468 c          384 g          663 t
ORIGIN      
        1 attatcacca taaaataaag caattttttc gccgattttt aatcgtaata ctttcgcaca
       61 atgtaacgcg gcttgagggg ttaaaagaat ttcgtcgttg agcgagagcg gattagggtg
      121 atatatttta tgcaacatga gagcttagta ttttaaatgg tatatttcta gatgctttag
      181 catcgtttca aactagcagt gtaacccaag agatattctt tgagaagcac actataatgt
      241 tgactgacaa aacgatgcta actattattc tggatctaat aacgataata atccggttta
      301 aatggtccgc tctttgcaac cccaatataa tctgcttgtt ctgaggttag ttcggttaac
      361 acagctccta ccttatctaa gtgtagtctt gctacctgtt catctaaatg tttaggtaag
      421 caaaacacct ctttttggta tttttcggca tgttgccata attccatttg tgctagcacc
      481 tggttggaaa aagaaatgga cattacaaaa ctcggatgac ccgaagcgca accaagatta
      541 acaagtcgac cttcagccaa cacaataatc cgttttccat cggggaaaat aacctgatca
      601 acctggggtt taacattttc ccaaggatat tgtttaagat attcgagatc aatttctgca
      661 tcaaaatgcc caatatttgc cactatcgca tgcgtcttca tttttgctaa gtgccattga
      721 tttatcactt taatattgcc cgtggccgta ataaaaatat cgcctaaaga tgctgcataa
      781 tccatgctaa ccacgcgata cccttccatc gcagcttgta gggcacatat aggatcaatt
      841 tcggttatcc aaacggttgc tcctaaacca cggaaagctt gggcacaacc tttgcctaca
      901 tcgccaaaac ccaacacaac acaaattttc cctgcgatca tcacactggt tgcacgtttt
      961 atgccatcga tcaaggattc tctgcaacca tataaattgt caaattttga tttggtgaca
     1021 gagttgttta cattaatggc aggtactaaa agctttcctt cacgcatcct ttcataaaga
     1081 cgttgtacgc cggttgttgt ttcctctgat atacctttaa tttcttttaa gtagtgcggg
     1141 tatttttcat gcaacaattg ggttaaatcg ccgccgtcgt ctaaaatcat gttcgggcgc
     1201 caaccgtcgg gtccatccag cgtttgctca atacaccacc aaaattcttc ttcggtttca
     1261 cctttttttg caaaaaccgg tatattctta gcggccatag ctgcggcagc gtgatcttgg
     1321 gttgaaaaaa tattacacga agaccaacgt atttcagcac ccagcgcgat caatgtttcc
     1381 atcaaaaccg cggtttgtac tgtcatatgc aaacagcctg caattttagc cccctttaaa
     1441 ggcttcgtac tactatactc tttccttaaa gccataaggc ctggcatttc catttctgcg
     1501 atcgcaactt ctttacgacc ccaagcagct aaattaatat cctgtatcca atagtcttgt
     1561 gttgaagaag atttttttgc agtcatttgc atctgatttc cttacaagtt tgcatcattt
     1621 tttaaaactt ccgctttgtc tgttttttcc caggtaaagt ttttttctgt tcgtccaaaa
     1681 tgaccatagg ttgcggtagg caaataaatt ggatttaata acgaaagcat atcaataatg
     1741 ccgcgtggtc gcagatcaaa atgctgttct ataagttgaa tgagtttttc ttccgatatt
     1801 ttgccagttc caaacgtttc tatataaata gaagtaggtt tagcgacacc tattgcataa
     1861 gagatttgta tttcgcatcg gtctgcaaga cctgcggcga cgatattttt cgccacatac
     1921 ctggcggcat aagcgccaga gcgatcgact ttggatgcat cttttccaga cattgctcca
     1981 ccaccatggc gcgccatgcc accataagta tcgacaataa ttttgcgacc tgttaaacca
     2041 gaatctgctt gggggccccc taaaacaaaa cgcccagtag gattaatgtg ataaatagta
     2101 tttttat
//
LOCUS       DASTIB010000310         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1126163, whole genome shotgun sequence.
ACCESSION   DASTIB010000310 DASTIB010000000
VERSION     DASTIB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126163"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFP93_05395"
     CDS             <1..615
                     /locus_tag="VFP93_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012220704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET9844072.1"
                     /translation="RANSGLNNLRILLPMISGIHEVDESLRLVRQAYCEVVEEGALTE
                     FPPVGVMIEVPSSVYQAASIAKRVDFLSIGTNDLTQYLLAVDRNNNHVANLYDCLHPA
                     VLCALKMVVDACKKLGKPVSICGEMAGDPPSVIALLGLGFDMLSMNATNLPKIKWIIR
                     NFTFEKAKLILQECLMMENAIDVRLKLESVLEEAGLGGLVRAGK"
     gene            676..1467
                     /locus_tag="VFP93_05400"
     CDS             676..1467
                     /locus_tag="VFP93_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9844073.1"
                     /translation="MLDIFLFILIGLCAGLLAGMLGLGGGIIIVPGMVEWLTYKEMAT
                     DRIMQLSVGTSLSTVVIISLITGLMHRRLGYVVWDIVRFIAPGTTFGAVFGVLIADHL
                     SSDVLQRVFALLLCAIALQLLFFPHRPKSSQPILHSWQLTVCGIFIGALAGMLGIGGG
                     IILIPLLIYNGTPIIQASATSLWCVLPMVLVASCVSIWTGYNEVGLPTNSLGYVYWPA
                     TLIIGACALISSPIGIHFALKMSKIWLQRIFAVVLLIITIRMLVG"
     gene            1544..>2165
                     /gene="lgt"
                     /locus_tag="VFP93_05405"
     CDS             1544..>2165
                     /gene="lgt"
                     /locus_tag="VFP93_05405"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9844074.1"
                     /translation="MIQYPNIDPVALYITETLQIRWYGLMYIAGFVMAWFVMRNRTRN
                     LPKWKNVEVLNDFFFYAALGVILGGRIGYVLFYNFPGFLHDPLLLIKIWQGGMSFHGG
                     LIGVILAIWWFSYRHAIPFVEACDFCAPAVPLGLAAGRIGNFINGELWGKPTTVPWAF
                     QFPHVDSLGRHPSQLYGVLLEGALLFLILLWYSRKPRPMGNVAGVFL"
BASE COUNT          546 a          379 c          502 g          738 t
ORIGIN      
        1 cgtgcaaata gtgggctaaa taatttacgt attttactgc ctatgatttc tggtattcat
       61 gaggtagatg aaagtttgcg tttagttcgc caagcttatt gcgaagtggt tgaagaaggt
      121 gcattgaccg aatttccacc agtgggtgtc atgattgaag ttccatcgag tgtttatcag
      181 gctgcaagta tagccaaacg tgtcgatttt ttatcgattg gaacgaatga cttaacccaa
      241 tatttgttgg cagtggatag gaataataat catgttgcaa atctttatga ttgtttacat
      301 cctgcggtac tttgtgcttt aaaaatggtg gtggatgcgt gtaaaaaatt aggaaaaccc
      361 gttagcattt gtggggaaat ggcaggggat ccaccttctg ttattgcgct tcttggatta
      421 ggctttgata tgctaagcat gaatgcgacc aatttgccta aaatcaagtg gattattcgt
      481 aattttacct tcgaaaaagc taagcttatt ttacaagaat gtttaatgat ggaaaatgca
      541 attgacgtgc gcttaaaatt agaaagtgta ttagaagagg ccgggttagg tggcttagtt
      601 cgcgcaggaa aatagttctt aaaacatcgc atgtattctt gatactcgtc cttaattaca
      661 gcagaagtat ttaaaatgtt ggatatcttt ttatttattc tcataggctt gtgcgccgga
      721 ttattagcag gaatgcttgg tttaggcggc ggtattatta ttgtgcctgg aatggtggaa
      781 tggctaacct ataaagaaat ggccaccgat agaataatgc aattaagcgt tggcacttca
      841 ttatcgaccg tggtaattat ttctcttatt acgggattaa tgcatcgacg tttaggatac
      901 gttgtttggg atatagtgcg atttatagcg cctggtacta ctttcggggc agtatttggc
      961 gtcttaatag cggatcattt atcttcagat gttttacaac gcgtatttgc cttattacta
     1021 tgcgccatag cccttcagtt gttgtttttt ccacaccgac ccaaaagttc tcaacctatc
     1081 ttacacagtt ggcaattaac cgtatgtgga atttttatag gagcattggc aggcatgctg
     1141 ggaattggcg gaggcattat tcttattcca ctactcatct acaacggcac accgattatc
     1201 caagcatctg cgacctcttt atggtgcgta ttacctatgg tgttggtggc gagttgtgtt
     1261 tccatatgga ctggatataa tgaagttgga ttgccaacca atagcttggg ttatgtttat
     1321 tggccagcta ctttaattat aggggcatgc gcattaataa gttcacccat aggtatccat
     1381 tttgcgctga agatgtccaa aatttggttg cagcgtattt ttgctgttgt tttactaatc
     1441 ataacgattc gcatgcttgt tgggtaacca aaagcgcttt gagttgtcag gcatactcaa
     1501 tccccgatat tattaacctt taatgtattt tgctttcata tttatgattc aatatcccaa
     1561 tatcgatccc gtagcactct acataacgga aactctacaa atccgttggt atggactcat
     1621 gtacattgca ggatttgtga tggcgtggtt cgtcatgcgc aatcgcacgc gaaatctacc
     1681 gaaatggaaa aatgttgaag ttttaaatga ttttttcttt tatgcagctt taggggttat
     1741 cctagggggt cgcattggat atgttctatt ttataatttt ccaggatttt tgcatgatcc
     1801 gctcctttta ataaaaatct ggcagggggg tatgtctttt catggtggct taattggggt
     1861 aattttagcc atttggtggt tttcttatcg tcatgcaata ccgtttgtag aagcttgtga
     1921 tttttgcgca cctgcggtcc cgttagggtt ggcagcgggt aggattggta attttattaa
     1981 cggagaactc tggggcaaac ctactacggt gccctgggct tttcaattcc cccatgtgga
     2041 ttccttaggc cgacatcctt cgcaactata cggcgtacta ctcgaagggg ctttgttgtt
     2101 tttgattttg ctatggtata gccgaaaacc gcggcccatg ggtaatgtgg cgggtgtatt
     2161 tttag
//
LOCUS       DASTIB010000311         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_965133, whole genome shotgun sequence.
ACCESSION   DASTIB010000311 DASTIB010000000
VERSION     DASTIB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965133"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFP93_05410"
     CDS             <1..508
                     /locus_tag="VFP93_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844075.1"
                     /translation="SILLDNIYISRFDIRRLGNVPLFNIINMQMLCSVPKEDAHRIIQ
                     ELTEAVNNFNFNIDNPNYSYLFHRYKIKLCKISKLESEFKTKYNNFVTHAESAFQPGK
                     TGAILNHVATKVKPYYQSILDAKGGVVNVEAEIRTLLLNRLLVNHSVNEIAKIYIREE
                     YFNFGEWS"
     gene            687..1952
                     /locus_tag="VFP93_05415"
     CDS             687..1952
                     /locus_tag="VFP93_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844076.1"
                     /translation="MDENVEPEQKENRVGLFVSQLADIRRSNNRSLLETDKPACDTGH
                     FIDIAQMGSTHPILKLVELSLEDLFIRLVIPKFKARMEVLEPSEREALYESLVSVHVQ
                     NIPDILLDKVPFTEDLYRIRENFIKEIFGSTSNLVPLLEAEIDQSYPLEQGILKYELN
                     KDHNKALAKFYRLNICTNKKMSDALYDCYIQFKEEMIPQDTMLAMIKKLFEYGMQNED
                     LKPLLCNVIQHIASHELSDELAAYCQMQIHAVVMAAFNKTSIMYGMSDEFSIRCDQEK
                     LKTILSQWLNAPQFKTSIFDSVSSIVGNYEKMVHAIQQDFDMKVRRVNKMLPRFEKEK
                     EVAESFLNAIMDVNENLIVYEFAAVKKAILGFAQEFSIDSMTADTIHESETQERWKEF
                     SNNHPVLANRSNEILERYISSNVSNRIRI"
     gene            complement(2085..2573)
                     /locus_tag="VFP93_05420"
     CDS             complement(2085..2573)
                     /locus_tag="VFP93_05420"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET9844077.1"
                     /translation="MKNLKLKPIEPHIVSREPFTVVGLEYKGPMKNMVDIPKLWHLFN
                     DRIQEIENKMAEDRLYGVFYQTPSQRATSEMTYMACVPVSHDQEIPEGMISKEVQGGK
                     FAALTHKGPISDIVQSIDFLHSWILGSEFEFDQRESYELYDNRFQYESADSEFDIFLA
                     IK"
     gene            3010..>4203
                     /locus_tag="VFP93_05425"
     CDS             3010..>4203
                     /locus_tag="VFP93_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844078.1"
                     /translation="MYWMFWATPIQDISKWESLSETVSLEEKLQMYDIKLYSDYENAH
                     KACNPHIDKGVIKVSVPALQSEMLNQKEFEVPAYQIHYENSLFEHSDAKKIKSFLEKV
                     RVAKHQADIAEAKGELKELHDDIDALEENIEKLTQEIEVLNKKIDKKNNELAKKNTEL
                     TQKNAELAQKDEILEMKESEIKTAKEQNKSYAKASQDLNNAIFGYQTELEFKKRHLEE
                     LEKRAQEMEKRAQEIEKQVQIATNEKHDLESKLTTVEKNYKTITHEQQKHNDKLEQVI
                     ERLSYNLSETESELTREKEFHSRALQKTAALKWQLLNAANHFHVTMEKTLNNPETESE
                     PSTSVKPSFINNQETIVPWKHHQKTKQPAVKNFIAKNSFESSAALAGIALYCALDGIS
                     ATTGAL"
BASE COUNT         1527 a          699 c          726 g         1251 t
ORIGIN      
        1 cagtatactg ctggataata tttatatctc aagatttgat atacgaagat tagggaacgt
       61 accacttttt aatatcatta atatgcagat gttatgttcc gtaccgaaag aagatgcaca
      121 tcgtatcatt caggaattga ccgaagcggt aaataatttc aattttaata ttgataatcc
      181 caattatagt tatttattcc atcgttacaa aattaagtta tgtaaaattt caaaattaga
      241 atcggaattt aaaactaaat ataataactt tgtaacacac gcggagtcgg cttttcaacc
      301 aggaaaaaca ggagcaattt taaatcatgt tgctactaag gttaagcctt actatcagag
      361 tatacttgat gcgaaaggtg gggtagttaa tgtggaagcc gaaattagga cgcttttact
      421 aaaccgcttg cttgttaacc atagtgtaaa tgaaatcgca aaaatctata ttagagagga
      481 atattttaat tttggtgagt ggagctaacc aagacaaatt agacacttta cgattacatt
      541 taacatccaa cacagatatt gatcaactcg catggcgcat ctatgacccc tttgcaccgt
      601 cgattgggta tgaaaatctg ttaaccttaa acacgcccgc tgcaatccaa atacgtgaaa
      661 tgagtgcttt atatttctta ggtatcatgg atgagaatgt tgaacctgag caaaaagaga
      721 accgcgtcgg tttattcgta tcacaactag cagatattag aagaagtaac aataggtcgc
      781 tgttagagac agataagcct gcatgtgata ctggacattt tattgatatt gcccaaatgg
      841 gatccacgca tccgatatta aaattagtag aattgtcatt ggaagattta tttatacgtt
      901 tggtgatacc aaaattcaaa gccagaatgg aagttttgga accttcagaa cgtgaagcat
      961 tatatgagtc tttggtatcg gttcacgttc aaaacattcc agacatactt ttagataaag
     1021 ttccctttac agaagatttg tatcgaattc gtgaaaattt cataaaagaa atttttggaa
     1081 gtacgagcaa tcttgttcca cttttagaag ctgagataga tcaaagttac ccgctcgaac
     1141 agggtattct aaaatacgaa ctgaataaag atcataataa agcgcttgca aaattttatc
     1201 gtttaaatat ctgcacgaat aagaaaatgt ccgacgctct ctatgattgt tatattcagt
     1261 ttaaagaaga gatgattccg caagatacca tgcttgctat gataaaaaag ttatttgaat
     1321 atggcatgca aaatgaagat ttaaaaccac ttttgtgcaa tgttatacag catattgcct
     1381 ctcatgagct ctcagatgaa ttagccgctt actgtcaaat gcagattcat gctgttgtca
     1441 tggcagcttt taataaaact tctattatgt acggtatgag tgatgagttc agtattcgat
     1501 gcgatcaaga aaaattaaaa acaatcttat ctcaatggtt aaacgctccg caatttaaaa
     1561 catcaatttt tgatagtgtt agctcaattg tcggtaatta tgaaaaaatg gtacatgcaa
     1621 ttcagcaaga ctttgatatg aaggtgcgca gagtcaataa aatgttacct cgttttgaaa
     1681 aagaaaagga agttgctgag tcctttttaa atgcaattat ggatgtgaat gaaaatttaa
     1741 tagtttatga atttgcagct gtaaaaaagg ctattttggg atttgcacaa gagttttcga
     1801 tcgactcaat gacagctgat actattcatg aatctgaaac tcaagaaaga tggaaagaat
     1861 tctcaaataa tcatcctgtt cttgccaata gaagtaacga aatattagaa cgatacatta
     1921 gttcaaatgt ttcaaataga atcagaatat aattgcctgt agggataata ccttatccta
     1981 aggtgaagtg cattagtcag gtactttcaa gatatacaca aaacaattat gtgtaccaat
     2041 gactgtgcac tataattggc aaaatcttat aacccatttt aaaattattt aatagctaaa
     2101 aatatatcaa attctgaatc tgcagattca tattgaaaac gattatcata cagttcatag
     2161 gattctcttt ggtcgaattc gaattctgat cctaaaatcc aactatgcag aaaatctatg
     2221 ctttgcacga tatcggaaat tggaccttta tgcgttagtg ccgcaaattt accaccctgg
     2281 acttctttac ttatcatacc ttctggaatt tcctgatcat gggaaaccgg cacacaagcc
     2341 atataggtca tttcgctggt tgcacgttgc gatggcgttt gataaaaaac accatacaag
     2401 cgatcttcag ccattttatt ttctatttct tgaattcggt cattaaaaag atgccaaagc
     2461 ttcggtatat ctaccatgtt tttcataggg cctttgtatt ctaagcccac taccgtaaag
     2521 ggttcacgac taacaatgtg tggttcaatt ggttttaatt ttaaattttt catggggttc
     2581 tccctatttt gtgaaacctt tgcattcata gttgggttta gatctagtta tcttgcaaaa
     2641 aaactttatg cttttaaccc attataggtg aaactcttta tctatcattt ggttgttagt
     2701 taagacatta aataattaac ctcattttgt gtggaaaaat caaaaaaata ctttatttag
     2761 attctattta ttttgcttta acgcaaaaaa catatcgata ggttgtacga attctaactt
     2821 gtcttaatta tacatgtcat tttaccattt tctttaatat atccatgaaa taccgccaaa
     2881 tttgtgccgc ttttcaaata taccaagatt ttttcgacaa atttctatgt tccttgactg
     2941 aaaattaaac ttaggataaa aacccacact gcaattgatt ttcgcatgaa gtttaaataa
     3001 ggagattcta tgtattggat gttttgggct acccctattc aagatataag caaatgggaa
     3061 agcttatcag aaactgtttc tctcgaagaa aaattgcaaa tgtatgatat aaaactatat
     3121 tctgattacg agaacgcaca caaagcttgc aatcctcaca tagacaaagg cgtcatcaag
     3181 gtatctgttc cagcactgca atcagaaatg ttaaaccaaa aagaatttga agttcctgca
     3241 tatcaaatac attatgaaaa ctcgttattt gagcactcag acgctaaaaa aataaaatcg
     3301 ttcctagaaa aagtccgcgt tgccaaacat caagcagata tagcggaagc aaagggagag
     3361 cttaaagaac tgcatgatga tatagatgcc ttagaagaaa atattgaaaa attaacacag
     3421 gaaattgaag tattaaataa aaaaattgat aaaaaaaata atgagttagc aaaaaaaaac
     3481 acagagttaa cgcaaaaaaa tgctgaatta gcgcaaaaag acgaaatcct ggaaatgaaa
     3541 gaaagcgaaa taaaaaccgc taaagaacaa aacaagtcct atgctaaagc ttcacaagat
     3601 ttaaacaatg ccatttttgg ctatcaaaca gaattagaat ttaaaaagcg gcatttagaa
     3661 gagctggaaa agcgggcgca agaaatggaa aagcgggcgc aagagataga aaagcaggtg
     3721 caaattgcca caaatgaaaa acacgattta gaaagtaaat tgacaaccgt tgaaaaaaat
     3781 tataaaacaa taacgcatga acaacaaaaa cataacgaca aacttgaaca ggttattgag
     3841 agattatctt ataatttgtc agaaacagaa agcgagttga cacgcgaaaa agaattccat
     3901 agccgagcat tacaaaaaac agcagcatta aaatggcaat tgcttaatgc agcaaatcat
     3961 tttcatgtaa ccatggaaaa aacacttaat aacccagaaa ccgaatctga gccttctact
     4021 tcagtgaaac cttcttttat aaataaccaa gaaacaatcg taccatggaa acaccatcaa
     4081 aaaacgaagc agcctgcagt taaaaatttt attgctaaaa attcattcga atcaagtgca
     4141 gcgcttgctg gcattgcttt atattgcgca ctcgatggga ttagcgctac cactggcgca
     4201 tta
//
LOCUS       DASTIB010000312         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_768360, whole genome shotgun sequence.
ACCESSION   DASTIB010000312 DASTIB010000000
VERSION     DASTIB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768360"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFP93_05430"
     CDS             complement(<1..707)
                     /locus_tag="VFP93_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9844079.1"
                     /translation="MIFNDEIQLIRETARNFAHEELKPNSSRWDKNAHFPEETLNAMA
                     KLGFLGMLIPEMYGGSNVGYLAYCLVIEEIARGDGATSTIVSVHNSVGCLPIYKFGTN
                     KQKEIYLPKMCRGEWLGAFCLTEPQAGSDAKNIKTRAQVYKDAYVLNGVKQFVTNGKH
                     ASIAIVFAKTADVEKNSISAFIVPTTTPGFTITRVEHKMGQKASEIAQITLENCKLPK
                     ENLLGEQGQGYKIALSN"
     gene            855..1931
                     /gene="pdhA"
                     /locus_tag="VFP93_05435"
     CDS             855..1931
                     /gene="pdhA"
                     /locus_tag="VFP93_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET9844080.1"
                     /translation="MSQTTSVAQFEVKYYRYLDPAGKVEQTLPDFAQNTTHLQKLYKM
                     MLKTRVFDTKAITLQRTGKLGTYPSTLGQEAIAVGIGAAMHADDVLCPYYREYGAQFW
                     RGVTMENILLYWGGDEQGSNFEGFAKKDFPICVPIASQTLHGVGVASALQYKKTKNAV
                     VTTVGDGGTSRGDFYEAMNVAGIWNLPIVFVINNNQWAISVPRSQQTKAQTLAQKGIA
                     AGLSCVQIDGNDVIAVQDSLQTALTQARSGKGATVIEALTYRMCDHTTADDASRYRPP
                     QDLEANKKIDPILRLKNYMMEQNSWNEDDEKSYMEQTVKEVNDAVERYLAVPINKPHT
                     MFDYLYAELPRAFQEQYDELKARV"
     gene            1935..2912
                     /locus_tag="VFP93_05440"
     CDS             1935..2912
                     /locus_tag="VFP93_05440"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020286267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9844081.1"
                     /translation="MPVITLIEAVTQALAYELEHDEDVLLFGEDIGVNGGVFRATVGL
                     QEKFGQNRVRDTPLAENLIAGLAAGMSASGLKPIAEIQFMGFIYSTVDQVVSHISRLR
                     NRTRGRLSCPLVIRAPFGGGIHAPEHHSESTEAIFAHIPGLRVVIASSPARAYGLLLA
                     SIRDPDPVIFLEPKRIYRKVKEDVPDNGEALPLDVCFKIREGNDITLITWGAMIHETL
                     EAAAKLEQKGIEAEVIDVATIKPLDIETILESIAKTGRCVIVHEAARTCGVGAEIAAQ
                     IAEKALTSLLAPIKRVTGYDTIMPLYRLEYEYLPSVQRILTACSEVLEF"
     gene            2925..>3753
                     /locus_tag="VFP93_05445"
     CDS             2925..>3753
                     /locus_tag="VFP93_05445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9844082.1"
                     /translation="MNIFKLPDLGEGLPDAEIVTWHVKEGDTVTQDQLLVSMETAKAV
                     VDVPSPYSGKVTKLHGNPGDIIETGKPLVSFEGEKNAKTDSGTVVGKVEVGSEVVQEK
                     ASSISRSSNVKVLPAIRALAKKLNVDLTTITPSGPQGTITKEDVEQAHERLVQAGPLE
                     LLRGVRRSMAITMQQSHSEVVPVTIFEDADITDWKNKDFTVRLLKAIAHACLKEPALN
                     AWYDGQAMGRRLLNYCNIGLAMDTQEGLFVPVIENVQDKNISELRDEIETLKEEVLNR
                     "
BASE COUNT         1160 a          721 c          819 g         1053 t
ORIGIN      
        1 aaattgctta aagcaatttt atacccttgt ccttgctcgc ctaataaatt ttccttagga
       61 agtttacaat tttctaaggt gatttgtgca atctcggaag ctttttgccc cattttgtgt
      121 tcaacacggg ttatggtaaa acccggtgta gtggtaggaa caataaatgc acttatagaa
      181 tttttttcga catcggcagt ttttgcaaac acaatagcaa tactagcgtg tttgccattt
      241 gtcacaaatt gtttcacgcc attaagtaca taagcgtcct tatatacttg tgctcgcgtt
      301 tttatatttt ttgcatcgga cccagcttgt ggttctgtta aacaaaaagc acctaaccac
      361 tcaccacgac acattttagg caaataaatt tctttttgtt tgttcgtacc gaatttataa
      421 ataggtaagc agccaacact attgtgtaca ctaacaatag ttgaagtggc accatcgcct
      481 cgagcaattt cttcaataac taagcaataa gcaagatacc ccacattgga accaccgtac
      541 atttccggga ttagcatgcc taaaaagccc agctttgcca ttgcatttaa cgtttcttct
      601 ggaaagtgag cgtttttatc ccaacgagac gaattgggtt ttagttcttc atgcgcaaag
      661 tttctggcag tttcacgaat cagttggatt tcatcgttaa aaatcatacg cttcttataa
      721 aattttaatt aaagctacca cgctaacgca taaaaaccta taggttaggt tagcctcagg
      781 cttacaggct tgataaaatg cagctattag tgtgcagccc agcattttgc ggtgcaattt
      841 aaaaggagag cttagtgtca caaactacat cggttgcgca atttgaggtt aagtattatc
      901 gctatttaga tcctgcaggt aaagttgagc aaactttgcc tgattttgcg caaaatacta
      961 cacatttaca aaaactttac aaaatgatgt taaagacacg tgtttttgac accaaagcta
     1021 ttacattaca acgaaccggc aaattaggaa cctatccttc cacactagga caagaagcca
     1081 tagcggtagg aattggcgct gccatgcatg ctgatgatgt actgtgtccg tattatcgtg
     1141 aatatggagc acagttttgg cgtggcgtca ccatggagaa tattttgctt tattggggtg
     1201 gcgacgaaca aggtagtaat tttgaaggat ttgcaaaaaa agattttccc atttgcgtgc
     1261 ccatagcaag tcaaaccttg catggtgtag gtgtcgcaag tgcgcttcaa tacaaaaaaa
     1321 ccaagaatgc cgtcgtaaca accgtgggcg atgggggcac atcgcgtggt gatttttacg
     1381 aagcaatgaa tgtagcaggt atttggaatt tacctattgt cttcgttatt aataacaatc
     1441 aatgggcaat ctctgtgcca cgtagccaac aaacaaaagc acaaacatta gcgcaaaaag
     1501 ggatcgcagc gggattatct tgtgtacaaa tcgatggcaa tgatgtaatt gcagttcaag
     1561 attcccttca aacagcatta acacaagcgc gtagtggcaa aggtgcaact gttattgaag
     1621 cactaacgta tcgtatgtgt gatcatacca cggcggatga tgcaagccgt tatagaccgc
     1681 cccaagactt agaagcaaac aaaaaaattg atcctatctt gcgactaaaa aactatatga
     1741 tggagcaaaa tagttggaat gaagatgatg aaaagtctta catggaacaa acggtaaaag
     1801 aagtgaatga tgcggtagag cgttatttgg cggtacctat aaacaaacca cataccatgt
     1861 tcgattattt atacgcagaa ttacctaggg cttttcaaga acaatacgat gaactcaaag
     1921 cgagggttta aattatgccg gtgataacac tcatagaagc agtaacccaa gctttagctt
     1981 atgaattaga acatgatgaa gatgtattac tatttgggga agatatcggc gtaaatggtg
     2041 gtgtctttcg tgcaaccgta ggacttcaag aaaaatttgg acaaaatcga gtgcgcgata
     2101 cacccctggc tgagaatcta attgcaggat tagcagccgg gatgtctgca tccgggttaa
     2161 agccaattgc tgagatacaa tttatgggat ttatttattc cacagtcgat caagtcgtta
     2221 gccacatttc gcggttacgt aatcgaacac gcggaagact cagttgtcct ttggtgattc
     2281 gtgcgccttt tggtggtggc attcacgctc cggaacatca ctctgaaagt accgaagcta
     2341 tctttgccca tattcctggt ttacgggtag ttattgcgtc ctcacctgca cgtgcctatg
     2401 gattattatt ggcaagtatt cgtgatccgg atcctgtgat ttttttagag cctaaacgca
     2461 tctatcgaaa ggtaaaggaa gatgtgccag ataatgggga agcgttaccg ctagatgtgt
     2521 gttttaaaat tcgcgaaggc aatgatataa ccttgattac ttggggtgct atgattcatg
     2581 aaaccttgga ggcggctgct aaattagagc aaaaaggaat tgaggcggaa gtaattgacg
     2641 ttgccactat caaaccttta gacatcgaaa cgattcttga atcgattgcc aaaactggca
     2701 gatgtgtcat tgttcatgaa gccgcacgca cctgcggggt tggagcggaa atagcggcac
     2761 aaattgctga aaaggcgctt acatccttgt tagcacccat taaacgcgtt accggttacg
     2821 atacgattat gcctctctat cgtttagaat atgaatattt gccaagtgtg caaagaattt
     2881 taactgcctg ctccgaagtt cttgaatttt aataagggaa cctaatgaac atttttaaat
     2941 taccagatct cggggaaggt cttcccgatg ccgaaattgt aacttggcat gttaaagaag
     3001 gggatacagt cacacaagat caattattgg tatctatgga aactgcgaag gcagtagtcg
     3061 atgtaccctc cccttattct ggtaaagtaa ccaagttaca tggtaatccg ggcgatatta
     3121 tagaaacggg gaagccactc gtaagctttg aaggagagaa aaatgcaaaa actgattctg
     3181 gcacagtagt aggtaaagtc gaggttggct cagaagttgt gcaagaaaaa gcttcttcta
     3241 tatctcgaag cagcaatgtc aaagtattgc cagcaattcg tgctttggca aaaaaactca
     3301 atgtagattt gacaaccatt acaccaagcg gaccgcaagg aacaataacc aaagaagacg
     3361 tagagcaagc acatgaacga ctggttcaag cgggcccttt agaactatta cgtggggtgc
     3421 gacgctccat ggcaattact atgcagcaat cccatagtga agttgtgcct gtaactattt
     3481 ttgaagatgc tgatattact gattggaaaa ataaagattt cacggttcgt ctattaaaag
     3541 caattgccca tgcctgttta aaagagcctg cactaaatgc ttggtacgat ggtcaggcta
     3601 tggggcggcg tttgttaaat tattgtaaca tcggcttagc catggatacc caagaaggtc
     3661 tttttgttcc tgttatagaa aatgtacaag ataaaaatat ctcggaatta agagacgaaa
     3721 ttgaaacatt aaaagaagaa gtattaaatc gct
//
LOCUS       DASTIB010000313         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1051966, whole genome shotgun sequence.
ACCESSION   DASTIB010000313 DASTIB010000000
VERSION     DASTIB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051966"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..622)
                     /locus_tag="VFP93_05450"
     CDS             complement(383..622)
                     /locus_tag="VFP93_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844083.1"
                     /translation="MGSCLHKGNDSLWVAYSRWPDKATRDAAWPGDNASNKELPKDIR
                     DTIQKMQALKEENHDLENYDEICLEVVEDLLLRHI"
     gene            complement(739..2025)
                     /locus_tag="VFP93_05455"
     CDS             complement(739..2025)
                     /locus_tag="VFP93_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9844084.1"
                     /translation="MSFENLGLSAPLVQALHEKGYREPSPIQLQSIKAILKKIDLMAC
                     AQTGTGKTAGFVLPILELLSMKIQKNTQPRALIIAPTRELAIQIHKSIMEYGKNLTLT
                     STSIYGGVKVGRQIKRLSDGVDILVATPGRLLDLYQQRAVQLNNVEMLVLDEADRMLD
                     MGFVHDIKKIISRLPKKRQTLLFSATFSDDINKFAKGLLSNYEEIKIAPKVTSAASVQ
                     HKIHPVDKSKKFSLLIHLIQKNNWYQALTFIRTKHSANKLVKLLNQNNIRALAIHGNI
                     SQLQRVNALESFKQGKIQILVATDIASRGIDIAGLSQVINFDLPNVPEDYVHRIGRTG
                     RAGSSGQAISLVSAEEIKQLNDIEKILKSPIKRETIEGFEPVHALPSRVKQGTSSKRK
                     PERTEKNSFDNSFVRKKKGTFGLNGTKKNKKIKFAN"
BASE COUNT          800 a          464 c          479 g          832 t
ORIGIN      
        1 ttgtaaattg ccggctgaac ggcatcaggt cagtaatcta aatctacttg tcattgatgg
       61 caaataagct cgaaaagccc tcaaggtgta catctatttt aaaagaaaat ccagtaggat
      121 attttatgat ttgtgcaaaa accattaacg cacgttcatg aaggtggtaa taaagctact
      181 cttttagctg caagaaagaa cccatggaca attgttcagg agttattaaa aataaaaaag
      241 tgcaagaaac ataaatgcaa gagataagaa gtcctatact ggactttgct ctatcaaatt
      301 atctaaaaca agaaaattta atgatgcccg atctgcagga agagtattca aacttcgaag
      361 gatttttaag tctatactcg actcaaatgt gtctcaataa aagatcttcg actacctcta
      421 aacagatttc atcataattt tctaagtcat ggttctcttc ttttaacgct tgcatttttt
      481 gaatagtgtc gcgaatatcc tttggtagtt ctttattaga tgcattgtca cctggccatg
      541 cggcatcgcg agttgctttg tctggccatc gagaataagc aacccacaga ctatcattcc
      601 ctttgtgtaa gcaagaaccc atagcaccac aattttgtgc aaagtaagtg gcaattttat
      661 gccaatattg atccttgtca aactaagctt ttatgcaatt tttgaattct agtttatttc
      721 ctaaaaatag aaaaaagttc aatttgcaaa tttgatcttt ttgttctttt ttgtgccatt
      781 cagaccgaag gtgcctttct tttttcggac gaacgaatta tcgaaagaat ttttttcggt
      841 ccgctcgggt tttctttttg aagacgtgcc ttgtttaact ctactaggta atgcatggac
      901 aggttcaaaa ccttcgatgg tttctcgctt aattggactt ttgagaattt tttcaatgtc
      961 atttagttgc ttgatttctt cggcacttac caaggaaatt gcttgtccgg aagaacctgc
     1021 acgccctgtc cgcccaattc ggtgtacata atcttcggga acattgggta aatcaaaatt
     1081 aataacttgg ctcaggccag caatatcgat tcctcgcgat gcgatatcgg tagcgactaa
     1141 gatttgaatc ttaccttgtt taaaactttc taaagcgttc actcgttgca attgacttat
     1201 attcccgtgg attgccaagg ctctaatatt attttggttt aacaatttaa ccagtttatt
     1261 agcgctgtgt ttagtacgaa tgaatgttaa tgcttggtac caattatttt tttggattag
     1321 atggataagc aacgagaatt ttttactttt atctacagga tgaattttat gctgaacgct
     1381 tgcggcagag gtaactttag gtgctatttt aatttcttcg taattactca ataatccttt
     1441 agcaaatttg ttaatatcat ccgaaaaagt agcggaaaat aaaagggttt gccttttttt
     1501 aggtaaacgt gaaatgattt tttttatatc atgaacaaat cccatatcca gcattctatc
     1561 ggcttcgtcc aacactagca tttctacgtt atttaattga acagcacgtt gttgatatag
     1621 gtctagtaat ctccccggag ttgctaccaa aatgtctacc ccatctgata acctcttaat
     1681 ttgtcgccca actttgaccc ctccataaat gctcgtcgaa gttagggtta gatttttacc
     1741 atattccata atagatttat gaatctgaat cgccaactcg cgagttgggg caattataag
     1801 ggctcgaggc tgtgtatttt tttgaatttt cattgaaagt agctctaaaa taggcaaaac
     1861 aaacccggct gtttttcccg tacccgtttg cgcgcaagcc attaaatcaa tttttttcaa
     1921 aatcgccttg atagattgga gttgaatagg agaaggttca cgataccctt tctcatgaag
     1981 agcttgaacc aaaggcgctg ataaaccaag attttcaaaa gacataataa tacctaaaaa
     2041 tgatgttaaa catcgaaaaa tgaatgatgc tgttaaatca actgtttatt gagtgcacac
     2101 aatggcacct tgcgacataa tgaaattgga tgttagataa gagtggcaat tcaattaaaa
     2161 tgacgcgctt gctattaaaa ttttaataag tatgaaatta tacctgaatt gcggattaaa
     2221 tctagttatt aagtcgtatt attaaccttt gattatcata aaatttcgta gaaaaatcgg
     2281 atgtactggg tcagcatttc tgggagaaag agccaggaaa ttagcgcgtc cagtagtttt
     2341 gaaaccgaaa gtgtctaggc aatgggaacg ctataaaagc gcttgggacg tcttaaagct
     2401 cttactaaaa tgacgagaag ctcaagctga acgtaggatt taataagtag agttagccaa
     2461 atgtctcaca aaaatgctgc caacgaattc ggtattgaag aagcactagt gagaaagtta
     2521 tgaaatgcac aattccctca agggcaaaac tttatttcga aggtcaaacc cgatg
//
LOCUS       DASTIB010000314         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1245842, whole genome shotgun sequence.
ACCESSION   DASTIB010000314 DASTIB010000000
VERSION     DASTIB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245842"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFP93_05460"
     CDS             <1..526
                     /locus_tag="VFP93_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844085.1"
                     /translation="ITNGSLIIEGDVHDRSYIEITTNTKNVSFWQKVKNFYFQPPITS
                     NLNVEGNVGNNVQFVIEGSILIGGDIKNYNIINAQNGDIHVGNVGIGTQLTSTFGSVH
                     CMKVNREVKLTTFFGKANTSDNKKYQNSIFDLNYFSQLFFLQSAKSKPPMRDVRNSIT
                     TILDPNIKRSTVGK"
     gene            complement(533..939)
                     /locus_tag="VFP93_05465"
                     /pseudo
     CDS             complement(533..939)
                     /locus_tag="VFP93_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(864..1055)
                     /locus_tag="VFP93_05470"
                     /pseudo
     CDS             complement(864..1055)
                     /locus_tag="VFP93_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            1932..2834
                     /locus_tag="VFP93_05475"
     CDS             1932..2834
                     /locus_tag="VFP93_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9844086.1"
                     /translation="MQKIIPHLWFDQEAVEAANFYVSIFENGSNLINTETIHDTPSGD
                     CDIVTFDLQDYRFMAISAGPLFKFNPSVSFLLNFDPAKDKNAREKLDAYWERLSSQGS
                     VLMELDTYPFSERYGWLQDKYGVSWQLILSDKQGEARPFITPFLMFIGSNCGKAEDAI
                     NYYCSLFKNSKRGRTAYYPSGSAPDKEGSVMFADFQIEDQWFAAIDSAHKHPFAFNEA
                     ISFIVNCKNQKEIDYYWEKLSAVQESEQCGWLKDKYGLSWQIVPDILQELMSGDEQKK
                     AKVTQAFLKMKKFDIAELERAAKT"
BASE COUNT          922 a          482 c          523 g          961 t
ORIGIN      
        1 aatcacaaat ggttctttaa ttatagaagg ggatgtacat gatcgtagtt atattgaaat
       61 aactacaaat accaaaaatg taagtttttg gcagaaggtt aaaaattttt actttcaacc
      121 ccccattacg tccaatctaa atgtggaagg taacgtaggt aataacgttc aatttgtgat
      181 cgaaggcagc attttgattg ggggtgatat caaaaactat aatattatca atgctcaaaa
      241 tggcgatatt catgtcggaa atgtaggtat tggcacgcag ttaacatcga catttggttc
      301 tgtacattgc atgaaggtta acagggaagt aaaacttaca actttttttg gcaaagcaaa
      361 cacttccgat aataagaagt accaaaattc aatatttgat ttaaactatt ttagtcaatt
      421 atttttttta cagtccgcaa aatctaaacc acccatgcgc gatgtgagga attctataac
      481 aacaatttta gatccaaaca taaaacgatc gactgtaggc aaataatcca ttttaattat
      541 aagctgctct ttttgatagt gaattatttt ctagaagatt ttcataggtg attcctaatt
      601 caatatgaat ttttatagcc aaggaaggtg aaatggatct ttttccattc aatatttctg
      661 aagctctatt agatccaacg atatgggcaa aatcagactg cgtcatgcct agttgttcca
      721 atcggaattt gatagctgaa attggatcag gtgcaacaat cggaaatttt tcttcttcgt
      781 acttttcaat aagaattgca agtattctaa ttcatctcct tccggagttc caagtttagc
      841 atccattaag tgttcgacgc gtttcaaagc agtttgataa tctttttcag ttctaatcgg
      901 tttgattttc ctatagtcct cgcatcgatt ttctcgcatt gcctatgggt accaataaat
      961 cttatatata ttatttgtct gggataattt atctgaacaa ttaatctata tttatttcca
     1021 tggatattaa atacaacagg gttatctgct aaaaactcgc atttctatat aatgcattta
     1081 tcgtattggg atttccaaag ataaaattta ttgcatagca gctgattaaa gaaaaaaaca
     1141 cgaagattct tgccaatcaa aaaaacttta tatagtctat gcaggtaatt cacgcagttt
     1201 gtggatcgcg ccctcaaaag tggtgacaca ctttcgagag cacttaccat ttcactaaat
     1261 cttaatcgga gattcagaaa aaatgatcaa ctctatttta acgtaaagcg ttccaaataa
     1321 aagtcaattc cgataaccaa aacctatttt ggcgctaaat ctttgttcca aaaaaagttt
     1381 tcgtccaaaa ttctttttat gttggttgtc tgccaatccc ttgccgcaac ggtaagggtt
     1441 cttgaatgct tagtaggatc gatatatgag cgaacggata ctttggctat tgtttacggc
     1501 taatacattt gtgggagtgc gatttttaag taatttcttt ttaaaccgag aatggagtct
     1561 tctaatacca tttttattgt gtatttttat ttataatttt catggtttta ttttttatca
     1621 cacgattgcg gatgctttat cgctcgcgtt tacgctaacg ttatatacga cgtttatagg
     1681 cattttaatc tcgaacttat attccgatgg gtatatggag ttttaaatga aatttaaaaa
     1741 gagtgattgg agttgaaaag tggacttgca ttgcatcaat tataatttta atactattta
     1801 atattaagtc agttacaaca acaaccttct gaaggtgtgc atttcgttat ctatactatc
     1861 tgaaatagga taataactgc aggtatttaa aacttcctgt atcgatttac ccaacctttc
     1921 aagagaataa catgcaaaaa ataattccgc atttgtggtt tgatcaagaa gctgttgaag
     1981 cagctaattt ttatgtatct atattcgaaa atgggtccaa tcttataaat acggaaacta
     2041 ttcacgatac tccctcaggt gattgtgata ttgtaacttt tgaccttcaa gattatcgtt
     2101 ttatggcaat cagtgcaggg ccgttattca aatttaatcc atcggtatcg tttctgctca
     2161 attttgatcc tgctaaggat aaaaatgcac gagaaaaact agatgcctat tgggaacgct
     2221 tatcctcgca aggcagtgtg cttatggagc ttgatacata cccttttagt gaacgctatg
     2281 gttggttgca agacaaatat ggcgtctcct ggcagttgat attatcggat aaacaaggcg
     2341 aagcaagacc ttttatcacg ccgtttctta tgtttattgg cagtaactgt ggtaaagcgg
     2401 aagatgctat caattactat tgctcgctat ttaagaactc gaaacgagga aggactgctt
     2461 attacccaag tggaagtgct cccgataaag agggtagtgt tatgtttgca gattttcaaa
     2521 ttgaagatca atggtttgca gcgatcgatt ccgcgcacaa gcatccattt gcttttaatg
     2581 aagctatttc gtttatagta aattgtaaaa accaaaaaga aattgactac tattgggaaa
     2641 aactttccgc agttcaagag tccgagcagt gtggttggct aaaagacaaa tatggtttgt
     2701 cctggcagat cgtacctgat attttacaag aacttatgag cggtgatgag cagaaaaaag
     2761 ctaaagtcac gcaagcattt ctcaaaatga agaaatttga tattgctgag cttgaacgcg
     2821 ccgcaaaaac gtaatatttc taagcatgga taccgaaaaa atatgcgcaa acttatagtt
     2881 ctaacgtt
//
LOCUS       DASTIB010000315         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_601785, whole genome shotgun sequence.
ACCESSION   DASTIB010000315 DASTIB010000000
VERSION     DASTIB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601785"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFP93_05480"
     CDS             complement(<1..232)
                     /locus_tag="VFP93_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014998301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9844087.1"
                     /translation="MRLSKIKLAGFKSFVDPTTIHLLGNLSAIVGPNGCGKSNVIDAV
                     RWVMGESSAKNLRGGQLVDVIFNGSTSRKPSGQ"
     gene            300..707
                     /locus_tag="VFP93_05485"
     CDS             300..707
                     /locus_tag="VFP93_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9844088.1"
                     /translation="MNKDVPSQEKLTILLVENSRTARAVMTALLSNSGYTVHPAGTGP
                     EALQVLDSQSVDVVIMDVFMPLMNGYEVTKAIRSSNKPYANIPIIAYTASKNPRDEVQ
                     CKEAGMNEYLVKTEDNKPLLSWLKNYSSGSTEQ"
     gene            799..2634
                     /locus_tag="VFP93_05490"
     CDS             799..2634
                     /locus_tag="VFP93_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9844089.1"
                     /translation="MLAVFQQLQDSAQLLKPYFTDHKNASKNKWLFFGFLVGNILQAV
                     VIAVLYTYMSLFIGIFAMPVLSYALLFSTLAECSFALLAYTATSVVNNYLTTSLKYSL
                     SESLNADLSAQWLRNQNFYGIKQPGNNTVYPDKVLSEDSITLVKKSIDLLQLSFNVAF
                     GFIIGVYRLFALSVVTTITVFNREIAIPGLMAIGSIGYALVYNLISARVSNSLTAQVN
                     VEKEKSGHMQKVMDHIDANHGALAMQNAAHREQGFYNHAFNDLKDAKLKVLNTTTKLD
                     FLSIMNESLSVLVGLILAAPSIIAKKIDATSMLEINHYVSDIIKFFTVKYDNIHDITQ
                     LETSVQRINEFKKAMATWEKSYQKNQHFTSPESSFSVSNLTIKNPRNETLLDKVNVTF
                     EKGKITLLKGESGCGKSTLLRTLAGLWPKEGTYSLPFKREEIAIIPQTPCFPFRSKLI
                     EAIAENPESINRDQLKAYMNELELSHLIPYLDKEGSWSTVSGGERQRLAILGILTKTS
                     QPKLVFLDESTSALDIKLKAKTEDLIKRVLKNTTILAVDHNPEHTAKFHKAPFYDTTV
                     EFRNKKLHTAHAPSNTPKPVIFSEKHPKASLPKQPLSANLHPSYH"
     gene            complement(2660..4189)
                     /locus_tag="VFP93_05495"
     CDS             complement(2660..4189)
                     /locus_tag="VFP93_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9844090.1"
                     /translation="MKGAQVKPPTTNASQAETSVVKIFTTALKHDYKFPWRAPKIVRW
                     TGSGFLVQSDNGIKLITNAHVAAGATHLEIQLAHDSKKYCATLQDIAHECDLAILEVE
                     NKDFWRKIQALQLGSVPKHEEKVKVIGFPLGGKTVSITKGIVSRIEQDRYAHSGREFI
                     TTQISAPINPGNSGGPVLNSNGDVVGVVHQGYPGAQNLGYMIPVNILTNFIKQSSQQK
                     KGFPDLGISIQRLENFYLRKHFKLLSHQSGVLIKSIANLSSCKGILRKEDIILAIDDV
                     PLNFDGSICMHPMKKMDFHYLIQNKSIGDSVRFRILRQGKMLDLNVKLEQSIGATESI
                     LTEHDNPPTYYIISAQLVVQPVSQNFIQAKRLYFANRDKEKPQDHLVAIRTILSSCYT
                     VGYKAEGELIAEVNGIKIRNIHDVIKACETNGKDTHMLKTENGTILVIPNLTQAKQQE
                     ILHQYNITQNRSENLRNQSIEPVSYNHKPTVLKFSKALQKPITKLHAENIYLTKKSNC
                     S"
     gene            complement(4362..>5590)
                     /locus_tag="VFP93_05500"
     CDS             complement(4362..>5590)
                     /locus_tag="VFP93_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844091.1"
                     /translation="KERISTFLKTYCSNFSFKEHLTIEENISENIRALKVKRAKNHKT
                     LTELSDEIAHLESQQNTWQGLCRQYPQLSTTTQWIDAWSQLLTIFGSKLLHGVKLLSS
                     RQGSKTYFDITFYNTADLKMAHTIDKKFDLLIVTHAHDFSLPLSFKLLRQAQKALIFG
                     HPKLTLSYPIVLSDIDSLALKTLMGIATEEVLEDLQYKGKVLSQTSLYYLAEQQSYYK
                     QYDPTGLYYDRPLAFSEIWCQPNLAAFYKTYIDKKCIVHPLLGAGLKFFHIIGEVLDQ
                     EGWFNPKEADCLITWLKENSADNLAIITLSNAQKIYLEEQCRMLACSVPVYLLKNCPE
                     HRYKTILFSPVYTLYSPRPFLLDCGDAMLYRVLSRAKESLIIFADTRIFDEKTHSPTG
                     NLAKIMPQLDAKSMTM"
BASE COUNT         1710 a         1072 c         1007 g         1801 t
ORIGIN      
        1 cttgaccgga tggtttgcgg gatgtagatc cgttaaaaat cacatccact aattgaccac
       61 cacgtaaatt tttagccgag gactctccca tgacccagcg cactgcatcg ataacattag
      121 attttccaca accgttgggt ccaactattg cagataagtt gcctaacaaa tgtattgtgg
      181 tcgggtctac gaatgattta aaccctgcta attttatttt ggagaggcgc ataaagccaa
      241 agctcgatct aaactaataa gtagtgtatg ttagcgacag attataccat ggatactcta
      301 tgaataagga tgttccctct caggaaaagt tgactatatt actagtggaa aacagccgaa
      361 cagctcgtgc cgttatgact gcgttacttt ctaactctgg ctataccgtc catcctgctg
      421 gcaccggtcc tgaggctcta caagttttag attcacaatc cgttgatgta gtcatcatgg
      481 atgtttttat gcctttgatg aatggctacg aagttaccaa agccatacgc agttcaaata
      541 aaccctacgc aaatataccg attattgcgt atacagcaag taaaaatccc cgcgatgaag
      601 ttcagtgtaa agaagctggt atgaacgaat acttagtaaa aactgaagat aacaagccac
      661 tactaagttg gctaaagaac tactccagtg gatctactga acaataactt gttatcaaac
      721 taagttgttt gatcaattta attgactcac cttaattcga tgcatatagt gtcatttttc
      781 taaaccaatg agttggccat gttagctgta ttccaacaat tgcaagattc cgcgcaactt
      841 ttaaagccct attttacgga ccataaaaat gcgtcaaaaa ataagtggct tttctttgga
      901 tttttggtag ggaatatact acaagctgtt gtcattgctg ttctttatac ctatatgtcg
      961 ctgtttatag gtatttttgc catgcccgtg ttaagttacg cccttttatt tagcactttg
     1021 gcagaatgct cttttgcttt acttgcttat accgcaacct cggtcgtcaa taattatctt
     1081 acgacttcat taaagtattc tctatcggaa agtcttaatg cagacttaag tgcgcaatgg
     1141 ctacgcaatc aaaattttta tggtataaaa caacctggaa ataacaccgt ttatcccgat
     1201 aaagtattat cggaagatag tataacgctc gttaagaaat cgatcgattt gttgcaatta
     1261 agttttaacg tcgcttttgg attcatcatt ggcgtttatc gtttatttgc tctttccgta
     1321 gtgacgacta ttaccgtttt caatcgtgaa attgcaatcc caggcttaat ggcaattggt
     1381 tctatagggt atgcacttgt atataattta atttcagcac gtgtcagtaa tagcttaacg
     1441 gcgcaagtga atgttgaaaa agaaaaaagt ggtcatatgc aaaaagtcat ggatcatatc
     1501 gatgcaaatc atggtgctct cgctatgcaa aatgctgccc atcgcgagca gggtttttac
     1561 aaccatgcct ttaatgattt aaaagacgca aaattaaaag tattaaacac aaccacaaaa
     1621 ctcgattttc tttctataat gaatgaatcc ctttcggtat tggttggctt aattctcgca
     1681 gcccctagta taatagccaa aaaaattgat gctacgtcca tgctagaaat aaaccattac
     1741 gttagtgaca ttattaaatt tttcaccgtt aagtacgata atattcatga cattacccaa
     1801 ctagaaactt ctgttcaacg tataaatgaa ttcaaaaagg cgatggcaac ttgggaaaaa
     1861 agttaccaaa agaatcagca ttttacatcc ccagaaagta gtttttccgt gtctaatctc
     1921 acgattaaaa atcctcgcaa tgaaacttta ttagataaag taaatgtcac ttttgaaaaa
     1981 ggaaagatta cactattaaa aggtgaatcc ggttgcggca aatccacgct gttaagaact
     2041 ctagctggac tttggccaaa agaaggcacc tattcgcttc cttttaagcg cgaagaaatt
     2101 gcaattatcc cgcaaacacc ctgctttcct tttagaagca agttaataga ggcgattgca
     2161 gaaaatccgg aatctattaa tcgtgatcaa ctaaaagcgt acatgaatga gcttgagctt
     2221 tcccatttaa ttccatacct tgacaaagaa ggttcatggt ctaccgtaag cgggggggaa
     2281 cgtcaacgtt tagcaatttt aggtatcttg actaaaactt cgcaacctaa attggttttc
     2341 ttggatgaat caactagtgc gttggatata aaattaaaag caaaaacgga agacttaatc
     2401 aaacgtgttt tgaaaaacac cactatttta gctgtcgatc ataatccgga gcacaccgca
     2461 aaatttcata aagcgccctt ttatgatacc acggtggaat tccgcaataa aaaactccat
     2521 acagcacacg cgccgagtaa cactccaaag cctgtaattt ttagcgaaaa acatccgaag
     2581 gcttctttgc caaaacagcc actgagcgcc aatttgcatc cttcctatca ttaaaaattc
     2641 ccccattagg gggattcttt taactacaat ttgatttttt agtgagataa atattttctg
     2701 cgtgtaactt ggtgataggt ttttgcaacg cttttgaaaa tttcagaaca gtgggtttat
     2761 gattgtagga tactggttca atactttgat tcctcaaatt ctccgatcta ttttgggtta
     2821 tattatattg atgcaaaatt tcttgttgct ttgcttgggt taagttcgga attacaagaa
     2881 tcgtaccatt ctcggttttt aacatgtgtg tgtccttgcc attagtttcg caagctttta
     2941 ttacgtcgtg tatattacga attttaatac cattcacttc tgcaattaat tctccttctg
     3001 ctttataacc aactgtgtag caagaggata aaattgttct gatagcgact aaatgatcct
     3061 gaggtttttc tttatcgcga tttgcaaaat agaggcgttt agcttgtatg aaattttgtg
     3121 aaacaggttg gacaaccagt tgagctgaaa taatataata cgtgggagga ttatcatgtt
     3181 ctgtaagaat actttcggtt gcccctatcg attgttctaa tttgacgtta agatctaaca
     3241 ttttaccttg tcgcaaaatg cgaaatcgta cggagtcacc gatagattta ttttgaatta
     3301 aataatgaaa gtccattttt ttcatagggt gcatacaaat cgagccatca aagtttaatg
     3361 gaacatcgtc gatggctaag ataatatcct ctttgcgtaa aattcctttg caactagata
     3421 aattggcaat agatttaatt aaaactcctg attgatgtga aagcaatttg aaatgttttc
     3481 tcagatagaa attttccaat cgttgtatgc taatccctag atccggaaat cctttttttt
     3541 gttgagaact ttgctttata aaatttgtca gaatattaac ggggatcata taacctaaat
     3601 tttgtgcccc tggatatcct tggtggacga cgcccacaac atcaccatta ctgttaagaa
     3661 caggacctcc actattaccg ggattaattg gcgcacttat ttgcgtagtt atgaattctc
     3721 tgccactgtg cgcataacga tcctgttcga tacgcgaaac tatacccttc gttatggaaa
     3781 ccgtctttcc acctagggga aatcctatca ctttcacttt ttcttcatgc ttcgggactg
     3841 atccgagctg taaagcttgg atcttacgcc aaaagtcttt attctcaact tccaaaatag
     3901 cgagatcaca ttcatgcgct atgtcttgta acgttgcgca atatttttta ctatcatgcg
     3961 ctaattgtat ctcgagatgg gtagcacctg ctgcaacatg cgcattcgta attaatttaa
     4021 ttccattatc actttgaact aaaaatccac tgcctgtcca acgcactatt tttggtgctc
     4081 gccatggaaa tttataatca tgttttaaag cggtagtaaa aatttttaca actgaagttt
     4141 ctgcttgcga cgcattggtt gtgggtggtt taacctgggc tcccttcatg ctctttatct
     4201 cctcctgcta cactatgaat cgtttcttag gggataattt cattgtcgcc aaaaaaaaca
     4261 tttttgtcta gcgctctaac taaaaattag aattgttgga ggatcgaagc aatttacaat
     4321 gtttaataac gattataaaa agaagcagct tgcctgcttc tttacatagt catagatttc
     4381 gcatcaagtt gaggcataat tttagccaaa ttgccggttg gagaatgcgt tttttcatca
     4441 aatattctcg tatccgcaaa aattatcaaa gattctttag cacgtgataa gacacggtaa
     4501 agcattgcat ccccacaatc taacaaaaag ggacgtggac tatagagcgt ataaactgga
     4561 gagaataaaa tagttttata tctatgctca ggacaatttt taagcaaata cacaggaacg
     4621 gaacaagcaa gcatgcggca ttgttcttct aggtagattt tttgtgcatt cgataacgta
     4681 attattgcca ggttgtctgc agaattttct ttaagccatg ttatcaaaca atcagcttct
     4741 tttgggttaa accaaccttc ttgatccaaa acttctccaa taatatggaa gaattttaat
     4801 ccagctccca aaagtggatg cacgatacat tttttatcga tataagtttt atagaaagcg
     4861 gccaaattag gttggcacca tatttcagaa aatgcgagag gtctgtcata atataatcct
     4921 gtaggatcat attgtttata ataactttgc tgctctgcca aataatacaa agaagtctgg
     4981 cttaatactt tgcctttata ctgtaaatct tctaatacct cttcagttgc aatacccatc
     5041 aatgttttta aagcgagaga atcgatatcg ctcaatacaa tggggtacga caaggttaat
     5101 tttggatgcc caaaaattaa cgctttttgc gcttgtcgca aaagtttaaa agacaaaggc
     5161 aacgagaaat catgcgcatg cgtcacgata agcaaatcaa attttttatc gatcgtatgt
     5221 gccattttta agtcggcagt attatagaat gttatatcga aatacgtttt tgaaccttgt
     5281 ctcgaactaa gcagctttac gccatgcaac aatttacttc caaaaatggt aaggagttga
     5341 ctccacgcat ctatccattg agtggtcgtt gataattgcg gatattgtcg acaaagacct
     5401 tgccaagtat tttgttgcga ttctaaatgc gcaatttcat ccgacaattc cgtcaacgtt
     5461 ttatggtttt ttgcacgttt cactttgagg gcgcgaatat tttcacttat attttcttcg
     5521 attgttaaat gttctttaaa agaaaaattt gaacaatatg ttttaagaaa tgtcgaaatt
     5581 cgctcttttt
//
LOCUS       DASTIB010000316         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_753982, whole genome shotgun sequence.
ACCESSION   DASTIB010000316 DASTIB010000000
VERSION     DASTIB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753982"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /gene="pheT"
                     /locus_tag="VFP93_05505"
     CDS             <1..1855
                     /gene="pheT"
                     /locus_tag="VFP93_05505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9844092.1"
                     /translation="ARELHALTKATLHPVRIESIAPHKDDTLTIHIEDEKACPRYCGR
                     IIKNIDCSKPSPDWLKEKLRRSGIRSINPIVDIMNYVMLELGQPMHAFDLDKIDHAIY
                     VRLSQEGEQLTLLDGKEVKIHPNTLTIADSNKPLALAGIMGGEESAVTDTTKNIFLES
                     AFFHPEAIVGKARSFGLHTDSSARFERGVDPNLQDSAIERATQWILDICGGEPCPITD
                     IKTQALQSHKSIKLAISKAKKLIGMDLEEKSIIQDLERLGCVVSNNSEDVWDVKIPSF
                     RFDLSIPEDLVEEIARCGGYDAITASLPKAQLKPNYEKKPFSEKRLKHALIDFGYHEA
                     ITYSFVDPKLQNLLFPEVEKIALSNPIASDLSQMRISLWPGLLKALQYNYHRQQNNIF
                     LFETGLVFQETAAGLVQEPKLGGVCLGSLLPEHWQEKPKAIDFYAIKGHLETLWGLNR
                     KDLAKLKWEATKHPALHPGQACHLVYKDKVVGILGALHPDIEKHLDIKAPIFVFELKL
                     KALLRSKSIRFKKPSVFPSIRRDLSMVIDANIPYEKLSNWIHGEAGNLLKKLVLFDIY
                     QGKNIKPGCKSVALGFIWQDPNRTLVDKEIDDIMQDLIQGLEQQFGAQLRE"
     gene            1857..>2119
                     /locus_tag="VFP93_05510"
     CDS             1857..>2119
                     /locus_tag="VFP93_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HET9844093.1"
                     /translation="MTLTKAELAEAIISKHNFNKKDAKDLVDLVFEELRLALERGDSV
                     KISGFGNFDLRDKNQRPGRNPKTGQDIPITARRVVTFKPGQKL"
BASE COUNT          683 a          367 c          454 g          615 t
ORIGIN      
        1 agcgcgcgag ttgcatgctt taacgaaagc tacgttacat cctgtgcgca ttgaaagcat
       61 agcaccccat aaagatgaca cattaactat tcatatcgaa gacgaaaagg cgtgcccacg
      121 ttattgtggc agaatcataa aaaacatcga ttgttctaag cctagtcctg attggttaaa
      181 agaaaaactg cgccgaagtg gtatacgatc cattaatccc attgtcgata ttatgaatta
      241 tgtcatgcta gagcttggtc agccgatgca tgcttttgat ttggataaaa tcgaccatgc
      301 aatttatgtg cgcctttcgc aagaagggga acagcttact ttacttgatg gtaaagaagt
      361 taaaatacac cctaacactt taacaatagc ggacagtaat aaacctttag cccttgcagg
      421 catcatgggt ggagaagaat ctgcagttac ggatactaca aaaaacattt tcttagaaag
      481 cgcttttttc catccggagg ctattgtcgg caaagcacgc agttttggct tgcataccga
      541 ttcttccgcg cgttttgaac gaggggttga tccgaattta caagatagtg cgatagagcg
      601 tgccacccaa tggattttag atatttgtgg gggcgagcct tgcccaatta ccgatataaa
      661 aacccaagcg ctacaatcac ataaatctat caaattagcg atatcaaaag ctaaaaaatt
      721 aattggcatg gatttagaag aaaaatccat cattcaggat ttagaacgat tggggtgtgt
      781 ggtttcgaat aatagtgaag acgtatggga tgtaaaaatt cctagttttc gttttgattt
      841 aagtattcca gaagatttgg tagaagaaat tgcacggtgt gggggttatg atgcaatcac
      901 agcttcttta cccaaagcac aacttaagcc taattacgag aaaaagcctt tttcagaaaa
      961 gcggcttaaa cacgcgctca ttgattttgg ttatcacgag gctatcacct atagctttgt
     1021 ggatcccaaa ttacagaatt tattatttcc tgaagttgag aaaatagcct taagcaatcc
     1081 aatcgctagt gatttatcgc aaatgcgtat tagtctttgg ccaggtttgt taaaagcttt
     1141 gcagtacaac taccatcgtc agcaaaataa tatttttctt tttgaaactg gtttagtgtt
     1201 tcaagaaact gcggcgggtt tggtacaaga gccaaagctg ggtggtgtat gcttaggtag
     1261 cttacttcct gagcattggc aagaaaaacc taaagctatc gatttttacg ctatcaaagg
     1321 gcaccttgag acattgtggg gtttaaatcg caaagattta gcgaaattaa agtgggaagc
     1381 taccaagcat ccagcgttac atccagggca agcgtgtcac ttggtttata aagataaggt
     1441 ggtagggata ttgggggcac tacatcctga tatagaaaaa catttagata taaaagcccc
     1501 tatttttgtc tttgaattaa aactaaaagc attgctacgt tccaaaagta tacggtttaa
     1561 aaaaccttcc gtgtttcctt cgattcgtcg cgatttgagc atggtgattg atgccaatat
     1621 tccttatgag aaattaagca attggattca tggtgaagca gggaatttat tgaaaaaatt
     1681 agttttattt gatatttatc aaggaaagaa tatcaaaccc ggttgcaaga gtgttgcttt
     1741 aggctttatt tggcaagatc caaatcgtac cttagtagat aaagaaatcg atgatattat
     1801 gcaagacctt attcaagggt tagagcaaca atttggggca caattaaggg aataacatga
     1861 cgttaacaaa ggcagagtta gctgaagcga taatctctaa gcataatttt aataaaaaag
     1921 atgcaaaaga tttagtggat ttggtatttg aagaattaag attggcgtta gagcgcggtg
     1981 attctgtcaa aatttcagga tttggtaatt tcgatttgcg agataaaaat caacgtcctg
     2041 gtcgcaaccc taaaacaggg caagatatcc ccattaccgc aagacgtgtt gttactttta
     2101 agcctggaca aaaattaaa
//
LOCUS       DASTIB010000317         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U822
            k141_1037312, whole genome shotgun sequence.
ACCESSION   DASTIB010000317 DASTIB010000000
VERSION     DASTIB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969945
            Sequence Read Archive: SRR25158428
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 20:30:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,106
            CDSs (total)                      :: 1,087
            Genes (coding)                    :: 1,078
            CDSs (with protein)               :: 1,078
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037312"
                     /isolate="SMAG_U822"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.31666667 N 114.9069444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFP93_05515"
     CDS             complement(<1..930)
                     /locus_tag="VFP93_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9844094.1"
                     /translation="MFDFKAQSINTLVKKLAKDPILLVDLASHTISQIKRYNPKFRPF
                     VFYDENTLNQLIPDLKKLKNPSDLFGIPFGVKDIFNTEDYPTQMGSDLWKDFTPGNDA
                     RIVHYLKQQGAIIAGKTETAEFAVHALGQTLNPHDVFRTPGTSSSGSAVAVALGMVPA
                     ALGSQTAGSIIRPASFCGVYGCKPSFGLMSRTGVLKTTDTLDQVGFFTYFAEDMKTIF
                     DAIRVKGKNYYYTHQHLNDSKRKQKKTNEPWRVAFVKTPTWPIAEDYARKNIANLLDN
                     MKSCTDFDVNEIELPNIIYDAYKHHEEIYHKSLA"
     gene            complement(945..2132)
                     /locus_tag="VFP93_05520"
     CDS             complement(945..2132)
                     /locus_tag="VFP93_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HET9844095.1"
                     /translation="MTYSTQQPFSELQYCIRCCMPETNEGIEFDELGYCTACQASEQK
                     MHINWAERRRELEKILENAKQKAGDNYDCIIPISGGKDSTFQLHLITKVFNMKPLAVT
                     FSHNWFSKIGFYNLMNALEKFNVDHIMYTPNRALVNKLAKHSLYGVGDSCWHCHSGVG
                     SFPLHIATKFKIPLLIWGESLAEMSGRGTHFNPIRKFDREYFTKVSAKLTPTQMKNDE
                     LSSKDLFMFNLPTAKECEDVALFGIHLGDYVFWDDERQVEFLIKEYDWKETEIEGAYK
                     RYKSAECIMPGVHDATCYLKRGFGRATWQASVDVRNGIMTREEGFKLINEVDPVRPDA
                     LDYYLDITGLKEQEYLNIMEKHRVEQLKGKSIPIKLKEPNQRKQIKPFIQSFIEEMQL
                     ETD"
     gene            complement(2148..2738)
                     /locus_tag="VFP93_05525"
     CDS             complement(2148..2738)
                     /locus_tag="VFP93_05525"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9844096.1"
                     /translation="MNERLAKRPNREIPLSSKKYIYQSATIGVRPLDYYDLQGGYCYW
                     FNDMATCRYNSHGNYPMHIATLQRYLDNLATDQSKCVWAIDDIHLSKHIGNISLQSIH
                     SIYRNAEFAIIIGESDYRGKQIGLMATRWLFKHGFERLNLHRIYCGTASTNQGMIKLA
                     EKLGMRNEGCLRDAAFLDGYYVDLIQFGILKKEFTV"
     gene            complement(2744..>3181)
                     /locus_tag="VFP93_05530"
     CDS             complement(2744..>3181)
                     /locus_tag="VFP93_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9844097.1"
                     /translation="ILIQIEKFKHAYGQHRIHILYVCEPIGANRSKYGYDEFEALHGY
                     LNYLCQHNERNKFSIKIRPHPREAADKYQSCIDLYRAKLDIILNAHESLAEAIAWSDT
                     VVGCETMAMVVALVADKKVYSAIPAHGRTCSLPHKNIIKLFKA"
BASE COUNT          937 a          638 c          533 g         1073 t
ORIGIN      
        1 agctagcgat ttatggtaaa tttcttcatg gtgcttatac gcatcataaa taatattagg
       61 caactcaatt tcattgacat cgaaatcggt gcatgatttc atattatcca aaagatttgc
      121 gatatttttc cttgcataat cttctgcaat aggccaggtt ggtgttttaa caaatgcaac
      181 acgccaaggc tcgttagttt ttttttgttt gcgtttacta tcgtttaaat gttgatgcgt
      241 ataataataa ttttttcctt tcacgcgtat tgcatcaaat atagttttca tgtcttcagc
      301 aaaataggta aagaaaccga cttgatcgag tgtatccgtt gtttttaaaa ctcccgttct
      361 agacattaaa ccaaaagaag gtttacatcc gtaaactcca cagaaacttg caggacgtat
      421 aatggaaccg gctgtttgag aacctaaagc cgcaggaacc ataccaagag cgacggcaac
      481 tgctgaacca cttgatgatg tcccaggcgt tcgaaaaaca tcatggggat tgagcgtttg
      541 acccagtgca tgcaccgcaa attcggcagt ttccgttttt ccagcaataa tcgcgccttg
      601 ttgcttaaga taatgaacaa ttctcgcatc attgcctggg gtaaaatctt tccataaatc
      661 gcttcccatt tgtgtaggat aatcttcagt attaaagata tctttgacac caaatggtat
      721 tccaaataaa tcactaggat tttttaactt tttaagatct ggaatcaatt ggttcaaagt
      781 gttttcatca taaaatacaa acggtcgaaa cttaggatta taccgtttaa tttgtgaaat
      841 ggtgtgcgag gctagatcaa ctaataaaat ggggtccttc gcaagtttct taaccaatgt
      901 attgatcgac tgcgctttaa aatcaaacat acctacctct tcaactaatc agtttcaagt
      961 tgcatttctt ctataaagct ttgaataaat ggtttgatct gctttcgttg atttggttct
     1021 ttcaacttaa tagggattga tttaccttta agctgttcaa ctctatgttt ttccatgatg
     1081 tttaaatatt cttgttcttt taaccctgtg atgtccaaat aatagtcaag cgcatctgga
     1141 cgaactggat ccacttcatt aattaattta aaaccttctt cgcgggtcat gattccattt
     1201 cttacatcca ctgaagcttg ccatgttgca cgtccgaaac ctcgttttaa atagcacgtt
     1261 gcgtcatgaa cacctggcat tatacattca gcacttttat atctcttata agcaccttca
     1321 atttctgttt ctttccaatc atactccttt attaaaaatt caacttggcg ttcatcatcc
     1381 caaaatacat aatcacccaa atgtatgcca aacaaagcaa catcttcaca ttcttttgcc
     1441 gttgggagat taaacataaa caaatctttt gaacttaatt catcattttt catctgggta
     1501 ggggttaatt tcgcggacac ctttgtaaag tattcgcgat caaattttcg aattggatta
     1561 aaatgagtgc ccctaccaga catttcggcg agtgattctc cccatattaa tagcggtatt
     1621 ttaaattttg ttgctatatg caaaggaaat gaaccaacgc cgctatgaca atgccaacag
     1681 gaatcgccaa caccatataa tgaatgttta gctaatttat ttactaacgc tctatttggg
     1741 gtgtacataa tatgatcaac gttaaatttt tcaagagcat tcatcaaatt gtaaaaacca
     1801 attttactaa accaattgtg gctaaaggtc actgccaatg gtttcatatt gaacactttc
     1861 gttattaggt gtagttggaa agtactatct ttacctccac taataggaat gatgcaatcg
     1921 tagttatctc cggctttctg ttttgcattt tcaagaatct tctctaactc tctcctgcgt
     1981 tcggcccaat taatatgcat tttttgttca gaagcttggc atgcagtaca atatcccaat
     2041 tcatcaaact caataccctc atttgtttcc ggcatacagc atcttatgca atactgaagc
     2101 tctgagaatg gttgttgtgt tgaatacgtc atagtgatgc tcttttttta cacagtaaat
     2161 tcttttttta atatgccaaa ttgtattaaa tcaacataat atccatcgag aaatgcggcg
     2221 tctcgcaaac agccttcatt acgcatcccg agcttttctg ccaattttat catgccctga
     2281 tttgttgaag cagtaccgca ataaatgcga tgcaaattca aacgttcaaa accatgttta
     2341 aataaccatc gtgttgccat taatccaatc tgctttccgc gataatcgga ctcgccgata
     2401 attatcgcaa attctgcatt acgatatatt gaatgtattg attgtagaga aatattacca
     2461 atgtgctttg ataaatgaat atcatcgatt gcccaaacac acttactttg atctgtcgca
     2521 agattgtcaa gatatctttg aagggtcgca atgtgcattg gataatttcc atgcgaatta
     2581 taacgacatg tagccatatc attgaaccaa taacaatatc caccctgaag atcataataa
     2641 tctaatggcc gcactccaat cgtagcagat tggtatatat acttttttga ggataatggg
     2701 atctctctgt taggtcgctt ggcaagcctt tcattcatat actctatgcc ttaaaaagtt
     2761 ttatgatgtt tttgtgtggt aaagagcaag ttcgaccatg agcaggaatt gctgagtata
     2821 cttttttatc cgctacaagc gcaactacca tcgccatagt ttcgcaaccg actacggtgt
     2881 ccgaccaagc tatagcttcg gctaaactct catgagcatt caatattata tctaactttg
     2941 ctctataaag gtctatgcag gattgatatt tatcagccgc ttcacgtgga tgtggtcgga
     3001 ttttaataga gaacttattt ctctcgttgt gttgacataa atagtttaaa tatccatgta
     3061 gtgcttcaaa ctcgtcatac ccatattttg atcgattcgc acctatcggt tcacaaacgt
     3121 atagaatatg tatacgatgt tgcccatagg cgtgcttgaa tttttcaatt tgaattagta
     3181 t
//
